236 results on '"Lei Chen"'
Search Results
2. The efficacy and safety of patent Foramen Ovale Closure for Refractory Epilepsy (PFOC-RE): a prospectively randomized control trial of an innovative surgical therapy for refractory epilepsy patients with PFO of high-grade right-to-left shunt
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Shuming, Ji, Hua, Li, Yusha, Tang, and Lei, Chen
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- 2023
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3. Comparison of alveolar bone width and sagittal tooth angulation of maxillary central incisors in Class I and Class III canine relationships: a retrospective study using CBCT
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Lei, Chen, Yu, Qun, Wu, Di, Cai, Kunzhan, Weigl, Paul, and Tang, Chunbo
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- 2022
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4. Evaluation of droplet digital PCR rapid detection method and precise diagnosis and treatment for suspected sepsis (PROGRESS): a study protocol for a multi-center pragmatic randomized controlled trial
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Zhao, Yuanhan, Lin, Ke, Zhang, Haocheng, Yuan, Guanmin, Zhang, Yanliang, Pan, Jingye, Hong, Liang, Huang, Yan, Ye, Ying, Huang, Lisu, Chen, Xiaohua, Liu, Jun, Li, Xiang, He, Xiaoju, Yue, Qiaoyan, Zhang, Hong, Zhou, Aiming, Zhuang, Yangyang, Chen, Jie, Wu, Caixia, Zhou, Wei, Cai, Fujing, Zhang, Shengguo, Li, Liang, Li, Shaling, Bian, Tingting, Li, Jiabin, Yin, Jun, Ruan, Zhengshang, Xu, Shanshan, Zhang, Yan, Chen, Jie, Zhang, Ying, Han, Jun, Su, Tingting, Tu, Fan, Jiang, Lijing, Lei, Chen, Du, Qiu, Ai, Jingwen, and Zhang, Wenhong
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- 2022
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5. Frailty index is useful for predicting postoperative morbidity in older patients undergoing gastrointestinal surgery: a prospective cohort study
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Gu, Chaoyang, Lu, Anqing, Lei, Chen, Wu, Qingbin, Zhang, Xubing, Wei, Mingtian, and Wang, Ziqiang
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- 2022
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6. A predictive model of macrosomic birth based upon real-world clinical data from pregnant women
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Jing, Gao, Huwei, Shi, Chao, Chen, Lei, Chen, Ping, Wang, Zhongzhou, Xiao, Sen, Yang, Jiayuan, Chen, Ruiyao, Chen, Lu, Lu, Shuqing, Luo, Kaixiang, Yang, Jie, Xu, and Weiwei, Cheng
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- 2022
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7. Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects
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Ni, Xuejun, Shan, Xiuying, Xu, Lili, Yu, Wenjun, Zhang, Mingliang, Lei, Chen, Xu, Nating, Lin, Junyu, and Wang, Biao
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- 2021
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8. A bispecific CAR-T cell therapy targeting BCMA and CD38 in relapsed or refractory multiple myeloma
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Huiwen Jiang, Ping Yin, Haiming Kou, Dan Jin, Chunyan Sun, Zhihui Wang, Zhiping Huang, Yu Hu, Lisha Ai, Li Cai, Xinying Zhao, Gaoquan Hua, Chenggong Li, Linghui Xia, Heng Mei, Lei Chen, Jun Deng, Tao Guo, Lin Liu, Lu Tang, Yimeng Xia, and Sha Ke
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Oncology ,Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Chimeric antigen receptor-T cells ,medicine.medical_treatment ,Neutropenia ,CD38 ,Immunotherapy, Adoptive ,Mice ,Antigen ,Multiple myeloma ,Internal medicine ,Cell Line, Tumor ,Medicine ,Animals ,Humans ,Diseases of the blood and blood-forming organs ,B-Cell Maturation Antigen ,Molecular Biology ,RC254-282 ,Aged ,Hematology ,Leukopenia ,Receptors, Chimeric Antigen ,business.industry ,Research ,Bispecific CAR ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Immunotherapy ,Middle Aged ,medicine.disease ,ADP-ribosyl Cyclase 1 ,BCMA ,Molecular Docking Simulation ,Cytokine release syndrome ,Female ,medicine.symptom ,RC633-647.5 ,Neoplasm Recurrence, Local ,business - Abstract
Background BCMA-specific chimeric antigen receptor-T cells (CAR-Ts) have exhibited remarkable efficacy in refractory or relapsed multiple myeloma (RRMM); however, primary resistance and relapse exist with single-target immunotherapy. Bispecific CARs are proposed to mitigate these limitations. Methods We constructed a humanized bispecific BM38 CAR targeting BCMA and CD38 and tested the antimyeloma activity of BM38 CAR-Ts in vitro and in vivo. Twenty-three patients with RRMM received infusions of BM38 CAR-Ts in a phase I trial. Results BM38 CAR-Ts showed stronger in vitro cytotoxicity to heterogeneous MM cells than did T cells expressing an individual BCMA or CD38 CAR. BM38 CAR-Ts also exhibited potent antimyeloma activity in xenograft mouse models. In the phase I trial, cytokine release syndrome occurred in 20 patients (87%) and was mostly grade 1–2 (65%). Neurotoxicity was not observed. Hematologic toxicities were common, including neutropenia in 96% of the patients, leukopenia in 87%, anemia in 43% and thrombocytopenia in 61%. At a median follow-up of 9.0 months (range 0.5 to 18.5), 20 patients (87%) attained a clinical response and minimal residual disease-negativity (≤ 10–4 nucleated cells), with 12 (52%) achieving a stringent complete response. Extramedullary plasmacytoma was eliminated completely in 56% and partially in 33% and of 9 patients. The median progression-free survival was 17.2 months. Two relapsed patients maintained BCMA and CD38 expression on MM cells. Notably, BM38 CAR-Ts cells were detectable in 77.8% of evaluable patients at 9 months and 62.2% at 12 months. Conclusion Bispecific BM38 CAR-Ts were feasible, safe and significantly effective in patient with RRMM. Trial registration: Chictr.org.cn ChiCTR1800018143.
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- 2021
9. Adjuvant chemotherapy is an additional option for locally advanced gastric cancer after radical gastrectomy with D2 lymphadenectomy: a retrospective control study
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Zhendan Yao, Chenghai Zhang, Maoxing Liu, Beihai Jiang, Ming Cui, Jiadi Xing, Xiangqian Su, Fei Tan, Kai Xu, Hong Yang, Yuzhe Li, Lei Chen, and Nan Zhang
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Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Survival ,medicine.medical_treatment ,Adenocarcinoma ,Gastroenterology ,Young Adult ,FOLFOX ,Surgical oncology ,Gastrectomy ,Stomach Neoplasms ,Statistical significance ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,Genetics ,medicine ,Humans ,Stage (cooking) ,RC254-282 ,Aged ,Retrospective Studies ,Chemotherapy ,business.industry ,Cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Middle Aged ,medicine.disease ,Prognosis ,Adjuvant chemotherapy ,Clinical trial ,Survival Rate ,Oncology ,Chemotherapy, Adjuvant ,Case-Control Studies ,Lymph Node Excision ,Female ,business ,Gastric cancer ,Adjuvant ,medicine.drug ,Research Article ,Follow-Up Studies - Abstract
Background This study compared the long-term efficacy of different durations of adjuvant chemotherapy for patients with gastric cancer after radical gastrectomy with D2 lymphadenectomy. Methods We retrospectively identified 428 patients with stage II–III gastric cancer who underwent D2 gastrectomy between 2009 and 2016. Patients were divided into four groups according to the duration of adjuvant chemotherapy, including 0 week (no adjuvant, group A), 20 to 24 weeks (completed 7–8 cycles every 3 weeks or 10–12 cycles every 2 weeks, group B), and 12 to18 weeks (completed 4–6 cycles every 3 weeks or 6–9 cycles every 2 weeks, group C), and less than 12 weeks (received up to 3 cycles every 3 weeks or 5 cycles every 2 weeks, group D). The chemotherapy regimens included XELOX, SOX, and FOLFOX. 5-year overall survival (OS) and disease-free survival (DFS) were analyzed. Results The 5-year OS rates for groups A, B, C, and D were 52.3, 73.7, 72.0, and 53.3%, respectively, and the 5-year DFS rates were 50.0, 68.0, 65.4, and 50.0%, respectively. OS and DFS were higher in group B than in groups A and D. Similarly, patients in group C were more likely to have higher OS and DFS than those in groups A and D. Meanwhile, there were no significant differences in OS and DFS between groups B and C. The multivariate analysis confirmed with high statistical significance the efficacy of complete courses of adjuvant chemotherapy, and, among them, the similar impact of 4–6/6–9 and 7–8/10–12 cycles, resulting in similar HRs vs Group A (0.52 and 0.42, respectively). Conclusions To reduce toxicity and maintain efficacy, XELOX or SOX chemotherapy regimens administered for 4–6 cycles every 3 weeks or FOLFOX regimen for 6–9 cycles every 2 weeks might be a favorable option for patients with stage II–III gastric cancer after D2 gastrectomy. Prospective multicenter clinical trials with adequate sample sizes are necessary to verify these findings.
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- 2021
10. The impacts of Chinese drug volume-based procurement policy on the use of policy-related antibiotic drugs in Shenzhen, 2018–2019: an interrupted time-series analysis
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Bo Zheng, Ying Yang, Lei Chen, Zongfu Mao, and Xinfeng Ke
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medicine.medical_specialty ,China ,volume-based procurement ,"4 + 7" ,030226 pharmacology & pharmacy ,Interrupted Time Series Analysis ,Health administration ,antibiotic use ,03 medical and health sciences ,0302 clinical medicine ,Procurement ,Environmental health ,Medicine ,Humans ,Government ,National Centralized Drug Procurement (NCDP) policy ,business.industry ,030503 health policy & services ,Health Policy ,Nursing research ,Public health ,Research ,Bidding ,Anti-Bacterial Agents ,Pharmaceutical Preparations ,Public aspects of medicine ,RA1-1270 ,Health Expenditures ,0305 other medical science ,business ,Cefuroxime ,medicine.drug - Abstract
Background In 2019, Chinese government implemented volume-based procurement of 25 drugs in 4 municipalities and 7 sub-provincial cities, i.e. “4 + 7” policy. Competitive bidding was conducted by the government based on the annual agreed procurement volume submitted by each public medical institution in pilot cities. Pilot cities were required to implement bid winning results in March 2019 and the use volume of bid winning products was examined to ensure the completion of agreed procurement volume. In the policy, an oral antibiotic (cefuroxime) was included. Given the current condition of the irrational use of antibiotics in China, this study aims to evaluate the impact of “4 + 7” policy on the use of policy-related antibiotics. Methods This study used drug purchase data from the Centralized Drug Procurement Survey in Shenzhen 2019, covering 24 months from January 2018 to December 2019. Oral antibiotic drugs related to “4 + 7” policy were selected as study samples, including cefuroxime and 12 antibiotic drugs that have an alternative relationship with cefuroxime in clinical use. Purchase volume and expenditures were selected as outcome variables, and were measured using Defined Daily Doses (DDDs) and Chinese yuan, respectively. Segmented linear regression analysis with interrupted time series was adopted to examine the effect of “4 + 7” policy. Results After the implementation of “4 + 7” policy, the overall volume of cefuroxime and its alternative drugs increased from 9.47 million DDDs to 13.42 million DDDs, with an increase of 41.8 %. The results of segmented linear regression showed that the volume of cefuroxime significantly increased 161.16 thousand DDDs after “4 + 7” policy (95 % CI: 59.43 to 262.90, p-value = 0.004). The volume of alternative drugs significantly increased 273.65 thousand DDDs (95 % CI: 90.17 to 457.12, p-value = 0.006). The overall “4 + 7” policy-related antibiotics significantly increased 436.31 thousand DDDs (95 % CI: 190.81 to 681.81, p-value = 0.001) after “4 + 7” policy. Conclusions This study provides evidence that the implementation of “4 + 7” volume-based procurement policy was associated with significant increases in the volume of policy-related antibiotic drugs. The increase in antibiotic use after the policy needs special attention and vigilance.
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- 2021
11. Protective effects of sodium butyrate on rotavirus inducing endoplasmic reticulum stress-mediated apoptosis via PERK-eIF2α signaling pathway in IPEC-J2 cells
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Ye Zhao, Jun Jiang, Manyi Xiong, Linyuan Shen, Ming Zhang, Daiwen Chen, Shunhua Zhang, Ningming Hu, Li Zhu, Lei Chen, Qin Jiang, Lili Niu, Mingxian Yang, and Jiandong Yang
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0301 basic medicine ,Rotavirus ,Glucose-regulated protein ,Veterinary medicine ,Apoptosis ,Biochemistry ,SF1-1100 ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Downregulation and upregulation ,SF600-1100 ,PERK-eIF2α ,biology ,Kinase ,Chemistry ,Endoplasmic reticulum ,Research ,Sodium butyrate ,Cell biology ,Animal culture ,IPEC-J2 ,030104 developmental biology ,030220 oncology & carcinogenesis ,biology.protein ,Unfolded protein response ,Animal Science and Zoology ,Signal transduction ,Food Science ,Biotechnology - Abstract
Background Rotavirus (RV) is a major pathogen that causes severe gastroenteritis in infants and young animals. Endoplasmic reticulum (ER) stress and subsequent apoptosis play pivotal role in virus infection. However, the protective mechanisms of intestinal damage caused by RV are poorly defined, especially the molecular pathways related to enterocytes apoptosis. Thus, the aim of this study was to investigate the protective effect and mechanism of sodium butyrate (SB) on RV-induced apoptosis of IPEC-J2 cells. Results The RV infection led to significant cell apoptosis, increased the expression levels of ER stress (ERS) markers, phosphorylated protein kinase-like ER kinase (PERK), eukaryotic initiation factor 2 alpha (eIF2α), caspase9, and caspase3. Blocking PERK pathway using specific inhibitor GSK subsequently reversed RV-induced cell apoptosis. The SB treatment significantly inhibited RV-induced ERS by decreasing the expression of glucose regulated protein 78 (GRP78), PERK, and eIF2α. In addition, SB treatment restrained the ERS-mediated apoptotic pathway, as indicated by downregulation of C/EBP homologous protein (CHOP) mRNA level, as well as decreased cleaved caspase9 and caspase3 protein levels. Furthermore, siRNA-induced GPR109a knockdown significantly suppressed the protective effect of SB on RV-induced cell apoptosis. Conclusions These results indicate that SB exerts protective effects against RV-induced cell apoptosis through inhibiting ERS mediated apoptosis by regulating PERK-eIF2α signaling pathway via GPR109a, which provide new ideas for the prevention and control of RV.
