1. CK-1, a putative chemokine of rainbow trout (Oncorhynchus mykiss).
- Author
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Dixon B, Shum B, Adams EJ, Magor KE, Hedrick RP, Muir DG, and Parham P
- Subjects
- Animals, Binding Sites, Chemokines classification, Chemokines, CC chemistry, Chemokines, CXC genetics, DNA, Complementary, Genetic Variation, Humans, Oncorhynchus mykiss immunology, Chemokines genetics, Fish Proteins, Oncorhynchus mykiss genetics
- Abstract
Chemokines are small inducible proteins that direct the migration of leukocytes. While chemokines are well characterised in mammals, they have yet to be identified in fish. We have isolated a cDNA clone from rainbow trout (Oncorhynchus mykiss) which encodes a protein (CK-1) having structural features typical of chemokines. Amino-acid residues that define the beta-chemokines of mammals are conserved in CK-1, including the paired cysteine motif, CC. Further similarities are shared with the C6 subfamily of beta-chemokines. In contrast, the organisation of the CK-1 gene is closer to that of mammalian alpha-chemokine genes than beta-chemokine genes. The CK-1 gene is present in all four salmonid species examined and the nucleotide sequences of the exons are highly conserved. CK-1 has characteristics in common with mammalian alpha and beta-chemokine genes, suggesting that this salmonid chemokine gene preserves traits once present in the ancestral chemokine gene from which modern mammalian chemokine genes evolved.
- Published
- 1998
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