26 results on '"Moreno, Marta"'
Search Results
2. Maternal and obstetric outcomes after Ex-Utero Intrapartum Treatment (EXIT): a single center experience
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Domínguez-Moreno, Marta, Chimenea, Ángel, García-Díaz, Lutgardo, and Antiñolo, Guillermo
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- 2023
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3. Correction to: Advanced immunotherapies for glioblastoma: tumor neoantigen vaccines in combination with immunomodulators
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Segura-Collar, Berta, Hiller-Vallina, Sara, de Dios, Olaya, Caamaño-Moreno, Marta, Mondejar-Ruescas, Lucia, Sepulveda-Sanchez, Juan M., and Gargini, Ricardo
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- 2023
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4. Advanced immunotherapies for glioblastoma: tumor neoantigen vaccines in combination with immunomodulators
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Segura-Collar, Berta, Hiller-Vallina, Sara, de Dios, Olaya, Caamaño-Moreno, Marta, Mondejar-Ruescas, Lucia, Sepulveda-Sanchez, Juan M., and Gargini, Ricardo
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- 2023
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5. Nyssorhynchus darlingi genome-wide studies related to microgeographic dispersion and blood-seeking behavior
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Alvarez, Marcus Vinicius Niz, Alonso, Diego Peres, Kadri, Samir Moura, Rufalco-Moutinho, Paulo, Bernardes, Isabella Ariadne Ferrari, de Mello, Ana Carolina Florindo, Souto, Ana Carolina, Carrasco-Escobar, Gabriel, Moreno, Marta, Gamboa, Dionicia, Vinetz, Joseph M., Conn, Jan E., and Ribolla, Paulo E. M.
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- 2022
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6. Deletion patterns, genetic variability and protein structure of pfhrp2 and pfhrp3: implications for malaria rapid diagnostic test in Amhara region, Ethiopia
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Molina - de la Fuente, Irene, Yimar, Mulat, García, Luz, González, Vicenta, Amor, Arancha, Anegagrie, Melaku, Benito, Agustín, Martínez, Javier, Moreno, Marta, and Berzosa, Pedro
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- 2022
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7. Scalable transcriptomics analysis with Dask: applications in data science and machine learning
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Moreno, Marta, Vilaça, Ricardo, and Ferreira, Pedro G.
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- 2022
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8. The use of drones for mosquito surveillance and control
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Carrasco-Escobar, Gabriel, Moreno, Marta, Fornace, Kimberly, Herrera-Varela, Manuela, Manrique, Edgar, and Conn, Jan E.
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- 2022
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9. Maintaining Plasmodium falciparum gametocyte infectivity during blood collection and transport for mosquito feeding assays in the field
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Soumare, Harouna M., Guelbeogo, Wamdaogo Moussa, van de Vegte-Bolmer, Marga, van Gemert, Geert-Jan, Soumanaba, Zongo, Ouedraogo, Alphonse, Ouattara, Maurice S., Abdullahi, Ahmad, Jadama, Lamin, Camara, Muhammed M., Gaye, Pa Modou, Mendy, Michael, Davis, Nwakanma, Tiono, Alfred B., D’Alessandro, Umberto, Drakeley, Chris, Bousema, Teun, Moreno, Marta, and Collins, Katharine A.
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- 2021
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10. Higher risk of malaria transmission outdoors than indoors by Nyssorhynchus darlingi in riverine communities in the Peruvian Amazon
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Saavedra, Marlon P., Conn, Jan E., Alava, Freddy, Carrasco-Escobar, Gabriel, Prussing, Catharine, Bickersmith, Sara A., Sangama, Jorge L., Fernandez-Miñope, Carlos, Guzman, Mitchel, Tong, Carlos, Valderrama, Carlos, Vinetz, Joseph M., Gamboa, Dionicia, and Moreno, Marta
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- 2019
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11. Crosstalk between chromatin structure, cohesin activity and transcription
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Maya-Miles, Douglas, Andújar, Eloísa, Pérez-Alegre, Mónica, Murillo-Pineda, Marina, Barrientos-Moreno, Marta, Cabello-Lobato, María J., Gómez-Marín, Elena, Morillo-Huesca, Macarena, and Prado, Félix
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- 2019
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12. Decreasing proportion of Anopheles darlingi biting outdoors between long-lasting insecticidal net distributions in peri-Iquitos, Amazonian Peru
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Prussing, Catharine, Moreno, Marta, Saavedra, Marlon P., Bickersmith, Sara A., Gamboa, Dionicia, Alava, Freddy, Schlichting, Carl D., Emerson, Kevin J., Vinetz, Joseph M., and Conn, Jan E.
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- 2018
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13. The use of drones for mosquito surveillance and control
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Carrasco Escobar, Gabriel, Moreno, Marta, Fornace, Kimberly, Herrera Varela, Manuela, Manrique, Edgar, and Conn, Jan E.