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- 2021
12. Circular RNA circSDHC serves as a sponge for miR-127-3p to promote the proliferation and metastasis of renal cell carcinoma via the CDKN3/E2F1 axis
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Jiaxing Zhang, Dan-Lei Chen, Xiaozhong Liao, Wei Chen, Mi Zhou, Junjie Cen, Yi-Hui Pan, Guan-Nan Shu, Yanping Liang, Zhousan Zheng, Yong Huang, Yong Fang, and Junhang Luo
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0301 basic medicine ,Male ,Cancer Research ,Microarray ,Carcinogenesis ,urologic and male genital diseases ,Metastasis ,0302 clinical medicine ,Renal cell carcinoma ,E2F1 ,Neoplasm Metastasis ,Cyclin-Dependent Kinase Inhibitor Proteins ,Gene knockdown ,Mice, Inbred BALB C ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Kidney Neoplasms ,female genital diseases and pregnancy complications ,Gene Expression Regulation, Neoplastic ,Oncology ,030220 oncology & carcinogenesis ,miR-127-3p ,Disease Progression ,Molecular Medicine ,Dual-Specificity Phosphatases ,Female ,Signal Transduction ,Down-Regulation ,Mice, Nude ,In situ hybridization ,Biology ,lcsh:RC254-282 ,E2F1 pathway ,03 medical and health sciences ,In vivo ,Circular RNA ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Neoplasm Invasiveness ,Carcinoma, Renal Cell ,Cell Proliferation ,Proportional Hazards Models ,Base Sequence ,Research ,RNA, Circular ,medicine.disease ,MicroRNAs ,030104 developmental biology ,Multivariate Analysis ,Cancer research ,circSDHC ,E2F1 Transcription Factor - Abstract
Background There is increasing evidence that circular RNAs (circRNAs) have significant regulatory roles in cancer development and progression; however, the expression patterns and biological functions of circRNAs in renal cell carcinoma (RCC) remain largely elusive. Method Bioinformatics methods were applied to screen for circRNAs differentially expressed in RCC. Analysis of online circRNAs microarray datasets and our own patient cohort indicated that circSDHC (hsa_circ_0015004) had a potential oncogenic role in RCC. Subsequently, circSDHC expression was measured in RCC tissues and cell lines by qPCR assay, and the prognostic value of circSDHC evaluated. Further, a series of functional in vitro and in vivo experiments were conducted to assess the effects of circSDHC on RCC proliferation and metastasis. RNA pull-down assay, luciferase reporter and fluorescent in situ hybridization assays were used to confirm the interactions between circSDHC, miR-127-3p and its target genes. Results Clinically, high circSDHC expression was correlated with advanced TNM stage and poor survival in patients with RCC. Further, circSDHC promoted tumor cell proliferation and invasion, both in vivo and in vitro. Analysis of the mechanism underlying the effects of circSDHC in RCC demonstrated that it binds competitively to miR-127-3p and prevents its suppression of a downstream gene, CDKN3, and the E2F1 pathway, thereby leading to RCC malignant progression. Furthermore, knockdown of circSDHC caused decreased CDKN3 expression and E2F1 pathway inhibition, which could be rescued by treatment with an miR-127-3p inhibitor. Conclusion Our data indicates, for the first time, an essential role for the circSDHC/miR-127-3p/CDKN3/E2F1 axis in RCC progression. Thus, circSDHC has potential to be a new therapeutic target in patients with RCC.
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- 2021
13. Astaxanthin protects cognitive function of vascular dementia
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Xiao Liang, Yajun Zhi, Jialing Zou, Ming Zhang, Xiaokun Yao, Guizhen Ying, Lei Chen, Xiaolan Yin, Hongwei Li, Ningwei Zhu, and Hui Yang
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Male ,Cognition function ,Interleukin-1beta ,Morris water navigation task ,Hippocampal formation ,Xanthophylls ,medicine.disease_cause ,Vascular dementia ,Hippocampus ,lcsh:RC346-429 ,Anti-oxidant ,Behavioral Neuroscience ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Discrimination, Psychological ,Morris Water Maze Test ,Malondialdehyde ,Bicinchoninic acid assay ,Hippocampus (mythology) ,0303 health sciences ,Mice, Inbred ICR ,biology ,General Medicine ,Neuroprotective Agents ,medicine.medical_specialty ,Cognitive Neuroscience ,Prefrontal Cortex ,Superoxide dismutase ,03 medical and health sciences ,Internal medicine ,Anti-inflammation ,medicine ,Animals ,lcsh:Neurology. Diseases of the nervous system ,Biological Psychiatry ,030304 developmental biology ,business.industry ,Superoxide Dismutase ,Research ,Dementia, Vascular ,Astaxanthin ,Recognition, Psychology ,medicine.disease ,Endocrinology ,chemistry ,biology.protein ,Interleukin-4 ,business ,Cognition Disorders ,030217 neurology & neurosurgery ,Oxidative stress ,Psychomotor Performance - Abstract
Objective The purpose of this study was to evaluate the effect of astaxanthin (AST) on cognition function, inflammatory response and oxidative stress in vascular dementia (VD) mice. Method VD mice model was established by left unilateral common carotid arteries occlusion (LUCCAO). Following LUCCAO, AST was intragastrically administered for 30 days. Object recognition test and morris water maze test were used to evaluate cognitive function. Hematoxylin and eosin staining was performed to observe the hippocampal neuron structure. Enzyme-linked immunosorbent assay kit and bicinchoninic acid kit were respectively adopted to measure IL-1β and IL-4 protein expression and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in hippocampus and prefrontal cortex. Results AST improved the discrimination ability of VD mice. The escape latency and path length of VD mice treated with AST were dramatically reduced. Besides, AST 200 mg/kg enhanced crossing platform time and the number of times crossing the platform quadrant, and alleviated the morphological impairment in VD mice. Moreover, we found that AST inhibited IL-1β expression and MDA content, whereas promoted IL-4 expression and SOD activity in a dose-dependent manner. Conclusion AST could improve cognitive impairment and hippocampal neurons in VD mice, which may be related to suppression of inflammatory response and oxidative stress.
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- 2020
14. Cross-feeding between cyanobacterium Synechococcus and Escherichia coli in an artifcial autotrophic–heterotrophic coculture system revealed by integrated omics analysis.
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Jiajia Ma, Taohong Guo, Meijin Ren, Lei Chen, Xinyu Song, and Weiwen Zhang
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AUTOTROPHIC bacteria ,SYNECHOCOCCUS ,ESCHERICHIA coli ,SYNTROPHISM ,AXENIC cultures ,SUSTAINABILITY ,METABOLOMICS ,CELL culture - Abstract
Background: Light-driven consortia, which consist of sucrose-secreting cyanobacteria and heterotrophic species, have attracted considerable attention due to their capability for the sustainable production of valuable chemicals directly from CO
2 . In a previous study, we achieved a one-step conversion of sucrose secreted from cyanobacteria to fne chemicals by constructing an artifcial coculture system consisting of sucrose-secreting Synechococcus elongateus cscB+ and 3-hydroxypropionic acid (3-HP) producing Escherichia coli ABKm. Analyses of the coculture system showed that the cyanobacterial cells grew better than their corresponding axenic cultures. To explore the underlying mechanism and to identify the metabolic nodes with the potential to further improve the coculture system, we conducted integrated transcriptomic, proteomic and metabolomic analyses. Results: We frst explored how the relieved oxidative stress afected cyanobacterial cell growth in a coculture system by supplementing additional ascorbic acid to CoBG-11 medium. We found that the cell growth of cyanobacteria was clearly improved with an additional 1 mM ascorbic acid under axenic culture; however, its growth was still slower than that in the coculture system, suggesting that the improved growth of Synechococcus cscB+ may be caused by multiple factors, including reduced oxidative stress. To further explore the cellular responses of cyanobacteria in the system, quantitative transcriptomics, proteomics and metabolomics were applied to Synechococcus cscB+ . Analyses of diferentially regulated genes/proteins and the abundance change of metabolites in the photosystems revealed that the photosynthesis of the cocultured Synechococcus cscB+ was enhanced. The decreased expression of the CO2 transporter suggested that the heterotrophic partner in the system might supplement additional CO2 to support the cell growth of Synechococcus cscB+ . In addition, the diferentially regulated genes and proteins involved in the nitrogen and phosphate assimilation pathways suggested that the supply of phosphate and nitrogen in the Co-BG11 medium might be insufcient. Conclusion: An artifcial coculture system capable of converting CO2 to fne chemicals was established and then analysed by integrated omics analysis, which demonstrated that in the coculture system, the relieved oxidative stress and increased CO2 availability improved the cell growth of cyanobacteria. In addition, the results also showed that the supply of phosphate and nitrogen in the Co-BG11 medium might be insufcient, which paves a new path towards the optimization of the coculture system in the future. Taken together, these results from the multiple omics analyses provide strong evidence that benefcial interactions can be achieved from cross-feeding and competition between phototrophs and prokaryotic heterotrophs and new guidelines for engineering more intelligent artifcial consortia in the future. [ABSTRACT FROM AUTHOR]- Published
- 2022
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15. The influence of non-clinical pharmacists' understanding of and attitudes towards pharmaceutical care on their willingness to serve as clinical pharmacists in China.
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Chuchuan Wan, Yuankai Huang, Lei Chen, Xiaoyu Xi, Wan, Chuchuan, Huang, Yuankai, Chen, Lei, and Xi, Xiaoyu
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PHARMACISTS' attitudes ,PHARMACIST-patient relationships ,PHARMACISTS ,LOGISTIC regression analysis ,MARITAL status - Abstract
Background: The shortage of clinical pharmacists is severe in China, and transferring non-clinical pharmacists into clinical pharmacists serves as a feasible solution to this problem. In China, a one-year training programme is available for non-clinical pharmacists, and those who have finished the programme are certificated as clinical pharmacists. However, not all non-clinical pharmacists are willing to serve as clinical pharmacists, and their willingness to serve as clinical pharmacists may be related to their attitudes towards pharmaceutical care. This study aims to test whether non-clinical pharmacists' attitudes towards pharmaceutical care is positively correlated with their willingness to serve as clinical pharmacists.Methods: A cross-sectional survey was conducted in secondary and tertiary hospitals in China to collect non-clinical pharmacists' basic demographic information, attitudes towards pharmaceutical care and willingness to serve as clinical pharmacists. An ordered logistic regression analysis was performed to test the relationship between non-clinical pharmacists' attitudes towards pharmaceutical care and their willingness to serve as clinical pharmacists.Results: One thousand five hundred eighty non-clinical pharmacists from 755 hospitals were invited to participate in the study. Finally, 1308 valid responses were obtained and the response rate reached 82.78%. The regression results (R2 = 0.052, chi-square = 174.024, p < 0.0001) suggested that attitudes towards pharmaceutical care had a positive impact on non-clinical pharmacists' willingness to serve as clinical pharmacists (p < 0.01). Control variables, such as age (p < 0.05), marital status (p < 0.05), professional title (p < 0.1), educational level (p < 0.1), salary expectations (p < 0.01) and experience providing pharmaceutical care (p < 0.01), also influenced non-clinical pharmacists' willingness to serve as clinical pharmacists.Conclusions: Based on the results, suggestions are made to increase non-clinical pharmacists' willingness to serve as clinical pharmacists. [ABSTRACT FROM AUTHOR]- Published
- 2022
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16. Maternal oxygen exposure may not change umbilical cord venous partial pressure of oxygen: non-random, paired venous and arterial samples from a randomised controlled trial
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Wen Jiang, Xiaobin Xu, Aiming Wang, Yunhai Chuai, Lei Chen, and Yuanqing Yao
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Adult ,Percentile ,Partial Pressure ,lcsh:Gynecology and obstetrics ,Umbilical cord ,law.invention ,Veins ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,Interquartile range ,Labor Stage, Second ,Pregnancy ,medicine ,Humans ,030212 general & internal medicine ,Umbilical cord venous PO2 ,Intrauterine Resuscitation ,lcsh:RG1-991 ,030219 obstetrics & reproductive medicine ,business.industry ,Oxygen Inhalation Therapy ,Obstetrics and Gynecology ,The second stage of labor ,Arteries ,Fetal Blood ,Low flow oxygen ,Oxygen ,medicine.anatomical_structure ,Anesthesia ,Room air distribution ,Arterial blood ,Female ,Hemoglobin ,business ,Research Article - Abstract
Background Despite the widespread use of oxygen (O2) in intrauterine resuscitation, the obstetric scientists’ understanding of O2 therapy is full of contradictions. We tested the hypothesis that higher maternal arterial partial pressure of oxygen (PO2) is associated with higher umbilical cord venous PO2 (UvPO2). Methods This is a planned secondary analysis of a randomised controlled trial (RCT), 443 normal women were 1:1 randomly allocated to receive 2 L/min O2 or room air from the onset of second stage to delivery. We reported that maternal 2 L/min O2 exposure cannot affect the umbilical cord arterial pH or the fetal heart rate (FHR) pattern. In 217 non-random samples, we found 2 L/min O2 exposure increased the maternal arterial PO2 to the median 150 mmHg (hemoglobin would be saturated). The primary outcome for this analysis was UvPO2 in these non-random samples. Results There were no significant differences between the O2 group (N = 107) and the control group (N = 110) in the UvPO2 (median 30.2, interquartile 25.4–35.2 versus median 28.3, interquartile 23.4–35.3, mmHg, P = 0.379). There were also no significant differences between room air and different percentiles of O2 exposure duration (< 25th, ≧ 25th 2. Conclusions Maternal O2 exposure at super-physiological levels (median arterial blood PO2 150 mmHg) in normal labor may not change the UvPO2. Clinical trial registration ClinicalTrials.govNCT02221440, first posted in 20 August 2014.
