8 results on '"Tamoufe, Ubald"'
Search Results
2. Publisher Correction To: Disclosure of same-sex practices and experiences of healthcare stigma among cisgender men who have sex with men in five sub-Saharan African countries
- Author
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Wiginton, John Mark, Murray, Sarah M., Poku, Ohemaa, Augustinavicius, Jura, Jackman, Kevon-Mark Phillip, Kane, Jeremy, Billong, Serge C., Diouf, Daouda, Ba, Ibrahima, Mothopeng, Tampose, Njindam, Iliassou Mfochive, Turpin, Gnilane, Tamoufe, Ubald, Sithole, Bhekie, Zlotorzynska, Maria, Sanchez, Travis H., and Baral, Stefan D.
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- 2022
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3. Disclosure of same-sex practices and experiences of healthcare stigma among cisgender men who have sex with men in five sub-Saharan African countries
- Author
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Wiginton, John Mark, Murray, Sarah M., Poku, Ohemaa, Augustinavicius, Jura, Jackman, Kevon-Mark Phillip, Kane, Jeremy, Billong, Serge C., Diouf, Daouda, Ba, Ibrahima, Mothopeng, Tampose, Njindam, Iliassou Mfochive, Turpin, Gnilane, Tamoufe, Ubald, Sithole, Bhekie, Zlotorzynska, Maria, Sanchez, Travis H., and Baral, Stefan D.
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- 2021
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4. Novel simian foamy virus infections from multiple monkey species in women from the Democratic Republic of Congo.
- Author
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Switzer, William M, Shaohua Tang, Ahuka-Mundeke, Steve, Shankar, Anupama, Hanson, Debra L., HaoQiang Zheng, Ayouba, Ahidjo, Wolfe, Nathan D., LeBreton, Matthew, Djoko, Cyrille F., Tamoufe, Ubald, Esteban, Amandine, Heneine, Walid, Peeters, Martine, Wright, Linda L., Muyembe-Tamfum, Jean Jacques, Wemakoy, Emile Okitolonda, Mulembakani, Prime, Hoff, Nicole A., and Rimoin, Anne W.
- Subjects
VIRUS diseases ,SIMIAN foamy virus ,INFECTIOUS disease transmission ,GENE amplification - Abstract
Background: Zoonotic transmission of simian retroviruses in Central Africa is ongoing and can result in pandemic human infection. While simian foamy virus (SFV) infection was reported in primate hunters in Cameroon and Gabon, little is known about the distribution of SFV in Africa and whether human-to-human transmission and disease occur. We screened 3,334 plasmas from persons living in rural villages in central Democratic Republic of Congo (DRC) using SFV-specific EIA and Western blot (WB) tests. PCR amplification of SFV polymerase sequences from DNA extracted from buffy coats was used to measure proviral loads. Phylogenetic analysis was used to define the NHP species origin of SFV. Participants completed questionnaires to capture NHP exposure information. Results: Sixteen (0.5%) samples were WB-positive; 12 of 16 were from women (75%, 95% confidence limits 47.6%, 92.7%). Sequence analysis detected SFV in three women originating from Angolan colobus or red-tailed monkeys; both monkeys are hunted frequently in DRC. NHP exposure varied and infected women lived in distant villages suggesting a wide and potentially diverse distribution of SFV infections across DRC. Plasmas from 22 contacts of 8 WB-positive participants were all WB negative suggesting no secondary viral transmission. Proviral loads in the three women ranged from 14 - 1,755 copies/10
5 cells. Conclusions: Our study documents SFV infection in rural DRC for the first time and identifies infections with novel SFV variants from Colobus and red-tailed monkeys. Unlike previous studies, women were not at lower risk for SFV infection in our population, providing opportunities for spread of SFV both horizontally and vertically. However, limited testing of close contacts of WB-positive persons did not identify human-to-human transmission. Combined with the broad behavioral risk and distribution of NHPs across DRC, our results suggest that SFV infection may have a wider geographic distribution within DRC. These results also reinforce the potential for an increased SFV prevalence throughout the forested regions of Africa where humans and simians co-exist. Our finding of endemic foci of SFV infection in DRC will facilitate longitudinal studies to determine the potential for person-to-person transmissibility and pathogenicity of these zoonotic retroviral infections. [ABSTRACT FROM AUTHOR]- Published
- 2012
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5. Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms.
