6,612 results on '"WANG, X."'
Search Results
2. Validation of the International Trauma Questionnaire—Child and Adolescent Version (ITQ-CA) in a Chinese mental health service seeking adolescent sample
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Ho, G. W. K., Liu, H., Karatzias, T., Hyland, P., Cloitre, M., Lueger-Schuster, B., Brewin, C. R., Guo, C., Wang, X., and Shevlin, M.
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- 2022
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3. Polyphosphate (PolyP) for alveolar cleft repair: study protocol for a pilot randomized controlled trial
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Alkaabi, S. A., Natsir Kalla, D. S., Alsabri, G. A., Fauzi, A., Tajrin, A., Müller, W. E. G., Schröder, H. C., Wang, X. G., Forouzanfar, T., Helder, M. N., and Ruslin, M.
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- 2021
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4. Informing the public health response to COVID-19: a systematic review of risk factors for disease, severity, and mortality
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Flook, M., Jackson, C., Vasileiou, E., Simpson, C. R., Muckian, M. D., Agrawal, U., McCowan, C., Jia, Y., Murray, J. L. K., Ritchie, L. D., Robertson, C., Stock, S. J., Wang, X., Woolhouse, M. E. J., Sheikh, A., and Stagg, H. R.
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- 2021
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5. Acceptability and practicability of self-management for patients with Parkinson’s disease based on smartphone applications in China
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Hu, J., Yuan, D. Z., Zhao, Q. Y., Wang, X. F., Zhang, X. T., Jiang, Q. H., Luo, H. R., Li, J., Ran, J. H., and Li, J. F.
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- 2020
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6. How does the two-child policy affect the sex ratio at birth in China? A cross-sectional study
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Fan, S. L., Xiao, C. N., Zhang, Y. K., Li, Y. L., Wang, X. L., and Wang, L.
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- 2020
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7. A comprehensive study of risk factors for post-operative pneumonia following resection of meningioma
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Zuo, M. R., Liang, R. F., Li, M., Xiang, Y. F., Zhang, S. X., Yang, Y., Wang, X., Mao, Q., and Liu, Y. H.
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- 2019
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8. Which cancer survivors are at risk for a physically inactive and sedentary lifestyle? Results from pooled accelerometer data of 1447 cancer survivors
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Sweegers, M. G., Boyle, T., Vallance, J. K., Chinapaw, M. J., Brug, J., Aaronson, N. K., D’Silva, A., Kampshoff, C. S., Lynch, B. M., Nollet, F., Phillips, S. M., Stuiver, M. M., van Waart, H., Wang, X., Buffart, L. M., and Altenburg, T. M.
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- 2019
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9. The effects of ALDH2 Glu487Lys polymorphism on vasovagal syncope patients undergoing head-up tilt test supplemented with sublingual nitroglycerin.
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Xia, G, Jin, JF, Ye, Y, Wang, XD, Hu, B, Pu, JL, Jin, J F, Wang, X D, and Pu, J L
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SYNCOPE ,SUBLINGUAL drug administration ,TILT-table test ,EAST Asians ,ALDEHYDE dehydrogenase ,NITROGLYCERIN - Abstract
Background and Objective: Head-up tilt test (HUTT) is clinically advantageous for diagnosing patients with vasovagal syncope (VVS). Nitroglycerin is mainly used as a stimulant during HUTT, and mitochondrial aldehyde dehydrogenase 2 (ALDH2) is involved in the metabolism of nitroglycerin (NTG). ALDH2 Glu487Lys polymorphism (ALDH2 rs671) is the most common variant in the East Asian population. This study aimed to assess the effects of ALDH2 rs671 on VVS patients undergoing HUTT supplemented with sublingual NTG (HUTT-NTG). METHODS: Patients with recurrent VVS (at least 2 times) who were admitted to the syncope center of our hospital were enrolled. All VVS patients have undergone HUTT. The polymorphism of Glu487Lys gene of ALDH2 was measured by the DNA Microarray Chip Method. The results of HUTT-NTG of VVS patients with different ALDH2 genotypes were compared and their hemodynamic characteristics were assessed.Results: A total of 199 VVS patients were enrolled, including 101 patients in the ALDH2*1/*1 group and 98 patients in the ALDH2*2 group. Among patients undergoing HUTT-NTG, 70.3% of patients in the ALDH2*1/*1 group and 68.4% of patients in the ALDH2*2 group were positive, and the difference between the two groups was not statistically significant (P = 0.77). The proportions of VASIS I, VASIS II, and VASIS III were 40.6%, 8.9%, and 20.8% in the ALDH2*1/*1 group, respectively, and the corresponding proportions in the ALDH2*2 group were 36.7%, 11.2%, and 20.4%, respectively. There was no statistically significant difference between the two groups (P = 0.91). The hemodynamic characteristics of different genotypes in VVS patients undergoing HUTT-NTG were compared, and no statistically significant difference was found. The median time of syncopal episode occurred after NTG administration in the ALDH2*1/*1 group was 6 min (interquartile range [IQR]: 5.0-9.0), and it was 6.0 min in the ALDH2*2 group (IQR: 4.25-8.0, P = 0.64).Conclusion: ALDH2 Glu487Lys polymorphism did not affect the outcome of VVS patients undergoing HUTT-NTG, and no significant change in the hemodynamic characteristics of different genotypes was found. [ABSTRACT FROM AUTHOR]- Published
- 2022
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10. The role of Sox6 in zebrafish muscle fiber type specification
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Jackson, H.E., Ono, Y., Wang, X., Elworthy, S., Cunliffe, V.T., and Ingham, P.W.
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Background\ud The transcription factor Sox6 has been implicated in regulating muscle fiber type-specific gene expression in mammals. In zebrafish, loss of function of the transcription factor Prdm1a results in a slow to fast-twitch fiber type transformation presaged by ectopic expression of sox6 in slow-twitch progenitors. Morpholino-mediated Sox6 knockdown can suppress this transformation but causes ectopic expression of only one of three slow-twitch specific genes assayed. Here, we use gain and loss of function analysis to analyse further the role of Sox6 in zebrafish muscle fiber type specification.\ud \ud Methods\ud The GAL4 binary misexpression system was used to express Sox6 ectopically in zebrafish embryos. Cis-regulatory elements were characterized using transgenic fish. Zinc finger nuclease mediated targeted mutagenesis was used to analyse the effects of loss of Sox6 function in embryonic, larval and adult zebrafish. Zebrafish transgenic for the GCaMP3 Calcium reporter were used to assay Ca2+ transients in wild-type and mutant muscle fibres.\ud \ud Results\ud Ectopic Sox6 expression is sufficient to downregulate slow-twitch specific gene expression in zebrafish embryos. Cis-regulatory elements upstream of the slow myosin heavy chain 1 (smyhc1) and slow troponin c (tnnc1b) genes contain putative Sox6 binding sites required for repression of the former but not the latter. Embryos homozygous for sox6 null alleles expressed tnnc1b throughout the fast-twitch muscle whereas other slow-specific muscle genes, including smyhc1, were expressed ectopically in only a subset of fast-twitch fibers. Ca2+ transients in sox6 mutant fast-twitch fibers were intermediate in their speed and amplitude between those of wild-type slow- and fast-twitch fibers. sox6 homozygotes survived to adulthood and exhibited continued misexpression of tnnc1b as well as smaller slow-twitch fibers. They also exhibited a striking curvature of the spine.\ud \ud Conclusions\ud The Sox6 transcription factor is a key regulator of fast-twitch muscle fiber differentiation in the zebrafish, a role similar to that ascribed to its murine ortholog.
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- 2015
11. Transcriptional analysis of abdominal fat in chickens divergently selected on bodyweight at two ages reveals novel mechanisms controlling adiposity: validating visceral adipose tissue as a dynamic endocrine and metabolic organ.
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Resnyk, C. W., Carré, W., Wang, X., Porter, T. E., Simon, J., Le Bihan-Duval, E., Duclos, M. J., Aggrey, S. E., and Cogburn, L. A.
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ABDOMINAL adipose tissue ,OBESITY in animals ,ANIMAL genetics ,CHICKENS ,POULTRY ,ADIPOGENESIS ,TRANSCRIPTION factors - Abstract
Background: Decades of intensive genetic selection in the domestic chicken (Gallus gallus domesticus) have enabled the remarkable rapid growth of today's broiler (meat-type) chickens. However, this enhanced growth rate was accompanied by several unfavorable traits (i.e., increased visceral fatness, leg weakness, and disorders of metabolism and reproduction). The present descriptive analysis of the abdominal fat transcriptome aimed to identify functional genes and biological pathways that likely contribute to an extreme difference in visceral fatness of divergently selected broiler chickens. Methods: We used the Del-Mar 14 K Chicken Integrated Systems microarray to take time-course snapshots of global gene transcription in abdominal fat of juvenile [1-11 weeks of age (wk)] chickens divergently selected on bodyweight at two ages (8 and 36 wk). Further, a RNA sequencing analysis was completed on the same abdominal fat samples taken from high-growth (HG) and low-growth (LG) cockerels at 7 wk, the age with the greatest divergence in body weight (3.2-fold) and visceral fatness (19.6-fold). Results: Time-course microarray analysis revealed 312 differentially expressed genes (FDR ≤ 0.05) as the main effect of genotype (HG versus LG), 718 genes in the interaction of age and genotype, and 2918 genes as the main effect of age. The RNA sequencing analysis identified 2410 differentially expressed genes in abdominal fat of HG versus LG chickens at 7 wk. The HG chickens are fatter and over-express numerous genes that support higher rates of visceral adipogenesis and lipogenesis. In abdominal fat of LG chickens, we found higher expression of many genes involved in hemostasis, energy catabolism and endocrine signaling, which likely contribute to their leaner phenotype and slower growth. Many transcription factors and their direct target genes identified in HG and LG chickens could be involved in their divergence in adiposity and growth rate. Conclusions: The present analyses of the visceral fat transcriptome in chickens divergently selected for a large difference in growth rate and abdominal fatness clearly demonstrate that abdominal fat is a very dynamic metabolic and endocrine organ in the chicken. The HG chickens overexpress many transcription factors and their direct target genes, which should enhance in situ lipogenesis and ultimately adiposity. Our observation of enhanced expression of hemostasis and endocrine-signaling genes in diminished abdominal fat of LG cockerels provides insight into genetic mechanisms involved in divergence of abdominal fatness and somatic growth in avian and perhaps mammalian species, including humans. [ABSTRACT FROM AUTHOR]
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- 2017
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12. The administration of intranasal live attenuated influenza vaccine induces changes in the nasal microbiota and nasal epithelium gene expression profiles.
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Tarabichi, Y., Li, K., Hu, S., Nguyen, C., Wang, X., Elashoff, D., Saira, K., Frank, Bryan, Bihan, Monika, Ghedin, E., Methé, Barbara A., and Deng, Jane C.
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- 2015
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13. Low hematocrit causes worsened cerebral injury after prolonged hypothermic circulatory arrest in rats: possible involvement of C-Fos, Bcl-2 and Bax
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Wang, X
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Poster Presentation - Published
- 2006
14. The shot-time protective effect of hypoxic preconditioning on ischemia/reperfusion brain
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Wang, X
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Poster Presentation - Published
- 2006
15. Clinical application of an integrated multiparameter system in perioperative body stress assessment
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Wang, X
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Poster Presentation - Published
- 2006
16. Antiapoptotic action of delta opioid peptide [D-Ala2, D-Leu5]enkephalin against oxygen-glucose deprivation in brain slices
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Wang, X
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Poster Presentation - Published
- 2006
17. Neuroprotective effects and mechanisms of fentanyl preconditioning against brain ischemia
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Wang, X
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Poster Presentation - Published
- 2006
18. Role of moesin in hyaluronan induced cell migration in glioblastoma multiforme.
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DeSouza, Leroi V., Matta, Ajay, Karim, Zia, Mukherjee, Joydeep, Simon Wang, X., Krakovska, Olga, Zadeh, Gelareh, Guha, Abhijit, and Michael Siu, K.W.