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- 2020
17. Association between diet-related knowledge, attitudes, behaviors, and self-rated health in Chinese adult residents: a population-based study
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Liuyi Wei, Ying Yang, Mi Luo, Zongfu Mao, Di He, Shizhen Wang, and Lei Chen
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Adult ,Male ,Rural Population ,medicine.medical_specialty ,China ,Health Knowledge, Attitudes, Practice ,Adolescent ,Urban Population ,030309 nutrition & dietetics ,media_common.quotation_subject ,Literacy ,Nutrition Policy ,03 medical and health sciences ,Diagnostic Self Evaluation ,Young Adult ,0302 clinical medicine ,Self-rated health ,Asian People ,Environmental health ,Epidemiology ,Medicine ,Humans ,030212 general & internal medicine ,Association (psychology) ,media_common ,Aged ,0303 health sciences ,business.industry ,lcsh:Public aspects of medicine ,Public health ,Public Health, Environmental and Occupational Health ,lcsh:RA1-1270 ,Feeding Behavior ,Middle Aged ,Nutrition Surveys ,Test (assessment) ,Diet ,Knowledge ,Attitudes ,Behaviors ,Female ,Ordered logit ,Biostatistics ,Diet, Healthy ,business ,Research Article - Abstract
Background Diet-related knowledge, attitudes, and behaviors (KABs) are important for building healthier dietary patterns. We conducted this study to (a) investigate diet conditions of Chinese adult residents from the perspective of knowledge, attitudes, and behaviors, and (b) assess the association between diet-related KABs and self-rated health. Methods We analyzed the 2015 China Health and Nutrition Survey (CHNS) data. Individuals aged 18 years and older were included as study subjects (n = 12,814), assessing their diet-related knowledge, attitudes, behaviors, and self-rated health. Comparison of diet-related KABs in urban and rural residents was conducted using chi-square test. Ordinal logistic regression analysis was adopted to examine the association between diet-related KABs and self-rated health. Results The proportion of knowing about the Chinese Food Pagoda (CFP) or the Dietary Guidelines for Chinese Residents (DGCR) was 27.1%. 34.3% of the participants were assessed as having adequate dietary knowledge literacy. 24.3% reported a positive attitude towards healthy eating. 27.6 and 65.9% of the participants reported proactively looking for nutrition knowledge and preferring eating fruits & vegetables, respectively. Chi-square test indicated that rural people experienced poorer diet-related knowledge, attitudes, and behaviors than urban residents (all p-values OR = 1.11, 95% CI = 1.08–1.15), had adequate dietary knowledge literacy (OR = 1.12, 95% CI = 1.10–1.15), held positive attitude towards healthy eating (OR = 1.14, 95% CI = 1.09–1.19), proactively looked for nutrition knowledge (OR = 1.11, 95% CI = 1.08–1.15), and preferred eating fruits & vegetables (OR = 1.09, 95% CI = 1.07–1.12) had significantly better self-rated health. Conclusions Chinese adult residents experienced poor diet-related knowledge, attitudes, and behaviors. Rural people had significantly worse diet conditions than urban residents. Better diet-related knowledge, attitudes, and behaviors were associated with higher self-rated health in Chinese adult residents.
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- 2020
18. Successful implementation of an enhanced recovery after surgery (ERAS) protocol reduces nausea and vomiting after infratentorial craniotomy for tumour resection: a randomized controlled trial
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Zhengmin Li, Dan Lu, Xude Sun, Long Chen, Shiming He, Jiangtao Niu, Yuan Wang, Tianzhi Zhao, Yufu Zhang, Yafei Xue, Binfang Zhao, Tao Zheng, Wenhai Lv, Mingjuan Li, Lanfu Zhao, Lei Chen, Lin Ye, Bo Chen, Guodong Gao, Lin Ma, and Bolin Liu
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medicine.medical_specialty ,Nausea ,medicine.drug_class ,medicine.medical_treatment ,Anxiety ,lcsh:RC346-429 ,law.invention ,Enhanced recovery after surgery (ERAS) ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,medicine ,Antiemetic ,Humans ,Cumulative incidence ,lcsh:Neurology. Diseases of the nervous system ,Craniotomy ,business.industry ,Brain Neoplasms ,General Medicine ,Sleep quality ,Surgery ,Postoperative nausea and vomiting (PONV) ,Infratentorial craniotomy ,Retraction Note ,030220 oncology & carcinogenesis ,Postoperative Nausea and Vomiting ,Vomiting ,Neurology (clinical) ,Neurosurgery ,medicine.symptom ,business ,Enhanced Recovery After Surgery ,030217 neurology & neurosurgery ,Postoperative nausea and vomiting ,Research Article - Abstract
Background Infratentorial craniotomy patients have a high incidence of postoperative nausea and vomiting (PONV). Enhanced Recovery After Surgery (ERAS) protocols have been shown in multiple surgical disciplines to improve outcomes, including reduced PONV. However, very few studies have described the application of ERAS to infratentorial craniotomy. The aim of this study was to examine whether our ERAS protocol for infratentorial craniotomy could improve PONV. Methods We implemented an evidence-based, multimodal ERAS protocol for patients undergoing infratentorial craniotomy. A total of 105 patients who underwent infratentorial craniotomy were randomized into either the ERAS group (n = 50) or the control group (n = 55). Primary outcomes were the incidence of vomiting, nausea score, and use of rescue antiemetic during the first 72 h after surgery. Secondary outcomes included postoperative anxiety level, sleep quality, and complications. Results Over the entire 72 h post-craniotomy observation period, the cumulative incidence of vomiting was significantly lower in the ERAS group than in the control group. Meanwhile, the incidence of vomiting was significantly lower in the ERAS group on postoperative days (PODs) 2 and 3. Notably, the proportion of patients with mild nausea (VAS 0–4) was higher in the ERAS group as compared to the control group on PODs 2 or 3. Additionally, the postoperative anxiety level and quality of sleep were significantly better in the ERAS group. Conclusion Successful implementation of our ERAS protocol in infratentorial craniotomy patients could attenuate postoperative anxiety, improve sleep quality, and reduce the incidence of PONV, without increasing the rate of postoperative complications. Trial registration ChiCTR-INR-16009662, 27 Oct 2016, Clinical study on the development and efficacy evaluation of Enhanced Recovery After Surgery (ERAS) in Neurosurgery.
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- 2020
19. The protective effects of PI3K/Akt pathway on human nucleus pulposus mesenchymal stem cells against hypoxia and nutrition deficiency
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Dasheng Tian, Lei Chen, Jianjun Liu, Juehua Jing, and Bin Zhu
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0301 basic medicine ,lcsh:Diseases of the musculoskeletal system ,Nucleus Pulposus ,medicine.medical_treatment ,Primary Cell Culture ,Intervertebral Disc Degeneration ,Cell therapy ,03 medical and health sciences ,Phosphatidylinositol 3-Kinases ,0302 clinical medicine ,lcsh:Orthopedic surgery ,Human nucleus pulposus-derived mesenchymal stem cells ,Medicine ,Humans ,Orthopedics and Sports Medicine ,Viability assay ,Insulin-Like Growth Factor I ,Hypoxia ,Protein kinase B ,PI3K/AKT/mTOR pathway ,PI3K/Akt ,business.industry ,Caspase 3 ,Growth factor ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Hypoxia (medical) ,Cell biology ,lcsh:RD701-811 ,030104 developmental biology ,Apoptosis ,030220 oncology & carcinogenesis ,Hypoxia and nutrition deficiency ,Surgery ,medicine.symptom ,lcsh:RC925-935 ,business ,Deficiency Diseases ,Proto-Oncogene Proteins c-akt ,Research Article - Abstract
Background To study the effects of hypoxia and nutrition deficiency mimicking degenerated intervertebral disc on the biological behavior of human nucleus-derived pulposus mesenchymal stem cells (hNP-MSCs) and the role of PI3K/Akt pathway in the process in vitro. Methods hP-MSCs were isolated from lumbar disc and were further identified by their immunophenotypes and multilineage differentiation. Then, cells were divided into the control group, hypoxia and nutrition deficiency group, the LY294002 group, and insulin-like growth factor 1 (IGF-1) group. Then cell apoptosis, the cell viability, the caspase 3 activity, and the expression of PI3K, Akt, and functional genes (aggrecan, collagen I, and collagen II) were evaluated. Result Our work showed that isolated cells met the criteria of International Society for cellular Therapy. Therefore, cells obtained from degenerated nucleus pulposus were definitely hNP-MSCs. Our results showed that hypoxia and nutrition deficiency could significantly increase cell apoptosis, the caspase 3 activity, and inhibit cell viability. Gene expression results demonstrated that hypoxia and nutrition deficiency could increase the relative expression of PI3K and Akt gene and inhibit the expression of functional genes. However, when the PI3K/Akt pathway was inhibited by LY294002, the cell apoptosis and caspase 3 activity significantly increased while the cell viability was obviously inhibited. Quantitative real-time PCR results showed that the expression of functional genes was more significantly inhibited. Our study further verified that the above-mentioned biological activities of hNP-MSCs could be significantly improved by IGF1. Conclusions PI3K/Akt signal pathway may have protective effects on human nucleus pulposus-derived mesenchymal stem cells against hypoxia and nutrition deficiency.
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- 2020
20. DHEA Attenuates Microglial Activation via Induction of JMJD3 in Experimental Subarachnoid Haemorrhage
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Wei Li, Han Wang, Yong-Yue Gao, Guang-Jie Liu, Chun-Lei Chen, Yue Lu, Zong Zhuang, Yan Zhou, Xiang-Sheng Zhang, Tao Tao, Ling-Yun Wu, Xuan Shi, and Chun-Hua Hang
- Subjects
Male ,medicine.medical_specialty ,endocrine system ,Jumonji Domain-Containing Histone Demethylases ,medicine.medical_treatment ,Immunology ,Central nervous system ,Dehydroepiandrosterone ,JMJD3 ,Inflammation ,Tropomyosin receptor kinase A ,Neuroprotection ,lcsh:RC346-429 ,Proinflammatory cytokine ,Cellular and Molecular Neuroscience ,Mice ,Internal medicine ,polycyclic compounds ,Medicine ,Animals ,skin and connective tissue diseases ,lcsh:Neurology. Diseases of the nervous system ,Cells, Cultured ,Subarachnoid haemorrhage ,Neurons ,Microglia ,business.industry ,General Neuroscience ,Research ,Brain ,Subarachnoid Hemorrhage ,Steroid hormone ,Disease Models, Animal ,medicine.anatomical_structure ,Endocrinology ,Neuroprotective Agents ,Neurology ,medicine.symptom ,business ,human activities ,hormones, hormone substitutes, and hormone antagonists ,Signal Transduction - Abstract
Background Microglia are resident immune cells in the central nervous system and central to the innate immune system. Excessive activation of microglia after subarachnoid haemorrhage (SAH) contributes greatly to early brain injury, which is responsible for poor outcomes. Dehydroepiandrosterone (DHEA), a steroid hormone enriched in the brain, has recently been found to regulate microglial activation. The purpose of this study was to address the role of DHEA in SAH. Methods We used in vivo models of endovascular perforation and in vitro models of haemoglobin exposure to illustrate the effects of DHEA on microglia in SAH. Results In experimental SAH mice, exogenous DHEA administration increased DHEA levels in the brain and modulated microglial activation. Ameliorated neuronal damage and improved neurological outcomes were also observed in the SAH mice pretreated with DHEA, suggesting neuronal protective effects of DHEA. In cultured microglia, DHEA elevated the mRNA and protein levels of Jumonji d3 (JMJD3, histone 3 demethylase) after haemoglobin exposure, downregulated the H3K27me3 level, and inhibited the transcription of proinflammatory genes. The devastating proinflammatory microglia-mediated effects on primary neurons were also attenuated by DHEA; however, specific inhibition of JMJD3 abolished the protective effects of DHEA. We next verified that DHEA-induced JMJD3 expression, at least in part, through the tropomyosin-related kinase A (TrkA)/Akt signalling pathway. Conclusions DHEA has a neuroprotective effect after SAH. Moreover, DHEA increases microglial JMJD3 expression to regulate proinflammatory/anti-inflammatory microglial activation after haemoglobin exposure, thereby suppressing inflammation.
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- 2019
21. Simulated weightlessness procedure, head-down bed rest impairs adult neurogenesis in the hippocampus of rhesus macaque
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Juan Fu, Shuai Wang, Xiao-Ping Chen, Jinjing Song, Xixia Chu, Lei Chen, Guang He, Xu Zhang, Guanghan Kan, Weizhong Jiang, and Weidong Li
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0301 basic medicine ,Aging ,medicine.medical_treatment ,Neurogenesis ,Hippocampus ,Bed rest ,Adult neurogenesis ,lcsh:RC346-429 ,Micro Report ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,biology.animal ,medicine ,Animals ,Primate ,Molecular Biology ,lcsh:Neurology. Diseases of the nervous system ,Simulated weightlessness ,Weightlessness Simulation ,biology ,Weightlessness ,biology.organism_classification ,Macaca mulatta ,Rhesus macaque ,030104 developmental biology ,Rhesus monkey ,Neuroscience ,030217 neurology & neurosurgery ,Bed Rest - Abstract
The microgravity environment in space can impact astronauts’ cognitive and behavioral activities. However, due to the limitations of research conditions, studies of biological changes in the primate brain, such as neurogenesis, have been comparatively few. We take advantage of − 6° head-down bed rest (HDBR), one of the most implemented space analogue on the ground, to investigate the effects of weightlessness on neurogenesis of non-human primate brain. Rhesus Macaque monkeys were subjected to HDBR for 42 days to simulate weightlessness. BrdU (5-bromodeoxyuridin) and IdU (iododeoxyuridine) were intraperitoneally injected separately before or after HDBR to label the survival and proliferation of newborn neurons. Immunohistochemistry was performed to study the effect of simulated weightlessness on neurogenesis. BrdU staining showed that survival of newborn neurons was reduced, while there were fewer BrdU-positive neurons in the HDBR group compared with the control. Furthermore, IdU-positive neurons also decreased in the HDBR group suggesting a reduced proliferation capacity for these newborn neurons. Our results demonstrate the definite neurogenesis in the adult rhesus macaque hippocampus, and simulated weightlessness HDBR procedure impairs the adult neurogenesis. Electronic supplementary material The online version of this article (10.1186/s13041-019-0459-y) contains supplementary material, which is available to authorized users.
