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Start Over Author "Ling, Ning" Publisher biomed central
4 results on '"Ling, Ning"'

4. MicroRNA-182 modulates chemosensitivity of human non-small cell lung cancer to cisplatin by targeting PDCD4

Author

Fang-ling Ning, Yan-zhang Hao, Feng Wang, Shao-shui Chen, Ze-shun Yu, and Mian-li Li

Subjects
Histology, Lung Neoplasms, Blotting, Western, Antineoplastic Agents, Biology, NSCLC, Real-Time Polymerase Chain Reaction, Pathology and Forensic Medicine, A549, Western blot, Carcinoma, Non-Small-Cell Lung, Cell Line, Tumor, medicine, Humans, MTT assay, Lung cancer, Chemosensitivity, miRNA, A549 cell, Cisplatin, medicine.diagnostic_test, PDCD4, Reverse Transcriptase Polymerase Chain Reaction, Research, RNA-Binding Proteins, General Medicine, Transfection, medicine.disease, Molecular biology, Blot, Gene Expression Regulation, Neoplastic, MicroRNAs, Cell culture, Drug Resistance, Neoplasm, miR-182, Cancer research, Apoptosis Regulatory Proteins, medicine.drug
Abstract

Background Overexpression of microRNA-182 (miR-182) is found in various human cancers, including non-small cell lung cancer (NSCLC). Our aim is to investigate the association of miR-182 expression with the sensitivity of NSCLC to cisplatin. Methods TaqMan RT-PCR or Western blot assay was performed to detect the expression of mature miR-182 and programmed cell death 4 (PDCD4) protein. miR-182 and (or) PDCD4 depleted cell lines were generated using miR-182 inhibitor and (or) siRNA. The viabilities of treated cells were analyzed using MTT assay. Results The expression level of miR-182 in A549 cell line was significantly higher than that in NHBE cell line (p

Published
2014

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