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MOESIN ,HYALURONIC acid ,CELL migration ,GLIOBLASTOMA multiforme ,PLANT parenchyma - Abstract
Background: A major barrier to effective treatment of glioblastoma multiforme (GBM) is the invasion of glioma cells into the brain parenchyma rendering local therapies such as surgery and radiation therapy ineffective. GBM patients with such highly invasive and infiltrative tumors have poor prognosis with a median survival time of only about a year. However, the mechanisms leading to increased cell migration, invasion and diffused behavior of glioma cells are still poorly understood. Methods: In the current study, we applied quantitative proteomics for the identification of differentially expressed proteins in GBMs as compared to non-malignant brain tissues. Results: Our study led to the identification of 23 proteins showing overexpression in GBM; these include membrane proteins, moesin and CD44. The results were verified using Western blotting and immunohistochemistry in independent set of GBM and non-malignant brain tissues. Both GBM tissues and glioma cell lines (U87 / U373) demonstrated membranous expression of moesin and CD44, as revealed by immunohistochemistry and immunofluorescence, respectively. Notably, glioma cells transfected with moesin siRNA displayed reduced migration and invasion on treatment with hyaluronan (HA), an important component of the extracellular matrix in GBM. CD44, a transmembrane glycoprotein, acts as a major receptor for hyaluronan (HA). Using co-immunoprecipitation assays, we further demonstrated that moesin interacts with CD44 in glioma cells only after treatment with HA; this implicates a novel role of moesin in HA-CD44 signaling in gliomas. Conclusions: Our results suggest that development of inhibitors which interfere with CD44-moesin interactions may open a new avenue in the future to mitigate cellular migration in gliomas. [ABSTRACT FROM AUTHOR]
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- 2013
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19. Grp94-specific monoclonal antibody to counteract BRAF inhibitor resistance in BRAFV600E melanoma
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Sabbatino, F, Favoino, E, Wang, Y, Wang, X, Villani, V, Cai, L, Yang, L, Ferrone, S, and Ferrone, CR
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- 2015
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20. Grp94-specific monoclonal antibody to counteract BRAF inhibitor resistance in BRAFV600E melanoma.
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Sabbatino, F., Favoino, E., Wang, Y., Wang, X., Villani, V., Cai, L., Yang, L., Ferrone, S., and Ferrone, C. R.
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IMMUNOGLOBULINS ,MELANOMA ,HEAT shock proteins - Abstract
The development of BRAF-I resistance in BRAF
V600E melanoma underlies the need to develop strategies to counteract this resistance. It has been shown that administration of heat shock protein 90 (HSP90) inhibitors can counteract multiple mechanisms which drive BRAF-I resistance by reactivation of MAPK and activation of PI3K/AKT pathway. However the clinical application of this strategy is hampered the high toxicity associated with administration of currently available HSP90 inhibitors. To overcome this limitation we have developed a novel monoclonal antibody (mAb), named W9, which recognizes an extracellular epitope of glucose regulated protein of 94 kDa (Grp94), a member of the HSP90 family. The mAb W9 defined Grp94-epitope is selectively expressed on malignant cells but is not detectable on normal cells. Therefore targeting Grp94-epitope by mAb W9 is expected to cause limited if any side effects. The mAb W9 was found to increase and restore the sensitivity to BRAF-I of BRAFV600E melanoma cells including cells which have acquired BRAF-I resistance because of PDGFRa upregulation. [ABSTRACT FROM AUTHOR]- Published
- 2015
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21. Systemic administration of a broadly-neutralizing IgG antibody to generate HIV-neutralization responses in breast milk.
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Fouda, G. G., Amos, J., Beck, K., Smith, S., Wang, X., Reimann, K., and Permar, S.
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BREAST milk - Abstract
An abstract of the research paper "Systemic administration of a broadly-neutralizing IgG antibody to generate HIV-neutralization responses in breast milk," by S. Smith and colleagues is presented.
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- 2012
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22. V5 region in the HIV-1 envelope glycoprotein determines viral sensitivity to the broadly neutralizing monoclonal antibody VRC01.
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Guo, D., Shi, X., Arledge, K., Song, D., Jiang, L., Fu, L., Zhang, S., Wang, X., and Zhang, L.
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HIV ,GLYCOPROTEINS - Abstract
An abstract of the conference paper "V5 Region in the HIV-1 Envelope Glycoprotein Determines Viral Sensitivity to the Broadly Neutralizing Monoclonal Antibody VRC01," by D. Guo and colleagues is presented.
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- 2012
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23. P04.04. Identifying the evidence gaps in acupuncture, experiences of an international project: good practice in traditional Chinese medicine (GP-TCM)
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Robinson, N., Lorenc, A., Ding, W., Jia, J., Bovey, M., and Wang, X.
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- 2012
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24. P01.04. Attenuation of neurological dysfunction and brain infarction with a chinese herbal formula in ischemia-reperfusion induced brain injury of mice.
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Cheng, F., Wang, X., Zhong, X., Zhao, Y., Lu, Y., and Wang, Q.
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BRAIN physiology ,CEREBRAL ischemia ,HERBAL medicine ,STROKE prevention ,REACTIVE oxygen species ,ANIMAL experimentation ,ANTIOXIDANTS ,APOPTOSIS ,BRAIN injuries ,CHINESE medicine ,MICE ,NEURONS ,REPERFUSION ,WESTERN immunoblotting ,DRUG administration ,DRUG dosage ,PREVENTION - Abstract
An abstract of the article "Attenuation of neurological dysfunction and brain infarction with a chinese herbal formula in ischemia-reperfusion induced brain injury of mice," by F. Cheng, X. Wang, and X. Zhong is presented.
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- 2012
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25. Meningitis in patients with a Gram-negative direct cerebrospinal fluid examination: the value of cytochemical markers for the differential diagnosis.
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Zhang H, Wang X, Yang Y, Wu J, Zhang, Hongliang, Wang, Xiaoke, Yang, Yi, and Wu, Jiang
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- 2011
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26. Detection of HPV DNA in esophageal cancer specimens from different regions and ethnic groups: a descriptive study.
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Wang X, Tian X, Liu F, Zhao Y, Sun M, Chen D, Lu C, Wang Z, Shi X, Zhang Q, Zhang D, Shen Z, Li F, Harris CC, Cai H, Ke Y, Wang, Xueqian, Tian, Xiuyun, Liu, Fangfang, and Zhao, Yiqiang
- Abstract
Background: HPV has been found repeatedly in esophageal carcinoma tissues. However, reported detection rates of HPV DNA in these tumors have varied markedly. Differences in detection methods, sample types, and geographic regions of sample origin have been suggested as potential causes of this discrepancy.Methods: HPV L1 DNA and HPV genotypes were evaluated in 435 esophageal carcinoma specimens collected from four geographic regions with different ethnicities including Anyang in north China, Shantou in south China, Xinjiang in west China, and the United States. The HPV L1 fragment was detected using SPF1/GP6+ primers. HPV genotyping was performed using genotype specific PCR.Results: Two hundred and forty four of 435 samples (56.1%) tested positive for HPV L1. Significant differences in detection rate were observed neither among the three areas of China nor between China and the US. HPV6, 16, 18, 26, 45, 56, 57, and 58 were identified in L1 positive samples. HPV16 and 57 were the most common types in all regions, followed by HPV26 and HPV18.Conclusions: HPV infection is common in esophageal carcinoma independent of region and ethnic group of origin. Findings in this study raise the possibility that HPV is involved in esophageal carcinogenesis. Further investigation with a larger sample size over broader geographic areas may be warranted. [ABSTRACT FROM AUTHOR]- Published
- 2010
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27. Variation of cataract surgery costs in four different graded providers of China.
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Fang J, Wang X, Lin Z, Yan J, Yang Y, Li J, Fang, Jiahua, Wang, Xinghua, Lin, Zhende, Yan, Jihua, Yang, Ye, and Li, Jingbo
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Background: China has the largest population of cataract patients in the world. However, the cataract surgery rate per million remains low in China. We carried out a survey on costs of cataract surgery from four different graded providers in China and analyzed differences in cost among these clinics.Methods: 1,189 patients were recruited for the study in four eye clinics, located in two provinces, Guangdong province in southern China and Hubei province in central China. The average cost of each cataract surgery episode was calculated including cost of intraocular lens, cost of drugs and facility cost. We also collected information on reimbursement and disposable annual income of local residents.Results: Mean total cost per cataract intervention of four different providers varied considerably, ranging from US$ 1,293 in Union Hospital to US$ 536 in Jingshan County Hospital. In all providers, except for Jingshan County Hospital, the cost exceeded annual disposable income of local rural residents. As to the proportion of patients with reimbursement, the figure for Union Hospital was only 36%, while for other three clinics it was more than 60%. There was a significant difference between mean reimbursement ratios, with the highest ratio in Zhongshan Ophthalmic Center being 71%.Conclusions: Significant differences in costs of cataract surgery were found among the 4 different graded providers. A part of the cost was borne by patients. Proportion of patients with reimbursement and mean reimbursement ratios were higher in economically developed regions than in economically developing regions. Much more financial support should be directed into the rural New Cooperative Medical Scheme to raise the reimbursement ratio in rural China. [ABSTRACT FROM AUTHOR]- Published
- 2010
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28. Silencing CD36 gene expression results in the inhibition of latent-TGF-beta1 activation and suppression of silica-induced lung fibrosis in the rat.
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Wang X, Chen Y, Lv L, Chen J, Wang, Xin, Chen, Ying, Lv, Lina, and Chen, Jie
- Abstract
Background: The biologically active form of transforming growth factor-beta1 (TGF-beta1) plays a key role in the development of lung fibrosis. CD36 is involved in the transformation of latent TGF-beta1 (L-TGF-beta1) to active TGF-beta1. To clarify the role of CD36 in the development of silica-induced lung fibrosis, a rat silicosis model was used to observe both the inhibition of L-TGF-beta1 activation and the antifibrotic effect obtained by lentiviral vector silencing of CD36 expression.Methods: The rat silicosis model was induced by intratracheal injection of 10 mg silica per rat and CD36 expression was silenced by administration of a lentiviral vector (Lv-shCD36). The inhibition of L-TGF-beta1 activation was examined using a CCL-64 mink lung epithelial growth inhibition assay, while determination of hydroxyproline content along with pathological and immunohistochemical examinations were used for observation of the inhibition of silica-induced lung fibrosis.Results: The lentiviral vector (Lv-shCD36) silenced expression of CD36 in alveolar macrophages (AMs) obtained from bronchoalveolar lavage fluid (BALF) and the activation of L-TGF-beta1 in the BALF was inhibited by Lv-shCD36. The hydroxyproline content of silica+Lv-shCD36 treated groups was significantly lower than in other experimental groups. The degree of fibrosis in the silica+Lv-shCD36-treated groups was less than observed in other experimental groups. The expression of collagen I and III in the silica+Lv-shCD36-treated group was significantly lower than in the other experimental groups.Conclusion: These results indicate that silencing expression of CD36 can result in the inhibition of L-TGF-beta1 activation in a rat silicosis model, thus further preventing the development of silica-induced lung fibrosis. [ABSTRACT FROM AUTHOR]- Published
- 2009
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29. Study on the evaluation of the clinical effects of traditional chinese medicine in heart failure by complex intervention: protocol of SECETCM-HF.
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Mao J, Hou Y, Shang H, Wang H, Wang X, Zhao Y, Niu T, Cui J, Li G, Lin Q, Shi L, Jia X, Fan R, Wang B, Ruan J, Mao, Jingyuan, Hou, Yazhu, Shang, Hongcai, Wang, Henghe, and Wang, Xianliang
- Abstract
Background: Experts in Traditional Chinese Medicine (TCM) have studied the TCM subject of the pathogenesis of heart failure (HF) for several decades. As a result, the general idea is ben deficiency and biao excess. However, the clinical evaluation system which combined the TCM and western medicine in HF has not been developed yet. The objective is to establish the evaluation index system for the integration of TCM and western medicine. The evaluation indexes which include TCM items will specify the research design and methods.Methods: Nine medical centers in different cities in China will participate in the trial. A population of 340 patients with HF will be enrolled through a central randomized system for different test groups. Group A will be treated with only western medicine, while group B with western and Chinese medicine together. The study will last for 12 months from the date of enrollment. The cardiovascular death will be the primary outcome.Discussion: By putting the protocol into practice, the clinical effects of TCM for HF will be identified scientifically, objectively as well as rationally. The proper index system which built in the study will be helpful for the clinical effect expression of HF by integrated medicine in future.Trial Registration: ChiCTR-TRC-00000059. [ABSTRACT FROM AUTHOR]- Published
- 2009
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30. In vivo effects of Pain Relieving Plaster on closed soft tissue injury in rabbit ears.
