35 results on '"Jin Zhou"'
Search Results
2. Enhanced cellular therapy: revolutionizing adoptive cellular therapy
- Author
-
Xu, Meng-Yao, Zeng, Na, Liu, Chen-Qian, Sun, Jian-Xuan, An, Ye, Zhang, Si-Han, Xu, Jin-Zhou, Zhong, Xing-Yu, Ma, Si-Yang, He, Hao-Dong, Hu, Jia, Xia, Qi-Dong, and Wang, Shao-Gang
- Published
- 2024
- Full Text
- View/download PDF
3. Photodynamic therapy upregulates expression of HIF-1α and PD-L1 in related pathways and its clinical relevance in non-small-cell lung cancer
- Author
-
Sun, Wen, Cheng, Yuan, Ma, Xiaoyu, Jin, Zhou, Zhang, Qi, and Wang, Guangfa
- Published
- 2024
- Full Text
- View/download PDF
4. The moderated-mediation role of risk perception and intolerance of uncertainty in the association between residual symptoms and psychological distress: a cross-sectional study after COVID-19 policy lifted in China
- Author
-
Sun, Zhiyu, Jin, Zhou, Zhao, Kejie, Wen, Xin, Lu, Hui, Hu, Nuonuo, Zhu, Qinxin, Zhang, Yi, Ye, Minjie, Huang, Yili, Song, Weihong, Wang, Deborah Baofeng, and Wu, Yili
- Published
- 2024
- Full Text
- View/download PDF
5. Perceived risk of COVID-19 hurts mental health: the mediating role of fear of COVID-19 and the moderating role of resilience
- Author
-
Lu, Hui, Yang, Jialin, Zhao, Kejie, Jin, Zhou, Wen, Xin, Hu, Nuonuo, Yang, Hongshen, Sun, Zhiyu, Chen, Haitao, Huang, Yili, Wang, Deborah Baofeng, and Wu, Yili
- Published
- 2024
- Full Text
- View/download PDF
6. COVID-19 vaccine uptake and vaccine hesitancy in rural-to-urban migrant workers at the first round of COVID-19 vaccination in China
- Author
-
Hong, Liuzhi, Jin, Zhou, Xu, Kewei, Shen, Guanghui, Zou, Yang, Li, Ran, Xu, Lu, Wang, Dexuan, Chen, Li, Wu, Yili, and Song, Weihong
- Published
- 2023
- Full Text
- View/download PDF
7. ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma
- Author
-
Liu, Zhen-Yuan, Lan, Tian, Tang, Feng, He, Yong-Ze, Liu, Jin-Sheng, Yang, Jin-Zhou, Chen, Xi, Wang, Ze-Fen, and Li, Zhi-Qiang
- Published
- 2023
- Full Text
- View/download PDF
8. Perceived parenting styles and incidence of major depressive disorder: results from a 6985 freshmen cohort study
- Author
-
Cheng, Jing, Liu, Debiao, Zheng, Huancheng, Jin, Zhou, Wang, Deborah Baofeng, Liu, Yan, and Wu, Yili
- Published
- 2023
- Full Text
- View/download PDF
9. The renal pelvis urobiome in the unilateral kidney stone patients revealed by 2bRAD-M
- Author
-
Hong, Sen-Yuan, Yang, Yuan-Yuan, Xu, Jin-Zhou, Xia, Qi-Dong, Wang, Shao-Gang, and Xun, Yang
- Published
- 2022
- Full Text
- View/download PDF
10. Identification of the pivotal role of SPP1 in kidney stone disease based on multiple bioinformatics analysis
- Author
-
Hong, Sen-Yuan, Xia, Qi-Dong, Xu, Jin-Zhou, Liu, Chen-Qian, Sun, Jian-Xuan, Xun, Yang, and Wang, Shao-Gang
- Published
- 2022
- Full Text
- View/download PDF
11. Using circulating O-sulfotyrosine in the differential diagnosis of acute kidney injury and chronic kidney disease
- Author
-
Chen, Shuai, Liu, Yong-Hua, Dai, Dao-Peng, Zhu, Zheng-Bin, Dai, Yang, Wu, Zhi-Ming, Zhang, Li-Ping, Duan, Zhi-Feng, Lu, Lin, Ding, Feng-Hua, Zhu, Jin-Zhou, and Zhang, Rui-Yan
- Published
- 2021
- Full Text
- View/download PDF
12. Effect of tumour size ratio on liver recurrence-free survival of patients undergoing hepatic resection for colorectal liver metastases
- Author
-
Yana Zhai, Jingdong Zhang, Weijun Bai, Qian Dong, and Jin Zhou
- Subjects
Male ,medicine.medical_specialty ,Cancer Research ,genetic structures ,Hepatic resection ,Tumour size ratio ,Liver recurrence-free survival ,Gastroenterology ,Disease-Free Survival ,Risk Factors ,Internal medicine ,Recurrence free survival ,medicine ,Genetics ,Hepatectomy ,Humans ,RC254-282 ,Proportional Hazards Models ,Retrospective Studies ,business.industry ,fungi ,Liver Neoplasms ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Middle Aged ,Prognosis ,Survival Analysis ,Tumor Burden ,Colorectal liver metastases ,Treatment Outcome ,Oncology ,Liver ,Tumour size ,Female ,Tumour burden score ,Neoplasm Recurrence, Local ,business ,Colorectal Neoplasms ,Research Article - Abstract
Background The study aimed to assess the impact of size differences of multiple liver metastases on liver recurrence-free survival (RFS) in patients undergoing hepatic resection for colorectal liver metastases (CRLMs). Methods Overall, 147 patients with CRLMs who underwent hepatic resection between January 2010 and December 2016 were retrospectively analysed. Tumour size ratio (TSR) was defined as the maximum diameter of the largest liver lesion over the maximum diameter of the smallest liver lesion. The univariate and multivariate analyses were performed to determine independent prognostic risk factors. The prognostic value of the TSR was further explored in each Tumour Burden Score (TBS) zone. Log-rank survival analyses were used to compare liver RFS in the new clinical score and the Fong clinical score. Results Based on the TSR, patients were classified into three groups: TSR P = 0.003; zone 2: median liver RFS, 3.5, 5.0, and 10.9 months for groups 1, 2, and 3, respectively, P Conclusions TSR, as a prognostic tool, could accurately assess the effect of size differences across multiple liver metastases on liver RFS in patients undergoing hepatectomy for CRLMs. Trial registration Retrospectively registered
- Published
- 2022
13. Synapse-specific Lrp4 mRNA enrichment requires Lrp4/MuSK signaling, muscle activity and Wnt non-canonical pathway
- Author
-
Dongyan Ren, Zheng Yu, Erkang Fei, Shunqi Wang, Xinsheng Lai, Baoming Li, Jin Zhou, Hongyang Jing, Peng Chen, and Tiankun Hui
- Subjects
Cell signaling ,Wnt non-canonical signaling ,QH301-705.5 ,Lrp4 ,Neuromuscular junction ,QD415-436 ,Biochemistry ,General Biochemistry, Genetics and Molecular Biology ,Synapse ,medicine ,β-gal ,Biology (General) ,Acetylcholine receptor ,Myogenesis ,Chemistry ,Research ,Wnt signaling pathway ,Cell biology ,medicine.anatomical_structure ,medicine.symptom ,C2C12 ,TP248.13-248.65 ,Biotechnology ,Muscle contraction ,Muscle activity - Abstract
Background The neuromuscular junction (NMJ) is a peripheral synapse critical to muscle contraction. Like acetylcholine receptors (AChRs), many essential proteins of NMJ are extremely concentrated at the postjunctional membrane. However, the mechanisms of synapse-specific concentration are not well understood; furthermore, it is unclear whether signaling molecules critical to NMJ formation and maintenance are also locally transcribed. Results We studied the β-gal activity encoded by a lacZ cassette driven by the promoter of the Lrp4 gene. As reported for Lrp4 mRNA, β-gal was in the central region in embryonic muscles and at the NMJ after its formation. However, β-gal was no longer in the central areas of muscle fibers in Lrp4 or MuSK mutant mice, indicating a requirement of Lrp4/MuSK signaling. This phenotype could be rescued by transgenic expression of LRP4 with a transmembrane domain but not soluble ECD in Lrp4 mutant mice. β-gal and AChR clusters were distributed in a broader region in lacZ/ECD than that of heterozygous lacZ/+ mice, indicating an important role of the transmembrane domain in Lrp4 signaling. Synaptic β-gal activity became diffused after denervation or treatment with µ-conotoxin, despite its mRNA was increased, indicating synaptic Lrp4 mRNA enrichment requires muscle activity. β-gal was also diffused in aged mice but became re-concentrated after muscle stimulation. Finally, Lrp4 mRNA was increased in C2C12 myotubes by Wnt ligands in a manner that could be inhibited by RKI-1447, an inhibitor of ROCK in Wnt non-canonical signaling. Injecting RKI-1447 into muscles of adult mice diminished Lrp4 synaptic expression. Conclusions This study demonstrates that synapse-specific enrichment of Lrp4 mRNA requires a coordinated interaction between Lrp4/MuSK signaling, muscle activity, and Wnt non-canonical signaling. Thus, the study provides a new mechanism for Lrp4 mRNA enrichment. It also provides a potential target for the treatment of NMJ aging and other NMJ-related diseases.
