16 results on '"Nan Yu"'
Search Results
2. Prognostic value of endothelial biomarkers in refractory cardiogenic shock with ECLS: a prospective monocentric study
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Tsai, Tsung-Yu, Tu, Kun-Hua, Tsai, Feng-Chun, Nan, Yu-Yun, Fan, Pei-Chun, Chang, Chih-Hsiang, Tian, Ya-Chung, Fang, Ji-Tseng, Yang, Chih-Wei, and Chen, Yung-Chang
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- 2019
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3. Detection and characterization of human astrovirus and sapovirus in outpatients with acute gastroenteritis in Guangzhou, China
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Jian-Kai Deng, Xin Luo, Xiao-Yan Che, Nan Yu, and Xiao-Ping Mu
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Genotype ,RC799-869 ,medicine.disease_cause ,Sapovirus ,law.invention ,Astrovirus ,Feces ,law ,Rotavirus ,Outpatients ,Medicine ,Humans ,Acute gastroenteritis ,Polymerase chain reaction ,Phylogeny ,biology ,Molecular epidemiology ,business.industry ,Research ,Gastroenterology ,Infant ,Clinical features ,General Medicine ,Diseases of the digestive system. Gastroenterology ,biology.organism_classification ,medicine.disease ,Virology ,Gastroenteritis ,Norovirus ,Coinfection ,business ,Mamastrovirus - Abstract
Background Human astrovirus (HAstV) and sapovirus (SaV) are common pathogens that can cause acute gastroenteritis (AGE). However, very few studies have reported the molecular epidemiology and clinical information on HAstV and SaV in China. This study aims to determine the molecular epidemiology and clinical features of HAstV and SaV in patients with AGE in Guangzhou, China. Methods For this study, 656 patients with AGE were enrolled. Their stool samples were screened for 15 enteropathogens using Luminex xTAG® Gastrointestinal Pathogen Panel. HAstV and SaV were detected through an in-house multiplex reverse transcriptase polymerase chain reaction followed by phylogenetic analysis. We described and compared clinical features of AGE in patients with HAstV and SaV. Results Of the 656 stool samples, 63.72% (418/656) were found to be positive, with 550 enteropathogens (296 bacteria and 254 viruses). HAstV and SaV were detected in 20 (3.0%) and 12 (1.8%) samples, respectively. Four genotypes (genotypes 1, 2, 3, and 8) of HAstV and three genotypes (GI.1, GI.2 and GIV) of SaV were identified. Coinfection was observed in ten HAstV-positive and two SaV-positive samples. HAstV was more likely to occur in winter, while SaV in early spring. The median age of the patients with single HAstV infection was higher than that of the patients with other viruses (rotavirus, norovirus, and enteric adenovirus; P = 0.0476) and unknown etiology (P = 0.006). Coinfection with HAstV or SaV were not associated with disease severity (P > 0.05). Conclusion HAstV and SaV are the common causes of AGE in Guangzhou, China.
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- 2021
4. Prognostic value of clinical and morphologic findings in patients with type B aortic intramural hematoma
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Jian Zhang, Nan Yu, Guangqi Chang, Jinghong Tan, Chen Yao, Hong Pan, Zilun Li, Ridong Wu, Zhuang Guo, Yingying Guo, and Chenshu Liu
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Male ,genetic structures ,Kaplan-Meier Estimate ,030204 cardiovascular system & hematology ,Gastroenterology ,030218 nuclear medicine & medical imaging ,0302 clinical medicine ,Risk Factors ,Medicine ,Aorta ,Acute aortic syndrome ,Aortic dissection ,Hematoma ,General Medicine ,Organ Size ,Middle Aged ,Prognosis ,Cardiac surgery ,Survival Rate ,Cardiothoracic surgery ,Disease Progression ,Female ,Chinese population ,Cardiology and Cardiovascular Medicine ,Research Article ,Pulmonary and Respiratory Medicine ,Adult ,medicine.medical_specialty ,lcsh:Surgery ,Aortic Diseases ,lcsh:RD78.3-87.3 ,03 medical and health sciences ,Internal medicine ,medicine.artery ,Humans ,Risk factor ,Survival analysis ,Ulcer ,Aged ,Proportional Hazards Models ,Retrospective Studies ,business.industry ,Proportional hazards model ,lcsh:RD1-811 ,medicine.disease ,Atherosclerosis ,Aortic Dissection ,lcsh:Anesthesiology ,Surgery ,Aortic intramural hematoma ,business ,Penetrating atherosclerosis ulcer - Abstract