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Etienne, Lucie, Nerrienet, Eric, LeBreton, Matthew, Bibila, Godwin Tafon, Foupouapouognigni, Yacouba, Rousset, Dominique, Nana, Ahmadou, Djoko, Cyrille F., Tamoufe, Ubald, Aghokeng, Avelin F., Mpoudi-Ngole, Eitel, Delaporte, Eric, Peeters, Martine, Wolfe, Nathan D., and Ayouba, Ahidjo
- Subjects
SIMIAN immunodeficiency virus ,CHIMPANZEES as laboratory animals ,PATHOGENIC microorganisms ,THROMBOCYTOPENIA ,GLYCOSYLATION - Abstract
Background: Data on the evolution of natural SIV infection in chimpanzees (SIVcpz) and on the impact of SIV on local ape populations are only available for Eastern African chimpanzee subspecies (Pan troglodytes schweinfurthii), and no data exist for Central chimpanzees (Pan troglodytes troglodytes), the natural reservoir of the ancestors of HIV-1 in humans. Here, we report a case of naturally-acquired SIVcpz infection in a P.troglodytes chimpanzee with clinical and biological data and analysis of viral evolution over the course of infection. Results: A male chimpanzee (Cam155), 1.5 years, was seized in southern Cameroon in November 2003 and screened SIV positive during quarantine. Clinical follow-up and biological analyses have been performed for 7 years and showed a significant decline of CD4 counts (1,380 cells/mm
³ in 2004 vs 287 in 2009), a severe thrombocytopenia (130,000 cells/mm³ in 2004 vs 5,000 cells/mm³ in 2009), a weight loss of 21.8% from August 2009 to January 2010 (16 to 12.5 kg) and frequent periods of infections with diverse pathogens. DNA from PBMC, leftover from clinical follow-up samples collected in 2004 and 2009, was used to amplify overlapping fragments and sequence two full-length SIVcpzPtt-Cam155 genomes. SIVcpzPtt-Cam155 was phylogenetically related to other SIVcpzPtt from Cameroon (SIVcpzPtt-Cam13) and Gabon (SIVcpzPtt-Gab1). Ten molecular clones 5 years apart, spanning the V1V4 gp120 env region (1,100 bp), were obtained. Analyses of the env region showed positive selection (dN-dS >0), intra-host length variation and extensive amino acid diversity between clones, greater in 2009. Over 5 years, N-glycosylation site frequency significantly increased (p < 0.0001). Conclusions: Here, we describe for the first time the clinical history and viral evolution of a naturally SIV infected P.t.troglodytes chimpanzee. The findings show an increasing viral diversity over time and suggest clinical progression to an AIDS-like disease, showing that SIVcpz can be pathogenic in its host, as previously described in P.t.schweinfurthii. Although studying the impact of SIV infection in wild apes is difficult, efforts should be made to better characterize the pathogenicity of the ancestors of HIV-1 in their natural host and to find out whether SIV infection also plays a role in ape population decline. [ABSTRACT FROM AUTHOR]- Published
- 2011
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6. HIV-1 recombinants with multiple parental strains in low-prevalence, remote regions of Cameroon: Evolutionary relics?
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Carr, Jean K., Wolfe, Nathan D., Torimiro, Judith N., Tamoufe, Ubald, Mpoudi-Ngole, E, Eyzaguirre, Lindsay, Birx, Deborah L., McCutchan, Francine E., and Burke, Donald S.
