1. Surveillance of pneumococcal serotype 1 carriage during an outbreak of serotype 1 invasive pneumococcal disease in central Australia 2010–2012
- Author
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Jana Yr Lai, Heather Cook, Teem-Wing Yip, Jeanette Berthelsen, Helen V. Smith, Amanda J. Leach, Stephen Gourley, Vicki Krause, and Heidi C. Smith-Vaughan
- Subjects
Serotype ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,Central Australia ,medicine.disease_cause ,Pneumococcal Infections ,Disease Outbreaks ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Medical microbiology ,Nasopharynx ,Streptococcus pneumoniae ,medicine ,Humans ,030212 general & internal medicine ,Typing ,Child ,Aged ,0303 health sciences ,Carriage ,030306 microbiology ,business.industry ,Australia ,Outbreak ,Infant ,Invasive pneumococcal disease ,Middle Aged ,medicine.disease ,Virology ,3. Good health ,Pneumococcal infections ,Infectious Diseases ,Child, Preschool ,Carrier State ,Epidemiological Monitoring ,Serotype 1 ,Multilocus sequence typing ,Female ,business ,Research Article - Abstract
Background An outbreak of serotype 1 invasive pneumococcal disease (IPD) occurred in Central Australia from October 2010 to the latter part of 2012. Surveillance of serotype 1 carriage was conducted to determine epidemiological features of asymptomatic carriage that could potentially be driving the outbreak. Methods 130 patients and accompanying persons presenting at Alice Springs Hospital Emergency Department consented to nasopharyngeal swab (NPS) collection. NPS were processed by standard methods, including culture, pneumococcal lytA quantitative real-time PCR, serotype 1-specific real-time PCR and multi-locus sequence typing (MLST). Results Pneumococcal carriage was detected in 16% of participants. Carriage was highest in the under 10 year olds from remote communities surrounding Alice Springs (75%). Four NPS were positive for serotype 1 DNA by PCR; 3 were also culture-positive for serotype 1 pneumococci. Serotype 1 isolates had atypical colony morphology on primary culture. All serotype 1 carriers were healthy children 5 to 8 years of age from remote communities. By MLST, serotype 1 isolates were ST306, as were IPD isolates associated with this outbreak. Conclusions During an outbreak of serotype 1 ST306 IPD, carriage of the outbreak strain was detected in 3% NPS collected. All carriers were healthy children 5 to 8 years of age.
- Published
- 2013