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Wang Y, Guo C, Zhong H, Zhang W, Wang D, Wang X, and Dong F
- Abstract
Background. Soft tissue injury imposes major public health burdens worldwide. The positive effect of China's Tibetan medicine and the Lamiophlomis rotata-based herbal Pain Relieving Plaster (PRP) on healing closed soft tissue injury (CSTI) has been reported. The herbs contained in Plaster are also referred as 'blood-activating and stasis-dispelling' in herbal medicine. The formula of the plaster contains four China's Tibetan medical herbs, including Lamiophlomis rotata, Oxytropis falcate Bunge, Curcuma longa Linn, and Myricaria bracteata. Two of these herbs (Lamiophlomis rotate; Curcuma longa Linn) are commonly used in different formulae of Chinese medicine. The objective of this study is to use an interdisciplinary approach to test the hypothesis that the formula and its components influence the process of CSTI.Methods. In vivo models have been established in 30 rabbit ear pinnae and studied for: (1) blood flow velocity (BFV) which was affected by pressure of 21.2 kg/cm2 for 30 second over the local rabbit ear tissue; (2) edema formation of the closed soft tissue injury; (3) in vivo local temperature change.Results. The results of in vivo studies indicated that CSTI significantly increased the velocity of blood flow and increased edema formation within the control group. The PRP extracts for 5 hours significantly slowed down the BFV of CSTI in rabbit ears, markedly decreased the elevated edema level from the 3rd to the 5th day.Conclusion. The ingredients contained in the formula have positive effects in healing CSTI and further study is worth exploring. [ABSTRACT FROM AUTHOR]
- Published
- 2008
31. EGFR and HER2 expression in primary cervical cancers and corresponding lymph node metastases: implications for targeted radiotherapy.
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Shen L, Shui Y, Wang X, Sheng L, Yang Z, Xue D, Wei Q, Shen, Li, Shui, Yongjie, Wang, Xiaojia, Sheng, Liming, Yang, Zhengyan, Xue, Danfeng, and Wei, Qichun
- Abstract
Background: Proteins overexpressed on the surface of tumor cells can be selectively targeted. Epidermal growth factor receptor (EGFR) and human epidermal growth factor receptor 2 (HER2) are among the most often targeted proteins. The level and stability of expression in both primary tumors and corresponding metastases is crucial in the assessment of a receptor as target for imaging in nuclear medicine and for various forms of therapy. So far, the expression of EGFR and HER2 has only been determined in primary cervical cancers, and we have not found published data regarding the receptor status in corresponding metastatic lesions. The goal of this study was to evaluate whether any of these receptors are suitable as target for clinical diagnosis and therapy.Methods: Expression of EGFR and HER2 was investigated immunohistochemically in both lymph node metastases and corresponding primary cervical cancers (n = 53). HER2 and EGFR expression was scored using HercepTest criteria (0, 1+, 2+ or 3+).Results: EGFR overexpression (2+ or 3+) was found in 64% (35/53) of the primary cervical tumors and 60% (32/53) of the corresponding lymph node metastases. There was a good concordance between the primary tumors and the paired metastases regarding EGFR expression. Only four patients who had 2+ or 3+ in the primary tumors changed to 0 or 1+ in lymph node metastases, and another two cases changed the other way around. None of the primary tumors or the lymph node metastases expressed HER2 protein.Conclusion: The EGFR expression seems to be common and stable during cervical cancer metastasis, which is encouraging for testing of EGFR targeted radiotherapy. HER2 appears to be of poor interest as a potential target in the treatment of cervical cancer. [ABSTRACT FROM AUTHOR]- Published
- 2008
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32. The development of scoliosis following pinealectomy in two species of chicken with different growth rates.
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Beuerlein, M., Bagnall, Keith, Wang, X., Wilson, J., Raso, James, and Moreau, M.
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SCOLIOSIS - Abstract
An abstract of the article "The development of scoliosis following pinealectomy in two species of chicken with different growth rates" is presented.
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- 2007
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33. Molecular evidence supports simultaneous association of the achlorophyllous orchid Chamaegastrodia inverta with ectomycorrhizal Ceratobasidiaceae and Russulaceae
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Lorenzo Pecoraro, Vijai Kumar Gupta, Giuseppe Venturella, Yusufjon Gafforov, Wenyuan Gao, Xiao Wang, Tingchi Wen, Pecoraro L, Wang X, Venturella G, Gao W, Wen T, Gafforov Y, and Gupta VK
- Subjects
0106 biological sciences ,Microbiology (medical) ,China ,Achlorophyllous orchids ,lcsh:QR1-502 ,Hyphae ,Ceratobasidiaceae ,Plant-fungus interactions ,01 natural sciences ,Microbiology ,Plant Roots ,lcsh:Microbiology ,03 medical and health sciences ,Orchid mycorrhiza ,Symbiosis ,Ascomycota ,Mycology ,Mycorrhizae ,Botany ,Ectomycorrhizal fungi ,Russula ,DNA, Fungal ,Orchidaceae ,Mycelium ,Phylogeny ,030304 developmental biology ,0303 health sciences ,biology ,Settore BIO/02 - Botanica Sistematica ,Basidiomycota ,Endangered Species ,Chaetomium ,biology.organism_classification ,Mycoheterotrophy ,Seedlings ,Achlorophyllous orchids, Ceratobasidiaceae, Ectomycorrhizal fungi, Endangered species, Orchid mycorrhiza, Plant-fungus interactions, Mycoheterotrophy, Russula ,Settore BIO/03 - Botanica Ambientale E Applicata ,Russulaceae ,010606 plant biology & botany ,Research Article - Abstract
Background Achlorophyllous orchids are mycoheterotrophic plants, which lack photosynthetic ability and associate with fungi to acquire carbon from different environmental sources. In tropical latitudes, achlorophyllous forest orchids show a preference to establish mycorrhizal relationships with saprotrophic fungi. However, a few of them have been recently found to associate with ectomycorrhizal fungi and there is still much to be learned about the identity of fungi associated with tropical orchids. The present study focused on mycorrhizal diversity in the achlorophyllous orchid C. inverta, an endangered species, which is endemic to southern China. The aim of this work was to identify the main mycorrhizal partners of C. inverta in different plant life stages, by means of morphological and molecular methods. Results Microscopy showed that the roots of analysed C. inverta samples were extensively colonized by fungal hyphae forming pelotons in root cortical cells. Fungal ITS regions were amplified by polymerase chain reaction, from DNA extracted from fungal mycelia isolated from orchid root samples, as well as from total root DNA. Molecular sequencing and phylogenetic analyses showed that the investigated orchid primarily associated with ectomycorrhizal fungi belonging to a narrow clade within the family Ceratobasidiaceae, which was previously detected in a few fully mycoheterotrophic orchids and was also found to show ectomycorrhizal capability on trees and shrubs. Russulaceae fungal symbionts, showing high similarity with members of the ectomycorrhizal genus Russula, were also identified from the roots of C. inverta, at young seedling stage. Ascomycetous fungi including Chaetomium, Diaporthe, Leptodontidium, and Phomopsis genera, and zygomycetes in the genus Mortierella were obtained from orchid root isolated strains with unclear functional role. Conclusions This study represents the first assessment of root fungal diversity in the rare, cryptic and narrowly distributed Chinese orchid C. inverta. Our results provide new insights on the spectrum of orchid-fungus symbiosis suggesting an unprecedented mixed association between the studied achlorophyllous forest orchid and ectomycorrhizal fungi belonging to Ceratobasidiaceae and Russulaceae. Ceratobasidioid fungi as dominant associates in the roots of C. inverta represent a new record of the rare association between the identified fungal group and fully mycoheterotrophic orchids in nature.
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- 2020
34. Study of hadronic event shape in flavour tagged events e+e- annihilation at <s**(1/2)>=197 GeV
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Achard, P, Adriani, O, Aguilar Benitez, M, Alcaraz, J, Alemanni, G, Allaby, J, Aloisio, A, Alviggi, MG, Anderhub, H, Andreev, VP, Anselmo, F, Arefiev, A, Azemmon, T, Aziz, T, Bagnaia, P, Bajo, A, Baksay, G, Baksay, L, Baldew, SV, Banerjee, S, Banerjee, Sw, Barczyk, A, Barillère, R, Bartalini, P, Basile, M, Batalova, N, Battiston, R, Bay, A, Becker, U, Behner, F, Bellucci, L, Berbeco, R, Berdugo, J, Beges, P, Bertucci, B, Betev, BL, Biasini, M, Bigliettii, M, Biland, A, Blaising, JJ, Blyth, SC, Bobbink, GJ, Böhm, A, Boldizsar, L, Borgia, B, Bottai, S, Bourilkov, D, Bourquin, M, Braccini, S., Branson, J. G., Brochu, F., Burger, JD, Burger, WJ, Cai, XD, Capell, M, Cara Romeo, G, Carlino, G, Cartacci, A, Casaus, J, Cavallari, F, Cavallo, N, Cecchi, C, Cerrada, M, Chamizo, M, Chang, YH, Chemarin, M, Chen, HS, Chiefari, G, Cifarelli, L, Cindolo, F, Clare, I, Clare, R, Coignet, G, Colino, N, Costantini, S, Cruz, B, Cucciarelli, S, Asmundis, R, Déglon, P, Debreczeni, J, Degré, A, Dehmelt, K, Deiters, K, Della Volpe, D, Delmeire, E, Denes, P, De Notaristefani, F, De Salvo, A, Diemoz, M, Dierckxsens, M, Dionisi, C, Dittmar, M, Doria, A, Dova, MT, Duchesneau, D, Duda, M, Echenard, B, Eline, A, El Hage, A, El Mamouni, H, Engler, A, Eppling, FJ, Extermann, P, Falagan, MA, Falciano, S, Favara, A, Fay, J, Fedin, O, Felcini, M, Ferguson, T, Fesefeldt, H, Fiandrini, E, Field, JH, Filthaut, F, Fisher, PH, Fisher, W, Forconi, G, Freudenreich, K, Furetta, C, Galaktionov, Y, Ganguli, SN, Garcia Abia, P, Gataullin, M, Gentile, S, Giagu, S, Gong, ZF, Grenier, G, Grimm, O, Gruenewald, MW, Gupta, VK, Gurtu, A, Gutay, LJ, Haas, D, Hatzifotiadou, D, Hebbeker, T, Hervé, A, Hirschfelder, J, Hofer, H, Hohlmann, M, Holzner, G, Hou, SR, Jin, BN, Jindal, P, Jones, LW, De Jong, P, Josa Mutuberria, I, Kaur, M, Kienzle Focacci, MN, Kim, JK, Kirkby, J, Kittel, W, Klimentov, A, König, AC, Kopal, M, Koutsenko, V, Kräber, M, Kraemer, RW, Krüger, A, Kunin, A, Ladron de Guevara, P, Laktineh, I, Landi, G, Lebeau, M, Lebedev, A, Lebrun, P, Lecomte, P, Lecoq, P, Le Coultre, P, Le Goff, JM, Leiste, R, Levtchenko, M, Levtchenko, P, Li, C, Likhoded, S, Lin, CH, Lin, WT, Linde, FL, Lista, L, Liu, ZA, Lohmann, W, Longo, E, Lu, YS, Luci, C, Luminari, L, Lustermann, W, Ma, WG, Malgeri, L, Malinin, A, Maña, C, Mans, J, Martin, JP, Marzano, F, Mazumdar, K, McNeil, RR, Mele, S, Merola, L, Meschini, M, Metzger, WJ, Mihul, A, Milcent, H, Mirabelli, G, Mnich, J, Mohanty, GB, Muanza, GS, Muijs, JM, Musy, M, Nagy, S, Natale, S, Napolitano, M, Nessi Tedaldi, F, Newman, H, Nisati, A, Novak, T, Nowak, H, Ofierzynski, R, Organtini, G, Pal, I, Palomares, C, Paolucci, P, Paramatti, R, Passeleva, G, Patricielli, S, Paul, T, Pauluzzi, M, Paus, C, Pauss, F, Pedace, M, Perret Gallix, D, Piccolo, D, Pierella, F, Pieri, M, Pioppi, M, Piroué, PA, Pistolesi, E, Plyaskin, V, Pohl, M, Pojidaev, V, Pothier, J, Prokofiev, D, Rahel Callot, G, Rahaman, M. A, Raics, P, Raja, N, Ramelli, R, Rancoita, PG, Ranieri, R, Raspereza, A, Razis, P, Rembeczki, S, Ren, D, Rescigno, M, Reucroft, S, Riemann, S, Riles, K, Roe, BP, Romero, L, Rosca, A, Rosemann, C, Rosenbleck, C, Rosier