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- 2019
22. Mediastinal follicular dendritic cell sarcoma: a rare, potentially under-recognized, and often misdiagnosed disease
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Xiao-Chu Yan, Yu Zhang, Guangjie Duan, Cheng-Ping Xu, You-Li Wu, Wei Chen, Feng Wu, and Guo-Lei Chen
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0301 basic medicine ,Adult ,Male ,Pathology ,medicine.medical_specialty ,Histology ,Adolescent ,Dendritic Cell Sarcoma, Follicular ,Malignancy ,Pathology and Forensic Medicine ,Metastasis ,03 medical and health sciences ,Pathological diagnosis ,Young Adult ,0302 clinical medicine ,Drug Therapy ,Biopsy ,lcsh:Pathology ,medicine ,Atypia ,Humans ,Aged ,medicine.diagnostic_test ,Radiotherapy ,business.industry ,Research ,Mediastinum ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Lymphoma ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Follicular dendritic cell sarcoma ,Female ,Biopsy, Large-Core Needle ,business ,Radiology ,Tomography, X-Ray Computed ,Epithelioid cell ,lcsh:RB1-214 - Abstract
Background Mediastinal follicular dendritic cell sarcoma (FDCS) is extremely rare. Due to potential under-recognization of this disease, it happens to be misdiagnosed, especially on core needle biopsy. We report 3 cases of mediastinal FDCS and provide a literature review to improve better understanding of the tumor and to reduce misdiagnosis. Methods Three cases of mediastinal FDCS in our clinic practice were studied, including their core needle biopsy and resected specimens, and those cases reported previously in English literature were retrieved and analyzed. Results The core needle biopsy of case 1 showed a tumor reminiscent of classical Hodgkin’s lymphoma (CHL), while the resected mass was finally diagnosed with FDCS combined with hyaline-vascular Castleman’s disease. Both the biopsy and resected tissue of case 2 were constitutive of the clear epithelioid cells with marked atypia. In both cases, definitive diagnoses were not made on core needle biopsy. In case 3, there were some areas morphologically similar to CHL, and some areas contained ovoid to spindle-shaped tumor cells with fascicular pattern. The analysis of 43 cases of mediastinal FDCS showed the age of patients were from 16 to 76 years old, the male to female ratio was 1.5:1, the maximal tumor diameters were 3–17 cm. 18 cases were underwent preoperative biopsy, whereas 15 (83.3%) of which were misdiagnosed initially, often as lymphoma. 32 patients had available follow-up data, the rates of recurrence, metastasis, and mortality were 12.5, 18.8 and 28.1%, respectively. Current limited data suggested no statistical differences between adverse prognosis and gender, age, tumor size, necrosis, or different therapeutics, respectively. Conclusions Mediastinal FDCS is a rare malignancy that has yet not been fully understood and been often misdiagnosed, particularly when making a diagnosis on core needle biopsy. Increased awareness of this enigmatic tumor is crucial to avoid diagnostic pitfalls.
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- 2019
23. ARNTL hypermethylation promotes tumorigenesis and inhibits cisplatin sensitivity by activating CDK5 transcription in nasopharyngeal carcinoma
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Pan Pan Zhang, Xiao-Jing Yang, Na Liu, Xin Wen, Jian Zhang, Hao Peng, Ying Sun, Jun Ma, Qing Mei He, Ying Qin Li, Lei Chen, and Ling Long Tang
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0301 basic medicine ,Cancer Research ,Transcription, Genetic ,CDK5 ,Proliferation ,Mice, Nude ,Chemotherapy sensitivity ,medicine.disease_cause ,lcsh:RC254-282 ,Methylation ,03 medical and health sciences ,Mice ,Random Allocation ,0302 clinical medicine ,Cell Line, Tumor ,medicine ,otorhinolaryngologic diseases ,Nasopharyngeal carcinoma ,Gene silencing ,Animals ,Humans ,Cell Proliferation ,Mice, Inbred BALB C ,Chemistry ,Cell growth ,ARNTL ,Research ,ARNTL Transcription Factors ,Correction ,Cyclin-Dependent Kinase 5 ,Cell cycle ,DNA Methylation ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,stomatognathic diseases ,030104 developmental biology ,HEK293 Cells ,Oncology ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,DNA methylation ,Cancer research ,Female ,Cisplatin ,Carcinogenesis ,Chromatin immunoprecipitation - Abstract
Background Increasing evidence support an important role for DNA methylation in nasopharyngeal carcinoma (NPC). Here, we explored the role of circadian clock gene Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL) methylation in NPC. Methods We employed bisulfite pyrosequencing to determine the epigenetic change of ARNTL in NPC cell lines and tissues. ARNTL mRNA and protein expression in cell lines and tissues were detected by real-time PCR and western blotting. Then, we constructed cell lines overexpressing ARNTL and knocked down ARNTL to explore its function and effect on chemotherapy sensitivity of NPC cell lines to cisplatin in vitro and vivo. Finally, we investigated the potential molecular mechanism of ARNTL by gene set enrichment analysis (GSEA), dual Luciferase reporter assay and chromatin immunoprecipitation assay. Results ARNTL was hypermethylated, and its mRNA and protein were significantly down-regulated in NPC cell lines and tissues. When treated by 5-aza-2′-deoxycytidine, mRNA expression was up-regulated. Overexpression of ARNTL could suppress NPC cells proliferation in vitro and vivo while silencing of ARNTL using shRNA achieved opposite results. GSEA assay found that ARNTL was associated with cell cycle and ectopic ARNTL overexpression could induce G2-M phase arrest. Then, we identified and validated cyclin-dependent kinase 5 (CDK5) as the targeting gene of ARNTL by dual Luciferase reporter assay and chromatin immunoprecipitation assay. When transiently infected ARNTL-overexpression cells with PENTER-vector or PENTER-CDK5 plasmids, the later could reverse the suppressive effects of ARNTL on NPC cell proliferation. Moreover, ARNTL significantly enhanced sensitivity to cisplatin in NPC cells. Conclusions ARNTL suppresses NPC cell proliferation and enhances sensitivity to cisplatin by targeting CDK5. ARNTL may represent a novel therapeutic target for NPC.
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- 2019
24. The genetic locus underlying red foliage and fruit skin traits is mapped to the same location in the two pear bud mutants ‘Red Zaosu’ and ‘Max Red Bartlett’
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Lei Chen, Li Xiugen, Xiaoli Zhang, Zhang Huirong, Yang Jian, Xue Huabai, Wang Long, Wang Suke, Su Yanli, and Jia-Long Yao
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0106 biological sciences ,0301 basic medicine ,lcsh:QH426-470 ,Genetic Linkage ,Locus (genetics) ,Biology ,Genes, Plant ,01 natural sciences ,Pyrus ,03 medical and health sciences ,Genetic linkage ,Genetics ,Cultivar ,Indel ,Indel markers ,Crosses, Genetic ,Hybrid ,Genes, Dominant ,PEAR ,Bulk segregation analysis ,Pigmentation ,Research ,fungi ,food and beverages ,General Medicine ,Plant Leaves ,Horticulture ,lcsh:Genetics ,Plant Breeding ,030104 developmental biology ,Phenotype ,Genetic distance ,red pear ,Fruit ,Shoot ,010606 plant biology & botany - Abstract
Background Red-skinned pears are attractive to consumers because of their aesthetic appeal and the antioxidant-associated health benefits provided by the anthocyanins in their red skin. In China, the ‘Red Zaosu’ (RZS) red bud mutation of the Zaosu (ZS) pear has been used as a parent in Asian pear breeding to generate new cultivars with crispy red fruit and red tender shoots resembling those of the ‘Max Red Bartlett’ (MRB) pears. Results In this study, a segregation ratio of 1:1 was observed between plants with red or green shoots in four families with RZS as the only red shoot gene donor parent, suggesting that the red shoot trait of RZS is associated with a dominant gene. Three markers, In1400–1, In1579–1 and In1579–3, were chosen from 22 pairs of indel primers targeting regions in the vicinity of the previously identified red fruit skin locus of MRB and were able to effectively distinguish the eight red shoot plants from the eight green shoot plants. Linkage analysis indicated that the genetic distance between the two marker loci (In1579–1 and In1579–3) and the red shoot locus of RZS were both 1.4 cM, while the genetic distance between the In1400–1 marker and the red shoot locus was 2.1 cM. The physical position of the red locus in RZS should be in the 368.6 kb candidate interval at the bottom of LG4. Conclusions The genetic locus responsible for the red tender shoots of RZS was located in the same interval of the red fruit skin gene of MRB, meaning that the bud mutation loci of RZS and MRB may be the same or adjacent to each other, and the red shoot trait and the red fruit skin trait in RZS may be controlled by the same, or a closely linked locus. As a result, breeders could use red shoots as a morphological marker to select for the red-skinned hybrids from RZS families. Electronic supplementary material The online version of this article (10.1186/s41065-018-0063-7) contains supplementary material, which is available to authorized users.
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- 2018
25. miR-519a enhances chemosensitivity and promotes autophagy in glioblastoma by targeting STAT3/Bcl2 signaling pathway
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Yuntao Lu, Songtao Qi, Liang Gao, Qiang Zhou, Hong Li, Lei Chen, Junjie Li, Ke Wang, Annie Huang, and Wei-wen Liu
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0301 basic medicine ,STAT3 Transcription Factor ,Cancer Research ,Apoptosis ,Transfection ,lcsh:RC254-282 ,03 medical and health sciences ,Mice ,Nude mouse ,Glioma ,Cell Line, Tumor ,medicine ,Autophagy ,Temozolomide ,Animals ,Humans ,miR-519a ,STAT3 ,Molecular Biology ,Gene knockdown ,Signal transducer and activator of transcription 3 ,biology ,lcsh:RC633-647.5 ,Chemistry ,Research ,lcsh:Diseases of the blood and blood-forming organs ,Hematology ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,biology.organism_classification ,Xenograft Model Antitumor Assays ,nervous system diseases ,MicroRNAs ,030104 developmental biology ,Oncology ,Proto-Oncogene Proteins c-bcl-2 ,Cell culture ,Drug Resistance, Neoplasm ,biology.protein ,Cancer research ,Signal transduction ,Glioblastoma ,Chemoresistance ,Signal Transduction - Abstract
Background Chemoresistance to temozolomide (TMZ) is a major challenge in the treatment of glioblastoma (GBM). We previously found that miR-519a functions as a tumor suppressor in glioma by targeting the signal transducer and activator of transcription 3 (STAT3)-mediated autophagy oncogenic pathway. Here, we investigated the effects of miR-519a on TMZ chemosensitivity and autophagy in GBM cells. Furthermore, the underlying molecular mechanisms and signaling pathways were explored. Methods In the present study, two stable TMZ-resistant GBM cell lines were successfully generated by exposure of parental cells to a gradually increasing TMZ concentration. After transfecting U87-MG/TMZ and U87-MG cells with miR-519a mimic or inhibitor, a series of biochemical assays such as MTT, apoptosis, and colony formation were performed to determine the chemosensitive response to TMZ. The autophagy levels in GBM cells were detected by transmission electron microscopy, LC3B protein immunofluorescence, and Western blotting analysis. Stable knockdown and overexpression of miR-519a in GBM cells were established using lentivirus. A xenograft nude mouse model and in situ brain model were used to examine the in vivo effects of miR-519a. Tumor tissue samples were collected from 48 patients with GBM and were used to assess the relationship between miR-519a and STAT3 expression. Results TMZ treatment significantly upregulated miR-519a in U87-MG cells but not in U87-MG/TMZ cells. Moreover, the expression of miR-519a and baseline autophagy levels was lower in U87-MG/TMZ cells as compared to U87-MG cells. miR-519a dramatically enhanced TMZ-induced autophagy and apoptotic cell death in U87-MG/TMZ cells, while inhibition of miR-519a promoted TMZ resistance and reduced TMZ-induced autophagy in U87-MG cells. Furthermore, miR-519a induced autophagy through modification of STAT3 expression. The in vivo results showed that miR-519a can enhance apoptosis and sensitized GBM to TMZ treatment by promoting autophagy and targeting the STAT3/Bcl-2/Beclin-1 pathway. In human GBM tissues, we found an inverse correlation between miR-519a and STAT3 expression. Conclusions Our results suggested that miR-519a increased the sensitivity of GBM cells to TMZ therapy. The positive effects of miR-519a may be mediated through autophagy. In addition, miR-519a overexpression can induce autophagy by inhibiting STAT3/Bcl-2 pathway. Therefore, a combination of miR-519a and TMZ may represent an effective therapeutic strategy in GBM. Electronic supplementary material The online version of this article (10.1186/s13045-018-0618-0) contains supplementary material, which is available to authorized users.
