157 results on '"Xin Lin"'
Search Results
2. The role of miR1 and miR133a in new-onset atrial fibrillation after acute myocardial infarction
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Zeng, Qingyi, Li, Wei, Luo, Zhenghua, Zhou, Haiyan, Duan, Zhonggang, and Xiong, Xin Lin
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- 2023
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3. Anal fistulotomy with one-stage shaped skin grafting for intersphincter anal fistulas: study protocol on a multicentre randomised controlled trial
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Zhao, Yongchang, Xie, Wei, Wu, Xiaoshuo, Li, Xiaolan, Guo, Jinyan, Cao, Qiurui, Liang, Jiadi, Chen, Xin-lin, Zhao, Wentao, Sun, Feng, Li, Hongjie, Luo, Weimin, and Li, Yuying
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- 2023
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4. Correction to: Young Sca-1 + bone marrow stem cell-derived exosomes preserve visual function via the miR-150-5p/MEKK3/JNK/c-Jun pathway to reduce M1 microglial polarization
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Wang, Yuan, Qin, Wan-yun, Wang, Qi, Liu, Xin-na, Li, Xiang-hui, Ye, Xin-qi, Bai, Ying, Zhang, Yan, Liu, Pan, Wang, Xin-lin, Zhou, Yu-hang, Yuan, Hui-ping, and Shao, Zheng-bo
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- 2023
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5. Young Sca-1+ bone marrow stem cell-derived exosomes preserve visual function via the miR-150-5p/MEKK3/JNK/c-Jun pathway to reduce M1 microglial polarization
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Wang, Yuan, Qin, Wan-yun, Wang, Qi, Liu, Xin-na, Li, Xiang-hui, Ye, Xin-qi, Bai, Ying, Zhang, Yan, Liu, Pan, Wang, Xin-lin, Zhou, Yu-hang, Yuan, Hui-ping, and Shao, Zheng-bo
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- 2023
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6. Cancer functional states-based molecular subtypes of gastric cancer
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Zhou, Qi, Yuan, Yiwu, Lu, Hao, Li, Xueqin, Liu, Ziyang, Gan, Jinheng, Yue, Zhenqi, Wu, Jiping, Sheng, Jie, and Xin, Lin
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- 2023
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7. Mutagenesis of the odorant receptor co-receptor (Orco) reveals severe olfactory defects in the crop pest moth Helicoverpa armigera
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Fan, Xiao-Bin, Mo, Bao-Tong, Li, Guo-Cheng, Huang, Ling-Qiao, Guo, Hao, Gong, Xin-Lin, and Wang, Chen-Zhu
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- 2022
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8. Propionate and butyrate attenuate macrophage pyroptosis and osteoclastogenesis induced by CoCrMo alloy particles
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Wu, Yang-Lin, Zhang, Chen-Hui, Teng, Yun, Pan, Ying, Liu, Nai-Cheng, Liu, Pei-Xin, Zhu, Xu, Su, Xin-Lin, and Lin, Jun
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- 2022
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9. Translation and evaluation of the simplified Chinese version of the rating form of IBD patient concerns
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Luo, Jianfeng, Zhong, Jiamin, Li, Haiwen, Zhang, Shijing, Zhang, Liwan, Hou, Jiang-tao, Ke, Junyu, Li, Huibiao, Liu, Fengbin, and Chen, Xin-lin
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- 2022
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10. Prognostic signature composed of transcription factors accurately predicts the prognosis of gastric cancer patients
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Zhou, Liqiang, Chen, Zhiqing, Wu, You, Lu, Hao, and Xin, Lin
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- 2021
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11. Thymosin β4 reverses phenotypic polarization of glial cells and cognitive impairment via negative regulation of NF-κB signaling axis in APP/PS1 mice
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Wang, Meng, Feng, Li-Rong, Li, Zi-Long, Ma, Kai-Ge, Chang, Ke-Wei, Chen, Xin-Lin, Yang, Peng-Bo, Ji, Sheng-Feng, Ma, Yan-Bing, Han, Hua, Ruganzua, John Bosco, Yang, Wei-Na, and Qian, Yi-Hua
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- 2021
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12. Asymmetric cortical development and prognosis in fetuses with isolated mild fetal ventriculomegaly: an observational prospective study
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Zhu, Rong, Chen, Jun Ya, Hou, Xin Lin, Liu, Li Li, and Sun, Guo Yu
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- 2021
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13. Efficacy of Tong-Xie-Yao-Fang granule and its impact on whole transcriptome profiling in diarrhea-predominant irritable bowel syndrome patients: study protocol for a randomized controlled trial
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Wang, Yan, Huang, Yong-quan, Zhu, Shui-lian, Zhang, Chang-rong, Chen, Xin-lin, Hou, Qiu-ke, and Liu, Feng-bin
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- 2020
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14. Modified Sijunzi decoction in the treatment of ulcerative colitis in the remission phase: study protocol for a series of N-of-1 double-blind, randomised controlled trials
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Chen, Yi-ming, Deng, Jie-min, Wen, Yi, Chen, Bin, Hou, Jiang-tao, Peng, Bin, Zhang, Shi-jing, Mi, Hong, Jiang, Qi-long, Wu, Xia-lin, Liu, Feng-bin, and Chen, Xin-lin
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- 2020
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15. Fast-track multidisciplinary treatment versus conventional treatment for colorectal cancer: a multicenter, open-label randomized controlled study
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Li, Jun, Kong, Xiang-Xing, Zhou, Jiao-Jiao, Song, Yong-Mao, Huang, Xue-Feng, Li, Gen-Hai, Ying, Xiao-Jiang, Dai, Xiao-Yu, Lu, Min, Jiang, Kai, Fu, Dong-Liang, Li, Xin-Lin, He, Jin-Jie, Wang, Jian-Wei, Sun, Li-Feng, Xu, Dong, Xu, Jing-Yan, Chen, Min, Tian, Yu, Li, Jing-Song, Yan, Min, Yuan, Ying, and Ding, Ke-Feng
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- 2019
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16. Jin’s three-needle acupuncture technique for chronic fatigue syndrome: a study protocol for a multicentre, randomized, controlled trial
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Lin, Wenjia, Chen, Xin-lin, Chen, Qi, Wen, Junmao, and Chen, Xinghua
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- 2019
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17. Young Sca-1+ bone marrow stem cell-derived exosomes preserve visual function via the miR-150-5p/MEKK3/JNK/c-Jun pathway to reduce M1 microglial polarization.
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Wang, Yuan, Qin, Wan-yun, Wang, Qi, Liu, Xin-na, Li, Xiang-hui, Ye, Xin-qi, Bai, Ying, Zhang, Yan, Liu, Pan, Wang, Xin-lin, Zhou, Yu-hang, Shao, Zheng-bo, and Yuan, Hui-ping
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VISION ,BONE marrow ,EXOSOMES ,MITOGEN-activated protein kinases ,MICROGLIA ,MITOGENS - Abstract
Background: Polarization of microglia, the resident retinal immune cells, plays important roles in mediating both injury and repair responses post-retinal ischemia–reperfusion (I/R) injury, which is one of the main pathological mechanisms behind ganglion cell apoptosis. Aging could perturb microglial balances, resulting in lowered post-I/R retinal repair. Young bone marrow (BM) stem cell antigen 1-positive (Sca-1
+ ) cells have been demonstrated to have higher reparative capabilities post-I/R retinal injury when transplanted into old mice, where they were able to home and differentiate into retinal microglia. Methods: Exosomes were enriched from young Sca-1+ or Sca-1− cells, and injected into the vitreous humor of old mice post-retinal I/R. Bioinformatics analyses, including miRNA sequencing, was used to analyze exosome contents, which was confirmed by RT-qPCR. Western blot was then performed to examine expression levels of inflammatory factors and underlying signaling pathway proteins, while immunofluorescence staining was used to examine the extent of pro-inflammatory M1 microglial polarization. Fluoro-Gold labelling was then utilized to identify viable ganglion cells, while H&E staining was used to examine retinal morphology post-I/R and exosome treatment. Results: Sca-1+ exosome-injected mice yielded better visual functional preservation and lowered inflammatory factors, compared to Sca-1− , at days 1, 3, and 7 days post-I/R. miRNA sequencing found that Sca-1+ exosomes had higher miR-150-5p levels, compared to Sca-1− exosomes, which was confirmed by RT-qPCR. Mechanistic analysis found that miR-150-5p from Sca-1+ exosomes repressed the mitogen-activated protein kinase kinase kinase 3 (MEKK3)/JNK/c-Jun axis, leading to IL-6 and TNF-α downregulation, and subsequently reduced microglial polarization, all of which contributes to reduced ganglion cell apoptosis and preservation of proper retinal morphology. Conclusion: This study elucidates a potential new therapeutic approach for neuroprotection against I/R injury, via delivering miR-150-5p-enriched Sca-1+ exosomes, which targets the miR-150-5p/MEKK3/JNK/c-Jun axis, thereby serving as a cell-free remedy for treating retinal I/R injury and preserving visual functioning. [ABSTRACT FROM AUTHOR]- Published
- 2023
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18. Percutaneous closure versus medical therapy for stroke with patent foramen Ovale: a systematic review and meta-analysis
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Zhang, Xin-Lin, Kang, Li-Na, Wang, Lian, and Xu, Biao
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- 2018
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19. Epilepsy control during an epidemic: emerging approaches and a new management framework
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Shichuo Li, Xin Lin, Deng Chen, Dong Zhou, Ling Liu, Zhen Hong, and Li-na Zhu
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medicine.medical_specialty ,Coronavirus disease 2019 (COVID-19) ,Control (management) ,Epidemic ,Review ,Virus diseases ,World health ,lcsh:RC346-429 ,03 medical and health sciences ,Epilepsy ,0302 clinical medicine ,Disease control ,Political science ,Pandemic ,medicine ,030212 general & internal medicine ,lcsh:Neurology. Diseases of the nervous system ,General Environmental Science ,business.industry ,Public health ,Medical practice ,Public relations ,medicine.disease ,General Earth and Planetary Sciences ,business ,030217 neurology & neurosurgery - Abstract
Epidemics are a big threat to world health. The ongoing pandemic of corona virus disease 2019 (COVID-19) has caused a series of challenges to public health. One such challenge is the management of chronic diseases such as epilepsy during an epidemic event. Studies on this topic are rather limited and the related medical practice is full of uncertainty. Here we review recent development of potential approaches for epilepsy control during an epidemic and propose a new three-level management framework to address these challenges.
