20 results on '"da Cunha Maria"'
Search Results
2. Brazilian private health system: history, scenarios, and trends
- Author
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Cruz, June Alisson Westarb, da Cunha, Maria Alexandra Viegas Cortez, de Moraes, Thyago Proença, Marques, Sandro, Tuon, Felipe Francisco, Gomide, Arivelton Loeschke, and de Paula Linhares, Gisele
- Published
- 2022
- Full Text
- View/download PDF
3. Molecular characterization of methicillin-resistant Staphylococcus aureus among insulin-dependent diabetic individuals in Brazil
- Author
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Teixeira, Nathalia Bibiana, Castelo Branco Fortaleza, Carlos Magno, de Souza, Matheus Cristovam, Monteiro Pereira, Thais Aline, de Camargo Colenci, Bibiana Prada, and Ribeiro de Souza da Cunha, Maria de Lourdes
- Published
- 2021
- Full Text
- View/download PDF
4. Nasal colonization with methicillin-resistant Staphylococcus aureus among elderly living in nursing homes in Brazil: risk factors and molecular epidemiology
- Author
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da Silveira, Monica, da Cunha, Maria de Lourdes Ribeiro de Souza, de Souza, Camila Sena Martins, Correa, Adriana Aparecida Feltrin, and Fortaleza, Carlos Magno Castelo Branco
- Published
- 2018
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- View/download PDF
5. Crohn disease-like enterocolitis remission after empagliflozin treatment in a child with glycogen storage disease type Ib: a case report.
- Author
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Rossi, Alessandro, Miele, Erasmo, Fecarotta, Simona, Veiga-da-Cunha, Maria, Martinelli, Massimo, Mollica, Carmine, D'Armiento, Maria, Mozzillo, Enza, Strisciuglio, Pietro, Derks, Terry G. J., Staiano, Annamaria, and Parenti, Giancarlo
- Subjects
FECAL analysis ,CROHN'S disease ,ENTEROCOLITIS ,HEMOGLOBINS ,INFLAMMATORY bowel diseases ,EMPAGLIFLOZIN ,MAGNETIC resonance imaging ,NEUTROPHILS ,TREATMENT effectiveness ,GLYCOGEN storage disease ,QUALITY of life ,ABDOMEN ,DISEASE remission ,ANTIGENS ,RARE diseases ,DISEASE complications ,ADOLESCENCE - Abstract
Background: Besides major clinical/biochemical features, neutropenia and inflammatory bowel disease (IBD) constitute common complications of Glycogen storage disease type Ib (GSD Ib). However, their management is still challenging. Although previous reports have shown benefit of empagliflozin administration on neutropenia, no follow-up data on bowel (macro/microscopic) morphology are available. We herein present for the first time longitudinal assessment of bowel morphology in a GSD Ib child suffering from Crohn disease-like enterocolitis treated with empagliflozin. Case presentation: A 14-year-old boy with GSD Ib and severe IBD was (off-label) treated with empagliflozin (20 mg/day) after informed oral and written consent was obtained from the patient's parents. No adverse events were noted. Clinical symptoms and stool frequency improved within the first week of treatment. Pediatric Crohn disease activity index (PCDAI) normalised within the first month of treatment. Abdomen magnetic resonance imaging (MRI) performed 3 months after treatment initiation showed dramatic decrease in disease activity and length. Similar findings were reported on histology at 5.5 months. At 7.5 months hemoglobin levels normalised and fecal calprotectin almost normalised. Improved neutrophil count, metabolic control and quality of life were also noted. G-CSF dose was decreased by 33% and the patient was partly weaned from tube feeding. Conclusions: This is the first report presenting extensive gastrointestinal morphology follow-up in a GSD Ib patient receiving empagliflozin. The present case suggests that empagliflozin can be safe and effective in inducing IBD remission in GSD Ib patients and can even postpone surgery. Future studies are required to confirm its effect over time and assess its benefit in various disease stages. The development of an international collaborating networks for systematic data collection is worthy. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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- View/download PDF