- Published
- 2021
14. In vitro study on the effect of cornin on the activity of cytochrome P450 enzymes
- Author
-
Xuedong Zhou, Qun Zhang, Zengqiang Qu, Yanqing Zhou, Qiuping Xu, Shengjian Li, Jin Zhou, and Junwei Yang
- Subjects
Gene isoform ,CYP2C9 ,CYP3A4 ,Phytochemicals ,Pharmacology ,Cornin ,030226 pharmacology & pharmacy ,Other systems of medicine ,03 medical and health sciences ,0302 clinical medicine ,Cytochrome P-450 Enzyme System ,Cytochrome P-450 Enzyme Inhibitors ,Humans ,CYP2E1 ,chemistry.chemical_classification ,biology ,Research ,CYP1A2 ,Cytochrome P450 ,Verbena ,Kinetics ,Enzyme ,Complementary and alternative medicine ,chemistry ,030220 oncology & carcinogenesis ,Iridoid Glycosides ,biology.protein ,Microsome ,Microsomes, Liver ,RZ201-999 ,Drug-drug interaction - Abstract
BackgroundCornin is a commonly used herb in cardiology for its cardioprotective effect. The effect of herbs on the activity of cytochrome P450 enzymes (CYP450s) can induce adverse drug-drug interaction even treatment failure. Therefore, it is necessary to investigate the effect of cornin on the activity of CYP450s, which can provide more guidance for the clinical application of cornin.MethodsCornin (100 μM) was incubated with eight isoforms of CYP450s, including CYP1A2, 2A6, 3A4, 2C8, 2C9, 2C19, 2D6, and 2E1, in pooled human liver microsomes. The inhibition model and corresponding parameters were also investigated.ResultsCornin exerted significant inhibitory effect on the activity of CYP3A4, 2C9, and 2E1 in a dose-dependent manner with the IC50values of 9.20, 22.91, and 14.28 μM, respectively (p Kivalue of 4.69 μM, while the inhibition of CYP2C9 and 2E1 by cornin was competitive with theKivalue of 11.31 and 6.54 μM, respectively. Additionally, the inhibition of CYP3A4 by cornin was found to be time-dependent with theKI/Kinactvalue of 6.40/0.055 min− 1·μM− 1.ConclusionsThe inhibitory effect of cornin on the activity of CYP3A4, 2C9, and 2E1 indicated the potential drug-drug interaction between cornin and drugs metabolized by these CYP450s, which needs further investigation and validation.
- Published
- 2021
15. E. coli diversity: low in colorectal cancer
- Author
-
Zheng Liu, Zhou Yujie, Dan-Dan Zhao, Jia-Jing Li, Randal N. Johnston, Gui-Rong Liu, Li Wang, Wei-Qiao Liu, Jing-Bo Zhao, Xia Li, Songling Zhu, Yong-Guo Li, Gong-Da Liang, Chun-Xiao Wang, Man-Fei Zhao, Shu-Lin Liu, Zhi-Rong Guo, Xiaoyun Chang, Hai-Ning Li, Li-Bo Duo, He Zhuang, Le Tang, Jin Zhou, Xiao-Yan He, Bing-Qing Yao, and Xiao-Qin Mu
- Subjects
0301 basic medicine ,Adult ,DNA, Bacterial ,Male ,lcsh:Internal medicine ,China ,lcsh:QH426-470 ,Intestinal microbiota ,Adolescent ,Genotype ,030106 microbiology ,Longevity ,medicine.disease_cause ,Genome ,Genetic diversity ,03 medical and health sciences ,Young Adult ,Genetics ,medicine ,Pulsed-field gel electrophoresis ,Escherichia coli ,Tumor Microenvironment ,Humans ,lcsh:RC31-1245 ,Child ,Genetics (clinical) ,Escherichia coli Infections ,Phylogeny ,Aged ,biology ,Host (biology) ,Genetic Variation ,Middle Aged ,biology.organism_classification ,Commensalism ,Colorectal cancer ,lcsh:Genetics ,030104 developmental biology ,Child, Preschool ,Female ,Colorectal Neoplasms ,Bacteria ,Research Article - Abstract
Background Escherichia coli are mostly commensals but also contain pathogenic lineages. It is largely unclear whether the commensal E. coli as the potential origins of pathogenic lineages may consist of monophyletic or polyphyletic populations, elucidation of which is expected to lead to novel insights into the associations of E. coli diversity with human health and diseases. Methods Using genomic sequencing and pulsed field gel electrophoresis (PFGE) techniques, we analyzed E. coli from the intestinal microbiota of three groups of healthy individuals, including preschool children, university students, and seniors of a longevity village, as well as colorectal cancer (CRC) patients, to probe the commensal E. coli populations for their diversity. Results We delineated the 2280 fresh E. coli isolates from 185 subjects into distinct genome types (genotypes) by PFGE. The genomic diversity of the sampled E. coli populations was so high that a given subject may have multiple genotypes of E. coli, with the general diversity within a host going up from preschool children through university students to seniors. Compared to the healthy subjects, the CRC patients had the lowest diversity level among their E. coli isolates. Notably, E. coli isolates from CRC patients could suppress the growth of E. coli bacteria isolated from healthy controls under nutrient-limited culture conditions. Conclusions The coexistence of multiple E. coli lineages in a host may help create and maintain a microbial environment that is beneficial to the host. As such, the low diversity of E. coli bacteria may be associated with unhealthy microenvironment in the intestine and hence facilitate the pathogenesis of diseases such as CRC.