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- 2018
26. New insight into the mechanism underlying the silk gland biological process by knocking out fibroin heavy chain in the silkworm
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Wen Wang, Yong Cui, Feng Qili, Yongjian Lin, Ya-Nan Zhu, Hui Xiang, and Lei Chen
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0301 basic medicine ,Proteasome Endopeptidase Complex ,lcsh:QH426-470 ,lcsh:Biotechnology ,Mutant ,Silk ,Fibroin ,Biology ,Protein degradation ,medicine.disease_cause ,Transcriptome ,03 medical and health sciences ,Bombyx mori ,lcsh:TP248.13-248.65 ,Genetics ,medicine ,Autophagy ,Animals ,Gene ,CRISPR/Cas9 ,Mutation ,Proteasome ,fungi ,Wild type ,High-Throughput Nucleotide Sequencing ,biology.organism_classification ,Bombyx ,Cell biology ,lcsh:Genetics ,030104 developmental biology ,Insect Proteins ,Bmfib-H ,CRISPR-Cas Systems ,Fibroins ,Biotechnology ,Research Article - Abstract
Background Exploring whether and how mutation of silk protein contributes to subsequent re-allocation of nitrogen, and impacts on the timing of silk gland degradation, is important to understand silk gland biology. Rapid development and wide application of genome editing approach in the silkworm provide us an opportunity to address these issues. Results Using CRISPR/Cas9 system, we successfully performed genome editing of Bmfib-H. The loss-of-function mutations caused naked pupa and thin cocoon mutant phenotypes. Compared with the wild type, the posterior silk gland of mutant showed obviously degraded into fragments in advance of programmed cell death of silk gland cells. Comparative transcriptomic analyses of silk gland at the fourth day of the fifth instar larval stage(L5D4)identified 1456 differential expressed genes (DEGs) between posterior silk gland (PSG) and mid silk gland (MSG) and 1388 DEGs between the mutant and the wild type. Hierarchical clustering of all the DEGs indicated a remarkable down-regulated and an up-regulated gene clade in the mutant silk glands, respectively. Down-regulated genes were overrepresented in the pathways involved in cancer, DNA replication and cell proliferation. Intriguingly, up-regulated DEGs are significantly enriched in the proteasome. By further comparison on the transcriptome of MSG and PSG between the wild type and the mutant, we consistently observed that up-regulated DEGs in the mutant PSG were enriched in protein degrading activity and proteasome. Meantime, we observed a series of up-regulated genes involved in autophagy. Since these protein degradation processes would be normally occur after the spinning time, the results suggesting that these progresses were activated remarkably ahead of schedule in the mutant. Conclusions Accumulation of abnormal fib-H protein might arouse the activation of proteasomes as well as autophagy process, to promote the rapid degradation of such abnormal proteins and the silk gland cells. Our study therefore proposes a subsequent process of protein and partial cellular degradation caused by mutation of silk protein, which might be helpful for understanding its impact of the silk gland biological process, and further exploration the re-allocation of nitrogen in the silkworm. Electronic supplementary material The online version of this article (10.1186/s12864-018-4602-4) contains supplementary material, which is available to authorized users.
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- 2018
27. Anti-EGFR targeted therapy delivered before versus during radiotherapy in locoregionally advanced nasopharyngeal carcinoma: a big-data, intelligence platform-based analysis
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Jun Ma, Yan Ping Mao, Li Tian, Lei Chen, Wen Fei Li, Yuan Zhang, Ying Sun, Ling Long Tang, Hao Peng, Xu Liu, Li Zhi Liu, and Ying Guo
- Subjects
0301 basic medicine ,Oncology ,Male ,Cancer Research ,medicine.medical_treatment ,Cetuximab ,0302 clinical medicine ,Antineoplastic Combined Chemotherapy Protocols ,Medicine ,Molecular Targeted Therapy ,Neoplasm Metastasis ,Chemoradiotherapy ,Induction Chemotherapy ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Prognosis ,ErbB Receptors ,Treatment Outcome ,030220 oncology & carcinogenesis ,Female ,Nimotuzumab ,medicine.drug ,Research Article ,Adult ,medicine.medical_specialty ,Adolescent ,Intensity-modulated radiotherapy ,lcsh:RC254-282 ,03 medical and health sciences ,Young Adult ,Internal medicine ,Genetics ,Mucositis ,Nasopharyngeal carcinoma ,Humans ,Aged ,Neoplasm Staging ,Proportional Hazards Models ,business.industry ,Induction chemotherapy ,Cancer ,Retrospective cohort study ,medicine.disease ,Radiation therapy ,030104 developmental biology ,Radiotherapy, Intensity-Modulated ,business - Abstract
Background Little is known about the prognostic difference of anti-EGFR therapy, cetuximab (CTX) or nimotuzumab (NTZ), concurrently with induction chemotherapy (IC, investigational arm) or RT (control arm) for patients with locoregionally advanced nasopharyngeal carcinoma (LA-NPC). We conducted this retrospective study to address this. Methods We identified 296 patients with newly diagnosed LA-NPC at Sun Yat-Sen University Cancer Center between January 2012 and May 2015. Patients were treated by IC with CCRT or RT and CTX/NTZ was delivered during IC or radiotherapy. Survival outcomes and toxicities between different arms were compared. Results In total, there were 149 patients in the investigational arm and 147 in control arm. The 3-year disease-free survival, overall survival, distant metastasis-free survival and locoregional relapse-free survival rates for investigational arm vs. control arm were 84.3% vs. 74.3% (P = 0.027), 94.0% vs. 92.1% (P = 0.673), 88.0% vs. 81.8% (P = 0.147) and 93.3% vs. 88.0% (P = 0.093). Multivariate analysis revealed patients in the control arm achieved significantly worse disease-free survival (HR, 1.497; 95% CI, 1.016–2.206; P = 0.026) compared with those in the investigational arm; however, no significant difference was identified for other endpoints. Patients in the investigational arm experienced more grade 3–4 skin reaction (15.4% vs. 2.0%, P
- Published
- 2018
28. Diagnostic value of interleukins for tuberculous pleural effusion: a systematic review and meta-analysis
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Yongchun Shen, Jiangyu Qin, Lei Chen, Fuqiang Wen, Ni Zeng, Chun Wan, Yanqiu Wu, and Ting Yang
- Subjects
Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Sensitivity and Specificity ,03 medical and health sciences ,0302 clinical medicine ,Tuberculous pleural effusion ,Web of knowledge ,Internal medicine ,Diagnosis ,medicine ,Humans ,030212 general & internal medicine ,lcsh:RC705-779 ,Study quality ,Receiver operating characteristic ,Quality assessment ,business.industry ,Interleukins ,Review manager ,lcsh:Diseases of the respiratory system ,Exudates and Transudates ,Tuberculosis, Pleural ,Interleukin ,Pleural Effusion ,Meta-analysis ,030228 respiratory system ,Effusion ,Area Under Curve ,business ,Research Article - Abstract
Background The ability of interleukins (ILs) to differentiate tuberculous pleural effusion from other types of effusion is controversial. The aim of our study was to summarize the evidence for its use of ruling out or in tuberculous pleural effusion. Methods Two investigators independently searched PubMed, EMBASE, Web of Knowledge, CNKI, WANFANG, and WEIPU databases to identify studies assessing diagnostic role of ILs for tuberculous pleural effusion published up to January, 2017. Study quality was assessed using Quality Assessment of Diagnostic Accuracy Studies-2. The pooled diagnostic sensitivity and specificity of ILs were calculated by using Review Manager 5.3. Area under the summary receiver operating characteristic curve (AUC) was used to summarize the overall diagnostic performance of individual markers. Results Thirty-eight studies met our inclusion criteria. Pooled sensitivity, specificity and AUC for chosen ILs were as follows: IL-2, 0.67,0.76 and 0.86; IL-6, 0.86, 0.84 and 0.90; IL-12, 0.78, 0.83 and 0.86; IL-12p40, 0.82,0.65 and 0.76; IL-18, 0.87, 0.92 and 0.95; IL-27, 0.93, 0.95 and 0.95; and IL-33, 0.84, 0.80 and 0.88. Conclusions Some of these ILs may assist in diagnosing tuberculous pleural effusion, though no single IL is likely to show adequate sensitivity or specificity on its own. Further studies on a large scale with better study design should be performed to assess the diagnostic potential of ILs. Electronic supplementary material The online version of this article (10.1186/s12890-017-0530-3) contains supplementary material, which is available to authorized users.
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- 2017
29. Association in a Chinese population of a genetic variation in the early B-cell factor 1 gene with coronary artery disease
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Yao Ma, Jian-Jun Yan, Zhi-Yong Xie, Ya-Fei Li, Lian-Sheng Wang, Lei Chen, and Zhong Chen
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0301 basic medicine ,Male ,medicine.medical_specialty ,China ,Heterozygote ,Coronary Artery Disease ,030204 cardiovascular system & hematology ,Risk Assessment ,Severity of Illness Index ,Coronary artery disease ,03 medical and health sciences ,0302 clinical medicine ,Asian People ,Gene Frequency ,Polymorphism (computer science) ,Risk Factors ,Diabetes mellitus ,Internal medicine ,Genotype ,medicine ,Odds Ratio ,Humans ,Genetic Predisposition to Disease ,Allele ,Allele frequency ,Genetic Association Studies ,Aged ,Genetic polymorphism ,Chi-Square Distribution ,Polymorphism, Genetic ,business.industry ,Homozygote ,Case-control study ,Coronary Stenosis ,Odds ratio ,Middle Aged ,medicine.disease ,030104 developmental biology ,Logistic Models ,Phenotype ,Case-Control Studies ,Multivariate Analysis ,Cardiology ,Trans-Activators ,Female ,business ,Cardiology and Cardiovascular Medicine ,Research Article ,Early B-cell factor 1 - Abstract
Background Early B-cell factor 1 (EBF1) is a transcription factor expressed primarily during early B cell development. Previous studies have shown EBF1 regulates blood glucose and lipid metabolism in mice with diabetes and central adiposity. Recently, a genetic variation (rs36071027) located in an EBF1 gene intron was associated with carotid artery intima-media thickness. However, whether this polymorphism is actually linked with coronary artery disease (CAD) and its severity remains unclear. Methods This study includes 293 CAD cases and 262 controls without CAD. All participants were devided into two groups based on their coronary angiography results. A polymerase chain reaction-ligase detection reaction was used to identify genotypes at rs36071027, and CAD patients were further divided into subgroups with one-, two-, or three-vessel stenosis reflective of CAD severity. Results The frequency of the rs36071027 TT genotype was significantly higher in CAD cases versus controls (4.8% vs. 1.5%, 95% CI: 1.13-10.81 P = 0.029). Subjects with a variant genotype T allele had an increased risk of CAD compared to C allele carriers (additive model: 95% CI: 1.13-2.23, P = 0.008). After adjustment for cardiovascular risk factors, analysis of the additive and dominant models involving rs36071027 also revealed that T allele carriers had a significantly higher risk for CAD than C allele carriers (additive model: OR 1.56, 95% CI 1.10–2.22, P = 0.013; dominant model: OR 1.60, 95% CI 1.07–2.41, P = 0.023). Furthermore, both diabetes and the CT + TT rs36071027 genotype were significantly associated with three-vessel stenosis. Conclusion Our results in a Chinese population suggest that the TT genotype and T alleles in rs36071027 in the EBF1 gene are associated with an increased risk of CAD and its severity. Electronic supplementary material The online version of this article (doi:10.1186/s12872-017-0489-2) contains supplementary material, which is available to authorized users.
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- 2017
30. Casticin induces apoptosis and G0/G1 cell cycle arrest in gallbladder cancer cells
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Huaifeng Li, Xuefeng Wang, Lei Chen, Jianhua Lu, Jun Gu, Xiaoling Song, Wen-Jie Zhang, Fei Zhang, Yun-jiao Zhang, Zheng Wang, Jia-wei Mei, Yuanyuan Ye, Yunping Hu, Yijian Zhang, and Yingbin Liu
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0301 basic medicine ,Cancer Research ,Apoptosis ,Casticin ,Biology ,G0/G1 arrest ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Annexin ,Genetics ,Akt signaling pathway ,Viability assay ,Akt/PKB signaling pathway ,Cell growth ,Cell cycle ,Gallbladder cancer ,Molecular biology ,030104 developmental biology ,Oncology ,chemistry ,030220 oncology & carcinogenesis ,Primary Research ,G1 phase - Abstract
Background Casticin, the flavonoid extracted from Vitex rotundifolia L, exerts various biological effects, including anti-inflammatory and anti-cancer activity. The aim of this study is to investigate the effects and mechanisms of casticin in human gallbladder cancer cells. Methods Human NOZ and SGC996 cells were used to perform the experiments. CCK-8 assay and colony formation assay were performed to evaluate cell viability. Cell cycle analyses and annexin V/PI staining assay for apoptosis were measured using flow cytometry. Western blot analysis was used to evaluate the changes in protein expression, and the effect of casticin treatment in vivo was experimented with xenografted tumors. Results In this study, we found that casticin significantly inhibited gallbladder cancer cell proliferation in a dose- and time-dependent manner. Casticin also induced G0/G1 arrest and mitochondrial-related apoptosis by upregulating Bax, cleaved caspase-3, cleaved caspase-9 and cleaved poly ADP-ribose polymerase expression, and by downregulating Bcl-2 expression. Moreover, casticin induced cycle arrest and apoptosis by upregulating p27 and downregulating cyclinD1/cyclin-dependent kinase4 and phosphorylated protein kinase B. In vivo, casticin inhibited tumor growth. Conclusion Casticin induces G0/G1 arrest and apoptosis in gallbladder cancer, suggesting that casticin might represent a novel and effective agent against gallbladder cancer.