6. Comparison of methods for the identification of microorganisms isolated from blood cultures.
- Author
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Marinho Monteiro, Aydir Cecília, Branco Fortaleza, Carlos Magno Castelo, Ferreira, Adriano Martison, de Souza Cavalcante, Ricardo, Mondelli, Alessandro Lia, Bagagli, Eduardo, and de Souza da Cunha, Maria de Lourdes Ribeiro
- Subjects
MICROBIOLOGY ,MICROORGANISMS ,BLOOD diseases ,BODY fluids ,HUMAN body - Abstract
Background: Bloodstream infections are responsible for thousands of deaths each year. The rapid identification of the microorganisms causing these infections permits correct therapeutic management that will improve the prognosis of the patient. In an attempt to reduce the time spent on this step, microorganism identification devices have been developed, including the VITEK® 2 system, which is currently used in routine clinical microbiology laboratories. Methods: This study evaluated the accuracy of the VITEK® 2 system in the identification of 400 microorganisms isolated from blood cultures and compared the results to those obtained with conventional phenotypic and genotypic methods. In parallel to the phenotypic identification methods, the DNA of these microorganisms was extracted directly from the blood culture bottles for genotypic identification by the polymerase chain reaction (PCR) and DNA sequencing. Results: The automated VITEK® 2 system correctly identified 94.7 % (379/400) of the isolates. The YST and GN cards resulted in 100 % correct identifications of yeasts (15/15) and Gram-negative bacilli (165/165), respectively. The GP card correctly identified 92.6 % (199/215) of Gram-positive cocci, while the ANC card was unable to correctly identify any Gram-positive bacilli (0/5). Conclusions: The performance of the VITEK® 2 system was considered acceptable and statistical analysis showed that the system is a suitable option for routine clinical microbiology laboratories to identify different microorganisms. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
7. Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups.
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Martins de Souza, Camila Sena, Castelo Branco Fortaleza, Carlos Magno, Lima Witzel, Claudia, Silveira, Mônica, Fávero Bonesso, Mariana, Alencar Marques, Silvio, and Ribeiro de Souza da Cunha, Maria de Lourdes
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TOXIGENIC fungi ,STAPHYLOCOCCUS aureus ,METHICILLIN-resistant staphylococcus aureus ,POLYMERASE chain reaction ,TOXIC shock syndrome treatment ,IMMUNOCOMPROMISED patients - Abstract
Background: Staphylococcus aureus is characterized by its pathogenicity and high prevalence, causing disease in both healthy and immunocompromised individuals due to its easy dissemination. This fact is aggravated by the widespread dissemination of S. aureus carrying toxigenic genes. The objective of this study was to determine the toxigenic profile of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in patients with purulent skin and/or soft tissue infections seen at the Dermatology Department of the University Hospital of the Botucatu Medical School, asymptomatic adults older than 60 years living in nursing homes, and prison inmates of the Avaré Detention Center. Methods: PCR was used for the detection of the mecA gene, enterotoxin genes (sea, seb, and sec), exfoliative toxins A and B (eta and etb), toxic shock syndrome toxin 1 (tst), panton-valentine leukocidin (lukS-PV and lukF-PV), and alphaand delta-hemolysins or cytotoxins (hla and hld). Results: The results showed a significant prevalence of toxigenic genes among S. aureus isolates from asymptomatic individuals, with the observation of a higher prevalence of cytotoxin genes. However, the panton-valentine leukocidin gene was only detected in MSSA isolated from patients with skin infections and the tst gene was exclusively found in MSSA isolated from prison inmates. Conclusions: The present study demonstrated a significant prevalence of toxigenic genes in MSSA and MRSA strains isolated from asymptomatic S. aureus carriers. There was a higher prevalence of cytotoxin genes. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
8. Characterization of the clonal profile of MRSA isolated in neonatal and pediatric intensive care units of a University Hospital.
- Author
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Pereira, Valéria Cataneli, Moraes Riboli, Danilo Flávio, and de Souza da Cunha, Maria de Lourdes Ribeiro
- Subjects
METHICILLIN-resistant staphylococcus aureus ,PATHOGENIC microorganisms ,NEONATOLOGY ,CRITICAL care medicine ,CHILDREN'S health - Abstract
Background Methicillin-resistant Staphylococcus aureus (MRSA) are important pathogens in neonatal and pediatric intensive care units, which can cause severe infections in hospitalized children. Detection of the mecA gene and classification of the staphylococcal cassette chromosome mec (SCCmec) permit the characterization of MRSA strains isolated from infections caused by these microorganisms. In contrast, pulsed-field gel electrophoresis (PFGE) is used to type MRSA clones. This method is commonly used to analyze the epidemiology of bacteria causing nosocomial infections. The objective of this study was to detect and characterize MRSA isolated from clinical specimens of children hospitalized in the neonatal and pediatric intensive care units of the University Hospital of the Botucatu Medical School. Methods A total of 119 S. aureus strains were isolated from clinical specimens and the mecA gene was detected by PCR. SCCmec was detected by multiplex PCR and the clonal profile was analyzed by PFGE. Results The mecA gene was detected in 17.6% (21/119) of the isolates; 42.9% (9/21) of MRSA were characterized as SCCmec type III and 57.1% (12/21) as type IV. Analysis of the clonal profile of these strains revealed three distinct clones, with SCCmec type III being related to the Brazilian endemic clone and type IV to clones JCSC4469 and USA800. Conclusions Replacement of clonal groups occurred in the neonatal and pediatric units over the period studied, a fact highlighting the importance of improving hygiene practices and control measures of nosocomial infections in these units. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
9. Nasopharyngeal carriage of Staphylococcus aureus among imprisoned males from Brazil without exposure to healthcare: risk factors and molecular characterization.
