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Start Over Author "Ruifang Wu" Publisher biomed central
3 results on '"Ruifang Wu"'

2. Roles of extended human papillomavirus genotyping and multiple infections in early detection of cervical precancer and cancer and HPV vaccination

Author

Fangbin Song, Peisha Yan, Xia Huang, Chun Wang, Hui Du, Xinfeng Qu, and Ruifang Wu

Subjects
Adult, Cancer Research, Human papillomavirus, Genotype, Genotyping Techniques, Uterine Cervical Neoplasms, Predictive Value of Tests, Genetics, Humans, Papillomavirus Vaccines, Papillomaviridae, RC254-282, Early Detection of Cancer, Cervical intraepithelial neoplasia, Research, Incidence, Vaccination, Papillomavirus Infections, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Middle Aged, Uterine Cervical Dysplasia, female genital diseases and pregnancy complications, Logistic Models, Oncology, Cervical cancer, Screening, Female, Precancerous Conditions
Abstract

Background The aim of the study was to investigate the risk of human papillomavirus (HPV) genotyping particularly vaccine genotypes and multiple infections for cervical precancer and cancer, which might contribute to developing genotype-specific screening strategy and assessing potential effects of HPV vaccine. Methods The HPV genotypes were identified using the Seq HPV assay on self-collected samples. Hierarchical ranking of each genotype was performed according to positive predictive value (PPV) for cervical intraepithelial neoplasia 2/3 or worse (CIN2+/CIN3+). Multivariate logistic regression model was used to estimate the odds ratios (ORs) with 95% confidence interval (CI) of CIN2+ according to multiplicity of types and vaccine types. Results A total of 2811 HPV-positive women were analyzed. The five dominant HPV genotypes in high-grade lesions were 16/58/52/33/18. The overall ranking orders were HPV16/33/35/58/31/68/18/ 56/52/66/51/59/45/39 for CIN2+ and HPV16/33/31/58/45/66/52/18/35/56/51/68/59/39 for CIN3+. The risks of single infection versus co-infections with other types lower in the hierarchy having CIN2+ were not statistically significant for HPV16 (multiple infection vs. single infection: OR = 0.8, 95%CI = 0.6-1.1, P = 0.144) or other genotypes (P > 0.0036) after conservative Bonferroni correction. Whether HPV16 was present or not, the risks of single infection versus multiple infection with any number (2, ≥2, or ≥ 3) of types for CIN2+ were not significantly different. In addition, HPV31/33/45/52/58 covered by nonavalent vaccine added 27.5% of CIN2, 23.0% of CIN3, and 12.5% of cancer to the HPV16/18 genotyping. These genotype-groups were at significantly higher risks than genotypes not covered by nonavalent vaccine. Moreover, genotypes covered by nonavalent vaccine contributed to 85.2% of CIN2 lesions, 97.9% of CIN3 and 93.8% of cancers. Conclusions Partial extended genotyping such as HPV33/31/58 but not multiplicity of HPV infections could serve as a promising triage for HPV-positive self-samples. Moreover, incidence rates of cervical cancer and precancer were substantial attributable to HPV genotypes covered by current nonavalent vaccination.

Published
2022

3. Amplification and up-regulation of MIR30D was associated with disease progression of cervical squamous cell carcinomas.

Author

You Zhou, Yinghua Hao, Yuxia Li, Ruizhen Li, Ruifang Wu, Shubin Wang, Zhengyu Fang, Zhou, You, Hao, Yinghua, Li, Yuxia, Li, Ruizhen, Wu, Ruifang, Wang, Shubin, and Fang, Zhengyu

Subjects
DISEASE progression, SQUAMOUS cell carcinoma, OVARIAN epithelial cancer, MICRORNA, CELL proliferation, RNA metabolism, ANIMAL experimentation, BIOCHEMISTRY, CELL lines, GENES, GENETICS, PHENOMENOLOGY, MICE, RNA, CERVIX uteri tumors
Abstract

Background: Cervical squamous cell carcinoma (CSCC) is the most frequent type among cervical cancers. Although the altered miRNA miR-30d expression and the amplified chromosome locus of MIR30D, 8q24, have been reported in somatic cancers, the definitive functional impact of such region especially in CSCC remains under-investigated.Methods: One hundred thirty-six cases of CSCC tissues and matched adjacent normal ovarian epithelial tissues were assessed in this study. FISH and qPCR were performed to detect the copy number and microRNA expression of MIR30D gene in the collected samples. In in-vitro study, proliferation of CSCC cells were analyzed using WST-1 assay and invasion abilities of CSCC cells were evaluated by transwell assay. In-vivo study using a model of nude mice bearing tumor was also performed.Results: Copy number gains of MIR30D were detected in 22.8% (31 out of 136) of CSCC samples. Copy number of MIR30D was positively correlated with tumor progression. CSCCs with lymph node metastases (LNM) also showed more frequencies (36.4%) of MIR30D amplification than those without LNM (18.4%, p < 0.05). CSCCs with increased copy number of MIR30D also showed a positive correlation with miR-30d up-regulation. Inhibition of miR-30d in CSCC cells led to impaired tumor growth and migration.Conclusions: Copy number amplifications of MIR30D gene and enhanced expression of miR-30d were positively correlated with tumor progression in CSCCs, indicating miR-30d might play an oncomiric role in the progression of CSCC. [ABSTRACT FROM AUTHOR]

Published
2017
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