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Your search keyword '"Sacha Inchi"' showing total 7 results
Start Over Descriptor "Sacha Inchi" Publisher biomed central
7 results on '"Sacha Inchi"'

1. <italic>De novo</italic> transcriptome assembly of the eight major organs of Sacha Inchi (<italic>Plukenetia volubilis</italic>) and the identification of genes involved in α-linolenic acid metabolism.

Author

Hu, Xiao-Di, Pan, Bang-Zhen, Fu, Qiantang, Niu, Longjian, Chen, Mao-Sheng, and Xu, Zeng-Fu

Subjects
*SACHA inchi, *MOLECULAR genetics, *ISOELECTRIC proteins, *GENE expression, *PLANT genetics
Abstract

Background: Sacha Inchi (Plukenetia volubilis L.), which belongs to the Euphorbiaceae, has been considered a new potential oil crop because of its high content of polyunsaturated fatty acids in its seed oil. The seed oil especially contains high amounts of α-linolenic acid (ALA), which is useful for the prevention of various diseases. However, little is known about the genetic information and genome sequence of Sacha Inchi, which has largely hindered functional genomics and molecular breeding studies. Results: In this study, a de novo transcriptome assembly based on transcripts sequenced in eight major organs, including roots, stems, shoot apexes, mature leaves, male flowers, female flowers, fruits, and seeds of Sacha Inchi was performed, resulting in a set of 124,750 non-redundant putative transcripts having an average length of 851 bp and an N50 value of 1909 bp. Organ-specific unigenes analysis revealed that the most organ-specific transcripts are found in female flowers (2244 unigenes), whereas a relatively small amount of unigenes are detected to be expressed specifically in other organs with the least in stems (24 unigenes). A total of 42,987 simple sequence repeats (SSRs) were detected, which will contribute to the marker assisted selection breeding of Sacha Inchi. We analyzed expression of genes related to the α-linolenic acid metabolism based on the de novo assembly and annotation transcriptome in Sacha Inchi. It appears that Sacha Inchi accumulates high level of ALA in seeds by strong expression of biosynthesis-related genes and weak expression of degradation-related genes. In particular, the up-regulation of FAD3 and FAD7 is consistent with high level of ALA in seeds of Sacha Inchi compared with in other organs. Meanwhile, several transcription factors (ABI3, LEC1 and FUS3) may regulate key genes involved in oil accumulation in seeds of Sacha Inchi. Conclusions: The transcriptome of major organs of Sacha Inchi has been sequenced and de novo assembled, which will expand the genetic information for functional genomic studies of Sacha Inchi. In addition, the identification of candidate genes involved in ALA metabolism will provide useful resources for the genetic improvement of Sacha Inchi and the metabolic engineering of ALA biosynthesis in other plants. [ABSTRACT FROM AUTHOR]

Published
2018
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3. Transcriptome analysis of Sacha Inchi (Plukenetia volubilis L.) seeds at two developmental stages.

Author

Xiaojuan Wang, Ronghua Xu, Ruling Wang, and Aizhong Liu

Subjects
*SACHA inchi, *OILSEED plants, *UNSATURATED fatty acids, *LINOLENIC acids, *BIOSYNTHESIS, *TRIGLYCERIDES, *GENE expression, *DESATURASES
Abstract

Background: Sacha Inchi (Plukenetia volubilis L., Euphorbiaceae) is a potential oilseed crop because the seeds of this plant are rich in unsaturated fatty acids (FAs). In particular, the fatty acid composition of its seed oil differs markedly in containing large quantities of a-linolenic acid (18C:3, a kind of ?-3 FAs). However, little is known about the molecular mechanisms responsible for biosynthesis of unsaturated fatty acids in the developing seeds of this species. Transcriptome data are needed to better understand these mechanisms. Results: In this study, de novo transcriptome assembly and gene expression analysis were performed using Illumina sequencing technology. A total of 52.6 million 90-bp paired-end reads were generated from two libraries constructed at the initial stage and fast oil accumulation stage of seed development. These reads were assembled into 70,392 unigenes; 22,179 unigenes showed a 2-fold or greater expression difference between the two libraries. Using this data we identified unigenes that may be involved in de novo FA and triacylglycerol biosynthesis. In particular, a number of unigenes encoding desaturase for formation of unsaturated fatty acids with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified. Conclusions: This study provides the first comprehensive dataset characterizing Sacha Inchi gene expression at the transcriptional level. These data provide the foundation for further studies on molecular mechanisms underlying oil accumulation and PUFA biosynthesis in Sacha Inchi seeds. Our analyses facilitate understanding of the molecular mechanisms responsible for the high unsaturated fatty acids (especially a-linolenic acid) accumulation in Sacha Inchi seeds. [ABSTRACT FROM AUTHOR]

Published
2012
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6. De novo transcriptome assembly of the eight major organs of Sacha Inchi (Plukenetia volubilis) and the identification of genes involved in α-linolenic acid metabolism

Author

Mao-Sheng Chen, Zeng-Fu Xu, Longjian Niu, Qiantang Fu, Bang-Zhen Pan, and Xiao-Di Hu

Subjects
0106 biological sciences, 0301 basic medicine, lcsh:QH426-470, lcsh:Biotechnology, De novo transcriptome assembly, α-linolenic acid metabolism, Biology, Genes, Plant, 01 natural sciences, Genome, Transcriptome, de novo transcriptome, 03 medical and health sciences, Sacha Inchi, lcsh:TP248.13-248.65, Plukenetia volubilis, Genetics, Gene, Gene Expression Profiling, Euphorbiaceae, food and beverages, alpha-Linolenic Acid, Molecular Sequence Annotation, Marker-assisted selection, biology.organism_classification, lcsh:Genetics, 030104 developmental biology, Genetic marker, Organ-specific gene expression, Functional genomics, 010606 plant biology & botany, Biotechnology, Research Article, Microsatellite Repeats
Abstract