- Published
- 2020
16. Comparison of the retroperitoneal versus Transperitoneal laparoscopic Adrenalectomy perioperative outcomes and safety for Pheochromocytoma: a meta-analysis
- Author
-
Kang-Er Wang, Chun-Hua Ye, Xie-Lai Zhou, Zhen Li, Lu-Jie Qian, Yu-Li Jiang, and Jin Zhou
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,Operative Time ,lcsh:Surgery ,Adrenal Gland Neoplasms ,Blood Loss, Surgical ,Hemodynamics ,Pheochromocytoma ,03 medical and health sciences ,0302 clinical medicine ,Laparoscopic ,medicine ,Humans ,Retroperitoneal Space ,Laparoscopic adrenalectomy ,business.industry ,Incidence (epidemiology) ,Adrenalectomy ,Retrospective cohort study ,Transperitoneal ,lcsh:RD1-811 ,General Medicine ,Perioperative ,Retroperitoneal ,Length of Stay ,medicine.disease ,Surgery ,Meta-analysis ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Laparoscopy ,business ,Research Article - Abstract
Background To compare the perioperative outcomes and safety of transperitoneal laparoscopic adrenalectomy with those of retroperitoneal laparoscopic adrenalectomy for patients with pheochromocytoma. Methods We searched PubMed, EMBASE and the Cochrane Central Register for studies from 1999 to 2019 to assess the perioperative outcomes and safety of transperitoneal laparoscopic adrenalectomy and the retroperitoneal approach for laparoscopic adrenalectomy in patients with pheochromocytoma. After data extraction and quality assessments, we used RevMan 5.2 to pool the data. Results Four retrospective studies were obtained in our meta-analysis. Patients who underwent retroperitoneal laparoscopic adrenalectomy were associated with shorter operative time (WMD: 34.91, 95% CI: 27.02 to 42.80, I2 = 15%; p p p 2 = 0; p = 0.38) or in the incidence of hemodynamic crisis (OR: 0.74, 95% CI: 0.19 to 2.94, p = 0.67) between the two groups. Conclusion Retroperitoneal laparoscopic adrenalectomy could achieve better perioperative outcomes than the transperitoneal approach for patients with pheochromocytoma.
- Published
- 2020
17. Testing rare variants for hypertension using family-based tests with different weighting schemes
- Author
-
Xingwang Zhao, Xuexia Wang, and Jin Zhou
- Subjects
0301 basic medicine ,education.field_of_study ,Population ,General Medicine ,Computational biology ,Biology ,computer.software_genre ,Rare variant association ,General Biochemistry, Genetics and Molecular Biology ,Weighting ,03 medical and health sciences ,030104 developmental biology ,Proceedings ,Nonsynonymous snps ,Allelic heterogeneity ,Data mining ,education ,Family based ,computer - Abstract
Next-generation sequencing technology makes directly testing rare variants possible. However, existing statistical methods to detect common variants may not be optimal for testing rare variants because of allelic heterogeneity as well as the extreme rarity of individual variants. Recently, several statistical methods to detect associations of rare variants were developed, including population-based and family-based methods. Compared with population-based methods, family-based methods have more power and can prevent bias induced by population substructure. Both population-based and family-based methods for rare variant association studies are essentially testing the effect of a weighted combination of variants or its function. How to model the weights is critical for the testing power because the number of observations for any given rare variant is small and the multiple-test correction is more stringent for rare variants. We propose 4 weighting schemes for the family-based rare variants test (FBAT-v) to test for the effects of both rare and common variants across the genome. Applying FBAT-v with the proposed weighting schemes on the Genetic Analysis Workshop 19 family data indicates that the power of FBAT-v can be comparatively enhanced in most circumstances.
- Published
- 2016
18. The roles of nuclear focal adhesion kinase (FAK) on Cancer: a focused review
- Author
-
Jin Zhou, Liling Tang, and Qian Yi
- Subjects
0301 basic medicine ,Cancer Research ,Cytoplasm ,Integrin ,Antineoplastic Agents ,Review ,lcsh:RC254-282 ,Focal adhesion ,03 medical and health sciences ,0302 clinical medicine ,Growth factor receptor ,Cell Line, Tumor ,Neoplasms ,Transcription factors ,Animals ,Humans ,Molecular Targeted Therapy ,Transcription factor ,Protein Kinase Inhibitors ,Cancer ,Cell Proliferation ,Cell Nucleus ,Tumor microenvironment ,biology ,Kinase ,Chemistry ,Inhibitors ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Cell biology ,Nuclear FAK ,Protein Transport ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Focal Adhesion Protein-Tyrosine Kinases ,Cancer cell ,biology.protein ,Gene expression ,biological phenomena, cell phenomena, and immunity ,Tyrosine kinase ,Signal Transduction - Abstract
FAK is a tyrosine kinase overexpressed in cancer cells and plays an important role in the progression of tumors to a malignant phenotype. Except for its typical role as a cytoplasmic kinase downstream of integrin and growth factor receptor signaling, related studies have shown new aspects of the roles of FAK in the nucleus. FAK can promote p53 degradation through ubiquitination, leading to cancer cell growth and proliferation. FAK can also regulate GATA4 and IL-33 expression, resulting in reduced inflammatory responses and immune escape. These findings establish a new model of FAK from the cytoplasm to the nucleus. Activated FAK binds to transcription factors and regulates gene expression. Inactive FAK synergizes with different E3 ligases to promote the turnover of transcription factors by enhancing ubiquitination. In the tumor microenvironment, nuclear FAK can regulate the formation of new blood vessels, affecting the tumor blood supply. This article reviews the roles of nuclear FAK in regulating gene expression. In addition, the use of FAK inhibitors to target nuclear FAK functions will also be emphasized.
- Published
- 2019
19. Validity and reliability of the EQ-5D-5 L in family caregivers of leukemia patients
- Author
-
Chaojie Liu, Limin Li, Erwei Zheng, Jin Zhou, Xueyun Zeng, Jiao Xu, Mingjie Sui, Hongjuan Yu, Yang Li, Weidong Huang, and Xiuzhi Cai
- Subjects
0301 basic medicine ,Adult ,Male ,Cancer Research ,China ,Psychometrics ,Validity ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,Quality of life ,EQ-5D ,Surveys and Questionnaires ,Genetics ,medicine ,Health Status Indicators ,Humans ,Reliability (statistics) ,Leukemia ,Family caregivers ,Discriminant validity ,Reproducibility of Results ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Reliability ,030104 developmental biology ,Cross-Sectional Studies ,Oncology ,Convergent validity ,Caregivers ,030220 oncology & carcinogenesis ,EQ-5D-5 L ,Quality of Life ,Anxiety ,Female ,medicine.symptom ,Family caregiver ,Psychology ,Clinical psychology ,Research Article - Abstract
Purpose This study aimed to test the validity and reliability of the five-level EuroQol five-dimensional (EQ-5D-5 L) instrument in family caregivers (FCs) of leukemia patients in Heilongjiang of China. Methods A cross-sectional survey was conducted on 298 family caregivers (FCs) of leukemia patients from three major cancer centers in the capital city of Heilongjiang province of China. Their dimensional scores of the EQ-5D-5 L were compared with those of the WHOQOL-BREF to test the convergent validity (constructs measuring the same concept) and divergent validity (constructs measuring different concepts) of the EQ-5D-5 L. Repeated surveys were conducted on 271 participants to determine the test–retest reliability of the EQ-5D-5 L. Results The four physical dimensions (mobility, self-care, usual activities, and pain/discomfort) of the EQ-5D-5 L had moderate or high correlations with the physical health domain of the WHOQOL-BREF, with a correlation coefficient (r) ranging from 0.459 to 0.559. The anxiety/depression dimension of the EQ-5D-5 L had a high correlation (r = 0.667) with the psychological domain of the WHOQOL-BREF. By contrast, lower but still significant physical-to-psychological correlations were found between the two instruments (r ranging from 0.219 to 0.396). In addition, the EQ-5D-5 L dimensional scores showed no or weak correlations with the environment and social domains of the WHOQOL-BREF (r ranging from 0.016 to 0.207). High test-retest reliability (> 0.7) was evident. Conclusion The Chinese version of the EQ-5D-5 L has satisfactory reliability and validity in FCs of leukemia patients. It can be used to elicit utility of health-related quality of life in FCs of leukemia.
