32 results on '"Shen, Nan"'
Search Results
2. Predictive value of the stress hyperglycemia ratio in dialysis patients with acute coronary syndrome: insights from a multi-center observational study
- Author
-
Xie, Enmin, Ye, Zixiang, Wu, Yaxin, Zhao, Xuecheng, Li, Yike, Shen, Nan, Gao, Yanxiang, and Zheng, Jingang
- Published
- 2023
- Full Text
- View/download PDF
3. Association of triglyceride–glucose index with coronary severity and mortality in patients on dialysis with coronary artery disease
- Author
-
Xie, Enmin, Ye, Zixiang, Wu, Yaxin, Zhao, Xuecheng, Li, Yike, Shen, Nan, Guo, Xiaochun, Gao, Yanxiang, and Zheng, Jingang
- Published
- 2023
- Full Text
- View/download PDF
4. The prediction of in-hospital mortality in chronic kidney disease patients with coronary artery disease using machine learning models
- Author
-
Ye, Zixiang, An, Shuoyan, Gao, Yanxiang, Xie, Enmin, Zhao, Xuecheng, Guo, Ziyu, Li, Yike, Shen, Nan, Ren, Jingyi, and Zheng, Jingang
- Published
- 2023
- Full Text
- View/download PDF
5. Association between the triglyceride glucose index and in-hospital and 1-year mortality in patients with chronic kidney disease and coronary artery disease in the intensive care unit
- Author
-
Ye, Zixiang, An, Shuoyan, Gao, Yanxiang, Xie, Enmin, Zhao, Xuecheng, Guo, Ziyu, Li, Yike, Shen, Nan, and Zheng, Jingang
- Published
- 2023
- Full Text
- View/download PDF
6. Chronic atrophic gastritis and risk of incident upper gastrointestinal cancers: a systematic review and meta-analysis
- Author
-
Li, Junqiu, Pan, Jielu, Xiao, Dinghong, Shen, Nan, Wang, Ruiqing, Miao, Hongyv, Pu, Peimin, Zhang, Haiyan, Yv, Xiao, and Xing, Lianjun
- Published
- 2024
- Full Text
- View/download PDF
7. Anti-Toxoplasma gondii antibodies as a risk factor for the prevalence and severity of systemic lupus erythematosus
- Author
-
Li, Zhongzhen, Lei, Zhiwei, Yang, Wanying, Jing, Chunxia, Sun, Xiaolin, Yang, Guang, Zhao, Xiaozhen, Zhang, Mingjiao, Xu, Miaomiao, Tang, Yuanjia, Wang, Qingwen, Zhao, Jing, Zhou, Zixing, Wen, Zihao, Chen, Xiaojing, Peng, Qinglin, Wang, Guochun, Zhang, Pingjing, Sun, Erwei, Shen, Nan, Xu, Weiguo, Li, Zhanguo, Yang, Hengwen, and Yin, Zhinan
- Published
- 2024
- Full Text
- View/download PDF
8. Urinary galectin-3 binding protein (G3BP) as a biomarker for disease activity and renal pathology characteristics in lupus nephritis
- Author
-
Ding, Huihua, Shen, Yiwei, Lin, Cheng, Qin, Ling, He, Shijun, Dai, Min, Okitsu, Shinji L., DeMartino, Julie A., Guo, Qiang, and Shen, Nan
- Published
- 2022
- Full Text
- View/download PDF
9. Impact of viral coinfection and macrolide-resistant mycoplasma infection in children with refractory Mycoplasma pneumoniae pneumonia
- Author
-
Zhou, Yajuan, Wang, Jing, Chen, Wenjuan, Shen, Nan, Tao, Yue, Zhao, Ruike, Luo, Lijuan, Li, Biru, and Cao, Qing
- Published
- 2020
- Full Text
- View/download PDF
10. Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome
- Author
-
Cui, Jiawen, shen, Nan, Lu, Zhaogeng, Xu, Guolu, Wang, Yuyao, and Jin, Biao
- Published
- 2020
- Full Text
- View/download PDF
11. Urinary activated leukocyte cell adhesion molecule as a novel biomarker of lupus nephritis histology
- Author
-
Ding, Huihua, Lin, Cheng, Cai, Jingyi, Guo, Qiang, Dai, Min, Mohan, Chandra, and Shen, Nan
- Published
- 2020
- Full Text
- View/download PDF
12. Prognostic implications of Aquaporin 9 expression in clear cell renal cell carcinoma
- Author
-
Xu, Wen-Hao, Shi, Shen-Nan, Xu, Yue, Wang, Jun, Wang, Hong-Kai, Cao, Da-Long, Shi, Guo-Hai, Qu, Yuan-Yuan, Zhang, Hai-Liang, and Ye, Ding-Wei
- Published
- 2019
- Full Text
- View/download PDF
13. Interferon-α exacerbates neuropsychiatric phenotypes in lupus-prone mice
- Author
-
Zeng, Jing, Meng, Xinyu, Zhou, Ping, Yin, Zhihua, Xie, Qinglian, Zou, Hong, Shen, Nan, Ye, Zhizhong, and Tang, Yuanjia
- Published
- 2019
- Full Text
- View/download PDF
14. Genomic test ends a long diagnostic odyssey in a patient with resistance to thyroid hormones
- Author
-
Arsov, Todor, Xie, Chengmei, Shen, Nan, Andrews, Dan, Vinuesa, Carola G., and Vaskova, Olivija
- Published
- 2019
- Full Text
- View/download PDF
15. Selective IgA Deficiency in Autoimmune Diseases
- Author
-
Wang, Ning, Shen, Nan, Vyse, Timothy J., Anand, Vidya, Gunnarson, Iva, Sturfelt, Gunnar, Rantapää-Dahlqvist, Solbritt, Elvin, Kerstin, Truedsson, Lennart, Andersson, Bengt A., Dahle, Charlotte, Örtqvist, Eva, Gregersen, Peter K., Behrens, Timothy W., and Hammarström, Lennart
- Published
- 2011
- Full Text
- View/download PDF
16. microRNA-mediated regulation of innate immune response in rheumatic diseases
- Author
-
Xiaobing Luo, Ranade, Koustubh, Talker, Ronel, Jallal, Bahija, Shen, Nan, and Yihong Yao
- Published
- 2013
- Full Text
- View/download PDF
17. Genomic Signatures Characterize Leukocyte Infiltration in Myositis Muscles
- Author
-
