183 results on '"Xiao Jiang"'
Search Results
2. Pathological insights from amyotrophic lateral sclerosis animal models: comparisons, limitations, and challenges
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Zhu, Longhong, Li, Shihua, Li, Xiao-Jiang, and Yin, Peng
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- 2023
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3. The association of vitamin D deficiency, age and depression in US adults: a cross-sectional analysis
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Mo, Hongfei, Zhang, Jipeng, Huo, Chiwei, Zhang, Mengying, Xiao, Jiang, Peng, Junge, Wang, Guirong, Wang, Changhong, and Li, Yan
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- 2023
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4. Prevalence and risk factors for kidney disease among hospitalized PLWH in China
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Zhao, Naxin, Xiang, Pan, Zeng, Zhili, Liang, Hongyuan, Wang, Fang, Xiao, Jiang, Yang, Di, Wang, Sa, Chen, Meiling, and Gao, Guiju
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- 2023
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5. Stroke among highly active antiretroviral therapy-naive people living with the human immunodeficiency virus in China: a retrospective study of the characteristics, risk factors, and prognosis
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Zhang, Ling, Wang, Yu, Xu, Qiuhua, Zhang, Wei, Liang, Hongyuan, Wu, Liang, Ni, Liang, Gao, Guiju, Yang, Di, Zhao, Hongxin, and Xiao, Jiang
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- 2022
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6. Correction: Estrogen signaling effects on muscle-specific immune responses through controlling the recruitment and function of macrophages and T cells
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Liao, Zhao Hong, Huang, Tao, Xiao, Jiang Wei, Gu, Rui Cai, Ouyang, Jun, Wu, Gang, and Liao, Hua
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- 2022
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7. Cytomegalovirus cell tropism and clinicopathological characteristics in gastrointestinal tract of patients with HIV/AIDS
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Sun, Lei, Chen, Jia-min, Yang, Kun, Zhang, Liang, Ma, Zhi-yuan, Chen, Xiang-mei, Li, Man, Zhou, Xingang, Li, Ping, Zhao, Hong-xin, Xiao, Jiang, Qi, Li-ming, and Wang, Peng
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- 2022
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8. Genetically modified large animal models for investigating neurodegenerative diseases
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Yang, Weili, Chen, Xiusheng, Li, Shihua, and Li, Xiao-Jiang
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- 2021
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9. Risk factors of in-stent restenosis among coronary artery disease patients with syphilis undergoing percutaneous coronary intervention: a retrospective study
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Zhang, Ling, Wang, Yu, Zhang, Zhe, Liang, Hongyuan, Wu, Liang, Ni, Liang, Gao, Guiju, Yang, Di, Zhao, Hongxin, and Xiao, Jiang
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- 2021
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10. Comparison of the prognosis of four different treatment strategies for acute left malignant colonic obstruction: a systematic review and network meta-analysis
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Tan, Ling, Liu, Zi-lin, Ran, Meng-ni, Tang, Ling-han, Pu, Yan-jun, Liu, Yi-lei, Ma, Zhou, He, Zhou, and Xiao, Jiang-wei
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- 2021
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11. Palliative Gastrectomy versus Gastrojejunostomy for advanced Gastric cancer with outlet obstruction: a propensity score matching analysis
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Chen, Xiao-Jiang, Chen, Guo-Ming, Wei, Yi-Cheng, Yu, Hong, Wang, Xi-Cheng, Zhao, Zhou-Kai, Luo, Tian-Qi, Nie, Run-Cong, and Zhou, Zhi-Wei
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- 2021
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12. The clinical characteristics and outcome of cryptococcal meningitis with AIDS in a tertiary hospital in China: an observational cohort study
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Wu, Liang, Xiao, Jiang, Song, Yangzi, Gao, Guiju, and Zhao, Hongxin
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- 2020
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13. Programmed death-1 expression and regulatory T cells increase in the Intestinal mucosa of cytomegalovirus colitis in patients with HIV/AIDS
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Sun, Lei, Yang, Kun, Zhang, Liang, Qi, Li-ming, Chen, Jia-min, Li, Ping, Xiao, Jiang, Zhao, Hong-xin, and Wang, Peng
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- 2020
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14. Independent risk factors for axillary lymph node metastasis in breast cancer patients with one or two positive sentinel lymph nodes
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Zhang, Wei, Xu, Jing, Wang, Ke, Tang, Xiao-Jiang, Liang, Hua, and He, Jian-Jun
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- 2020
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15. Disease-free survival as a surrogate endpoint for overall survival in adjuvant trials of pancreatic cancer: a meta-analysis of 20 randomized controlled trials
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Nie, Run-Cong, Zou, Xue-Bin, Yuan, Shu-Qiang, Chen, Ying-Bo, Chen, Shi, Chen, Yong-Ming, Chen, Guo-Ming, Chen, Xiao-Jiang, Luo, Tian-Qi, Li, Shu-Man, Duan, Jin-Ling, Wang, Yun, and Li, Yuan-Fang
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- 2020
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16. Analysis of the causes of cervical lymphadenopathy using fine-needle aspiration cytology combining cell block in Chinese patients with and without HIV infection
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Sun, Lei, Zhang, Liang, Yang, Kun, Chen, Xiang-mei, Chen, Jia-min, Xiao, Jiang, Zhao, Hong-xin, Ma, Zhi-yuan, Qi, Li-ming, and Wang, Peng
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- 2020
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17. Estrogen signaling effects on muscle-specific immune responses through controlling the recruitment and function of macrophages and T cells
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Liao, Zhao Hong, Huang, Tao, Xiao, Jiang Wei, Gu, Rui Cai, Ouyang, Jun, Wu, Gang, and Liao, Hua
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- 2019
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18. Mitochondrial disturbance related to increased caspase-1 of CD4+T cells in HIV-1 infection.
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Yu, Fengting, Ma, Chengjie, Jin, Xia, Zhao, Hongxin, Xiao, Jiang, Li, Li, Song, Shujing, Xie, Xiaohui, Yang, Siyuan, Tang, Yunxia, Wang, Linghang, and Zhang, Fujie
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CASPASES ,T cell differentiation ,HIV ,MITOCHONDRIA ,GENE expression - Abstract
Background: In HIV-1 infection, more than 95% of CD4
+ T cells die of caspase-1 mediated pyroptosis. What governs the increased susceptibility of CD4+ T cells to pyroptosis is poorly understood. Methods: Blood samples were obtained from 31 untreated HIV-infected patients (UNT), 29 antiretroviral therapy treated HIV-infected patients (ART), and 21 healthy control donors (HD). Plasma levels of IL-18 and IL-1β, caspase-1 expression, mitochondrial mass (MM) and mitochondrial fusion/fisson genes of CD4+ T subsets were measured. Results: A significantly higher IL-18 level in plasma and MM level of CD4+ T cells were found in HIV-infected patients (UNT and ART) compared to HD, and the MMhigh phenotype was manifested, related to increased caspase-1 expression. Moreover, the increased MM was more pronounced in the early differentiated and inactivated CD4+ T cells. However, higher MM was not intrinsically linked to T cell differentiation disorder or excessive activation of the CD4+ T cells. Mechanistically, the increased MM was significantly correlated with an elevated level of expression of the mitochondrial fusion gene mitofusin1. Conclusion: An increase in MM was associated with heightened sensitivity of CD4+ T cells to pyroptosis, even in early differentiated and inactivated CD4+ T cells, in patients with HIV-1 infection, regardless of whether patients were on antiretroviral therapy or not. These new revelations have uncovered a previously unappreciated challenge to immune reconstitution with antiretroviral therapy. [ABSTRACT FROM AUTHOR]- Published
- 2024
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19. Fast-track multidisciplinary treatment versus conventional treatment for colorectal cancer: a multicenter, open-label randomized controlled study