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- 2020
20. Identification of two microRNA signatures in whole blood as novel biomarkers for diagnosis of nasopharyngeal carcinoma
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Huan-Xin Lin, Xin Hua, Sai-Lan Liu, Zi-Jian Lu, Qian Zhu, Shi-Juan Mai, Jia-Ling Huang, Zhi-Qing Long, Mei-Yin Zhang, Ling Guo, Hui-Yun Wang, Xiao-Qing Sun, Wen Wen, Qi Yang, and Chao Lin
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0301 basic medicine ,Oncology ,Adult ,Male ,medicine.medical_specialty ,Microarray ,lcsh:Medicine ,medicine.disease_cause ,Diagnostic signature ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Expression profile ,Internal medicine ,microRNA ,medicine ,otorhinolaryngologic diseases ,Biomarkers, Tumor ,Humans ,Circulating MicroRNA ,Early Detection of Cancer ,Whole blood ,Aged ,Nasopharyngeal Carcinoma ,business.industry ,Microrna ,Expression Profile ,Diagnostic Signature ,Research ,Gene Expression Profiling ,lcsh:R ,MicroRNA ,Nasopharyngeal Neoplasms ,General Medicine ,Middle Aged ,medicine.disease ,Microarray Analysis ,Fold change ,Reverse transcription polymerase chain reaction ,Gene Expression Regulation, Neoplastic ,stomatognathic diseases ,MicroRNAs ,030104 developmental biology ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,Case-Control Studies ,Female ,Differential diagnosis ,Carcinogenesis ,business ,Transcriptome - Abstract
Background Early diagnosis is critical to reduce the mortality caused by nasopharyngeal carcinoma (NPC). MicroRNAs (miRNAs) are dysregulated and play important roles in carcinogenesis. Therefore, this study aimed to identify diagnostically relevant circulating miRNA signatures in patients with NPC. Methods Total RNA was extracted from whole blood samples obtained from 120 patients with NPC, 30 patients with head-neck tumors (HNT), and 30 healthy subjects (HSs), and examined by using a custom microarray. The expression levels of four miRNAs identified by using the microarray were validated with quantitative real-time reverse transcription polymerase chain reaction. The 120 patients with NPC and 30 HSs were randomly assigned to training group-1 and validation group-1, respectively. By using significance analysis of microarray (SAM), the specific miRNA expression profiles in whole blood from patients with NPC are obtained. By using lasso regression and adaptive boosting, a diagnostic signature was identified in training group-1, and its accuracy was verified in validation group-1. By using the same methods, another signature to distinguish patients with NPC from those with HNT and HSs was identified in training group-2 and confirmed in validation group-2. Results There were 117 differentially expressed miRNAs (upregulated and downregulated fold change ≥ 1.5) between the patients with NPC and HSs, among which an 8-miRNA signature was identified with 96.43% sensitivity and 100% specificity [area under the curve (AUC) = 0.995] to diagnose NPC in training group-1 and 86.11% sensitivity and 88.89% specificity (AUC = 0.941) in validation group-1. Compared with traditional Epstein–Barr virus (EBV) seromarkers, this signature was more specific for NPC. Furthermore, a 16-miRNA signature to differentiate NPC from HNT and HS (HNT-HS) was established from 164 differentially expressed miRNAs, which diagnosed NPC and HNT-HS with 100% accuracy (AUC = 1.000) in training group-2 and 87.04% (AUC = 0.924) in validation group-2. Conclusions The present study identified two miRNA signatures for the highly accurate diagnosis and differential diagnosis of patients with NPC from HSs and patients with HNT. The identified miRNAs might represent novel serological biomarkers and potential therapeutic targets for NPC. Electronic supplementary material The online version of this article (10.1186/s12967-019-1923-2) contains supplementary material, which is available to authorized users.
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- 2019
21. The diagnostic and prognostic values of plasma Epstein-Barr virus DNA for residual cervical lymphadenopathy in nasopharyngeal carcinoma patients: a retrospective study
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Hu Liang, Li Ting Liu, Xiao Yun Li, Qing Nan Tang, Hai-Qiang Mai, Xue Song Sun, Chao Lin, Jin Jie Yan, Sai Lan Liu, Lin Quan Tang, Shan Shan Guo, Qiu Yan Chen, Yang Li, Ling Guo, Yu Jing Liang, Hao Jun Xie, and Huan Xin Lin
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0301 basic medicine ,Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,Survival ,medicine.medical_treatment ,Lymphadenopathy ,Gastroenterology ,lcsh:RC254-282 ,Fine needle aspiration cytology ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Cervical lymphadenopathy ,Internal medicine ,medicine ,Residual cervical lymphadenopathy ,Epstein-Barr virus ,Humans ,Aged ,Retrospective Studies ,Chemotherapy ,Nasopharyngeal Carcinoma ,Proportional hazards model ,business.industry ,Hazard ratio ,Neck dissection ,Retrospective cohort study ,Nasopharyngeal Neoplasms ,Middle Aged ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Prognosis ,Survival Analysis ,030104 developmental biology ,Real-time polymerase chain reaction ,Oncology ,Nasopharyngeal carcinoma ,030220 oncology & carcinogenesis ,DNA, Viral ,Original Article ,Female ,Lymph Nodes ,medicine.symptom ,business ,Neck - Abstract
Background Currently, the diagnosis and treatment of nasopharyngeal carcinoma (NPC) patients with residual cervical lymphadenopathy following radical radiotherapy with or without chemotherapy are challenging. We investigated the prognosis of NPC patients with residual cervical lymphadenopathy and assessed the diagnostic and prognostic values of Epstein-Barr virus (EBV) DNA in these patients. Methods This study included 82 NPC patients who were diagnosed with suspected residual cervical lymphadenopathy following completion of antitumor therapy. Their plasma EBV DNA levels were measured using quantitative polymerase chain reaction (qPCR) before the initiation of treatment and before neck dissection. Fine needle aspiration cytology (FNAC) was performed in 21 patients. All patients had undergone neck dissection and postoperative pathological examination to identify the nature of residual cervical lymphadenopathy. The overall survival (OS), progression-free survival (PFS), distant metastasis-free survival (DMFS), and locoregional relapse-free survival (LRRFS) were calculated using the Kaplan–Meier method and compared using the log-rank test. The Cox proportional hazards model was used to calculate hazard ratios (HRs) with 95% confidence intervals (CIs). Multivariable analysis was used to estimate the effect of potential prognostic factors on survival. Results Following a median follow-up of 52.6 months, compared with patients with negative postoperative pathological findings for residual cervical lymphadenopathy, the patients with positive findings had a significantly lower 3-year PFS rate (49.9% vs. 83.3%, P = 0.008). Among NPC patients with residual cervical lymphadenopathy, the patients with preoperative plasma EBV DNA > 0 copy/mL had a lower 3-year PFS rate than did those with no detectable EBV DNA (43.7% vs. 61.1%, P = 0.031). In addition, combining FNAC with preoperative EBV DNA detection improved the diagnostic sensitivity. Multivariable analysis demonstrated that residual cervical lymphadenopathy with positive postoperative pathological result was an independent prognostic factor for PFS and that detectable preoperative plasma EBV DNA was an independent prognostic factor for OS. Conclusions Using FNAC combined with preoperative EBV DNA detection improves the sensitivity in diagnosing NPC with residual cervical lymphadenopathy. Compared with patients with undetectable EBV DNA, patients with detectable preoperative plasma EBV DNA have worse prognosis and may require a more aggressive treatment strategy.
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- 2019
22. Liposomal paclitaxel versus docetaxel in induction chemotherapy using Taxanes, cisplatin and 5-fluorouracil for locally advanced nasopharyngeal carcinoma
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Xiao Yun Li, Jin Jie Yan, Xue Song Sun, Shan Shan Guo, Qing Nan Tang, Chao Lin, Yu Jing Liang, Lin Quan Tang, Yue Feng Wen, Hao Jun Xie, Qiu Yan Chen, Ling Guo, Huan Xin Lin, Hai-Qiang Mai, Zhen Chong Yang, Sai Lan Liu, and Li Ting Liu
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0301 basic medicine ,Oncology ,Male ,Cancer Research ,Herpesvirus 4, Human ,Docetaxel ,chemistry.chemical_compound ,0302 clinical medicine ,Nasopharyngeal Carcinoma ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Chemotherapy regimen ,Paclitaxel ,Fluorouracil ,030220 oncology & carcinogenesis ,Induction chemotherapy ,Female ,Taxoids ,medicine.drug ,Research Article ,Adult ,medicine.medical_specialty ,Neutropenia ,Adolescent ,Drug-Related Side Effects and Adverse Reactions ,lcsh:RC254-282 ,Disease-Free Survival ,03 medical and health sciences ,Internal medicine ,Cell Line, Tumor ,Genetics ,medicine ,Humans ,Aged ,Liposomal paclitaxel ,business.industry ,medicine.disease ,Regimen ,030104 developmental biology ,Nasopharyngeal carcinoma ,chemistry ,Liposomes ,Cisplatin ,Neoplasm Recurrence, Local ,business - Abstract
Background We wished to evaluate the efficacy and safety of liposomal paclitaxel and docetaxel for induction chemotherapy (IC) for nasopharyngeal carcinoma (NPC). Methods A total of 1498 patients with newly-diagnosed NPC between 2009 and 2017 treated with IC plus concurrent chemotherapy were included in our observational study. Overall survival (OS), progression-free survival (PFS), locoregional relapse-free survival (LRFS), distant metastasis-free survival (DMFS) and grade-3–4 toxicities were compared between groups using propensity score matching (PSM). Results In total, 767 patients were eligible for this study, with 104 (13.6%) and 663 (86.4%) receiving a liposomal paclitaxel-based and docetaxel-based taxanes, cisplatin and 5-fluorouracil (TPF) regimen, respectively. PSM identified 103 patients in the liposomal-paclitaxel group and 287 patients in the docetaxel group. There was no significant difference at 3 years for OS (92.2% vs. 93.9%, P = 0.942), PFS (82.6% vs. 81.7%, P = 0.394), LRFS (94.7% vs. 93.3%, P = 0.981) or DMFS (84.6% vs. 87.4%, P = 0.371) between the two groups after PSM. Significant interactions were not observed between the effect of chemotherapy regimen and sex, age, T stage, N stage, overall stage, or Epstein–Barr virus DNA level in the subgroup multivariate analysis. The prevalence of grade-3–4 leukopenia and neutropenia in the liposomal-paclitaxel group was significantly lower than that of the docetaxel group (P
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- 2018
23. Cupping therapy versus acupuncture for pain-related conditions: a systematic review of randomized controlled trials and trial sequential analysis
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Bao-Yong Lai, Ya-Jing Zhang, Xiao-Ying Yang, Guo-Yang Yang, Jianping Liu, Xin-Lin Li, and Huijuan Cao
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medicine.medical_specialty ,Trial sequential analysis ,medicine.medical_treatment ,Review ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,Acupuncture ,medicine ,Cervical spondylosis ,VDP::Medisinske Fag: 700 ,030212 general & internal medicine ,Adverse effect ,Pharmacology ,Cupping therapy ,business.industry ,Absolute risk reduction ,lcsh:Other systems of medicine ,lcsh:RZ201-999 ,medicine.disease ,Confidence interval ,VDP::Medical disciplines: 700 ,Complementary and alternative medicine ,Relative risk ,Physical therapy ,Systematic review ,business ,030217 neurology & neurosurgery - Abstract
Source at: https://doi.org/10.1186/s13020-017-0142-0 Background: Both cupping therapy and acupuncture have been used in China for a long time, and their target indications are pain-related conditions. There is no systematic review comparing the effectiveness of these two therapies. Objectives: To compare the beneficial effectiveness and safety between cupping therapy and acupuncture for pain-related conditions to provide evidence for clinical practice. Methods: Protocol of this review was registered in PROSPERO (CRD42016050986). We conducted literature search from six electronic databases until 31st March 2017. We included randomized trials comparing cupping therapy with acupuncture on pain-related conditions. Methodological quality of the included studies was evaluated by risk of bias tool. Mean difference, risk ratio, risk difference and their 95% confidence interval were used to report the estimate effect of the pooled results through meta-analysis or the results from each individual study. Trial sequential analysis (TSA) was applied to adjust random errors and calculate the sample size. Results: Twenty-three randomized trials with 2845 participants were included covering 12 pain-related conditions. All included studies were of poor methodological quality. Three meta-analyses were conducted, which showed similar clinical beneficial effects of cupping therapy and acupuncture for the rate of symptom improvement in cervical spondylosis (RR 1.13, 95% CI 1.01 to 1.26; n = 646), lateral femoral cutaneous neuritis (RR 1.10, 95% CI 1.00 to 1.22; n = 102) and scapulohumeral periarthritis (RR 1.31, 95% CI 1.15 to 1.51; n = 208). Results from other outcomes (such as visual analogue and numerical rating scale) in each study also showed no statistical significant difference between these two therapies for all included pain-related conditions. The results of TSA for cervical spondylosis demonstrated that the current available data have not reached a powerful conclusion. No serious adverse events related to cupping therapy or acupuncture was found in included studies. Conclusion: Cupping therapy and acupuncture are potentially safe, and they have similar effectiveness in relieving pain. However, further rigorous studies investigating relevant pain-related conditions are warranted to establish comparative effectiveness analysis between these two therapies. Cost-effectiveness studies should be considered in the future studies to establish evidence for decision-making in clinical practice.