Lees, S, Roth, S, Rubio, JA, Ruggiero, G, Rykaczewksi, H, Sakharov, A, Saremi, S, Sarkar, S, Salicio, J, Sanchez, E, Schäfer, C, Schegelsky, V, Schopper, H, Schotanus, DJ, Sciacca, C, Servoli, L, Shevchenko, S, Shivarov, N, Shoutko, V, Shumilov, E, Shvorob, A, Son, D, Souga, C, Spillantini, P, Steuer, M, Stickland, DP, Stoyanov, B, Straessner, A, Sudhakar, K, Sultanov, G, Sun, LZ, Sushkov, S, Suter, H, Swain, JD, Szillasi, Z, Tang, XW, Tarjan, P, Tauscher, L, Taylor, L, Tellili, B, Teyssier, D, Timmermans, C, Ting, SCC, Ting, SM, Tonwar, SC, Tóth, J, Tully, C, Tung, KL, Ulbricht, J, Valente, E, Van de Walle, RT, Vasquez, R, Vesztergombi, G, Vetlitsky, I, Viertel, G, Vivargent, M, Vlachos, S, Vodopianov, I, Vogel, H, Vogt, H, Vorobiev, I, Vorobyov, AA, Wadhwa, M, Wang, Q, Wang, XL, Wang, ZM, Weber, M, Wynhoff, S, Xia, L, Xu, ZZ, Yamamoto, JY, Zang, BZ, Yang, CG, Yang, HJ, Jang, M, Yeh, SC, Zalite, A, Zalite, Y, Zhang, ZP, Zhao, J, Zhu, GY, Zhu, RY, Zhuang, HL, Zichichi, A, Zimmmermann, B, Zöller, M., PENSOTTI, SIMONETTA, Achard, P, Adriani, O, Aguilar Benitez, M, Alcaraz, J, Alemanni, G, Allaby, J, Aloisio, A, Alviggi, M, Anderhub, H, Andreev, V, Anselmo, F, Arefiev, A, Azemmon, T, Aziz, T, Bagnaia, P, Bajo, A, Baksay, G, Baksay, L, Baldew, S, Banerjee, S, Barczyk, A, Barillère, R, Bartalini, P, Basile, M, Batalova, N, Battiston, R, Bay, A, Becker, U, Behner, F, Bellucci, L, Berbeco, R, Berdugo, J, Beges, P, Bertucci, B, Betev, B, Biasini, M, Bigliettii, M, Biland, A, Blaising, J, Blyth, S, Bobbink, G, Böhm, A, Boldizsar, L, Borgia, B, Bottai, S, Bourilkov, D, Bourquin, M, Braccini, S, Branson, J, Brochu, F, Burger, J, Burger, W, Cai, X, Capell, M, Cara Romeo, G, Carlino, G, Cartacci, A, Casaus, J, Cavallari, F, Cavallo, N, Cecchi, C, Cerrada, M, Chamizo, M, Chang, Y, Chemarin, M, Chen, H, Chiefari, G, Cifarelli, L, Cindolo, F, Clare, I, Clare, R, Coignet, G, Colino, N, Costantini, S, Cruz, B, Cucciarelli, S, Asmundis, R, Déglon, P, Debreczeni, J, Degré, A, Dehmelt, K, Deiters, K, Della Volpe, D, Delmeire, E, Denes, P, De Notaristefani, F, De Salvo, A, Diemoz, M, Dierckxsens, M, Dionisi, C, Dittmar, M, Doria, A, Dova, M, Duchesneau, D, Duda, M, Echenard, B, Eline, A, El Hage, A, El Mamouni, H, Engler, A, Eppling, F, Extermann, P, Falagan, M, Falciano, S, Favara, A, Fay, J, Fedin, O, Felcini, M, Ferguson, T, Fesefeldt, H, Fiandrini, E, Field, J, Filthaut, F, Fisher, P, Fisher, W, Forconi, G, Freudenreich, K, Furetta, C, Galaktionov, Y, Ganguli, S, Garcia Abia, P, Gataullin, M, Gentile, S, Giagu, S, Gong, Z, Grenier, G, Grimm, O, Gruenewald, M, Gupta, V, Gurtu, A, Gutay, L, Haas, D, Hatzifotiadou, D, Hebbeker, T, Hervé, A, Hirschfelder, J, Hofer, H, Hohlmann, M, Holzner, G, Hou, S, Jin, B, Jindal, P, Jones, L, De Jong, P, Josa Mutuberria, I, Kaur, M, Kienzle Focacci, M, Kim, J, Kirkby, J, Kittel, W, Klimentov, A, König, A, Kopal, M, Koutsenko, V, Kräber, M, Kraemer, R, Krüger, A, Kunin, A, Ladron de Guevara, P, Laktineh, I, Landi, G, Lebeau, M, Lebedev, A, Lebrun, P, Lecomte, P, Lecoq, P, Le Coultre, P, Le Goff, J, Leiste, R, Levtchenko, M, Levtchenko, P, Li, C, Likhoded, S, Lin, C, Lin, W, Linde, F, Lista, L, Liu, Z, Lohmann, W, Longo, E, Lu, Y, Luci, C, Luminari, L, Lustermann, W, Ma, W, Malgeri, L, Malinin, A, Maña, C, Mans, J, Martin, J, Marzano, F, Mazumdar, K, Mcneil, R, Mele, S, Merola, L, Meschini, M, Metzger, W, Mihul, A, Milcent, H, Mirabelli, G, Mnich, J, Mohanty, G, Muanza, G, Muijs, J, Musy, M, Nagy, S, Natale, S, Napolitano, M, Nessi Tedaldi, F, Newman, H, Nisati, A, Novak, T, Nowak, H, Ofierzynski, R, Organtini, G, Pal, I, Palomares, C, Paolucci, P, Paramatti, R, Passeleva, G, Patricielli, S, Paul, T, Pauluzzi, M, Paus, C, Pauss, F, Pedace, M, Pensotti, S, Perret Gallix, D, Piccolo, D, Pierella, F, Pieri, M, Pioppi, M, Piroué, P, Pistolesi, E, Plyaskin, V, Pohl, M, Pojidaev, V, Pothier, J, Prokofiev, D, Rahel Callot, G, Rahaman, M, Raics, P, Raja, N, Ramelli, R, Rancoita, P, Ranieri, R, Raspereza, A, Razis, P, Rembeczki, S, Ren, D, Rescigno, M, Reucroft, S, Riemann, S, Riles, K, Roe, B, Romero, L, Rosca, A, Rosemann, C, Rosenbleck, C, Rosier Lees, S, Roth, S, Rubio, J, Ruggiero, G, Rykaczewksi, H, Sakharov, A, Saremi, S, Sarkar, S, Salicio, J, Sanchez, E, Schäfer, C, Schegelsky, V, Schopper, H, Schotanus, D, Sciacca, C, Servoli, L, Shevchenko, S, Shivarov, N, Shoutko, V, Shumilov, E, Shvorob, A, Son, D, Souga, C, Spillantini, P, Steuer, M, Stickland, D, Stoyanov, B, Straessner, A, Sudhakar, K, Sultanov, G, Sun, L, Sushkov, S, Suter, H, Swain, J, Szillasi, Z, Tang, X, Tarjan, P, Tauscher, L, Taylor, L, Tellili, B, Teyssier, D, Timmermans, C, Ting, S, Tonwar, S, Tóth, J, Tully, C, Tung, K, Ulbricht, J, Valente, E, Van de Walle, R, Vasquez, R, Vesztergombi, G, Vetlitsky, I, Viertel, G, Vivargent, M, Vlachos, S, Vodopianov, I, Vogel, H, Vogt, H, Vorobiev, I, Vorobyov, A, Wadhwa, M, Wang, Q, Wang, X, Wang, Z, Weber, M, Wynhoff, S, Xia, L, Xu, Z, Yamamoto, J, Zang, B, Yang, C, Yang, H, Jang, M, Yeh, S, Zalite, A, Zalite, Y, Zhang, Z, Zhao, J, Zhu, G, Zhu, R, Zhuang, H, Zichichi, A, Zimmmermann, B, and Zöller, M
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L3,CERN-LEP-L3 ,FIS/04 - FISICA NUCLEARE E SUBNUCLEARE - Published
- 2008
35. Exploring the link between MORF4L1 and risk of breast cancer
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Martrat, G., Maxwell, C.A., Tominaga, E., Porta-de-la-Riva, M., Bonifaci, N., Gomez-Baldo, L., Bogliolo, M., Lazaro, C., Blanco, I., Brunet, J., Aguilar, H., Fernandez-Rodriguez, J., Seal, S., Renwick, A., Rahman, N., Kuhl, J., Neveling, K., Schindler, D., Ramirez, M.J., Castella, M., Hernandez, G., Easton, D.F., Peock, S., Cook, M., Oliver, C.T., Frost, D., Platte, R., Evans, D.G., Lalloo, F., Eeles, R., Izatt, L., Chu, C., Davidson, R., Ong, K.R., Cook, J., Douglas, F., Hodgson, S., Brewer, C., Morrison, P.J., Porteous, M., Peterlongo, P., Manoukian, S., Peissel, B., Zaffaroni, D., Roversi, G., Barile, M., Viel, A., Pasini, B., Ottini, L., Putignano, A.L., Savarese, A., Bernard, L., Radice, P., Healey, S., Spurdle, A., Chen, X.Q., Beesley, J., Rookus, M.A., Verhoef, S., Tilanus-Linthorst, M.A., Vreeswijk, M.P., Asperen, C.J., Bodmer, D., Ausems, M.G.E.M., Os, T.A. van, Blok, M.J., Meijers-Heijboer, H.E.J., Hogervorst, F.B.L., Goldgar, D.E., Buys, S., John, E.M., Miron, A., Southey, M., Daly, M.B., Harbst, K., Borg, A., Rantala, J., Barbany-Bustinza, G., Ehrencrona, H., Stenmark-Askmalm, M., Kaufman, B., Laitman, Y., Milgrom, R., Friedman, E., Domchek, S.M., Nathanson, K.L., Rebbeck, T.R., Oskar, T., Couch, F.J., Wang, X.S., Fredericksen, Z., Cuadras, D., Moreno, V., Pientka, F.K., Depping, R., Caldes, T., Osorio, A., Benitez, J., Bueren, J., Heikkinen, T., Nevanlinna, H., Hamann, U., Torres, D., Caligo, M.A., Godwin, A.K., Imyanitov, E.N., Janavicius, R., Sinilnikova, O.M., Stoppa-Lyonnet, D., Mazoyer, S., Verny-Pierre, C., Castera, L., Pauw, A. de, Bignon, Y.J., Uhrhammer, N., Peyrat, J.P., Vennin, P., Ferrer, S.F., Collonge-Rame, M.A., Mortemousque, I., McGuffog, L., Chenevix-Trench, G., Pereira-Smith, O.M., Antoniou, A.C., Ceron, J., Tominaga, K., Surralles, J., Pujana, M.A., EMBRACE, kConFab, HEBON, BCFR, SWE-BRCA, GEMO Study Collaborators, Human Genetics, BMC, Ed., Translational Research Laboratory, Catalan Institute of Oncology-Bellvitge Institute for Biomedical Research, Biomedical Research Centre Network for Epidemiology and Public Health (CIBERESP), Catalan Institute of Oncology, Department of Cellular and Structural Biology, The University of Texas Health Science Center at Houston (UTHealth)-Sam and Ann Barshop Institute for Longevity and Aging Studies, Chemoresistance and Predictive Factors of Tumor Response and Stromal Microenvironment, Institut d'Investigació Biomèdica de Bellvitge [Barcelone] (IDIBELL), Biomarkers and Susceptibility Unit, Department of Genetics and Microbiology, Universitat Autònoma de Barcelona (UAB), Biomedical Research Centre Network for Rare Diseases (CIBERER), Genetic Counseling and Hereditary Cancer Programme, Section of Cancer Genetics, Institute of cancer research, Department of Human Genetics, Julius-Maximilians-Universität Würzburg (JMU), Strangeways Research Laboratory, University of Cambridge [UK] (CAM)-Department of Public Health and Primary Care-Centre for Cancer Genetic Epidemiology, Centre for Cancer Genetic Epidemiology [Cambridge], University of Cambridge [UK] (CAM)-Department of Oncology, Genetic Medicine, St Mary's Hospital-NHS Foundation Trust-Manchester Academic Health Sciences Centre-Central Manchester University Hospitals, Oncogenetics Team, The Institute of Cancer Research and Royal Marsden NHS Foundation Trust, Clinical Genetics Department, Guy's and St Thomas NHS Foundation Trust, Yorkshire Regional Genetics Service, St James's hospital, Ferguson-Smith Centre for Clinical Genetics, West Midlands Regional Genetics Service, Birmingham Women's and Children's NHS Foundation Trust, Sheffield Clinical Genetics Service, Sheffield Children's NHS Foundation Trust, Institute of Human Genetics, Newcastle Upon Tyne Hospitals NHS Foundation Trust, Queen Mary University of London (QMUL)-St George's Hospital, Department of Clinical Genetics, Royal Devon & Exeter Hospital, Northern Ireland Regional Genetics Centre, Belfast City Hospital, South East of Scotland Regional Genetics Service, Western General Hospital, Unit of Molecular Bases of Genetic Risk and Genetic Testing, Department of Preventive and Predictive Medicine-Fondazione IRCCS Istituto Nazionale Tumori (INT), Department of Preventive and Predictive Medicine, IFOM, Istituto FIRC di Oncologia Molecolare (IFOM), Unit of Medical Genetics, Fondazione IRCCS INT, Division of Cancer Prevention and Genetics, Istituto Europeo di Oncologia (IEO), Division of Experimental Oncology 1, Centro di Riferimento Oncologico (CRO), Department of Genetics, Biology and Biochemistry, Università degli studi di Torino = University of Turin (UNITO), Department of Molecular Medicine, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome] (UNIROMA), Università degli Studi di Firenze = University of Florence (UniFI), Fiorgen Foundation for Pharmacogenomics, Division of Medical Oncology, Regina Elena Cancer Institute, Department of Experimental Oncology, IEO, Division of Genetics and Population Health, Queensland Institute of Medical Research, Department of Epidemiology, The Netherlands Cancer Institute, Family Cancer Clinic, Department of Surgical Oncology, Erasmus University Medical Center [Rotterdam] (Erasmus MC)-Family Cancer Clinic, Center for Human and Clinical Genetics, Leiden University Medical Center (LUMC), DNA Diagnostics, Radboud University Medical Center [Nijmegen], Department of Medical Genetics, University Medical Center [Utrecht], Academic Medical Center - Academisch Medisch Centrum [Amsterdam] (AMC), University of Amsterdam [Amsterdam] (UvA)-University of Amsterdam [Amsterdam] (UvA), University Hospital Maastricht, VU Medical Center, Department of Dermatology, University of Utah School of Medicine [Salt Lake City], Department of Internal Medicine, Huntsman Cancer Institute, Cancer Prevention Institute of California, Department of Cancer Biology, Dana-Farber Cancer Institute [Boston], Department of Surgery, Harvard Medical School [Boston] (HMS), Centre for Molecular, Environmental, Genetic and Analytic Epidemiology (MEGA), University of Melbourne-Melbourne School of Population Health, Division of Population Science, Fox Chase Cancer Center, Department of Oncology, Clinical Sciences, Lund University [Lund]-Skåne University Hospital, Karolinska University Hospital [Stockholm], Department of Genetics and Pathology, Uppsala University, Department