- Published
- 2019
20. Validation and comparison of EuroQoL-5 dimension (EQ-5D) and Short Form-6 dimension (SF-6D) among stable angina patients
- Author
-
He Sun, Fei-Li Zhao, Yuerong Han, Jin Zhou, Zhijun Chen, and Jing Wu
- Subjects
Gerontology ,Quality of life ,Male ,medicine.medical_specialty ,Stable angina ,China ,Multivariate analysis ,Psychometrics ,Economics ,Health Status ,Correlation ,Angina ,Utility ,Asian People ,EQ-5D ,Surveys and Questionnaires ,Medicine ,Health Status Indicators ,Humans ,Angina, Stable ,Physical Examination ,Aged ,Receiver operating characteristic ,business.industry ,Research ,Public Health, Environmental and Occupational Health ,Reproducibility of Results ,General Medicine ,Middle Aged ,medicine.disease ,Convergent validity ,ROC Curve ,Multivariate Analysis ,Physical therapy ,Linear Models ,Ceiling effect ,Female ,business ,SF-6D - Abstract
Objectives Several preference-based health-related quality of life (HRQoL) instruments have been published and widely used in different populations. However no consensus has emerged regarding the most appropriate instrument in therapeutic area of stable angina. This study compared and validated the psychometric properties of two generic preference-based instruments, the EQ-5D and SF-6D, among Chinese stable angina patients. Methods Convergent validity of the EQ-5D and SF-6D was examined with eight a priori hypotheses from stable angina patients in conjunction with Seattle Angina Questionnaire (SAQ). Responsiveness was compared using the effect size (ES), relative efficiency (RE) and receiver operating characteristic (ROC) curves. Agreement between the EQ-5D and SF-6D was tested using intra-class correlation coefficient (ICC) and Bland-Altman plot. Factors affecting utility difference were explored with multiple linear regression analysis. Results In 411 patients (mean age 68.08 ± 11.35), mean utility scores (SD) were 0.78 (0.15) for the EQ-5D and 0.68 (0.12) for the SF-6D. Validity was demonstrated by the moderate to strong correlation coefficients (Range: 0.368-0.594, P
- Published
- 2014
21. Complete genome sequence of Salmonella enterica subspecies arizonae str. RKS2983
- Author
-
Yong-Guo Li, Xiaoyu Wang, Randal N. Johnston, Shu-Lin Liu, Songling Zhu, Gui-Rong Liu, Jin Zhou, Bailiang Li, Chun-Xiao Wang, and Ye Feng
- Subjects
Whole genome sequencing ,Genetics ,Salmonella ,biology ,Facultative anaerobe ,Human pathogen ,medicine.disease_cause ,biology.organism_classification ,Genome ,Short Genome Report ,Genetic divergence ,Host-adapted ,Salmonella enterica ,S. enterica subspecies arizonae RKS2983 ,Salmonella pathogenicity islands ,medicine ,RRNA Operon ,Genomic evolution ,Gene - Abstract
Salmonella arizonae (also called Salmonella subgroup IIIa) is a Gram-negative, non-spore-forming, motile, rod-shaped, facultatively anaerobic bacterium. S. arizonae strain RKS2983 was isolated from a human in California, USA. S. arizonae lies somewhere between Salmonella subgroups I (human pathogens) and V (also called S. bongori; usually non-pathogenic to humans) and so is an ideal model organism for studies of bacterial evolution from non-human pathogen to human pathogens. We hence sequenced the genome of RKS2983 for clues of genomic events that might have led to the divergence and speciation of Salmonella into distinct lineages with diverse host ranges and pathogenic features. The 4,574,836 bp complete genome contains 4,203 protein-coding genes, 82 tRNA genes and 7 rRNA operons. This genome contains several characteristics not reported to date in Salmonella subgroup I or V and may provide information about the genetic divergence of Salmonella pathogens.
- Published
- 2015
22. Factors associated with the quality of life of family caregivers for leukemia patients in China.
- Author
-
Hongjuan Yu, Limin Li, Chaojie Liu, Weidong Huang, Jin Zhou, Wenqi Fu, Yi Ma, Si Li, Yuying Chang, Guoxiang Liu, Qunhong Wu, Yu, Hongjuan, Li, Limin, Liu, Chaojie, Huang, Weidong, Zhou, Jin, Fu, Wenqi, Ma, Yi, Li, Si, and Chang, Yuying
- Subjects
LEUKEMIA ,CAREGIVERS ,QUALITY of life ,REGRESSION analysis ,PSYCHOLOGICAL distress ,PATIENTS ,LEUKEMIA treatment ,PALLIATIVE treatment ,MENTAL health ,ADAPTABILITY (Personality) ,PSYCHOLOGY of caregivers ,HOME nursing ,QUESTIONNAIRES ,SOCIAL support ,CROSS-sectional method ,PSYCHOLOGY - Abstract
Background: The leukemia affects not only the quality of life (QOL) of patients with the disease but also that of their family caregivers (FCs). The research studies on QOL of FCs for leukemia patients are limited. This study aimed to evaluate the QOL of FCs for leukemia patients in Heilongjiang province, China.Methods: A cross-sectional questionnaire survey was undertaken with 309 FCs for leukemia patients recruited from three hospitals in Heilongjiang province. The QOL of the participants was assessed using the Chinese version of WHOQOL-BREF. Multivariate regression models were established to determine the predictors of the QOL of FCs, including the socio-economic characteristics of patients and FCs, and the emotional distress, social support and family functions of FCs.Results: The FCs had low QOL scores in all four domains: 12.7 ± 2.8 for physical, 12.2 ± 2.5 for psychological, 13.2 ± 2.9 for social and 11.3 ± 2.5 for environment. Social support is a major predictor of the QOL of FCs, with a standardized β coefficient of "high support" ranging from 0.41 to 0.58 for the four domains, followed by family function (β = 0.37 ~ 0.44 for psychological, social and environmental domains). The FCs who were older, highly educated, had no religious belief, suffered from a higher level of emotional distress, and provided care to younger patients and the patients without insurance coverage had lower QOL than the others.Conclusion: The study provides some important insights into the QOL of FCs for leukemia patients. The QOL of FCs for leukemia patients is low and low levels of support to FCs are a major predictor of low QOL of FCs. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
23. Grayscale inversion radiographic view provided improved intra- and interobserver reliabilities in measuring spinopelvic parameters in asymptomatic adult population.