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Li, Jun, Kong, Xiang-Xing, Zhou, Jiao-Jiao, Song, Yong-Mao, Huang, Xue-Feng, Li, Gen-Hai, Ying, Xiao-Jiang, Dai, Xiao-Yu, Lu, Min, Jiang, Kai, Fu, Dong-Liang, Li, Xin-Lin, He, Jin-Jie, Wang, Jian-Wei, Sun, Li-Feng, Xu, Dong, Xu, Jing-Yan, Chen, Min, Tian, Yu, Li, Jing-Song, Yan, Min, Yuan, Ying, and Ding, Ke-Feng
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- 2019
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20. Selective ERK1/2 agonists isolated from Melia azedarach with potent anti-leukemic activity
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Wang, Ning, Fan, Yanhua, Yuan, Chun-Mao, Song, Jialei, Yao, Yao, Liu, Wuling, Gajendran, Babu, Zacksenhaus, Eldad, Li, Yanmei, Liu, Jielin, Hao, Xiao Jiang, and Ben-David, Yaacov
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- 2019
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21. A CRISPR monkey model unravels a unique function of PINK1 in primate brains
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Yang, Weili, Li, Shihua, and Li, Xiao-Jiang
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- 2019
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22. Simultaneous VENTANA IHC and RT-PCR testing of ALK status in Chinese non-small cell lung cancer patients and response to crizotinib
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Xu, Chun-wei, Wang, Wen-xian, Chen, Yan-ping, Chen, Yu, Liu, Wei, Zhong, Li-hua, Chen, Fang-fang, Zhuang, Wu, Song, Zheng-bo, Chen, Xiao-hui, Huang, Yun-jian, Guan, Yan-fang, Yi, Xin, Lv, Tang-feng, Zhu, Wei-feng, Lu, Jian-ping, Wang, Xiao-jiang, Shi, Yi, Lin, Xian-dong, Chen, Gang, and Song, Yong
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- 2018
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23. Bursectomy for advanced gastric cancer: an update meta-analysis
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Nie, Run-Cong, Yuan, Shu-Qiang, Chen, Shi, Yan, Shu-Mei, Chen, Yong-Ming, Chen, Xiao-Jiang, Chen, Guo-Ming, Zhou, Zhi-Wei, Chen, Ying-Bo, and Li, Yuan-Fang
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- 2018
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24. Selective ERK1/2 agonists isolated from Melia azedarach with potent anti-leukemic activity
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Yao Yao, Jielin Liu, Yaacov Ben-David, Chun-Mao Yuan, Babu Gajendran, Ning Wang, Jialei Song, Yanhua Fan, Xiao-Jiang Hao, Yanmei Li, Wuling Liu, and Eldad Zacksenhaus
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0301 basic medicine ,MAPK/ERK pathway ,Male ,Cancer Research ,Apoptosis ,Mice ,0302 clinical medicine ,Chinese medicinal plant ,Drug screen ,Receptor ,Cancer ,Mice, Inbred BALB C ,Leukemia ,Kinase ,Chemistry ,Cell Differentiation ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Cell biology ,Blot ,Molecular Docking Simulation ,Survival Rate ,Oncology ,030220 oncology & carcinogenesis ,Differentiation ,Disease Progression ,Phosphorylation ,Female ,Research Article ,Signal Transduction ,Limonins ,MAP Kinase Signaling System ,Melia azedarach ,lcsh:RC254-282 ,ERK1/2 agonists ,03 medical and health sciences ,Genetics ,Animals ,Humans ,Mitogen-Activated Protein Kinase Kinases ,Binding Sites ,Activator (genetics) ,Cell growth ,Cell Cycle Checkpoints ,Antineoplastic Agents, Phytogenic ,Plant Leaves ,Disease Models, Animal ,030104 developmental biology ,Cell culture ,Leukemia, Erythroblastic, Acute ,Drug Screening Assays, Antitumor ,K562 Cells ,Drugs, Chinese Herbal - Abstract
Background MAPK/ERK kinases transmit signals from many growth factors/kinase receptors during normal cell growth/differentiation, and their dysregulation is a hallmark of diverse types of cancers. A plethora of drugs were developed to block this kinase pathway for clinical application. With the exception of a recently identified agent, EQW, most of these inhibitors target upstream factors but not ERK1/2; no activator of ERK1/2 is currently available. Method A library of compounds isolated from medicinal plants of China was screened for anti-cancer activities. Three limonoid compounds, termed A1541–43, originally isolated from the plant Melia azedarach, exhibiting strong anti-leukemic activity. The anti-neoplastic activity and the biological target of these compounds were explored using various methods, including western blotting, flow cytometry, molecular docking and animal model for leukemia. Results Compounds A1541–43, exhibiting potent anti-leukemic activity, was shown to induce ERK1/2 phosphorylation. In contrast, the natural product Cedrelone, which shares structural similarities with A1541–43, functions as a potent inhibitor of ERK1/2. We provided evidence that A1541–43 and Cedrelone specifically target ERK1/2, but not the upstream MAPK/ERK pathway. Computational docking analysis predicts that compounds A1541–43 bind a region in ERK1/2 that is distinct from that to which Cedrelone and EQW bind. Interestingly, both A1541–43, which act as ERK1/2 agonists, and Cedrelone, which inhibit these kinases, exerted strong anti-proliferative activity against multiple leukemic cell lines, and induced robust apoptosis as well as erythroid and megakaryocytic differentiation in erythroleukemic cell lines. These compounds also suppressed tumor progression in a mouse model of erythroleukemia. Conclusions This study identifies for the first time activators of ERK1/2 with therapeutic potential for the treatment of cancers driven by dysregulation of the MAPK/ERK pathway and possibly for other disorders. Electronic supplementary material The online version of this article (10.1186/s12885-019-5914-8) contains supplementary material, which is available to authorized users.
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- 2019
25. Tac2-N acts as a novel oncogene and promotes tumor metastasis via activation of NF-κB signaling in lung cancer
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Wen-bin Liu, Lin Ao, Fei Han, Ning Zhang, Li-Yun Gao, Jinyi Liu, Hongqiang Chen, Xiao Jiang, Xiang-Lin Hao, and Jia Cao
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0301 basic medicine ,Male ,Cancer Research ,Lung Neoplasms ,MMP9 ,Transfection ,lcsh:RC254-282 ,MMP7 ,Metastasis ,03 medical and health sciences ,Mice ,0302 clinical medicine ,In vivo ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Neoplasm Metastasis ,Lung cancer ,NF-κB signaling pathway ,Tissue microarray ,Oncogene ,business.industry ,Research ,NF-kappa B ,Nuclear Proteins ,Oncogenes ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Disease Models, Animal ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Tac2-N ,Cancer research ,Heterografts ,Ectopic expression ,Female ,business ,Signal Transduction - Abstract
Background High rates of recurrence and metastasis are the major cause of the poor outcomes for patients with lung cancer. In previous research, we have demonstrated that Tac2-N promotes tumor growth by suppressing p53 signaling in lung cancer. Beyond that, other biological functions and clinical significance of Tac2-N in lung cancer progression are still unknown. Methods Tissue microarrays of 272 lung cancer patients were constructed to assess the association of Tac2-N expression and prognosis of lung cancer patients with different clinical stages. The protein expression of Tac2-N in metastatic and non-metastatic specimens were detected by IHC. In vitro migration and invasion and in vivo nude mice metastasis model were used to evaluate the effect of Tac2-N ectopic expression on metastasis capability of lung cancer cells. The downstream signaling pathway of Tac2-N was explored using luciferase reporter assays and WB. Results The expression of Tac2-N was associated with advanced stages, but not with early stages (P = 0.513). Tac2-N expression is sharply overexpressed in metastatic tumors compared with non-metastatic tumors. In vitro and in vivo assays suggested that Tac2-N facilitated migration and invasion of lung cancer cells in vitro and promoted tumor metastasis in vivo. Mechanistically, Tac2-N increased the degradation of IκB by promoting its phosphorylation, and subsequently activated NF-κB activity by facilitating the nuclear translocation of NF-κB and stimulating the transcription of targets, MMP7 and MMP9. Notably, the C2B domain of Tac2-N was crucial for Tac2-N to activate NF-κB signal. Blockage of NF-κB by shRNA or inhibitor attenuates the function of Tac2-N in the promotion of metastasis. Conclusions Our study provided proof of principle to show that Tac2-N serves as a novel oncogene gene and plays an important role in the progression and metastasis of lung cancer. Electronic supplementary material The online version of this article (10.1186/s13046-019-1316-7) contains supplementary material, which is available to authorized users.