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- 2017
24. Epidemiological investigation of Mycoplasma Synoviae in native chicken breeds in China
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Shikai Sun, Ding-Ai Wang, Feng Chen, Xin Lin, Jianping Qin, Tingrong Luo, and Junpeng Lu
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0301 basic medicine ,musculoskeletal diseases ,medicine.medical_specialty ,Veterinary medicine ,China ,animal structures ,040301 veterinary sciences ,Epidemiology ,Antibody level ,Mycoplasma synoviae ,Biology ,Polymerase Chain Reaction ,Chinese native chicken breeds ,Disease Outbreaks ,0403 veterinary science ,03 medical and health sciences ,fluids and secretions ,Species Specificity ,medicine ,Prevalence ,Animals ,Mycoplasma Infections ,skin and connective tissue diseases ,Poultry Diseases ,lcsh:Veterinary medicine ,General Veterinary ,Incidence (epidemiology) ,Broiler ,Outbreak ,04 agricultural and veterinary sciences ,General Medicine ,Sequence Analysis, DNA ,musculoskeletal system ,Virology ,030104 developmental biology ,lcsh:SF600-1100 ,Flock ,Chickens ,Horizontal transmission ,Research Article - Abstract
Backgroud Mycoplasma synoviae (M. synoviae) is widely distributed around the world, and leads to serious economic losses in the world every year. Nevertheless, the incidence and epidemiology of M. synoviae infection in China have remained unclear. Results In this study we demonstrate that over 9773 broiler chicken flocks in 16 Chinese provinces were affected by M. synoviae between 2010 and 2015. Our epidemiological study revealed that M. synoviae was widely prevalent in multi-aged Chinese native breeder chickens, and the prevalence of M. synoviae in embryos of breeders reached up to 16.29%. In addition, our data showed that chickens aged 14 days or younger carried simultaneously high levels of maternal antibody against M. synoviae and high M. synoviae infection (10%), and low M. synoviae antibody levels in breeders and high proportion of M. synoviae infection in embryos could increase the chances of incidence in the offspring. Finally, our results also indicated that 3- to 7-week-old chickens might be most the susceptible to M. synoviae and, therefore, might play a key role in the horizontal transmission of M. synoviae. Conclusion Our findings suggest that M. synoviae is widely circulating in Chinese native chickens, accordingly, effective control measures are urgently needed to control the spread.
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- 2017
25. Clinical diagnostic value of viable Schistosoma japonicum eggs detected in host tissues
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Kongzhen Gu, Qingren Zeng, Xin-lin Yu, Yuesheng Li, Patrick Driguez, Liting Cai, Wenyang Wang, Hui Sun, Donald P. McManus, and Yong-Kang He
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0301 basic medicine ,Sdhg ,Male ,Pathology ,Biopsy ,Praziquantel ,Schistosoma japonicum ,Mice ,0302 clinical medicine ,Medical microbiology ,Intestinal Mucosa ,biology ,medicine.diagnostic_test ,Morphological characteristics of eggs ,Infectious Diseases ,Liver ,Schistosomiasis japonica ,Alkaline phosphatase ,Female ,medicine.drug ,Research Article ,SjR2mRNA ,medicine.medical_specialty ,Colon ,030231 tropical medicine ,Schistosomiasis ,Microbiology ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,In vivo ,medicine ,Aos ,Animals ,Humans ,lcsh:RC109-216 ,RNA, Messenger ,CalS ,Ovum ,Rectum ,Reproducibility of Results ,biology.organism_classification ,medicine.disease ,Vitality of eggs ,030104 developmental biology ,Parasitology ,Alp ,RNA, Helminth ,Biomarkers - Abstract
Background Schistosomiasis, one of the neglected tropical diseases, is endemic in more than 70 countries. However, the clinical diagnosis of patients with a low degree of infection is an unsolved technical problem. In areas endemic for schistosomiasis japonica, proctoscopy detection of eggs has been one method used for clinical diagnosis. However, it is often a challenge to find typical live eggs and it is difficult to distinguish live eggs from large numbers of partially degraded and/or completely degraded eggs within colon biopsy tissue. To address this problem, we tested six different morphological and biochemical/molecular markers (ALP; morphological characteristics of egg; CalS (calcified substance); AOS (antioxidase); SDHG (succinic dehydrogenase) and SjR2 mRNA (retrotransposons 2 of S.japonicum genome mRNA)), including four new markers (CalS; AOS; SDHG and SjR2 mRNA.), to determine the viability of S. japonicum eggs deposited in human and mouse colon tissues. Our ultimate aim is to obtain a new method that is more sensitive, practical and accurate to clinically diagnose schistosomiasis. Methods Tissue samples were collected from mice at six different time points during S. japonicum infection with or without treatment with praziquantel (PZQ). Four new biochemical or molecular markers were used for the detection of egg viability from mouse liver and intestinal samples: CalS; AOS; SDHG and SjR2 mRNA. Subsequently, all markers were employed for the detection and analysis of eggs deposited in biopsy materials from patients with suspected schistosomiasis japonica for clinical evaluation. Microscopic examination of the egg morphology, worm burden in vivo and ALP (alkaline phosphatase) levels were used as a reference standard to evaluate the sensitivity and reliability of four new markers detecting egg viability. Results The results of the study showed that the morphology of S. japonicum eggs deposited in tissues of hosts with schistosomiasis, especially cases with chronic schistosomiasis, is complex and egg viability is difficult to judge morphologically, particularly eggs with a fuzzy structure or partially modified eggs. We found that the majority of the viable schistosome eggs determined by four new markers (CalS, AOS, SDHG and SjR2 mRNA) were morphologically difficult to identify. Conclusions Among the markers, the most sensitive and specific method was the detection of SjR2 mRNA and the most simple, rapid and practical method was the detection of SDHG. Therefore, the detection of SDHG is the most practical for clinical application and its use could improve the accuracy in diagnosing active schistosome infection. Electronic supplementary material The online version of this article (doi:10.1186/s12879-017-2362-4) contains supplementary material, which is available to authorized users.
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- 2017
26. Thymosin beta 10 is a key regulator of tumorigenesis and metastasis and a novel serum marker in breast cancer
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Shu Wu, Libing Song, Lan Wang, Huan-Xin Lin, Chuyong Lin, Liping Ye, Xin Zhang, Zhen-Yu He, Jinrong Zhu, Ling Guo, Jun Li, and Dong Ren
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0301 basic medicine ,CA15-3 ,Oncology ,Carcinogenesis ,Proliferation ,Kaplan-Meier Estimate ,medicine.disease_cause ,Metastasis ,chemistry.chemical_compound ,0302 clinical medicine ,Breast cancer ,Surgical oncology ,Cell Movement ,Neoplasm Metastasis ,skin and connective tissue diseases ,Serum marker ,Medicine(all) ,AKT/FOXO signaling ,Forkhead Transcription Factors ,Perifosine ,Prognosis ,030220 oncology & carcinogenesis ,Female ,Research Article ,Signal Transduction ,medicine.medical_specialty ,CA 15-3 ,Breast Neoplasms ,Cell cycle ,03 medical and health sciences ,Internal medicine ,Cell Line, Tumor ,medicine ,Biomarkers, Tumor ,Humans ,Cell Proliferation ,Proportional Hazards Models ,TMSB10 ,business.industry ,Cancer ,medicine.disease ,Thymosin ,030104 developmental biology ,chemistry ,Tumorigenesis ,business ,Proto-Oncogene Proteins c-akt - Abstract
Background Thymosin beta 10 (TMSB10) has been demonstrated to be involved in the malignant process of many cancers. The purpose of this study was to determine the biological roles and clinical significance of TMSB10 in breast cancer and to identify whether TMSB10 might be used as a serum marker for the diagnosis of breast cancer. Methods TMSB10 expression was evaluated by immunohistochemical analysis (IHC) of 253 breast tumors and ELISA of serum from 80 patients with breast cancer. Statistical analysis was performed to explore the correlation between TMSB10 expression and clinicopathological features in breast cancer. Univariate and multivariate Cox regression analysis were performed to examine the association between TMSB10 expression and overall survival and metastatic status. In vitro and in vivo assays were performed to assess the biological roles of TMSB10 in breast cancer. Western blotting and luciferase assays were examined to identify the underlying pathway involved in the tumor-promoting role of TMSB10. Results We found TMSB10 was upregulated in breast cancer cells and tissues. Univariate and multivariate analysis demonstrated that high TMSB10 expression significantly correlated with clinicopathological features, poor prognosis and distant metastases in patients with breast cancer. Overexpression of TMSB10 promotes, while silencing of TMSB10 inhibits, proliferation, invasion and migration of breast cancer cells in vitro and in vivo. Our results further reveal that TMSB10 promotes the proliferation, invasion and migration of breast cancer cells via AKT/FOXO signaling, which is antagonized by the AKT kinase inhibitor perifosine. Importantly, the expression of TMSB10 is significantly elevated in the serum of patients with breast cancer and is positively associated with clinical stages of breast cancer. Conclusion TMSB10 may hold promise as a minimally invasive serum cancer biomarker for the diagnosis of breast cancer and a potential therapeutic target which will facilitate the development of a novel therapeutic strategy against breast cancer. Electronic supplementary material The online version of this article (doi:10.1186/s13058-016-0785-2) contains supplementary material, which is available to authorized users.