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Uncrewed aerial vehicle ,Dengue ,Control ,Infectious diseases ,Drones ,Malaria - Abstract
In recent years, global health security has been threatened by the geographical expansion of vector-borne infectious diseases such as malaria, dengue, yellow fever, Zika and chikungunya. For a range of these vector-borne diseases, an increase in residual (exophagic) transmission together with ecological heterogeneity in everything from weather to local human migration and housing to mosquito species’ behaviours presents many challenges to effective mosquito control. The novel use of drones (or uncrewed aerial vehicles) may play a major role in the success of mosquito surveillance and control programmes in the coming decades since the global landscape of mosquito-borne diseases and disease dynamics fluctuates frequently and there could be serious public health consequences if the issues of insecticide resistance and outdoor transmission are not adequately addressed. For controlling both aquatic and adult stages, for several years now remote sensing data have been used together with predictive modelling for risk, incidence and detection of transmission hot spots and landscape profiles in relation to mosquito-borne pathogens. The field of drone-based remote sensing is under continuous change due to new technology development, operation regulations and innovative applications. In this review we outline the opportunities and challenges for integrating drones into vector surveillance (i.e. identification of breeding sites or mapping micro-environmental composition) and control strategies (i.e. applying larval source management activities or deploying genetically modified agents) across the mosquito life-cycle. We present a five-step systematic environmental mapping strategy that we recommend be undertaken in locations where a drone is expected to be used, outline the key considerations for incorporating drone or other Earth Observation data into vector surveillance and provide two case studies of the advantages of using drones equipped with multispectral cameras. In conclusion, recent developments mean that drones can be effective for accurately conducting surveillance, assessing habitat suitability for larval and/or adult mosquitoes and implementing interventions. In addition, we briefly discuss the need to consider permissions, costs, safety/privacy perceptions and community acceptance for deploying drone activities.
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- 2022
14. Crosstalk between chromatin structure, cohesin activity and transcription
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Ministerio de Economía y Competitividad (España), Junta de Andalucía, Maya-Miles, Douglas, Andújar, Eloísa, Pérez-Alegre, Mónica, Murillo-Pineda, Marina, Barrientos-Moreno, Marta, Cabello-Lobato, María J., Gómez-Marín, Elena, Morillo-Huesca, Macarena, Prado, Félix, Ministerio de Economía y Competitividad (España), Junta de Andalucía, Maya-Miles, Douglas, Andújar, Eloísa, Pérez-Alegre, Mónica, Murillo-Pineda, Marina, Barrientos-Moreno, Marta, Cabello-Lobato, María J., Gómez-Marín, Elena, Morillo-Huesca, Macarena, and Prado, Félix
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Background: A complex interplay between chromatin and topological machineries is critical for genome architecture and function. However, little is known about these reciprocal interactions, even for cohesin, despite its multiple roles in DNA metabolism. Results: We have used genome-wide analyses to address how cohesins and chromatin structure impact each other in yeast. Cohesin inactivation in scc1-73 mutants during the S and G2 phases causes specific changes in chromatin structure that preferentially take place at promoters; these changes include a significant increase in the occupancy of the - 1 and + 1 nucleosomes. In addition, cohesins play a major role in transcription regulation that is associated with specific promoter chromatin architecture. In scc1-73 cells, downregulated genes are enriched in promoters with short or no nucleosome-free region (NFR) and a fragile >nucleosome - 1/RSC complex> particle. These results, together with a preferential increase in the occupancy of nucleosome - 1 of these genes, suggest that cohesins promote transcription activation by helping RSC to form the NFR. In sharp contrast, the scc1-73 upregulated genes are enriched in promoters with an >open> chromatin structure and are mostly at cohesin-enriched regions, suggesting that a local accumulation of cohesins might help to inhibit transcription. On the other hand, a dramatic loss of chromatin integrity by histone depletion during DNA replication has a moderate effect on the accumulation and distribution of cohesin peaks along the genome. Conclusions: Our analyses of the interplay between chromatin integrity and cohesin activity suggest that cohesins play a major role in transcription regulation, which is associated with specific chromatin architecture and cohesin-mediated nucleosome alterations of the regulated promoters. In contrast, chromatin integrity plays only a minor role in the binding and distribution of cohesins.
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- 2019
15. Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns
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Moreno, Marta, Bickersmith, Sara, Harlow, Wesley, Hildebrandt, Jessica, Mckeon, Sascha N., Silva Do Nascimento, Teresa Fernandes, Loaiza, Jose R., Ruiz, Freddy, Lourenço de Oliveira, Ricardo, Sallum, María A. M., Bergo, Eduardo S., Fritz, Gary N., Wilkerson, Richard C., Dantur Juri, Maria Julia, Rangel, Yadira, Póvoa, Marinete M., Gutiérrez Builes, Lina A., Correa, Margarita M., and Conn, Jan E.