- Author
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de Lima Witzel, Claudia, Castelo Branco Fortaleza, Carlos Magno, de Souza, Camila Sena Martins, Moraes Riboli, Danilo Flávio, and Ribeiro de Souza da Cunha, Maria de Lourdes
- Subjects
METHICILLIN-resistant staphylococcus aureus ,MEDICAL care ,MASCULINITY ,PRISONERS - Abstract
Background Previous studies report high prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) colonization among imprisoned populations. However, there are no data on that prevalence in Brazilian correctional institutions. Findings We tested 302 male prisoners for nasopharyngeal colonization with Staphylococcus aureus from February 2009 through April 2010. The overall isolation rate of S. aureus was 16.5% (50/302). Men who had sex with men, users of inhalatory drugs and those with previous lung or skin diseases were more likely to be colonized with S. aureus. MRSA was isolated from 0.7% of subjects (2/302). The two Community-associated (CA)-MRSA belonged to ST5 but were unrelated based on the PFGE results. Both harbored SCCmec IV, and did not possess the Panton-Valentine Leukocidin gene. Conclusion We found low prevalence of S. aureus and CA-MRSA among prisoners. MRSA isolates ST5 from two subjects harboured SCCmec IV and presented different PFGE patterns. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
10. Diagnostic accuracy of semi-quantitative and quantitative culture techniques for the diagnosis of catheter-related infections in newborns and molecular typing of isolated microorganisms.
- Author
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Moraes Riboli, Danilo Flávio, Lyra, João César, Pessoa Silva, Eliane, Valadão, Luisa Leite, Bentlin, Maria Regina, Corrente, José Eduardo, Suppo de Souza Rugolo, Ligia Maria, and Ribeiro de Souza da Cunha, Maria de Lourdes
- Subjects
CATHETER-related infections ,NEONATAL intensive care ,DIAGNOSIS of neonatal diseases ,STAPHYLOCOCCUS epidermidis ,STAPHYLOCOCCUS aureus ,ESCHERICHIA coli ,YEAST ,COAGULASE - Abstract
Background Catheter-related bloodstream infections (CR-BSIs) have become the most common cause of healthcare-associated bloodstream infections in neonatal intensive care units (ICUs). Microbiological evidence implicating catheters as the source of bloodstream infection is necessary to establish the diagnosis of CR-BSIs. Semi-quantitative culture is used to determine the presence of microorganisms on the external catheter surface, whereas quantitative culture also isolates microorganisms present inside the catheter. The main objective of this study was to determine the sensitivity and specificity of these two techniques for the diagnosis of CR-BSIs in newborns from a neonatal ICU. In addition, PFGE was used for similarity analysis of the microorganisms isolated from catheters and blood cultures. Methods Semi-quantitative and quantitative methods were used for the culture of catheter tips obtained from newborns. Strains isolated from catheter tips and blood cultures which exhibited the same antimicrobial susceptibility profile were included in the study as positive cases of CRBSI. PFGE of the microorganisms isolated from catheters and blood cultures was performed for similarity analysis and detection of clones in the ICU. Results A total of 584 catheter tips from 399 patients seen between November 2005 and June 2012 were analyzed. Twenty-nine cases of CR-BSI were confirmed. Coagulase-negative staphylococci (CoNS) were the most frequently isolated microorganisms, including S. epidermidis as the most prevalent species (65.5%), followed by S. haemolyticus (10.3%), yeasts (10.3%), K. pneumoniae (6.9%), S. aureus (3.4%), and E. coli (3.4%). The sensitivity of the semi-quantitative and quantitative techniques was 72.7% and 59.3%, respectively, and specificity was 95.7% and 94.4%. The diagnosis of CR-BSIs based on PFGE analysis of similarity between strains isolated from catheter tips and blood cultures showed 82.6% sensitivity and 100% specificity. Conclusion The semi-quantitative culture method showed higher sensitivity and specificity for the diagnosis of CR-BSIs in newborns when compared to the quantitative technique. In addition, this method is easier to perform and shows better agreement with the gold standard, and should therefore be recommended for routine clinical laboratory use. PFGE may contribute to the control of CR-BSIs by identifying clusters of microorganisms in neonatal ICUs, providing a means of determining potential cross-infection between patients. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
11. Identification of type III secretion substrates of Chlamydia trachomatis using Yersinia enterocolitica as a heterologous system.
