8 results on '"Tiss A"'
Search Results
2. Fetuin-A levels are increased in the adipose tissue of diabetic obese humans but not in circulation
- Author
-
Khadir, Abdelkrim, Kavalakatt, Sina, Madhu, Dhanya, Hammad, Maha, Devarajan, Sriraman, Tuomilehto, Jaakko, and Tiss, Ali
- Published
- 2018
- Full Text
- View/download PDF
3. Fetuin-a expression profile in mouse and human adipose tissue
- Author
-
Dhanya Madhu, Abdelkrim Khadir, Ali Tiss, and Sina Kavalakatt
- Subjects
Pathology ,medicine.medical_specialty ,Human adipose primary cells ,Clinical chemistry ,alpha-2-HS-Glycoprotein ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Adipokine ,Adipose tissue ,Clinical nutrition ,Intra-Abdominal Fat ,Fetuin-a ,Mice ,Endocrinology ,Subcutaneous Tissue ,medicine ,Mouse adipose tissue ,Animals ,Humans ,Mouse Adipose Tissue ,lcsh:RC620-627 ,Cells, Cultured ,business.industry ,Biochemistry (medical) ,Fetuin ,lcsh:Nutritional diseases. Deficiency diseases ,medicine.anatomical_structure ,Adipose Tissue ,Commentary ,business ,Subcutaneous tissue ,Lipidology - Abstract
Fetuin-A (Fet-A) was one of the first hepatokines to be reportedly linked to metabolic diseases. Fet-A was also suggested to be an adipokine, but its expression in the adipose tissue remains debatable. Here we compared the expression of Fet-A between human and mice adipose tissue biopsies as well as among human subcutaneous tissue and visceral adipose tissue primary cells, and mouse 3 T3-L1 cells at various stages of differentiation. Fet-A was expressed in mice biopsies and cells but not in human biopsies and cells, except in visceral adipose tissue primary cells following differentiation. Although the marginal expression of Fet-A in human visceral adipose tissue, a major contribution of Fet-A expression in human adipose tissue to systemic Fet-A levels is discounted, but it could indicate specific local Fet-A action in the visceral adipose tissue.
- Published
- 2020
4. Biomarker discovery and redundancy reduction towards classification using a multi-factorial MALDI-TOF MS T2DM mouse model dataset
- Author
-
Frank Kleinjung, Rainer Cramer, T. Dreja, Ali Tiss, Alexandra Chadt, Hadi Al-Hasani, Celia Smith, Mark W. Towers, Chris Bauer, Johannes Schuchhardt, Dieter Beule, and Knut Reinert
- Subjects
Male ,Proteomics ,Factorial ,Mice, Obese ,Computational biology ,Biology ,lcsh:Computer applications to medicine. Medical informatics ,Bioinformatics ,Biochemistry ,Reduction (complexity) ,Hemoglobins ,Mice ,Structural Biology ,Albumins ,Redundancy (engineering) ,Animals ,Biomarker discovery ,lcsh:QH301-705.5 ,Molecular Biology ,Analysis of Variance ,Applied Mathematics ,Diet ,Computer Science Applications ,Mice, Inbred C57BL ,Matrix-assisted laser desorption/ionization ,ComputingMethodologies_PATTERNRECOGNITION ,lcsh:Biology (General) ,Diabetes Mellitus, Type 2 ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,lcsh:R858-859.7 ,DNA microarray ,Peptides ,Biomarkers ,Research Article - Abstract
Background Diabetes like many diseases and biological processes is not mono-causal. On the one hand multi-factorial studies with complex experimental design are required for its comprehensive analysis. On the other hand, the data from these studies often include a substantial amount of redundancy such as proteins that are typically represented by a multitude of peptides. Coping simultaneously with both complexities (experimental and technological) makes data analysis a challenge for Bioinformatics. Results We present a comprehensive work-flow tailored for analyzing complex data including data from multi-factorial studies. The developed approach aims at revealing effects caused by a distinct combination of experimental factors, in our case genotype and diet. Applying the developed work-flow to the analysis of an established polygenic mouse model for diet-induced type 2 diabetes, we found peptides with significant fold changes exclusively for the combination of a particular strain and diet. Exploitation of redundancy enables the visualization of peptide correlation and provides a natural way of feature selection for classification and prediction. Classification based on the features selected using our approach performs similar to classifications based on more complex feature selection methods. Conclusions The combination of ANOVA and redundancy exploitation allows for identification of biomarker candidates in multi-dimensional MALDI-TOF MS profiling studies with complex experimental design. With respect to feature selection our method provides a fast and intuitive alternative to global optimization strategies with comparable performance. The method is implemented in R and the scripts are available by contacting the corresponding author.