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HIV ,PANDEMICS ,DNA ,GENOMES - Abstract
Background: The HIV pandemic disseminated globally from Central West Africa, beginning in the second half of the twentieth century. To elucidate the virologic origins of the pandemic, a cross-sectional study was conducted of the genetic diversity of HIV-1 strains in villagers in 14 remote locations in Cameroon and in hospitalized and STI patients. DNA extracted from PBMC was PCR amplified from HIV(+) subjects. Partial pol amplicons (N = 164) and nearly full virus genomes (N = 78) were sequenced. Among the 3956 rural villagers studied, the prevalence of HIV infection was 4.9%; among the hospitalized and clinic patients, it was 8.6%. Results: Virus genotypes fell into two distinctive groups. A majority of the genotyped strains (109/164) were the circulating recombinant form (CRF) known to be endemic in West Africa and Central West Africa, CRF02AG. The second most common genetic form (9/164) was the recently described CRF2201A1, and the rest were a collection of 4 different subtypes (A2, D, F2, G) and 6 different CRFs (-01, -11, -13, -18, -25, -37). Remarkably, 10.4% of HIV-1 genomes detected (17/164) were heretofore undescribed unique recombinant forms (URF) present in only a single person. Nearly full genome sequencing was completed for 78 of the viruses of interest. HIV genetic diversity was commonplace in rural villages: 12 villages each had at least one newly detected URF, and 9 villages had two or more. Conclusions: These results show that while CRF02AG dominated the HIV strains in the rural villages, the remainder of the viruses had tremendous genetic diversity. Between the trans-species transmission of SIVcpz and the dispersal of pandemic HIV-1, there was a time when we hypothesize that nascent HIV-1 was spreading, but only to a limited extent, recombining with other local HIV-1, creating a large variety of recombinants. When one of those recombinants began to spread widely (i.e. became epidemic), it was recognized as a subtype. We hypothesize that the viruses in these remote Cameroon villages may represent that pre-epidemic stage of viral evolution. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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7. Capacity-building efforts by the AFHSC-GEIS program.
- Author
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Sanchez JL, Johns MC, Burke RL, Vest KG, Fukuda MM, Yoon IK, Lon C, Quintana M, Schnabel DC, Pimentel G, Mansour M, Tobias S, Montgomery JM, Gray GC, Saylors K, Ndip LM, Lewis S, Blair PJ, Sjoberg PA, Kuschner RA, Russell KL, Blazes DL, Witt CJ, Money NN, Gaydos JC, Pavlin JA, Gibbons RV, Jarman RG, Stoner M, Shrestha SK, Owens AB, Iioshi N, Osuna MA, Martin SK, Gordon SW, Bulimo WD, Waitumbi DJ, Assefa B, Tjaden JA, Earhart KC, Kasper MR, Brice GT, Rogers WO, Kochel T, Laguna-Torres VA, Garcia J, Baker W, Wolfe N, Tamoufe U, Djoko CF, Fair JN, Akoachere JF, Feighner B, Hawksworth A, Myers CA, Courtney WG, Macintosh VA, Gibbons T, Macias EA, Grogl M, O'Neil MT, Lyons AG, Houng HS, Rueda L, Mattero A, Sekonde E, Sang R, Sang W, Palys TJ, Jerke KH, Millard M, Erima B, Mimbe D, Byarugaba D, Wabwire-Mangen F, Shiau D, Wells N, Bacon D, Misinzo G, Kulanga C, Haverkamp G, Kohi YM, Brown ML, Klein TA, Meyers M, Schoepp RJ, Norwood DA, Cooper MJ, Maza JP, Reeves WE, and Guan J
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- Global Health, Government Agencies, Humans, International Cooperation, Laboratories, United States, Influenza, Human epidemiology, Military Personnel, Public Health, Respiratory Tract Infections epidemiology, Sentinel Surveillance
- Abstract
Capacity-building initiatives related to public health are defined as developing laboratory infrastructure, strengthening host-country disease surveillance initiatives, transferring technical expertise and training personnel. These initiatives represented a major piece of the Armed Forces Health Surveillance Center, Division of Global Emerging Infections Surveillance and Response System (AFHSC-GEIS) contributions to worldwide emerging infectious disease (EID) surveillance and response. Capacity-building initiatives were undertaken with over 80 local and regional Ministries of Health, Agriculture and Defense, as well as other government entities and institutions worldwide. The efforts supported at least 52 national influenza centers and other country-specific influenza, regional and U.S.-based EID reference laboratories (44 civilian, eight military) in 46 countries worldwide. Equally important, reference testing, laboratory infrastructure and equipment support was provided to over 500 field sites in 74 countries worldwide from October 2008 to September 2009. These activities allowed countries to better meet the milestones of implementation of the 2005 International Health Regulations and complemented many initiatives undertaken by other U.S. government agencies, such as the U.S. Department of Health and Human Services, the U.S. Agency for International Development and the U.S. Department of State.