Zhu, Wei, Streicher, Katie, Shen, Nan, Higgs, Brandon W, Morehouse, Chris, Greenlees, Lydia, Amato, Anthony A., Ranade, Koustubh, Richman, Laura, Fiorentino, David, Jallal, Bahija, Greenberg, Steven A., and Yao, Yihong
- Subjects
Myositis ,Genomics ,Leukocyte infiltration ,Type 1 interferon ,miR-146a - Abstract
Background: Leukocyte infiltration plays an important role in the pathogenesis and progression of myositis, and is highly associated with disease severity. Currently, there is a lack of: efficacious therapies for myositis; understanding of the molecular features important for disease pathogenesis; and potential molecular biomarkers for characterizing inflammatory myopathies to aid in clinical development. Methods: In this study, we developed a simple model and predicted that 1) leukocyte-specific transcripts (including both protein-coding transcripts and microRNAs) should be coherently overexpressed in myositis muscle and 2) the level of over-expression of these transcripts should be correlated with leukocyte infiltration. We applied this model to assess immune cell infiltration in myositis by examining mRNA and microRNA (miRNA) expression profiles in muscle biopsies from 31 myositis patients and 5 normal controls. Results: Several gene signatures, including a leukocyte index, type 1 interferon (IFN), MHC class I, and immunoglobulin signature, were developed to characterize myositis patients at the molecular level. The leukocyte index, consisting of genes predominantly associated with immune function, displayed strong concordance with pathological assessment of immune cell infiltration. This leukocyte index was subsequently utilized to differentiate transcriptional changes due to leukocyte infiltration from other alterations in myositis muscle. Results from this differentiation revealed biologically relevant differences in the relationship between the type 1 IFN pathway, miR-146a, and leukocyte infiltration within various myositis subtypes. Conclusions: Results indicate that a likely interaction between miR-146a expression and the type 1 IFN pathway is confounded by the level of leukocyte infiltration into muscle tissue. Although the role of miR-146a in myositis remains uncertain, our results highlight the potential benefit of deconvoluting the source of transcriptional changes in myositis muscle or other heterogeneous tissue samples. Taken together, the leukocyte index and other gene signatures developed in this study may be potential molecular biomarkers to help to further characterize inflammatory myopathies and aid in clinical development. These hypotheses need to be confirmed in separate and sufficiently powered clinical trials.
- Published
- 2012
- Full Text
- View/download PDF
18. Distribution of ischemic infarction and stenosis of intra- and extracranial arteries in young Chinese patients with ischemic stroke.
- Author
-
Ojha, Rajeev, Huang, Dongya, An, Hedi, Liu, Rong, Du, Cui, Shen, Nan, Tu, Zhilan, and Li, Ying
- Abstract
Background: The distribution of cerebral ischemic infarction and stenosis in ischemic stroke may vary with age-group, race and gender. This study was conducted to understand the risk factors and characteristics of cerebral infarction and stenosis of vessels in young Chinese patients with ischemic stroke.Methods: This was a retrospective study, from January 2007 to July 2012, of 123 patients ≤50 years diagnosed with acute ischemic stroke. Patient characteristics were compared according to sex (98 males and 25 females) and age group (51 patients were ≤45 years and 72 patients were 46-50 years). Characteristics of acute ischemic infarction were studied by diffusion weighted imaging. Stenosis of intra- and extracranial arteries was diagnosed by duplex sonography, head magnetic resonance angiography (MRA) or cervical MRA.Results: Common risk factors were hypertension (72.4 %), dyslipidemia (55.3 %), smoking (54.4 %) and diabetes (33.3 %). Lacunar Infarction was most common in our patients (41.5 %). Partial anterior circulation infarction was predominant in females (52.0 vs 32.7 %; P = 0.073) and posterior circulation infarction in males (19.8 vs 4 %; P = 0.073). Multiple brain infarctions were found in 38 patients (30.9 %). Small artery atherosclerosis was found in 54 patients (43.9 %), with higher prevalence in patients of the 46-50 years age-group. Intracranial stenosis was more common than extracranial stenosis, and middle cerebral artery stenosis was most prevalent (27.3 %). Stenosis in the anterior circulation was more frequent than in the posterior circulation (P < 0.001).Conclusions: In these young patients, hypertension, smoking, dyslipidemia and diabetes were common risk factors. Intracranial stenosis was most common. The middle cerebral artery was highly vulnerable. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF
19. Osteogenic differentiation of mesenchymal stem cells promotes c-Jun-dependent secretion of interleukin 8 and mediates the migration and differentiation of CD4 + T cells.