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WHITE GENE ,CIENCIAS MÉDICAS Y DE LA SALUD ,ANOPHELES TRIANNULATUS S S ,COI GENE ,Ciencias de la Salud ,Bioquímica y Biología Molecular ,PHYLOGEOGRAPHY ,Ciencias Biológicas ,purl.org/becyt/ford/1 [https] ,purl.org/becyt/ford/3.3 [https] ,ANOPHELES HALOPHYLUS ,Epidemiología ,Parasitología ,purl.org/becyt/ford/3 [https] ,purl.org/becyt/ford/1.6 [https] ,CIENCIAS NATURALES Y EXACTAS - Abstract
The molecular phylogenetic relationships and population structure of the species of the Anopheles triannulatus complex: Anopheles triannulatuss.s.,Anopheles halophylusand the putative species Anopheles triannulatus C were investigated. Methods:The mitochondrialCOIgene, the nuclearwhitegene and rDNA ITS2 of samples that include the known geographic distribution of these taxa were analyzed. Phylogenetic analyses were performed using Bayesian inference, Maximum parsimony and Maximum likelihood approaches. Results: Each data set analyzed separately yielded a different topology but none provided evidence for the separation ofAn. halophylusandAn. triannulatusC, consistent with the hypothesis that the two are undergoing incipient speciation. The phylogenetic analyses of the whitegene found three main clades, whereas the statistical parsimony network detected only a single metapopulation of Anopheles triannulatus s.l. Seven COI lineages were detected by phylogenetic and network analysis. In contrast, the network, but not the phylogenetic analyses,strongly supported three ITS2 groups. Combined data analyses provided the best resolution of the trees, with two major clades, Amazonian (clade I) and trans-Andean Amazon Delta(clade II). Clade I consists of multiple subclades: An. halophylus + An. triannulatus C; trans-Andean Venezuela; central Amazonia + central Bolivia; Atlantic coastal lowland; and Amazon delta. Clade II includes three subclades: Panama; cis-Andean Colombia; and cis-Venezuela. The Amazon delta specimens are in both clades, likely indicating local sympatry. Spatial and molecular variance analyses detected nine groups, corroborating some of subclades obtained in the combined data analysis. Conclusion:Combination of the three molecular markers provided the best resolution for differentiation within An.triannulatus s.s. and An. halophylusand C. The latest two species seem to be very closely related and the analyses performed were not conclusive regarding species differentiation. Further studies including new molecular markers would be desirable to solve this species status question. Besides, results of the study indicate a trans-Andean origin for An. triannulatus s.l. The potential implications for malaria epidemiology remain to be investigated. Fil: Moreno, Marta. New York State Department of Health. Wadsworth Center; Estados Unidos; Fil: Bickersmith, Sara. New York State Department of Health. Wadsworth Center; Estados Unidos; Fil: Harlow, Wesley. New York State Department of Health. Wadsworth Center; Estados Unidos; Fil: Hildebrandt, Jessica. New York State Department of Health. Wadsworth Center; Estados Unidos; Fil: Mckeon, Sascha N.. New York State Department of Health. Wadsworth Center; Estados Unidos; State University of New York. School of Public Health. Department of Biomedical Sciences; Estados Unidos; Fil: Silva Do Nascimento, Teresa Fernandes. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil; Fil: Loaiza, Jose R.. Instituto de Investigaciones Científicas y Servicios de Alta Tecnología; Panamá; Fil: Ruiz, Freddy. Walter Reed Army Institute of Research. Division of Entomology; Estados Unidos; Fil: Lourenço de Oliveira, Ricardo. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil; Fil: Sallum, María A. M.. Ministerio de Salud de Brasil. Fundacion Oswaldo Cruz. Departamento de Entomología; Brasil; Fil: Bergo, Eduardo S.. Superintendência de Controle de Endemias; Brazil; Fil: Fritz, Gary N.. Eastern Illinois University. Department of Biological Sciences; Estados Unidos; Fil: Wilkerson, Richard C.. Walter Reed Army Institute of Research. Division of Entomology; Estados Unidos; Fil: Dantur Juri, Maria Julia. Fundación Miguel Lillo. Dirección de Zoología; Argentina; Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Superior de Entomología; Argentina; Fil: Rangel, Yadira. Universidad Central de Venezuela. Instituto de Zoología y Ecología Tropical. Laboratorio de Biología de Vectores; Venezuela; Fil: Póvoa, Marinete M.. Instituto Evandro Chagas. Secção de Parasitologia; Brasil; Fil: Gutiérrez Builes, Lina A.. Universidad de Antioquia. Escuela de Microbiología. Grupo de Microbiología Molecular; Colombia; Fil: Correa, Margarita M.. Universidad de Antioquia. Escuela de Microbiología. Grupo de Microbiología Molecular; Colombia; Fil: Conn, Jan E.. New York State Department of Health. Wadsworth Center; Estados Unidos
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- 2013
16. Evidence for temporal population replacement and the signature of ecological adaptation in a major Neotropical malaria vector in Amazonian Peru.
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Lainhart, William, Bickersmith, Sara A., Nadler, Kyle J., Moreno, Marta, Saavedra, Marlon P., Chu, Virginia M., Ribolla, Paulo E., Vinetz, Joseph M., and Conn, Jan E.
