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Your search keyword '"Xiao-jie Chen"' showing total 2 results
Start Over Author "Xiao-jie Chen" Publisher biomed central
2 results on '"Xiao-jie Chen"'

1. Absence of high-risk HPV 16 and 18 in Chinese patients with oral squamous cell carcinoma and oral potentially malignant disorders

Author

Xiao-Jie Chen, Wei-Wen Jiang, and Kai Sun

Subjects
0301 basic medicine, Oncology, Male, viruses, Bioinformatics, 0302 clinical medicine, Prevalence, Medicine, Aged, 80 and over, Human papillomavirus 16, Human papillomavirus 18, HPV infection, virus diseases, Middle Aged, female genital diseases and pregnancy complications, DNA-Binding Proteins, Infectious Diseases, Oral squamous cell carcinoma, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, Female, Mouth Neoplasms, HPV16, Adult, medicine.medical_specialty, China, HPV18, Oral potentially malignant disorders, Real-Time Polymerase Chain Reaction, DNA sequencing, 03 medical and health sciences, Asian People, Internal medicine, Virology, Humans, Basal cell, Human papillomavirus, Aged, business.industry, Research, Papillomavirus Infections, Cancer, Oncogene Proteins, Viral, Sequence Analysis, DNA, medicine.disease, Repressor Proteins, stomatognathic diseases, 030104 developmental biology, High risk hpv, business
Abstract

Background The critical role of human papillomavirus (HPV) in cancer has been recognized, but the involvement of HPV in oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMD) is still controversial. The aim of this study was to identify and verify the prevalence of high-risk HPV infection (HPV16 and 18) in Chinese patients with OSCC or OPMD using real-time PCR and DNA sequencing. Methods Paired tissue and serum DNA samples were extracted from 40 Chinese patients with OSCC and 59 with OPMD. A SYBR Green-based real-time PCR assay was developed to detect the E6 gene of HPV16 and HPV18. Suspicious positive samples were then sequenced to eliminate false positives. Results We found that none of the tissue and serum samples of OSCCs and OPMDs were positive for HPV16 E6 or 18 E6, using both real-time PCR and DNA sequencing. Overall, 3 of 198 (1.52 %) and 7 of 198 (3.54 %) samples were false-positive for HPV16 E6 and HPV18 E6, respectively, using real-time PCR. Conclusion The lack of HPV16 and HPV18 detected in this study indicates that high-risk HPV 16 and 18 infections are uncommon in Chinese patients with OSCC and OPMD. Real-time PCR followed by DNA sequencing for HPV DNA detection is an effective strategy to rule out false positives.

Published
2016

2. Pulmonary embolism in non-brain tumor patients after surgery--a retrospective study in China.

Author

Ren-Xiong Chen, Hong-Zhi Wang, Jun Dong, Hong Ren, Xiao-Jie Chen, Jia-Xuan Xu, Yong Yang, and Guo-Dong Wang

Subjects
PULMONARY embolism, BRAIN tumor treatment, SURGICAL technology, RETROSPECTIVE studies, PROPORTIONAL hazards models, DIAGNOSIS
Abstract

Background: The incidence rate of pulmonary emboli (PE) is high in tumor patients; however, the morbidity and mortality associated with the development of PE after tumor surgery are unknown. We studied the clinical profiles and outcomes of patients with PE after non-brain tumor surgery. Methods: We retrospectively screened 55,967 patients who underwent non-brain tumor surgery at the Peking University Cancer Hospital from January 2008 to June 2015. Among them, 76 patients who were diagnosed with PE were enrolled in our study. Factors impacting the overall survival at 90 days were analyzed. A Kaplan-Meier curve was plotted for time to death or until day 90. Cox proportional hazard modeling was performed for univariate-and multivariate-adjusted factor analyses. Results: The morbidity rate was approximately 135.8 per 100,000 non-brain tumor surgery patients (possibly underestimated). When treated, seven patients had major bleeding, and 14 patients had clinically relevant non-major bleeding, which represented 9.2 and 18.4% of all the patients, respectively. The 3-month overall mortality rate was 11.8% in our study. The Acute Physiology and Chronic Health Evaluation II (APACHE II) score and platelet distribution width (PDW) were independent risk factors for the prognosis of PE after nonbrain surgery (P values of 0.001 and 0.016, respectively). Conclusions: Treatment of PE in non-brain tumor surgical patients remained a challenge due to the high bleeding rate. The APACHE II score and PDW were independent prognostic factors of survival in patients with PE after non-brain tumor surgery; however, the study power was limited. [ABSTRACT FROM AUTHOR]

Published
2017
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