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- 2019
26. IVF outcomes of women with discrepancies between age and serum anti-Müllerian hormone levels
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Xiao Jiang, Huihui Zhang, Chao Liu, Linlin Cui, Zi-Jiang Chen, Yueru Meng, and Bingqian Zhang
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Anti-Mullerian Hormone ,endocrine system diseases ,Pregnancy Rate ,medicine.medical_treatment ,Oocyte Retrieval ,Miscarriage ,Cohort Studies ,Endocrinology ,Pregnancy ,AMH ,Medicine ,lcsh:Reproduction ,Live birth ,biology ,Obstetrics ,Age Factors ,Pregnancy Outcome ,Obstetrics and Gynecology ,Anti-Müllerian hormone ,Middle Aged ,Embryo transfer ,female genital diseases and pregnancy complications ,IVF ,Female ,hormones, hormone substitutes, and hormone antagonists ,Cohort study ,Maternal Age ,Adult ,medicine.medical_specialty ,endocrine system ,lcsh:QH471-489 ,Reproductive medicine ,Fertilization in Vitro ,lcsh:Gynecology and obstetrics ,Humans ,Ovarian reserve ,lcsh:RG1-991 ,In vitro fertilisation ,business.industry ,urogenital system ,Research ,Advanced age ,medicine.disease ,Embryo Transfer ,Reproductive Medicine ,biology.protein ,business ,Developmental Biology - Abstract
Background To determine the effects of age and the serum anti-Müllerian hormone (AMH) level on in vitro fertilization (IVF) outcomes, especially among young women with low serum AMH levels and older women with high AMH levels. Methods This study was a cohort study in which a total of 9431 women aged 20–51 years who were undergoing their first IVF cycles were recruited. Ovarian response parameters included the number of retrieved oocytes, the number of 2 pronuclear zygotes (2PN), and the frequency of good-quality embryos (GQE). Pregnancy outcomes included the clinical pregnancy rate (CPR), live birth rate (LBR), miscarriage rate (MR), and cumulative CPR and LBR (CCPR and CLBR). Results Among women under 35 years of age, the ovarian response, CPR, CCPR, LBR and CLBR (p
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- 2019
27. Bursectomy for advanced gastric cancer: an update meta-analysis
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Yuan Fang Li, Guo Ming Chen, Zhiwei Zhou, Run Cong Nie, Shu Mei Yan, Xiao Jiang Chen, Yingbo Chen, Shu Qiang Yuan, Shi Chen, and Yong Ming Chen
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medicine.medical_specialty ,Survival ,medicine.medical_treatment ,lcsh:Surgery ,Review ,lcsh:RC254-282 ,Standard procedure ,03 medical and health sciences ,0302 clinical medicine ,Postoperative Complications ,Surgical oncology ,Gastrectomy ,Stomach Neoplasms ,medicine ,Humans ,business.industry ,Bursectomy ,Significant difference ,Postoperative complication ,lcsh:RD1-811 ,Advanced gastric cancer ,Length of Stay ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Prognosis ,Surgery ,Meta-analysis ,Oncology ,030220 oncology & carcinogenesis ,Relative risk ,030211 gastroenterology & hepatology ,business ,Gastric cancer - Abstract
Background The present meta-analysis was to explore the surgical and oncological outcomes of bursectomy for advanced gastric cancer (AGC). Methods Relevant studies that evaluated the role of bursectomy for AGC were comprehensively examined to perform a meta-analysis. The primary outcomes were overall survival (OS) and disease-free survival (DFS). The secondary outcomes were the number of harvested lymph nodes (LNs), operation time, operative bleeding, hospital stay, postoperative complication and mortality. Results A total of seven studies comprising 2633 cases (1176 cases in the bursectomy group and 1457 cases in the non-bursectomy group) were finally included. There was no significant difference in OS (HR 0.95, P = 0.647) and DFS (HR 0.99, P = 0.936) between the two groups. Even for patients with serosa-penetrating tumours, OS was comparable between the two groups (HR 0.87, P = 0.356). The operation time of the bursectomy group was longer (weighted mean difference, WMD 32.76 min, P = 0.002). No significant difference was found between the two groups in terms of the number of dissected LNs (WMD 5.86, P = 0.157), operative bleeding (WMD 66.99 ml, P = 0.192) and hospital stay (WMD − 0.15 days, P = 0.766). The overall postoperative complication (relative risk, RR 1.08, P = 0.421) and mortality (RR 0.44, P = 0.195) were similar between two groups. Conclusions This meta-analysis indicated that bursectomy is time-consuming without increasing the number of harvested LNs. Although bursectomy can be safely performed without increasing complications and mortality, it does not prolong the OS and DFS of AGC patients, including patients with serosa-penetrating tumours. Therefore, bursectomy should not be recommended as a standard procedure for AGC. Electronic supplementary material The online version of this article (10.1186/s12957-018-1354-1) contains supplementary material, which is available to authorized users.
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- 2018
28. ALX4, an epigenetically down regulated tumor suppressor, inhibits breast cancer progression by interfering Wnt/β-catenin pathway
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Huidong Zhang, Fei Han, Li Yin, Wen-bin Liu, Hongqiang Chen, Xiang-Lin Hao, Yongsheng Huang, Jun-Tang Yang, Xiao Jiang, Jia Cao, and Jinyi Liu
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0301 basic medicine ,Cancer Research ,CA 15-3 ,Down-Regulation ,Breast Neoplasms ,Biology ,lcsh:RC254-282 ,Metastasis ,Wnt/β-catenin and breast cancer ,Epigenesis, Genetic ,03 medical and health sciences ,Breast cancer ,medicine ,Serine ,Humans ,Wnt Signaling Pathway ,beta Catenin ,ALX4 ,Fluorenes ,DNA methylation ,Cell growth ,Research ,Hydantoins ,Wnt signaling pathway ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,DNA-Binding Proteins ,030104 developmental biology ,Oncology ,Catenin ,Cancer research ,Disease Progression ,Ectopic expression ,Female ,Transcription Factors - Abstract
Background ALX4 is a paired-like homedomain transcription factor mainly expressed in the mesenchymal compartment of variety of developing tissues, but its functions, regulation mechanisms and clinical values in breast cancer remains unclear. Methods The expression of ALX4 in breast cancer cell lines and patients’ tissues were detected by RT-PCR, qPCR and western blot. Furthermore TCGA database was applied to confirm these results. MSP and BSP methods were used to assess the methylation of ALX4 promoter region. In vitro proliferation, metastasis and in vivo nude mice model were used to evaluate the anti-tumor effect of ALX4 on breast cancer cell lines. Luciferase reporter assay, western blot and TCGA database were used to investigate the tumor suppression mechanisms of ALX4. TMA of 142 breast patients was generated to evaluate the clinical significance of ALX4. Results Expression analysis revealed that ALX4 expression is down regulated in breast cancer cell lines and tissues. MSP study showed that the promoter region of ALX4 was hyper-methylated 100% (3/3) in breast cancer cell lines and 69.44% (75/108) in primary breast tumors tissues while 0% (0/8) in normal breast tissues. 5-aza-dc de-methylation treatment restored ALX4 expression in breast cancer cell lines. Functional studies showed that ectopic expression of ALX4 in breast cancer cells inhibited cell proliferation, metastasis in vitro and in vivo. Mechanism study found that ALX4 exerted its anti-tumor function by suppressing the Wnt/β-catenin pathway through promoting the phosphorylation degradation of β-catenin in a GSK3β dependent manner. Clinically multivariate analysis showed that ALX4 expression was an independent favorable prognostic factor in breast cancer patients. Conclusions We reveal for the first time that ALX4 acts as a novel functional tumor suppressor inactivated by DNA methylation and is an independent prognostic factor in breast cancer. Electronic supplementary material The online version of this article (10.1186/s13046-017-0643-9) contains supplementary material, which is available to authorized users.
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- 2017
29. Endoscopic ultrasonography compared with multidetector computed tomography for the preoperative staging of gastric cancer: a meta-analysis
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Li Pu Xu, Shu Qiang Yuan, Xiao Jiang Chen, Yingbo Chen, Shi Chen, Bao Yan Zhu, Zhiwei Zhou, Xiaowei Sun, Yong Ming Chen, and Run Cong Nie
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medicine.medical_specialty ,Staging ,lcsh:Surgery ,Endoscopic ultrasonography ,Review ,lcsh:RC254-282 ,Endosonography ,Multidetector computed tomography ,03 medical and health sciences ,0302 clinical medicine ,Stomach Neoplasms ,Medicine ,Humans ,cardiovascular diseases ,Lymph node ,Neoplasm Staging ,Receiver operating characteristic ,business.industry ,Gastric carcinoma ,Cancer ,lcsh:RD1-811 ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Prognosis ,Confidence interval ,digestive system diseases ,Meta-analysis ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Relative risk ,cardiovascular system ,030211 gastroenterology & hepatology ,Surgery ,Radiology ,business - Abstract
Background The current study sought to perform a meta-analysis to compare the preoperative staging of endoscopic ultrasonography (EUS) and multidetector computed tomography (MDCT) in gastric carcinoma. Methods Articles published between January 1, 2000, and April 1, 2016, that compared EUS with MDCT were included, and data were presented as 2 × 2 tables. The sensitivities, specificities and summary receiver operating characteristic (ROC) curves for T and N staging were calculated using a bivariate mixed effects model. Data were weighted by generic variance and then pooled by random-effects modeling. Results Eight studies comprising 1736 patients were included in this meta-analysis. For T1 staging, the sensitivity value for EUS (82%) was significantly higher than that for MDCT (41%) (relative risk (RR): 2.06, 95% confidence interval (CI) 1.07–3.94; P = 0.030). For lymph node involvement, the sensitivity value for EUS (91%) was also significantly higher than that for MDCT (77%) (RR 1.14, 95% CI 1.05–1.23; P = 0.001). However, the specificity values of both EUS and MDCT were quite low, at 49 and 63%, respectively. No significant differences in T2–4 staging between EUS and MDCT were noted. Conclusion This meta-analysis indicates that EUS may be superior to MDCT in preoperative T1 and N staging. Additionally, the low specificity values of EUS and MDCT for N staging merits attention.
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- 2017
30. Global identification, structural analysis and expression characterization of bHLH transcription factors in wheat
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Xiao-Jiang Guo and Jirui Wang
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0106 biological sciences ,0301 basic medicine ,Subfamily ,genetic processes ,RNA-Seq ,Plant Science ,01 natural sciences ,Synteny ,03 medical and health sciences ,Phylogenetics ,Transcription (biology) ,bHLH ,Arabidopsis ,lcsh:Botany ,Basic Helix-Loop-Helix Transcription Factors ,natural sciences ,Expression pattern ,Transcription factor ,Gene ,Phylogeny ,Triticum ,Plant Proteins ,Genetics ,biology ,Gene Expression Profiling ,fungi ,food and beverages ,biology.organism_classification ,lcsh:QK1-989 ,030104 developmental biology ,Wheat ,Sequence motif ,Genome, Plant ,010606 plant biology & botany ,Research Article - Abstract
Background Basic helix-loop-helix (bHLH) transcription factors (TFs), which are widely distributed in eukaryotic organisms, play crucial roles in plant development. However, no comprehensive analysis of the bHLH family in wheat (Triticum aestivum L.) has been undertaken previously. Results In this study, 225 bHLH TFs predicted from wheat using genomic and RNA sequencing data were subjected to identification, classification, phylogenetic reconstruction, conserved motif characterization, chromosomal distribution determination and expression pattern analysis. One basic region, two helix regions and one loop region were found to be conserved in wheat bHLH TFs. The bHLH proteins could be separated into four categories based on sequences in their basic regions. Neighbor-joining-based phylogenetic analysis of conserved bHLH domains from wheat, Arabidopsis and rice identified 26 subfamilies of bHLH TFs, of which 23 were found in wheat. A total of 82 wheat bHLH genes had orthologs in Arabidopsis (27 TFs), rice (28 TFs) and both of them (27 TFs). Seven tissue-specific bHLH TF clusters were identified according to their expression patterns in endosperm, aleurone, seedlings, heading-stage spikes, flag leaves, shoots and roots. Expression levels of six endosperm-specifically expressed TFs measured by qPCR and RNA-seq showed a good correlation. Conclusion The 225 bHLH transcription factors identified from wheat could be classed into 23 subfamilies, and those members from the same subfamily with similar sequence motifs generally have similar expression patterns. Electronic supplementary material The online version of this article (doi:10.1186/s12870-017-1038-y) contains supplementary material, which is available to authorized users.