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MALARIA treatment ,ANOPHELES ,MICROSATELLITE repeats ,PLASMODIUM genetics ,BIOMASS - Abstract
Background: The major Neotropical malaria vector, Anopheles darlingi, was reintroduced into the Iquitos, Loreto, Peru area during the early 1990s, where it displaced other anophelines and caused a major malaria epidemic. Since then, case numbers in Loreto have fluctuated, but annual increases have been reported since 2012. Methods: The population genetic structure of An. darlingi sampled before and after the introduction of long-lasting insecticidal nets (LLINs) was investigated to test the hypothesis of temporal population change (2006 vs. 2012). Current samples of An. darlingi were used to test the hypothesis of ecological adaptation to human modified (highway) compared with wild (riverine) habitat, linked to forest cover. In total, 693 An. darlingi from nine localities in Loreto, Peru area were genotyped using 13 microsatellite loci. To test the hypothesis of habitat differentiation in An. darlingi biting time patterns, HBR and EIR, four collections of An. darlingi from five localities (two riverine and three highway) were analysed. Results: Analyses of microsatellite loci from seven (2006) and nine settlements (2012-2014) in the Iquitos area detected two distinctive populations with little overlap, although it is unclear whether this population replacement event is associated with LLIN distribution or climate. Within the 2012-2014 population two admixed subpopulations, A and B, were differentiated by habitat, with B significantly overrepresented in highway, and both in near-equal proportions in riverine. Both subpopulations had a signature of expansion and there was moderate genetic differentiation between them. Habitat and forest cover level had significant effects on HBR, such that Plasmodium transmission risk, as measured by EIR, in peridomestic riverine settlements was threefold higher than in peridomestic highway settlements. HBR was directly associated with available host biomass rather than forest cover. Conclusions: A population replacement event occurred between 2006 and 2012-2014, concurrently with LLIN distribution and a moderate El Niño event, and prior to an increase in malaria incidence. The likely drivers of this replacement cannot be determined with current data. The present-day An. darlingi population is composed of two highly admixed subpopulations, which appear to be in an early stage of differentiation, triggered by anthropogenic alterations to local habitat. [ABSTRACT FROM AUTHOR]
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- 2015
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17. Implications for changes in Anopheles darlingi biting behaviour in three communities in the peri Iquitos region of Amazonian Peru.
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Moreno, Marta, Saavedra, Marlon P., Bickersmith, Sara A., Lainhart, William, Tong, Carlos, Alava, Freddy, Vinetz, Joseph M., and Conn, Jan E.
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ANOPHELES , *MOSQUITOES , *ENDEMIC diseases ,MALARIA transmission - Abstract
Background: Malaria transmission in the peri-Iquitos region of Amazonian Peru has been designated as seasonal and hypo-endemic with recently described hyper-endemic hotspots. Despite relatively recent distribution of long-lasting insecticidal bed nets (LLINs), malaria in Amazonian Peru persists and increased substantially in 2014 compared to previous years. Anopheles darlingi, identified as the main malaria vector, is known for its variable behaviour depending on locality and environment. Methods: To evaluate vector biology metrics in relation to seasonality and malaria transmission, mosquito collections were carried out in three localities in the peri-Iquitos region, Loreto, Peru in 2011-2012. Human landing catch (HLC) collection method, Shannon (SHA) and CDC trap types were compared for effectiveness in a neotropical setting. Abundance, human biting rate and entomological inoculation rate (EIR) were measured to provide an updated view of transmission patterns post-LLIN distribution. Results: HLC collected significantly more anopheline mosquitoes than SHA and CDC light traps. Anopheles darlingi was the most prevalent species in all three villages (84% overall). Biting patterns varied depending on trap type, season and village. EIR varied temporally (monthly) and spatially and the highest (2.52) occurred during the 2012 malaria outbreak in Cahuide. Unexpectedly there was a high infection rate (1.47 and 1.75) outside the normal malaria transmission season, coincident with a second local outbreak in Cahuide. The first identification of Anopheles dunhami and Anopheles oswaldoi C in Peru, using molecular markers, is also reported in this study. Conclusion: These data underscore the importance of HLC as the most meaningful collection method for measuring vector biology indices in this region. The highest monthly EIR provides additional evidence of seasonal transmission in riverine localities correlated with high river levels, and An. darlingi as the only contributor to transmission. The trend of an increase in outdoor-biting together with early-evening infected mosquitoes may undermine the effectiveness of LLINs as a primary malaria intervention. [ABSTRACT FROM AUTHOR]
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- 2015
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18. Demographic history and population structure of Anopheles pseudopunctipennis in Argentina based on the mitochondrial COI gene.
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Dantur Juri, María J., Moreno, Marta, Prado Izaguirre, Mónica J., Navarro, Juan C., Zaidenberg, Mario O., Almirón, Walter R., Claps, Guillermo L., and Conn, Jan E.
