1. Curcumin exerts a protective effect against premature ovarian failure in mice
- Author
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Qingting Hao, Heling Fu, Daorong Hou, Youjin Dai, Dan Bao, Qin Chen, Yuan Zheng, Yuan Yin, Xiaowei Nie, Yugui Cui, and Zhengjie Yan
- Subjects
Anti-Mullerian Hormone ,0301 basic medicine ,Hypothalamo-Hypophyseal System ,medicine.medical_specialty ,Curcumin ,medicine.medical_treatment ,Intraperitoneal injection ,SOD2 ,Apoptosis ,premature ovarian failure ,Primary Ovarian Insufficiency ,Protective Agents ,medicine.disease_cause ,d-galactose ,03 medical and health sciences ,Subcutaneous injection ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Internal medicine ,medicine ,oxidative stress ,Animals ,RNA, Messenger ,Gonads ,Molecular Biology ,Protein kinase B ,Cyclin-Dependent Kinase Inhibitor p16 ,Aldehydes ,Chemistry ,Research ,Ovary ,Deoxyguanosine ,Galactose ,medicine.disease ,Premature ovarian failure ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,8-Hydroxy-2'-Deoxyguanosine ,030220 oncology & carcinogenesis ,ovary aging ,Tyrosine ,Female ,Oxidative stress - Abstract
This study was designed to investigate the protective effect of curcumin againstd-galactose (d-gal)-induced premature ovarian failure (POF) in mice. A mouse POF model was induced by subcutaneous injection ofd-gal (200 mg/kg/day) daily for 42 days. Mice in the curcumin group received bothd-gal treatment and intraperitoneal injection of curcumin (100 mg/kg/day) for 42 days. Ovarian function, oxidative stress and apoptosis were evaluated. The P, E2 and SOD levels were higher, and the FSH, LH and MDA levels were significantly lower in the curcumin group than those in thed-gal group. The proportion of primordial follicles was also significantly higher in the curcumin group than that in thed-gal group. In addition, curcumin treatment afterd-gal administration resulted in significantly lowerSod2,Cat, 8-OhdG, 4-HNE, NTY and senescence-associated protein P16 expression levels, higherAmhexpression levels and less apoptosis in granulosa cells than was observed in thed-gal group. Moreover, the p-Akt, Nrf2 and HO-1 protein expression levels were significantly higher and the apoptosis-related cleaved caspase-3 and -9 protein expression levels were markedly lower in the curcumin group than in thed-gal group. In conclusion, curcumin effectively inhibitedd-gal-induced oxidative stress, apoptosis and ovarian injury via a mechanism involving the Nrf2/HO-1 and PI3K/Akt signaling pathways, suggesting that curcumin is a potential protective agent against POF.
- Published
- 2018
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