- Published
- 2017
31. The role of cage height on the flexibility and load sharing of lumbar spine after lumbar interbody fusion with unilateral and bilateral instrumentation: a biomechanical study.
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Lin Du, Xiao-jiang Sun, Tang-jun Zhou, Yuan-chao Li, Chen Chen, Chang-qing Zhao, Kai Zhang, Jie Zhao, Du, Lin, Sun, Xiao-Jiang, Zhou, Tang-Jun, Li, Yuan-Chao, Chen, Chen, Zhao, Chang-Qing, Zhang, Kai, and Zhao, Jie
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LUMBAR vertebrae surgery , *WEIGHT-bearing (Orthopedics) , *RANGE of motion of joints , *LUMBAR vertebrae , *INTERVERTEBRAL disk - Abstract
Background: One- and two-level lumbar interbody fusion with unilateral instrumentation is as effective as that with bilateral instrumentation. The height of the interbody cage influences the operated segment stability and the fusion technique success. The purpose of this research was to determine the effect of the fusion cage height (i.e. long and short) on both the stability (based on flexibility measures) and load sharing of the unilateral and bilateral instrumented transforaminal lumbar interbody fusion (TLIF) technique.Methods: The flexibility and load sharing tests were performed on seven human lumbar spines. Different configurations combining a long or short cage with a unilateral, bilateral, or no posterior fixation were used to stabilize the operated segment. Two sets of modular cages were designed for each type of test to simulate the long and short cages. During the flexibility test, a pure-moment load of 7.5 Nm was applied. The range of motion (ROM) was recorded for flexion-extension, lateral bending, and axial rotation. During the load sharing test, an axial-compression load of 400 N was applied. The load bearing of the cages was recorded using a cage-embedded load cell.Results: When the fusion cage height decreased 2 mm, the segment flexibility with unilateral fixation showed a significant increase in the ROM for flexion-extension, lateral bending, and axial rotation of 74.9, 83.8, and 175.2% (P < 0.01), respectively. In contrast, for bilateral fixation, the height decrease resulted in no significant change in ROM for flexion-extension (P = 0.686), lateral bending (P = 0.698), and axial rotation (P = 0.133). Using a short fusion cage, the load bearing decreased in 17.1, 21.5, and 54.1% (P < 0.05) for the cage alone, unilateral, and bilateral fixation, respectively.Conclusions: A cage longer than the intervertebral space should be chosen to increase the stability and intervertebral graft load borne when performing TLIF with unilateral instrumentation. [ABSTRACT FROM AUTHOR]- Published
- 2017
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32. Prognostic significance of the total number of harvested lymph nodes for lymph node-negative gastric cancer patients.
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Xin Ji, Zhao-De Bu, Zi-Yu Li, Ai-Wen Wu, Lian-Hai Zhang, Ji Zhang, Xiao-Jiang Wu, Xiang-Long Zong, Shuang-Xi Li, Fei Shan, Zi-Yu Jia, Jia-Fu Ji, Ji, Xin, Bu, Zhao-De, Li, Zi-Yu, Wu, Ai-Wen, Zhang, Lian-Hai, Zhang, Ji, Wu, Xiao-Jiang, and Zong, Xiang-Long
- Subjects
CANCER ,LYMPH nodes ,SURVIVAL ,LYMPH ,LYMPHOCELE ,ANTHROPOMETRY ,GASTRECTOMY ,LONGITUDINAL method ,METASTASIS ,PROGNOSIS ,STOMACH tumors ,SURVIVAL analysis (Biometry) ,TUMOR classification ,TREATMENT effectiveness ,TUMOR grading - Abstract
Background: The relationship between the number of harvested lymph nodes (HLNs) and prognosis of gastric cancer patients without an involvement of lymph nodes has not been well-evaluated. The objective of this study is to further explore this issue.Methods: We collected data from 399 gastric cancer patients between November 2006 and October 2011. All of them were without metastatic lymph nodes.Results: Survival analyses showed that statistically significant differences existed in the survival outcomes between the two groups allocated by the total number of HLNs ranging from 16 to 22. Therefore, we adopted 22 as the cut-off value of the total number of HLNs for grouping (group A: HLNs <22; group B: HLNs≥22). The intraoperative and postoperative characteristics, including operative blood loss (P=0.096), operation time (P=0.430), postoperative hospital stay (P=0.142), complications (P=0.552), rate of reoperation (P=0.966) and postoperative mortality (P=1.000), were comparable between the two groups. T-stage-stratified Kaplan-Meier analyses revealed that the 5-year survival rate of patients at the T4 stage was better in group B than in group A (76.9% vs. 58.5%; P=0.004). An analysis of multiple factors elucidated that the total number of HLNs, T stage, operation time and age were independently correlated factors of prognosis.Conclusions: Regarding gastric cancer patients without the involvement of lymph nodes, an HLN number ≥22 would be helpful in prolonging their overall survival, especially for those at T4 stage. The total number of HLNs was an independent prognostic factor for this population of patients. [ABSTRACT FROM AUTHOR]- Published
- 2017
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33. The optimal extent of gastrectomy for middle-third gastric cancer: distal subtotal gastrectomy is superior to total gastrectomy in short-term effect without sacrificing long-term survival.
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Xin Ji, Yan Yan, Zhao-De Bu, Zi-Yu Li, Ai-Wen Wu, Lian-Hai Zhang, Xiao-Jiang Wu, Xiang-Long Zong, Shuang-Xi Li, Fei Shan, Zi-Yu Jia, Jia-Fu Ji, Ji, Xin, Yan, Yan, Bu, Zhao-De, Li, Zi-Yu, Wu, Ai-Wen, Zhang, Lian-Hai, Wu, Xiao-Jiang, and Zong, Xiang-Long
- Subjects
GASTRECTOMY ,HEALTH outcome assessment ,SURVIVAL analysis (Biometry) ,LYMPH node surgery ,SURGICAL excision ,LYMPH nodes ,STOMACH tumors ,SURGICAL complications ,SURVIVAL ,TIME ,KAPLAN-Meier estimator - Abstract
Background: The optimal extent of gastrectomy for middle-third gastric cancer remains controversial. In our study, the short-term effects and longer-term survival outcomes of distal subtotal gastrectomy and total gastrectomy are analysed to determine the optimal extent of gastrectomy for middle-third gastric cancer.Methods: We retrospectively collect and analyse clinicopathologic data and follow-up outcomes from a prospectively collected database at the Peking University Cancer Hospital. Patients with middle-third gastric adenocarcinoma who underwent curative resection are enrolled in our study.Results: We collect data of 339 patients between January 2005 and October 2011. A total of 144 patients underwent distal subtotal gastrectomy, and 195 patients underwent total gastrectomy. Patients in the total gastrectomy group have longer operative duration (P < 0.001) and postoperative hospital stay (P = 0.001) than those in the distal subtotal gastrectomy group. In the total gastrectomy group, more lymph nodes are harvested (P < 0.001). Meanwhile, the rate of postoperative complications is lower in the distal subtotal gastrectomy group than in the total gastrectomy group (8% vs 15%, P = 0.047). Further analysis demonstrates that the rate of anastomosis leakage is lower in the distal subtotal gastrectomy group than in the total gastrectomy group (0% vs 4%, P = 0.023). Kaplan-Meier (log rank test) analysis shows a significant difference in overall survival between the two groups. The 5-year overall survival rates in the distal subtotal gastrectomy and total gastrectomy groups are 65% and 47%, respectively (P < 0.001). Further stage-stratified analysis reveals that no statistical significance exists in 5-year survival rate between the distal subtotal gastrectomy and total gastrectomy groups at the same stage. Multivariate analysis shows that age (P = 0.046), operation duration (P < 0.001), complications (P = 0.037), usage of neoadjuvant chemotherapy (P < 0.001), tumor size (P = 0.012), presence of lymphovascular invasion (P = 0.043) and N stage (P < 0.001) are independent prognostic factors for survival.Conclusions: For patients with middle-third gastric cancer, distal subtotal gastrectomy shortens the operation duration and postoperative hospital stay and reduces postoperative complications. Meanwhile, the long-term survival of patients with distal subtotal gastrectomy is similar to that of those with total gastrectomy at the same stage. The extent of gastrectomy for middle-third gastric cancer is not an independent prognostic factor for survival. [ABSTRACT FROM AUTHOR]- Published
- 2017
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34. A phase II study of Endostatin in combination with paclitaxel, carboplatin, and radiotherapy in patients with unresectable locally advanced non-small cell lung cancer.