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Background: Anopheles pseudopunctipennis is an important malaria vector in the Neotropical region and the only species involved in Plasmodium transmission in the Andean foothills. Its wide geographical distribution in America, high preference for biting humans and capacity to rest inside dwellings after feeding, are attributes contributing to its vector status. Previous reports have tried to elucidate its taxonomic status, distinguishing populations from North, Central and South America. In the present study we used a mitochondrial marker to examine the demographic history of An. pseudopunctipennis in northwestern Argentina. Methods: Twelve localities were selected across 550 km of the distribution of this species in Argentina, including two near the Bolivian border and several in South Tucumán, for sampling. A fragment of the cytochrome oxidase I (COI) gene was sequenced and haplotype relationships were analyzed by a statistical parsimony network and a Neighbor-Joining (NJ) tree. Genetic differentiation was estimated with FST. Historical demographic processes were evaluated using diversity measures, neutrality tests and mismatch distribution. Results: Forty-one haplotypes were identified, of which haplotype A was the most common and widely distributed. Neither the network nor the NJ tree showed any geographic differentiation between northern and southern populations. Haplotype diversities, Tajima’s DT and Fu & Li’s F and D neutrality tests and mismatch distribution supported a scenario of Holocene demographic expansion. Conclusion: The demographic pattern suggests that An. pseudopunctipennis has undergone a single colonization process, and the ancestral haplotype is shared by specimens from all localities, indicating mitochondrial gene flow. Genetic differentiation was minimal, observed only between one northern and one southern locality. The estimated time of the population expansion of this species was during the Holocene. These data suggest that regional vector control measures would be equally effective in both northern and southern localities sampled, but also that insecticide resistant genes may spread rapidly within this region. [ABSTRACT FROM AUTHOR]
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- 2014
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19. Malaria Panel Assay versus PCR: detection of naturally infected Anopheles melas in a coastal village of Equatorial Guinea
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Moreno, Marta, Cano, Jorge, Nzambo, Sisinio, Bobuakasi, Leonardo, Buatiche, Jesús N, Ondo, Melchor, Micha, Francisco, Benito, Agustín, Agencia Española de Cooperación Internacional, Instituto de Salud Carlos III, Red de Investigación Cooperativa en Enfermedades Tropicales (España), and Agencia Española de Cooperación Internacional para el Desarrollo
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lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,Research ,Plasmodium falciparum ,Enzyme-Linked Immunosorbent Assay ,DNA, Protozoan ,Polymerase Chain Reaction ,Sensitivity and Specificity ,lcsh:Infectious and parasitic diseases ,Insect Vectors ,parasitic diseases ,Anopheles ,Equatorial Guinea ,Animals ,Humans ,lcsh:RC109-216 ,Female ,Malaria, Falciparum - Abstract
BACKGROUND: A study was carried out in a village of the mainland region of Equatorial Guinea in order to ascertain a) which members of Anopheles gambiae complex could be involved in malaria transmission and b) the rate of infectivity for Anopheles melas comparing two different methods, a PCR able to detect sporozoite-DNA and an immunochromatographic assay MPR (Malaria Rapid Dipstick Panel Assay). METHODS: Mosquitoes were sampled at night by indoor captures in two houses of a coastal village in Equatorial Guinea (Ayantang). Collected mosquitoes were identified as An. gambiae s.l. These were individually dried into silica-gel. The head-thorax of the An. gambiae s.l. mosquitoes were analysed by PCR to verify that the species was of the gambiae complex. Individual head-thorax and pools (5 pools) of homogenized mosquitoes employed in Malaria Rapid Panel assay (MRP assay) were lysed and DNA was extracted. PCR was designed from the 753 base pair insert of pBRKl-14 and DNA was amplified. The relationship between dipstick and PCR to detect Plasmodium falciparum sporozoites was measured in terms of sensitivity, specificity and test association (Cohen's kappa value). RESULTS: Two hundred and sixty-four An. gambiae s.l. females were studied (214 individually and five pools with 10 mosquitoes in each). PCR analysis showed that 207 mosquitoes were An. melas, 3 An. gambiae s.s. and 4 could not be identified. By using PCR as the gold standard method when dipstick assay was compared, matching results were obtained for 6 mosquitoes and, in one case MRP was positive while PCR was not reactive. MRP assay showed a low sensitivity (3.3%) when compared with falciparum-DNA detection (17,7% and 14,3%, series A and B respectively). Agreement between the two test formats was low (kappa = 0,224). CONCLUSION: It was determined that An. melas is the main anopheline vector involved in malaria transmission in Ayantang, a coastal village in mainland Equatorial Guinea. A comparison of PCR and Vec-Test Assay, concluded that the PCR method proved to be a more sensitive and useful tool than the dipstick assay to determine the malarial infection rate in mosquitoes in an area of stable and high malaria transmission like Equatorial Guinea. This work was supported by the Spanish Agency of International Cooperation (AECI), the Institute of Health Carlos III and by the Research Network of Centres for Tropical Medicine (RICET), supported by FIS. Nick Service made comments on the manuscript and helped with the language revision. Sí
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- 2004
20. Id1 and Id3 co-expression correlates with clinical outcome in stage III-N2 non-small cell lung cancer patients treated with definitive chemoradiotherapy.
