1. Water volume and osmotic behaviour of mouse spermatozoa determined by electron paramagnetic resonance
- Author
-
Jun Tao, Junying Du, Frederick W. Kleinhans, Peter Mazur, and John K. Critser
- Subjects
Male ,Embryology ,Sodium ,Analytical chemistry ,Mineralogy ,chemistry.chemical_element ,Osmolar Concentration ,law.invention ,Mice ,Endocrinology ,law ,Animals ,Spin label ,Electron paramagnetic resonance ,Water content ,Cryopreservation ,Mice, Inbred ICR ,Osmotic concentration ,Chemistry ,Cell Membrane ,Electron Spin Resonance Spectroscopy ,Water ,Obstetrics and Gynecology ,Cell Biology ,Spermatozoa ,Reproductive Medicine ,Volume (thermodynamics) ,Water volume ,Semen Preservation - Abstract
Experiments were conducted to determine the water volume and osmotic behaviour of mouse spermatozoa using an electron paramagnetic resonance technique using the spin label tempone, and the broadening agent potassium chromium oxalate. After a swim-up procedure, an average water volume of 43.3 micron3 of individual spermatozoa was obtained at 290 mosmol. If a water compartment of 59% is assumed, the total volume of mouse spermatozoa is 73.4 micron3. A plot of the relative water volume of mouse spermatozoa versus the reciprocal of buffer osmolality (Boyle van't Hoff plot) is linear in the range 250-900 mosmol of sodium chloride solutions (r2 = -.96). The Boyle van't Hoff plot intercept indicates that 13% of the spin-label accessible isotonic water is osmotically inactive.
- Published
- 1994
- Full Text
- View/download PDF