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- 2017
27. Inflammatory bowel disease-specific health-related quality of life instruments: a systematic review of measurement properties.
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Xin-Lin Chen, Liang-huan Zhong, Yi Wen, Tian-Wen Liu, Xiao-Ying Li, Zheng-Kun Hou, Yue Hu, Chuan-wei Mo, Feng-Bin Liu, Chen, Xin-Lin, Zhong, Liang-Huan, Wen, Yi, Liu, Tian-Wen, Li, Xiao-Ying, Hou, Zheng-Kun, Hu, Yue, Mo, Chuan-Wei, and Liu, Feng-Bin
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INFLAMMATORY bowel diseases , *INFLAMMATORY bowel disease treatment , *QUALITY of life , *MEDICAL care , *TREATMENT effectiveness , *PATIENTS , *RESEARCH methodology , *SYSTEMATIC reviews , *PSYCHOLOGY ,RESEARCH evaluation - Abstract
Background: This review aims to critically appraise and compare the measurement properties of inflammatory bowel disease (IBD)-specific health-related quality of life instruments.Methods: Medline, EMBASE and ISI Web of Knowledge were searched from their inception to May 2016. IBD-specific instruments for patients with Crohn's disease, ulcerative colitis or IBD were enrolled. The basic characteristics and domains of the instruments were collected. The methodological quality of measurement properties and measurement properties of the instruments were assessed.Results: Fifteen IBD-specific instruments were included, which included twelve instruments for adult IBD patients and three for paediatric IBD patients. All of the instruments were developed in North American and European countries. The following common domains were identified: IBD-related symptoms, physical, emotional and social domain. The methodological quality was satisfactory for content validity; fair in internal consistency, reliability, structural validity, hypotheses testing and criterion validity; and poor in measurement error, cross-cultural validity and responsiveness. For adult IBD patients, the IBDQ-32 and its short version (SIBDQ) had good measurement properties and were the most widely used worldwide. For paediatric IBD patients, the IMPACT-III had good measurement properties and had more translated versions.Conclusions: Most methodological quality should be promoted, especially measurement error, cross-cultural validity and responsiveness. The IBDQ-32 was the most widely used instrument with good reliability and validity, followed by the SIBDQ and IMPACT-III. Further validation studies are necessary to support the use of other instruments. [ABSTRACT FROM AUTHOR]- Published
- 2017
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28. Prognostic value of transforming growth factor-beta in patients with colorectal cancer who undergo surgery: a meta-analysis.
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Xin-lin Chen, Zhuo-qun Chen, Shui-lian Zhu, Tian-wen Liu, Yi Wen, Yi-sheng Su, Xu-jie Xi, Yue Hu, Lei Lian, Feng-bin Liu, Chen, Xin-Lin, Chen, Zhuo-Qun, Zhu, Shui-Lian, Liu, Tian-Wen, Wen, Yi, Su, Yi-Sheng, Xi, Xu-Jie, Hu, Yue, Lian, Lei, and Liu, Feng-Bin
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COLON cancer treatment , *TRANSFORMING growth factors-beta , *COLON cancer patients , *ONCOLOGIC surgery , *CONFIDENCE intervals , *THERAPEUTICS , *ASIANS , *COLON tumors , *COMPARATIVE studies , *GROWTH factors , *RESEARCH methodology , *MEDICAL cooperation , *META-analysis , *PROGNOSIS , *RESEARCH , *EVALUATION research , *PROPORTIONAL hazards models ,RECTUM tumors - Abstract
Background: Transforming growth factor-beta (TGF-β) is associated with a higher incidence of distant metastasis and decreased survival. Whether TGF-β can be used as a prognostic indicator of colorectal cancer (CRC) remains controversial.Methods: The Medline, EMBASE and Cochrane databases were searched from their inception to March 2016. The studies that focused on TGF-β as a prognostic factor in patients with CRC were included in this analysis. Overall survival (OS) and disease-free survival (DFS) were analysed separately. A meta-analysis was performed, and hazard ratios (HR) with 95% confidence intervals (CI) were calculated.Results: Twelve studies were included in the analysis, of which 8 were used for OS and 7 for DFS. In all, 1622 patients with CRC undergoing surgery were included. Combined HRs suggested that high expression of TGF-β had a favourable impact on OS (HR = 1.68, 95% CI: 1.10-2.59) and DFS (HR = 1.11, 95% CI: 1.03-1.19) in CRC patients. For OS, the combined HRs of Asian studies and Western studies were 1.50 (95% CI: 0.61-3.68) and 1.80 (95% CI: 1.33-2.45), respectively. For DFS, the combined HRs of Asian studies and Western studies were 1.42 (95% CI: 0.61-3.31) and 1.11 (95% CI: 1.03-1.20), respectively.Conclusions: This meta-analysis demonstrates that TGF-β can be used as a prognostic biomarker for CRC patients undergoing surgery, especially for CRC patients from Western countries. [ABSTRACT FROM AUTHOR]- Published
- 2017
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29. Small intestinal submucosa-derived extracellular matrix bioscaffold significantly enhances angiogenic factor secretion from human mesenchymal stromal cells
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Tye Petrie, Veronica Spandler, Claus S. Sondergaard, Xin Lin, W. Douglas Boyd, and Mikella Robinson
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medicine.medical_treatment ,Medicine (miscellaneous) ,Biology ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Extracellular matrix ,Paracrine signalling ,chemistry.chemical_compound ,Intestinal mucosa ,medicine ,Humans ,Viability assay ,Intestinal Mucosa ,Cells, Cultured ,Tissue Scaffolds ,Research ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Cell Biology ,Cell biology ,Extracellular Matrix ,Vascular endothelial growth factor ,Cytokine ,chemistry ,Culture Media, Conditioned ,Immunology ,Molecular Medicine ,Angiogenesis Inducing Agents ,Stem cell - Abstract
Introduction The in vivo therapeutic effect of mesenchymal stromal cells (MSCs) is currently believed to be tightly linked to their paracrine secretion ability. However, insufficient or imprecise cell delivery, low cell survival and retention post-transplant, along with harsh donor site microenvironments, are major barriers to the clinical success of MSC therapies. Here we tested a small intestinal submucosa (SIS)-derived extracellular matrix (ECM) bioscaffold augmented with MSCs, with the hypothesis that they will facilitate the precise delivery of increased numbers of MSCs therefore improving cell viability and retention. Methods In this study, we evaluated the secretion of angiogenic factors from three human MSC lines cultured on SIS ECM. We used human antibody array and enzyme-linked immunosorbent assay to measure the level of angiogenic factors released from MSCs when cultured on SIS ECM or regular tissue culture plastic. We tested MSCs cultured for three different time points. Results We found that the SIS ECM culture environment can significantly enhance the release of several angiogenic factors when compared to MSCs cultured on standard tissue culture plastic. Specifically, vascular endothelial growth factor and interleukin-8 secretion was significantly increased at 24, 48 and 72 hours postseeding onto SIS ECM whereas vascular endothelial growth factor release for cells cultured on plastic surface remained the same during these time points. We also observed significant donor to donor variation in cytokine production. Conclusions This study demonstrates that MSCs transplanted onto a SIS ECM may greatly increase their therapeutic potential through an increase in pro-angiogenic cytokine release.
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- 2015
30. Development and validation of quality of life scale of nasopharyngeal carcinoma patients: the QOL-NPC (version 2).
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Yong Su, Chuan-wei Mo, Wan-qin Cheng, Lei Wang, Qian Xu, Zu-chun Wu, Zhe-li Wu, Li-zhi Liu, Xin-lin Chen, Su, Yong, Mo, Chuan-Wei, Cheng, Wan-Qin, Wang, Lei, Xu, Qian, Wu, Zu-Chun, Wu, Zhe-Li, Liu, Li-Zhi, and Chen, Xin-Lin
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QUALITY of life ,NASOPHARYNX cancer patients ,RADIOTHERAPY ,CLASSICAL test theory ,CRONBACH'S alpha ,STANDARD deviations ,CONFIDENCE intervals ,MENTAL health ,NASOPHARYNX tumors ,PSYCHOMETRICS ,COMPARATIVE studies ,RESEARCH methodology ,MEDICAL cooperation ,PATIENT satisfaction ,PATIENT psychology ,RESEARCH ,RESEARCH evaluation ,ASIANS ,SOCIOECONOMIC factors ,EVALUATION research ,HUMAN services programs ,PSYCHOLOGY ,TUMOR treatment ,EQUIPMENT & supplies - Abstract
Background: The aim was to develop and validate the quality of life scale for nasopharyngeal carcinoma (NPC) patients, the QOL-NPC (version 2), a specific instrument to measure quality of life for NPC patients.Methods: The QOL-NPC was developed and validated according to standard procedures. The patients were assessed using the QOL-NPC, FACT-G, and FACT-H&N. Classical test theory was used to evaluate the reliability, validity, and responsiveness of the QOL-NPC.Results: A total of 487 patients (97.4 %) completed the questionnaire. The QOL-NPC comprised four domains, as follows: physical function (eight items); psychological function (five items); social function (five items); and side effects (eight items). All of the items had a lower proportion of missing data. Cronbach's alpha values of the domains ranged from 0.72 to 0.84. The split-half reliability coefficients ranged from 0.77 to 0.84. All of the intra-class correlation coefficients were > 0.8. The normed fit index, non-normed fit index, and comparative fit index were >0.89. The root mean square error of approximation was 0.097, with a 90 % confidence interval (0.093, 0.100). The domain scores of the QOL-NPC were significantly correlated with the FACT-G and FACT-H&N (P < 0.05). All of the domain scores of patients using different amounts of radiotherapy were significantly different (P < 0.001). All domain scores decreased at the completion of radiotherapy, with effect sizes ranging from -0.82 to -0.22.Conclusions: The QOL-NPC is valid for measuring QOL with good reliability, validity, and responsiveness. The QOL-NPC is recommended to measure the QOL for Chinese NPC patients. [ABSTRACT FROM AUTHOR]- Published
- 2016
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31. Efficient induction of functional ameloblasts from human keratinocyte stem cells.