- Author
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da Cunha, Maria, Milho, Catarina, Almeida, Filipe, Pais, Sara V., Borges, Vítor, Maurício, Rui, Borrego, Maria José, Gomes, João Paulo, and Mota, Luís Jaime
- Subjects
- *
CHLAMYDIA , *YERSINIA enterocolitica , *SECRETION , *GENITOURINARY diseases , *CYTOSOL , *PROTEINS - Abstract
Background Chlamydia trachomatis is an obligate intracellular human pathogen causing ocular and urogenital infections that are a significant clinical and public health concern. This bacterium uses a type III secretion (T3S) system to manipulate host cells, through the delivery of effector proteins into their cytosol, membranes, and nucleus. In this work, we aimed to find previously unidentified C. trachomatis T3S substrates. Results We first analyzed the genome of C. trachomatis L2/434 strain for genes encoding mostly uncharacterized proteins that did not appear to possess a signal of the general secretory pathway and which had not been previously experimentally shown to be T3S substrates. We selected several genes with these characteristics and analyzed T3S of the encoding proteins using Yersinia enterocolitica as a heterologous system. We identified 23C. trachomatis proteins whose first 20 amino acids were sufficient to drive T3S of the mature form of β- lactamase TEM-1 by Y. enterocolitica. We found that 10 of these 23 proteins were also type III secreted in their full-length versions by Y. enterocolitica, providing additional support that they are T3S substrates. Seven of these 10 likely T3S substrates of C. trachomatis were delivered by Y. enterocolitica into host cells, further suggesting that they could be effectors. Finally, real-time quantitative PCR analysis of expression of genes encoding the 10 likely T3S substrates of C. trachomatis showed that 9 of them were clearly expressed during infection of host cells. Conclusions Using Y. enterocolitica as a heterologous system, we identified 10 likely T3S substrates of C. trachomatis (CT053, CT105, CT142, CT143, CT144, CT161, CT338, CT429, CT656, and CT849) and could detect translocation into host cells of CT053, CT105, CT142, CT143, CT161, CT338, and CT429. Therefore, we revealed several C. trachomatis proteins that could be effectors subverting host cell processes. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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12. Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis.
- Author
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França, Thais Graziela Donegá, Chiuso-Minicucci, Fernanda, Zorzella-Pezavento, Sofia Fernanda Gonçalves, Ishikawa, Larissa Lumi Watanabe, da Rosa, Larissa Camargo, Colavite, Priscila Maria, Marques, Camila, Ikoma, Maura Rosane Valério, da Cunha, Maria de Lourdes Ribeiro de Souza, and Sartori, Alexandrina
- Subjects
SUPERANTIGENS ,STAPHYLOCOCCUS aureus infections ,ENCEPHALOMYELITIS ,MYELIN oligodendrocyte glycoprotein ,CENTRAL nervous system ,T cells ,CYTOKINES - Abstract
Background Bacterial superantigens are potent T cell activators that can activate T cells with specificity for antigens of the central nervous system (CNS). In this study, we compared the effect of two S. aureus strains on experimental autoimmune encephalomyelitis (EAE) development. C57BL/6 female mice were infected with S. aureus ATCC 51650, which produces toxic shock syndrome toxin 1 (TSST-1+) or S. aureus ATCC 43300, which does not produce toxins (TOX-). Three days later, the animals were subjected to EAE induction by immunization with myelin oligodendrocyte glycoprotein (MOG). The weight variation, disease incidence and clinical score were recorded daily. Cytokines and Foxp3+ regulatory T cells in the brain were evaluated during the acute disease phase. Cytokines and Foxp3+ regulatory T cells in the spleen and histopathological analysis of the CNS were assessed during the chronic stage. Results Previous infection with both strains similarly decreased the clinical score; however, only the TSST-1+ strain clearly diminished inflammation in the CNS. The infections also modulated cytokine production in the spleen and CNS. Reduced production of IL-5 and IL-10 was detected in MOG-stimulated spleen cultures in the TOX- and TSST-1+ infected groups, respectively. In S. aureus stimulated cultures, there was an increased production of IFN-γ and IL-10 in both infected groups and an increased level of IL-5 in the TSST-1+ group. CNS infiltrating cell cultures from previously infected mice produced less IL-17 in response to MOG and more IFN-γ in response to S. aureus stimulation. Conclusions These results indicated that both strains attenuated clinical EAE manifestations, but only TSST-1 clearly decreased CNS inflammation. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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- View/download PDF