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Castañon, Eduardo, Bosch-Barrera, Joaquim, López, Inés, Collado, Víctor, Moreno, Marta, López-Picazo, José María, Arbea, Leire, Lozano, María Dolores, Calvo, Alfonso, and Gil-Bazo, Ignacio
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LUNG cancer patients ,IMMUNOHISTOCHEMISTRY ,MONOCLONAL antibodies ,STATISTICAL correlation ,HEALTH outcome assessment ,RADIOTHERAPY - Abstract
Background: Inhibitor of DNA binding 1 (Id1) and 3 (Id3) genes have been related with the inhibition of cell differentiation, cell growth promotion and tumor metastasis. Recently, Id1 has been identified as an independent prognostic factor in patients with lung adenocarcinoma, regardless of the stage. Furthermore, Id1 may confer resistance to treatment (both, radiotherapy and chemotherapy). Methods: We have studied, using monoclonal antibodies for immunohistochemistry, the Id1 and Id3 tumor epithelial expression in 17 patients with stage III-N2 non-small cell lung cancer (NSCLC) treated with definitive chemoradiotherapy. Results: Id1 expression is observed in 82.4% of the tumors, whereas Id3 expression is present in 41.2% of the samples. Interestingly, Id1 and Id3 expression are mutually correlated (R = 0.579, p = 0.015). In a subgroup analysis of patients with the most locally advanced disease (T4N2 stage), co-expression of Id1 and Id3 showed to be related with a worse overall survival (45 vs 6 months, p = 0.002). A trend towards significance for a worse progression free survival (30 vs 1 months, p = 0.219) and a lower response rate to the treatment (RR = 50% vs 87.5%, p = 0.07) were also observed. Conclusions: A correlation between Id1 and Id3 protein expression is observed. Id1 and Id3 co-expression seems associated with a poor clinical outcome in patients with locally advanced NSCLC treated with definitive chemoradiotherapy. [ABSTRACT FROM AUTHOR]
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- 2013
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21. Complete mtDNA genomes of Anopheles darlingi and an approach to anopheline divergence time.
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Moreno, Marta, Marinotti, Osvaldo, Krzywinski, Jaroslaw, Tadei, Wanderli P., James, Anthony A., Achee, Nicole L., and Conn, Jan E.
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ANOPHELES , *MALARIA , *PUBLIC health , *DNA - Abstract
Background: The complete sequences of the mitochondrial genomes (mtDNA) of members of the northern and southern genotypes of Anopheles (Nyssorhynchus) darlingi were used for comparative studies to estimate the time to the most recent common ancestor for modern anophelines, to evaluate differentiation within this taxon, and to seek evidence of incipient speciation. Methods: The mtDNAs were sequenced from mosquitoes from Belize and Brazil and comparative analyses of structure and base composition, among others, were performed. A maximum likelihood approach linked with phylogenetic information was employed to detect evidence of selection and a Bayesian approach was used to date the split between the subgenus Nyssorhynchus and other Anopheles subgenera. Results: The comparison of mtDNA sequences within the Anopheles darlingi taxon does not provide sufficient resolution to establish different units of speciation within the species. In addition, no evidence of positive selection in any protein-coding gene of the mtDNA was detected, and purifying selection likely is the basis for this lack of diversity. Bayesian analysis supports the conclusion that the most recent ancestor of Nyssorhynchus and Anopheles+Cellia was extant ∼94 million years ago. Conclusion: Analyses of mtDNA genomes of Anopheles darlingi do not provide support for speciation in the taxon. The dates estimated for divergence among the anopheline groups tested is in agreement with the geological split of western Gondwana (95 mya), and provides additional support for explaining the absence of Cellia in the New World, and Nyssorhynchus in the Afro-Eurasian continents. [ABSTRACT FROM AUTHOR]
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- 2010
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22. Intensity-modulated radiation therapy (IMRT) vs. 3D conformal radiotherapy (3DCRT) in locally advanced rectal cancer (LARC): dosimetric comparison and clinical implications.
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Arbea, Leire, Ramos, Luis Isaac, Martínez-Monge, Rafael, Moreno, Marta, and Aristu, Javier
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RECTAL cancer treatment ,CANCER radiotherapy ,MEDICAL dosimetry ,DRUG dosage ,DRUG prescribing - Abstract
Purpose: To compare target dose distribution, comformality, normal tissue avoidance, and irradiated body volume (IBV) in 3DCRT using classic anatomical landmarks (c3DCRT), 3DCRT fitting the PTV (f3DCRT), and intensitymodulated radiation therapy (IMRT) in patients with locally advanced rectal cancer (LARC). Materials and methods: Fifteen patients with LARC underwent c3DCRT, f3DCRT, and IMRT planning. Target definition followed the recommendations of the ICRU reports No. 50 and 62. OAR (SB and bladder) constraints were D5 ⩽ 50 Gy and Dmax < 55 Gy. PTV dose prescription was defined as PTV95 ⩾ 45 Gy and PTVmin ⩾ 35 Gy. Target coverage was evaluated with the D95, Dmin, and Dmax. Target dose distribution and comformality was evaluated with the homogeneity indices (HI) and Conformity Index (CI). Normal tissue avoidance of OAR was evaluated with the D5 and V40. IBV at 5 Gy (V5), 10 Gy (V10), and 20 Gy (V20) were calculated. Results: The mean GTV95, CTV95, and PTV95 doses were significantly lower for IMRT plans. Target dose distribution was more inhomogeneous after IMRT planning and 3DCRTplans had significantly lower CI. The V40 and D5 values for OAR were significantly reduced in the IMRT plans .V5 was greater for IMRT than for f3DCRT planning (p < 0.05) and V20 was smaller for IMRT plans(p < 0.05). Conclusions: IMRT planning improves target conformity and decreases irradiation of the OAR at the expense of increased target heterogeneity. IMRT planning increases the IBV at 5 Gy or less but decreases the IBV at 20 Gy or more. [ABSTRACT FROM AUTHOR]
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- 2010
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23. Genetic population structure of Anopheles gambiae in Equatorial Guinea.