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Xuefeng Hu, Jyh-Wei Lee, Xi Zheng, Junhua Zhang, Xin Lin, Yingnan Song, Bingmei Wang, Xiaoxiao Hu, Hao-Hueng Chang, Yiping Chen, Chun-Pin Lin, and Yanding Zhang
- Abstract
Background: Although adult human tissue-derived epidermal stem cells are capable of differentiating into enamel-secreting ameloblasts and forming teeth with regenerated enamel when recombined with mouse dental mesenchyme that possesses odontogenic potential, the induction rate is relatively low. In addition, whether the regenerated enamel retains a running pattern of prism identical to and acquires mechanical properties comparable with human enamel indeed warrants further study. Methods: Cultured human keratinocyte stem cells (hKSCs) were treated with fibroblast growth factor 8 (FGF8) and Sonic hedgehog (SHH) for 18 h or 36 h prior to being recombined with E13.5 mouse dental mesenchyme with implantation of FGF8 and SHH-soaked agarose beads into reconstructed chimeric tooth germs. Recombinant tooth germs were subjected to kidney capsule culture in nude mice. Harvested samples at various time points were processed for histological, immunohistochemical, TUNEL, and western blot analysis. Scanning electronic microscopy and a nanoindentation test were further employed to analyze the prism running pattern and mechanical properties of the regenerated enamel. Results: Treatment of hKSCs with both FGF8 and SHH prior to tissue recombination greatly enhanced the rate of tooth-like structure formation to about 70%. FGF8 and SHH dramatically enhanced stemness of cultured hKSCs. Scanning electron microscopic analysis revealed the running pattern of intact prisms of regenerated enamel is similar to that of human enamel. The nanoindentation test indicated that, although much softer than human child and adult mouse enamel, mechanical properties of the regenerated enamel improved as the culture time was extended. Conclusions: Application of FGF8 and SHH proteins in cultured hKSCs improves stemness but does not facilitate odontogenic fate of hKSCs, resulting in an enhanced efficiency of ameloblastic differentiation of hKSCs and tooth formation in human–mouse chimeric tooth germs. [ABSTRACT FROM AUTHOR]
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- 2018
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32. Thymosin β4 alleviates renal fibrosis and tubular cell apoptosis through TGF-β pathway inhibition in UUO rat models.
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Jing Yuan, Yan Shen, Xia Yang, Ying Xie, Xin Lin, Wen Zeng, Yingting Zhao, Maolu Tian, Yan Zha, Yuan, Jing, Shen, Yan, Yang, Xia, Xie, Ying, Lin, Xin, Zeng, Wen, Zhao, Yingting, Tian, Maolu, and Zha, Yan
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THYMOSIN ,RENAL fibrosis ,CYTOSKELETON ,NEOVASCULARIZATION ,APOPTOSIS ,TRANSFORMING growth factors ,EPITHELIAL cells ,RNA metabolism ,MUSCLE protein metabolism ,ANIMAL experimentation ,BIOLOGICAL models ,CELL culture ,CELLULAR signal transduction ,GLYCOPROTEINS ,GROWTH factors ,KIDNEY tubules ,KIDNEYS ,MUSCLE proteins ,PEPTIDE hormones ,PROTEINURIA ,RATS ,URETERIC obstruction ,FIBROSIS ,DISEASE complications - Abstract
Background: Thymosin β4 (Tβ4) is closely associated with the cytoskeleton, inflammation, wound healing, angiogenesis, apoptosis, and myocardial regeneration, but the effects of Tβ4 treatment on chronic renal tubular interstitial fibrosis (CRTIF) are poorly known. This study aimed to examine the effects of Tβ4 on the renal apoptosis and the expression of transforming growth factor (TGF-β), E-cadherin, and α-smooth muscle actin (α-SMA) in CRTIF rat models.Methods: Male SD rats were randomized into four groups (sham group, unilateral ureteral obstruction (UUO) group, UUO + low-dose Tβ4 group, and UUO + high-dose Tβ4 group). The pathological changes of kidney tissue and its function were assessed two weeks after UUO. In renal interstitial tissue,TGF-β, E-cadherin and α-SMA expression was detected by western blot. In tubular epithelial cells, E-cadherin and α-SMA expression was detected using Real-time qPCR and western blot. Cell apoptosis of rat renal interstitial tissue and tubular epithelial cells was evaluated by immunofluorescence and western blot.Results: Two weeks after UUO, no differences in blood urea nitrogen and creatinine were observed between the four groups (P > 0.05). Compared to the UUO group, Tβ4 treatment decreased the 24-h proteinuria (P < 0.001) and reduced the area of pathological change (P < 0.01); this effect was more apparent in the UUO + high-dose Tβ4 group. Compared to the UUO group, a significant decrease in TGF-β and α-SMA protein expression was observed in the high-dose Tβ4 group. The level of E-cadherin protein was lower in the UUO group than the Tβ4 groups, and high-dose Tβ4 treatment further increased E-cadherin expression and improved cell apoptosis in the renal interstitial tissue. Analysis of in vitro tubular epithelial cells showed that α-SMA mRNA and protein expression decreased, while E-cadherin mRNA and protein expression increased by Tβ4 treatment. Similarly, these changes were more significant in the UUO + high-dose Tβ4 group. Tβ4 treatment improved the apoptosis of In vitro tubular epithelial cells compared with pure TGF-β stimulation, and equally, the decrease of apoptosis was more apparent in the TGF-β + high-dose Tβ4 group.Conclusions: Tβ4 treatment might alleviate the renal fibrosis and apoptosis of tubular epithelial cells through TGF-β pathway inhibition in UUO rats with CRTIF. [ABSTRACT FROM AUTHOR]- Published
- 2017
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33. Computational and experimental assessment of influences of hemodynamic shear stress on carotid plaque.
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Hui Zhou, Long Meng, Wei Zhou, Lin Xin, Xiangxiang Xia, Shuai Li, Hairong Zheng, Lili Niu, Zhou, Hui, Meng, Long, Zhou, Wei, Xin, Lin, Xia, Xiangxiang, Li, Shuai, Zheng, Hairong, and Niu, Lili
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SHEARING force ,HEMODYNAMICS ,ATHEROSCLEROSIS ,CAROTID artery stenosis ,PARTICLE image velocimetry ,COMPUTATIONAL fluid dynamics ,BIOLOGICAL models ,BIOMECHANICS ,IMAGING phantoms ,PHYSIOLOGIC strain - Abstract
Background: Studies have identified hemodynamic shear stress as an important determinant of endothelial function and atherosclerosis. In this study, we assess the influences of hemodynamic shear stress on carotid plaques.Methods: Carotid stenosis phantoms with three severity (30, 50, 70%) were made from 10% polyvinyl alcohol (PVA) cryogel. The phantoms were placed in a pulsatile flow loop with the same systolic/diastolic phase (35/65) and inlet flow rate (16 L/h). Ultrasonic particle imaging velocimetry (Echo PIV) and computational fluid dynamics (CFD) were used to calculate the velocity profile and shear stress distribution in the carotid stenosis phantoms. Inlet/outlet boundary conditions used in CFD were extracted from Echo PIV experiments to make sure that the results were comparable.Results: Echo PIV and CFD results showed that velocity was largest in 70% than those in 30 and 50% at peak systole. Echo PIV results indicated that shear stress was larger in the upper wall and the surface of plaque than in the center of vessel. CFD results demonstrated that wall shear stress in the upstream was larger than in downstream of plaque. There was no significant difference in average velocity obtained by CFD and Echo PIV in 30% (p = 0.25). Velocities measured by CFD in 50% (93.01 cm/s) and in 70% (115.07 cm/s) were larger than those by Echo PIV in 50% (60.26 ± 5.36 cm/s) and in 70% (89.11 ± 7.21 cm/s).Conclusions: The results suggested that Echo PIV and CFD could obtain hemodynamic shear stress on carotid plaques. Higher WSS occurred in narrower arteries, and the shoulder of plaque bore higher WSS than in bottom part. [ABSTRACT FROM AUTHOR]- Published
- 2017
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34. Cupping therapy versus acupuncture for pain-related conditions: a systematic review of randomized controlled trials and trial sequential analysis.
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Ya-Jing Zhang, Hui-Juan Cao, Xin-Lin Li, Xiao-Ying Yang, Bao-Yong Lai, Guo-Yang Yang, and Jian-Ping Liu
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PAIN management ,ACUPUNCTURE ,ALTERNATIVE medicine ,COMPARATIVE studies ,SYSTEMATIC reviews ,RANDOMIZED controlled trials - Abstract
Copyright of Chinese Medicine is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2017
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35. Anatomic comparison of veins of Labbé between autopsy, digital subtraction angiography and computed tomographic venography.
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Qiong Fang, Anhong Jiang, Wei Tao, Lin Xin, Fang, Qiong, Jiang, Anhong, Tao, Wei, and Xin, Lin
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VEINS ,AUTOPSY ,DIGITAL subtraction angiography ,COMPUTED tomography ,VENOGRAPHY ,CRANIAL sinuses ,COMPARATIVE studies ,RESEARCH methodology ,MEDICAL cooperation ,RESEARCH ,EVALUATION research ,ANATOMY - Abstract
Objective: The drainage portion of the vein of Labbé varies, and it is difficult to predict whether the operation is likely to damage this vein. The aim of this study was to correlate the microanatomy of the vein of Labbé with digital subtraction angiography (DSA) and computed tomographic venography (CTV), in order to provide a basis for the preservation of the vein of Labbé during a supratentorial surgical approach.Methods: A total of 30 human cadavers (60 sides) and 61 living patients (110 sides) were examined in this study. Each cadaver head was injected with blue latex via the superior sagittal sinus and the internal jugular veins. The venograms of each patient were obtained from the venous phases of DSA (60 sides for 36 patients) or CTV (50 sides for 25 patients).Results: The patients were divided into four subgroups based on the location where a vein entered the dural sinus: the transverse sinus group, the tentorial group, the petrosal group, and the upper-transverse sinus group. The veins of Labbé in transverse sinus group and petrosal group directly entered dural sinus. The veins of Labbé in tentorial group and upper-transverse sinus group indirectly entered transverse sinus via the tentorium sinus or the upper-transverse sinus. These sinuses were meningeal veins running through two layers of the cerebral dura mater. The length of meningeal veins in these groups was 10.0 ± 7.2 mm. The veins of Labbé were mainly localized around the STP junction, which was the confluence of sigmoid sinus, transverse sinus, and superior petrosal sinus. The distance between the dural entrance of veins and the STP junction was 16.8 ± 10.2 mm. There was no significant difference in the results of the DSA and CTV examinations when compared to the observations in cadavers.Conclusions: Preoperative venograms are useful to design an individualized surgical approach for the preservation of the vein of Labbé. In general, the supratentorial median approach has the least chance to damage this vein. However, when preoperative venograms show that the vein of Labbé is too close to the confluence of sinuses or the meningeal veins are too long, an alternative approach should be chosen. [ABSTRACT FROM AUTHOR]- Published
- 2017
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36. Epidemiological investigation of Mycoplasma Synoviae in native chicken breeds in China.