Background Aortic intramural hematoma (IMH) is a subset of acute aortic syndrome, and its prognosis may differ between races. This study aimed to study the prognosis of Chinese type B IMH patients and to find out risk factors. Methods A total of 71 type B IMH patients with or without penetrating atherosclerosis ulcer (PAU) administrated in our center between September 2013 and October 2017 were retrospectively studied. Both clinical and imaging data were collected and analyzed. The primary end point was aorta-related death, and the secondary end point was progression, which was defined as enlargement of aorta, increased aortic wall thickness, and aortic dissection or aneurysm formation. Kaplan-Meier survival analysis and Cox regression analysis were used for prognostic analysis. Results Among these 71 patients, 21 had simple type B IMH, when 50 had type B IMH in association with PAU. Twenty-five patients received optimal medical therapy (OMT) alone, while 46 patients received surgery and OMT. The mean follow-up time was 27.5 ± 13.5 months. For type B IMH patients, association with PAU indicated poor prognosis and required more intensive management (HR = 16.68, 1.96~141.87), while maximum aortic diameter (MAD) was an independent risk factor (HR = 1.096, 1.016~1.182). For patients with PAU-IMH, MAD was an independent risk factor (HR = 1.04, 1.021~1.194), while surgical treatment was independent protective factor (HR = 0.172, 0.042~0.696). Conclusion Association with PAU and MAD were independent risk factors for type B IMH patients. Surgery may improve the outcomes for type B IMH in association with PAU.
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- 2020
5. Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2
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Dian-Hui Xiu, Gui-Feng Liu, Shao-Nan Yu, Long-Yun Li, Guo-Qing Zhao, Lin Liu, and Xue-Feng Li
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LINC00968 ,Wnt2/β-catenin signaling pathway ,Breast cancer ,WNT2 ,Research ,Drug resistance ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,lcsh:RC254-282 ,HEY1 - Abstract
Background Breast cancer is one the most common cancers, making it the second leading cause of cancer-related death among women. Long non-coding RNAs (lncRNAs), with tightly regulated expression patterns, also serve as tumor suppressor during tumorigenesis. The present study aimed to elucidate the role of LINC00968 in breast cancer via WNT2-mediated Wnt2/β-catenin signaling pathway. Methods Breast cancer chip GSE26910 was utilized to identify differential expression in LINC00968 and WNT2. The possible relationship among LINC00968, transcriptional repressor HEY and WNT2 was analyzed and then verified. Effects of LINC00968 on activation of the Wnt2/β-catenin signaling pathway was also tested. Drug resistance, colony formation, cell migration, invasion ability and cell apoptosis after transfection were also determined. Furthermore, tumor xenograft in nude mice was performed to test tumor growth and weight in vivo. Results WNT2 expression exhibited at a high level, whereas LINC00968 at a low expression in breast cancer which was also associated with poor prognosis in patients. LINC00968 targeted and negatively regulated WNT2 potentially via HEY1. Either overexpressed LINC00968 or silenced inhibited activation of the Wnt2/β-catenin signaling pathway, thereby reducing drug resistance, decreasing colony formation ability, as well as suppressing migration and invasion abilities of breast cancer cells in addition to inducing apoptosis. Lastly, in vivo experiment suggested that LINC00968 overexpression also suppressed transplanted tumor growth in nude mice. Conclusion Collectively, overexpressed LINC00968 contributes to reduced drug resistance in breast cancer cells by inhibiting the activation of the Wnt2/β-catenin signaling pathway through silencing WNT2. This study offers a new target for the development of breast cancer treatment.
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- 2019
6. Clinical characteristics of acute hepatitis A outbreak in Taiwan, 2015-2016: observations from a tertiary medical center.