of Oncology, University Hospital-Hälsouniversitetet Universitetssjukhuset, The Institute of Oncology, Chaim Sheba Medical Center, The Susanne Levy Gertner Oncogenetics Unit, Sackler Faculty of Medicine, Tel Aviv University (TAU), Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania-University of Pennsylvania, Department of Medicine, Medical Genetics, Abramson Cancer Center-Perelman School of Medicine, Center for Clinical Epidemiology and Biostatistics, Faculty of Medicine, University of Iceland [Reykjavik], Department of Laboratory Medicine and Pathology, Mayo Clinic, Department of Health Sciences Research, Statistical Assessment Service, Department of Physiology, Universität zu Lübeck = University of Lübeck [Lübeck]-Center for Structural and Cell Biology in Medicine, Medical Oncology Branch, Hospital Clínico San Carlos, Human Cancer Genetics Programme, CIBER de Enfermedades Raras (CIBERER)-Spanish National Cancer Research Centre, Division of Hematopoiesis and Gene Therapy, Centro de Investigaciones Energéticas Medioambientales y Tecnológicas [Madrid] (CIEMAT), Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Molecular Genetics of Breast Cancer, German Cancer Research Center - Deutsches Krebsforschungszentrum [Heidelberg] (DKFZ), Instituto de Genética Humana, Pontificia Universidad Javeriana (PUJ), Section of Genetic Oncology, University of Pisa - Università di Pisa, Department of Pathology and Laboratory Medicine, University of Kansas Medical Center [Kansas City, KS, USA], Laboratory of Molecular Oncology, N.N. Petrov Institute of Oncology, Department of Molecular and Regenerative Medicine, Hematology, Oncology and Transfusion, Vilnius University [Vilnius]-Hospital Santariskiu Clinics, Unité Mixte de Génétique Constitutionnelle des Cancers Fréquents, Centre Léon Bérard [Lyon]-Hospices Civils de Lyon (HCL), Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de Génétique Oncologique, Institut Curie [Paris], Unité de génétique et biologie des cancers (U830), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Département d'Oncogénétique, Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER-UNICANCER, Laboratoire d'Oncologie Moléculaire Humaine, Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] (UNICANCER/Lille), Université de Lille-UNICANCER-Université de Lille-UNICANCER, Consultation d'Oncogénétique, Laboratoire de Génétique Chromosomique, CH Chambéry, Département de Génétique et Reproduction, Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon)-Hôpital Saint-Jacques, Service de génétique [Tours], Centre Hospitalier Régional Universitaire de Tours (CHRU Tours)-Hôpital Bretonneau, The CIMBA data management is supported by Cancer Research - UK., kConFab, HEBON, BCFR, SWE-BRCA, GEMO Study Collaborators, Autonomous University of Barcelona, Julius-Maximilians-Universität Würzburg [Wurtzbourg, Allemagne] (JMU), Department of Oncology-University of Cambridge [UK] (CAM), University of Turin, Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Università degli Studi di Firenze = University of Florence [Firenze] (UNIFI), Departament of Genetics and Pathology, Uppsala University-Rudbeck Laboratory, Tel Aviv University [Tel Aviv], University of Pennsylvania [Philadelphia]-University of Pennsylvania [Philadelphia], Universität zu Lübeck [Lübeck]-Center for Structural and Cell Biology in Medicine, University of Kansas Medical Center [Lawrence], Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5), Université Lille Nord de France (COMUE)-UNICANCER-Université Lille Nord de France (COMUE)-UNICANCER, Universiteit Leiden-Universiteit Leiden, Skåne University Hospital-Lund University [Lund], University of Pennsylvania [Philadelphia]-University of Pennsylvania [Philadelphia]-Abramson Cancer Center, Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Bretonneau-Centre Hospitalier Régional Universitaire de Tours (CHRU Tours), Clinical Genetics, Faculteit der Geneeskunde, Klinische Genetica, RS: GROW - School for Oncology and Reproduction, Genetica & Celbiologie, Easton, Douglas [0000-0003-2444-3247], Antoniou, Antonis [0000-0001-9223-3116], Apollo - University of Cambridge Repository, Martrat, G, Maxwell, C, Tominaga, E, Porta de la Riva, M, Bonifaci, N, Gómez Baldó, L, Bogliolo, M, Lázaro, C, Blanco, I, Brunet, J, Aguilar, H, Fernández Rodríguez, J, Seal, S, Renwick, A, Rahman, N, Kühl, J, Neveling, K, Schindler, D, Ramírez, M, Castellà, M, Hernández, G, Embrace, Easton, D, Peock, S, Cook, M, Oliver, C, Frost, D, Platte, R, Evans, D, Lalloo, F, Eeles, R, Izatt, L, Chu, C, Davidson, R, Ong, K, Cook, J, Douglas, F, Hodgson, S, Brewer, C, Morrison, P, Porteous, M, Peterlongo, P, Manoukian, S, Peissel, B, Zaffaroni, D, Roversi, G, Barile, M, Viel, A, Pasini, B, Ottini, L, Putignano, A, Savarese, A, Bernard, L, Radice, P, Healey, S, Spurdle, A, Chen, X, Beesley, J, Rookus, M, Verhoef, S, Tilanus Linthorst, M, Vreeswijk, M, Asperen, C, Bodmer, D, Ausems, M, van Os, T, Blok, M, Meijers Heijboer, H, Hogervorst, F, Goldgar, D, Buys, S, John, E, Miron, A, Southey, M, Daly, M, Harbst, K, Borg, A, Rantala, J, Barbany Bustinza, G, Ehrencrona, H, Stenmark Askmalm, M, Kaufman, B, Laitman, Y, Milgrom, R, Friedman, E, Domchek, S, Nathanson, K, Rebbeck, T, Johannsson, O, Couch, F, Wang, X, Fredericksen, Z, Cuadras, D, Moreno, V, Pientka, F, Depping, R, Caldés, T, Osorio, A, Benítez, J, Bueren, J, Heikkinen, T, Nevanlinna, H, Hamann, U, Torres, D, Caligo, M, Godwin, A, Imyanitov, E, Janavicius, R, Sinilnikova, O, Stoppa Lyonnet, D, Mazoyer, S, Verny Pierre, C, Castera, L, de Pauw, A, Bignon, Y, Uhrhammer, N, Peyrat, J, Vennin, P, Ferrer, S, Collonge Rame, M, Mortemousque, I, Mcguffog, L, Chenevix Trench, G, Pereira Smith, O, Antoniou, A, Cerón, J, Tominaga, K, Surrallés, J, Pujana, M, Human genetics, CCA - Oncogenesis, Biomedical Research Centre Network for Epidemiology and Public Health ( CIBERESP ), The University of Texas Health Science Center at San Antonio-Sam and Ann Barshop Institute for Longevity and Aging Studies, Institut d'Investigació Biomèdica de Bellvitge [Barcelone] ( IDIBELL ), Biomedical Research Centre Network for Rare Diseases ( CIBERER ), University of Würzburg, University of Cambridge [UK] ( CAM ) -Department of Public Health and Primary Care-Centre for Cancer Genetic Epidemiology, University of Cambridge [UK] ( CAM ) -Department of Oncology, Birmingham Women's Hospital Healthcare NHS Trust, Sheffield Children's Hospital, Newcastle Upon Tyne Hospitals NHS Trust, Queen Mary University of London ( QMUL ) -St George's Hospital, IFOM, Istituto FIRC di Oncologia Molecolare ( IFOM ), Università degli Studi di Roma 'La Sapienza' [Rome], University of Florence, Erasmus MC-Daniel den Hoed Cancer Center-Family Cancer Clinic, University Medical Center Utrecht, Academic Medical Center [Amsterdam] ( AMC ), University of Amsterdam [Amsterdam] ( UvA ) -University of Amsterdam [Amsterdam] ( UvA ), Harvard Medical School [Boston] ( HMS ), Centre for Molecular, Environmental, Genetic and Analytic Epidemiology ( MEGA ), University of Pennsylvania School of Medicine, University of Pennsylvania School of Medicine-Abramson Cancer Center, Centro de Investigaciones Energéticas, Deutsches Krebsforschungszentrum ( DKFZ ), Pontificia Universidad Javeriana, University of Pisa [Pisa], University of Kansas Medical Center, Centre Léon Bérard [Lyon]-Hospices Civils de Lyon ( HCL ), Centre de Recherche en Cancérologie de Lyon ( CRCL ), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), INSTITUT CURIE, Unité de génétique et biologie des cancers ( U830 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut Curie-Institut National de la Santé et de la Recherche Médicale ( INSERM ), CRLCC Jean Perrin, CRLCC Oscar Lambret, Centre Hospitalier Régional Universitaire [Besançon] ( CHRU Besançon ) -Hôpital Saint-Jacques, Hôpital Bretonneau-CHRU Tours, and Universitat de Barcelona
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DNA Repair ,Genes, BRCA2 ,RAD51 ,Genes, BRCA1 ,Germ-Cell ,Helicase Brip1 ,medicine.disease_cause ,[ SDV.CAN ] Life Sciences [q-bio]/Cancer ,Mice ,0302 clinical medicine ,Breast cancer ,Fanconi anemia ,Risk Factors ,Replication Protein A ,Teknik och teknologier ,Homologous Recombination ,skin and connective tissue diseases ,C-Elegans ,Genetics ,Medicine(all) ,ddc:616 ,0303 health sciences ,Mutation ,Fanconi Anemia Complementation Group D2 Protein ,Nuclear Proteins ,Anèmia aplàstica ,3. Good health ,030220 oncology & carcinogenesis ,Chromodomain Protein ,Engineering and Technology ,Female ,RNA Interference ,Fanconi Anemia Complementation Group N Protein ,Aplastic anemia ,Research Article ,BRCA2 Mutation Carrier ,DNA repair ,PALB2 ,Breast Neoplasms ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Biology ,Cell Line ,Càncer de mama ,Genomic disorders and inherited multi-system disorders [IGMD 3] ,03 medical and health sciences ,SDG 3 - Good Health and Well-being ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,Two-Hybrid System Techniques ,medicine ,Genetic predisposition ,Animals ,Humans ,Genetic Predisposition to Disease ,Caenorhabditis elegans ,Gene ,030304 developmental biology ,Phenocopy ,Caenorhabditis-Elegan ,Tumor Suppressor Proteins ,medicine.disease ,BRCA1 ,BRCA2 ,Pancreatic-Cancer ,Fanconi Anemia ,Genes ,Cancer and Oncology ,Fanconi-Anemia ,Cancer research ,Rad51 Recombinase ,Susceptibility Gene ,DNA Damage ,Transcription Factors - Abstract
Introduction Proteins encoded by Fanconi anemia (FA) and/or breast cancer (BrCa) susceptibility genes cooperate in a common DNA damage repair signaling pathway. To gain deeper insight into this pathway and its influence on cancer risk, we searched for novel components through protein physical interaction screens. Methods Protein physical interactions were screened using the yeast two-hybrid system. Co-affinity purifications and endogenous co-immunoprecipitation assays were performed to corroborate interactions. Biochemical and functional assays in human, mouse and Caenorhabditis elegans models were carried out to characterize pathway components. Thirteen FANCD2-monoubiquitinylation-positive FA cell lines excluded for genetic defects in the downstream pathway components and 300 familial BrCa patients negative for BRCA1/2 mutations were analyzed for genetic mutations. Common genetic variants were genotyped in 9,573 BRCA1/2 mutation carriers for associations with BrCa risk. Results A previously identified co-purifying protein with PALB2 was identified, MRG15 (MORF4L1 gene). Results in human, mouse and C. elegans models delineate molecular and functional relationships with BRCA2, PALB2, RAD51 and RPA1 that suggest a role for MRG15 in the repair of DNA double-strand breaks. Mrg15-deficient murine embryonic fibroblasts showed moderate sensitivity to γ-irradiation relative to controls and reduced formation of Rad51 nuclear foci. Examination of mutants of MRG15 and BRCA2 C. elegans orthologs revealed phenocopy by accumulation of RPA-1 (human RPA1) nuclear foci and aberrant chromosomal compactions in meiotic cells. However, no alterations or mutations were identified for MRG15/MORF4L1 in unclassified FA patients and BrCa familial cases. Finally, no significant associations between common MORF4L1 variants and BrCa risk for BRCA1 or BRCA2 mutation carriers were identified: rs7164529, Ptrend = 0.45 and 0.05, P2df = 0.51 and 0.14, respectively; and rs10519219, Ptrend = 0.92 and 0.72, P2df = 0.76 and 0.07, respectively. Conclusions While the present study expands on the role of MRG15 in the control of genomic stability, weak associations cannot be ruled out for potential low-penetrance variants at MORF4L1 and BrCa risk among BRCA2 mutation carriers.