- Published
- 2011
5. Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue
- Author
-
Kazem Behbehani, Samia Warsame, Naser Elkum, Irina Al-Khairi, Fahad Al-Ghimlas, Said Dermime, Jehad Abubaker, Abdelkrim Khadir, Jeena John, Mohamed Abu-Farha, Preethi Cherian, Ali Tiss, Sina Kavalakatt, and Mohammed Dehbi
- Subjects
medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Adipose tissue ,Physical exercise ,Inflammation ,HSP72 Heat-Shock Proteins ,Biology ,Insulin resistance ,Endocrinology ,Heat shock protein ,Internal medicine ,Diabetes mellitus ,medicine ,HSP ,Humans ,Obesity ,Heat shock ,Exercise ,Heat-Shock Proteins ,Biochemistry, medical ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Research ,Biochemistry (medical) ,medicine.disease ,Immunohistochemistry ,Increased inflammatory response ,medicine.symptom ,ER stress ,Heat-Shock Response - Abstract
Background Obesity is characterized by a chronic low-grade inflammation and altered stress responses in key metabolic tissues. Impairment of heat shock response (HSR) has been already linked to diabetes and insulin resistance as reflected by decrease in heat shock proteins (HSPs) expression. However, the status of HSR in non-diabetic human obese has not yet been elucidated. The aim of the current study was to investigate whether obesity triggers a change in the HSR pattern and the impact of physical exercise on this pattern at protein and mRNA levels. Methods Two groups of adult non-diabetic human subjects consisting of lean and obese (n = 47 for each group) were enrolled in this study. The expression pattern of HSP-27, DNAJB3/HSP-40, HSP-60, HSC-70, HSP72, HSP-90 and GRP-94 in the adipose tissue was primarily investigated by immunohistochemistry and then complemented by western blot and qRT-PCR in Peripheral blood mononuclear cells (PBMCs). HSPs expression levels were correlated with various physical, clinical and biochemical parameters. We have also explored the effect of a 3-month moderate physical exercise on the HSPs expression pattern in obese subjects. Results Obese subjects displayed increased expression of HSP-60, HSC-70, HSP-72, HSP-90 and GRP-94 and lower expression of DNAJB3/HSP-40 (P
- Published
- 2014
6. Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue.
- Author
-
Tiss, Ali, Khadir, Abdelkrim, Abubaker, Jehad, Abu-Farha, Mohamed, Al-Khairi, Irina, Cherian, Preethi, John, Jeena, Kavalakatt, Sina, Warsame, Samia, Al-Ghimlas, Fahad, Elkum, Naser, Behbehani, Kazem, Dermime, Said, and Dehbi, Mohammed
- Subjects
- *
GLUCOSE-regulated proteins , *OBESITY , *HEAT shock proteins , *ADIPOSE tissues , *EXERCISE , *IMMUNOHISTOCHEMISTRY - Abstract
Background Obesity is characterized by a chronic low-grade inflammation and altered stress responses in key metabolic tissues. Impairment of heat shock response (HSR) has been already linked to diabetes and insulin resistance as reflected by decrease in heat shock proteins (HSPs) expression. However, the status of HSR in non-diabetic human obese has not yet been elucidated. The aim of the current study was to investigate whether obesity triggers a change in the HSR pattern and the impact of physical exercise on this pattern at protein and mRNA levels. Methods Two groups of adult non-diabetic human subjects consisting of lean and obese (n = 47 for each group) were enrolled in this study. The expression pattern of HSP-27, DNAJB3/HSP- 40, HSP-60, HSC-70, HSP72, HSP-90 and GRP-94 in the adipose tissue was primarily investigated by immunohistochemistry and then complemented by western blot and qRTPCR in Peripheral blood mononuclear cells (PBMCs). HSPs expression levels were correlated with various physical, clinical and biochemical parameters. We have also explored the effect of a 3-month moderate physical exercise on the HSPs expression pattern in obese subjects. Results Obese subjects displayed increased expression of HSP-60, HSC-70, HSP-72, HSP-90 and GRP-94 and lower expression of DNAJB3/HSP-40 (P < 0.05). No differential expression was observed for HSP-27 between the