- Author
-
Ye F, Li J, Xu P, Xie Z, Zheng G, Liu W, Ye G, Yu W, Lin J, Su Z, Che Y, Zhang Z, Wang P, Wu Y, and Shen H
- Subjects
- CD4-Positive T-Lymphocytes, Cell Differentiation physiology, Cell Movement, Cells, Cultured, Humans, Interleukin-8 metabolism, Proto-Oncogene Proteins c-jun, Mesenchymal Stem Cells metabolism, Osteogenesis
- Abstract
Background: The immune system and the skeletal system have complex interactions in the bone marrow and even in the joints, which has promoted the development of the concept of osteoimmunology. Some evidence has indicated that T cells and B cells contribute to the balance between the resorption and formation of bone. However, there has been little discussion on the regulation of CD4
+ T lymphocytes by cells involved in bone metabolism. Mesenchymal stem cells (MSCs), which exert core functions related to immunoregulation and osteogenic differentiation, are crucial cells linked to both bone metabolism and the immune system. Previous studies have shown that the immunoregulatory capacity of MSCs changes following differentiation. However, it is still unclear whether the osteogenic differentiation of MSCs affects the migration and differentiation of CD4+ T cells., Methods: MSCs were cultured in growth medium or osteogenic medium for 10 days and then cocultured with CD4+ T cells. CD4+ T cell migration and differentiation were detected by flow cytometry. Further, gene expression levels of specific cytokines were analyzed by quantitative real-time PCR and enzyme-linked immunosorbent assays. A Proteome Profiler Human XL Cytokine Array Kit was used to analyze supernatants collected from MSCs. Alizarin red S staining and Alkaline phosphatase assay were used to detect the osteogenic differentiation of MSCs., Results: Here, we found that the migration of CD4+ T cells was elevated, and the capacity to induce the differentiation of regulatory T (Treg) cells was weakened during MSC osteogenic differentiation, while the differentiation of T helper 1 (Th1), T helper 2 (Th2) and T helper 17 (Th17) cells was not affected. Further studies revealed that interleukin (IL)-8 was significantly upregulated during MSC osteogenic differentiation. Both a neutralizing antibody and IL-8-specific siRNA significantly inhibited the migration of CD4+ T cells and promoted the differentiation of Treg cells. Finally, we found that the transcription factor c-Jun was involved in regulating the expression of IL-8 and affected the osteogenic differentiation of MSCs, thereby mediating the migration and differentiation of CD4+ T cells., Conclusion: This study demonstrated that MSC osteogenic differentiation promoted c-Jun-dependent secretion of IL-8 and mediated the migration and differentiation of CD4+ T cells. These results provide a further understanding of the crosstalk between bone and the immune system and reveal information about the relationship between osteogenesis and inflammation in the field of osteoimmunology., (© 2022. The Author(s).)- Published
- 2022
- Full Text
- View/download PDF
20. Association between growth differentiation factor 5 rs143383 genetic polymorphism and the risk of knee osteoarthritis among Caucasian but not Asian: a meta-analysis.
- Author
-
Peng L, Jin S, Lu J, Ouyang C, Guo J, Xie Z, Shen H, and Wang P
- Subjects
- Asian People genetics, Case-Control Studies, China, Genetic Predisposition to Disease genetics, Humans, Polymorphism, Single Nucleotide genetics, Growth Differentiation Factor 5 genetics, Osteoarthritis, Knee genetics
- Abstract
Background: A few months ago, the Bioscience Reports journal showed that growth differentiation factor 5 (GDF5) rs143383 genetic polymorphism increases the susceptibility of knee osteoarthritis (KOA), but previous studies' results have debates about available data. Considering the availability of more recent data, we focus on clarifying the relationship of KOA and GDF5 rs143383 genetic polymorphism by a meta-analysis of case-control trial data., Methods: The eligible studies from the time of database established to Oct. 2019 were collected from PubMed, Springer, Cochrane library, Web of Science, China National Knowledge Infrastructure (CNKI), and Wan Fang library. Odds ratios (OR) and 95% confidence intervals (CI) were used to estimate the association between these polymorphisms and KOA risk. The meta-analysis was completed by STATA 18.0 software., Results: A total of 196 studies were collected, 16 of them included in final meta-analysis (7997 cases and 12,684 controls). There was significant association between GDF5 rs143383 polymorphism and KOA in all genetic models (for Allele model (C versus T): OR = 0.84 (95% CI = 0.76-0.91); dominate model (CC+CT versus TT): OR = 0.80 (95% CI = 0.72-0.90); recessive model (CC versus CT+TT): OR = 0.79 (95% CI = 0.68-0.92); heterozygote model (CT versus CC+TT): OR = 0.89 (95% CI = 0.80-0.97); homozygous model (CC versus TT): OR = 0.71 (95% CI = 0.60-0.85)). In the subgroup analysis, we obtained the results that there is no significance among Asians., Conclusion: GDF5 rs143383 genetic polymorphism increases the risk of KOA among Caucasians; CC genotype and C allele are protective factors for the susceptibility of KOA among Caucasians.
- Published
- 2020
- Full Text
- View/download PDF
21. Autophagy enhances mesenchymal stem cell-mediated CD4 + T cell migration and differentiation through CXCL8 and TGF-β1.
- Author
-
Cen S, Wang P, Xie Z, Yang R, Li J, Liu Z, Wang S, Wu X, Liu W, Li M, Tang S, Shen H, and Wu Y
- Subjects
- Adult, CD4-Positive T-Lymphocytes immunology, CD4-Positive T-Lymphocytes metabolism, Cells, Cultured, Female, Humans, Male, Mesenchymal Stem Cells immunology, Mesenchymal Stem Cells metabolism, T-Lymphocytes, Regulatory immunology, Young Adult, Autophagy, CD4-Positive T-Lymphocytes cytology, Cell Differentiation, Cell Movement, Interleukin-8 metabolism, Mesenchymal Stem Cells cytology, Transforming Growth Factor beta1 metabolism
- Abstract
Background: Mesenchymal stem cells (MSCs) have been recognized as a promising tool for the treatment of various inflammatory disorders and autoimmune diseases. Stress conditions affect immune-mediated treatment and activate autophagy in MSCs. However, whether autophagy affects the MSC-mediated recruitment and differentiation of CD4
+ T cells remains elusive., Methods: MSCs were pretreated with 3-methyladenine (3-MA) and rapamycin to regulate autophagy, and then co-cultured with CD4+ T cells. CD4+ T cell migration and differentiation were detected by flow cytometry. Further, gene expression levels of well-known chemokines were analyzed by quantitative real-time PCR. Enzyme-linked immunosorbent assays and western blot analysis were performed to detect C-X-C motif chemokine ligand 8 (CXCL8) and transforming growth factor (TGF)-β1 protein levels. An exogenous antibody and short hairpin RNA were used to regulate CXCL8 and TGF-β1 levels, which enabled us to evaluate how autophagy affected MSC-mediated CD4+ T cell migration and differentiation., Results: 3-MA inhibited autophagy in MSCs, which was activated by rapamycin. Rapamycin increased the migration of CD4+ T cells, whereas 3-MA decreased their migration. Mechanistically, we found that autophagy strengthened CXCL8 secretion, and the addition of exogenous CXCL8 and an anti-CXCL8 antibody eliminated the difference of CD4+ T cell migration among groups. Further, the ratio of regulatory T (Treg) cells was increased in rapamycin-pretreated MSCs, but the ratio of T helper 1 (Th1) cells was decreased, while pretreatment of MSCs with 3-MA induced the opposite effect compared with the control group. TGF-β1 overexpression and knockdown using lentiviruses rectified the differences in the ratios of Treg and Th1 cells among the groups., Conclusion: This study demonstrates that autophagy of mesenchymal stem cells mediates CD4+ T cell migration and differentiation through CXCL8 and TGF-β1, respectively. These results provide a potential new strategy for improving MSC-mediated therapy.- Published
