1. Expansion of Lung Vα14 NKT Cells by Administration of α‐Galactosylceramide‐pulsed Dendritic Cells
- Author
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Toshinori Nakayama, Shinichiro Motohashi, Toshihiro Ito, Yukimasa Miyazawa, Noriaki Kamada, Masaru Taniguchi, Eiko Shimizu, Yasunori Akutsu, Takenori Ochiai, Takeshi Saito, Hisahiro Matsubara, Tetsu Kawano, and Michishige Harada
- Subjects
Cancer Research ,CD1 ,Cell Culture Techniques ,chemical and pharmacologic phenomena ,Antineoplastic Agents ,Galactosylceramides ,Mice, Transgenic ,Biology ,Ligands ,Article ,Natural killer cell ,Antigens, CD1 ,Mice ,Cell Line, Tumor ,medicine ,α‐Galactosylceramide ,Animals ,Cell Lineage ,Neoplasm Metastasis ,Antigen-presenting cell ,Lung ,Cells, Cultured ,Follicular dendritic cells ,Reverse Transcriptase Polymerase Chain Reaction ,Dendritic cell ,Dendritic Cells ,Natural killer T cell ,Chromium Radioisotopes ,Cell biology ,carbohydrates (lipids) ,Mice, Inbred C57BL ,medicine.anatomical_structure ,Oncology ,CD1D ,Immunology ,Interleukin 12 ,biology.protein ,Leukocytes, Mononuclear ,RNA ,lipids (amino acids, peptides, and proteins) ,Immunotherapy ,Antigens, CD1d ,NKT cell - Abstract
NKT cells, a novel murine lymphoid lineage bearing an invariant T cell receptor encoded by V alpha 14 and J alpha 281 gene segments, recognize a specific ligand glycolipid, alpha-galactosylceramide (alpha-GalCer) in a CD1d-dependent manner. Recent research has revealed that activated V alpha 14 NKT cells have dramatic antitumor effects against a wide variety of tumor cell lines in vivo and in vitro. Here, we demonstrate strong in vivo antitumor effects brought about by treatment with alpha-GalCer-pulsed dendritic cells in comparison with in vitro-activated V alpha 14 NKT cells. Furthermore, we show a significant expansion of endogenous V alpha 14 NKT cells in the lung following the administration of alpha-GalCer-pulsed dendritic cells. The feasibility of immunotherapy with alpha-GalCer-pulsed dendritic cells is discussed.
- Published
- 2002