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- 2017
31. T2 relaxation time for intervertebral disc degeneration in patients with upper back pain: initial results on the clinical use of 3.0 Tesla MRI
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linhui Ruan, Wei Ji, Xiaojing Huang, Qinglei Shi, Jiandong Yuan, Lei Chen, Chun Chen, Raoying Xie, Guangjian Jing, and Kai Zhou
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Adult ,Male ,medicine.medical_specialty ,Shoulder ,Cervicothoracic Junction ,Intervertebral Disc Degeneration ,Asymptomatic ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Cervicothoracic junction ,T2 Relaxation Time ,Image Interpretation, Computer-Assisted ,Back pain ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,In patient ,Aged ,medicine.diagnostic_test ,business.industry ,Magnetic resonance imaging ,Intervertebral disc ,Middle Aged ,Magnetic Resonance Imaging ,Sagittal plane ,medicine.anatomical_structure ,ROC Curve ,Radiology Nuclear Medicine and imaging ,Back Pain ,T2 relaxation ,Cervical ,Female ,Radiology ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Neck ,Research Article - Abstract
Background Magnetic resonance imaging (MRI) is a useful non-invasive tool for evaluating abnormalities of intervertebral discs. However, there are few studies which applied functional MRI techniques to investigate degenerative changes in cervical and cervicothoracic junction (CTJ) spine among adults. The aim of this study was to compare T2 relaxation time measurement evaluation with morphological grading for assessing cervical and CTJ intervertebral discs (IVD) in the patients suffering neck, shoulder, and upper back pain. Methods Sixty-three patients (378 IVDs) and 60 asymptomatic volunteers (360 IVDs) of the cervical and CTJ discs were assessed using a 3.0 T magnetic resonance imaging (MRI) protocol, including an sagittal T2 relaxation time protocol. The relaxation time values of the nucleus pulposus (NP) were recorded and all discs were visually graded according to Pfirrman’s grading system. The correlation between T2 relaxation time values and qualitative clinical grading of degeneration, patient age, sex and anatomic level were analyzed Results There is a clear trend of decreasing mean T2 values of the NP associate with increasing Pfirrmann grades (C2-T1) for both patients and asymptotic volunteers. Significant T2 differences were seen among grades I-V (P
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- 2017
32. Knockdown of GGCT inhibits cell proliferation and induces late apoptosis in human gastric cancer
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Ping Dong, Chunhua Hu, Weibin Shi, Wen-Jie Zhang, Wenjie Lv, Hong-Gang Xiang, and Lei Chen
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0301 basic medicine ,Proliferation ,Apoptosis ,Biology ,Biochemistry ,Flow cytometry ,Small hairpin RNA ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,shRNA ,Stomach Neoplasms ,Cell Line, Tumor ,medicine ,Humans ,RNA, Small Interfering ,Molecular Biology ,Cell Proliferation ,Gene knockdown ,medicine.diagnostic_test ,Cell growth ,Lentivirus ,Cancer ,GGCT ,medicine.disease ,Cell biology ,G2 Phase Cell Cycle Checkpoints ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,M Phase Cell Cycle Checkpoints ,RNA Interference ,Gastric cancer ,gamma-Glutamylcyclotransferase ,Research Article - Abstract
Background Gamma glutamylcyclotransferase (GGCT) has been proved to be involved in various cancers, but the biological function of GGCT in gastric cancer is still largely unknown. Methods The expression level of GGCT was evaluated by informatics analyses based on the Oncomine database. GGCT gene was then effectively knocked down via lentivirus mediated short hairpin RNA (shRNA) system. Then a series of functional assays, including MTT, colony formation and flow cytometry analysis were conducted on gastric cancer cells following GGCT knockdown. Results We found GGCT is commonly up-regulated in gastric cancer tissues. Furthermore, MTT analysis showed that GGCT depletion significantly inhibited cell proliferation in MGC80-3 and AGS cells. Colony formation assay revealed that depletion of GGCT reduced the colony formation ability in gastric cancer cells. What’s more, cell cycle analysis showed that depletion of GGCT induced gastric cancer cell cycle arrested G2/M phase. More importantly, cell apoptosis analysis further revealed that GGCT inhibition induced early and late cell apoptosis in gastric cancer. Conclusion This study suggests GGCT is essential for gastric cancer proliferation and its downregulation may provide a potential anticancer therapy for gastric cancer.
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- 2016
33. Uncovering the active compounds and effective mechanisms of the dried mature sarcocarp of Cornus officinalis Sieb. Et Zucc. For the treatment of Alzheimer's disease through a network pharmacology approach.
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Yan-Jie Qu, Rong-Rong Zhen, Li-Min Zhang, Chao Gu, Lei Chen, Xiao Peng, Bing Hu, and Hong-Mei An
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AUTOPHAGY ,ALZHEIMER'S disease ,APOPTOSIS ,CELLULAR signal transduction ,HERBAL medicine ,CHINESE medicine ,ORGANIC compounds ,PHARMACOLOGY ,RESEARCH funding ,TRANSFERASES ,TUMOR necrosis factors ,OXIDATIVE stress ,THERAPEUTICS - Abstract
Background: Shanzhuyu (the dried mature sarcocarp of Cornus officinalis Sieb. et Zucc., DMSCO) is a Chinese herb that can be used for the treatment of Alzheimer's disease (AD), but its mechanism remains unknown. The present study aimed to investigate the active ingredients and effective mechanisms of DMSCO for the treatment of AD based on a network pharmacology approach. Methods: The active components of DMSCO were collected from the TCMSP and ETCM databases and the target proteins of these compounds were predicted using TCMSP, SwissTargetPrediction and the STITCH database. The AD-related target proteins were identified from the OMIM, DisGeNet, GEO and GeneCards databases. The network interaction model of the compound-target-disease was established and was used to obtain the key targets of DMSCO on AD through network topology analysis. Subsequently, gene enrichment in Gene Ontology (GO) and KEGG pathways were conducted using the David 6.8 online tool. Results: A total of 30 DMSCO effective compounds and 209 effective drug targets were obtained. A total of 172 AD-related genes and 37 shared targets of DMSCO and AD were identified. A total of 43 key targets for the treatment of AD were obtained from the topological analysis of the DMSCO-AD target network. These key targets were involved in a variety of biological processes, including amyloid deposition, apoptosis, autophagy, inflammatory response and oxidative stress and pathways, such as the PI3K-AKT, MAPK and TNF pathways. Three key compounds, namely ursolic acid, anethole and ß-sitosterol were obtained from the analysis of the key targets. Conclusions: Ursolic acid, anethole and ß-sitosterol may be the main active components of DMSCO in the treatment of AD. DMSCO can treat AD by regulating amyloid deposition, apoptosis, autophagy, inflammatory response and oxidative stress via the PI3K-AKT, MAPK and other signaling pathways. [ABSTRACT FROM AUTHOR]
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- 2020
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34. Liver tumor segmentation in CT volumes using an adversarial densely connected network.
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Lei Chen, Hong Song, Chi Wang, Yutao Cui, Jian Yang, Xiaohua Hu, and Le Zhang
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LIVER cancer , *CONE beam computed tomography , *HEPATIC veins , *ABDOMINAL tumors , *CANCER , *ARTIFICIAL neural networks - Abstract
Background: Malignant liver tumor is one of the main causes of human death. In order to help physician better diagnose and make personalized treatment schemes, in clinical practice, it is often necessary to segment and visualize the liver tumor from abdominal computed tomography images. Due to the large number of slices in computed tomography sequence, developing an automatic and reliable segmentation method is very favored by physicians. However, because of the noise existed in the scan sequence and the similar pixel intensity of liver tumors with their surrounding tissues, besides, the size, position and shape of tumors also vary from one patient to another, automatic liver tumor segmentation is still a difficult task. Results: We perform the proposed algorithm to the Liver Tumor Segmentation Challenge dataset and evaluate the segmentation results. Experimental results reveal that the proposed method achieved an average Dice score of 68.4% for tumor segmentation by using the designed network, and ASD, MSD, VOE and RVD improved from 27.8 to 21,147 to 124,0.52 to 0.46 and 0.69 to 0.73, respectively after performing adversarial training strategy, which proved the effectiveness of the proposed method. Conclusions: The testing results show that the proposed method achieves improved performance, which corroborated the adversarial training based strategy can achieve more accurate and robustness results on liver tumor segmentation task. [ABSTRACT FROM AUTHOR]
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- 2019
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35. Genome-wide analysis of long non-coding RNAs at early stage of skin pigmentation in goats (Capra hircus)
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Li Nianfu, Lei Chen, Sun Xiaoyan, Liu Liangjia, Ren Hangxing, Jiang Jing, Gaofu Wang, Jinhong Zhao, and Zhou Peng
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0301 basic medicine ,Skin Pigmentation ,Biology ,Proteomics ,Genome ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Intergenic region ,lncRNA ,Fetus ,Pregnancy ,Genetics ,Capra hircus ,Animals ,Gene ,Skin ,integumentary system ,Pigmentation ,Goats ,Introns ,White (mutation) ,030104 developmental biology ,030220 oncology & carcinogenesis ,Goat ,Female ,RNA, Long Noncoding ,DNA microarray ,Biotechnology ,Research Article - Abstract
Background Long noncoding RNAs (lncRNAs) play roles in almost all biological processes; however, their function and profile in skin development and pigmentation is less understood. In addition, because lncRNAs are species-specific, their function in goats has not been established. Result We systematically identified lncRNAs in 100-day-old fetal skin by deep RNA-sequencing using the Youzhou dark goat (dark skin) and Yudong white goat (white skin) as a model of skin pigmentation. A total of 841,895,634 clean reads were obtained from six libraries (samples). We identified 1336 specific lncRNAs in fetal skin that belonged to three subtypes, including 999 intergenic lncRNAs (lincRNAs), 218 anti-sense lncRNAs, and 119 intronic lncRNAs. Our results demonstrated significant differences in gene architecture and expression among the three lncRNA subtypes, particularly in terms of density and position bias of transpose elements near the transcription start site. We also investigated the impact of lncRNAs on its target genes in cis and trans, indicating that these lncRNAs have a strict tissue specificity and functional conservation during skin development and pigmentation. Conclusion The present study provides a resource for lncRNA studies in diseases involved in pigmentation and skin development. It expands our knowledge about lncRNA biology as well as contributes to the annotation of the goat genome. Electronic supplementary material The online version of this article (doi:10.1186/s12864-016-2365-3) contains supplementary material, which is available to authorized users.
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- 2016
36. Antioxidative phytochemicals from Rhododendron oldhamii Maxim. leaf extracts reduce serum uric acid levels in potassium oxonate-induced hyperuricemic mice
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Shang Tse Ho, Chien Chao Chiu, Yu Tang Tung, Ya Ling Liu, Hsiao Li Chuang, Jyh-Horng Wu, Chi Chang Huang, Chi Yang Lin, and Lei Chen Lin
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Male ,Chronic bronchitis ,Online HPLC–DPPH ,Antioxidant ,Rhododendron ,medicine.medical_treatment ,Phytochemicals ,Hyperuricemia ,Phytochemical ,Kidney ,Antioxidants ,law.invention ,Gout Suppressants ,chemistry.chemical_compound ,Benzbromarone ,Mice ,law ,Medicine ,Animals ,Mice, Inbred ICR ,Traditional medicine ,business.industry ,Plant Extracts ,Rhododendron oldhamii maxim ,General Medicine ,medicine.disease ,Quercitrin ,Uric Acid ,Plant Leaves ,Leaf ,Disease Models, Animal ,Oxonic Acid ,Complementary and alternative medicine ,chemistry ,Uric acid ,business ,Phytotherapy ,Research Article - Abstract
Background Some of the genus Rhododendron was used in traditional medicine for arthritis, acute and chronic bronchitis, asthma, pain, inflammation, rheumatism, hypertension and metabolic diseases and many species of the genus Rhododendron contain a large number of phenolic compounds and antioxidant properties that could be developed into pharmaceutical products. Methods In this study, the antioxidative phytochemicals of Rhododendron oldhamii Maxim. leaves were detected by an online HPLC–DPPH method. In addition, the anti-hyperuricemic effect of the active phytochemicals from R. oldhamii leaf extracts was investigated using potassium oxonate (PO)-induced acute hyperuricemia. Results Six phytochemicals, including (2R, 3R)-epicatechin (1), (2R, 3R)-taxifolin (2), (2R, 3R)-astilbin (3), hyposide (4), guaijaverin (5), and quercitrin (6), were isolated using the developed screening method. Of these, compounds 3, 4, 5, and 6 were found to be major bioactive phytochemicals, and their contents were determined to be 130.8 ± 10.9, 105.5 ± 8.5, 104.1 ± 4.7, and 108.6 ± 4.0 mg per gram of EtOAc fraction, respectively. In addition, the four major bioactive phytochemicals at the same dosage (100 mmol/kg) were administered to the abdominal cavity of potassium oxonate (PO)-induced hyperuricemic mice, and the serum uric acid level was measured after 3 h of administration. H&E staining showed that PO-induced kidney injury caused renal tubular epithelium nuclear condensation in the cortex areas or the appearance of numerous hyaline casts in the medulla areas; treatment with 100 mmol/kg of EtOAc fraction, (2R, 3R)-astilbin, hyposide, guaijaverin, and quercitrin significantly reduced kidney injury. In addition, the serum uric acid level was significantly suppressed by 54.1, 35.1, 56.3, 56.3, and 53.2 %, respectively, by the administrations of 100 mmol/kg EtOAc fraction and the derived major phytochemicals, (2R, 3R)-astilbin, hyposide, guaijaverin, and quercitrin, compared to the PO group. The administration of 10 mg/kg benzbromarone, a well-known uricosuric agent, significantly reduced the serum uric acid level by 45.5 % compared to the PO group. Conclusion The in vivo decrease in uric acid was consistent with free radical scavenging activity, indicating that the major phytochemicals of R. oldhamii leave extracts and the derived phytochemicals possess potent hypouricemic effects, and they could be potential candidates for new hypouricemic agents.
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- 2015
37. Identification of surrogate endpoints in patients with locoregionally advanced nasopharyngeal carcinoma receiving neoadjuvant chemotherapy plus concurrent chemoradiotherapy versus concurrent chemoradiotherapy alone
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Yu Pei Chen, Lei Chen, Mu Sheng Zeng, Jun Ma, Tie Bang Kang, Jian Yong Shao, Wei Hua Jia, Wen Na Zhang, Guan Qun Zhou, Yan Ping Mao, Ling Long Tang, Hai Qiang Mai, Ying Sun, and Xu Liu
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Oncology ,Adult ,Male ,Cancer Research ,medicine.medical_specialty ,medicine.medical_treatment ,Nasopharyngeal neoplasm ,Neoadjuvant chemotherapy ,Young Adult ,Internal medicine ,Genetics ,medicine ,Carcinoma ,Humans ,Overall survival ,Neoadjuvant therapy ,Survival analysis ,health care economics and organizations ,Aged ,Retrospective Studies ,Nasopharyngeal Carcinoma ,business.industry ,Surrogate endpoint ,Nasopharyngeal Neoplasms ,Chemoradiotherapy ,Middle Aged ,medicine.disease ,Survival Analysis ,Neoadjuvant Therapy ,Concurrent chemoradiotherapy ,Radiation therapy ,Nasopharyngeal carcinoma ,Female ,business ,Research Article - Abstract
Background In the era of intensity-modulated radiotherapy (IMRT), the efficacy of additional neoadjuvant chemotherapy (NACT) to concurrent chemoradiotherapy (CCRT) in locoregionally advanced nasopharyngeal carcinoma (NPC) is currently being investigated in ongoing trials. Overall survival (OS) is the gold standard endpoint in NPC trials. We performed this analysis to identify surrogate endpoints for OS, which could shorten follow-up duration and speed up assessment of treatment effects. Methods We retrospectively analysed 208 matched-pair patients with locoregionally advanced NPC receiving NACT+CCRT or CCRT. Progression-free survival (PFS), failure-free survival (FFS), distant failure-free survival (D-FFS) and locoregional failure-free survival (LR-FFS) at 2 and 3 years were assessed as surrogates for 5-year OS according to Prentice’s criteria. The strength of the associations were assessed using Spearman’s rank correlation coefficient. Results No significant differences were observed between treatment arms for any surrogate endpoint at 2 years, which rejected Prentice’s second criterion. In contrast, 3-year LR-FFS, PFS, FFS and D-FFS were consistent with all four of Prentice’s criteria; the rank correlation coefficient (0.730) between 3-year PFS and 5-year OS was highest. Conclusions 3-year PFS, FFS and D-FFS could be valid surrogate endpoints for 5-year OS; 3-year PFS may be the most accurate.
