4 results on '"Thompson, Claudia"'
Search Results
2. Cestode strobilation: prediction of developmental genes and pathways
- Author
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Paludo, Gabriela Prado, Thompson, Claudia Elizabeth, Miyamoto, Kendi Nishino, Guedes, Rafael Lucas Muniz, Zaha, Arnaldo, de Vasconcelos, Ana Tereza Ribeiro, Cancela, Martin, and Ferreira, Henrique Bunselmeyer
- Published
- 2020
- Full Text
- View/download PDF
3. Comparative genome analysis of entomopathogenic fungi reveals a complex set of secreted proteins.
- Author
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Staats, Charley Christian, Junges, Ângela, Muniz Guedes, Rafael Lucas, Thompson, Claudia Elizabeth, de Morais, Guilherme Loss, Boldo, Juliano Tomazzoni, de Almeida, Luiz Gonzaga Paula, Andreis, Fábio Carrer, Gerber, Alexandra Lehmkuhl, Sbaraini, Nicolau, de Andrade da Paixão, Rana Louise, Broetto, Leonardo, Landell, Melissa, Santi, Lucélia, Beys-da-Silva, Walter Orlando, Silveira, Carolina Pereira, Serrano, Thaiane Rispoli, de Oliveira, Eder Silva, Kmetzsch, Lívia, and Vainstein, Marilene Henning
- Abstract
Background: Metarhizium anisopliae is an entomopathogenic fungus used in the biological control of some agricultural insect pests, and efforts are underway to use this fungus in the control of insect-borne human diseases. A large repertoire of proteins must be secreted by M. anisopliae to cope with the various available nutrients as this fungus switches through different lifestyles, i.e., from a saprophytic, to an infectious, to a plant endophytic stage. To further evaluate the predicted secretome of M. anisopliae, we employed genomic and transcriptomic analyses, coupled with phylogenomic analysis, focusing on the identification and characterization of secreted proteins. Results: We determined the M. anisopliae E6 genome sequence and compared this sequence to other entomopathogenic fungi genomes. A robust pipeline was generated to evaluate the predicted secretomes of M. anisopliae and 15 other filamentous fungi, leading to the identification of a core of secreted proteins. Transcriptomic analysis using the tick Rhipicephalus microplus cuticle as an infection model during two periods of infection (48 and 144 h) allowed the identification of several differentially expressed genes. This analysis concluded that a large proportion of the predicted secretome coding genes contained altered transcript levels in the conditions analyzed in this study. In addition, some specific secreted proteins from Metarhizium have an evolutionary history similar to orthologs found in Beauveria/Cordyceps. This similarity suggests that a set of secreted proteins has evolved to participate in entomopathogenicity. Conclusions: The data presented represents an important step to the characterization of the role of secreted proteins in the virulence and pathogenicity of M. anisopliae. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
4. New insights on the biology of swine respiratory tract mycoplasmas from a comparative genome analysis.
- Author
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Siqueira, Franciele Maboni, Thompson, Claudia Elizabeth, Virginio, Veridiana Gomes, Gonchoroski, Taylor, Reolon, Luciano, Almeida, Luiz Gonzaga, da Fonsêca, Marbella Maria, de Souza, Rangel, Prosdocimi, Francisco, Schrank, Irene Silveira, Ferreira, Henrique Bunselmeyer, and de Vasconcelos, Ana Tereza Ribeiro
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RESPIRATORY infections , *ANIMAL species , *NUCLEOTIDE sequence , *MYCOPLASMATALES , *ARTHRITIS in animals , *SWINE diseases , *BACTERIAL adhesins , *COMPARATIVE genomics - Abstract
Background: Mycoplasma hyopneumoniae, Mycoplasma flocculare and Mycoplasma hyorhinis live in swine respiratory tracts. M. flocculare, a commensal bacterium, is genetically closely related to M. hyopneumoniae, the causative agent of enzootic porcine pneumonia. M. hyorhinis is also pathogenic, causing polyserositis and arthritis. In this work, we present the genome sequences of M. flocculare and M. hyopneumoniae strain 7422, and we compare these genomes with the genomes of other M. hyoponeumoniae strain and to the a M. hyorhinis genome. These analyses were performed to identify possible characteristics that may help to explain the different behaviors of these species in swine respiratory tracts. Results: The overall genome organization of three species was analyzed, revealing that the ORF clusters (OCs) differ considerably and that inversions and rearrangements are common. Although M. flocculare and M. hyopneumoniae display a high degree of similarity with respect to the gene content, only some genomic regions display considerable synteny. Genes encoding proteins that may be involved in host-cell adhesion in M. hyopneumoniae and M. flocculare display differences in genomic structure and organization. Some genes encoding adhesins of the P97 family are absent in M. flocculare and some contain sequence differences or lack of domains that are considered to be important for adhesion to host cells. The phylogenetic relationship of the three species was confirmed by a phylogenomic approach. The set of genes involved in metabolism, especially in the uptake of precursors for nucleic acids synthesis and nucleotide metabolism, display some differences in copy number and the presence/absence in the three species. Conclusions: The comparative analyses of three mycoplasma species that inhabit the swine respiratory tract facilitated the identification of some characteristics that may be related to their different behaviors. M. hyopneumoniae and M. flocculare display many differences that may help to explain why one species is pathogenic and the other is considered to be commensal. However, it was not possible to identify specific virulence determinant factors that could explain the differences in the pathogenicity of the analyzed species. The M. hyorhinis genome contains differences in some components involved in metabolism and evasion of the host's immune system that may contribute to its growth aggressiveness. Several horizontal gene transfer events were identified. The phylogenomic analysis places M. hyopneumoniae, M. flocculare and M. hyorhinis in the hyopneumoniae clade. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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