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The aim of the study was to represent chronic stress exposure by a complex generic Active and Healthy (AHA) diagnostic assessment tool incorporating ICF. This is a single-centre, cross-sectional, observational, non-interventional, non-randomized trial in University based women's hospital, division of Gynecological Endocrinology and Reproductive Medicine. All participants followed a standardized, holistic battery of biopsychosocial assessments consisting of bio-functional status (BFS), bio-functional age (BFA) and the questionnaire for chronic stress exposure (TICS). 624 non-pediatric, non-geriatric subjects were recruited in the BeCS-14 cohort. The mean difference between chronological age and BFA was 7.8 ± 8.0 year equivalents. The mean stress level score assessed by SSCS was 13.2 with 45.4% being exposed to above average stress intensity. 22 BFS items (14 objective, 7 subjective) significantly correlated with chronic stress exposure (TICS-SSCS). The constructed sum score composed of SOC&#95;L9 and complaint questionnaire (physical and emotional wellbeing) represented chronic stress exposure best (pearson-correlation value 0.564, p  < 0.0001). Higher chronic stress exposure was associated with bio-functional pro-aging (less vitality) in both sexes. In conclusion, chronic stress is accepted as a major risk factor for developing non-communicable diseases (NCD). Our ICF compatible, complex, generic BFS/BFA assessment tool reflects chronic stress exposure and may be applied in various health care settings, e.g., in health promotion and prevention of NCDs., Competing Interests: The study protocol was approved by the Cantonal Ethics Committee Bern (Ref.-Nr. KEK-BE: 023112), and written informed consent was obtained from each participant.All authors agree to publication and adhere to the journal’s requirements.P. Stute, M. von Wolff, N. Bitterlich, L. Hollenstein and M. Anker declare to have no conflict of interest in context of this manuscript. F. Meissner is managing director of vital.services and D. Poethig is member of the scientific board of vital.services, which provides the technology for BFS measuring. F. Meissner has no involvements that might raise the question of bias in the work reported or in the conclusions, implications, or opinions stated.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.