- 2019
- Full Text
- View/download PDF
22. T-bet + CD11c + B cells are critical for antichromatin immunoglobulin G production in the development of lupus.
- Author
-
Liu Y, Zhou S, Qian J, Wang Y, Yu X, Dai D, Dai M, Wu L, Liao Z, Xue Z, Wang J, Hou G, Ma J, Harley JB, Tang Y, and Shen N
- Subjects
- Adult, Aged, Animals, Autoantibodies immunology, Autoantigens immunology, CD11c Antigen immunology, Female, Graft vs Host Disease, Humans, Immunoglobulin G immunology, Mice, Middle Aged, T-Box Domain Proteins immunology, Autoantibodies biosynthesis, B-Lymphocyte Subsets immunology, Immunoglobulin G biosynthesis, Lupus Erythematosus, Systemic immunology
- Abstract
Background: A hallmark of systemic lupus erythematosus is high titers of circulating autoantibodies. Recently, a novel CD11c
+ B-cell subset has been identified that is critical for the development of autoimmunity. However, the role of CD11c+ B cells in the development of lupus is unclear. Chronic graft-versus-host disease (cGVHD) is a lupus-like syndrome with high autoantibody production. The purpose of this study was to explore the role of CD11c+ B cells in the pathogenesis of lupus in cGVHD mice., Methods: cGVHD was induced by an intraperitoneal injection of 5 × 107 Bm12 splenocytes into B6 mice. Flow cytometry was used to analyze mice splenocytes and human samples. Magnetic beads were used to isolate mice B cells. Gene expression was determined by real-time quantitative polymerase chain reaction (RT-qPCR). Enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies in serum and supernatants., Results: The percentage and absolute number of CD11c+ B cells was increased in cGVHD-induced lupus, with elevated levels of antichromatin immunoglobulin (Ig)G and IgG2a in sera. CD11c+ plasma cells from cGVHD mice produced large amounts of antichromatin IgG2a upon stimulation. Depletion of CD11c+ B cells reduced antichromatin IgG and IgG2a production. T-bet was upregulated in CD11c+ B cells. Knockout of T-bet in B cells alleviated cGVHD-induced lupus. Importantly, the percentage of T-bet+ CD11c+ B cells increased in lupus patients and positively correlated with serum antichromatin levels., Conclusion: T-bet+ CD11c+ B cells promoted high antichromatin IgG production in the lupus-like disease model cGVHD. In lupus patients, the percentage of T-bet+ CD11c+ B cells was elevated and positively correlated with antichromatin antibodies. The findings provide potential therapeutic insight into lupus disease treatment.- Published
- 2017
- Full Text
- View/download PDF
23. Association of large intergenic noncoding RNA expression with disease activity and organ damage in systemic lupus erythematosus.
- Author
-
Wu Y, Zhang F, Ma J, Zhang X, Wu L, Qu B, Xia S, Chen S, Tang Y, and Shen N
- Subjects
- Adolescent, Adult, Aged, Biomarkers blood, Female, Humans, Lupus Erythematosus, Systemic blood, Male, Middle Aged, Real-Time Polymerase Chain Reaction, Young Adult, Lupus Erythematosus, Systemic genetics, Lupus Erythematosus, Systemic pathology, RNA, Long Noncoding genetics
- Abstract
Introduction: Despite growing evidence that large intergenic noncoding RNAs (lincRNAs) can regulate gene expression and widely take part in normal physiological and disease conditions, our knowledge of systemic lupus erythematosus (SLE)-related lincRNAs remains limited. The aim of this study was to detect the levels of four lincRNAs (ENST00000500949: linc0949, ENST00000500597: linc0597, ENST00000501992: linc1992, and ENST00000523995: linc3995) involved in innate immunity in the peripheral blood mononuclear cells (PBMCs) of patients with SLE and correlate these lincRNA levels with disease activity, organ damage, clinical features and medical therapies., Methods: PBMCs were obtained from 102 patients with SLE, 54 patients with rheumatoid arthritis (RA) and 76 healthy donors. lincRNA expression levels were measured by real-time quantitative polymerase chain reaction. Disease activity was assessed using the Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) scores, and organ damage was evaluated with the Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index., Results: linc0949 and linc0597 were significantly decreased in patients with SLE compared with patients with RA and healthy control subjects. linc0949 was correlated with SLEDAI-2K score (r = -0.329, P = 0.0007), as well as with complement component C3 level (r = 0.348, P = 0.0003). The level of linc0949 was also reduced in patients with SLE who had the presence of cumulative organ damage. In addition, decreasing expression of linc0949 was associated with lupus nephritis. linc0949 expression significantly increased after treatment, whereas neither disease activity nor organ damage correlated with linc0597 expression., Conclusions: Our results provide novel empirical evidence that linc0949 could be a potential biomarker for diagnosis, disease activity and therapeutic response in SLE.