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- 2015
38. MiR-362-5p promotes the malignancy of chronic myelocytic leukaemia via down-regulation of GADD45α
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Peng, Yang, Fang, Ni, Rui-Qing, Deng, Guo, Qiang, Hua, Zhao, Ming-Zhen, Yang, Xin-Yi, Wang, You-Zhi, Xu, Li, Chen, Dan-Lei, Chen, Zhi-Jun, Chen, Li-Xin, Kan, and Si-Ying, Wang
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Cancer Research ,Down-Regulation ,Mice, Nude ,Apoptosis ,Cell Cycle Proteins ,Real-Time Polymerase Chain Reaction ,Mice ,Cell Movement ,Cell Line, Tumor ,Leukemia, Myelogenous, Chronic, BCR-ABL Positive ,Animals ,Humans ,CML ,Oncogene ,Cell Proliferation ,Mice, Inbred BALB C ,Research ,Cell Cycle ,Nuclear Proteins ,P38 ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,HEK293 Cells ,Oncology ,Molecular Medicine ,GADD45α ,Female ,miR-362-5p ,K562 Cells ,JNK1/2 - Abstract
Background MicroRNAs (miR, miRNAs) play pivotal roles in numerous physiological and pathophysiological contexts. We investigated whether miR-362-5p act as an oncogene in chronic myeloid leukaemia (CML) and aimed to understand its potential underlying mechanisms. Methods We compared the miR-362-5p expression levels between CML and non-CML cell lines, and between fresh blood samples from CML patients and normal healthy controls using quantitative real-time PCR (qPCR). Cell counting kit-8 (CCK-8) and Annexin V-FITC/PI analyses were used to measure the effects of miR-362-5p on proliferation and apoptosis, and Transwell assays were used to evaluate migration and invasion. A xenograft model was used to examine in vivo tumourigenicity. The potential target of miR-362-5p was confirmed by a luciferase reporter assay, qPCR and western blotting. Involvement of the JNK1/2 and P38 pathways was investigated by western blotting. Results miR-362-5p was up-regulated in CML cell lines and fresh blood samples from CML patients, and was associated with Growth arrest and DNA damage-inducible (GADD)45α down-regulation. Inhibition of miR-362-5p simultaneously repressed tumour growth and up-regulated GADD45α expression in a xenograft model. Consistently, the knockdown of GADD45α expression partially neutralized the effects of miR-362-5p inhibition. Furthermore study suggested that GADD45α mediated downstream the effects of miR-362-5p, which might indirectly regulates the activation of the JNK1/2 and P38 signalling pathways. Conclusion miR-362-5p acts as an oncomiR that down-regulates GADD45α, which consequently activates the JNK1/2 and P38 signalling. This finding provides novel insights into CML leukaemogenesis and may help identify new diagnostic and therapeutic targets. Electronic supplementary material The online version of this article (doi:10.1186/s12943-015-0465-3) contains supplementary material, which is available to authorized users.
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- 2015
39. Resistance integrons: class 1, 2 and 3 integrons
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Yang Deng, Yanmei Li, Dingqiang Chen, Xuerui Bao, Junyan Liu, Jian Miao, Lei Chen, Lili Ji, Huawei Bian, and Guangchao Yu
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Microbiology (medical) ,medicine.medical_specialty ,China ,medicine.drug_class ,Antibiotics ,Horizontal transfer ,Drug resistance ,Review ,Biology ,Antimicrobial resistance ,Communicable Diseases ,Integrons ,Antibiotic resistance ,Medical microbiology ,Environmental health ,Drug Resistance, Bacterial ,medicine ,Animals ,Humans ,Medical prescription ,Resistance (ecology) ,business.industry ,Public health ,Agriculture ,General Medicine ,Drug Utilization ,Biotechnology ,Anti-Bacterial Agents ,Resistance integrons ,Infectious Diseases ,Mobile genetic elements ,business - Abstract
As recently indiscriminate abuse of existing antibiotics in both clinical and veterinary treatment leads to proliferation of antibiotic resistance in microbes and poses a dilemma for the future treatment of such bacterial infection, antimicrobial resistance has been considered to be one of the currently leading concerns in global public health, and reported to widely spread and extended to a large variety of microorganisms. In China, as one of the currently worst areas for antibiotics abuse, the annual prescription of antibiotics, including both clinical and veterinary treatment, has approaching 140 gram per person and been roughly estimated to be 10 times higher than that in the United Kingdom, which is considered to be a potential area for the emergence of “Super Bugs”. Based on the integrons surveillance in Guangzhou, China in the past decade, this review thus aimed at summarizing the role of integrons in the perspective of both clinical setting and environment, with the focus on the occurrence and prevalence of class 1, 2 and 3 integrons.
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- 2015
40. Intraoperative high-field magnetic resonance imaging combined with functional neuronavigation in resection of low-grade temporal lobe tumors
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Jiefeng Geng, Xiao Lei Chen, Shao-cong Bai, Bai-Nan Xu, Xin-Guang Yu, Dong-Dong Wu, and Shi-Hui Wei
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Adult ,Male ,medicine.medical_specialty ,Neuronavigation ,Intraoperative magnetic resonance imaging ,Return to work ,Adolescent ,Interventional magnetic resonance imaging ,Low-grade glioma ,Seizure outcome ,Temporal lobe ,Lesional temporal lobe epilepsy ,Epilepsy ,Young Adult ,Postoperative Complications ,Surgical oncology ,Glioma ,Monitoring, Intraoperative ,medicine ,Humans ,Prospective Studies ,Prospective cohort study ,Child ,Aged ,medicine.diagnostic_test ,business.industry ,Brain Neoplasms ,Research ,Magnetic resonance imaging ,Middle Aged ,medicine.disease ,Prognosis ,Magnetic Resonance Imaging ,Temporal Lobe ,Surgery ,Oncology ,Case-Control Studies ,Quality of Life ,Female ,Radiology ,Neoplasm Grading ,business ,Follow-Up Studies - Abstract
Background The aim of this study is to investigate the role of intraoperative MR imaging in temporal lobe low-grade glioma (LGG) surgery and to report the surgical outcome in our series with regard to seizures, neurological defects, and quality of life. Methods Patients with temporal lobe contrast-nonenhancing gliomas who presented with seizures in the course of their disease were enrolled in our prospective study. We non-randomly assigned patients to undergo intraoperative magnetic resonance imaging (iMRI)-guided surgery or conventional surgery. Extent of resection (EOR) and surgical outcomes were compared between the two groups. Results Forty-one patients were allocated in the iMRI group, and 14 were in the conventional group. Comparable EOR was achieved for the two groups (p = 0.634) although preoperative tumor volumes were significantly larger for the iMRI group. Seizure outcome tended to be better for the iMRI group (Engel class I achieved for 89.7 % (35/39) vs 75 % (9/12)) although this difference was not statistically different. Newly developed neurological deficits were observed in four patients (10.3 %) and two patients (16.7 %), respectively (p = 0.928). Free of seizures and neurological morbidity led to a return-to-work or return-to-school rate of 84.6 % (33/39) vs 75 % (9/12), respectively (p = 0.741). Conclusions Our study provided evidence that iMRI was a safe and useful tool in temporal lobe LGG surgery. Optimal extent of resection contributed to favorable seizure outcome in our series with low morbidity rate, which led to a high return-to-work rate.
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- 2015
41. Metabolomic basis of laboratory evolution of butanol tolerance in photosynthetic Synechocystis sp. PCC 6803
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Jiangxin Wang, Mengliang Shi, Yaxing Wang, Weiwen Zhang, Xiangfeng Niu, Lianju Gao, Lei Chen, and Xiaoqing Zhang
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Cyanobacteria ,Evolution ,Butanols ,Bioengineering ,Biology ,Photosynthesis ,Applied Microbiology and Biotechnology ,Phosphoenolpyruvic acid ,Microbiology ,chemistry.chemical_compound ,Metabolomics ,Glycerol ,Butanol ,Research ,Synechocystis ,biology.organism_classification ,chemistry ,Biochemistry ,bacteria ,Stearic acid ,Directed Molecular Evolution ,Tolerance ,Biotechnology - Abstract
Background Recent efforts demonstrated the potential application of cyanobacteria as a “microbial cell factory” to produce butanol directly from CO2. However, cyanobacteria have very low tolerance to the toxic butanol, which limits the economic viability of this renewable system. Results Through a long-term experimental evolution process, we achieved a 150% increase of the butanol tolerance in a model cyanobacterium Synechocystis sp. PCC 6803 after a continuous 94 passages for 395 days in BG11 media amended with gradually increased butanol concentration from 0.2% to 0.5% (v/v). To decipher the molecular mechanism responsible for the tolerance increase, we employed an integrated GC-MS and LC-MS approach to determine metabolomic profiles of the butanol-tolerant Synechocystis strains isolated from several stages of the evolution, and then applied PCA and WGCNA network analyses to identify the key metabolites and metabolic modules related to the increased tolerance. The results showed that unstable metabolites of 3-phosphoglyceric acid (3PG), D-fructose 6-phosphate (F6P), D-glucose 6-phosphate (G6P), NADPH, phosphoenolpyruvic acid (PEP), D-ribose 5-phosphate (R5P), and stable metabolites of glycerol, L-serine and stearic acid were differentially regulated during the evolution process, which could be related to tolerance increase to butanol in Synechocystis. Conclusions The study provided the first time-series description of the metabolomic changes related to the gradual increase of butanol tolerance, and revealed a metabolomic basis important for rational tolerance engineering in Synechocystis. Electronic supplementary material The online version of this article (doi:10.1186/s12934-014-0151-y) contains supplementary material, which is available to authorized users.
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- 2014
42. CFD and experimental studies on capture of fine particles by liquid droplets in open spray towers.
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Rafidi, Nabil, Brogaard, Fredrik, Lei Chen, Håkansson, Rikard, and Tabikh, Ali
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- 2018
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43. Ginsenoside G-Rh2 synergizes with SMI-4a in anti-melanoma activity through autophagic cell death.
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Da-lun Lv, Lei Chen, Wei Ding, Wei Zhang, He-li Wang, Shuai Wang, and Wen-bei Liu
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AUTOPHAGY , *ANIMAL experimentation , *ANTINEOPLASTIC agents , *APOPTOSIS , *CELL death , *CELLULAR signal transduction , *DRUG synergism , *FLOW cytometry , *GLYCOSIDES , *MELANOMA , *MICE , *WESTERN immunoblotting , *PROTEIN kinase inhibitors , *IN vivo studies , *PHARMACODYNAMICS - Abstract
Background: Melanoma is a leading cause of cancer death worldwide, and SMI-4a and G-Rh2 exert anti-tumor activity in multiple cancer. However, SMI-4a as well as a synergistic relationship between SMI-4a and G-Rh2 in anti-melanoma capacity are still unknown. Therefore, we investigated the effects of SMI-4a and combined SMI-4a with G-Rh2 on the viability, apoptosis and autophagy of melanoma, and to preliminarily explore the underlying mechanism of SMI-4a and combined SMI-4a with G-Rh2 in inhibiting tumor growth. Methods: Cell viability was examined with cell counting Kit 8 assay and colony formation assay; Apoptosis was evaluated by flow cytometry and Caspase 3/7 activity assay; Western blotting was used to test proteins related to autophagy and the AKT/mammalian target of rapamycin (mTOR) signaling pathway; Tumor xenograft model in BALB/c nude mice was performed to evaluate the effects of SMI-4a and combined SMI-4a with G-Rh2 in anti-melanoma in vivo. Results: SMI-4a, a pharmacological inhibitor of PIM-1, could decrease cell viability, induce apoptosis, and promote Caspase 3/7 activity in both A375 and G361 melanoma cells, and SMI-4a inhibited tumor growth by inducing autophagy via down-regulating AKT/mTOR axis in melanoma cells. Furthermore, G-Rh2 amplified the anti-tumor activity of SMI-4a in melanoma cells via strengthening autophagy. Conclusions: Our results suggested that SMI-4a could enhance autophagy-inducing apoptosis by inhibiting AKT/ mTOR signaling pathway in melanoma cells, and G-Rh2 could enhance the effects of SMI-4a against melanoma cancer via amplifying autophagy induction. This study demonstrates that combined SMI-4a and G-Rh2 might be a novel alternative strategy for melanoma treatment. [ABSTRACT FROM AUTHOR]
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- 2018
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44. Automatic schizophrenic discrimination on fNIRS by using complex brain network analysis and SVM.
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Hong Song, Lei Chen, RuiQi Gao, Mihaita Bogdan, Iordachescu Ilie, Jian Yang, Shuliang Wang, Wentian Dong, Wenxiang Quan, Weimin Dang, Xin Yu, Song, Hong, Chen, Lei, Gao, RuiQi, Bogdan, Iordachescu Ilie Mihaita, Yang, Jian, Wang, Shuliang, Dong, Wentian, Quan, Wenxiang, Dang, Weimin, and Yu, Xin
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SCHIZOPHRENIA , *MENTAL illness , *NEAR infrared spectroscopy , *HEMOGLOBINS , *BRAIN - Abstract
Background: Schizophrenia is a kind of serious mental illness. Due to the lack of an objective physiological data supporting and a unified data analysis method, doctors can only rely on the subjective experience of the data to distinguish normal people and patients, which easily lead to misdiagnosis. In recent years, functional Near-Infrared Spectroscopy (fNIRS) has been widely used in clinical diagnosis, it can get the hemoglobin concentration through the variation of optical intensity.Methods: Firstly, the prefrontal brain networks were constructed based on oxy-Hb signals from 52-channel fNIRS data of schizophrenia and healthy controls. Then, Complex Brain Network Analysis (CBNA) was used to extract features from the prefrontal brain networks. Finally, a classier based on Support Vector Machine (SVM) is designed and trained to discriminate schizophrenia from healthy controls. We recruited a sample which contains 34 healthy controls and 42 schizophrenia patients to do the one-back memory task. The hemoglobin response was measured in the prefrontal cortex during the task using a 52-channel fNIRS system.Results: The experimental results indicate that the proposed method can achieve a satisfactory classification with the accuracy of 85.5%, 92.8% for schizophrenia samples and 76.5% for healthy controls. Also, our results suggested that fNIRS has the potential capacity to be an effective objective biomarker for the diagnosis of schizophrenia.Conclusions: Our results suggested that, using the appropriate classification method, fNIRS has the potential capacity to be an effective objective biomarker for the diagnosis of schizophrenia. [ABSTRACT FROM AUTHOR]- Published
- 2017
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45. Isoprenaline/β2-AR activates Plexin-A1/VEGFR2 signals via VEGF secretion in gastric cancer cells to promote tumor angiogenesis.