- Author
-
Weixiang Sun, Jin Zhou, Xiaodong Qin, Leilei Xu, Xinxin Yuan, Yang Li, Yong Qiu, and Zezhang Zhu
- Subjects
- *
KYPHOSIS , *SCOLIOSIS , *SPINE abnormalities , *BONE diseases , *SACRUM diseases , *RADIOGRAPHY - Abstract
Background: Recently, a grayscale inversion view was reported to improve intra- and inter-observer reliabilities in measuring coronal curvature with Cobb and pedicle methods in scoliosis patients. However, the grayscale transformation has never been applied to the measurements of spinopelvic parameters. The purpose of this study was to compare the measurement reliabilities of the spinoplevic sagittal parameters between the 'Standard View' and the 'Grayscale Inversion View' in normal adult populations. Methods: A total of 30 asymptomatic subjects aged between 30 and 40 years were included in this study. Wholespine posteroanterior radiographs were used to measure the spinoplevic sagittal parameters including thoracic kyphosis (TK), lumbar lordosis (LL), sagittal vertical axis (SVA), pelvic incidence (PI), sacral slope (SS) and pelvic tilt (PT) in both standard view and grayscale inversion view. Two independent observers measured the parameters twice at a 2-week interval. Intra- and inter-observer reliabilities were compared between the two radiographic views. The absolute differences between the two sets of measurements on each view were calculated and compared. Results: The intra-class correlation coefficients (ICCs) of PI, PT and SVA were greater in the grayscale inversion view than in the standard view (0.972 vs 0.817, 0.937 vs 0.833 and 0.964 vs 0.901 for observer 1, respectively; 0.990 vs 0. 826, 0.995 vs 0.842 and 0.969 vs 0.919 for observer 2, respectively). Overall, the improvement of ICC was greater in parameters of sagittal pelvic alignment than in those of sagittal spinal alignment. As for the mean absolute differences between two measurements, significant differences existed between the two views in terms of PI, PT and SVA (p = 0.014, 0.016 and 0.011 for observer 1, respectively; p = 0.014, 0.025 and 0.046 for observer 2, respectively). Conclusions: A grayscale inversion view provided improved intra- and inter-observer reliabilities in measuring spinoplevic alignment when compared with a standard view. This view was more useful in subjects whose pelvic anatomical structures can't be identified clearly on the standard X-ray view. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
24. Cartilage matrix changes in contralateral mobile knees in a rabbit model of osteoarthritis induced by immobilization.
- Author
-
Qiang Zhou, Bo Wei, Shuai Liu, Fengyong Mao, Xiang Zhang, Jun Hu, Jin Zhou, Qingqiang Yao, Yan Xu, Liming Wang, Zhou, Qiang, Wei, Bo, Liu, Shuai, Mao, Fengyong, Zhang, Xiang, Hu, Jun, Zhou, Jin, Yao, Qingqiang, Xu, Yan, and Wang, Liming
- Subjects
ANIMAL experimentation ,ARTICULAR cartilage ,BIOLOGICAL models ,COLLAGEN ,GLYCOSAMINOGLYCANS ,IMMUNOHISTOCHEMISTRY ,KNEE ,KNEE diseases ,OSTEOARTHRITIS ,RABBITS ,DISEASE progression ,METABOLISM - Abstract
Background: Many researches have investigated the changes associated with immobilization-induced osteoarthritis (OA). However, there are only few studies focusing on the effect of unilateral knee immobilization on cartilage matrix changes in the contralateral mobile knee. The aim of the present study was to investigate the influence of immobilization on the cartilage matrix in the contralateral mobile knees in a rabbit model of OA induced by immobilization.Methods: Right knees (experimental knees) of eighteen mature female rabbits were immobilized at an extension of 180° with orthopedic casting tape for 2, 4, or 8 weeks. Left knees (contralateral knees) of the immobilized rabbits were not subjected to immobilization. The knees of six non-immobilized rabbits were designated as control knees. Following immobilization, cartilage specimens from the medial femoral condyle underwent macroscopic, histological, immunohistochemical, and biochemical evaluations.Results: Roughness of cartilage surface was detected in the experimental knees at 2 weeks, and cartilage degeneration was further developed. In the contralateral knee, cartilage showed degenerative changes after 4 weeks. Safranin-O staining and glycosaminoglycan (GAG) contents were reduced in the experimental knees following immobilization and in the contralateral intact knees after 4 and 8 weeks. Type II collagen staining was gradually reduced, type I collagen accumulation was obviously detected in the upper and middle layers of cartilage in experimental knees after 8 weeks, and the collagen orientation was gradually disorganized in both knees at 4 and 8 weeks. For both experimental and contralateral knees, collagen contents were significantly decreased at 8 weeks, and Mankin and Osteoarthritis Research Society International (OARSI) scores increased over time.Conclusion: OA developed in the contralateral intact knee with the progress of OA in the immobilized knee in a rabbit model of immobilization-induced OA. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF
25. FoxM1 transactivates PTTG1 and promotes colorectal cancer cell migration and invasion.
- Author
-
Yun Zheng, Jinjun Guo, Jin Zhou, Jinjian Lu, Qi Chen, Cui Zhang, Chen Qing, Koeffler, H. Philip, and Yunguang Tong
- Subjects
COLON cancer ,CANCER cell migration ,CANCER invasiveness ,METASTASIS ,CANCER-related mortality ,FORKHEAD transcription factors - Abstract
Background: Metastasis is the major cause of cancer-related death. Forkhead Box M1 (FoxM1) is a master regulator of tumor metastasis. This study aims to identify new FoxM1 targets in regulating tumor metastasis using bioinformatics tools as well as biological experiments. Methods: Illumina microarray was used to profile WT and PTTG1 knockout HCT116 cells. R2 Genomics Analysis was used to identify PTTG1 as a potential FoxM1 targeted gene. Luciferase reporter array, EMSA and Chromatin Immunoprecipitation (ChIP) were used to determine the binding of FoxM1 to PTTG1 promoter. Boyden chamber assay was used to evaluate the effects of FoxM1-PTTG1 on cell migration and invasion. Splenic-injection induced liver metastasis model was used to evaluate the effects of FoxM1-PTTG1 on liver metastasis of colorectal cancer. Results: Analyses of multiple microarray datasets derived from human colorectal cancer indicated that correlation levels of FoxM1 and pituitary tumor transforming gene (PTTG1) are highly concordant (R = 0.68 ~ 0.89, p = 2.1E-226 ~ 9.6E-86). FoxM1 over-expression increased and knock-down decreased PTTG1 expression. Luciferase reporter assay identified that the -600 to -300 bp region of PTTG1 promoter is important for FoxM1 to enhance PTTG1 promoter activity. EMSA and ChIP assays confirmed that FoxM1 directly binds to PTTG1 promoter at the -391 to -385 bp region in colorectal cancer cells. Boyden chamber assay indicated that both FoxM1 and PTTG1 regulate migration and invasion of HCT116 and SW620 colorectal cancer cells. Further in vivo assays indicated that PTTG1 knock out decreased the liver metastasis of FoxM1 over-expressing HCT116 cells. Microarray analyses identified 662 genes (FDR < 0.05) differentially expressed between WT and PTTG1
-/- HCT116 cells. Among them, dickkopf homolog 1 (DKK1), a known WNT pathway inhibitor, was suppressed by PTTG1 and FoxM1. Conclusions: PTTG1 is a FoxM1 targeted gene. FoxM1 binds to PTTG1 promoter to enhance PTTG1 transcription, and FoxM1-PTTG1 pathway promotes colorectal cancer migration and invasion. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF
