7 results on '"Ma, Tengfei"'
Search Results
2. NLRP3 deficiency decreases alcohol intake controlling anxiety-like behavior via modification of glutamatergic transmission in corticostriatal circuits
- Author
-
Li, Ziyi, Vidjro, Olivia Ewi, Guo, Gengni, Du, Yanfeng, Zhou, Yao, Xie, Qian, Li, Jiaxin, Gao, Keqiang, Zhou, Li, and Ma, Tengfei
- Published
- 2022
- Full Text
- View/download PDF
3. Comparative transcriptomic analysis of the flower induction and development of the Lei bamboo (Phyllostachys violascens)
- Author
-
Jiao, Yulian, Hu, Qiutao, Zhu, Yan, Zhu, Longfei, Ma, Tengfei, Zeng, Haiyong, Zang, Qiaolu, Li, Xuan, and Lin, Xinchun
- Published
- 2019
- Full Text
- View/download PDF
4. Burkholderia pseudomallei-derived miR-3473 enhances NF-κB via targeting TRAF3 and is associated with different inflammatory responses compared to Burkholderia thailandensis in murine macrophages
- Author
-
Hu Yi, Ma Tengfei, Hai Chen, Yao Fang, Zhiqiang Hu, Xuhu Mao, and Qian Li
- Subjects
0301 basic medicine ,TRAF3 ,Microbiology (medical) ,Burkholderia pseudomallei (Bp) ,miR-3473 ,Burkholderia pseudomallei ,Burkholderia ,030106 microbiology ,Virulence ,Apoptosis ,Microbiology ,NF-κB ,Cell Line ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Burkholderia thailandensis (Bt) ,Animals ,Mice, Inbred BALB C ,Burkholderia thailandensis ,biology ,TNF Receptor-Associated Factor 3 ,Tumor Necrosis Factor-alpha ,Macrophages ,NF-kappa B ,Burkholderia Infections ,biology.organism_classification ,MicroRNAs ,030104 developmental biology ,RAW 264.7 Cells ,chemistry ,TNF-α ,Host-Pathogen Interactions ,Tumor necrosis factor alpha ,Intracellular ,Research Article - Abstract
Background Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a kind of tropical disease. Burkholderia thailandensis (Bt), with a high sequence similarity to Bp, is thought to be an avirulent organism. Since there are numerous similarities between Bp and Bt, their differences in pathogenesis of host response and related mechanism are still undermined. In recent years, microRNAs have been researched in many diseases, but seldom involved in bacterial infection, bacteria-host interaction or explaining the differences between virulent and avirulent species. Results We found that Bp and Bt had similar phenotypes in terms of intracellular replication, dissemination (reflected by multinucleated giant cell formation), TNF-α release and apoptosis in RAW264.7 macrophages or TC-1 pulmonary cell but in different level. Especially, at the late infection phases (after 12 h post infection), Bp showed faster intracellular growth, stronger cytotoxicity, and higher TNF-α release. After microRNA array analysis, we found some microRNAs were significantly expressed in macrophages treated by Bp. miR-3473 was one of them specifically induced, but not significantly changed in Bt-treated macrophages. In addition, TargetScan suggested that miR-3473 possibly target TRAF3 (TNF receptor-associated factor 3), a well-known negative regulator of the NF-κB pathway, which was probably involved in the TNF-α induction and apoptosis in cells with Bp infection. In vivo, it was found that miR-3473 expression of total lungs cells from Bp-treated was higher than that from Bt-treated mice. And miR-3473 inhibitor was able to decrease the TNF-α release of mice and prolong the survival of mice with Bp infection. Conclusion In sum, miR-3473 plays an important role in the differential pathogenicity of Bp and Bt via miR-3473-TRAF3-TNF-α network, and regulates TNF-α release, cell apoptosis and animal survival after Bp treatment. In this study, we have found a specific microRNA is related to bacterial virulence and provide insight into the mechanism for host-bacteria interaction, which suggests that potential oligonucleotides should be applied against bacterial infection.
- Published
- 2016
5. Identification and analysis of the stigma and embryo sac-preferential/specific genes in rice pistils.
- Author
-
Yu L, Ma T, Zhang Y, Hu Y, Yu K, Chen Y, Ma H, and Zhao J
- Subjects
- Arabidopsis genetics, Arabidopsis metabolism, Flowers metabolism, Gene Expression Profiling, Oryza metabolism, Pollen genetics, Pollen metabolism, Flowers genetics, Oryza genetics
- Abstract
Background: In rice, the pistil is the female reproductive organ, and it consists of two stigmas and an ovary. The stigma is capable of receiving pollen grains and guiding pollen tube growth. The ovary holds the embryo sac, which is fertilized with male gametes to produce seed. However, little is known about the gene function and regulatory networks during these processes in rice., Results: Here, using the RNA-Seq technique, we identified 3531 stigma-preferential genes and 703 stigma-specific genes within the rice pistils, and we verified 13 stigma-specific genes via qRT-PCR and in situ hybridization. The GO analysis showed that the transport-, localization-, membrane-, communication-, and pollination-related genes were significantly enriched in the stigma. Additionally, to identify the embryo sac-preferential/specific genes within the pistils, we compared a wild-type ovary with a mutant dst (defective stigma) ovary and found that 385 genes were down-regulated in dst. Among these genes, 122 exhibited an ovary-specific expression pattern and are thought to be embryo sac-preferential/specific genes within the pistils. Most of them were preferentially expressed, while 14 of them were specifically expressed in the pistil. Moreover, the rice homologs of some Arabidopsis embryo sac-specific genes, which played essential roles during sexual reproduction, were down-regulated in dst. Additionally, we identified 102 novel protein-coding genes, and 6 of them exhibited differences between the stigma and ovary in rice as determined using RT-PCR., Conclusions: According to these rice ovary comparisons, numerous genes were preferentially or specifically expressed in the stigma, suggesting that they were involved in stigma development or pollination. The GO analysis indicated that a dry rice stigma might primarily perform its function through the cell membrane, which was different from the wet stigma of other species. Moreover, many embryo sac-preferential/specific genes within the pistils were identified and may be expressed in female rice gametophytes, implying that these genes might participate in the process of female gametophyte specialization and fertilization. Therefore, we provide the gene information for investigating the gene function and regulatory networks during female gametophyte development and fertilization. In addition, these novel genes are valuable for the supplementation and perfection of the existing transcriptome in rice, which provides an effective method of detecting novel rice genes.