13. Differential arthritogenicity of Staphylococcus aureus strains isolated from biological samples.
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Colavite-Machado, Priscila Maria, Watanabe Ishikawa, Larissa Lumi, Donegá França, Thaís Graziela, Gonçalves Zorzella-Pezavento, Sofia Fernanda, da Rosa, Larissa Camargo, Chiuso-Minicucci, Fernanda, de Souza da Cunha, Maria de Lourdes Ribeiro, Garlet, Gustavo Pompermaier, and Sartori, Alexandrina
- Subjects
STAPHYLOCOCCUS aureus ,PHYSIOLOGIC strain ,OSTEOARTHRITIS ,SUPERANTIGENS ,BODY weight - Abstract
Background: Staphylococcus aureus is the most common agent of septic arthritis that is a severe, rapidly progressive and destructive joint disease. Superantigens produced by S. aureus are considered the major arthritogenic factors. In this study, we compared the arthritogenic potential of five superantigen-producing staphylococcal strains. Methods: Male C57BL/6 mice were intravenously infected with ATCC 19095 SEC
+ , N315 ST5 TSST-1+ , S-70 TSST-1+ , ATCC 51650 TSST-1+ and ATCC 13565 SEA+ strains. Clinical parameters as body weight, arthritis incidence and clinical score were daily evaluated. Joint histopathological analysis and spleen cytokine production were evaluated at the 14th day after infection. Results: Weight loss was observed in all infected mice. ATCC 19095 SEC+ , N315 ST5 TSST-1+ and S-70 TSST-1+ were arthritogenic, being the highest scores observed in ATCC 19095 SEC+ infected mice. Intermediate and lower clinical scores were observed in N315 ST5 TSST-1+ and S-70 TSST-1+ infected mice, respectively. The ATCC 13565 SEA+ strain caused death of 85% of the animals after 48 h. Arthritis triggered by the ATCC 19095 SEC+ strain was characterized by accentuated synovial hyperplasia, inflammation, pannus formation, cartilage destruction and bone erosion. Similar joint alterations were found in N315 ST5 TSST-1+ infected mice, however they were strikingly more discrete. Only minor synovial proliferation and inflammation were triggered by the S-70 TSST-1+ strain. The lowest levels of TNF-α, IL-6 and IL-17 production in response to S. aureus stimulation were found in cultures from mice infected with the less arthritogenic strains (S-70 TSST-1+ and ATCC 51650 TSST-1+ ). The highest production of IL-17 was detected in mice infected with the most arthritogenic strains (ATCC 19095 SEC+ and N315 ST5 TSST-1+ ). Conclusions: Together these results demonstrated that S. aureus strains, isolated from biological samples, were able to induce a typical septic arthritis in mice. These results also suggest that the variable arthritogenicity of these strains was, at least in part, related to their differential ability to induce IL-17 production. [ABSTRACT FROM AUTHOR]- Published
- 2013
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14. Effect of the yellow passion fruit peel flour (Passiflora edulis f. flavicarpa deg.) in insulin sensitivity in type 2 diabetes mellitus patients.