- Published
- 2015
- Full Text
- View/download PDF
24. Generation and characterization of a new mammalian cell line continuously expressing virus-like particles of Japanese encephalitis virus for a subunit vaccine candidate.
- Author
-
Hua RH, Li YN, Chen ZS, Liu LK, Huo H, Wang XL, Guo LP, Shen N, Wang JF, and Bu ZG
- Subjects
- Animals, Antibodies, Neutralizing immunology, Antibodies, Neutralizing metabolism, Cell Line, Cricetinae, Female, Japanese Encephalitis Vaccines biosynthesis, Japanese Encephalitis Vaccines genetics, Japanese Encephalitis Vaccines immunology, Membrane Glycoproteins genetics, Membrane Glycoproteins metabolism, Mice, Mice, Inbred BALB C, Recombinant Proteins biosynthesis, Recombinant Proteins genetics, Vaccines, Virus-Like Particle biosynthesis, Vaccines, Virus-Like Particle genetics, Viral Envelope Proteins genetics, Viral Envelope Proteins metabolism, Viral Matrix Proteins genetics, Viral Matrix Proteins metabolism, Encephalitis Virus, Japanese metabolism, Vaccines, Virus-Like Particle immunology
- Abstract
Background: Japanese encephalitis virus (JEV) is the most important cause of epidemic encephalitis in most Asian regions. There is no specific treatment available for Japanese encephalitis, and vaccination is the only effective way to prevent JEV infection in humans and domestic animals. The purpose of this study is to establish a new mammalian cell line stably and efficiently expressing virus-like particle of JEV for potential use of JEV subunit vaccine., Results: We generated a new cell clone (BJ-ME cells) that stably produces a secreted form of Japanese encephalitis virus (JEV) virus-like particle (VLP). The BJ-ME cells were engineered by transfecting BHK-21 cells with a code-optimized cDNA encoding JEV prM and E protein expression plasmid. Cell line BJ-ME can stably produces a secreted form of Japanese encephalitis virus virus-like particle (JEV-VLP) which contains the JEV envelope glycoprotein (E) and membrane protein (M). The amount of JEV-VLP antigen released into the culture fluid of BJ-ME cells was as high as 15-20 μg/ml. JEV-VLP production was stable after multiple cell passages and 100% cell expression was maintained without detectable cell fusion or apoptosis. Cell culture fluid containing the JEV-VLP antigen could be harvested five to seven times continuously at intervals of 4-6 days while maintaining the culture. Mice immunized with the JEV-VLP antigen with or without adjuvant developed high titers of neutralizing antibodies and 100% protection against lethal JEV challenge., Conclusion: These results suggest that the recombinant JEV-VLP antigen produced by the BJ-ME cell line is an effective, safe and affordable subunit Japanese encephalitis vaccine candidate, especially for domestic animals such as pig and horse.
- Published
- 2014
- Full Text
- View/download PDF
25. microRNA-mediated regulation of innate immune response in rheumatic diseases.
- Author
-
Luo X, Ranade K, Talker R, Jallal B, Shen N, and Yao Y
- Subjects
- Animals, Humans, Immunity, Innate immunology, Immunity, Innate genetics, MicroRNAs immunology, Rheumatic Diseases genetics, Rheumatic Diseases immunology
- Abstract
miRNAs have been shown to play essential regulatory roles in the innate immune system. They function at multiple levels to shape the innate immune response and maintain homeostasis by direct suppression of the expression of their target proteins, preferentially crucial signaling components and transcription factors. Studies in humans and in disease models have revealed that dysregulation of several miRNAs such as miR-146a and miR-155 in rheumatic diseases leads to aberrant production of and/or signaling by inflammatory cytokines and, thus, critically contributes to disease pathogenesis. In addition, the recent description of the role of certain extracellular miRNAs as innate immune agonist to induce inflammatory response would have direct relevance to rheumatic diseases.
- Published
- 2013
- Full Text
- View/download PDF
26. A genetic role for macrophage migration inhibitory factor (MIF) in adult-onset Still's disease.
- Author
-
Wang FF, Huang XF, Shen N, Leng L, Bucala R, Chen SL, and Lu LJ
- Subjects
- Adolescent, Adult, Aged, DNA Methylation, Enzyme-Linked Immunosorbent Assay, Female, Haplotypes, Humans, Intramolecular Oxidoreductases blood, Macrophage Migration-Inhibitory Factors blood, Male, Middle Aged, Polymorphism, Restriction Fragment Length, Polymorphism, Single Nucleotide, Promoter Regions, Genetic genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Still's Disease, Adult-Onset blood, Young Adult, Genetic Predisposition to Disease genetics, Intramolecular Oxidoreductases genetics, Macrophage Migration-Inhibitory Factors genetics, Still's Disease, Adult-Onset genetics
- Abstract
Introduction: Adult-onset still's disease (AOSD) is a rare systemic inflammatory disorder in which abnormalities in inflammatory cytokines production appear to play a pathophysiological role. Our previous work has reported increased expression of macrophage migration inhibitory factor (MIF) and revealed its correlation with disease severity and activity in AOSD. A -173 G/C single nucleotide polymorphism (SNP) (rs755622) and a -794 CATT₅₋₈ repeat (rs5844572) in the MIF promoter have been reported. In this study, we sought to explore the relationship between functional MIF promoter polymorphisms and MIF expression in AOSD., Methods: 100 patients and 200 controls were recruited in the study. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was utilized to analyze the -173 G/C SNP (rs755622) and PCR-based size discrimination assay was applied to detect the -794 CATT₅₋₈ repeat (rs5844572) in the MIF promoter. Plasma MIF levels were measured by ELISA. MIF mRNA levels were quantified by real-time reverse transcription (RT)-PCR. Bisulfate genomic sequencing was employed to evaluate DNA methylation status within the MIF promoter., Results: We identified that the frequencies of MIF -794 CATT₅ (P = 0.001) allele and the expression of MIF (P <0.001) were increased in patients compared to healthy controls. Plasma levels of MIF in patients with CC genotype were higher than those of patients with GC or GG genotypes (P = 0.05). In patients with established AOSD, a higher frequency of -794 CATT₇ containing MIF genotypes was observed in those with liver dysfunction (P = 0.009). Haplotype analysis revealed a higher representation of the MIF haplotype defined by -173*C/-794 CATT₅ (C5) in AOSD patients (P = 0.001)., Conclusion: Functional promoter polymorphisms in the MIF gene influence plasma MIF levels in AOSD and may contribute to disease susceptibility or clinical presentation of AOSD.