36. Early-life milk replacer feeding mediates lipid metabolism disorders induced by colonic microbiota and bile acid profiles to reduce body weight in goat model.
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Zhang K, Zhang T, Guo M, Cuoji A, Xu Y, Zhao Y, Yang Y, Brugger D, Wang X, Suo L, Wu Y, and Chen Y
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Background: Dysregulation of lipid metabolism and its consequences on growth performance in young ruminants have attracted attention, especially in the context of alternative feeding strategies. This study aims to elucidate the effects of milk replacer (MR) feeding on growth, lipid metabolism, colonic epithelial gene expression, colonic microbiota composition and systemic metabolism in goat kids compared to breast milk (BM) feeding, addressing a critical knowledge gap in early life nutrition., Methods: Ten female goat kids were divided into 2 groups: those fed breast milk (BM group) and those fed a milk replacer (MR group). Over a period of 28 d, body weight was monitored and blood and tissue samples were collected for biochemical, transcriptomic and metabolomic analyses. Profiling of the colonial microbiota was performed using 16S rRNA gene sequencing. Intestinal microbiota transplantation (IMT) experiments in gnotobiotic mice were performed to validate causality., Results: MR-fed pups exhibited reduced daily body-weight gain due to impaired lipid metabolism as evidenced by lower serum and liver total cholesterol (TC) and non-esterified fatty acid (NEFA) concentrations. Transcriptomic analysis of the colonic epithelium revealed upregulated genes involved in negative regulation of lipid metabolism, concomitant with microbiota shifts characterized by a decrease in Firmicutes and an increase in Actinobacteria. Specifically, genera such as Bifidobacterium and Prevotella were enriched in the MR group, while Clostridium and Faecalibacterium were depleted. Metabolomics analyses confirmed alterations in bile acid and fatty acid metabolic pathways. IMT experiments in mice recapitulated the metabolic phenotype observed in MR-fed goats, confirming the role of the microbiota in modulating host lipid metabolism., Conclusions: Milk replacer feeding in goat kids disrupts lipid metabolism and gut microbiota dynamics, resulting in reduced growth rates and metabolic alterations. These findings highlight the importance of early nutritional intervention on metabolic programming and suggest that modulation of the gut microbiota may be a target for improving growth and metabolic health in ruminants. This study contributes to the understanding of nutritional management strategies in livestock and their impact on animal health and productivity., (© 2024. The Author(s).)
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- 2024
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37. Abscopal effect: from a rare phenomenon to a new frontier in cancer therapy.
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Wang X, Zhang H, XinZhang, and Liu Y
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Radiotherapy (RT) controls local lesions, meantime it has the capability to induce systemic response to inhibit distant, metastatic, non-radiated tumors, which is referred to as the "abscopal effect". It is widely recognized that radiotherapy can stimulate systemic immune response. This provides a compelling theoretical basis for the combination of immune therapy combined with radiotherapy(iRT). Indeed, this phenomenon has also been observed in clinical treatment, bringing significant clinical benefits to patients, and a series of basic studies are underway to amplify this effect. However, the molecular mechanisms of immune response induced by RT, determination of the optimal treatment regimen for iRT, and how to amplify the abscopal effect. In order to amplify and utilize this effect in clinical management, these key issues require to be well addressed; In this review, we comprehensively summarize the growing consensus and emphasize the emerging limitations of enhancing the abscopal effect with radiotherapy or immunotherapy. Finally, we discuss the prospects and barriers to the current clinical translational applications., (© 2024. The Author(s).)
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- 2024
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38. Primary yolk sac tumor of the endometrium combined with situs inversus totalis: a case report and literature review.
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Liu R, Wang Y, Wang X, Chen X, and Hu J
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- Humans, Female, Adult, alpha-Fetoproteins analysis, Hysterectomy methods, Endodermal Sinus Tumor complications, Endodermal Sinus Tumor diagnosis, Endodermal Sinus Tumor pathology, Situs Inversus complications, Situs Inversus diagnosis, Endometrial Neoplasms complications, Endometrial Neoplasms diagnosis, Endometrial Neoplasms pathology
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Background: Yolk sac tumor (YST) is a highly malignant germ cell tumor, a majority of which originate from the gonads and are extremely rare from endometrium., Case Presentation: Here we present a case of a 42-year-old woman suffered from primary pure yolk sac tumor of the endometrium complicated with situs inversus totalis. The patient presented at our hospital with irregular vaginal bleeding. Imageological examination showed a space-occupying lesion in the cervix and the serum Alpha-fetoprotein (AFP) level was significantly high (more than 1210ng/ml). Then she underwent total hysterectomy, bilateral salpingo-oophorectomy and pelvic lymph node dissection. The subsequent postoperative pathological diagnosis was yolk sac tumor arising from the endometrium. Next, the patient was treated with 6 cycles of chemotherapy with Pingyangmycin, etoposide and cisplatin regimen and was alive without evidence of recurrence or distant metastases for 13 months., Conclusions: This rare disease needs to be differentiated from endometrial epithelial neoplasia and the significant increase in AFP is helpful for diagnosis. Combined with previous literature reports, comprehensive staging laparotomy or maximum cytoreductive surgery complemented by standard chemotherapy can usually achieve a good efficacy., (© 2024. The Author(s).)
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- 2024
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39. Tumor microenvironment-responsive manganese-based nano-modulator activate the cGAS-STING pathway to enhance innate immune system response.
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Liang X, Wang D, Zhao Y, Wang X, Yao S, Huang W, Yang Y, Dong X, Zhang L, and Yang J
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- Animals, Mice, Manganese chemistry, Manganese pharmacology, Humans, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, Cell Line, Tumor, Reactive Oxygen Species metabolism, Neoplasms drug therapy, Neoplasms immunology, Female, Mice, Inbred C57BL, Nucleotidyltransferases metabolism, Tumor Microenvironment drug effects, Immunity, Innate drug effects, Manganese Compounds chemistry, Manganese Compounds pharmacology, Membrane Proteins metabolism, Signal Transduction drug effects, Oxides chemistry, Oxides pharmacology
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Background: Manganese ions (Mn
2+ ) combined with adjuvants capable of damaging and lysing tumor cells form an antitumor nano-modulator that enhances the immune efficacy of cancer therapy through the cascade activation of the cyclic GMP-AMP interferon gene synthase-stimulator (cGAS-STING) pathway, which underscores the importance of developing antitumor nano-modulators, which induce DNA damage and augment cGAS-STING activity, as a critical future research direction. METHODS AND RESULTS: We have successfully synthesized an antitumor nano-modulator, which exhibits good dispersibility and biosafety. This nano-modulator is engineered by loading manganese dioxide nanosheets (M-NS) with zebularine (Zeb), known for its immunogenicity-enhancing effects, and conducting targeted surface modification using hyaluronic acid (HA). After systemic circulation to the tumor site, Mn2+ , Zeb, and reactive oxygen species (ROS) are catalytically released in the tumor microenvironment by H+ and H2 O2. These components can directly or indirectly damage the DNA or mitochondria of tumor cells, thereby inducing programmed cell death. Furthermore, they promote the accumulation of double-stranded DNA (dsDNA) in the cytoplasm, enhancing the activation of the cGAS-STING signalling pathway and boosting the production of type I interferon and the secretion of pro-inflammatory cytokines. Additionally, Zeb@MH-NS enhances the maturation of dendritic cells, the infiltration of cytotoxic T lymphocytes, and the recruitment of natural killer cells at the tumor site., Conclusions: This HA-modified manganese-based hybrid nano-regulator can enhance antitumor therapy by boosting innate immune activity and may provide new directions for immunotherapy and clinical translation in cancer., (© 2024. The Author(s).)- Published
- 2024
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40. Serum BAFF level is associated with the presence and severity of coronary artery disease and acute myocardial infarction.
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Chen Z, Wang Z, Cui Y, Xie H, Yi L, Zhu Z, Ni J, Du R, Wang X, Zhu J, Ding F, Quan W, Zhang R, Wang Y, and Yan X
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- Humans, Male, Female, Middle Aged, Aged, Case-Control Studies, Risk Factors, Risk Assessment, Prognosis, B-Cell Activating Factor blood, Coronary Artery Disease blood, Coronary Artery Disease diagnostic imaging, Coronary Artery Disease diagnosis, Biomarkers blood, Severity of Illness Index, Myocardial Infarction blood, Myocardial Infarction diagnosis, Coronary Angiography, Predictive Value of Tests, Up-Regulation
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Objective: The aim of this study was to investigate the relationship between circulating levels of B cell activating factor (BAFF) and the presence and severity of coronary artery disease (CAD) and acute myocardial infarction (AMI) in humans, as its biological functions in this context remain unclear., Methods: Serum BAFF levels were measured in a cohort of 723 patients undergoing angiography, including 204 patients without CAD (control group), 220 patients with stable CAD (CAD group), and 299 patients with AMI (AMI group). Logistic regression analyses were used to assess the association between BAFF and CAD or AMI., Results: Significantly elevated levels of BAFF were observed in patients with CAD and AMI compared to the control group. Furthermore, BAFF levels exhibited a positive correlation with the SYNTAX score (r = 0.3002, P < 0.0001) and the GRACE score (r = 0.5684, P < 0.0001). Logistic regression analysis demonstrated that increased BAFF levels were an independent risk factor for CAD (adjusted OR 1.305, 95% CI 1.078-1.580) and AMI (adjusted OR 2.874, 95% CI 1.708-4.838) after adjusting for confounding variables. Additionally, elevated BAFF levels were significantly associated with a high GRACE score (GRACE score 155 to 319, adjusted OR 4.297, 95% CI 1.841-10.030). BAFF exhibited a sensitivity of 75.0% and specificity of 71.4% in differentiating CAD patients with a high SYNTAX score, and a sensitivity of 75.5% and specificity of 72.8% in identifying AMI patients with a high GRACE score., Conclusion: Circulating BAFF levels serve as a valuable diagnostic marker for CAD and AMI. Elevated BAFF levels are associated with the presence and severity of these conditions, suggesting its potential as a clinically relevant biomarker in cardiovascular disease., (© 2024. The Author(s).)
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- 2024
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41. Study on the association between malnutrition, early childhood caries and caries activity among children aged 3-5 years.
