1. Production of human blood group B antigen epitope conjugated protein in Escherichia coli and utilization of the adsorption blood group B antibody.
- Author
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Wenjing Shang, Yafei Zhai, Zhongrui Ma, Gongjin Yang, Yan Ding, Donglei Han, Jiang Li, Houcheng Zhang, Jun Liu, Peng George Wang, Xian-wei Liu, and Min Chen
- Subjects
IMMUNOADSORPTION ,ADSORPTION (Chemistry) ,TRANSPLANTATION of organs, tissues, etc. ,BLOOD plasma ,IMMUNE response - Abstract
Background: In the process of ABO-incompatible (ABOi) organ transplantation, removal of anti-A and/or B antibodies from blood plasma is a promising method to overcome hyperacute rejection and allograft loss caused by the immune response between anti-A and/or B antibodies and the A and/or B antigens in the recipient. Although there are commercial columns to do this work, the application is still limited because of the high production cost. Results: In this study, the PglB glycosylation pathway from Campylobacter jejuni was exploited to produce glycoprotein conjugated with Escherichia coli O86:B7 O-antigen, which bears the blood group B antigen epitope to absorb blood group B antibody in blood. The titers of blood group B antibody were reduced to a safe level without changing the clotting function of plasma after glycoprotein absorption of B antibodies in the plasma. Conclusions: We developed a feasible strategy for the specific adsorption/removal of blood group antibodies. This method will be useful in ABOi organ transplantation and universal blood transfusion. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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