- Published
- 2013
- Full Text
- View/download PDF
27. New insights into the role and mechanism of macrophage migration inhibitory factor in steroid-resistant patients with systemic lupus erythematosus.
- Author
-
Wang FF, Zhu LA, Zou YQ, Zheng H, Wilson A, Yang CD, Shen N, Wallace DJ, Weisman MH, Chen SL, and Lu LJ
- Subjects
- Adult, Blotting, Western, Electrophoretic Mobility Shift Assay, Enzyme-Linked Immunosorbent Assay, Female, Glucocorticoids therapeutic use, Humans, I-kappa B Kinase metabolism, Intramolecular Oxidoreductases analysis, Leukocytes, Mononuclear metabolism, Lupus Erythematosus, Systemic drug therapy, Macrophage Migration-Inhibitory Factors analysis, Male, NF-kappa B metabolism, RNA, Small Interfering, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction physiology, Transfection, Drug Resistance physiology, Intramolecular Oxidoreductases metabolism, Lupus Erythematosus, Systemic metabolism, Macrophage Migration-Inhibitory Factors metabolism
- Abstract
Introduction: Glucocorticoid (GC) therapy remains important in improving the prognosis of patients with systemic lupus erythematosus (SLE). However, some patients do not achieve an effective response with GC treatment, creating an obstacle to the remission of SLE. Identification of the underlying mechanisms responsible for steroid resistance can be significant. Macrophage migration inhibitory factor (MIF) arouses our interest because of its reciprocal relationship with GCs. In the present study, we investigated for the first time whether MIF correlated with steroid resistance in SLE and explored potential mechanisms of action., Methods: Sixty-two patients with SLE (40 steroid sensitive and 22 steroid resistant) and 21 normal controls were recruited. Serum levels of MIF were measured by ELISA. Cytosolic MIF and IκB expression in peripheral blood mononuclear cells (PBMCs) were determined by western blotting. The electrophoretic mobility shift assay was assessed by NF-κB in nuclear aliquots. Gene silencing was applied to reduce expression of MIF in PBMCs in steroid-resistant patients. PBMCs obtained from steroid-sensitive patients were treated with recombinant human MIF of different concentrations., Results: MIF levels in serum and PBMCs were higher in steroid-resistant patients compared with steroid-sensitive patients and controls. In contrast to the steroid-sensitive group, NF-κB levels were significantly higher and IκB levels lower in steroid-resistant patients. After MIF gene silencing, IκB levels in cells from steroid-resistant patients were increased. In steroid-sensitive patients, a decrease in IκB levels and an increase in NF-κB expression from baseline were detected in PBMCs treated with a higher concentration of recombinant human MIF. Treatment with recombinant human MIF did not regulate expression of IκB and NF-κB in PBMCs from patients treated with an anti-MIF monoclonal antibody., Conclusions: Our results indicated that MIF may play a role in the formation of steroid resistance in SLE by affecting the NF-κB/IκB signaling cascade. As a regulator of glucocorticoid sensitivity, MIF may be a potential target for steroid sparing.
- Published
- 2012
- Full Text
- View/download PDF
28. Single-nucleotide polymorphisms and haplotype of CYP2E1 gene associated with systemic lupus erythematosus in Chinese population.
- Author
-
Liao LH, Zhang H, Lai MP, Chen SL, Wu M, and Shen N
- Subjects
- Adult, Case-Control Studies, Female, Genetics, Population, Haplotypes, Humans, Male, Polymerase Chain Reaction, Young Adult, Asian People genetics, Cytochrome P-450 CYP2E1 genetics, Genetic Predisposition to Disease genetics, Lupus Erythematosus, Systemic genetics, Polymorphism, Single Nucleotide
- Abstract
Introduction: Cytochrome P-450 2E1 (CYP2E1) is an important member of the CYP superfamily, which is involved in the metabolism and activation of many low molecular weight toxic compounds. We tried to investigate the possible association of CYP2E1 tag single nucleotide polymorphisms (SNPs) with susceptibility to systemic lupus erythematosus (SLE) in a Chinese Han population., Methods: The coding and flanking regions of the CYP2E1 gene were scanned for polymorphisms and tag SNPs were selected. A two-stage case-control study was performed to genotype a total of 876 SLE patients and 680 geographically matched healthy controls (265 cases and 288 controls in stage I and 611 cases and 392 controls in stage II). SLE associations of alleles, genotypes and haplotypes were tested by age and sex adjusted logistic regression. The gene transcription quantitation was carried out for peripheral blood mononuclear cell (PBMC) samples from 120 healthy controls., Results: Tag SNP rs2480256 was found significantly associated with SLE in both stages of the study. The "A" allele was associated with slightly higher risk (odds ratio (OR) = 1.165, 95% confidence interval (CI) 1.073 to 1.265, P = 2.75E-4) and "A/A" genotype carriers were with even higher SLE risk (OR = 1.464 95% CI 1.259 to 1.702, P = 7.48E-7). When combined with another tag SNP rs8192772, we identified haplotype "rs8192772-rs2480256/TA" over presented in SLE patients (OR 1.407, 95% CI 1.182 to 1.675, P = 0.0001) and haplotype "TG" over presented in the controls (OR 0.771, 95% CI 0.667 to 0.890, P = 0.0004). The gene transcription quantitation analysis further proved the dominant effect of rs2480256 as the "A/A" genotype showed highest transcription., Conclusions: Our results suggest the involvement of CYP2E1 as a susceptibility gene for SLE in the Chinese population.
