4 results on '"K. Buschmann"'
Search Results
2. In-vitro examination of the positive inotropic effect of caffeine and taurine, the two most frequent active ingredients of energy drinks.
- Author
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Chaban R, Kornberger A, Branski N, Buschmann K, Stumpf N, Beiras-Fernandez A, and Vahl CF
- Subjects
- Aged, Atrial Appendage physiopathology, Cardiac Pacing, Artificial, Humans, In Vitro Techniques, Middle Aged, Time Factors, Atrial Appendage drug effects, Caffeine pharmacology, Cardiotonic Agents pharmacology, Energy Drinks, Myocardial Contraction drug effects, Taurine pharmacology
- Abstract
Background: Our study aimed to evaluate changes in the contractile behavior of human myocardium after exposure to caffeine and taurine, the main active ingredients of energy drinks (EDs), and to evaluate whether taurine exhibits any inotropic effect at all in the dosages commonly used in EDs., Methods: Myocardial tissue was removed from the right atrial appendages of patients undergoing cardiac surgery and prepared to obtain specimens measuring 4 mm in length. A total of 92 specimens were exposed to electrical impulses at a frequency of 75 bpm for at least 40 min to elicit their maximum contractile force before measuring the isometric contractile force (ICF) and duration of contraction (CD). Following this, each specimen was treated with either taurine (group 1, n = 29), or caffeine (group 2, n = 31) or both (group 3, n = 32). After exposure, ICF and CD measuring were repeated. Post-treatment values were compared with pre-treatments values and indicated as percentages., Results: Exposure to taurine did not alter the contraction behavior of the specimens. Exposure to caffeine, in contrast, led to a significant increase in ICF (118 ± 03%, p < 0.01) und a marginal decrease in CD (95 ± 1.6%, p < 0.01). Exposure to a combination of caffeine and taurine also induced a statistically significant increase in ICF (124 ± 4%, p < 0.01) and a subtle reduction in CD (92 ± 1.4%, p < 0.01). The increase in ICF achieved by administration of caffeine was similar to that achieved by a combination of both caffeine and taurine (p = 0.2). The relative ICF levels achieved by administration of caffeine and a combination of taurine and caffeine, respectively, were both significantly higher (p < 0.01) than the ICF resulting from exposure to taurine only., Conclusion: While caffeine altered the contraction behavior of the specimen significantly in our in-vitro model, taurine did not exhibit a significant effect. Adding taurine to caffeine did not significantly enhance or reduce the effect of caffeine.
- Published
- 2017
- Full Text
- View/download PDF
3. RAGE controls leukocyte adhesion in preterm and term infants.
- Author
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Buschmann K, Tschada R, Metzger MS, Braach N, Kuss N, Hudalla H, Poeschl J, and Frommhold D
- Subjects
- Adult, Cell Adhesion immunology, Female, Gene Expression Regulation immunology, Humans, Infant, Extremely Premature immunology, Infant, Newborn, Intercellular Adhesion Molecule-1 blood, Intercellular Adhesion Molecule-1 immunology, Leukocytes immunology, Leukocytes pathology, Lymphocyte Function-Associated Antigen-1 blood, Lymphocyte Function-Associated Antigen-1 immunology, Macrophage-1 Antigen blood, Macrophage-1 Antigen immunology, Male, Receptor for Advanced Glycation End Products, Receptors, Immunologic immunology, Infant, Extremely Premature blood, Leukocytes metabolism, Receptors, Immunologic blood
- Abstract
Background: Insufficient leukocyte recruitment may be one reason for the high incidence of life-threatening infections in preterm infants. Since the receptor of advanced glycation end products (RAGE) is a known leukocyte adhesion molecule and highly expressed during early development, we asked whether RAGE plays a role for leukocyte recruitment in preterm and term infants., Methods: Leukocyte adhesion was analyzed in dynamic flow chamber experiments using isolated leukocytes of cord blood from extremely premature (<30 weeks of gestation), moderately premature (30-35 weeks of gestation) and mature neonates (>35 weeks of gestation) and compared to the results of adults. For fluorescent microscopy leukocytes were labeled with rhodamine 6G. In the respective age groups we also measured the plasma concentration of soluble RAGE (sRAGE) by ELISA and Mac-1 and LFA-1 expression on neutrophils by flow cytometry., Results: The adhesive functions of fetal leukocytes significantly increase with gestational age. In all age groups, leukocyte adhesion was crucially dependent on RAGE. In particular, RAGE was equally effective to mediate leukocyte adhesion when compared to ICAM-1. The plasma levels of sRAGE were high in extremely premature infants and decreased with increasing gestational age. In contrast, expression of β2-Integrins Mac-1 and LFA-1 which are known ligands for RAGE and ICAM-1 did not change during fetal development., Conclusion: We conclude that RAGE is a crucial leukocyte adhesion molecule in both preterm and term infants.
- Published
- 2014
- Full Text
- View/download PDF
4. RAGE and ICAM-1 differentially control leukocyte recruitment during acute inflammation in a stimulus-dependent manner.
- Author
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Frommhold D, Kamphues A, Dannenberg S, Buschmann K, Zablotskaya V, Tschada R, Lange-Sperandio B, Nawroth PP, Poeschl J, Bierhaus A, and Sperandio M
- Subjects
- Animals, Cell Adhesion genetics, Cell Adhesion immunology, Cells, Cultured, Chemokine CXCL1 metabolism, Inflammation, Intercellular Adhesion Molecule-1 genetics, Intercellular Adhesion Molecule-1 immunology, Leukocytes immunology, Leukocytes pathology, Lymphocyte Function-Associated Antigen-1 immunology, Macrophage-1 Antigen immunology, Macrophage-1 Antigen metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Muscles pathology, N-Formylmethionine Leucyl-Phenylalanine metabolism, Protein Binding genetics, Protein Binding immunology, Receptor for Advanced Glycation End Products, Receptors, Immunologic genetics, Tumor Necrosis Factor-alpha immunology, Tumor Necrosis Factor-alpha metabolism, Venules pathology, Intercellular Adhesion Molecule-1 metabolism, Leukocytes metabolism, Lymphocyte Function-Associated Antigen-1 metabolism, Receptors, Immunologic metabolism, Venules metabolism
- Abstract
Background: The receptor for advanced glycation endproducts, RAGE, is involved in the pathogenesis of many inflammatory conditions, which is mostly related to its strong activation of NF-κB but also due to its function as ligand for the β2-integrin Mac-1. To further dissect the stimulus-dependent role of RAGE on leukocyte recruitment during inflammation, we investigated β2-integrin-dependent leukocyte adhesion in RAGE-/- and Icam1-/- mice in different cremaster muscle models of inflammation using intravital microscopy., Results: We demonstrate that RAGE, but not ICAM-1 substantially contributes to N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced leukocyte adhesion in TNF-α-pretreated cremaster muscle venules in a Mac-1-dependent manner. In contrast, fMLP-stimulated leukocyte adhesion in unstimulated cremaster muscle venules is independent of RAGE, but dependent on ICAM-1 and its interaction with LFA-1. Furthermore, chemokine CXCL1-stimulated leukocyte adhesion in surgically prepared cremaster muscle venules was independent of RAGE but strongly dependent on ICAM-1 and LFA-1 suggesting a differential and stimulus-dependent regulation of leukocyte adhesion during inflammation in vivo., Conclusion: Our results demonstrate that RAGE and ICAM-1 differentially regulate leukocyte adhesion in vivo in a stimulus-dependent manner.
- Published
- 2011
- Full Text
- View/download PDF
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