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Your search keyword '"Gilbert Isabelle"' showing total 9 results
Start Over Author "Gilbert Isabelle" Publisher bioscientifica
9 results on '"Gilbert Isabelle"'

1. Roles of the cumulus–oocyte transzonal network and the Fragile X protein family in oocyte competence

Author

Karen Nenonene, Elolo, primary, Trottier-Lavoie, Mallorie, additional, Marchais, Mathilde, additional, Bastien, Alexandre, additional, Gilbert, Isabelle, additional, Macaulay, Angus D, additional, Khandjian, Edouard W, additional, Maria Luciano, Alberto, additional, Lodde, Valentina, additional, Viger, Robert S, additional, and Robert, Claude, additional

Published
2023
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2. Roles of the cumulus-oocyte transzonal network and the Fragile X protein family in oocyte competence.

Author

Nenonene, Elolo Karen, Trottier-Lavoie, Mallorie, Marchais, Mathilde, Bastien, Alexandre, Gilbert, Isabelle, Macaulay, Angus D., Khandjian, Edouard W., Luciano, Alberto Maria, Lodde, Valentina, Viger, Robert S., and Robert, Claude

Subjects
GREEN fluorescent protein, OVUM, ZONA pellucida, RNA-binding proteins, NUCLEAR proteins, CYTOSKELETAL proteins
Abstract

The determinants of oocyte developmental competence have puzzled scientists for decades. It is known that follicular conditions can nurture the production of a high-quality oocyte, but the underlying mechanisms remain unknown. Somatic cumulus cells most proximal to the oocyte are known to have cellular extensions that reach across the zona pellucida and contact with the oocyte plasma membrane. Herein, it was found that transzonal projections (TZPs) network quality is associated with developmental competence. Knowing that ribonucleoparticles are abundant within TZPs, the distribution of RNA-binding proteins was studied. The Fragile X-related proteins (FXR1P and FXR2P) and two partnering protein families, namely cytoplasmic FMRP-interacting protein and nuclear FMRP-interacting protein, exhibited distinctive patterns consistent with roles in regulating mRNA packaging, transport, and translation. The expression of green fluorescent protein (GFP)-FMRP fusion protein in cumulus cells showed active granule formation and their transport and transfer through filipodia connecting with neighboring cells. Near the projections' ends was found the cytoskeletal anchoring protein Filamin A and active protein synthesis sites. This study highlights key proteins involved in delivering mRNA to the oocyte. Thus, cumulus cells appear to indeed support the development of high-quality oocytes via the transzonal network. [ABSTRACT FROM AUTHOR]

Published
2023
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4. The gametic synapse; transferring RNA to the oocyte

Author

Macaulay, Angus D, primary, Gilbert, Isabelle, additional, Caballero, Julieta, additional, Scantland, Sara, additional, Fournier, Eric, additional, Tossou, Prudencio, additional, Sirard, Marc-Andre, additional, Clarke, Hugh, additional, Khandjian, Edward, additional, Richard, Francois, additional, Hyttel, Poul, additional, and Robert, Claude, additional

Published
2014
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6. Comprehensive cross production system assessment of the impact of in vitro microenvironment on the expression of messengers and long non-coding RNAs in the bovine blastocyst

Author

Côté, Isabelle, primary, Vigneault, Christian, additional, Laflamme, Isabelle, additional, Laquerre, Joanie, additional, Fournier, Éric, additional, Gilbert, Isabelle, additional, Scantland, Sara, additional, Gagné, Dominic, additional, Blondin, Patrick, additional, and Robert, Claude, additional

Published
2011
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