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Nan-Yu Chen, Zhuo-Hao Liu, Shian-Sen Shie, Tsung-Hsing Chen, Ting-Shu Wu, Chen, Nan-Yu, Liu, Zhuo-Hao, Shie, Shian-Sen, Chen, Tsung-Hsing, and Wu, Ting-Shu
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HEPATITIS A , *MEN who have sex with men , *HIV infections , *HIV infection transmission , *SEXUALLY transmitted diseases , *SYPHILIS , *BILIRUBIN , *SYPHILIS epidemiology , *EPIDEMIOLOGY of sexually transmitted diseases , *HIV infection epidemiology , *DISEASE outbreaks , *HOMOSEXUALITY , *COMORBIDITY , *AIDS-related opportunistic infections , *RETROSPECTIVE studies , *MIXED infections - Abstract
Background: Acute hepatitis A is a fecal-oral transmitted disease related to inadequate sanitary conditions. In addition to its traditional classification, several outbreaks in the men who have sex with men (MSM) population have resulted in acute hepatitis A being recognized as a sexually transmitted disease. However, few studies have clarified the clinical manifestations in these outbreaks involving the MSM population.Methods: Beginning in June 2015, there was an outbreak of acute hepatitis A involving the MSM population in Northern Taiwan. We conducted a 15-year retrospective study by recruiting 207 patients with the diagnosis of acute hepatitis A that included the pre-outbreak (January 2001 to May 2015) and outbreak (June 2015 to August 2016) periods in a tertiary medical center in Northern Taiwan. Using risk factors, comorbidities, presenting symptoms, laboratory test results and imaging data, we aimed to evaluate the clinical significance of acute hepatitis A in the MSM population, where human immunodeficiency virus (HIV) coinfection is common.Results: There was a higher prevalence of reported MSM (p < 0.001), HIV (p < 0.001) and recent syphilis (p < 0.05) coinfection with acute hepatitis A during the outbreak period. The outbreak population had more prominent systemic symptoms, was more icteric with a higher total bilirubin level (p < 0.05) and had a 7-times higher tendency (p < 0.05) to have a hepatitis A relapse.Conclusions: The clinical course of acute hepatitis A during an outbreak involving the MSM and HIV-positive population is more symptomatic and protracted than in the general population. [ABSTRACT FROM AUTHOR]- Published
- 2017
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7. Metabolic syndrome is independently associated with a mildly reduced estimated glomerular filtration rate: a cross-sectional study.
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Wen Hu, Xiao-Juan Wu, Yao-Jun Ni, Hai-Rong Hao, Wei-Nan Yu, Hong-Wen Zhou, Hu, Wen, Wu, Xiao-Juan, Ni, Yao-Jun, Hao, Hai-Rong, Yu, Wei-Nan, and Zhou, Hong-Wen
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METABOLIC syndrome ,GLOMERULAR filtration rate ,KIDNEY diseases ,CREATININE ,PUBLIC health - Abstract
Background: Association between metabolic syndrome (MS) and mildly reduced estimated glomerular filtration rates (eGFRs) remains unclear. Therefore, we aimed to evaluate the association between MS and a mildly reduced eGFR in Chinese adults.Methods: Anthropometric and biochemical examinations were performed in 2992 individuals. The eGFR was calculated from the creatinine level. MS was defined according to the Adult Treatment Panel III criteria as the presence of three or more risk factors. Mildly reduced eGFR was defined as a value between 60 and 90 mL/min/1.73 m2. Multiple linear regression and multiple logistic regression analysis were used to evaluate association between metabolic syndrome and estimate glomerular filtration rate.Results: After adjusting for several potential confounders, the participants with MS showed a 1.29-fold increased odds ratio for a mildly reduced eGFR compared with those without MS. Additionally, the odds ratios (and 95% confidence intervals (CIs)) for mildly reduced eGFR in participants with elevated triglycerides (TG), decreased high-density lipoprotein (HDL), obesity and elevated fasting blood glucose (FPG) after multivariable adjustment were 1.25 (1.05-1.49), 1.23 (1.03-1.48), 1.22 (1.03-1.45) and 0.64 (0.52-0.78), respectively. The odds ratios (95% CIs) for hyperfiltration in participants with elevated FPG and HbA1c levels after multivariable adjustment were 1.53 (1.30-1.81) and 2.86 (2.00-4.09), respectively.Conclusions: MS is associated with an increased risk of a mildly reduced eGFR in the Chinese population, and several individual components of MS have different impacts on eGFR levels. MS had dual roles on renal damage.Trial Registration: ChiCTR-TRC- 14005029 . Registered 28 July 2014. [ABSTRACT FROM AUTHOR]- Published
- 2017
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8. Changes in enterovirus serotype constituent ratios altered the clinical features of infected children in Guangdong Province, China, from 2010 to 2013.