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- 2011
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8. Genetic characterization of the complete genome of a highly divergent simian T-lymphotropic virus (STLV) type 3 from a wild Cercopithecus mona monkey.
- Author
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Sintasath DM, Wolfe ND, Zheng HQ, LeBreton M, Peeters M, Tamoufe U, Djoko CF, Diffo JL, Mpoudi-Ngole E, Heneine W, and Switzer WM
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- Amino Acid Sequence, Animals, Base Sequence, Chromosome Walking, Cluster Analysis, DNA, Viral chemistry, Models, Molecular, Molecular Sequence Data, Nucleic Acid Conformation, Phylogeny, Polymerase Chain Reaction methods, Sequence Homology, Simian T-lymphotropic virus 3 isolation & purification, Cercopithecus virology, DNA, Viral genetics, Deltaretrovirus Infections veterinary, Genome, Viral, Sequence Analysis, DNA, Simian T-lymphotropic virus 3 genetics, Tumor Virus Infections veterinary
- Abstract
Background: The recent discoveries of novel human T-lymphotropic virus type 3 (HTLV-3) and highly divergent simian T-lymphotropic virus type 3 (STLV-3) subtype D viruses from two different monkey species in southern Cameroon suggest that the diversity and cross-species transmission of these retroviruses are much greater than currently appreciated., Results: We describe here the first full-length sequence of a highly divergent STLV-3d(Cmo8699AB) virus obtained by PCR-based genome walking using DNA from two dried blood spots (DBS) collected from a wild-caught Cercopithecus mona monkey. The genome of STLV-3d(Cmo8699AB) is 8913-bp long and shares only 77% identity to other PTLV-3s. Phylogenetic analyses using Bayesian and maximum likelihood inference clearly show that this highly divergent virus forms an independent lineage with high posterior probability and bootstrap support within the diversity of PTLV-3. Molecular dating of concatenated gag-pol-env-tax sequences inferred a divergence date of about 115,117 years ago for STLV-3d(Cmo8699AB) indicating an ancient origin for this newly identified lineage. Major structural, enzymatic, and regulatory gene regions of STLV-3d(Cmo8699AB) are intact and suggest viral replication and a predicted pathogenic potential comparable to other PTLV-3s., Conclusion: When taken together, the inferred ancient origin of STLV-3d(Cmo8699AB), the presence of this highly divergent virus in two primate species from the same geographical region, and the ease with which STLVs can be transmitted across species boundaries all suggest that STLV-3d may be more prevalent and widespread. Given the high human exposure to nonhuman primates in this region and the unknown pathogenicity of this divergent PTLV-3, increased surveillance and expanded prevention activities are necessary. Our ability to obtain the complete viral genome from DBS also highlights further the utility of this method for molecular-based epidemiologic studies.
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- 2009
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