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Wang D, Wang X, Zhao C, Ma S, Zhang Y, and Shi H
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- Humans, Child, Preschool, Male, Female, China epidemiology, Malnutrition complications, Malnutrition epidemiology, Thinness complications, Overweight complications, Nutritional Status, Hemoglobins analysis, Risk Factors, Body Mass Index, Obesity complications, Obesity epidemiology, Anthropometry, Dental Caries epidemiology, DMF Index
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Background: This study aims to investigate the association between malnutrition and early childhood caries (ECC) and caries activity among children aged 3-5 years, in order to provide a theoretical basis for preventing and blocking ECC and improving malnutrition., Methods: Children aged 3-5 years from six kindergartens in Zhao Xian, China were enrolled in this study. The decayed, missing, filled teeth (dmft) of all children were examined and recorded. The Cariostat method was used to detect dental caries activity, collect anthropometric data and measure haemoglobin concentration. Parents were asked to complete a questionnaire on the general characteristics and oral health behaviour of the participants. The "Growth Standards for Chinese Children Under 7 Years Old" was used to assess the nutritional status of all participating children. Wilcoxon rank sum test and multivariate logistic regression analysis were used to analyse and evaluate the relationship between ECC, caries activity and malnutrition., Results: A total of 635 children who met the criteria were included in this study. After adjusting for confounding factors, logistic regression showed that the risk of ECC was significantly increased in underweight children compared with normal children (OR = 5.43, P < 0. 05); compared with normal children, the risk of ECC decreased in overweight and obese children (OR = 0.31, P < 0.001); underweight children had higher caries severity than normal weight children, and the difference was statistically significant (OR = 2.69, P < 0. 05); stunted children had higher caries severity than normal weight children and the difference was statistically significant (OR = 2.28, P < 0.05); underweight was positively associated with caries activity and the association was statistically significant (OR = 2.33, P < 0. 05); stunting was positively associated with caries activity and the association was statistically significant (OR = 2.1, P < 0.05); overweight and obesity were negatively associated with caries activity and the association was statistically significant (OR = 0.61, P < 0.05)., Conclusions: The risk of ECC among children aged 3-5 years was positively associated with undernutrition and negatively associated with overnutrition. The severity of ECC among children aged 3-5 years was positively associated with undernutrition. The caries activity among children aged 3-5 years was positively associated with undernutrition and negatively associated with overnutrition., (© 2024. The Author(s).)
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- 2024
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42. Associations between non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio and hyperuricemia: a cross-sectional study.
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Jiang Z, Zhu X, Zhao D, Jiang H, Wang X, and Su F
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- Humans, Male, Female, Cross-Sectional Studies, Middle Aged, Adult, Prevalence, Risk Factors, Logistic Models, Aged, Cholesterol blood, Cholesterol, LDL blood, Hyperuricemia blood, Hyperuricemia epidemiology, Cholesterol, HDL blood, Nutrition Surveys
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Background and Objective: The value of the non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio (NHHR) assessment in the context of metabolic abnormalities is growing in importance. Nevertheless, the relationship between NHHR and hyperuricemia (HUA) is unknown. This study seeks to investigate the relationship between NHHR and HUA., Methods: The data derived from the 2017-2020 National Health and Nutrition Examination Survey (NHANES) included 7,876 adult participants. The multivariable logistic regression model, subgroup analysis and smooth fitting curve were utilized in order to investigate the association between NHHR and HUA., Results: In the fully adjusted model 3, NHHR was significantly associated with HUA. Specifically, participants in the highest quartile of NHHR had 1.95 times higher odds of HUA prevalence compared to those in the lowest quartile [2.95 (2.39, 3.64), P < 0.0001]. Although the overall trend suggested a positive association, further analysis using smooth fitting curves and threshold effect analysis indicated that this association was nonlinear, with an inflection point at 5.8. The positive association persisted across different HUA definitions and after removing outliers. Subgroup analysis showed significant interactions between NHHR and HUA in different races and diabetes statuses. The odds of HUA prevalence were higher among non-diabetic participants [1.40 (1.32, 1.49), P < 0.0001] compared to diabetic participants [1.18 (1.06, 1.32), P = 0.0031]. Mexican Americans had the lowest odds of HUA prevalence [1.09 (0.92, 1.27), P = 0.2413] compared to other races., Conclusions: There is a significant positive association between NHHR and HUA, indicating that NHHR may serve as a potential risk assessment maker for HUA, although further prospective studies are needed for validation., (© 2024. The Author(s).)
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- 2024
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43. MRI-based assessment paraspinal extensor muscle fatty infiltration in acute cervical spinal cord injury patients - a retrospective study.
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Liao Y, Lin X, Su W, Wu X, Wang X, Yang W, Lu H, Huang C, and Wu Y
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- Humans, Male, Female, Retrospective Studies, Middle Aged, Aged, Adult, Case-Control Studies, Spinal Cord Injuries diagnostic imaging, Spinal Cord Injuries pathology, Paraspinal Muscles diagnostic imaging, Paraspinal Muscles pathology, Magnetic Resonance Imaging, Cervical Vertebrae diagnostic imaging, Cervical Vertebrae pathology, Adipose Tissue diagnostic imaging, Adipose Tissue pathology
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Background: The effect of fat infiltration in the paraspinal muscles on cervical degenerative disease has been confirmed by multiple studies. However, little is known about fat infiltration in the paraspinal extensors in patients with acute cervical spinal cord injury (SCI). This study aimed to investigate the difference in paraspinal extensor fatty infiltration between patients with acute cervical SCI and healthy controls, and to further explore the protective role of the paravertebral extensor muscles in patients with cervical SCI., Methods: A total of 50 patients with acute cervical SCI admitted to the emergency department from January 2019 to November 2023 were retrospectively analyzed, including 26 males and 24 females, with an average age of 59.60 ± 10.81 years. A control group of 50 healthy middle-aged and elderly individuals was also included, comprising 28 males and 22 females, with an average age of 55.00 ± 8.21 years. Cervical spine magnetic resonance imaging (MRI) was used to measure the cross-sectional areas of the superficial and deep cervical extensor muscles, the corresponding vertebral body cross-sectional areas, and the fat area within the superficial and deep extensor muscle groups using Image J software. Differences between the two groups were compared, and the cervical SCI patients were further analyzed based on the severity of the spinal cord injury and gender differences., Results: The deep fatty infiltration ratio (DFIR) and superficial fatty infiltration ratio (SFIR) at C4-C7 in the cervical SCI group were significantly higher than those in the control group (P < 0.001). The cross-sectional area of the functional deep extensor area (FDEA) relative to the vertebral body area (VBA) and the cross-sectional area of the functional superficial extensor area (FSEA) relative to the VBA at the C5 and C6 levels in the cervical SCI group were significantly lower than those in the control group (P < 0.001, P < 0.001, P = 0.034, P = 0.004 respectively). Among the cervical SCI patients, the cross-sectional areas of the deep extensor area (DEA) and the superficial extensor area (SEA) in males were significantly higher than those in females (P < 0.001). At the C6 and C7 levels, the FDEA/VBA and FSEA/VBA ratios in the male group were higher than those in the female group (P = 0.009, P = 0.022, P = 0.019, P = 0.005, respectively)., Conclusion: Patients with acute cervical SCI exhibit significantly higher fatty infiltration and a greater degree of paravertebral extensor muscle degeneration compared to healthy controls. This finding underscores the importance of the paravertebral extensor muscles in the context of cervical SCI and may guide future therapeutic strategies., (© 2024. The Author(s).)
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- 2024
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44. No genetic causal association between human papillomavirus and lung cancer risk: a bidirectional two-sample Mendelian randomization analysis.
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Chen Y, Xu Z, Zhang Z, Wang X, and Dong M
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- Humans, Risk Factors, Risk Assessment, Carcinoma, Squamous Cell virology, Carcinoma, Squamous Cell genetics, Carcinoma, Squamous Cell epidemiology, Papillomavirus E7 Proteins genetics, Genetic Predisposition to Disease, Adenocarcinoma genetics, Adenocarcinoma virology, Adenocarcinoma epidemiology, Human papillomavirus 18 genetics, Adenocarcinoma of Lung genetics, Adenocarcinoma of Lung virology, Polymorphism, Single Nucleotide, Phenotype, Human Papillomavirus Viruses, Mendelian Randomization Analysis, Lung Neoplasms genetics, Lung Neoplasms virology, Lung Neoplasms epidemiology, Papillomavirus Infections virology, Papillomavirus Infections genetics, Genome-Wide Association Study
- Abstract
Introduction: Several observational or retrospective studies have previously been conducted to explore the possible association between lung cancer and human papillomavirus (HPV) infection. However, there may be inconsistencies in the data and conclusions due to differences in study design and HPV testing methods. There are currently no studies that provide conclusive evidence to support the involvement of HPV in the occurrence and development of lung cancer. Therefore, the relationship between HPV and lung cancer remains controversial and uncertain. This study aimed to explore whether HPV infection is causally related to lung cancer risk by systematically performing a two-way Two-Sample Mendelian Randomization (TSMR) analysis., Methods: In the International Lung Cancer Consortium (ILCCO) genome-wide association study dataset, we included 11,348 lung cancer (LUCA) cases, including 3275 squamous cell carcinoma (LUSC) cases, 3442 adenocarcinoma (LUAD) cases, and 15,861 cases of control. Using genetic variants associated with the HPV E7 protein as instrumental variables, we summarized statistics associated with HPV infection in the MRC IEU OpenGWAS database, which included the HPV-16 E7 protein and the HPV-18 E7 protein. Two-sample Mendelian randomization (MR) results are expressed as odds ratios (OR) and 95% confidence intervals (CI)., Results: Based on a comprehensive analysis of genome-wide association study (GWAS) data from public databases, we mainly used inverse-variance weighted (IVW) to estimate causal relationships, while using MR-Egger, weighted median, simple mode, and weighted mode, and other four methods as supplements. Two-sample MR Analysis revealed no causal relationship between exposure factors (HPV-16 E7 protein and HPV-18 E7 protein) and outcome factors (lung cancer (LUCA) and its subtypes squamous cell carcinoma (LUSC) and adenocarcinoma (LUAD)) in forward MR Analysis using the IVW approach.HPV-16 E7 protein and LUCA and its subtypes LUSC and LUAD by IVW method results: [OR] = 1.002; 95% [CI]: 0.961 - 1.045; p = 0.920; [OR] = 1.023; 95% [CI]: 0.966 - 1.084; p = 0.438; [OR] = 0.994; 95% [CI]: 0.927 - 1.066; p = 0.872); HPV-18 E7 protein and LUCA and its subtypes LUSC and LUAD by IVW method results: [OR] = 0.965; 95% [CI]: 0.914 - 1.019; p = 0.197; [OR] = 0.933; 95% [CI]: 0.834 - 1.043; p = 0.222; [OR] = 1.028; 95% [CI]: 0.945 - 1.118; p = 0.524. It was observed through reverse MR that LUCA and its subtypes LUSC and LUAD were used as exposure factors, and HPV infection (HPV-16 E7 protein and HPV-18 E7 protein) was used as the outcome factors, the results of the IVW method are also invalid.LUCA and HPV-16 E7 protein and HPV-18 E7 protein by IVW method results: [OR] = 1.036; 95% [CI]: 0.761 - 1.411; p = 0.82; [OR] = 1.318; 95% [CI]: 0.949 - 1.830; p = 0.099; LUSC and HPV-16 E7 protein and HPV-18 E7 protein by IVW method results: [OR] = 1.123; 95% [CI]0.847 - 1.489; p = 0.421; [OR] = 0.931; 95% [CI]: 0.660 - 1.313; p = 0.682; LUAD and HPV-16 E7 protein and HPV-18 E7 protein by IVW method results: [OR] = 1.182; 95% [CI] 0.983 - 1.421; p = 0.075; [OR] = 1.017; 95% [CI]: 0.817 - 1.267; p = 0.877.Our results indicate that there is no causal relationship between genetically predicted HPV infection and LUCA and its subtypes LUSC and LUAD. In addition, in the reverse MR analysis, we did not observe a significant causal relationship between LUCA and its subtypes LUSC and LUAD on HPV infection., Conclusions: Our findings do not support a genetic association between HPV infection and lung cancer., (© 2024. The Author(s).)
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- 2024
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45. COVID-19 in the Tibet, China, the roof of the world: a comparative analysis of high-altitude residents and newcomers.
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Yan X, Shan Y, Luo Q, Liu D, Zhang Y, Zhai Q, Zhou Z, Wang W, La B, Yan J, Zhu X, Wang X, Thuen L, Chen W, Li Q, Zeng J, Tian G, Chen X, Ci Q, Zhou Q, Jin X, and Pingcuo T
- Subjects
- Humans, Female, Male, Tibet epidemiology, Adult, Middle Aged, Cohort Studies, Aged, Young Adult, Hemoglobins analysis, Adolescent, COVID-19 epidemiology, Altitude, SARS-CoV-2
- Abstract
Background: After a 920-day hiatus, COVID-19 resurged in the Tibet Autonomous Region of China in August 2022. This study compares the characteristics of COVID-19 between high-altitude residents and newcomers, as well as between newcomers and lowlanders., Methods: This multi-center cohort study conducted at the Third People's Hospital of Tibet Autonomous Region and Beijing University Shenzhen Hospital, included 520 high-altitude resident patients, 53 high-altitude newcomer patients, and 265 lowlander patients infected with the Omicron variant. Initially, we documented epidemiological, clinical, and treatment data across varying residency at admission. We compared the severity of COVID-19 and various laboratory indicators, including hemoglobin concentration and SpO2%, over a 14-day period from the date of the first positive nucleic acid test, as well as the differences in treatment methods and disease outcomes between highlanders and high-altitude newcomers. We also compared several characteristics of COVID-19 between high-altitude newcomers and lowlanders. Univariate analysis, multivariable logistic regression, and the generalized linear mixed model were utilized for the analysis., Results: No fatalities were observed. The study found no significant differences in COVID-19 severity or in the physiological measures of hemoglobin concentration and SpO
2 % between high-altitude and lowland residents. Similarly, there were no statistically significant differences in the values or trends of hemoglobin and SpO2 % between high-altitude residents and newcomers throughout the 14-day observation period. However, compared to age- and sex-matched lowlander patients (1:5 ratio), high-altitude newcomers exhibited higher heart rates, respiratory rates, and average hemoglobin concentrations, along with lower platelet counts. There were no significant differences in hospital stays between the two groups., Conclusions: High-altitude residents and newcomer patients exhibit clinical similarities. However, the clinical characteristics of high-altitude newcomers and lowlander patients differ due to the impact of the high-altitude environment. These results highlight potential considerations for public health strategies in high-altitude regions such as Tibet., (© 2024. The Author(s).)- Published
- 2024
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46. Single-cell and spatial sequencing identifies senescent and germinal tumor cells in adamantinomatous craniopharyngiomas.