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Hong-Tao Zhou, Yong-Hui Guo, Man-Jun Chen, Yu-Xian Pan, Lin Xue, Bin Wang, Shao-Hua Tao, Nan Yu, Zhou, Hong-Tao, Guo, Yong-Hui, Chen, Man-Jun, Pan, Yu-Xian, Xue, Lin, Wang, Bin, Tao, Shao-Hua, and Yu, Nan
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ENTEROVIRUS diseases ,HERPANGINA ,JUVENILE diseases ,PUBLIC health administration ,PUBLIC health ,INFECTIOUS disease transmission ,COXSACKIEVIRUS diseases ,ECHO viruses ,ENTEROVIRUSES ,HOSPITAL care ,RETROSPECTIVE studies ,SEROTYPES ,HAND, foot & mouth disease - Abstract
Background: Enterovirus (EV)-related hand, foot, and mouth disease/herpangina (HFMD/HA) has been prevalent in Guangdong Province, China, since 2010.Methods: Clinical data for EV-related HFMD/HA inpatients admitted to the Department of Paediatrics of Zhujiang Hospital from 2010 to 2013 were retrospectively reviewed. The corresponding EV serotypes were also determined by reverse transcription-polymerase chain reaction or BLAST analysis of the sequenced partial lengths of the viral protein1/5'-untranslated region.Results: A total of 867 eligible inpatients admitted during 2010-2013 were included in the study. Of these, the serotype of the responsible EV was successfully identified in 824 cases. The incidence of enterovirus 71 (EV71) infection amongst pediatric HFMD/HA inpatients decreased dramatically from 55.5 % in 2010 to 8.1 % in 2013, with a similar decrease recorded for coxsackievirus A16 (CVA16). However, the incidence of non-EV71/CVA16 infection increased from 30.0 % in 2010 to 83.8 % in 2013. We noted that the types of infection caused by different EV serotypes varied: EV71 was responsible for 100 % of the paralysis cases (26/26), 84.6 % of the deaths (11/13), and 84.1 % of cases with severe central nervous system involvement (SCNSI) (74/88); echovirus contributed to 16.4 % of the deaths (2/13) and 4.4 % of the SCNSI cases; and coxsackievirus accounted for only 2.2 % of the SCNSI cases (2/90). The clinical features of HFMD/HA cases varied greatly during the time period examined, with drastic changes in the hospitalization rates (45.1, 63.7, 36.4, and 19.1 % for 2010, 2011, 2012, and 21013, respectively), mortality rates (2.3, 0.9, 2.5, and 0.0 %, respectively), paralysis (5.1, 1.2, 5.4, and 0.0 %, respectively), SCNSI (16.8, 7.1, 12.7, and 2.2 %, respectively), and acute respiratory infection (21.1, 22.0, 45.9, and 59.0 %, respectively).Conclusions: The incidences of infection caused by different EV serotypes, along with the clinical features of HFMD/HA cases, changed drastically in Guangdong Province, China, from 2010 to 2013, with the biggest changes observed in 2013. The changed constituent ratios of the different EV serotypes might therefore be responsible for the differences in the observed clinical features of HFMD/HA during this period. [ABSTRACT FROM AUTHOR]- Published
- 2016
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9. HIV-1 capsid is involved in post-nuclear entry steps.