- Published
- 2017
- Full Text
- View/download PDF
6. Burkholderia pseudomallei-derived miR-3473 enhances NF-κB via targeting TRAF3 and is associated with different inflammatory responses compared to Burkholderia thailandensis in murine macrophages.
- Author
-
Fang Y, Chen H, Hu Y, Li Q, Hu Z, Ma T, and Mao X
- Subjects
- Animals, Apoptosis physiology, Burkholderia pathogenicity, Burkholderia pseudomallei genetics, Burkholderia pseudomallei metabolism, Burkholderia pseudomallei pathogenicity, Cell Line, Host-Pathogen Interactions, Macrophages immunology, Mice, Mice, Inbred BALB C, RAW 264.7 Cells, Tumor Necrosis Factor-alpha metabolism, Burkholderia metabolism, Burkholderia Infections microbiology, Burkholderia pseudomallei physiology, Macrophages microbiology, MicroRNAs biosynthesis, NF-kappa B metabolism, TNF Receptor-Associated Factor 3 metabolism
- Abstract
Background: Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a kind of tropical disease. Burkholderia thailandensis (Bt), with a high sequence similarity to Bp, is thought to be an avirulent organism. Since there are numerous similarities between Bp and Bt, their differences in pathogenesis of host response and related mechanism are still undermined. In recent years, microRNAs have been researched in many diseases, but seldom involved in bacterial infection, bacteria-host interaction or explaining the differences between virulent and avirulent species., Results: We found that Bp and Bt had similar phenotypes in terms of intracellular replication, dissemination (reflected by multinucleated giant cell formation), TNF-α release and apoptosis in RAW264.7 macrophages or TC-1 pulmonary cell but in different level. Especially, at the late infection phases (after 12 h post infection), Bp showed faster intracellular growth, stronger cytotoxicity, and higher TNF-α release. After microRNA array analysis, we found some microRNAs were significantly expressed in macrophages treated by Bp. miR-3473 was one of them specifically induced, but not significantly changed in Bt-treated macrophages. In addition, TargetScan suggested that miR-3473 possibly target TRAF3 (TNF receptor-associated factor 3), a well-known negative regulator of the NF-κB pathway, which was probably involved in the TNF-α induction and apoptosis in cells with Bp infection. In vivo, it was found that miR-3473 expression of total lungs cells from Bp-treated was higher than that from Bt-treated mice. And miR-3473 inhibitor was able to decrease the TNF-α release of mice and prolong the survival of mice with Bp infection., Conclusion: In sum, miR-3473 plays an important role in the differential pathogenicity of Bp and Bt via miR-3473-TRAF3-TNF-α network, and regulates TNF-α release, cell apoptosis and animal survival after Bp treatment. In this study, we have found a specific microRNA is related to bacterial virulence and provide insight into the mechanism for host-bacteria interaction, which suggests that potential oligonucleotides should be applied against bacterial infection.
- Published
- 2016
- Full Text
- View/download PDF
7. Identification, characterization, and transcription analysis of xylogen-like arabinogalactan proteins in rice (Oryza sativa L.).
- Author
-
Ma T, Ma H, Zhao H, Qi H, and Zhao J
- Subjects
- Gene Expression Regulation, Plant, Mucoproteins genetics, Oryza genetics, Plant Proteins genetics
- Abstract
Background: Xylogen, a chimeric arabinogalactan protein containing a non-specific lipid transfer protein domain, can promote xylem cell differentiation. No comprehensive study has been carried out on the XYLP gene family in rice. As a first step in research on this gene family and as a useful strategy in general, a genome-wide analysis of the OsXYLP gene family is thus needed., Results: In this study, we identified 21 XYLP genes from the rice genome and comprehensively analyzed their protein structures, phylogenetic relationships, chromosomal locations, and gene duplication status. Our results indicate that gene duplication has played major roles in the expansion of the OsXYLP gene family. We used expressed sequence tag, microarray, massively parallel signature sequencing, and quantitative real-time PCR data to analyze OsXYLP gene expression during various developmental stages and under abiotic stress conditions. We found that many OsXYLP genes are abundantly expressed in vascular tissues and seeds, with some genes regulated under hormonal or abiotic stresses. In addition, we identified knockout mutants of OsXYLP7 and OsXYLP16 and discovered that the mutant xylp7 has a defect in stem height., Conclusions: We analyzed expression profiles of 21 XYLP genes and characterized the structures and evolutionary relationships of their proteins. Our results demonstrate that the rice XYLP gene family may play roles in plant vascular system development and hormone signaling. Among the 21 detected OsXYLPs, 19 are newly identified genes encoding arabinogalactan proteins. Our results provide comprehensive insights that will assist future research on the biological functions of the rice XYLP gene family.
- Published
- 2014
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.