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Moreno, Marta, Salgueiro, Patricia, Vicente, José Luis, Cano, Jorge, Berzosa, Pedro J., Lucio, Aida de, Simard, Frederic, Caccone, Adalgisa, Do Rosario, Virgilio E., Pinto, João, and Benito, Agustín
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POPULATION genetics , *ANOPHELES gambiae , *PREVENTION of communicable diseases , *CELL nuclei , *VECTOR control , *MOSQUITO vectors - Abstract
Background: Patterns of genetic structure among mosquito vector populations in islands have received particular attention as these are considered potentially suitable sites for experimental trials on transgenic-based malaria control strategies. In this study, levels of genetic differentiation have been estimated between populations of Anopheles gambiae s.s. from the islands of Bioko and Annobón, and from continental Equatorial Guinea (EG) and Gabon. Methods: Genotyping of 11 microsatellite loci located in chromosome 3 was performed in three island samples (two in Bioko and one in Annobón) and three mainland samples (two in EG and one in Gabon). Four samples belonged to the M molecular form and two to the S-form. Micro-satellite data was used to estimate genetic diversity parameters, perform demographic equilibrium tests and analyse population differentiation. Results: High levels of genetic differentiation were found between the more geographically remote island of Annobón and the continent, contrasting with the shallow differentiation between Bioko island, closest to mainland, and continental localities. In Bioko, differentiation between M and S forms was higher than that observed between island and mainland samples of the same molecular form. Conclusion: The observed patterns of population structure seem to be governed by the presence of both physical (the ocean) and biological (the M-S form discontinuity) barriers to gene flow. The significant degree of genetic isolation between M and S forms detected by microsatellite loci located outside the "genomic islands" of speciation identified in A. gambiae s.s. further supports the hypothesis of on-going incipient speciation within this species. The implications of these findings regarding vector control strategies are discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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24. Spatial variability in the density, distribution and vectorial capacity of anopheline species in a high transmission village (Equatorial Guinea).
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Cano, Jorge, Descalzo, Miguel Ángel, Moreno, Marta, Zhaoguo Chen, Nzambo, Sisinio, Bobuakasi, Leonardo, Buatiche, Jesús N., Ondo, Melchor, Micha, Francisco, and Benito, Agustín
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MALARIA transmission ,ANIMAL breeding ,ENTOMOLOGY ,PLASMODIUM falciparum - Abstract
Background: Malaria transmission varies from one country to another and there are also local differences in time and space. An important variable when explaining the variability in transmission is the breeding behaviour of the different vector species and the availability of breeding sites. The aim of this study was to determine the geographical variability of certain entomological parameters: human biting rate (HBR), sporozoitic index (SI) for Plasmodium falciparum and entomological inoculation rate (EIR). Methods: The study was carried out in a small village in the mainland region of Equatorial Guinea. Adult mosquitoes were collected by CDC light traps. Polymerase Chain Reaction was employed to identify the species within the Anopheles gambiae complex and to detect P. falciparum sporozoites. The geographical position of all the dwellings in the village were taken using a global positioning system receiver unit. Data relating to the dwelling, occupants, use of bednets and the mosquitoes collection data were used to generate a geographical information system (GIS). This GIS allowed the minimum distance of the dwellings to the closest water point (potential breeding sites) to be determined. Results: A total of 1,173 anophelines were caught: 279 A. gambiae s.l. (217 A. gambiae s.s. and ne Anopheles melas), 777 Anopheles moucheti and 117 Anopheles carnevalei. A. moucheti proved to be the main vector species and was responsible for 52.38 [95% IC: 33.7-71] night infective bites during this period. The highest SI was found in A. carnevalei (24%), even though the HBR was the lowest for this species. A significant association was found between the distance from the dwellings to the closest water point (River Ntem or secondary streams) and the total HBR. Conclusion: A clear association has been observed between the distance to potential breeding sites and the variability in the anopheline density, while the other parameters measured do not seem to condition this spatial variability. The application of GIS to the study of vector-transmitted diseases considerably improves the management of the information obtained from field surveys and facilitates the study of the distribution patterns of the vector species. [ABSTRACT FROM AUTHOR]
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- 2006
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25. Decreasing proportion of <italic>Anopheles darlingi</italic> biting outdoors between long-lasting insecticidal net distributions in peri-Iquitos, Amazonian Peru.
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Prussing, Catharine, Moreno, Marta, Saavedra, Marlon P., Bickersmith, Sara A., Gamboa, Dionicia, Alava, Freddy, Schlichting, Carl D., Emerson, Kevin J., Vinetz, Joseph M., and Conn, Jan E.