26. Complete genome sequence of Salmonella enterica subspecies arizonae str. RKS2983.
- Author
-
Chun-Xiao Wang, Song-Ling Zhu, Xiao-Yu Wang, Ye Feng, Bailiang Li, Yong-Guo Li, Johnston, Randal N., Gui-Rong Liu, Jin Zhou, and Shu-Lin Liu
- Subjects
SALMONELLA enterica ,BACTERIAL genomes ,BACTERIAL evolution ,PATHOGENIC bacteria ,BACTERIAL genetics - Abstract
Salmonella arizonae (also called Salmonella subgroup IIIa) is a Gram-negative, non-spore-forming, motile, rod-shaped, facultatively anaerobic bacterium. S. arizonae strain RKS2983 was isolated from a human in California, USA. S. arizonae lies somewhere between Salmonella subgroups I (human pathogens) and V (also called S. bongori; usually non-pathogenic to humans) and so is an ideal model organism for studies of bacterial evolution from non-human pathogen to human pathogens. We hence sequenced the genome of RKS2983 for clues of genomic events that might have led to the divergence and speciation of Salmonella into distinct lineages with diverse host ranges and pathogenic features. The 4,574,836 bp complete genome contains 4,203 protein-coding genes, 82 tRNA genes and 7 rRNA operons. This genome contains several characteristics not reported to date in Salmonella subgroup I or V and may provide information about the genetic divergence of Salmonella pathogens. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
27. Neoadjuvant chemotherapy for primary adenocarcinomas of the urinary bladder: a single-site experience.
- Author
-
Bin Yu, Jin Zhou, Hongzhou Cai, Ting Xu, Zicheng Xu, Qing Zou, and Min Gu
- Subjects
BLADDER cancer treatment ,PHYSIOLOGICAL effects of chemotherapy ,CISPLATIN ,NUCLEOSIDES ,DRUG dosage ,THERAPEUTICS - Abstract
Background: Adenocarcinoma of the urinary bladder is a rare malignancy. Radical surgery is suggested as the best available treatment for early-stage disease, but there is currently no consensus on standard chemotherapy regimen for advanced stage. We assessed the feasibility and effect of neoadjuvant chemotherapy with gemcitabine and cisplatin (GC) plus S-1 for patients with locally advanced primary adenocarcinomas of the urinary bladder. Methods: Six patients with locally advanced urachal or non-urachal (n = 3, each) primary adenocarcinoma of the bladder were treated from October 2010 to October 2013 at a single center. All the patients were treated with 3 cycles (21d, each) of GC plus S-1 (gemcitabine, 1000 mg/m², days 1 and 8; cisplatin, 70 mg/m², day 2; and S-1, 50 mg bid, day 1-14). After neoadjuvant chemotherapy, patients with urachal cancer were treated with en bloc radical cystectomy and umbilectomy; the remaining 3 patients were treated with cystectomy. Results: All patients successfully completed the neoadjuvant chemotherapy without serious side effects. Two patients were assessed as complete response, 2 as partial response, 1 as stable disease and 1 as progressive disease. Conclusions: Despite the limitations of a small study population, the GC plus S-1 regimen for locally advanced primary adenocarcinoma of the urinary bladder was effective, and facilitated the success of surgery to a certain extent. Short follow-up time was also a limitation of our study. More studies are needed to evaluate the results. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
28. Profiling of differentially expressed genes in sheep T lymphocytes response to an artificial primary Haemonchus contortus infection.
- Author
-
Yi Yang, Qian-Jin Zhou, Xue-Qiu Chen, Bao-Long Yan, Xiao-Lu Guo, Hong-Li Zhang, and Ai-Fang Du
- Subjects
- *
HAEMONCHUS contortus , *NEMATODES , *T cells , *HAEMONCHUS , *GROWTH factors , *DNA microarrays , *GENE expression , *METABOLISM - Abstract
Background: Haemonchus contortus is a common bloodsucking nematode causing widespread economic loss in agriculture. Upon H. contortus infection, a series of host responses is elicited, especially those related to T lymphocyte immunity. Existing studies mainly focus on the general immune responses of sheep T lymphocyte to H. contortus, lacking investigations at the molecular level. The objective of this study was to obtain a systematic transcriptional profiling of the T lymphocytes in H. contortus primary-infected sheep. Methods: Nematode-free sheep were orally infected once with H. contortus L3s. T lymphocyte samples were collected from the peripheral blood of 0, 3, 30 and 60 days post infection (dpi) infected sheep. Microarrays were used to compare gene transcription levels between samples. Quantitative RT-PCR was employed to validate the microarray data. Gene Ontology and KEGG pathway analysis were utilized for the annotation of differentially expressed genes. Results: Our microarray data was consistent with qPCR results. From microarrays, 853, 242 and 42 differentially expressed genes were obtained in the 3d vs. 0d, 30d vs. 0d and 60d vs. 0d comparison groups, respectively. Gene Ontology and KEGG pathway analysis indicated that these genes were involved in metabolism, signaling, cell growth and immune system processes. Functional analysis of significant differentially expressed genes, such as SLC9A3R2, ABCB9, COMMD4, SUGT1, FCER1G, GSK3A, PAK4 and FCER2, revealed a crucial association with cellular homeostasis maintenance and immune response. Our data suggested that maintaining both effective immunological response and natural cellular activity are important for T lymphocytes in fighting against H. contortus infection. Conclusions: Our results provide a substantial list of candidate genes in sheep T lymphocytes response to H. contortus infection, and contribute novel insights into a general immune response upon infection. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
29. Association of 42 SNPs with genetic risk for cervical cancer: an extensive meta-analysis
- Author
-
Shaoshuai Wang, Haiying Sun, Yao Jia, Fangxu Tang, Hang Zhou, Xiong Li, Jin Zhou, Kecheng Huang, Qinghua Zhang, Ting Hu, Ru Yang, Changyu Wang, Ling Xi, Dongrui Deng, Hui Wang, Shixuan Wang, Ding Ma, and Shuang Li
- Abstract
Background: A large number of single nucleotide polymorphisms (SNPs) associated with cervical cancer have been identified through candidate gene association studies and genome-wide association studies (GWAs). However, some studies have yielded different results for the same SNP. To obtain a more comprehensive understanding, we performed a meta-analysis on previously published case–control studies involving the SNPs associated with cervical cancer. Methods: Electronic searches of PubMed and Embase were conducted for all publications about the association between gene polymorphisms and cervical cancer. One-hundred and sixty-seven association studies were included in our research. For each SNP, three models (the allele, dominant and recessive effect models) were adopted in the meta-analysis. For each model, the effect summary odds ratio (OR) and 95% CI were calculated. Heterogeneity between studies was evaluated by Cochran’s Q test. If the p value of Q test was less than 0.01, a random effect model was used; otherwise, a fixed effect model was used. Results: The results of our meta-analysis showed that: (1) There were 8, 2 and 8 SNPs that were significantly associated with cervical cancer (P < 0.01) in the allele, dominant and recessive effect models, respectively. (2) rs1048943 (CYP1A1 A4889G) showed the strongest association with cervical cancer in the allele effect model (1.83[1.57, 2.13]); in addition, rs1048943 (CYP1A1 A4889G) had a very strong association in the dominant and recessive effect model. (3) 15, 11 and 10 SNPs had high heterogeneity (P < 0.01) in the three models, respectively. (4) There was no published bias for most of the SNPs according to Egger’s test (P < 0.01) and Funnel plot analysis. For some SNPs, their association with cervical cancer was only tested in a few studies and, therefore, might have been subjected to published bias. More studies on these loci are required. Conclusion: Our meta-analysis provides a comprehensive evaluation of cervical cancer association studies. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
30. Anterior cervical discectomy and fusion may be more effective than anterior cervical corpectomy and fusion for the treatment of cervical spondylotic myelopathy.