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Shi-Kai Sun, Xin Lin, Feng Chen, Ding-Ai Wang, Jun-Peng Lu, Jian-Ping Qin, and Ting-Rong Luo
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MYCOPLASMA synoviae , *CHICKEN diseases , *PANDEMICS , *CHICKEN breeds , *EPIDEMIOLOGY , *INFECTION - Abstract
Backgroud: Mycoplasma synoviae (M. synoviae) is widely distributed around the world, and leads to serious economic losses in the world every year. Nevertheless, the incidence and epidemiology of M. synoviae infection in China have remained unclear. Results: In this study we demonstrate that over 9773 broiler chicken flocks in 16 Chinese provinces were affected by M. synoviae between 2010 and 2015. Our epidemiological study revealed that M. synoviae was widely prevalent in multi-aged Chinese native breeder chickens, and the prevalence of M. synoviae in embryos of breeders reached up to 16.29%. In addition, our data showed that chickens aged 14 days or younger carried simultaneously high levels of maternal antibody against M. synoviae and high M. synoviae infection (10%), and low M. synoviae antibody levels in breeders and high proportion of M. synoviae infection in embryos could increase the chances of incidence in the offspring. Finally, our results also indicated that 3- to 7-week-old chickens might be most the susceptible to M. synoviae and, therefore, might play a key role in the horizontal transmission of M. synoviae. Conclusion: Our findings suggest that M. synoviae is widely circulating in Chinese native chickens, accordingly, effective control measures are urgently needed to control the spread. [ABSTRACT FROM AUTHOR]
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- 2017
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37. Percutaneous intervention versus coronary artery bypass graft surgery in left main coronary artery stenosis: a systematic review and meta-analysis.
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Xin-Lin Zhang, Qing-Qing Zhu, Jing-Jing Yang, Yu-Han Chen, Yang Li, Su-Hui Zhu, Jun Xie, Lian Wang, Li-Na Kang, and Biao Xu
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Background: The optimal revascularization technique in patients with left main coronary artery disease (CAD) remains controversial. We aimed to compare the long-term performance of percutaneous coronary intervention (PCI) versus coronary artery bypass graft (CABG) surgery in treatment of left main CAD. Methods: PubMed, EMBASE, and the Cochrane Library were searched until November 16, 2016. Results: Six randomized controlled trials and 22 matched observational studies including 22,487 patients and 90,167 patient-years of follow-up were included. PCI was associated with an overall higher risk for the major adverse cardiac and cerebrovascular events (hazard ratio (HR), 1.42; 95% confidence interval (CI), 1.14-1.77), mainly driven by higher rates of myocardial infarction (HR, 1.69; 95% CI, 1.22-2.34) and revascularization (HR, 2.80; 95% CI, 1.86-4.22). The overall risks for all-cause death (HR, 1.05; 95% CI, 0.93-1.20), cardiac death (HR, 1.05; 95% CI, 0.69-1.59), stroke (HR, 0.64; 95% CI, 0.33-1.24), and the composite safety endpoint of death, myocardial infarction, or stroke (HR, 1.06; 95% CI, 0.97-1.16) were similar between PCI and CABG. Stratified analysis based on stent types showed that the increased risk for myocardial infarction associated with PCI was only evident in patients with bare-metal stents or early-generation drug-eluting stents (DES), but not newer-generation DES. Stratified analyses based on study designs showed largely similar findings with the overall analyses, except for a significantly higher incidence of myocardial infarction in adjusted studies (HR, 2.01; 95% CI, 1.64-2.45) but a trend toward higher incidence in randomized trials (HR, 1.39; 95% CI, 0.85-2.27) associated with PCI. Conclusions: Compared with CABG, PCI with newer-generation DES might be a safe alternative revascularization strategy for treatment of left main CAD, but is associated with more repeat revascularization. [ABSTRACT FROM AUTHOR]
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- 2017
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38. Applicability of visceral adiposity index in predicting metabolic syndrome in adults with obstructive sleep apnea: a cross-sectional study.
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Gong-Ping Chen, Jia-Chao Qi, Bi-Ying Wang, Xin Lin, Xiao-Bin Zhang, Jian-Ming Zhao, Xiao Fang Chen, Ting Lin, Dong-Dong Chen, Qi-Chang Lin, Chen, Gong-Ping, Qi, Jia-Chao, Wang, Bi-Ying, Lin, Xin, Zhang, Xiao-Bin, Zhao, Jian-Ming, Chen, Xiao Fang, Lin, Ting, Chen, Dong-Dong, and Lin, Qi-Chang
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SLEEP apnea syndromes ,HYPNAGOGIA ,METABOLIC syndrome ,POLYSOMNOGRAPHY ,METABOLIC disorders ,ADIPOSE tissues ,HIGH density lipoproteins ,LONGITUDINAL method ,OBESITY ,TRIGLYCERIDES ,LOGISTIC regression analysis ,BODY mass index ,CROSS-sectional method ,RETROSPECTIVE studies ,SEVERITY of illness index ,RECEIVER operating characteristic curves ,WAIST circumference - Abstract
Background: Obstructive sleep apnea (OSA) is severely affected by visceral adiposity (VA) that correlates to another disorder-metabolic syndrome (MetS). However, little is known concerning the relation of visceral adiposity index (VAI)-a novel and simple indicator of VA, with OSA and MetS. The objective of the study was to analyze the association of VAI with both disorders and applicability to identify OSA patients at risk of MetS.Methods: Consecutive individuals undergoing polysomnography and biochemical tests were enrolled, and differences in all subjects grouped by apnea-hypopnea index (AHI) were analyzed. Spearman correlation was performed for assessing the relationship between VAI, OSA-related indices and metabolic score-total number of the positive diagnostic criteria of MetS. Receiver operating characteristic (ROC) curve was conducted to obtain a cut-off value of VAI for predicting incident MetS by sex. Then, the risk of MetS in OSA patients according to the cut-offs was attained by logistic regression.Results: A total of 411 individuals were enrolled. Of whom, 361 subjects were diagnosed OSA (mild in 67 patients, moderate in 89 and severe in 205, respectively). A significant increasing trend based on AHI was observed in the variables of blood pressure, triglycerides, fasting glucose, incident MetS, metabolic score and VAI (all p < 0.05). Irrespective of gender, VAI was all significantly correlated with PSG characteristics as AHI, mean nocturnal oxygen saturation, the lowest oxygen saturation, metabolic score(all p < 0.05). A VAI of 2.282, 2.105, 2.511 (for all subjects, males and females, separately) were calculated to determine the occurrence of MetS. According to the cut-offs, OSA patients tended to suffer from greater risk in MetS (odds ratio [OR] = 10.237, p = 0.000; OR = 13.556, p = 0.000; OR = 21.458, p = 0.000).Conclusions: The present study suggested that VAI was significantly associated with MetS and OSA. As a simple and alternative approach obtained in everyday practice, it may offer a powerful tool to identify patients with OSA at risk of MetS. [ABSTRACT FROM AUTHOR]- Published
- 2016
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39. Small intestinal submucosa-derived extracellular matrix bioscaffold significantly enhances angiogenic factor secretion from human mesenchymal stromal cells.
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Xin Lin, Robinson, Mikella, Petrie, Tye, Spandler, Veronica, Boyd, W. Douglas, and Sondergaard, Claus Svane
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MESENCHYMAL stem cells , *VASCULAR endothelial growth factors , *EXTRACELLULAR matrix , *SMALL intestine physiology , *MUCOUS membranes , *ENZYME-linked immunosorbent assay , *CYTOKINES - Abstract
Introduction: The in vivo therapeutic effect of mesenchymal stromal cells (MSCs) is currently believed to be tightly linked to their paracrine secretion ability. However, insufficient or imprecise cell delivery, low cell survival and retention post-transplant, along with harsh donor site microenvironments, are major barriers to the clinical success of MSC therapies. Here we tested a small intestinal submucosa (SIS)-derived extracellular matrix (ECM) bioscaffold augmented with MSCs, with the hypothesis that they will facilitate the precise delivery of increased numbers of MSCs therefore improving cell viability and retention. Methods: In this study, we evaluated the secretion of angiogenic factors from three human MSC lines cultured on SIS ECM. We used human antibody array and enzyme-linked immunosorbent assay to measure the level of angiogenic factors released from MSCs when cultured on SIS ECM or regular tissue culture plastic. We tested MSCs cultured for three different time points. Results: We found that the SIS ECM culture environment can significantly enhance the release of several angiogenic factors when compared to MSCs cultured on standard tissue culture plastic. Specifically, vascular endothelial growth factor and interleukin-8 secretion was significantly increased at 24, 48 and 72 hours postseeding onto SIS ECM whereas vascular endothelial growth factor release for cells cultured on plastic surface remained the same during these time points. We also observed significant donor to donor variation in cytokine production. Conclusions: This study demonstrates that MSCs transplanted onto a SIS ECM may greatly increase their therapeutic potential through an increase in pro-angiogenic cytokine release. [ABSTRACT FROM AUTHOR]
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- 2015
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40. CD146 as an adverse prognostic factor in uterine sarcoma.
- Author
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Yun Zhou, He Huang, Lin-Jing Yuan, Ying Xiong, Xin Huang, Jia-Xin Lin, and Min Zheng
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UTERINE cancer -- Patients ,UTERINE cancer ,IMMUNOHISTOCHEMISTRY ,LEIOMYOSARCOMA ,CANCER cells ,PROGNOSIS - Abstract
Background: Uterine sarcoma is an aggressive malignancy with a poor prognosis. This study aimed to determine the expression of CD146, P53, and Ki-67 in uterine sarcoma and to evaluate their prognostic significance. Methods: We retrospectively analyzed the prognosis and clinicopathologic features of 68 patients with uterine sarcoma. Immunohistochemical analyses of CD146, P53, and Ki-67 were performed in tissue samples collected from these patients and their relationship with prognosis was investigated. Results: The 5-year overall survival (OS) rate was 46 %. Endometrial stromal sarcoma (ESS) patients had a better prognosis than leiomyosarcoma (LMS) patients, with a 2-year survival rate of 82 %. The membrane and cytoplasm of tumor cells exhibited CD146 overexpression in 8 (32 %) ESS cases, which was less than the 25 (69.4 %) cases observed in LMS and 2 (28.6 %) in MMMT. CD146 overexpression in the membrane and cytoplasm of tumor cells was closely related to lymph node metastasis (P = 0.021) and Ki-67 overexpression (P = 0.0053); there was no significant correlation with age, tumor size, International Federation of Obstetrics and Gynecology stage, or P53 overexpression in LMS. Conclusions: CD146, P53, and Ki-67 are overexpressed in uterine sarcoma. CD146 expression correlates with lymph node metastasis and is associated with poor OS in LMS; it may be a potential prognostic marker for LMS. [ABSTRACT FROM AUTHOR]
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- 2015
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41. Safety and efficacy of anti-PCSK9 antibodies: a meta-analysis of 25 randomized, controlled trials.