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MALARIA prevention ,ANOPHELES ,GENETICS ,RISK of malaria ,POPULATION genetics - Abstract
Background: In Loreto Department, Peru, a successful 2005–2010 malaria control programme (known as PAMAFRO) included massive distribution of long-lasting insecticidal nets (LLINs). Additional local distribution of LLINs occurred in individual villages, but not between 2012 and 2015. A 2011–2012 study of the primary regional malaria vector
Anopheles darlingi detected a trend of increased exophagy compared with pre-PAMAFRO behaviour. For the present study,An. darlingi were collected in three villages in Loreto in 2013–2015 to test two hypotheses: (1) that between LLIN distributions,An. darlingi reverted to pre-intervention biting behaviour; and, (2) that there are separate sub-populations ofAn. darlingi in Loreto with distinct biting behaviour. Results: In 2013–2015An. darlingi were collected by human landing catch during the rainy and dry seasons in the villages of Lupuna and Cahuide. The abundance ofAn. darlingi varied substantially across years, villages and time periods, and there was a twofold decrease in the ratio of exophagic:endophagicAn. darlingi over the study period. Unexpectedly, there was evidence of a rainy season population decline inAn. darlingi .Plasmodium -infectedAn. darlingi were detected indoors and outdoors throughout the night, and the monthlyAn. darlingi human biting rate was correlated with the number of malaria cases. Using nextRAD genotyping-by-sequencing, 162 exophagic and endophagicAn. darlingi collected at different times during the night were genotyped at 1021 loci. Based on model-based and non-model-based analyses, all genotypedAn. darlingi belonged to a homogeneous population, with no evidence for genetic differentiation by biting location or time. Conclusions: This study identified a decreasing proportion of exophagicAn. darlingi in two villages in the years between LLIN distributions. As there was no evidence for genetic differentiation between endophagic and exophagicAn. darlingi , this shift in biting behaviour may be the result of behavioural plasticity inAn. darlingi , which shifted towards increased exophagy due to repellence by insecticides used to impregnate LLINs and subsequently reverted to increased endophagy as the nets aged. This study highlights the need to target vector control interventions to the biting behaviour of local vectors, which, like malaria risk, shows high temporal and spatial heterogeneity. [ABSTRACT FROM AUTHOR]- Published
- 2018
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26. Phylogeography of the neotropical Anopheles triannulatus complex (Diptera: Culicidae) supports deep structure and complex patterns.
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Moreno M, Bickersmith S, Harlow W, Hildebrandt J, McKeon SN, Silva-do-Nascimento TF, Loaiza JR, Ruiz F, Lourenço-de-Oliveira R, Sallum MA, Bergo ES, Fritz GN, Wilkerson RC, Linton YM, Juri MJ, Rangel Y, Póvoa MM, Gutiérrez-Builes LA, Correa MM, and Conn JE
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- Animals, Anopheles classification, Base Sequence, Bayes Theorem, DNA, Mitochondrial chemistry, DNA, Mitochondrial genetics, DNA, Ribosomal Spacer chemistry, DNA, Ribosomal Spacer genetics, Electron Transport Complex IV genetics, Genetic Markers genetics, Haplotypes, Humans, Insect Vectors classification, Mitochondrial Proteins genetics, Molecular Sequence Data, Phylogeography, Sequence Analysis, DNA, South America, Anopheles genetics, Genetic Variation, Insect Vectors genetics, Malaria transmission
- Abstract
Background: The molecular phylogenetic relationships and population structure of the species of the Anopheles triannulatus complex: Anopheles triannulatus s.s., Anopheles halophylus and the putative species Anopheles triannulatus C were investigated., Methods: The mitochondrial COI gene, the nuclear white gene and rDNA ITS2 of samples that include the known geographic distribution of these taxa were analyzed. Phylogenetic analyses were performed using Bayesian inference, Maximum parsimony and Maximum likelihood approaches., Results: Each data set analyzed septely yielded a different topology but none provided evidence for the seption of An. halophylus and An. triannulatus C, consistent with the hypothesis that the two are undergoing incipient speciation. The phylogenetic analyses of the white gene found three main clades, whereas the statistical parsimony network detected only a single metapopulation of Anopheles triannulatus s.l. Seven COI lineages were detected by phylogenetic and network analysis. In contrast, the network, but not the phylogenetic analyses, strongly supported three ITS2 groups. Combined data analyses provided the best resolution of the trees, with two major clades, Amazonian (clade I) and trans-Andean + Amazon Delta (clade II). Clade I consists of multiple subclades: An. halophylus + An. triannulatus C; trans-Andean Venezuela; central Amazonia + central Bolivia; Atlantic coastal lowland; and Amazon delta. Clade II includes three subclades: Panama; cis-Andean Colombia; and cis-Venezuela. The Amazon delta specimens are in both clades, likely indicating local sympatry. Spatial and molecular variance analyses detected nine groups, corroborating some of subclades obtained in the combined data analysis., Conclusion: Combination of the three molecular markers provided the best resolution for differentiation within An. triannulatus s.s. and An. halophylus and C. The latest two species seem to be very closely related and the analyses performed were not conclusive regarding species differentiation. Further studies including new molecular markers would be desirable to solve this species status question. Besides, results of the study indicate a trans-Andean origin for An. triannulatus s.l. The potential implications for malaria epidemiology remain to be investigated.
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- 2013
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