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Xiao-Jiang Sun, Qing-Hua Deng, Xin-Min Yu, Yong-Lin Ji, Yuan-Da Zheng, Hao Jiang, Ya-Ping Xu, Sheng-Lin Ma, Sun, Xiao-Jiang, Deng, Qing-Hua, Yu, Xin-Min, Ji, Yong-Lin, Zheng, Yuan-Da, Jiang, Hao, Xu, Ya-Ping, and Ma, Sheng-Lin
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ENDOSTATIN , *PACLITAXEL , *CARBOPLATIN , *ANTINEOPLASTIC agents , *LUNG cancer , *CLINICAL trials , *COLLAGEN , *COMBINED modality therapy , *COMPARATIVE studies , *RESEARCH methodology , *MEDICAL cooperation , *PROGNOSIS , *RESEARCH , *EVALUATION research , *DISEASE remission - Abstract
Background: Endostatin inhibits the pro-angiogenic action of basic fibroblast growth factor and vascular endothelial growth factor in different human cancers. This study assessed the efficacy of endostatin combined with concurrent chemoradiotherapy of non-small cell lung cancer (NSCLC).Methods: Nineteen patients with unresectable stage III NSCLC, Eastern Cooperative Oncology Group (ECOG) performance status 0-l, and adequate organ function were treated with 60-66 Gy thoracic radiation therapy over 30-33 fractions concurrent with weekly 7.5 mg/m(2) endostatin for 14 days, 50 mg/m(2) paclitaxel, and 2 mg/mL/min carboplatin over 30 min. Patients were then treated with 7.5 mg/m(2) endostatin for 14 days, 150 mg/m(2) paclitaxel, and 5 mg/mL/min carboplatin every 3 weeks for 2 cycles as the consolidation treatment. The objective response rate was recorded according to the Response Evaluation Criteria in Solid Tumors (RECIST) criteria, and the toxicity was evaluated using the National Cancer Institute (NCI) Common Toxicity Criteria.Results: Six patients were unable to complete the consolidation treatment (4 pulmonary toxicity, 1 tracheoesophageal fistulae, and 1 progressive disease). Seventeen patients were included for data analysis. Specifically, one (5.9%) patient had a complete response and 12 (70.6%) had a partial response, whereas two patients had stable disease and the other two had disease progression. The overall response rate was 76% (95% confidence interval [CI], 51%-97%). The median progression-free survival was 10 months (95% CI, 7.6-12.3 months), and the median overall survival was 14 months (95% CI, 10.7-17.2 months). Early 10 patients who completed the treatment regimen showed that four patients experienced grade III pulmonary toxicity a few months after chemoradiotherapy, leading to the early closure of the trial according to the study design.Conclusions: The result of concurrent endostatin treatment with chemoradiotherapy in locally advanced unresectable NSCLC did not meet the goal per study design with unacceptable toxicity. The real impact of endostatin as the first-line treatment combined with chemoradiotherapy on the survival of NSCLC patients remains to be determined. (NCT 01158144). [ABSTRACT FROM AUTHOR]- Published
- 2016
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35. Piperine ameliorates SCA17 neuropathology by reducing ER stress.
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Guo, Jifeng, Cui, Yiting, Liu, Qiong, Yang, Yang, Li, Yujing, Weng, Ling, Tang, Beisha, Jin, Peng, Li, Xiao-Jiang, Yang, Su, and Li, Shihua
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NEUROLOGICAL disorders ,POLYGLUTAMINE ,GLUTAMINE ,ASTROCYTES ,CELL lines - Abstract
Background: Spinocerebellar ataxia 17 (SCA17) belongs to the family of neurodegenerative diseases caused by polyglutamine (polyQ) expansion. In SCA17, polyQ expansion occurs in the TATA box binding protein (TBP) and leads to the misfolding of TBP and the preferential degeneration in the cerebellar Purkinje neurons. Currently there is no effective treatment for SCA17. Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a recently identified neurotrophic factor, and increasing MANF expression ameliorated SCA17 neuropathology in TBP-105Q knock-in (KI) mouse model, indicating that MANF could be a therapeutic target for treating SCA17. Methods: In this study, we screened a collection of 2000 FDA-approved chemicals using a stable cell line expressing luciferase reporter, which is driven by MANF promoter. We identified several potential candidates that can induce the expression of MANF. Of these inducers, piperine is an agent that potently induces the luciferase expression or MANF expression. Results: Addition of piperine in both cellular and mouse models of SCA17 alleviated toxicity caused by mutant TBP. Although mutant TBP is primarily localized in the nuclei, the polyQ expansion in TBP is able to induce ER stress, suggesting that nuclear misfolded proteins can also elicit ER stress as cytoplasmic misfolded proteins do. Moreover, piperine plays its protective role by reducing toxicity caused by the ER stress. Conclusion: Our study established piperine as a MANF-based therapeutic agent for ER stress-related neuropathology in SCA17. [ABSTRACT FROM AUTHOR]
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- 2018
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36. ALX4, an epigenetically down regulated tumor suppressor, inhibits breast cancer progression by interfering Wnt/β-catenin pathway.
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Juntang Yang, Fei Han, Wenbin Liu, Hongqiang Chen, Xianglin Hao, Xiao Jiang, Li Yin, Yongsheng Huang, Jia Cao, Huidong Zhang, and Jinyi Liu
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BREAST cancer ,TUMOR suppressor genes ,HOMEOBOX proteins ,CANCER invasiveness ,WNT genes ,CATENINS - Abstract
Background: ALX4 is a paired-like homedomain transcription factor mainly expressed in the mesenchymal compartment of variety of developing tissues, but its functions, regulation mechanisms and clinical values in breast cancer remains unclear. Methods: The expression of ALX4 in breast cancer cell lines and patients' tissues were detected by RT-PCR, qPCR and western blot. Furthermore TCGA database was applied to confirm these results. MSP and BSP methods were used to assess the methylation of ALX4 promoter region. In vitro proliferation, metastasis and in vivo nude mice model were used to evaluate the anti-tumor effect of ALX4 on breast cancer cell lines. Luciferase reporter assay, western blot and TCGA database were used to investigate the tumor suppression mechanisms of ALX4. TMA of 142 breast patients was generated to evaluate the clinical significance of ALX4. Results: Expression analysis revealed that ALX4 expression is down regulated in breast cancer cell lines and tissues. MSP study showed that the promoter region of ALX4 was hyper-methylated 100% (3/3) in breast cancer cell lines and 69.44% (75/108) in primary breast tumors tissues while 0% (0/8) in normal breast tissues. 5-aza-dc demethylation treatment restored ALX4 expression in breast cancer cell lines. Functional studies showed that ectopic expression of ALX4 in breast cancer cells inhibited cell proliferation, metastasis in vitro and in vivo. Mechanism study found that ALX4 exerted its anti-tumor function by suppressing the Wnt/β-catenin pathway through promoting the phosphorylation degradation of β-catenin in a GSK3β dependent manner. Clinically multivariate analysis showed that ALX4 expression was an independent favorable prognostic factor in breast cancer patients. Conclusions: We reveal for the first time that ALX4 acts as a novel functional tumor suppressor inactivated by DNA methylation and is an independent prognostic factor in breast cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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37. Endoscopic ultrasonography compared with multidetector computed tomography for the preoperative staging of gastric cancer: a meta-analysis.
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Run-Cong Nie, Shu-Qiang Yuan, Xiao-Jiang Chen, Shi Chen, Li-Pu Xu, Yong-Ming Chen, Bao-Yan Zhu, Xiao-Wei Sun, Zhi-Wei Zhou, and Ying-Bo Chen
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ENDOSCOPIC ultrasonography ,MULTIDETECTOR computed tomography ,PREOPERATIVE care ,GASTRIC diseases ,META-analysis - Abstract
Background: The current study sought to perform a meta-analysis to compare the preoperative staging of endoscopic ultrasonography (EUS) and multidetector computed tomography (MDCT) in gastric carcinoma. Methods: Articles published between January 1, 2000, and April 1, 2016, that compared EUS with MDCT were included, and data were presented as 2 × 2 tables. The sensitivities, specificities and summary receiver operating characteristic (ROC) curves for T and N staging were calculated using a bivariate mixed effects model. Data were weighted by generic variance and then pooled by random-effects modeling. Results: Eight studies comprising 1736 patients were included in this meta-analysis. For T1 staging, the sensitivity value for EUS (82%) was significantly higher than that for MDCT (41%) (relative risk (RR): 2.06, 95% confidence interval (CI) 1.07-3.94; P = 0.030). For lymph node involvement, the sensitivity value for EUS (91%) was also significantly higher than that for MDCT (77%) (RR 1.14, 95% CI 1.05-1.23; P = 0.001). However, the specificity values of both EUS and MDCT were quite low, at 49 and 63%, respectively. No significant differences in T2-4 staging between EUS and MDCT were noted. Conclusion: This meta-analysis indicates that EUS may be superior to MDCT in preoperative T1 and N staging. Additionally, the low specificity values of EUS and MDCT for N staging merits attention. [ABSTRACT FROM AUTHOR]
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- 2017
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38. Elevated transcriptional levels of aldolase A (ALDOA) associates with cell cycle-related genes in patients with NSCLC and several solid tumors.