- Published
- 2011
- Full Text
- View/download PDF
29. Characterization of monoclonal antibodies against Muscovy duck reovirus sigmaB protein.
- Author
-
Liu M, Chen X, Wang Y, Zhang Y, Li Y, Wang Y, Shen N, and Chen H
- Subjects
- Animals, Antibodies, Monoclonal analysis, Antibodies, Viral analysis, Blotting, Western, Chlorocebus aethiops, Ducks, Enzyme-Linked Immunosorbent Assay, Mice, Mice, Inbred BALB C, Poultry Diseases virology, Reoviridae Infections immunology, Reoviridae Infections virology, Vero Cells, Antibodies, Monoclonal immunology, Antibodies, Viral immunology, Capsid Proteins immunology, Orthoreovirus, Avian immunology, Poultry Diseases immunology, RNA-Binding Proteins immunology, Reoviridae Infections veterinary
- Abstract
Background: The sigmaB protein of Muscovy duck reovirus (DRV), one of the major structural proteins, is able to induce neutralizing antibody in ducks, but the monoclonal antibody (MAb) against sigmaB protein has never been characterized., Results: Four hybridoma cell lines secreting anti-DRV sigmaB MAbs were obtained, designated 1E5, 2F7, 4E3 and 5D8. Immunoglobulin subclass tests differentiated them as IgG2b (1E5 and 4E3) and IgM (2F7 and 5D8). Dot blot and western blotting assays showed that MAbs reacted with His-sigmaB protein in a conformation-independent manner. Competitive binding assay indicated that the MAbs delineated two epitopes, A and B of sigmaB. Immunofluorescence assay indicated that the four MAbs could specifically bind to Vero cells infected with DRV and sigmaB was distributed diffusely in the cytoplasma of infected cells. MAbs had universal reactivity to all DRVs tested in an antigen-capture enzyme-linked immunosorbent assay., Conclusion: Results of this research provide important information about the four monoclonal antibodies and therefore the MAbs may be useful candidate for the development of a MAb capture ELISA for rapid detection of DRVs. In addition, it showed that the sigmaB protein was located in the cytoplasma of infected cells by immunofluorescence assay with MAbs. Virus isolation and RT-PCR are reliable way for detection of DRV infection, but these procedures are laborious, time consuming, and requiring instruments. These obvious diagnosis problems highlight the ongoing demand of rapid, reproducible, and automatic methods for the sensitive detection of DRV.
- Published
- 2010
- Full Text
- View/download PDF
30. Interferon-induced protein IFIT4 is associated with systemic lupus erythematosus and promotes differentiation of monocytes into dendritic cell-like cells.
- Author
-
Huang X, Shen N, Bao C, Gu Y, Wu L, and Chen S
- Subjects
- Adult, Autoantibodies metabolism, Case-Control Studies, Cells, Cultured, Dendritic Cells cytology, Female, Humans, Interferon-gamma metabolism, Interleukin-12 metabolism, Intracellular Signaling Peptides and Proteins genetics, Leukocytes, Mononuclear cytology, Lupus Erythematosus, Systemic etiology, Lupus Erythematosus, Systemic pathology, Male, Monocytes cytology, Plasmids, RNA, Messenger metabolism, T-Lymphocytes, Helper-Inducer pathology, Transfection, Cell Differentiation physiology, Dendritic Cells metabolism, Intracellular Signaling Peptides and Proteins metabolism, Leukocytes, Mononuclear metabolism, Lupus Erythematosus, Systemic metabolism, Monocytes metabolism
- Abstract
Introduction: Using oligonucleotide microarray, many IFN-inducible genes have been found to be highly expressed in peripheral blood mononuclear cells (PBMCs) from most patients with systemic lupus erythematosus (SLE). Among these IFN-inducible genes, IFN-induced protein with tetratricopeptide repeats 4 (IFIT4) is a novel gene whose function is unknown., Methods: In this study we examined the role played by IFIT4 in monocyte differentiation and the correlation between IFIT4 expression and the clinical manifestation of SLE. To this end, we used plasmid transfection, flow cytometry, mixed leucocyte responses, ELISA, quantitative RT-PCR and Western blotting., Results: We found that both IFIT4 mRNA and protein expression levels were significantly higher in PBMCs and monocytes from SLE patients than in those from healthy control individuals. IFIT4 expression was positively correlated with antinuclear antibodies, anti-double-stranded DNA, and anti-Sm auto-immune antibodies in SLE. Patients with SLE exhibiting higher expression of IFIT4 had a higher prevalence of leucopenia, thrombocytopenia and C3/C4 decrease. IFIT4 protein was localized exclusively to the cytoplasm, and it was significantly upregulated by IFN-alpha in normal PBMCs. To determine the role played by IFIT4 in monocyte differentiation, the monocytic cell line THP-1 was transfected with pEGFP-IFIT4 expression plasmid and stimulated with granulocyte-macrophage colony-stimulating factor/IL-4 to generate IFIT4-primed dendritic cell-like cells (DCLCs). IFIT4-primed DCLCs acquired morphological characteristics of dendritic cells more quickly, with greater resemblance to dendritic cells, as compared with DCLCs primed with pEGFP-C1 control plasmid trasfection. Furthermore, they exhibited higher expressions of CD40, CD86, CD80, HLA-DR and CD83, along with lower expression of CD14; increased IL-12 secretion; and an increased ability to stimulate T-cell proliferation. In addition, IFIT4-primed DCLCs enhanced IFN-gamma secretion (about 2.4-fold) by T cells compared with controls., Conclusion: Our findings suggest that IFIT4 might play roles in promoting monocyte differentiation into DCLCs and in directing DCLCs to modulate T-helper-1 cell differentiation; these actions might contribute to the autoimmunity and pathogenesis of SLE.
