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Your search keyword '"Muhammad Farrukh"' showing total 11 results
Start Over Author "Muhammad Farrukh" Publisher biomed central
11 results on '"Muhammad Farrukh"'

5. Does the implementation of revised American College of Cardiology and American Heart Association (ACC/AHA) guidelines improve the identification of stillbirths and preterm births in hypertensive pregnancies: a population-based cohort study from South Asia and sub-Saharan Africa

Author

Nisar, Muhammad Imran, Kabole, Ibrahim, Khanam, Rasheda, Shahid, Shahira, Bakari, Bihila Abdalla, Chowdhury, Nabidul Haque, Qazi, Muhammad Farrukh, Dutta, Arup, Rahman, Sayedur, Khalid, Javairia, Dhingra, Usha, Hasan, Tarik, Ansari, Nadia, Deb, Saikat, Mitra, Dipak K., Mehmood, Usma, Aftab, Fahad, Ahmed, Salahuddin, Khan, Shahiryar, Ali, Said Mohammad, Ahmed, Saifuddin, Manu, Alexander, Yoshida, Sachiyo, Bahl, Rajiv, Baqui, Abdullah H., Sazawal, Sunil, and Jehan, Fyezah

Published
2024
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7. Early to mid-pregnancy HbA1c levels and its association with adverse pregnancy outcomes in three low middle-income countries in Asia and Sub-Saharan Africa

Author

Nisar, Muhammad Imran, das, Sayan, Khanam, Rasheda, Khalid, Javairia, Chetia, Swagata, Hasan, Tarik, Shahid, Shahira, Marijani, Msafiri Ladislaus, Ahmed, Salahuddin, Khalid, Farah, Ali, Said Mohammed, Chowdhury, Nabidul Haque, Mehmood, Usma, Dutta, Arup, Rahman, Sayedur, Qazi, Muhammad Farrukh, Deb, Saikat, Mitra, Dipak Kumar, Usmani, Asra Abeer, Dhingra, Usha, Raqib, Rubhana, Manu, Alexander, Yoshida, Sachiyo, Minckas, Nicole, Bahl, Rajiv, Baqui, Abdullah H., Sazawal, Sunil, and Jehan, Fyezah

Published
2024
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10. Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation

Author

Ping Ji, Julia Li Zhong, Chunxiang Bian, Shida Chen, Chang Ming Li, Lixia Gao, Yan Wu, Muhammad Farrukh Nisar, Ling Yu, and Enyi Huang

Subjects
0301 basic medicine, Environmental Engineering, Biomedical Engineering, Electrochemistry, UVR, 03 medical and health sciences, chemistry.chemical_compound, O2•−/ROS, Electrochemical sensing, medicine, Tocopherol, Irradiation, Molecular Biology, lcsh:QH301-705.5, Skin, chemistry.chemical_classification, Reactive oxygen species, Superoxide, Melanoma, Methodology, Biological activity, Cell Biology, medicine.disease, Electrochemical gas sensor, Anti-oxidant screening, 030104 developmental biology, chemistry, lcsh:Biology (General), O2 •−/ROS, Biophysics
Abstract

Background Generation of reactive oxygen species (ROS), triggered by ultraviolet radiation (UVR), is associated with carcinogenesis of the skin. UV irradiation induced superoxide anion (O2•−) is the key ROS involved in the cellular damage. The cytoprotective efficacy of an unknown anti-oxidant compound can be evaluated by analyzing the production of O2•− from treated cells. Methods In this study, a glass carbon electrode functionalized with nanotube@DNA-Mn3(PO4)2 composite was applied to quantitative determination of generation of highly unstable O2•− from the melanoma A375 cell line following UVR(UV, UVA and UVB). In addition, the cytoprotective efficacy of anti-oxidant α-tocopherol was evaluated by quantifying the production of O2•−. Results The results showed that, UVR triggers generation of O2•− in melanoma A375 cells, and α-tocopherol is effective in diminishing the production of O2•− following UV irradiation. By comparing the conventional cell-survival assays results, we found that our simple and quick electrochemical sensing method can quantify O2•− generation through the biological activity of an anti-oxidant compound (α-tocopherol). Conclusion Our label-free electrochemical quantification method for ROS (O2•− major) in cells facing UVR stress demonstrates its potential application for high-throughput screening of anti-oxidation compounds.

Published
2018

11. Heme oxygenase 1 facilitates cell proliferation via the B-Raf-ERK signaling pathway in melanoma.

Author

Liu, Liu, Wu, Yan, Bian, Chunxiang, Nisar, Muhammad Farrukh, Wang, Mei, Hu, Xiangyu, Diao, Qingchun, Nian, Weiqi, Wang, Enwen, Xu, Wei, and Zhong, Julia Li

Subjects
HEME oxygenase, CELL proliferation, MELANOMA, IMMUNOPRECIPITATION, IMMUNOHISTOCHEMISTRY, WESTERN immunoblotting
Abstract

Background: Despite therapeutic advancements (e.g. B-RAF inhibitors) targeting cutaneous melanoma, many cellular processes, including inducible heme oxygenase 1 (HO-1), counteract treatments for malignancies. So there is an urgent need to find biological treatment targets, develop new therapeutic approaches and achieve longer responses. This study aimed to explore the relationship of HO-1 and B-Raf via mediating ERK1/2 signaling on cell cycle in melanoma. Methods: Immunohistochemistry was applied to evaluate the levels of HO-1 and B-Raf expression in melanoma tissues and adjacent healthy tissues. Co-immunoprecipitation (Co-IP) assessed the interaction of HO-1 with B-Raf. Further study overexpression and knock-down of HO-1 in A375 cell lines, especially knockout HO-1 using CRISPR-Cas9, verified HO-1 regulate cell proliferation in vivo and in vitro. Finally, Western blot analysis and qRT-PCR were performed to investigate the mechanisms by which HO-1 mediates cell cycle by B-RAF-ERK1/2 signaling. Results: First, histology and Co-IP show that HO-1 interacts with B-Raf directly in melanoma tissue. Further study illustrated that HO-1 overexpression promotes melanoma cell proliferation while HO-1 reduction represses melanoma cell proliferation because of HO-1 affects cell cycle. Mechanistic studies revealed that HO-1 was associated with a marked activation of B-RAF-ERK1/2 signaling and led to CDK2/cyclin E activation, thereby promoting melanoma proliferation. Conclusions: Our result reveals a previously unknown mechanism that the HO-1-B-RAF-ERK axis plays an important role in melanoma cell proliferation. Therapeutic target on HO-1 could be a novel method for treating melanoma. [ABSTRACT FROM AUTHOR]

Published
2019
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