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Fan Zhang, Jie-Diao Lin, Xiao-Yu Zuo, Yi-Xuan Zhuang, Chao-Qun Hong, Guo-Jun Zhang, Xiao-Jiang Cui, and Yu-Kun Cui
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BREAST cancer ,CANCER ,CELL cycle ,ALDOLASES ,GLYCOLYSIS ,ENZYMES ,TUMORS ,DNA - Abstract
Background: Aldolase A (ALDOA) is one of the glycolytic enzymes primarily found in the developing embryo and adult muscle. Recently, a new role of ALDOA in several cancers has been proposed. However, the underlying mechanism remains obscure and inconsistent. In this study, we tried to investigate ALDOA-associated (AA) genes using available microarray datasets to help elucidating the role of ALDOA in cancer. Results: In the dataset of patients with non-small-cell lung cancer (NSCLC, E-GEOD- 19188), 3448 differentially expressed genes (DEGs) including ALDOA were identified, in which 710 AA genes were found to be positively associated with ALDOA. Then according to correlation coefficients between each pair of AA genes, ALDOA-associated gene co-expression network (GCN) was constructed including 182 nodes and 1619 edges. 11 clusters out of GCN were detected by ClusterOne plugin in Cytoscape, and only 3 of them havemore than three nodes. These three clusters were functionally enriched. A great number of genes (43/79, 54.4%) in the biggest cluster (Cluster 1) primarily involved in biological process like cell cycle process (P
a = 6.76E-26), mitotic cell cycle (Pa = 4.09E-19), DNA repair (Pa = 1.13E-04), M phase of meiotic cell cycle (Pa = 0.006), positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle (Pa = 0.014). AA genes with highest degree and betweenness were considered as hub genes of GCN, namely CDC20, MELK, PTTG1, CCNB2, CDC45, CCNB1, TK1 and PSMB2, which could distinguish cancer from normal controls with ALDOA. Their positive association with ALDOA remained after removing the effect of HK2 and PKM, the two rate limiting enzymes in glycolysis. Further, knocking down ALDOA blocked breast cancer cells in the G0/G1 phase under minimized glycolysis. All suggested that ALDOA might affect cell cycle progression independent of glycolysis. RT-qPCR detection confirmed the relationship of ALDOA with CDC45 and CCNB2 in breast tumors. High expression of the hub genes indicated poor outcome in NSCLC. ALDOA could improve their predictive power. Conclusions: ALDOA could contribute to the progress of cancer, at least partially through its association with genes relevant to cell cycle independent of glycolysis. AA genes plus ALDOA represent a potential new signature for development and prognosis in several cancers. [ABSTRACT FROM AUTHOR]- Published
- 2017
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39. Comparison of different methods of splenic hilar lymph node dissection for advanced upper- and/or middle-third gastric cancer.
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Xin Ji, Tao Fu, Zhao-De Bu, Ji Zhang, Xiao-Jiang Wu, Xiang-Long Zong, Zi-Yu Jia, Biao Fan, Yi-Nan Zhang, and Jia-Fu Ji
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STOMACH cancer treatment ,LYMPHADENECTOMY ,COMPARATIVE studies ,HEALTH surveys ,CANCER chemotherapy ,ADJUVANT treatment of cancer ,RETROSPECTIVE studies - Abstract
Background: Surgery for advanced gastric cancer (AGC) often includes dissection of splenic hilar lymph nodes (SHLNs). This study compared the safety and effectiveness of different approaches to SHLN dissection for upperand/ or middle-third AGC. Methods: We retrospectively compared and analyzed clinicopathologic and follow-up data from a prospectively collected database at the Peking University Cancer Hospital. Patients were divided into three groups: in situ spleenpreserved, ex situ spleen-preserved and splenectomy. Results: We analyzed 217 patients with upper- and/or middle-third AGC who underwent R0 total or proximal gastrectomy with splenic hilar lymphadenectomy from January 2006 to December 2011, of whom 15.2% (33/ 217) had metastatic SHLNs, and from whom 11.4% (53/466) of the dissected SHLNs were metastatic. The number of harvested SHLNs per patient was higher in the ex situ group than in the in situ group (P = 0.017). Length of postoperative hospital stay was longer in the splenectomy group than in the in situ group (P = 0.002) or the ex situ group (P < 0.001). The splenectomy group also lost more blood volume (P = 0.007) and had a higher postoperative complication rate (P = 0.005) than the ex situ group. Kaplan-Meier (log rank test) analysis showed significant survival differences among the three groups (P = 0.018). Multivariate analysis showed operation duration (P = 0.043), blood loss volume (P = 0.046), neoadjuvant chemotherapy (P = 0.005), and N stage (P < 0.001) were independent prognostic factors for survival. Conclusions: The ex situ procedure was more effective for SHLN dissection than the in situ procedure without sacrificing safety, whereas splenectomy was not more effective, and was less safe. The SHLN dissection method was not an independent risk factor for survival in this study. [ABSTRACT FROM AUTHOR]
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- 2016
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40. Fast track multi-discipline treatment (FTMDT trial) versus conventional treatment in colorectal cancer--the design of a prospective randomized controlled study
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Min Yan, Yong-Mao Song, Jiaojiao Zhou, Xiao-Jiang Ying, Rong Chen, Gang Chen, Ke-Feng Ding, and Jun Li
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Laparoscopic surgery ,medicine.medical_specialty ,Cancer Research ,Oxaloacetates ,Colorectal cancer ,medicine.medical_treatment ,Leucovorin ,lcsh:RC254-282 ,Deoxycytidine ,Colorectal neoplasms ,law.invention ,Study Protocol ,Randomized controlled trial ,Clinical Protocols ,law ,Colorectal surgery ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Clinical endpoint ,Genetics ,Humans ,Prospective Studies ,Prospective cohort study ,Capecitabine ,business.industry ,Rehabilitation ,Perioperative ,Length of Stay ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Surgery ,Hospitalization ,Oncology ,Laparoscopy ,Fluorouracil ,Fast track ,business - Abstract
Background Laparoscopy-assisted surgery, fast-track perioperative treatment are both increasingly used in colorectal cancer treatment, for their short-time benefits of enhanced recovery and short hospital stays. However, the benefits of the integration of the Laparoscopy-assisted surgery, fast-track perioperative treatment, and even with the Xelox chemotherapy, are still unknown. In this study, the three treatments integration is defined as "Fast Track Multi-Discipline Treatment Model" for colorectal cancer and this model extends the benefits to the whole treatment process of colorectal cancer. The main purpose of the study is to explore the feasibility of "Fast Track Multi-Discipline Treatment" model in treatment of colorectal cancer. Methods The trial is a prospective randomized controlled study with 2 × 2 balanced factorial design. Patients eligible for the study will be randomized to 4 groups: (I) Laparoscopic surgery with fast track perioperative treatment and Xelox chemotherapy; (II) Open surgery with fast track perioperative treatment and Xelox chemotherapy; (III) Laparoscopic surgery with conventional perioperative treatment and mFolfox6 chemotherapy; (IV) Open surgery with conventional perioperative treatment and mFolfox6 chemotherapy. The primary endpoint of this study is the hospital stays. The secondary endpoints are the quality of life, chemotherapy related adverse events, surgical complications and hospitalization costs. Totally, 340 patients will be enrolled with 85 patients in each group. Conclusions The study initiates a new treatment model "Fast Track Multi-Discipline Treatment" for colorectal cancer, and will provide feasibility evidence on the new model "Fast Track Multi-Discipline Treatment" for patients with colorectal cancer. Trial registration ClinicalTrials.gov: NCT01080547
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- 2011
41. Human leukocyte antigen (HLA) class I frequencies in human T-cell lymphotropic virus type 1 (HTLV-1)-infected patients from Salvador-Brazil
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Alline Nascimento, Maria Fernanda Rios Grassi, Viviana Olavarria, Ramon de Almeida Kruschewsky, Mary Carrigton, Xiao-Jiang Gao, Bernardo Galvão-Castro, and David I. Watkins
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lcsh:Immunologic diseases. Allergy ,biology ,business.industry ,viruses ,Cell ,virus diseases ,Human leukocyte antigen ,medicine.disease ,Virology ,Myelopathy ,Infectious Diseases ,medicine.anatomical_structure ,immune system diseases ,Virus type ,Infectious disease (medical specialty) ,Immunology ,Tropical spastic paraparesis ,Meeting Abstract ,biology.protein ,Medicine ,Human T cell lymphotropic virus type 1 ,Antibody ,business ,lcsh:RC581-607 - Abstract
The development of human T- cell lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) may be related to genetic factors related to the presentation of viral antigens by human leukocyte antigen (HLA).
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- 2011
42. Cytoplasmic mislocalization of RNA splicing factors and aberrant neuronal gene splicing in TDP-43 transgenic pig brain.