Background Sacha Inchi (Plukenetia volubilis L.), which belongs to the Euphorbiaceae, has been considered a new potential oil crop because of its high content of polyunsaturated fatty acids in its seed oil. The seed oil especially contains high amounts of α-linolenic acid (ALA), which is useful for the prevention of various diseases. However, little is known about the genetic information and genome sequence of Sacha Inchi, which has largely hindered functional genomics and molecular breeding studies. Results In this study, a de novo transcriptome assembly based on transcripts sequenced in eight major organs, including roots, stems, shoot apexes, mature leaves, male flowers, female flowers, fruits, and seeds of Sacha Inchi was performed, resulting in a set of 124,750 non-redundant putative transcripts having an average length of 851 bp and an N50 value of 1909 bp. Organ-specific unigenes analysis revealed that the most organ-specific transcripts are found in female flowers (2244 unigenes), whereas a relatively small amount of unigenes are detected to be expressed specifically in other organs with the least in stems (24 unigenes). A total of 42,987 simple sequence repeats (SSRs) were detected, which will contribute to the marker assisted selection breeding of Sacha Inchi. We analyzed expression of genes related to the α-linolenic acid metabolism based on the de novo assembly and annotation transcriptome in Sacha Inchi. It appears that Sacha Inchi accumulates high level of ALA in seeds by strong expression of biosynthesis-related genes and weak expression of degradation-related genes. In particular, the up-regulation of FAD3 and FAD7 is consistent with high level of ALA in seeds of Sacha Inchi compared with in other organs. Meanwhile, several transcription factors (ABI3, LEC1 and FUS3) may regulate key genes involved in oil accumulation in seeds of Sacha Inchi. Conclusions The transcriptome of major organs of Sacha Inchi has been sequenced and de novo assembled, which will expand the genetic information for functional genomic studies of Sacha Inchi. In addition, the identification of candidate genes involved in ALA metabolism will provide useful resources for the genetic improvement of Sacha Inchi and the metabolic engineering of ALA biosynthesis in other plants. Electronic supplementary material The online version of this article (10.1186/s12864-018-4774-y) contains supplementary material, which is available to authorized users.

Published
2018

7. Apparent digestibility of dry matter, organic matter, protein and energy of native Peruvian feedstuffs in juvenile rainbow trout (Oncorhynchus mykiss)

Author

Abimael Ortiz-Chura, Marcelino Jorge Araníbar Araníbar, Francisco Halley Rodriguez Huanca, Ruth Milagro Pari-Puma, and María Esperanza Cerón-Cucchi

Subjects
0301 basic medicine, Amaranthus Caudatus, Oncorhynchus Mykiss, Aquatic Science, Materia Orgánica, Oceanography, Biochemistry, Chenopodium quinoa, lcsh:Aquaculture. Fisheries. Angling, Rainbow trout feed, 03 medical and health sciences, Perú, Animal science, Plukenetia volubilis, Dry matter, Digestibilidad, Molecular Biology, lcsh:SH1-691, Meal, biology, Chenopodium Quinoa, Proteins, Jumbo squid meal, 04 agricultural and veterinary sciences, biology.organism_classification, Trout, 030104 developmental biology, Animal ecology, Organic Matter, Trucha Arco Iris, Chenopodium Pallidicaule, Digestibility, Quinoa, Proteínas, Sacha inchi, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Rainbow trout, Chenopodium pallidicaule, Traditional Foods, Alimentos Tradicionales
Abstract

Trout production is a growing activity in recent years but requires new alternative sources of feed to be sustainable over time. The objective of this research was to determine the apparent digestibility coefficient (ADC) of dry matter (DM), organic matter (OM), crude protein (CP) and digestible energy (DE) of kañiwa (Chenopodium pallidicaule Aellen), kiwicha (Amaranthus caudatus L), quinoa (Chenopodium quinoa Willd), beans (Phaseolus vulgaris L.), sacha inchi, (Plukenetia volubilis L) and jumbo squid (Dosidicus gigas) meal in juvenile rainbow trout. The experimental diets were composed of a 70% basal diet and 30% of any raw materials. The ADC was determined by the indirect method using insoluble ash as a non-digestible marker. Jumbo squid, sacha inchi and quinoa showed the highest values of ADC (%) of DM (84.5, 73.5 and 69.7), OM (89.1, 78.4 and 72.9), CP (93.2, 98.0 and 90.3), and DE (4.57, 4.15 and 2.95 Mcal/kg DM), respectively. The ADC values for kañiwa, kiwicha and bean were significantly lower. In conclusion, quinoa meal and jumbo squid meal have an acceptable digestibility but sacha inchi meal is a potential alternative for rainbow trout feeding in the future. Instituto de Patobiología Fil: Ortiz Chura, Abimael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pari Puma, Ruth Milagro. Universidad Nacional del Altiplano. Facultad de Medicina Veterinaria y Zootecnia; Perú Fil: Rodríguez Huanca, Francisco Halley. Universidad Nacional del Altiplano. Facultad de Medicina Veterinaria y Zootecnia; Perú Fil: Ceron Cucchi, Maria Esperanza. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina Fil: Araníbar Araníbar, Marcelino Jorge. Universidad Nacional del Altiplano. Facultad de Medicina Veterinaria y Zootecnia; Perú

Published
2018
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