- Author
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Xin-Lin Zhang, Qing-Qing Zhu, Li Zhu, Jian-Zhou Chen, Qin-Hua Chen, Guan-Nan Li, Jun Xie, Li-Na Kang, and Biao Xu
- Subjects
- *
THERAPEUTIC use of immunoglobulins , *TREATMENT effectiveness , *LOW density lipoproteins , *THERAPEUTIC complications , *PLACEBOS , *EZETIMIBE , *THERAPEUTICS - Abstract
Background: Inhibition of proprotein convertase subtilisin/kexin type 9 (PCSK9) has been intensively studied to lower low-density lipoprotein cholesterol (LDL-C) levels. The purpose of this meta-analysis was to evaluate the safety and efficacy of anti-PCSK9 antibodies in randomized, controlled trials (RCTs). Methods: PubMed, EMBASE, CENTRAL databases, and recent conferences were searched. Safety outcomes were rates of common adverse events. Efficacy outcomes included percentages of LDL-C lowering and other lipid changes compared with placebo and ezetimibe, respectively. Results: Twenty-five RCTs encompassing 12,200 patients were included. The rates of common adverse events were firstly reported in our study by pooling together all evidence in RCTs, showing largely no significant difference between anti-PCSK9 antibodies and placebo (or ezetimibe), except that alirocumab was associated with reduced rates of death (relative risk (RR): 0.43, 95 % confidence interval (CI): 0.19 to 0.96, P = 0.04) and an increased rate of injection-site reactions (RR: 1.48, 95 % CI: 1.05 to 2.09, P = 0.02); evolocumab reduced the rate of abnormal liver function (RR: 0.43, 95 % CI: 0.20 to 0.93, P = 0.03), both compared with placebo. No significant difference in safety outcomes was detected between monthly 420 mg and biweekly 140 mg evolocumab treatments. Monthly 420 mg evolocumab treatment significantly reduced LDL-C by -54.6 % (95 % CI: -58.7 to -50.5 %) and by absolute -78.9 mg/dl (95 % CI: -88.9 to -68.9 mg/dl) versus placebo, and by -36.3 % (95 % CI: -38.8 to -33.9 %) versus ezetimibe, and increased high-density lipoprotein cholesterol (HDL-C) by 7.6 % (95 % CI: 5.7 to 9.5 %) versus placebo and 6.4 % (95 % CI: 4.3 to 8.4 %) versus ezetimibe. An equal or even greater change was observed following biweekly 140 mg administration. Significant and favorable changes were also detected in other lipids following evolocumab treatment. Biweekly 50 to 150 mg alirocumab lowered LDL-C by -52.6 % (95 % CI: -58.2 to -47.0 %) versus placebo, by -29.9 % (95 % CI: -32.9 to -26.9 %) versus ezetimibe, and increased HDL-C by 8.0 % (95 % CI: 4.2 to 11.7 %) versus placebo. Conclusions: Evolocumab and alirocumab were safe and well-tolerated from our most-powered analyses. Both antibodies substantially reduced the LDL-C level by over 50 %, increased the HDL-C level, and resulted in favorable changes in other lipids. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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42. An integrative analysis of post-translational histone modifications in the marine diatom Phaeodactylum tricornutum.
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Veluchamy, Alaguraj, Rastogi, Achal, Xin Lin, Lombard, Bérangère, Murik, Omer, Thomas, Yann, Dingli, Florent, Rivarola, Maximo, Ott, Sandra, Xinyue Liu, Sun, Yezhou, Rabinowicz, Pablo D., McCarthy, James, Allen, Andrew E., Loew, Damarys, Bowler, Chris, and Tirichine, Leïla
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- 2015
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43. Number of negative lymph nodes is associated with disease-free survival in patients with breast cancer.
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San-Gang Wu, Jia-Yuan Sun, Juan Zhou, Feng-Yan Li, Qin Lin, Huan-Xin Lin, Xun-Xing Guan, and Zhen-Yu He
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BREAST cancer patients ,BREAST cancer prognosis ,LYMPH nodes ,PROGRESSION-free survival ,MASTECTOMY ,RETROSPECTIVE studies - Abstract
Background: The aim of this study was to evaluate the prognostic value of the number of negative lymph nodes (NLNs) in breast cancer patients after mastectomy. Methods: 2,455 breast cancer patients who received a mastectomy between January 1998 and December 2007 were retrospectively reviewed. The prognostic impact of the number of NLNs with respect to disease-free survival (DFS) was analyzed. Results: The median follow-up time was 62.0 months, and the 5-year and 10-year DFS was 87.1% and 74.3%, respectively. The DFS of patients with >10 NLNs was significantly higher than that of patents with ≤10 NLNs, and the 5-year DFS rates were 87.5% and 69.5%, respectively (P < 0.001). Univariate Cox analysis showed that the NLN count (continuous variable) was a prognostic factor of DFS (hazard ratio [HR] = 0.913, 95% confidence interval [CI]: 0.896-0.930, P < 0.001). In multivariate Cox analysis, patients with a higher number of NLNs had a better DFS (HR = 0.977, 95% CI: 0.958-0.997, P = 0.022). Subgroup analysis showed that the NLN count had a prognostic value in patients at different pT stages and pN positive patients (log-rank P < 0.001). However, it had no prognostic value in pN0 patients (log-rank P = 0.684). Conclusions: The number of NLNs is an independent prognostic factor of DFS in breast cancer patients after mastectomy, and patients with a higher number of NLNs have a better DFS. [ABSTRACT FROM AUTHOR]
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- 2015
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44. Dosimetric analysis of the brachial plexus among patients with breast cancer treated with postmastectomy radiotherapy to the ipsilateral supraclavicular area: report of 3 cases of radiationinduced brachial plexus neuropathy.
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San-Gang Wu, Si-Juan Huang, Juan Zhou, Jia-Yuan Sun, Han Guo, Feng-Yan Li, Qin Lin, Huan-Xin Lin, and Zhen-Yu He
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BRACHIAL plexus ,BREAST cancer patients ,RADIOTHERAPY ,MEDICAL dosimetry ,RADIATION carcinogenesis - Abstract
Background The purpose of this study was to evaluate the brachial plexus (BP) dose of postmastectomy radiotherapy (PMRT) to the ipsilateral supraclavicular (ISCL) area, and report the characteristics of radiation-induced brachial plexus neuropathy (RIBPN). Methods The BP dose of 31 patients who received adjuvant PMRT to the ISCL area and chest wall using three-dimensional conformal radiotherapy (3DCRT) and the records of 3 patients with RIBPN were retrospectively analyzed based on the standardized Radiation Therapy Oncology Group-endorsed guidelines. The total dose to the ISCL area and chest wall was 50 Gy in 25 fractions. Results Patients with a higher number of removed lymph nodes (RLNs) had a higher risk of RIBPN (hazard ratio [HR]: 1.189, 95% confidence interval [CI]: 1.005-1.406, p = 0.044). In 31 patients treated with 3DCRT, the mean dose to the BP without irradiation to the ISCL area was significantly less than that with irradiation to the ISCL area (0.97 ± 0.20 vs. 44.39 ± 4.13 Gy, t = 136.75, p <0.001). In the 3DCRT plans with irradiation to the ISCL area and chest wall, the maximum dose to the BP was negatively correlated with age (r = −0.40, p = 0.026), body mass index (BMI) (r = −0.44, p = 0.014), and body weight (r = −0.45, p = 0.011). Symptoms of the 3 patients with RIBPN occurred 37–65 months after radiotherapy, and included progressive upper extremity numbness, pain, and motor disturbance. After treatment, 1 patient was stable, and the other 2 patients' symptoms worsened. Conclusions The incidence of RIBPN was higher in patients with a higher number of RLNs after PMRT. The dose to the BP is primarily from irradiation of the ISCL area, and is higher in slim and young patients. Prevention should be the main focus of managing RIBPN, and the BP should be considered an organ-at-risk when designing a radiotherapy plan for the ISCL area. [ABSTRACT FROM AUTHOR]
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- 2014
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45. Number of negative lymph nodes can predict survival of breast cancer patients with four or more positive lymph nodes after postmastectomy radiotherapy.
- Author
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San-Gang Wu, Jia-Yuan Sun, Juan Zhou, Feng-Yan Li, Hao Zhou, Qin Lin, Huan-Xin Lin, Yong Bao, and Zhen-Yu He
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LYMPH nodes ,BREAST cancer prognosis ,BREAST cancer patients ,MASTECTOMY ,RADIOTHERAPY ,PROGRESSION-free survival ,REGRESSION analysis - Abstract
Background This study was conducted to assess the prognostic value of the number of negative lymph nodes (NLNs) in breast cancer patients with four or more positive lymph nodes after postmastectomy radiotherapy (PMRT). Methods This retrospective study examined 605 breast cancer patients with four or more positive lymph nodes who underwent mastectomy. A total of 371 patients underwent PMRT. The prognostic value of the NLN count in patients with and without PMRT was analyzed. The log-rank test was used to compare survival curves, and Cox regression analysis was performed to identify prognostic factors. Results The median follow-up was 54 months, and the overall 8-year locoregional recurrence-free survival (LRFS), distant metastasis-free survival (DMFS), disease-free survival (DFS), and overall survival (OS) were 79.8%, 50.0%, 46.8%, and 57.9%, respectively. The optimal cutoff points for NLN count was 12. Univariate analysis showed that the number of NLNs, lymph node ratio (LNR) and pN stage predicted the LRFS of non-PMRT patients (p < 0.05 for all). Multivariate analysis showed that the number of NLNs was an independent prognostic factor affecting the LRFS, patients with a higher number of NLNs had a better LRFS (hazard ratio = 0.132, 95% confidence interval = 0.032-0.547, p =0.005). LNR and pN stage had no effect on LRFS. PMRT improved the LRFS (p < 0.001), DMFS (p = 0.018), DFS (p = 0.001), and OS (p = 0.008) of patients with 12 or fewer NLNs, but it did not any effect on survival of patients with more than 12 NLNs. PMRT improved the regional lymph node recurrence-free survival (p < 0.001) but not the chest wall recurrence-free survival (p = 0.221) in patients with 12 or fewer NLNs. Conclusions The number of NLNs can predict the survival of breast cancer patients with four or more positive lymph nodes after PMRT. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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46. Translation and validation of the Chinese version of the quality of life radiation therapy instrument and the head & neck module (QOL-RTI/H&N).
