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Start Over Author "Sophie M. Bastarache" Publisher biomed central
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1. Latent HIV-1 can be reactivated by superinfection in a Tat-dependent manner, which can lead to the emergence of recombinant viruses

Author

Mark A. Wainberg, Sophie M. Bastarache, Daniel A. Donahue, and Richard D Sloan

Subjects
Reporter gene, biology, viruses, Cell, Mutant, virus diseases, medicine.disease_cause, Virology, Jurkat cells, law.invention, Infectious Diseases, medicine.anatomical_structure, Latent Virus, law, Superinfection, Poster Presentation, biology.protein, Recombinant DNA, medicine, Antibody
Abstract

Methods We used both Jurkat and primary cell latency models. In Jurkat cells, wt and drug-resistant latent populations were superinfected with wt or drug-resistant viruses, including nef or tat mutants, and treated with various inhibitors. Viral reporter gene expression was measured by FACS. Sequence tags on each resistant virus permitted identification of recombinants. We also performed superinfection experiments in primary cells.

Published
2013

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