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Your search keyword '"YunLong Pan"' showing total 2 results
Start Over Author "YunLong Pan" Publisher biomed central
2 results on '"YunLong Pan"'

1. Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis

Author

Yunlong Pan, Li Qin, Yin Leng, Hui Ding, Zhixian Chen, and Xiaoxu Zhao

Subjects
MAPK/ERK pathway, Cancer Research, Angiogenesis, Vimentin, MicroRNA-29b, lcsh:RC254-282, 03 medical and health sciences, 0302 clinical medicine, Genetics, medicine, Gene silencing, Vasculogenic mimicry, MTT assay, Epithelial–mesenchymal transition, lcsh:QH573-671, 030304 developmental biology, 0303 health sciences, biology, Chemistry, lcsh:Cytology, ETV4, Cancer, medicine.disease, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Colorectal cancer, ERK signaling pathway, Oncology, 030220 oncology & carcinogenesis, Cancer research, biology.protein, Primary Research
Abstract

Background Recent studies have reported the involvement of microRNA-29 (miR-29) family members in human cancers through their ability to regulate cellular functions. The present study investigated biological function of miR-29b in colorectal cancer (CRC). Methods CRC tissues and adjacent normal tissues were collected and the expression of ETV4 and miR-29b in the tissues were identified. The relationship between ETV4 and miR-29b or ETV4 expression and the EGFR promoter was identified using dual-luciferase reporter gene and CHIP assays. The proliferation, invasion, migration, and apoptosis of CRC HCT116 cells were assayed using MTT assay, Scratch test, Transwell assay, and flow cytometry, respectively. Also, expression of epithelial-mesenchymal transition (EMT) markers, angiogenic factors, and vasculogenic mimicry formation were evaluated using RT-qPCR and Western blot. Results ETV4 was upregulated, while miR-29b expression was decreased in CRC tissues. ETV4 was identified as a target gene of miR-29b, which in turn inactivated the ERK signaling pathway by targeting ETV4 and inhibiting EGFR transcription. Transfection with miR-29b mimic, siRNA-ETV4, or ERK signaling pathway inhibitor U0126 increased expression of E-cadherin and TSP-1, and CRC cell apoptosis, yet reduced expression of ERK1/2, MMP-2, MMP-9, Vimentin, and VEGF, as well as inhibiting EMT, angiogenesis, and CRC cell migration and invasion. The EMT, angiogenesis and cancer progression induced by miR-29b inhibitor were reversed by siRNA-mediated ETV4 silencing. Conclusions miR-29b suppresses angiogenesis and EMT in CRC via the ETV4/ERK/EGFR axis.

Published
2021

2. Long-term effects of laparoscopic sleeve gastrectomy versus roux-en-Y gastric bypass for the treatment of Chinese type 2 diabetes mellitus patients with body mass index 28-35 kg/m².

Author

Jingge Yang, Cunchuan Wang, Guo Cao, Wah Yang, Shuqing Yu, Hening Zhai, and Yunlong Pan

Subjects
TYPE 2 diabetes treatment, BARIATRIC surgery, GASTRECTOMY, GASTRIC bypass, LAPAROSCOPIC surgery, BODY mass index, DYSLIPIDEMIA, HEALTH of Chinese people
Abstract

Background: To compare long term effects of two bariatric procedures for Chinese type 2 diabetes mellitus (T2DM) patients with a body mass index (BMI) of 28-35 kg/m². Methods: Sixty four T2DM patients with Glycated hemoglobin A1c (HbA1c) ≧ 7.0 % were randomly assigned to receive laparoscopic sleeve gastrectomy (SG) or Roux-en-Y gastric bypass (RYGB) procedure. Weight, percentage of excess weight loss (%EWL), BMI, waist circumference, HbA1c, fasting blood glucose (FBG), and C-peptide were measured. Serum lipid levels were also measured during three-year postoperative follow-up visits. Results: Fifty five patients completed the 36-month follow-up. Both groups had similar baseline anthropometric and biochemical measures. At the end point, 22 patients (78.6 %) in SG group and 23 patients (85.2 %) in RYGB group achieved complete remission of diabetes mellitus with HbA1c < 6.0 % (P = 0.525) and without taking diabetic medications, and 25 patients in each group (89.3 % vs. 92.6 %) gained successful treatment of diabetes with HbA1c 6.5 % (P = 0.100). Change in HbA1c, FBG and C peptide were comparable in the two groups. The RYGB group had significantly greater weight loss than the SG group [percentage of total weight loss (%TWL) of 31.0 % vs. 27.1 % (P = 0.049), %EWL of 92.3 % vs. 81.9 % (P = 0.003), and change in BMI of 11.0 vs. 9.1 kg/m² (P = 0.017), respectively]. Serum lipids in each group were also greatly improved. Conclusion: In this three-year study, SG had similar positive effects on diabetes and dyslipidemia compared to RYGB in Chinese T2DM patients with BMI of 28-35 kg/m². Longer term follow-ups and larger sample studies are needed to confirm these outcomes, however. [ABSTRACT FROM AUTHOR]

Published
2015
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