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Nan-Yu Chen, Lihong Zhou, Gane, Paul J., Opp, Silvana, Ball, Neil J., Nicastro, Giuseppe, Zufferey, Madeleine, Buffone, Cindy, Luban, Jeremy, Selwood, David, Diaz-Griffero, Felipe, Taylor, Ian, and Fassati, Ariberto
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NUCLEOPORINS , *CAPSIDS , *NUCLEAR proteins , *ISOTHERMAL titration calorimetry , *MOLECULAR docking , *PROTEIN binding - Abstract
Background: HIV-1 capsid influences viral uncoating and nuclear import. Some capsid is detected in the nucleus but it is unclear if it has any function. We reported that the antibiotic Coumermycin-A1 (C-A1) inhibits HIV-1 integration and that a capsid mutation confers resistance to C-A1, suggesting that capsid might affect post-nuclear entry steps. Results: Here we report that C-A1 inhibits HIV-1 integration in a capsid-dependent way. Using molecular docking, we identify an extended binding pocket delimited by two adjacent capsid monomers where C-A1 is predicted to bind. Isothermal titration calorimetry confirmed that C-A1 binds to hexameric capsid. Cyclosporine washout assays in Jurkat CD4+ T cells expressing engineered human TRIMCyp showed that C-A1 causes faster and greater escape from TRIMCyp restriction. Sub-cellular fractionation showed that small amounts of capsid accumulated in the nuclei of infected cells and C-A1 reduced the nuclear capsid. A105S and N74D capsid mutant viruses did not accumulate capsid in the nucleus, irrespective of C-A1 treatment. Depletion of Nup153, a nucleoporin located at the nuclear side of the nuclear pore that binds to HIV-1 capsid, made the virus less susceptible to TRIMCyp restriction, suggesting that Nup153 may help maintain some integrity of the viral core in the nucleus. Furthermore C-A1 increased binding of CPSF6, a nuclear protein, to capsid. Conclusions: Our results indicate that capsid is involved in post-nuclear entry steps preceding integration. [ABSTRACT FROM AUTHOR]
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- 2016
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10. Enterovirus-related diarrhoea in Guangdong, China: clinical features and implications in hand, foot and mouth disease and herpangina.
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Hong-Tao Zhou, Hai-Su Yi, Yong-Hui Guo, Yu-Xian Pan, Shao-Hua Tao, Bin Wang, Man-Jun Chen, Mei Yang, Nan Yu, Zhou, Hong-Tao, Yi, Hai-Su, Guo, Yong-Hui, Pan, Yu-Xian, Tao, Shao-Hua, Wang, Bin, Chen, Man-Jun, Yang, Mei, and Yu, Nan
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DIARRHEA ,FOOT & mouth disease virus ,APHTHOVIRUSES ,CHILDREN ,PUBLIC health ,HEALTH ,COXSACKIEVIRUS diseases ,ENTEROVIRUS diseases ,ENTEROVIRUSES ,DISEASE prevalence ,HAND, foot & mouth disease ,GENOTYPES - Abstract
Background: A series of complications caused by enteroviruses, including meningitis, encephalitis, acute flaccid paralysis, acute cardiopulmonary failure, respiratory infection, and myocardial injury have been reported in hand, foot and mouth disease/herpangina (HFMD/HA). However, the complication of diarrhoea caused by enteroviruses has been neglected, and a summary of its clinical features and impact on HFMD/HA is unavailable.Methods: We included inpatients with HFMD/HA admitted to the Paediatric Department of Zhujiang Hospital during 2009-2012. We summarised and compared clinical data for cases with and without diarrhoea, and determined enterovirus serotypes by reverse transcriptase polymerase chain reaction and genotyping based on a partial-length fragment of viral protein 1 or the 5'-untranslated region.Results: There were 804 inpatients with HFMD/HA and 28 (3.5%) presented with diarrhoea. Gastrointestinal symptoms were mild in most cases of diarrhoea (82.1%), with high prevalence of no dehydration (82.1%), short duration of diarrhoea (78.6%) and watery stools (75.0%). The prevalence of multi-organ dysfunction syndrome (10.7 vs 0.40%) (p = 0.001), hepatic injury (14.3 vs 3.4%) (p = 0.019), myocardial injury (21.4 vs 6.1%) (p = 0.002) and convulsion (21.4 vs 7.2%) (p = 0.016) was significantly higher in the diarrhoea than no diarrhoea group. There was no significant difference between the two groups regarding prevalence of death, altered consciousness, paralysis, central nervous system involvement, or acute respiratory infection.Conclusions: Most patients with diarrhoea caused by enteroviruses circulating in Guangdong Province in 2009-2012 had mild or moderate gastrointestinal symptoms. Although enterovirus-related diarrhoea caused additional multi-organ dysfunction syndrome, hepatic injury and myocardial injury in children with HFMD/HA, timely intervention efficiently reduced disease severity and improved outcome. [ABSTRACT FROM AUTHOR]- Published
- 2016
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11. Danhong injection in the treatment of chronic stable angina: study protocol for a randomized controlled trial.
