1. Herbal formula Xian-Fang-Huo-Ming-Yin regulates differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis mice
- Author
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Dashuai Zhu, Xue Li, Bo Nie, Yongzhe Che, Mingwei Mu, Yi Wei, Limin Chai, Lixia Lou, Bin Dong, Jingwei Zhou, Lingqun Zhu, Aiming Wu, Meng Chen, and Jinyu Li
- Subjects
0301 basic medicine ,Male ,CD3 ,Lymphocyte ,Arthritis ,Inflammation ,Proinflammatory cytokine ,03 medical and health sciences ,Mice ,medicine ,Animals ,Humans ,Lymphocytes ,Leflunomide ,Xian-fang-huo-ming-Yin (XFHM) ,Janus Kinases ,Nuclear factor κB (NF-κB) ,biology ,business.industry ,Collagen-induced arthritis (CIA) ,NF-kappa B ,Cell Differentiation ,General Medicine ,Pro-inflammatory cytokine ,medicine.disease ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Complementary and alternative medicine ,Mice, Inbred DBA ,Janus-activated kinase-signal transducer and activator of transcription (JAK/STAT) ,Immunology ,Cancer research ,biology.protein ,Cytokines ,Collagen ,Signal transduction ,medicine.symptom ,Janus kinase ,business ,medicine.drug ,Research Article ,Drugs, Chinese Herbal - Abstract
Background Xian-Fang-Huo-Ming-Yin (XFHM), a traditional herbal formula, has been used to treat sores and carbuncles for hundreds of years in Asia. Nowadays, its clinical effects in treatment of rheumatoid arthritis (RA) have been validated. In this study, we want to study its possible molecular mechanisms of regulating the differentiation of lymphocytes and production of pro-inflammatory cytokines in collagen-induced arthritis (CIA) mice for RA treatment. Methods A high performance liquid chromatography-electrospray ionization/mass spectrometer (HPLC-ESI/MSn) system was used to analyze the constituents of XFHM granules. An arthritics mouse model was induced by collagen and leflunomide (LEF) was used as a positive control medicine. Pathological changes at the metatarsophalangeal joint were studied through Safranin O and immunohistochemical staining. The differentiation of T, B and NK cells was examined by flow cytometry and pro-inflammatory cytokines were assayed using an Inflammation Antibody Array assay. The expression of key molecules of the nuclear factor κB (NF-κB) and Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathways in spleen were studied by western-blot analysis. Results In our study. 21 different dominant chemical constituents were identified in XFHM. Treatment with XFHM suppressed the pathological changes in arthrosis of CIA. Additionally, XFHM down-regulated the proliferation and differentiation of CD3+ T cells and CD3−CD19+ B cells significantly. However, XFHM had no significant effect on CD3−NK1.1+ NK cells. Further study showed that the production of pro-inflammatory cytokines had been suppressed by inhibiting the activation of NF-κB and JAK/STAT signaling. Conclusions XFHM can regulate and maintain the immunologic balance of lymphocytic immunity and inhibit the production of pro-inflammatory cytokines, thus suppressing the pathological changes of RA. Therefore, XFHM may be used as an application of traditional medicine against RA in modern complementary and alternative therapeutics. Electronic supplementary material The online version of this article (doi:10.1186/s12906-016-1526-x) contains supplementary material, which is available to authorized users.
- Published
- 2017