- Author
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Xin-lin Chen, Zhen-wen Qiu, Mo-fa Gu, Yong Su, Li-zhi Liu, Yan Liu, Chuan-wei Mo, Qian Xu, Juan Sun, and Dong-hai Li
- Subjects
- *
CANCER radiotherapy , *HEAD & neck cancer treatment , *QUALITY of life , *CHINESE people , *MEDICAL equipment , *PSYCHOMETRICS , *DISEASES - Abstract
Background: To translate and validate the Chinese version of the Quality Of Life Radiation Therapy Instrument and the Head & Neck Module (QOL-RTI/H&N), a disease-specific scale to measure quality of life (QOL) for patients with head and neck cancer (HNC) who received radiotherapy. Methods: The QOL-RTI/H&N was translated and validated according to the standard process: a translation and back-translation procedure, pilot testing and a validation study. HNC patients were enrolled from the Cancer Center of Sun Yat-sen University and assessed using the QOL-RTI/H&N, QLQ-C30 and QLQ-H&N35. Reliability (internal consistency reliability, split-half reliability and test-retest reliability), validity (content validity, construct validity, criterion validity and discriminant validity), and responsiveness analysis were performed to evaluate the psychometric characteristics of the QOL-RTI/H&N. Results: A total of 238 patients (99.2%) completed the questionnaire. Item RTI23 had 16.0% missing data. Other items had low percentages of missing data (0.4% or 0.8%) or no missing data. The average time to finish the scale was 9.8 minutes. Cronbach's alpha of the domains ranged from 0.41 to 0.77. The split-half reliability coefficients ranged from 0.43 to 0.77. All of the intra-class correlation coefficients were equal to or greater than 0.8. All of the item-own domain correlation coefficients were greater than those of the item-other domain. Confirmatory factor analysis showed that Comparative Fit Index, Normed Fit Index and Non- Normed Fit Index were equal to 1.00. Root Mean Square Error of Approximation was 0.01, with 90% CI (0.00, 0.10). The domain scores of the QOL-RTI/H&N were significantly correlated with those of the QLQ-C30 or QLQ-H&N3. All domain scores of patients in different radiotherapy stages were statistically significant (P < 0.05), apart from the speech domain. Conclusions: The Chinese version of the QOL-RTI/H&N is a valid, reliable and responsive scale to measure QOL in HNC patients and can be used to assess the effects of radiotherapy treatment on these patients. [ABSTRACT FROM AUTHOR]
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- 2014
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47. The role of quality of care and attitude towards disability in the relationship between severity of disability and quality of life: findings from a crosssectional survey among people with physical disability in China.
- Author
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Qiao-Lan Zheng, Qi Tian, Chun Hao, Jing Gu, Lucas-Carrasco, Ramona, Jian-Ting Tao, Zuo-Yi Liang, Xin-Lin Chen, Ji-Qian Fang, Jian-Hua Ruan, Qiu-Xiang Ai, and Yuan-Tao Hao
- Subjects
PEOPLE with disabilities ,QUALITY of life ,CROSS-sectional method ,STRUCTURAL equation modeling ,MEDICAL quality control - Abstract
Background People with physical disability (PWPD) is the largest subgroup of people with disability (PWD) in China, but few studies have been conducted among this vulnerable population. The objective of this study was to investigate the level of quality of life (QoL), self-perceived quality of care and support (QOCS), severity of disability and personal attitude towards disability among people with physical disability in China, as well as to identify how QoL can be affected by severity of disability through QOCS and personal attitude towards disability among PWPD. Methods A cross-sectional study was conducted among 1,853 PWPD in Guangzhou, China. Data were collected on participants' QoL, QOCS, personal attitude towards disability and severity of disability. Structural equation modeling was used to examine the effects of the other variables on QoL. Results Even with a mild disability (mean score:1.72), relatively low levels of QoL (mean score: 2.65- 3.22) and QOCS (mean score: 2.95 to 3.28), as well as unfavorable personal attitude towards disability (mean score: 2.75 to 3.36) were identified among PWPD. According to SEM, we found that the influence of severity of physical disability on QoL is not only exerted directly, but is also indirectly through QOCS and their personal attitudes towards disability, with QOCS playing a more important mediating role than PWPD's attitudes towards their own disability. Conclusions Unfavorable health status was identified among PWPD in China. Focusing on improvement of assistance and care services has the potential to substantially improve PWPD's QoL. Further research should focus on understanding the needs and their current state of health care of PWPD in China thus being able to develop better interventions for them. [ABSTRACT FROM AUTHOR]
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- 2014
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48. Analysis of the impact of platinum-based combination chemotherapy in small cell cervical carcinoma: a multicenter retrospective study in Chinese patients.
- Author
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Long Huang, Ling-Min Liao, An-Wen Liu, Jian-Bing Wu, Xiao-Ling Cheng, Jia-Xin Lin, and Min Zheng
- Subjects
PLATINUM ,CANCER chemotherapy ,CERVICAL cancer treatment ,CHINESE people ,HOSPITAL admission & discharge ,RETROSPECTIVE studies ,COMPARATIVE studies ,DISEASES - Abstract
Background Small cell cervical carcinoma (SCCC) is a rare, aggressive tumor with a poor prognosis. However, information in relation to its treatment is scarce due to the limited numbers of patients. The aim of this study was to establish whether platinum-based combination chemotherapy may by beneficial in this patient population. Methods We carried out a multicenter, retrospective study comprising of 72 Chinese patients with SCCC. The patients were treated between 1995 and 2010 at Sun Yat-sen Memorial Hospital or the Cancer Center of Sun Yat-Sen University, and at the First Affiliated Hospital of Shantou University Medical College, China. Results Of the 72 patients, 46/72 (63.9%) had Federation of Gynecology and Obstetrics (FIGO) stage Ia-Ib2 and 26/72 (36.1%) had stage IIa-IV disease. Surgery was performed in 63/72 (87.5%) patients, 61/72 (84.7%) patients received chemoradiotherapy and 35/72 (48.6%) received radiotherapy. The 3-year overall survival (OS) and disease-free survival (DFS) rates were as follows: Ia (100%, 100%); Ib1 (62%, 57%); Ib2 (53%, 48%); IIa (36%, 23%); IIb (29%, 21%); IIIb (50%, 50%); and IV (0%, 0%), respectively. The estimated 3-year OS and DFS rates in patients who received platinum-based combination chemotherapy (etoposide + cisplatin [EP], or paclitaxel + cisplatin [TP]) as part of their adjuvant treatment were 64.8% and 63.0%, respectively, compared to 25.2% and 22.0% in those who did not (P = 0.0003; P = 0.0003). Univariate analysis showed that platinum-based combination chemotherapy was associated with improved survival compared to other chemotherapy techniques or no chemotherapy (OS: HR = 0.227; 95% CI, 0.099-0.524; P = 0.001; DFS: HR = 0.210; 95% CI, 0.087-0.506; P = 0.001). Multivariate analysis identified FIGO stage, lymphatic metastasis and platinum-based combination chemotherapy as independent prognostic factors for improved survival in patients with SCCC. Conclusions Platinum-based combination chemotherapy (with EP or TP) can improve the 3-year survival outcomes in patients with SCCC. Therefore, it should be considered an important component in a future standardized treatment strategy for SCCC. [ABSTRACT FROM AUTHOR]
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- 2014
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49. Inflammatory myofibroblastic tumor with extensive involvement of the bladder in an adolescent: a case report.
- Author
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Xin Lin Yi, Hao Yuan Lu, Yue Xian Wu, Wen Hui Li, Qing Gui Meng, Ji Weng Cheng, Yong Tang, Yu Liu, and Xian Zhong Bai
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MYOFIBROBLASTS , *PERITONEAL cancer , *RETROPERITONEUM diseases , *COMPUTED tomography - Abstract
Inflammatory myofibroblastic tumor (IMT) is a rare lesion of unclear pathogenesis that shows a wide, highly variable spectrum of clinical behavior. We describe the case of a 17-year-old boy with a large IMT that infiltrated the bladder, ileocecal junction, peritoneum and pelvic retroperitoneal space. The tumor was associated with extensive toughening and thickening of the bladder, and, although it showed a tendency for invasive growth, it affected mainly the bladder and adjacent tissue. To the best of our knowledge, this case report is the first to describe an IMT involving the entire bladder and several adjacent pelviabdominal organs. The bladder wall was tough and could hardly be cut by scalpel. Levels of inflammatory response markers such as C-reactive protein fell after surgery. [ABSTRACT FROM AUTHOR]
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- 2013
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50. Flotillin-2 is associated with breast cancer progression and poor survival outcomes.
- Author
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Xi Wang, Qi Yang, Ling Guo, Xing-Hua Li, Xiao-Hui Zhao, Li-Bing Song, and Huan-Xin Lin
- Subjects
BREAST cancer treatment ,DISEASE progression ,CANCER cells ,EPITHELIAL cells ,CELL lines ,WESTERN immunoblotting ,POLYMERASE chain reaction ,IMMUNOHISTOCHEMISTRY - Abstract
Background: Flotillin-2 (FLOT2) has been implicated in several signaling pathways in tumor cells. Our study aimed to investigate the expression pattern and clinicopathological significance of FLOT2 in patients with breast cancer. Methods: The expression level of FLOT2 in normal breast epithelial cells, breast cancer cell lines, and four breast cancer biopsies paired with adjacent noncancerous tissues were quantified using real-time RT-PCR and Western blotting. FLOT2 protein expression was analyzed in 171 archived paraffin-embedded breast cancer samples using immunohistochemistry (IHC). Statistical analyses were performed to evaluate the clinicopathological significance of FLOT2 expression. Results: FLOT2 was significantly upregulated in breast cancer cell lines and tissue samples compared with normal cells and adjacent noncancerous breast tissues, respectively. IHC analysis revealed high expression levels of FLOT2 in 82 of 171 (48.0%) breast cancer specimens. Statistical analysis revealed that FLOT2 expression was significantly correlated with clinical stage (P < 0.001), T classification (P < 0.001), M classification (P < 0.001), histological differentiation (P = 0.005) and ErbB2 expression (P = 0.003). Patients with higher levels of FLOT2 expression had a shorter overall survival duration than patients with lower FLOT2 expression levels. Multivariate analysis suggested that FLOT2 expression was an independent prognostic marker for survival in patients with breast cancer. Conclusions: The current results demonstrated that high FLOT2 protein expression was associated with poor outcomes in patients with breast cancer. FLOT2 could be used as a prognostic biomarker for breast cancer progression. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
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