- Author
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do Socoro Ramos de Queiroz, Maria, Idalino Janebro, Daniele, Auxiliadora Lins da Cunha, Maria, dos Santos Medeiros, Josimar, Sabaa-Srur, Armando U. O., de Fatima FM Diniz, Margareth, and dos Santos, Silvana Cristina
- Subjects
TYPE 2 diabetes ,BLOOD sugar ,INSULIN resistance ,GLYCOSYLATED hemoglobin ,PEOPLE with diabetes - Abstract
Background: A study with the yellow passion fruit peel flour showed positive action in blood glucose control as therapies' adjuvant in patients with type 2 diabetes mellitus. Therefore, we evaluated its effect on insulin sensitivity since there is a quest for studies that focus at better understanding of insulin resistance aspects in diabetic patients. Furthermore its relationship with chronic complications can also give good prospects for alternative treatments. Methods: A total of 43 type 2 diabetes volunteers (28 females and 15 males) ingested 30 g/day of the yellow passion fruit peel flour for two months. The levels of blood glucose and fasting insulin, HOMA index and glycated hemoglobin were measured for each patient before and after dietary supplementation. Results: There was a significant difference in the fasting blood glucose values (P = 0.000) and glycated hemoglobin (P = 0.032) after supplementation. It was also seen a reduction in HOMA IR (P = 0.005) in the supplemented group, however it was not observed changes in insulin values for females. HOMA beta (P = 0.000) showed significant increase in its values for the studied group. Conclusions: The supplementation used decreased insulin resistance in type 2 diabetic patients, suggesting a positive action in blood glucose control as adjuvant therapy in conventional treatments. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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15. Nasal colonization with methicillin-resistant <italic>Staphylococcus aureus</italic> among elderly living in nursing homes in Brazil: risk factors and molecular epidemiology.
- Author
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da Silveira, Monica, da Cunha, Maria de Lourdes Ribeiro de Souza, de Souza, Camila Sena Martins, Correa, Adriana Aparecida Feltrin, and Fortaleza, Carlos Magno Castelo Branco
- Subjects
METHICILLIN-resistant staphylococcus aureus ,DISEASES in older people ,INFECTION ,NURSING care facilities ,EPIDEMIOLOGY - Abstract
Background: Methicillin-resistant
Staphylococcus aureus poses a threat to elderly living in nursing homes. Studies focusing on the epidemiology of colonization may help in the design of infection control strategies. Objective: To identify factors associated with MRSA colonization and the dissemination of clones among nursing home residents. Methods: Nasal swabs were collected from 300 persons from nine nursing homes in the city of Bauru, Brazil. Resistance to methicillin was identified through amplification of themec A gene. Strain typing (Pulsed-Field Gel Electrophoresis) and characterization of theStaphylococcal Chromosome Cassette (SCC)mec was performed. Univariate and multivariable models were used to identify predictors of overallS. aureus and MRSA carriage. Results: Rates ofS. aureus and MRSA colonization were 17.7 and 3.7%, respectively. Age and recent admission to a hospital were independently associated with colonization withS. aureus . MRSA colonization was associated with living in small (< 15 residents) and medium-sized (15–49 residents) facilities, as well as with recent hospitalization. Most MRSA strains carried SCCmec types II or IV, and there was evidence of clonal spread within and among different facilities. Conclusions: MRSA may be introduced in nursing homes form hospitals or arise from the community setting. Screening for asymptomatic colonization may identify persons with greater risk for infection, and is advised for residents discharged from acute care hospitals. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
16. Diagnostic accuracy of semi-quantitative and quantitative culture techniques for the diagnosis of catheter-related infections in newborns and molecular typing of isolated microorganisms.
- Author
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Riboli, Danilo Flávio Moraes, Lyra, Joao César, Silva, Eliane Pessoa, Valadao, Luisa Leite, Bentlin, Maria Regina, Corrente, José Eduardo, Suppo de Souza Rugolo, Ligia Maria, Ribeiro de Souza da Cunha, Maria de Lourdes, Lyra, João César, Valadão, Luisa Leite, Rugolo, Ligia Maria Suppo de Souza, and da Cunha, Maria de Lourdes Ribeiro de Souza
- Abstract
Background: Catheter-related bloodstream infections (CR-BSIs) have become the most common cause of healthcare-associated bloodstream infections in neonatal intensive care units (ICUs). Microbiological evidence implicating catheters as the source of bloodstream infection is necessary to establish the diagnosis of CR-BSIs. Semi-quantitative culture is used to determine the presence of microorganisms on the external catheter surface, whereas quantitative culture also isolates microorganisms present inside the catheter. The main objective of this study was to determine the sensitivity and specificity of these two techniques for the diagnosis of CR-BSIs in newborns from a neonatal ICU. In addition, PFGE was used for similarity analysis of the microorganisms isolated from catheters and blood cultures.Methods: Semi-quantitative and quantitative methods were used for the culture of catheter tips obtained from newborns. Strains isolated from catheter tips and blood cultures which exhibited the same antimicrobial susceptibility profile were included in the study as positive cases of CR-BSI. PFGE of the microorganisms isolated from catheters and blood cultures was performed for similarity analysis and detection of clones in the ICU.Results: A total of 584 catheter tips from 399 patients seen between November 2005 and June 2012 were analyzed. Twenty-nine cases of CR-BSI were confirmed. Coagulase-negative staphylococci (CoNS) were the most frequently isolated microorganisms, including S. epidermidis as the most prevalent species (65.5%), followed by S. haemolyticus (10.3%), yeasts (10.3%), K. pneumoniae (6.9%), S. aureus (3.4%), and E. coli (3.4%). The sensitivity of the semi-quantitative and quantitative techniques was 72.7% and 59.3%, respectively, and specificity was 95.7% and 94.4%. The diagnosis of CR-BSIs based on PFGE analysis of similarity between strains isolated from catheter tips and blood cultures showed 82.6% sensitivity and 100% specificity.Conclusion: The semi-quantitative culture method showed higher sensitivity and specificity for the diagnosis of CR-BSIs in newborns when compared to the quantitative technique. In addition, this method is easier to perform and shows better agreement with the gold standard, and should therefore be recommended for routine clinical laboratory use. PFGE may contribute to the control of CR-BSIs by identifying clusters of microorganisms in neonatal ICUs, providing a means of determining potential cross-infection between patients. [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