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Peng Qian Wang, Dan Dan Li, Wei Dong, Jun Liu, Ya Nan Yu, Chun Ti Shen, Qi Guang Chen, Bing Wei Chen, Yun Dai Chen, Zhong Wang, Wang, Peng Qian, Li, Dan Dan, Dong, Wei, Liu, Jun, Yu, Ya Nan, Shen, Chun Ti, Chen, Qi Guang, Chen, Bing Wei, Chen, Yun Dai, and Wang, Zhong
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ANGINA pectoris treatment ,CHINESE medicine ,CAUSES of death ,ALTERNATIVE medicine ,RESEARCH protocols ,RANDOMIZED controlled trials ,DRUG therapy for angina pectoris ,ANGINA pectoris ,CARDIOVASCULAR agents ,COMPARATIVE studies ,EXPERIMENTAL design ,HERBAL medicine ,INTRAVENOUS therapy ,LONGITUDINAL method ,RESEARCH methodology ,MEDICAL cooperation ,RESEARCH ,TIME ,EVALUATION research ,TREATMENT effectiveness ,BLIND experiment ,DRUG administration ,DRUG dosage ,DIAGNOSIS - Abstract
Background: Chronic stable angina is a leading cause of death worldwide. Danhong injection, a complementary alternative medicine for chronic stable angina, has been demonstrated to be effective in numerous studies and is widely prescribed to patients. However, the methodological quality of most prior studies was found to be, in general, low. Therefore, we designed this randomized controlled trial to evaluate the efficacy and safety of using Danhong injection to treat chronic stable angina.Methods/design: This is a randomized multicentre, double-blind, placebo-controlled, adaptive clinical trial. A total of 870 patients meeting the eligibility criteria will be randomly assigned into either the Danhong injection or the placebo group in a 2:1 ratio. Participants will then undergo a 2-week treatment regimen and a 76-day follow-up period. Because this is an adaptive trial, two interim analyses are prospectively planned. These will be performed after one-third and two-thirds of the patients, respectively, have completed the trial. Based on the results of these interim analyses, a data monitoring committee will determine how to modify aspects of the study without undermining the validity and integrity of the trial. The primary outcome measure is the proportion of patients who show a clinically significant change, which is defined as at least a 20-point improvement in angina frequency score on the Seattle Angina Questionnaire, which will be administered on day 30. Other secondary efficacy and safety outcomes will also be assessed.Discussion: This trial will provide high-quality evidence regarding the use of Danhong injection to treat chronic stable angina.Trial Registration: ClinicalTrials.gov: NCT01681316 . [ABSTRACT FROM AUTHOR]- Published
- 2015
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12. Time-dependent variation of pathways and networks in a 24-hour window after cerebral ischemia-reperfusion injury.