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Guohao Wang, Huaqiang Yang, Sen Yan, Chuan-En Wang, Xudong Liu, Bentian Zhao, Zhen Ouyang, Peng Yin, Zhaoming Liu, Yu Zhao, Tao Liu, Nana Fan, Lin Guo, Shihua Li, Xiao-Jiang Li, and Liangxue Lai
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DNA-binding proteins ,CYTOPLASM ,AMYOTROPHIC lateral sclerosis ,FRONTOTEMPORAL lobar degeneration ,TRANSGENIC animals - Abstract
Background: TAR DNA-binding protein 43 (TDP-43) is a nuclear protein, but it is redistributed in the neuronal cytoplasm in both amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Because small transgenic animal models often lack cytoplasmic TDP-43, how the cytoplasmic accumulation of TDP-43 contributes to these diseases remains unclear. The current study is aimed at studying the mechanism of cytoplasmic pathology of TDP-43. Results: We established transgenic pigs expressing mutant TDP-43 (M337V). This pig model shows severe phenotypes and early death. We found that transgenic TDP-43 is also distributed in the cytoplasm of neuronal cells in the spinal cord and brain. Transgenic TDP-43 interacts with PSF, an RNA splicing factor that associates with NeuN to regulate neuronal RNA splicing. The interaction of TDP-43, PSF and NeuN causes PSF and NeuN mislocalize into the neuronal cytoplasm in transgenic pigs. Consistently, abnormal PSF-related neuronal RNA splicing is seen in TDP-43 transgenic pigs. The cytoplasmic localization of PSF and NeuN as well as abnormal PSF-related neuronal RNA splicing was also found in ALS patient brains. Conclusion: Our findings from a large mammalian model suggest that cytoplasmic mutant TDP-43 could reduce the nuclear function of RNA splicing factors, contributing to neuropathology. [ABSTRACT FROM AUTHOR]
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- 2015
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43. CRISPR/Cas9: a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases.
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Zhuchi Tu, Weili Yang, Sen Yan, Xiangyu Guo, and Xiao-Jiang Li
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NEURODEGENERATION ,ANIMAL models in research ,BIOTECHNOLOGY ,GENETIC engineering ,BIOETHICS ,GENETIC recombination ,TRANSGENIC organisms - Abstract
Animal models are extremely valuable to help us understand the pathogenesis of neurodegenerative disorders and to find treatments for them. Since large animals are more like humans than rodents, they make good models to identify the important pathological events that may be seen in humans but not in small animals; large animals are also very important for validating effective treatments or confirming therapeutic targets. Due to the lack of embryonic stem cell lines from large animals, it has been difficult to use traditional gene targeting technology to establish large animal models of neurodegenerative diseases. Recently, CRISPR/Cas9 was used successfully to genetically modify genomes in various species. Here we discuss the use of CRISPR/Cas9 technology to establish large animal models that can more faithfully mimic human neurodegenerative diseases. [ABSTRACT FROM AUTHOR]
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- 2015
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44. Elevated C1orf63 expression is correlated with CDK10 and predicts better outcome for advanced breast cancers: a retrospective study.
- Author
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Chao-Qun Hong, Fan Zhang, Yan-Jie You, Wei-Li Qiu, Giuliano, Armando E., Xiao-Jiang Cui, Guo-Jun Zhang, and Yu-Kun Cui
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BREAST cancer treatment ,GENE expression ,CYCLIN-dependent kinases ,BREAST cancer patients ,RETROSPECTIVE studies ,STATISTICAL correlation - Abstract
Background: Chromosome 1 open reading frame 63 (C1orf63) is located on the distal short arm of chromosome 1, whose allelic loss has been observed in several human cancers. C1orf63 has been reported to be up-regulated in IL-2-starved T lymphocytes, which suggests it might be involved in cell cycle control, a common mechanism for carcinogenesis. Here we investigated the expression and clinical implication of C1orf63 in breast cancer. Methods: Paraffin-embedded specimens, clinicopathological features and follow-up data of the breast cancer patients were collected. Publicly available microarray and RNA-seq datasets used in this study were downloaded from ArrayExpress of EBI and GEO of NCBI. KM plotter tool was also adopted. The expression of C1orf63 and CDK10, one known cell cycle-dependent tumor suppressor in breast cancer, was assessed by immunohistochemistry. Western blotting was performed to detect C1orf63 protein in human breast cancer cell lines, purchased from the Culture Collection of the Chinese Academy of Sciences, Shanghai. Results: In a group of 12 human breast tumors and their matched adjacent non-cancerous tissues, C1orf63 expression was observed in 7 of the 12 breast tumors, but not in the 12 adjacent non-cancerous tissues (P < 0.001). Similar results were observed of C1orf63 mRNA expression both in breast cancer and several other cancers, including lung cancer, prostate cancer and hepatocellular carcinoma. In another group of 182 breast cancer patients, C1orf63 expression in tumors was not correlated with any clinicopathological features collected in this study. Survival analyses showed that there was no significant difference of overall survival (OS) rates between the C1orf63 (+) group and the C1orf63 (-) group (P = 0.145). However, the analyses of KM plotter displayed a valid relationship between C1orf63 and RFS (relapse free survival)/OS (P < 0.001; P = 0.007). Notablely, in breast cancers with advanced TNM stages (III ~ IV) among these 182 patients, C1orf63 expression was an independent prognostic factor predicting better clinical outcome (HR: 0.41; 95 % CI: 0.17 ~ 0.97; P = 0.042). Additionally, we found that CDK10 mRNA expression was positively correlated with C1orf63, which was consistent with the relationship of protein expression between C1orf63 and CDK10 (r
s = 0.391; P < 0.001). Conclusions: Compared to adjacent non-cancerous tissues, C1orf63 expression was elevated in tumor tissues. However, C1orf63 predicts better prognosis for breast cancers with advanced TNM stage, and the underlying mechanism is unknown. In addition, C1orf63 is correlated with the cell cycle related gene, CDK10. [ABSTRACT FROM AUTHOR]- Published
- 2015
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45. Neoadjuvant chemoradiation therapy for resectable esophago-gastric adenocarcinoma: a meta-analysis of randomized clinical trials.
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Tao Fu, Zhao-De Bu, Zi-Yu Li, Lian-Hai Zhang, Xiao-Jiang Wu, Ai-Wen Wu, Fei Shan, Xin Ji, Qiu-Shi Dong, and Jia-Fu Ji
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ESOPHAGOGASTRIC junction cancer ,SURGICAL excision ,METASTASIS ,CANCER relapse ,CLINICAL trials - Abstract
Background: The efficacy and safety of preoperative chemoradiation therapy (CRT) for advanced esophago-gastric adenocarcinoma are still in question, and the prognosis of these patients is poor. Methods: We systematically searched electronic databases from January 1990 to July 2014. The primary outcome was overall survival. The secondary outcomes were a R0 resection rate, positive rate of lymph node metastasis, postoperative recurrence rate, pathological complete response (pCR) rate and perioperative mortality. Overall survival was measured with a hazard ratio (HR), while other secondary outcomes were measured with an odds ratio (OR). Results: Seven randomized controlled trials (RCTs) including 1085 patients were searched and, of these, 869 had adenocarcinoma. Patients receiving preoperative CRT had a longer overall survival (HR 0.74; 95% confidence interval (CI) 0.63-0.88), higher likelihood of R0 resection and greater chance of pCR, while they had a lower likelihood of lymph node metastasis and postoperative recurrence. The difference of perioperative mortality was non-significant. In addition, the result of the comparison between preoperative CRT and preoperative chemotherapy (CT) in two RCTs was non-significant. Conclusion: Patients with resectable esophago-gastric adenocarcinoma can gain a survival advantage from preoperative CRT. However, limited to the number of RCTs, the effect of adding radiotherapy to preoperative CT separately is still uncertain and more high-quality prospective trials are needed. [ABSTRACT FROM AUTHOR]
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- 2015
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46. Retrospective analysis of individual risk factors for urethrocutaneous fistula after onlay hypospadias repair in pediatric patients.
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Li-Qu Huang, Zheng Ge, Jun Tian, Geng Ma, Ru-Gang Lu, Yong-Ji Deng, Li-Xia Wang, Chen-Jun Chen, Hao-Bo Zhu, Xiao-Jiang Zhu, and Yun-Fei Guo
- Subjects
SKIN diseases ,FISTULA ,URINARY fistula ,AGE distribution ,CONFIDENCE intervals ,UROLOGICAL surgery ,HYPOSPADIAS ,MEN ,MULTIVARIATE analysis ,RESEARCH funding ,SURGICAL complications ,LOGISTIC regression analysis ,RETROSPECTIVE studies ,DATA analysis software ,DESCRIPTIVE statistics ,ODDS ratio ,DISEASE risk factors - Abstract
Background: To retrospectively identify the individual risk factors for the urethrocutaneous fistula (UCF) in pediatric patients after hypospadias repair (HR) with onlay island flap urethroplasty. Methods: A total of 167 patients who underwent primary HR at Nanjing Medical University Affiliated Children Hospital from January 2009 to December 2012 were enrolled. Clinical data including the patient' age at HR, hypospadias type and urethral defect length were documented. Results: Among 167 patients, 12.6% patients (n = 21) developed UCF after HR. Postoperative UCF occurred in 3.9% (3/76) cases at age of 0-2 years, 14.3% (9/63) at 2-4 years, 20.0% (2/10) at 4-6 years and 38.9% (7/18) at 6-12 years. The incidences of UCF were 12.0% (3/25), 11.4% (5/132) and 30.0% (3/10) for distal, middle and proximal types of hypospadias. As to the urethral defect length, the incidences of UCF were 8.2% (5/61) in patients with a length of = 2 cm, 12.8% (9/70) in 2-3 cm, 22.6% (7/31) in 3-4 cm and 0% (0/5) in above 4 cm. Older age at HR was significantly associated with the high incidence of UCF formation (P = 0.004), while the hypospadias type and urethral defect length did not affect it (P =0 . 2 6 4a n d P = 0.312, respectively). Conclusions: The patient' age at HR was a risk factor for the UCF formation after HR, and treatment of HR within two years old might be with the least incidence of UCF. [ABSTRACT FROM AUTHOR]
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- 2015
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47. Genome-wide characterization of developmental stage- and tissue-specific transcription factors in wheat.