- Author
-
Li Guan, Yong Hai, Jin-Cai Yang, Li-Jin Zhou, and Xiao-Long Chen
- Subjects
DISCECTOMY ,CERVICAL spondylotic myelopathy ,META-analysis ,ODDS ratio ,BLOOD loss estimation ,THERAPEUTICS - Abstract
Background: This meta-analysis explored the efficacy and safety of anterior cervical corpectomy and fusion (ACCF) comparing to anterior cervical discectomy and fusion (ACDF) in treating cervical spondylotic myelopathy (CSM) patients. Methods: Several electronic databases were searched combined with manually searching. Thirteen randomized controlled studies were enrolled with 1,062 CSM patients, including 468 patients and 594 patients in the in the ACCF and ACDF group, respectively. The meta-analysis was then performed using the STATA 12.0 software. Crude standard mean difference (SMD) or odds ratio (OR) with their 95% confidence intervals (CI) were calculated. Results: Our meta-analysis results revealed that CSM patients in ACDF group showed less blood loss than those in ACCF group (SMD = 1.21, 95% CI = 1.03 ~ 1.39, P < 0.001). The operation time of CSM patients in the ACDF group was also obviously shorter than those in ACCF group (SMD = 0.40, 95% CI = 0.23 ~ 0.57, P < 0.001). Furthermore, CSM patients in ACDF group had shorter hospital time than those in ACCF group (SMD = 0.45, 95% CI = 0.21 ~ 0.69, P < 0.001). Conclusion: Our findings provide empirical evidence that ACDF may be more effective than ACCF for CSM treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
31. Validation and comparison of EuroQoL-5 dimension (EQ-5D) and Short Form-6 dimension (SF-6D) among stable angina patients.
- Author
-
Jing Wu, Yuerong Han, Fei-Li Zhao, Jin Zhou, Zhijun Chen, and He Sun
- Subjects
QUALITY of life ,ANGINA pectoris ,CORONARY disease ,ACUTE coronary syndrome ,MEDICAL care ,PATIENTS - Abstract
Objectives Several preference-based health-related quality of life (HRQoL) instruments have been published and widely used in different populations. However no consensus has emerged regarding the most appropriate instrument in therapeutic area of stable angina. This study compared and validated the psychometric properties of two generic preference-based instruments, the EQ-5D and SF-6D, among Chinese stable angina patients. Methods Convergent validity of the EQ-5D and SF-6D was examined with eight a priori hypotheses from stable angina patients in conjunction with Seattle Angina Questionnaire (SAQ). Responsiveness was compared using the effect size (ES), relative efficiency (RE) and receiver operating characteristic (ROC) curves. Agreement between the EQ-5D and SF-6D was tested using intra-class correlation coefficient (ICC) and Bland-Altman plot. Factors affecting utility difference were explored with multiple linear regression analysis. Results In 411 patients (mean age 68.08 ± 11.35), mean utility scores (SD) were 0.78 (0.15) for the EQ-5D and 0.68 (0.12) for the SF-6D. Validity was demonstrated by the moderate to strong correlation coefficients (Range: 0.368-0.594, P< 0.001) for five of the eight hypotheses in both the EQ-5D and SF-6D. There were no serious floor effects for the EQ-5D and SF-6D, but ceiling effects for the EQ-5D were large. The areas under ROC of them all exceeded 0.5 (0.660-0.814, P< 0.001). The SF-6D showed a better discriminative capacity (ES: 0.573 to 1.179) between groups with different stable-angina-specific health status than the EQ-5D (ES: 0.426 to 1.126). RE suggested that the SF-6D (RE: 44.8 to 177.8%) was more efficient than the EQ-5D except for physical function. Poor agreement between them was observed with ICC (0.448, P< 0.001) and Bland-Altman plot analysis. Multiple liner regression showed that clinical variables significantly (P< 0.05) influenced differences in utility scores between the EQ-5D and SF-6D. Conclusions Both EQ-5D and SF-6D are valid and sensitive preference-based HRQoL instruments in Chinese stable angina patients. The SF-6D may be a more effective tool with lower ceiling effect and greater sensitivity. Further study is needed to compare other properties, such as reliability and longitudinal response. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
32. Survey and analysis of the current state of residency training in medical-school-affiliated hospitals in China.
- Author
-
Hong Bo, Dong-Hua Zhang, Tian-Ming Zuo, Dong-Bo Xue, Jin-Song Guo, Mei-Na Liu, Jing-Zhu Dong, Bao-Zhi Sun, and Jin Zhou
- Subjects
MEDICAL schools ,CONTINUING medical education ,EDUCATIONAL resources ,POLITICAL science ,ASSOCIATIONS, institutions, etc. ,MEDICAL education - Abstract
Background Since the global standards for postgraduate medical education (PGME) were published in January 2003, they have gained worldwide attention. The current state of residency training programs in medical-school-affiliated hospitals throughout China was assessed in this study. Methods Based on the internationally recognized global standards for PGME, residents undergoing residency training at that time and the relevant residency training instructors and management personnel from 15 medical-school-affiliated hospitals throughout China were recruited and surveyed regarding the current state of residency training programs. A total of 938 questionnaire surveys were distributed between June 30, 2006 and July 30, 2006; of 892 surveys collected, 841 were valid. Results For six items, the total proportions of "basically meets standards" and "completely meets standards" were <70% for the basic standards. These items were identified in the fields of "training settings and educational resources", "evaluation of training process", and "trainees". In all fields other than "continuous updates", the average scores of the western regions were significantly lower than those of the eastern regions for both the basic and target standards. Specifically, the average scores for the basic standards on as many as 25 of the 38 items in the nine fields were significantly lower in the western regions. There were significant differences in the basic standards scores on 13 of the 38 items among trainees, instructors, and managers. Conclusions The residency training programs have achieved satisfactory outcomes in the hospitals affiliated with various medical schools in China. However, overall, the programs remain inadequate in certain areas. For the governments, organizations, and institutions responsible for PGME, such global standards for PGME are a very useful self-assessment tool and can help identify problems, promote reform, and ultimately standardize PGME. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
33. Aberrant NDRG1 methylation associated with its decreased expression and clinicopathological significance in breast cancer.
- Author
-
Lin-Lin Han, Lin Hou, Ming-Jin Zhou, Zhong-liang Ma, Dong-Liang Lin, Li Wu, and Yin-lin Ge
- Subjects
DNA methylation ,GENE expression ,CANCER cell differentiation ,GENETICS of breast cancer ,DEOXYCYTIDINE - Abstract
Background: Cancer cell differentiation is an important characteristic of malignant tumor and has a great impact on prognosis and therapeutic decision for patients. The N-myc downstream regulated gene 1 (NDRG1), a putative tumor suppression gene, is involved in the regulation of human cell differentiation and metastasis in various cancers. Changes in the status of methylation of the NDRG1 gene have not been studied in detail in human breast cancer. Results: The MDA-MB-231 breast tumor cell line could express NDRG1. However, it was only expressed after treatment with 5-Aza-2'-deoxycytidine (AZA) in T47D cell line, which revealed that NDRG1 expression could modulated by DNA methylation. Therefore, the fragment surrounding the transcript start site of NDRG1 gene promoter was cloned after sodium bisulfite DNA treatment. A high density (66%) of methylation for human NDRG1 gene promoter was detected in T47D; however, there was only 16% of methylated CpG dinucleotides in the NDRG1 gene promoter in MDA-MB-231. DNA methylation in the NDRG1 promoter was detected in 31.1% of primary breast cancer samples. Furthermore, the NDRG1 promoter methylation correlated with the Tumor Node Metastasis (TNM) at stage III/IV, metastasis, lymph invasion, moderate and poor histological grade in the breast cancer patients. Conclusion: These findings suggest that the DNA methylation status of NDRG1 gene may play an important role in the pathogenesis and/or development of breast cancer, and the expression could be regulated by aberrant DNA methylation. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
34. The spatiotemporal matching pattern of Ezrin/Periaxin involved in myoblast differentiation and fusion and Charcot-Marie-Tooth disease-associated muscle atrophy.