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Li-Ying Wang, Jun Liu, Yuan Li, Bing Li, Ying-Ying Zhang, Zhi-Wei Jing, Ya-Nan Yu, Hai-Xia Li, Shan-Shan Guo, Yi-Jun Zhao, Zhong Wang, and Yong-Yan Wang
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CEREBRAL ischemia ,REPERFUSION injury ,APOPTOSIS ,VASCULAR endothelial growth factors ,GENES - Abstract
Background: Cerebral ischemia-reperfusion injury may simultaneously result in functional variation of multiple genes/pathways. However, most prior time-sequence studies on its pathomechanism only focused on a single gene or pathway. Our study aimed to systematically analyze the time-dependent variation in the expression of multiple pathways and networks within 24 h after cerebral ischemia-reperfusion injury. Results: By uploading 374 ischemia-related genes into the MetaCore software, the variation in the expression of multiple pathways and networks in 3 h, 12 h, and 24 h after cerebral ischemia-reperfusion injury had been analyzed. The conserved TNFR1-signaling pathway, among the top 10 pathways, was consistently enriched in 3 h, 12 h, and 24 h groups. Three overlapping pathways were found between 3 h and 12 h groups; 2 between 12 h and 24 h groups; and 1 between 3 h and 24 h groups. Five, 4, and 6 non-overlapping pathways were observed in 3 h, 12 h, and 24 h groups, respectively. Apart from pathways reported by earlier studies, we identified a novel pathway related to the time-dependent development of cerebral ischemia pathogenesis. The process of apoptosis stimulation by external signals, among the top 10 processes, was consistently enriched in 3 h, 12 h, and 24 h groups; 2, 1, and 2 processes overlapped between 3 h and 12 h groups, 12 h and 24 h groups, and 3 h and 24 h groups, respectively. Four, 5, and 5 non-overlapping processes were found in 3 h, 12 h and 24 h groups, respectively. The presence of apoptotic processes was observed in all the 3 groups; while anti-apoptotic processes only existed in 3 h and 12 h groups. Additionally, according to node degree, network comparison identified 1, 8, and 5 important genes or proteins (e.g. Pyk2, PKC, E2F1, and VEGF-A) in 3 h, 12 h, and 24 h groups, respectively. The Jaccard similarity index revealed a higher level of similarity between 12 h and 24 h groups than that between 3 h and 12 h groups. Conclusion: Time-dependent treatment can be utilized to reduce apoptosis, which may activate anti-apoptotic pathways within 12 h after cerebral ischemia-reperfusion injury. Pathway and network analyses may help identify novel pathways and genes implicated in disease pathogenesis. [ABSTRACT FROM AUTHOR]
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- 2015
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13. The Comparative RNA Web (CRW) Site: an online database of comparative sequence and structure information for ribosomal, intron, and other RNAs.
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Cannone, Jamie J., Subramanian, Sankar, Schnare, Murray N., Collett, James R., D'Souza, Lisa M., Yushi Du, Feng, Brian, Nan Lin, Madabusi, Lakshmi V., Müller, Kirsten M., Pande, Nupur, Zhidi Shang, Nan Yu, and Gutell, Robin R.
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WEBSITES ,RNA ,COMPUTER network resources - Abstract
Background: Comparative analysis of RNA sequences is the basis for the detailed and accurate predictions of RNA structure and the determination of phylogenetic relationships for organisms that span the entire phylogenetic tree. Underlying these accomplishments are very large, well-organized, and processed collections of RNA sequences. This data, starting with the sequences organized into a database management system and aligned to reveal their higher-order structure, and patterns of conservation and variation for organisms that span the phylogenetic tree, has been collected and analyzed. This type of information can be fundamental for and have an influence on the study of phylogenetic relationships, RNA structure, and the melding of these two fields. Results: We have prepared a large web site that disseminates our comparative sequence and structure models and data. The four major types of comparative information and systems available for the three ribosomal RNAs (5S, 16S, and 23S rRNA), transfer RNA (tRNA), and two of the catalytic intron RNAs (group I and group II) are: (1) Current Comparative Structure Models; (2) Nucleotide Frequency and Conservation Information; (3) Sequence and Structure Data; and (4) Data Access Systems. Conclusions: This online RNA sequence and structure information, the result of extensive analysis, interpretation, data collection, and computer program and web development, is accessible at our Comparative RNA Web (CRW) Site [http://www.rna.icmb.utexas.edu] . In the future, more data and information will be added to these existing categories, new categories will be developed, and additional RNAs will be studied and presented at the CRW Site. [ABSTRACT FROM AUTHOR]
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- 2002
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14. HIV-1 uncoating in human CD4+ T cells: kinetic and functional analyses.
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Nan-Yu Chen, Lihong Zhou, Gane, Paul G., Price, Amanda, Zufferey, Madeleine, Luban, Jeremy, James, Leo, Selwood, David, and Fassati, Ariberto
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HIV , *CD4 antigen , *T cells - Abstract
An abstract of the article "HIV-1 uncoating in human CD4+ T cells: kinetic and functional analyses," by Nan Yu Chen and colleagues is presented.
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- 2013
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15. Clinical characteristics of acute hepatitis A outbreak in Taiwan, 2015-2016: observations from a tertiary medical center.