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Zhen-Yong Chen, Xiao-Jiang Guo, Zhong-Xu Chen, Wei-Ying Chen, Deng-Cai Liu, You-Liang Zheng, Ya-Xi Liu, Yu-Ming Wei, and Ji-Rui Wang
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DEVELOPMENTAL biology , *GENE expression , *GENOMICS , *TISSUE analysis , *DNA ,WHEAT genetics - Abstract
Background: Wheat (Triticum aestivum) is one of the most important cereal crops, providing food for humans and feed for other animals. However, its productivity is challenged by various biotic and abiotic stresses such as fungal diseases, insects, drought, salinity, and cold. Transcription factors (TFs) regulate gene expression in different tissues and at various developmental stages in plants and animals, and they can be identified and classified into families according to their structural and specialized DNA-binding domains (DBDs). Transcription factors are important regulatory components of the genome, and are the main targets for engineering stress tolerance. Results: In total, 2407 putative TFs were identified from wheat expressed sequence tags, and then classified into 63 families by using Hmm searches against hidden Markov model (HMM) profiles. In this study, 2407 TFs represented approximately 2.22% of all genes in the wheat genome, a smaller proportion than those reported for other cereals in PlantTFDB V3.0 (3.33%-5.86%) and PlnTFDB (4.30%-6.46%). We assembled information from the various databases for individual TFs, including annotations and details of their developmental stage- and tissue-specific expression patterns. Based on this information, we identified 1257 developmental stage-specific TFs and 1104 tissue-specific TFs, accounting for 52.22% and 45.87% of the 2407 wheat TFs, respectively. We identified 338, 269, 262, 175, 49, and 18 tissue-specific TFs in the flower, seed, root, leaf, stem, and crown, respectively. There were 100, 6, 342, 141, 390, and 278 TFs specifically expressed at the dormant seed, germinating seed, reproductive, ripening, seedling, and vegetative stages, respectively. We constructed a comprehensive database of wheat TFs, designated as WheatTFDB (http://xms.sicau.edu.cn/wheatTFDB/). Conclusions: Approximately 2.22% (2407 genes) of all genes in the wheat genome were identified as TFs, and were clustered into 63 TF families. We identified 1257 developmental stage-specific TFs and 1104 tissue-specific TFs, based on information about their developmental- and tissue-specific expression patterns obtained from publicly available gene expression databases. The 2407 wheat TFs and their annotations are summarized in our database, WheatTFDB. These data will be useful identifying target TFs involved in the stress response at a particular stage of development. [ABSTRACT FROM AUTHOR]
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- 2015
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48. Pathological cell-cell interactions are necessary for striatal pathogenesis in a conditional mouse model of Huntington's disease
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Shihua Li, X. William Yang, Carlos Cepeda, Michael Levine, Xiaofeng Gu, Véronique M. André, and Xiao-Jiang Li
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Cell type ,Huntingtin ,Clinical Neurology ,Neuropathology ,lcsh:Geriatrics ,medicine.disease ,Medium spiny neuron ,lcsh:RC346-429 ,Pathogenesis ,lcsh:RC952-954.6 ,Cellular and Molecular Neuroscience ,Huntington's disease ,Gliosis ,nervous system ,medicine ,Neurology (clinical) ,medicine.symptom ,Psychology ,Receptor ,Molecular Biology ,Neuroscience ,lcsh:Neurology. Diseases of the nervous system ,Research Article - Abstract
A critical issue in understanding Huntington's disease (HD) pathogenesis is how the ubiquitously expressed mutant huntingtin (mhtt) with an expanded polyglutamine repeat can cause selective toxicity of striatal and cortical neurons. Two potential cellular models may contribute to such specificity: expression of mhtt in these vulnerable neurons alone may be sufficient to result in their dysfunction and/or degeneration (cell-autonomous model); or mhtt in other cell types can elicit pathological cell-cell interactions to cause the vulnerable neurons to become dysfunctional and be at risk for degeneration (cell-cell interaction model). To distinguish between these two models, we have selectively expressed a neuropathogenic fragment of mhtt-exon1 in striatal medium spiny neurons (MSNs) by crossing a conditional mouse model of HD with a striatal-specific Cre mouse line. In this striatal model of HD, we observed progressive and cell-autonomous nuclear accumulation of mhtt aggregates in MSNs. Surprisingly, unlike the mouse model expressing mhtt-exon1 in all the neurons in the brain, the striatal model lacks significant locomotor deficits and striatal neuropathology including gliosis and dark degenerating neurons. Electrophysiological findings from acutely dissociated MSNs revealed a cell-autonomous deficit in N-methyl-d-aspartate (NMDA) receptor sensitivity to Mg2+, a deficit also present in other mouse models of HD. In conclusion, this study provides the first in vivo genetic evidence that pathological cell-cell interactions are necessary for striatal pathogenesis in a conditional mouse model of HD, and suggests a ''two-hit'' hypothesis in which both cell-autonomous toxicity and pathological cell-cell interactions are critical to HD pathogenesis.
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- 2007
49. Longitudinal transcriptomic dysregulation in the peripheral blood of transgenic Huntington's disease monkeys.
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Kocerha, Jannet, Yuhong Liu, Willoughby, David, Chidamparam, Kumaravel, Benito, Joseph, Nelson, Kate, Xu, Yan, Tim Chi, Engelhardt, Heidi, Moran, Sean, Shang-Hsun Yang, Shi-Hua Li, Xiao-Jiang Li, Larkin, Katherine, Neumann, Adam, Banta, Heather, Jin Jing Yang, and Chan, Anthony W. S.
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HUNTINGTON disease ,GENETIC disorders ,NEURODEGENERATION ,POLYGLUTAMINE ,GENETIC transcription regulation ,GENETICS - Abstract
Background: Huntington's Disease (HD) is a progressive neurodegenerative disorder caused by an expansion in the polyglutamine (polyQ) region of the Huntingtin (HTT) gene. The clinical features of HD are characterized by cognitive, psychological, and motor deficits. Molecular instability, a core component in neurological disease progression, can be comprehensively evaluated through longitudinal transcriptomic profiling. Development of animal models amenable to longitudinal examination enables distinct disease-associated mechanisms to be identified. Results: Here we report the first longitudinal study of transgenic monkeys with genomic integration of various lengths of the human HTT gene and a range of polyQ repeats. With this unique group of transgenic HD nonhuman primates (HD monkeys), we profiled over 47,000 transcripts from peripheral blood collected over a 2 year timespan from HD monkeys and age-matched wild-type control monkeys. Conclusions: Messenger RNAs with expression patterns which diverged with disease progression in the HD monkeys considerably facilitated our search for transcripts with diagnostic or therapeutic potential in the blood of human HD patients, opening up a new avenue for clinical investigation. [ABSTRACT FROM AUTHOR]
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- 2013
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50. Quantitative T2 mapping to characterize the process of intervertebral disc degeneration in a rabbit model.
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Wei Sun, Kai Zhang, Chang-qing Zhao, Wei Ding, Jun-jie Yuan, Qi Sun, Xiao-jiang Sun, You-zhuan Xie, Hua Li, and Jie Zhao
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GENE mapping ,INTERVERTEBRAL disk diseases ,LABORATORY rabbits ,ANIMAL disease models ,POLYMERASE chain reaction ,AGGRECAN ,COLLAGEN genetics ,NUCLEUS pulposus - Abstract
Background To investigate the potential of T2 mapping for characterizing the process of intervertebral disc degeneration (IDD) in a rabbit model. Methods Thirty-five rabbits underwent an annular stab to the L4/5 discs (L5/6 discs served as internal normal controls). Degenerative changes were graded according to the modified Thompson classification and quantified in T2 respectively at pre-operation, 1, 3, 6, 12 and 24 weeks postoperatively. After MRI analysis, expression analysis of aggrecan and type II collagen gene in nucleus pulposus (NP) was performed using real time polymerase chain reaction (real-time PCR). The longitudinal changes in NP T2 and gene expressions were studied by repeated measures and ANOVA, linear regression was performed for their correlations through the process of IDD. The reliability analysis of method of measurement of NP T2 was also performed. Results There was a strong inverse correlation between NP T2 and Thompson grades (r = -0.85). The decline of L4/5 NP T2 through 24 weeks was nonlinear, the most significant decrease was observed in 3 weeks postoperatively (P<0.05). The tendency was confirmed at gene expression levels. NP T2 correlated strongly with aggrecan (R² = 0.85, P<0.01) and type II collagen (R² = 0.78, P<0.01) gene expressions. The intraclass correlation coefficients for interobserver and intraobserver reliability were 0.963 and 0.977 respectively. Conclusions NP T2 correlates well with aggrecan and type II collagen gene expressions. T2 mapping could act as a sensitive, noninvasive tool for quantitatively characterizing the process of IDD in longitudinal study, help better understanding of the pathophysiology of IDD, assist us to detect the degenerative cascade, and develop a T2-based quantification scale for evaluation of IDD and efficacy of therapeutic interventions. [ABSTRACT FROM AUTHOR]
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- 2013
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