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Wang X, Lin J, Liu H, Zhao C, Tu Z, Xu D, Zhang E, Zhou Z, Qi X, Wang X, and Lin Z
- Abstract
Adamantinomatous craniopharyngioma (ACP) is a clinically aggressive tumor without effective treatment method. Previous studies proposed a paracrine tumorigenesis model, in which oncogenic β-catenin induces senescence in pituitary stem cells and the senescent cells lead the formation of paracrine tumors through secretion of pro-tumorigenic factors. However, there lacks characterization on senescent cells in ACPs. Here, we profiled 12 ACPs with single-cell RNA and TCR-sequencing to elucidate the cellular atlas in ACPs and 3 of them were also subject to spatial sequencing to localize different subpopulations of the tumor cells. In total, we obtained the transcriptome profiles of 70,682 cells. Tumor cells, which were unambiguously identified through the cellular mutation status of the driver CTNNB1 mutations, were clustered into 6 subsets. The whorl-like cluster (WC) cells show distinct molecular features from the other tumor cells and the palisading epithelium (PE) cells consists of a proliferating subset. Other than typical PE and WC, we identified two novel subpopulations of the tumor cells. In one subpopulation, the cells express a high level of cytokines, e.g., FDCSP and S100A8/A9, and are enriched with the senescence-associated secretory phenotype (SASP) factors. Hematoxylin and eosin staining reveals that these SASP cells lack an ordered structures and their nuclei are elongated. In the other subpopulation, the cell sizes are small and they are tightly packed together with an unusual high density expressing a high level of mitochondrial genes (median 10.9%). These cells are the origin of the tumor developmental trajectories revealed by RNA velocity and pseudo-time analysis. Single-cell RNA and TCR analysis reveals that some ACPs are infiltrated with clonally expanded cytotoxic T cells. We propose a hypothesis that WC and PE are formed via different negative regulation mechanisms of the overactivated WNT/β-catenin signaling which provides a new understanding on the tumorigenesis of ACPs. The study lays a foundation for future studies on targeting senescent cells in ACPs with senolytic compounds or other therapeutic agents., (© 2024. The Author(s).)
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- 2024
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47. 23-year review of spheno-orbital meningioma: clinical, radiological, and pathological insights from 100 cases.
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Liu Y, Ma M, Li X, Hei Y, Li Y, Ma R, Wang X, Wang Q, Yang X, and Wu W
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- Humans, Male, Middle Aged, Female, Adult, Retrospective Studies, Aged, Neoplasm Recurrence, Local, Follow-Up Studies, Young Adult, Meningioma diagnostic imaging, Meningioma pathology, Meningioma diagnosis, Orbital Neoplasms diagnostic imaging, Orbital Neoplasms pathology, Orbital Neoplasms diagnosis, Meningeal Neoplasms pathology, Meningeal Neoplasms diagnostic imaging, Meningeal Neoplasms diagnosis, Magnetic Resonance Imaging, Sphenoid Bone pathology, Sphenoid Bone diagnostic imaging, Tomography, X-Ray Computed
- Abstract
Background: Spheno-orbital meningioma (SOM) represents a unique variant of sphenoid wing meningiomas, distinguished by its propensity for bone infiltration and cranio-orbital involvement. SOM exhibits a considerable incidence of misdiagnosis and recurrence., Purposes: To elucidate the clinical, radiological, and pathological characteristics of SOM., Methods: Review of electronic medical records, histopathology, radiological images and follow-up information of 100 SOM patients., Results: Of the 100 patients (28 males, 72 females) with SOM, mean age was 46.8 ± 12.6 years and prevalent symptoms were proptosis (99%). All the CT scans showed hyperostosis with 89.3% of the hyperostosis having an irregular edge. In MRI scans, dural tail sign was observed across all patients and the cranio-orbital tumors often penetrated temporal muscle (74.1%), extraocular muscle (74.1%) and lacrimal gland (63%). All the 100 patients underwent surgical intervention, and among them, 62 individuals received postoperative radiotherapy. Grade I resections had a lower recurrence rate(16.7%), which further decreased with the addition of radiotherapy(13.9%). In contrast, all patients with grade II or higher grade resections without radiotherapy experienced recurrence, indicating a higher risk associated with less complete tumor removal. The pathological examination revealed that intraorbital sections exhibited comparable tumor density to intraorbital SOM tumors, along with increased fibrous density but decreased vascular distribution., Conclusions: Radiological characteristics of SOM included cranio-orbital tumors, hyperostosis of the sphenoid wing with an irregular edge, and dural tail sign. Combination of gross total resection and adjuvant radiotherapy was recommended to minimize recurrence rate. Intracranial SOM tumors tended to be softer and more bleed-prone than intraorbital sections, necessitating surgical precision., (© 2024. The Author(s).)
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- 2024
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48. Whole-transcriptome sequencing analysis to identify key circRNAs, miRNAs, and mRNAs in the development of yak testes.
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Hu L, Wang X, Guo S, Cao M, Kang Y, Ding Z, Pei J, Ge Q, Ma Y, and Guo X
- Subjects
- Male, Animals, Cattle genetics, Spermatogenesis genetics, Sequence Analysis, RNA, Transcriptome, Gene Ontology, Gene Regulatory Networks, Testis metabolism, Testis growth & development, RNA, Circular genetics, MicroRNAs genetics, RNA, Messenger genetics, RNA, Messenger metabolism, Gene Expression Profiling
- Abstract
Background: The Testis is an important reproductive organ in male mammals and the site for spermatogenesis, androgen synthesis, and secretion. Non-coding RNAs (ncRNAs) play an important regulatory role in various biological processes. However, the regulatory role of ncRNAs in the development of yak testes and spermatogenesis remains largely unclear., Result: In this study, we compared the expression profiles of circular RNAs (circRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) in yak testicular tissue samples collected at 6 months (Y6M), 18 months (Y18M), and 4 years (Y4Y). Using RNA sequencing (RNA-Seq), we observed a significant difference in the expression patterns of ncRNAs in the samples collected at different testicular development stages. Twenty-two differentially expressed (DE) circRNAs, 69 DE miRNAs, and 64 DE mRNAs were detected in Y6M, Y18M, and Y4Y testicular samples, respectively. The results of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that the source genes of DE circRNAs, predicted target genes of DE miRNAs, and DE mRNAs were specifically associated with signaling pathways and GO terms that were related to sperm synthesis, sperm vitality, and testicular development, such as cell cycle, Wnt signaling pathway, MAPK signaling pathway, GnRH signaling pathway, and spermatogenesis. The analysis of the circRNA-miRNA-mRNA network revealed that some DE ncRNAs, including miR-574, miR-449a, CDC42, and CYP11A1, among others, may be involved in testicular spermatogenesis. Concurrently, various circRNA-miRNA interaction pairs were observed., Conclusion: Our findings provide a database of circRNAs, miRNAs, and mRNAs expression profiles in testicular tissue of yaks at different developmental stages and a detailed understanding of the regulatory network of ncRNAs in yak testicular development and provide data that can help elucidate the molecular mechanisms underlying yak testicular development., (© 2024. The Author(s).)
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- 2024
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49. CT-based radiomics nomogram to predict proliferative hepatocellular carcinoma and explore the tumor microenvironment.
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Wang G, Ding F, Chen K, Liang Z, Han P, Wang L, Cui F, Zhu Q, Cheng Z, Chen X, Huang C, Cheng H, Wang X, and Zhao X
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- Humans, Male, Female, Middle Aged, Cell Proliferation, ROC Curve, Aged, Retrospective Studies, Cohort Studies, Prognosis, Radiomics, Liver Neoplasms diagnostic imaging, Liver Neoplasms pathology, Carcinoma, Hepatocellular diagnostic imaging, Carcinoma, Hepatocellular pathology, Nomograms, Tumor Microenvironment, Tomography, X-Ray Computed
- Abstract
Background: Proliferative hepatocellular carcinomas (HCCs) is a class of aggressive tumors with poor prognosis. We aimed to construct a computed tomography (CT)-based radiomics nomogram to predict proliferative HCC, stratify clinical outcomes and explore the tumor microenvironment., Methods: Patients with pathologically diagnosed HCC following a hepatectomy were retrospectively collected from two medical centers. A CT-based radiomics nomogram incorporating radiomics model and clinicoradiological features to predict proliferative HCC was constructed using the training cohort (n = 184), and validated using an internal test cohort (n = 80) and an external test cohort (n = 89). The predictive performance of the nomogram for clinical outcomes was evaluated for HCC patients who underwent surgery (n = 201) or received transarterial chemoembolization (TACE, n = 104). RNA sequencing data and histological tissue slides from The Cancer Imaging Archive database were used to perform transcriptomics and pathomics analysis., Results: The areas under the receiver operating characteristic curve of the radiomics nomogram to predict proliferative HCC were 0.84, 0.87, and 0.85 in the training, internal test, and external test cohorts, respectively. The radiomics nomogram could stratify early recurrence-free survivals in the surgery outcome cohort (hazard ratio [HR] = 2.25; P < 0.001) and progression-free survivals in the TACE outcome cohort (HR = 2.21; P = 0.03). Transcriptomics and pathomics analysis indicated that the radiomics nomogram was associated with carbon metabolism, immune cells infiltration, TP53 mutation, and heterogeneity of tumor cells., Conclusion: The CT-based radiomics nomogram could predict proliferative HCC, stratify clinical outcomes, and measure a pro-tumor microenvironment., (© 2024. The Author(s).)
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- 2024
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50. Radiogenomic profiling of global DNA methylation associated with molecular phenotypes and immune features in glioma.
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Zhuang Z, Lin J, Wan Z, Weng J, Yuan Z, Xie Y, Liu Z, Xie P, Mao S, Wang Z, Wang X, Huang M, Luo Y, and Yu H
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- Humans, Female, Male, Middle Aged, Adult, Machine Learning, Phenotype, Aged, Biomarkers, Tumor genetics, Glioma genetics, Glioma immunology, DNA Methylation genetics, Magnetic Resonance Imaging, Brain Neoplasms genetics, Brain Neoplasms diagnostic imaging, Brain Neoplasms immunology, Brain Neoplasms pathology
- Abstract
Background: The radiogenomic analysis has provided valuable imaging biomarkers with biological insights for gliomas. The radiogenomic markers for molecular profile such as DNA methylation remain to be uncovered to assist the molecular diagnosis and tumor treatment., Methods: We apply the machine learning approaches to identify the magnetic resonance imaging (MRI) features that are associated with molecular profiles in 146 patients with gliomas, and the fitting models for each molecular feature (MoRad) are developed and validated. To provide radiological annotations for the molecular profiles, we devise two novel approaches called radiomic oncology (RO) and radiomic set enrichment analysis (RSEA)., Results: The generated MoRad models perform well for profiling each molecular feature with radiomic features, including mutational, methylation, transcriptional, and protein profiles. Among them, the MoRad models have a remarkable performance in quantitatively mapping global DNA methylation. With RO and RSEA approaches, we find that global DNA methylation could be reflected by the heterogeneity in volumetric and textural features of enhanced regions in T2-weighted MRI. Finally, we demonstrate the associations of global DNA methylation with clinicopathological, molecular, and immunological features, including histological grade, mutations of IDH and ATRX, MGMT methylation, multiple methylation-high subtypes, tumor-infiltrating lymphocytes, and long-term survival outcomes., Conclusions: Global DNA methylation is highly associated with radiological profiles in glioma. Radiogenomic global methylation is an imaging-based quantitative molecular biomarker that is associated with specific consensus molecular subtypes and immune features., (© 2024. The Author(s).)
- Published
- 2024
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