- Published
- 2008
- Full Text
- View/download PDF
31. T-614, a novel immunomodulator, attenuates joint inflammation and articular damage in collagen-induced arthritis.
- Author
-
Du F, Lü LJ, Fu Q, Dai M, Teng JL, Fan W, Chen SL, Ye P, Shen N, Huang XF, Qian J, and Bao CD
- Subjects
- Animals, Arthritis, Experimental blood, Benzopyrans pharmacology, Cartilage, Articular drug effects, Drug Therapy, Combination, Female, Knee Joint drug effects, Knee Joint immunology, Knee Joint pathology, Methotrexate therapeutic use, Rats, Rats, Wistar, Sulfonamides pharmacology, Arthritis, Experimental pathology, Arthritis, Experimental prevention & control, Benzopyrans therapeutic use, Cartilage, Articular immunology, Cartilage, Articular pathology, Inflammation Mediators therapeutic use, Sulfonamides therapeutic use
- Abstract
Introduction: T-614 is a novel oral antirheumatic agent for the treatment of rheumatoid arthritis. Whether it has immunomodulatory or disease-modifying properties and its mechanism of action are largely undetermined., Methods: Rats with collagen-induced arthritis (CIA) were treated with T-614 (5 and 20 mg/kg) daily. Animals receiving methotrexate (1 mg/kg every 3 days) and the nonsteroidal anti-inflammatory agent nimesulide (10 mg/kg per day) were used as controls. A combination therapy group was treated with both T-614(10 mg/kg per day) and methotrexate (1 mg/kg every 3 days). Hind paw swelling was evaluated and radiographic scores calculated. Serum cytokine levels were assessed by Bio-plex analysis. Quantitative PCR was used to evaluate expression of mRNA for interferon-gamma, IL-4 and IL-17. Serum IL-17 and anti-type II collagen antibodies (total IgG, IgG1, IgG2a, IgG2b and IgM) were measured using ELISA., Results: Oral T-614 inhibited paw swelling and offered significant protection against arthritis-induced cartilage and bone erosion, comparable to the effects of methotrexate. CIA rats treated with T-614 exhibited decreases in both mRNA expression of IL-17 in peripheral blood mononuclear cells and lymph node cells, and circulating IL-17 in a dose-dependent manner. T-614 also reduced serum levels of tumor necrosis factor-alpha, IL-1beta and IL-6. A synergistic effect was observed for the combination of methotrexate and T-614. In addition, T-614 (20 mg/kg per day) depressed production of anti-type II collagen antibodies and differentially affected levels of IgG2a subclasses in vivo, whereas IgM level was decreased without any change in the IgG1 level. Together, the findings presented here indicate that the novel agent T-614 has disease-modifying effects against experimental arthritis, as opposed to nimesulide., Conclusions: Our data suggested that T-614 is an effective disease-modifying agent that can prevent bone/cartilage destruction and inflammation in in CIA rats. Combination with methotrexate markedly enhances the therapeutic effect of T-614.
- Published
- 2008
- Full Text
- View/download PDF
32. Association of elevated transcript levels of interferon-inducible chemokines with disease activity and organ damage in systemic lupus erythematosus patients.
- Author
-
Fu Q, Chen X, Cui H, Guo Y, Chen J, Shen N, and Bao C
- Subjects
- Adolescent, Adult, Female, Humans, Leukocytes, Male, Middle Aged, Reverse Transcriptase Polymerase Chain Reaction, Biomarkers analysis, Chemokines biosynthesis, Interferons metabolism, Lupus Erythematosus, Systemic metabolism, Lupus Erythematosus, Systemic pathology, Lupus Erythematosus, Systemic physiopathology
- Abstract
Introduction: Systemic lupus erythematosus (SLE) is a multi-system autoimmune disease with a heterogeneous course and varying degrees of severity and organ damage; thus, there is increasing interest in identifying biomarkers for SLE. In this study we correlated the combined expression level of multiple interferon-inducible chemokines with disease activity, degree of organ damage and clinical features in SLE, and we investigated their roles as biomarkers., Methods: Peripheral blood cells obtained from 67 patients with SLE patients, 20 patients with rheumatoid arthritis (RA) and 23 healthy donors were subjected to real-time PCR in order to measure the transcriptional levels of seven interferon-inducible chemokines (RANTES, MCP-1, CCL19, MIG, IP-10, CXCL11, and IL-8). The data were used to calculate a chemokine score for each participant, after which comparisons were performed between various groups of SLE patients and control individuals., Results: Chemokine scores were significantly elevated in SLE patients versus RA patients and healthy donors (P = 0.012 and P = 0.002, respectively). Chemokine scores were correlated positively with SLE Disease Activity Index 2000 scores (P = 0.005) and negatively with C3 levels (P < 0.001). Compared with patients without lupus nephritis and those with inactive lupus nephritis, chemokine scores were elevated in patients with active lupus nephritis, especially when their daily prednisone dosage was under 30 mg (P = 0.002 and P = 0.014, respectively). Elevated chemokine scores were also associated with the presence of cumulative organ damage (Systemic Lupus International Collaborating Clinics/American Society of Rheumatology Damage Index >or= 1; P = 0.010) and the occurrence of anti-Sm or anti-RNP autoantibodies (both P = 0.021)., Conclusions: The combined transcription level of interferon-inducible chemokines in peripheral blood leucocytes is closely associated with disease activity, degree of organ damage, and specific autoantibody patterns in SLE. The chemokine score may serve as a new biomarker for active and severe disease in SLE.
- Published
- 2008
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.