17. Comparison of methods for the identification of microorganisms isolated from blood cultures.
- Author
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Monteiro AC, Fortaleza CM, Ferreira AM, Cavalcante Rde S, Mondelli AL, Bagagli E, and da Cunha Mde L
- Subjects
- Automation, Laboratory, Bacterial Typing Techniques instrumentation, Blood Culture methods, DNA Primers chemical synthesis, DNA, Bacterial isolation & purification, DNA, Fungal isolation & purification, Fungi isolation & purification, Gram-Negative Bacteria isolation & purification, Gram-Positive Bacteria isolation & purification, Humans, Polymerase Chain Reaction standards, Sensitivity and Specificity, Sequence Analysis, DNA, Bacterial Typing Techniques standards, Blood Culture instrumentation, Fungi classification, Gram-Negative Bacteria classification, Gram-Positive Bacteria classification
- Abstract
Background: Bloodstream infections are responsible for thousands of deaths each year. The rapid identification of the microorganisms causing these infections permits correct therapeutic management that will improve the prognosis of the patient. In an attempt to reduce the time spent on this step, microorganism identification devices have been developed, including the VITEK(®) 2 system, which is currently used in routine clinical microbiology laboratories., Methods: This study evaluated the accuracy of the VITEK(®) 2 system in the identification of 400 microorganisms isolated from blood cultures and compared the results to those obtained with conventional phenotypic and genotypic methods. In parallel to the phenotypic identification methods, the DNA of these microorganisms was extracted directly from the blood culture bottles for genotypic identification by the polymerase chain reaction (PCR) and DNA sequencing., Results: The automated VITEK(®) 2 system correctly identified 94.7 % (379/400) of the isolates. The YST and GN cards resulted in 100 % correct identifications of yeasts (15/15) and Gram-negative bacilli (165/165), respectively. The GP card correctly identified 92.6 % (199/215) of Gram-positive cocci, while the ANC card was unable to correctly identify any Gram-positive bacilli (0/5)., Conclusions: The performance of the VITEK(®) 2 system was considered acceptable and statistical analysis showed that the system is a suitable option for routine clinical microbiology laboratories to identify different microorganisms.
- Published
- 2016
- Full Text
- View/download PDF
18. Nasopharyngeal carriage of Staphylococcus aureus among imprisoned males from Brazil without exposure to healthcare: risk factors and molecular characterization.
- Author
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Witzel Cde L, Fortaleza CM, de Souza CS, Riboli DF, and da Cunha Mde L
- Subjects
- Brazil epidemiology, Carrier State microbiology, Cluster Analysis, Cross-Sectional Studies, Electrophoresis, Gel, Pulsed-Field, Humans, Male, Molecular Epidemiology, Prevalence, Risk Factors, Staphylococcal Infections microbiology, Staphylococcus aureus classification, Staphylococcus aureus genetics, Carrier State epidemiology, Molecular Typing, Nasopharynx microbiology, Prisoners, Staphylococcal Infections epidemiology, Staphylococcus aureus isolation & purification
- Abstract
Background: Previous studies report high prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) colonization among imprisoned populations. However, there are no data on that prevalence in Brazilian correctional institutions., Findings: We tested 302 male prisoners for nasopharyngeal colonization with Staphylococcus aureus from February 2009 through April 2010. The overall isolation rate of S. aureus was 16.5% (50/302). Men who had sex with men, users of inhalatory drugs and those with previous lung or skin diseases were more likely to be colonized with S. aureus. MRSA was isolated from 0.7% of subjects (2/302). The two Community-associated (CA)-MRSA belonged to ST5 but were unrelated based on the PFGE results. Both harbored SCCmec IV, and did not possess the Panton-Valentine Leukocidin gene., Conclusion: We found low prevalence of S. aureus and CA-MRSA among prisoners. MRSA isolates ST5 from two subjects harboured SCCmec IV and presented different PFGE patterns.