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Chen NY, Liu ZH, Shie SS, Chen TH, and Wu TS
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- AIDS-Related Opportunistic Infections epidemiology, AIDS-Related Opportunistic Infections virology, Adolescent, Adult, Aged, Aged, 80 and over, Child, Child, Preschool, Coinfection epidemiology, Comorbidity, Disease Outbreaks, Female, HIV Infections epidemiology, Hepatitis A etiology, Humans, Male, Middle Aged, Retrospective Studies, Risk Factors, Sexually Transmitted Diseases epidemiology, Syphilis epidemiology, Taiwan epidemiology, Young Adult, Hepatitis A epidemiology, Homosexuality, Male
- Abstract
Background: Acute hepatitis A is a fecal-oral transmitted disease related to inadequate sanitary conditions. In addition to its traditional classification, several outbreaks in the men who have sex with men (MSM) population have resulted in acute hepatitis A being recognized as a sexually transmitted disease. However, few studies have clarified the clinical manifestations in these outbreaks involving the MSM population., Methods: Beginning in June 2015, there was an outbreak of acute hepatitis A involving the MSM population in Northern Taiwan. We conducted a 15-year retrospective study by recruiting 207 patients with the diagnosis of acute hepatitis A that included the pre-outbreak (January 2001 to May 2015) and outbreak (June 2015 to August 2016) periods in a tertiary medical center in Northern Taiwan. Using risk factors, comorbidities, presenting symptoms, laboratory test results and imaging data, we aimed to evaluate the clinical significance of acute hepatitis A in the MSM population, where human immunodeficiency virus (HIV) coinfection is common., Results: There was a higher prevalence of reported MSM (p < 0.001), HIV (p < 0.001) and recent syphilis (p < 0.05) coinfection with acute hepatitis A during the outbreak period. The outbreak population had more prominent systemic symptoms, was more icteric with a higher total bilirubin level (p < 0.05) and had a 7-times higher tendency (p < 0.05) to have a hepatitis A relapse., Conclusions: The clinical course of acute hepatitis A during an outbreak involving the MSM and HIV-positive population is more symptomatic and protracted than in the general population.
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- 2017
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16. HIV-1 capsid is involved in post-nuclear entry steps.
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Chen NY, Zhou L, Gane PJ, Opp S, Ball NJ, Nicastro G, Zufferey M, Buffone C, Luban J, Selwood D, Diaz-Griffero F, Taylor I, and Fassati A
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- Aminocoumarins metabolism, Antiviral Agents metabolism, Cell Line, HIV-1 drug effects, Humans, HIV Core Protein p24 metabolism, HIV-1 physiology, Virus Internalization
- Abstract
Background: HIV-1 capsid influences viral uncoating and nuclear import. Some capsid is detected in the nucleus but it is unclear if it has any function. We reported that the antibiotic Coumermycin-A1 (C-A1) inhibits HIV-1 integration and that a capsid mutation confers resistance to C-A1, suggesting that capsid might affect post-nuclear entry steps., Results: Here we report that C-A1 inhibits HIV-1 integration in a capsid-dependent way. Using molecular docking, we identify an extended binding pocket delimited by two adjacent capsid monomers where C-A1 is predicted to bind. Isothermal titration calorimetry confirmed that C-A1 binds to hexameric capsid. Cyclosporine washout assays in Jurkat CD4+ T cells expressing engineered human TRIMCyp showed that C-A1 causes faster and greater escape from TRIMCyp restriction. Sub-cellular fractionation showed that small amounts of capsid accumulated in the nuclei of infected cells and C-A1 reduced the nuclear capsid. A105S and N74D capsid mutant viruses did not accumulate capsid in the nucleus, irrespective of C-A1 treatment. Depletion of Nup153, a nucleoporin located at the nuclear side of the nuclear pore that binds to HIV-1 capsid, made the virus less susceptible to TRIMCyp restriction, suggesting that Nup153 may help maintain some integrity of the viral core in the nucleus. Furthermore C-A1 increased binding of CPSF6, a nuclear protein, to capsid., Conclusions: Our results indicate that capsid is involved in post-nuclear entry steps preceding integration.
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- 2016
- Full Text
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