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Yanjie Lu, Qian Xu, Yanzhen Zuo, Lei Liu, Shaochen Liu, Lei Chen, Kang Wang, Yuntao Lei, Xiangyang Zhao, Yuhong Li, Lu, Yanjie, Xu, Qian, Zuo, Yanzhen, Liu, Lei, Liu, Shaochen, Chen, Lei, Wang, Kang, Lei, Yuntao, Zhao, Xiangyang, and Li, Yuhong
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VASCULAR endothelial growth factor receptors ,NEOVASCULARIZATION ,CANCER cells ,CELL migration ,CYTOLOGY - Abstract
Background: The role of stress signals in regulating gastric cancer initiation and progression is not quite clear. It is known that stress signals modulate multiple processes such as immune function, cell migration and angiogenesis. However, few studies have investigated the mechanisms of how stress signals contribute to gastric cancer angiogenesis.Methods: Here, we used β2-adrenergic receptor (β2-AR) agonist isoprenaline to imitate a stress signal and demonstrated the molecular mechanism underlying stress's influence on tumor angiogenesis.Results: We found that isoprenaline stimulated vascular endothelial growth factor (VEGF) secretion in gastric cancer cells and plexin-A1 expression was induced by human recombinant VEGF165 in both gastric cancer cells and vascular endothelial cells. Furthermore, interfere with plexin-A1 expression in gastric cancer cells influence HUVEC tube formation, migration and tumor growth in vivo.Conclusions: These findings suggest that isoprenaline stimulate VGEF secretion and subsequently up-regulate the expression of plexin-A1 and VEGFR2 in gastric cancer cells, which form a positive impetus to promote tumor angiogenesis. This study reveals a novel molecular mechanism that a stress signal like isoprenaline may enhance angiogenesis via activating plexin-A1/VEGFR2 signaling pathway in gastric cancer, which may be a potential target in development of an anti-angiogenic therapy for gastric cancer. [ABSTRACT FROM AUTHOR]- Published
- 2017
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46. Impaired regeneration in calpain-3 null muscle is associated with perturbations in mTORC1 signaling and defective mitochondrial biogenesis.
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Yalvac, Mehmet E., Amornvit, Jakkrit, Braganza, Cilwyn, Lei Chen, Hussain, Syed-Rehan A., Shontz, Kimberly M., Montgomery, Chrystal L., Flanigan, Kevin M., Lewis, Sarah, and Sahenk, Zarife
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EXTRACHROMOSOMAL DNA ,MITOCHONDRIA formation ,SKELETAL muscle ,MITOCHONDRIA ,MITOCHONDRIAL pathology ,ANATOMY - Abstract
Background: Previous studies in patients with limb-girdle muscular dystrophy type 2A (LGMD2A) have suggested that calpain-3 (CAPN3) mutations result in aberrant regeneration in muscle. Methods: To gain insight into pathogenesis of aberrant muscle regeneration in LGMD2A, we used a paradigm of cardiotoxin (CTX)-induced cycles of muscle necrosis and regeneration in the CAPN3-KO mice to simulate the early features of the dystrophic process in LGMD2A. The temporal evolution of the regeneration process was followed by assessing the oxidative state, size, and the number of metabolic fiber types at 4 and 12 weeks after last CTX injection. Muscles isolated at these time points were further investigated for the key regulators of the pathways involved in various cellular processes such as protein synthesis, cellular energy status, metabolism, and cell stress to include Akt/mTORC1 signaling, mitochondrial biogenesis, and AMPK signaling. TGF-β and microRNA (miR-1, miR-206, miR-133a) regulation were also assessed. Additional studies included in vitro assays for quantifying fusion index of myoblasts from CAPN3-KO mice and development of an in vivo gene therapy paradigm for restoration of impaired regeneration using the adeno-associated virus vector carrying CAPN3 gene in the muscle. Results: At 4 and 12 weeks after last CTX injection, we found impaired regeneration in CAPN3-KO muscle characterized by excessive numbers of small lobulated fibers belonging to oxidative metabolic type (slow twitch) and increased connective tissue. TGF-β transcription levels in the regenerating CAPN3-KO muscles were significantly increased along with microRNA dysregulation compared to wild type (WT), and the attenuated radial growth of muscle fibers was accompanied by perturbed Akt/mTORC1 signaling, uncoupled from protein synthesis, through activation of AMPK pathway, thought to be triggered by energy shortage in the CAPN3-KO muscle. This was associated with failure to increase mitochondria content, PGC-1α, and ATP5D transcripts in the regenerating CAPN3-KO muscles compared to WT. In vitro studies showed defective myotube fusion in CAPN3-KO myoblast cultures. Replacement of CAPN3 by gene therapy in vivo increased the fiber size and decreased the number of small oxidative fibers. Conclusion: Our findings provide insights into understanding of the impaired radial growth phase of regeneration in calpainopathy. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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47. Diagnostic value of interleukins for tuberculous pleural effusion: a systematic review and meta-analysis.
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Ni Zeng, Chun Wan, Jiangyu Qin, Yanqiu Wu, Ting Yang, Yongchun Shen, Fuqiang Wen, Lei Chen, Zeng, Ni, Wan, Chun, Qin, Jiangyu, Wu, Yanqiu, Yang, Ting, Shen, Yongchun, Wen, Fuqiang, and Chen, Lei
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INTERLEUKINS ,TUBERCULOSIS ,PLEURAL effusions ,SENSITIVITY analysis - Abstract
Background: The ability of interleukins (ILs) to differentiate tuberculous pleural effusion from other types of effusion is controversial. The aim of our study was to summarize the evidence for its use of ruling out or in tuberculous pleural effusion.Methods: Two investigators independently searched PubMed, EMBASE, Web of Knowledge, CNKI, WANFANG, and WEIPU databases to identify studies assessing diagnostic role of ILs for tuberculous pleural effusion published up to January, 2017. Study quality was assessed using Quality Assessment of Diagnostic Accuracy Studies-2. The pooled diagnostic sensitivity and specificity of ILs were calculated by using Review Manager 5.3. Area under the summary receiver operating characteristic curve (AUC) was used to summarize the overall diagnostic performance of individual markers.Results: Thirty-eight studies met our inclusion criteria. Pooled sensitivity, specificity and AUC for chosen ILs were as follows: IL-2, 0.67,0.76 and 0.86; IL-6, 0.86, 0.84 and 0.90; IL-12, 0.78, 0.83 and 0.86; IL-12p40, 0.82,0.65 and 0.76; IL-18, 0.87, 0.92 and 0.95; IL-27, 0.93, 0.95 and 0.95; and IL-33, 0.84, 0.80 and 0.88.Conclusions: Some of these ILs may assist in diagnosing tuberculous pleural effusion, though no single IL is likely to show adequate sensitivity or specificity on its own. Further studies on a large scale with better study design should be performed to assess the diagnostic potential of ILs. [ABSTRACT FROM AUTHOR]- Published
- 2017
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48. The R.I.R.S. scoring system: An innovative scoring system for predicting stone-free rate following retrograde intrarenal surgery.
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Yinglong Xiao, Deng Li, Lei Chen, Yaoting Xu, Dingguo Zhang, Yi Shao, Jun Lu, Xiao, Yinglong, Li, Deng, Chen, Lei, Xu, Yaoting, Zhang, Dingguo, Shao, Yi, and Lu, Jun
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KIDNEY surgery ,ANGIOTENSINS ,ATRIAL fibrillation ,NEPHRECTOMY ,MORTALITY - Abstract
Background: To establish and internally validate an innovative R.I.R.S. scoring system that allows urologists to preoperatively estimate the stone-free rate (SFR) after retrograde intrarenal surgery (RIRS).Methods: This study included 382 eligible samples from a total 573 patients who underwent RIRS from January 2014 to December 2016. Four reproducible factors in the R.I.R.S. scoring system, including renal stone density, inferior pole stone, renal infundibular length and stone burden, were measured based on preoperative computed tomography of urography to evaluate the possibility of stone clearance after RIRS.Results: The median cumulative diameter of the stones was 14 mm, and the interquartile range was 10 to 21. The SFR on postoperative day 1 in the present cohort was 61.5% (235 of 382), and the final SFR after 1 month was 73.6% (281 of 382). We established an innovative scoring system to evaluate SFR after RIRS using four preoperative characteristics. The range of the R.I.R.S. scoring system was 4 to 10. The overall score showed a great significance of stone-free status (p < 0.001). The area under the receiver operating characteristic curve of the R.I.R.S. scoring system was 0.904.Conclusions: The R.I.R.S. scoring system is associated with SFR after RIRS. This innovative scoring system can preoperatively assess treatment success after intrarenal surgery and can be used for preoperative surgical arrangement and comparisons of outcomes among different centers and within a center over time. [ABSTRACT FROM AUTHOR]- Published
- 2017
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49. "What do you know?"--knowledge among village doctors of lead poisoning in children in rural China.
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Ruixue Huang, Huacheng Ning, Baum, Carl R., Lei Chen, Hsiao, Allen, Huang, Ruixue, Ning, Huacheng, and Chen, Lei
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LEAD poisoning ,CHILDREN'S health ,RURAL health ,CROSS-sectional method ,QUESTIONNAIRES ,HEALTH attitudes ,PHYSICIANS ,PSYCHOLOGY of physicians ,RESEARCH funding ,RURAL health services - Abstract
Background: This study evaluates the extent of village doctors' knowledge of lead poisoning in children in rural China and assesses the characteristics associated with possessing accurate knowledge.Methods: A cross-sectional, questionnaire-based survey of 297 village doctors in Fenghuang County, Hunan Province, China was conducted. All village doctors were interviewed face-to-face using a "What do you know" test questionnaire focusing on prevention strategies and lead sources in rural children.Results: A total of 287 (96.6%) village doctors completed the survey in full. Most village doctors had an appropriate degree of general knowledge of lead poisoning; however, they had relatively poor knowledge of lead sources and prevention measures. Village doctors with an undergraduate level education scored an average of 2.7 points higher than those who had a junior college level education (p = 0.033). Village doctors with an annual income ≤ 10,000 RMB yuan scored 1.03 points lower than those whose income was >10,001 RMB yuan. Ethnic Han village doctors scored 1.12 points higher, on average, than ethnic Tujia village doctors (p = 0.027).Conclusions: This study identified important gaps in knowledge concerning lead poisoning in children among a rural population of village doctors. There is a clear need for multifaceted interventions that target village doctors to improve their knowledge regarding lead poisoning in children. The "What do you know" questionnaire is a new tool to evaluate lead poisoning knowledge and education projects. [ABSTRACT FROM AUTHOR]- Published
- 2017
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50. NLRP3 inflammasome activation promotes inflammation-induced carcinogenesis in head and neck squamous cell carcinoma.
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Cong-Fa Huang, Lei Chen, Yi-Cun Li, Lei Wu, Guang-Tao Yu, Wen-Feng Zhang, and Zhi-Jun Sun
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MELANOMA , *METASTASIS , *PROTEIN kinases , *CYCLOOXYGENASE 2 , *CELECOXIB , *POLYMERIZATION , *CELL migration - Abstract
Background: NLRP3 inflammasome acts as a danger signal sensor that triggers and coordinates the inflammatory response. However, the roles of NLRP3 inflammasome in the tumorigenesis and development of cancer stem cells (CSCs) of squamous cell carcinoma of the head and neck (SCCHN) remain ambiguous. Methods: In our study, tissue microarrays, ELISA, sphere-forming assay, colony formation assay and Western blot analysis were performed to evaluate the effect of NLRP3 inflammasome on the development of CSCs in human SCCHN tissue specimen, cell lines, and transgenic mouse SCCHN model. Results: The components of NLRP3 inflammasome, namely, NLRP3, ASC, Caspase-1, and IL-18 were correlated with CSCs markers BMI1, ALDH1 and CD44 in human SCCHN specimens. Moreover, NLRP3, Caspase-1, IL-1β, and IL-18 were highly expressed in SCCHN cell lines. NLRP3 inflammasome activated by LPS and ATP promoted sphere-forming and colony formation capacities along with an upregulation of BMI1, ALDH1 and CD44. In addition, NLRP3 inflammasome blockade by NLRP3 inhibitor MCC950 reduced sphere and colony number, also decreased the expression of BMI1, ALDH1 and CD44 in SCCHN cell lines. Expression of NLRP3, ASC, Caspase-1, IL-1β, IL-18, BMI1, ALDH1 and CD44 was upregulated in Tgfbr1/Pten 2cKO mouse SCCHN model, and NLRP3 inflammasome expression was closely related to those CSCs makers in mice SCCHN. However, MCC950 treatment reduced the expression of NLRP3 inflammasome, CSCs markers BMI1, ALDH1 and CD44 in Tgfbr1/Pten 2cKO mice SCCHN. In addition, blockade of NLRP3 inflammasome can also delayed the tumor-burdened speed in SCCHN mice. Conclusions: Our study demonstrates that NLRP3 inflammasome was upregulated and associated with the carcinogenesis and CSCs self-renewal activation in SCCHN. NLRP3 inflammasome can be a potential target in the development of novel approaches for head and neck squamous cell carcinoma therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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