two groups. Higher levels of HSP-72 and GRP-94 proteins correlated positively with the indices of obesity (body mass index and percent body fat) and circulating levels of IFN-gamma-inducible protein 10 (IP-10) and RANTES chemokines. This expression pattern was concomitant with increased inflammatory response in the adipose tissue as monitored by increased levels of Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α), and RANTES (P < 0.05). Physical exercise reduced the expression of various HSPs in obese to normal levels observed in lean subjects with a parallel decrease in the endogenous levels of IL-6, TNF-α, and RANTES. Conclusion Taken together, these data indicate that obesity triggers differential regulation of various components of the HSR in non-diabetic subjects and a 3-month physical moderate exercise was sufficient to restore the normal expression of HSPs in the adipose tissue with concomitant attenuation in the inflammatory response. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
7. Biomarker Discovery and Redundancy Reduction towards Classification using a Multi-factorial MALDI-TOF MS T2DM Mouse Model Dataset.
- Author
-
Bauer, Chris, Kleinjung, Frank, Smith, Celia J., Towers, Mark W., Tiss, Ali, Chadt, Alexandra, Dreja, Tanja, Beule, Dieter, Al-Hasani, Hadi, Reinert, Knut, Schuchhardt, Johannes, and Cramer, Rainer
- Subjects
BIOMARKERS ,DIABETES ,PROTEINS ,BIOINFORMATICS ,PEPTIDES - Abstract
Background: Diabetes like many diseases and biological processes is not mono-causal. On the one hand multifactorial studies with complex experimental design are required for its comprehensive analysis. On the other hand, the data from these studies often include a substantial amount of redundancy such as proteins that are typically represented by a multitude of peptides. Coping simultaneously with both complexities (experimental and technological) makes data analysis a challenge for Bioinformatics. Results: We present a comprehensive work-flow tailored for analyzing complex data including data from multifactorial studies. The developed approach aims at revealing effects caused by a distinct combination of experimental factors, in our case genotype and diet. Applying the developed work-flow to the analysis of an established polygenic mouse model for diet-induced type 2 diabetes, we found peptides with significant fold changes exclusively for the combination of a particular strain and diet. Exploitation of redundancy enables the visualization of peptide correlation and provides a natural way of feature selection for classification and prediction. Classification based on the features selected using our approach performs similar to classifications based on more complex feature selection methods. Conclusions: The combination of ANOVA and redundancy exploitation allows for identification of biomarker candidates in multi-dimensional MALDI-TOF MS profiling studies with complex experimental design. With respect to feature selection our method provides a fast and intuitive alternative to global optimization strategies with comparable performance. The method is implemented in R and the scripts are available by contacting the corresponding author. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
8. Association of obesity with down-regulation of heat shock protein 40 expression and evidence that exercise retrieves its normal expression.
- Author
-
Abubakr, Jehad, Abu-Farha, Mohamed, Al-Arouj, Monira, Al-Ghimlas, Fahad, Al-Khairi, Irina, Al-Mudhaf, Dalal, Baturcam, Engin, Bennakhi, Abdullah, Cherian, Preethi, Hammad, Maha, John, Jeena, Kavalakatt, Sina, Khadir, Abdelkrim, Tiss, Ali, Warsame, Samia, Dermime, Said, and Dehbi, Mohammed
- Subjects
OBESITY - Abstract
An abstract of the article "Association of obesity with down-regulation of heat shock protein 40 expression and evidence that exercise retrieves its normal expression," by Jehad Abubakr, Mohamed Abu-Farha, and Monira Al-Arouj is presented.
- Published
- 2012
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.