1. Electron-microscopic observations of the Coccidioides immitis parasitic cycle in vivo.
- Author
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Sun SH, Cole GT, Drutz DJ, and Harrison JL
- Subjects
- Animals, Coccidioides physiology, Coccidioides ultrastructure, Coccidioidomycosis pathology, Freeze Fracturing, Lung ultrastructure, Lung Diseases, Fungal pathology, Mice, Mice, Inbred BALB C, Microscopy, Electron, Microscopy, Electron, Scanning, Morphogenesis, Spores, Fungal, Coccidioides growth & development, Coccidioidomycosis microbiology, Lung microbiology, Lung Diseases, Fungal microbiology
- Abstract
BALB/c mice were inoculated by intranasal challenge with viable arthroconidia of C. immitis and in-vivo morphogenesis of the fungal pathogen was investigated by electron-microscopic examination of pulmonary lavage and cryofractured lung specimens. Samples were prepared at intervals over an 11-day period. Stages of spherule and endospore development were easily identified by scanning and thin-section electron microscopy. Details of morphogenesis of the pathogen in vivo closely resemble developmental aspects reported from in-vitro studies.
- Published
- 1986