- Published
- 2014
- Full Text
- View/download PDF
19. Effect of the yellow passion fruit peel flour (Passiflora edulis f. flavicarpa deg.) in insulin sensitivity in type 2 diabetes mellitus patients.
- Author
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de Queiroz Mdo S, Janebro DI, da Cunha MA, Medeiros Jdos S, Sabaa-Srur AU, Diniz Mde F, and Dos Santos SC
- Subjects
- Aged, Body Mass Index, Brazil, Combined Modality Therapy, Diabetes Mellitus, Type 2 complications, Diabetes Mellitus, Type 2 drug therapy, Diabetes Mellitus, Type 2 metabolism, Dietary Fiber therapeutic use, Drug Therapy, Combination, Female, Glycated Hemoglobin analysis, Humans, Male, Middle Aged, Overweight complications, Sex Characteristics, Diabetes Mellitus, Type 2 diet therapy, Dietary Supplements, Fruit chemistry, Hyperglycemia prevention & control, Hypoglycemic Agents therapeutic use, Insulin Resistance, Passiflora chemistry
- Abstract
Background: A study with the yellow passion fruit peel flour showed positive action in blood glucose control as therapies' adjuvant in patients with type 2 diabetes mellitus. Therefore, we evaluated its effect on insulin sensitivity since there is a quest for studies that focus at better understanding of insulin resistance aspects in diabetic patients. Furthermore its relationship with chronic complications can also give good prospects for alternative treatments., Methods: A total of 43 type 2 diabetes volunteers (28 females and 15 males) ingested 30 g/day of the yellow passion fruit peel flour for two months. The levels of blood glucose and fasting insulin, HOMA index and glycated hemoglobin were measured for each patient before and after dietary supplementation., Results: There was a significant difference in the fasting blood glucose values (P = 0.000) and glycated hemoglobin (P = 0.032) after supplementation. It was also seen a reduction in HOMA IR (P = 0.005) in the supplemented group, however it was not observed changes in insulin values for females. HOMA beta (P = 0.000) showed significant increase in its values for the studied group., Conclusions: The supplementation used decreased insulin resistance in type 2 diabetic patients, suggesting a positive action in blood glucose control as adjuvant therapy in conventional treatments.
- Published
- 2012
- Full Text
- View/download PDF
20. Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection.
- Author
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França TG, Ishikawa LL, Zorzella-Pezavento SF, Chiuso-Minicucci F, Guerino CP, da Cunha Mde L, and Sartori A
- Subjects
- Animals, Colony Count, Microbial, Female, Lung microbiology, Mice, Mice, Inbred BALB C, Staphylococcal Infections microbiology, Staphylococcal Infections pathology, Bacterial Vaccines immunology, Lung pathology, Malnutrition, Methicillin-Resistant Staphylococcus aureus physiology, Staphylococcal Infections physiopathology, Staphylococcal Infections prevention & control
- Abstract
Background: Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with emphasis on the lung involvement., Results: BALB/c mice submitted to a 20% dietary restriction during 20 days presented a significant decrease in body weight, lymphocyte number and also atrophy in thymus and intestinal epithelium. Determination of bacterial load by the number of colony forming units (CFU) indicated a similar susceptibility whereas the findings of Gram stain clearly suggested a higher amount of bacteria in the lungs of normal mice than in the undernourished ones. Immunization reduced bacterial growth in the lungs of normal mice but not in the undernourished ones. Histopathological analysis showed that inflammation appeared in the lungs from normal mice only after infection and that immunization prevented this pulmonary inflammatory process. On the other hand, undernourished mice presented lung inflammation even before infection. In addition, the degree of this inflammatory process did not change with infection or previous immunization., Conclusion: Our results indicated that lung injury during MRSA infection is prevented by previous immunization in well nourished but not in undernourished mice.
- Published
- 2009
- Full Text
- View/download PDF
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