- Author
-
Zhang RN, Bao X, Liu Y, Wang Y, Li XY, Tan G, Mbadhi MN, Xu W, Yang Q, Yao LY, Chen L, Zhao XY, Hu CQ, Zhang JX, Zheng HT, Wu Y, Li S, Chen SJ, Chen SY, Lv J, Shi LL, and Tang JM
- Subjects
- Humans, Muscular Atrophy, Cell Differentiation, Muscle Fibers, Skeletal, Charcot-Marie-Tooth Disease, Hereditary Sensory and Motor Neuropathy
- Abstract
Background: Clinically, Charcot-Marie-Tooth disease (CMT)-associated muscle atrophy still lacks effective treatment. Deletion and mutation of L-periaxin can be involved in CMT type 4F (CMT4F) by destroying the myelin sheath form, which may be related to the inhibitory role of Ezrin in the self-association of L-periaxin. However, it is still unknown whether L-periaxin and Ezrin are independently or interactively involved in the process of muscle atrophy by affecting the function of muscle satellite cells., Method: A gastrocnemius muscle atrophy model was prepared to mimic CMT4F and its associated muscle atrophy by mechanical clamping of the peroneal nerve. Differentiating C2C12 myoblast cells were treated with adenovirus-mediated overexpression or knockdown of Ezrin. Then, overexpression of L-periaxin and NFATc1/c2 or knockdown of L-periaxin and NFATc3/c4 mediated by adenovirus vectors were used to confirm their role in Ezrin-mediated myoblast differentiation, myotube formation and gastrocnemius muscle repair in a peroneal nerve injury model. RNA-seq, real-time PCR, immunofluorescence staining and Western blot were used in the above observation., Results: For the first time, instantaneous L-periaxin expression was highest on the 6th day, while Ezrin expression peaked on the 4th day during myoblast differentiation/fusion in vitro. In vivo transduction of adenovirus vectors carrying Ezrin, but not Periaxin, into the gastrocnemius muscle in a peroneal nerve injury model increased the numbers of muscle myosin heavy chain (MyHC) I and II type myofibers, reducing muscle atrophy and fibrosis. Local muscle injection of overexpressed Ezrin combined with incubation of knockdown L-periaxin within the injured peroneal nerve or injection of knockdown L-periaxin into peroneal nerve-injured gastrocnemius muscle not only increased the number of muscle fibers but also recovered their size to a relatively normal level in vivo. Overexpression of Ezrin promoted myoblast differentiation/fusion, inducing increased MyHC-I
+ and MyHC-II + muscle fiber specialization, and the specific effects could be enhanced by the addition of adenovirus vectors for knockdown of L-periaxin by shRNA. Overexpression of L-periaxin did not alter the inhibitory effects on myoblast differentiation and fusion mediated by knockdown of Ezrin by shRNA in vitro but decreased myotube length and size. Mechanistically, overexpressing Ezrin did not alter protein kinase A gamma catalytic subunit (PKA-γ cat), protein kinase A I alpha regulatory subunit (PKA reg Iα) or PKA reg Iβ levels but increased PKA-α cat and PKA reg II α levels, leading to a decreased ratio of PKA reg I/II. The PKA inhibitor H-89 remarkably abolished the effects of overexpressing-Ezrin on increased myoblast differentiation/fusion. In contrast, knockdown of Ezrin by shRNA significantly delayed myoblast differentiation/fusion accompanied by an increased PKA reg I/II ratio, and the inhibitory effects could be eliminated by the PKA reg activator N6-Bz-cAMP. Meanwhile, overexpressing Ezrin enhanced type I muscle fiber specialization, accompanied by an increase in NFATc2/c3 levels and a decrease in NFATc1 levels. Furthermore, overexpressing NFATc2 or knocking down NFATc3 reversed the inhibitory effects of Ezrin knockdown on myoblast differentiation/fusion., Conclusions: The spatiotemporal pattern of Ezrin/Periaxin expression was involved in the control of myoblast differentiation/fusion, myotube length and size, and myofiber specialization, which was related to the activated PKA-NFAT-MEF2C signaling pathway, providing a novel L-Periaxin/Ezrin joint strategy for the treatment of muscle atrophy induced by nerve injury, especially in CMT4F., (© 2023. The Author(s).)- Published
- 2023
- Full Text
- View/download PDF
35. Insufficient tuberculosis treatment leads to earlier and higher mortality in individuals co-infected with HIV in southern China: a cohort study.
- Author
-
Zheng Z, Nehl EJ, Zhou C, Li J, Xie Z, Zhou Z, and Liang H
- Subjects
- AIDS-Related Opportunistic Infections epidemiology, AIDS-Related Opportunistic Infections virology, Adult, Aged, China epidemiology, Coinfection epidemiology, Coinfection virology, Female, Follow-Up Studies, Humans, Male, Middle Aged, Risk Factors, Survival Rate, Treatment Outcome, Tuberculosis, Pulmonary epidemiology, Tuberculosis, Pulmonary microbiology, AIDS-Related Opportunistic Infections complications, Antitubercular Agents therapeutic use, Coinfection mortality, HIV immunology, Mycobacterium tuberculosis isolation & purification, Tuberculosis, Pulmonary complications, Tuberculosis, Pulmonary drug therapy
- Abstract
Background: Tuberculosis (TB) and Acquired Immune Deficiency Syndrome (AIDS) are leading causes of death globally. However, little is known about the long-term mortality risk and the timeline of death in those co-infected with human immunodeficiency virus (HIV) and Mycobacterium tuberculosis (MTB). This study sought to understand the long-term mortality risk, factors, and the timeline of death in those with HIV-Mycobacterium tuberculosis (MTB) coinfection, particularly in those with insufficient TB treatment., Methods: TB-cause specific deaths were classified using a modified 'Coding of Cause of Death in HIV' protocol. A longitudinal cross-registration-system checking approach was used to confirm HIV/MTB co-infection between two observational cohorts. Mortality from the end of TB treatment (6 months) to post-treatment year (PTY) 5 (60 months) was investigated by different TB treatment outcomes. General linear models were used to estimate the mean mortality at each time-point and change between time-points. Cox's proportional hazard regressions measured the mortality hazard risk (HR) at each time-point. The Mantel-Haenszel stratification was used to identify mortality risk factors. Mortality density was calculated by person year of follow-up., Results: At the end point, mortality among patients with HIV/MTB coinfection was 34.7%. From the end of TB treatment to PTY5, mortality and loss of person years among individuals with TB treatment failure, missing, and adverse events (TBFMA) were significantly higher than those who had TB cure (TBC) and TB complete regimen (TBCR). Compared to individuals with TBC and with TBCR, individuals with TBFMA tended to die earlier and their mortality was significantly higher (HR
TBFMA-TBC = 3.0, 95% confidence interval: 2.5-3.6, HRTBFMA-TBCR = 2.9, 95% CI: 2.5-3.4, P < 0.0001). Those who were naïve to antiretroviral therapy, were farmers, had lower CD4 counts (≤200 cells/μL) and were ≥ 50 years of age were at the highest risk of mortality. Mortality risk for participants with TBFMA was significantly higher across all stratifications except those with a CD4 count of ≤200 cells/μL., Conclusions: Earlier and long-term mortality among those with HIV/MTB co-infection is a significant problem when TB treatment fails or is inadequate.- Published
- 2020
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.