1. A spectrophotometric assay for measuring the uptake of actinobolin by components of human enamel.
- Author
-
Hunt DE, Kelling AL, and Hunt JK
- Subjects
- Antibiotics, Antineoplastic analysis, Binding Sites, Humans, In Vitro Techniques, Spectrophotometry, Anti-Bacterial Agents metabolism, Antibiotics, Antineoplastic metabolism, Dental Enamel metabolism, Hydroxyapatites metabolism
- Abstract
A spectrophotometric assay was developed for measuring the uptake of the antibiotic actinobolin by hydroxylapatite (HAP) or powdered human enamel. The assay is sufficiently sensitive to detect less than 2.0 mug actinobolin/ml of: 0.01 M sodium phosphate buffer at pH 5.5, 7.0, or 8.0; deionized water; deionized water containing 1% salivary supernatant; or each of the above indicated solvent systems containing 1-5 parts per million sodium fluoride. The utility of the assay system has been demonstrated by date which show that approximately 5-7 mug of actinobolin are bound per 10 mg of HAP or powdered enamel.
- Published
- 1976
- Full Text
- View/download PDF