1. Identification of a cytokine network sustaining neutrophil and Th17 activation in untreated early rheumatoid arthritis
- Author
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M. Margarida Souto-Carneiro, Rita Cascão, Helena Canhão, Joaquim Polido-Pereira, Luis Graca, Rita A Moura, Ana M. Rodrigues, João Eurico Fonseca, I.P. Perpétuo, H S Rosário, M Viana Queiroz, Ana Filipa Mourão, E Vieira de Sousa, and Repositório da Universidade de Lisboa
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Male ,Neutrophils ,medicine.medical_treatment ,lcsh:Medicine ,Arthritis ,Disease ,Lymphocyte Activation ,Arthritis, Rheumatoid ,0302 clinical medicine ,Synovial Fluid ,Immunology and Allergy ,0303 health sciences ,Neutrophil ,General Medicine ,Middle Aged ,Flow Cytometry ,3. Good health ,Cytokine ,Cytokine Network ,030220 oncology & carcinogenesis ,Rheumatoid arthritis ,Cytokines ,Female ,Th17 ,medicine.symptom ,Research Article ,medicine.medical_specialty ,Immunology ,Inflammation ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Rheumatology ,Synovitis ,Internal medicine ,medicine ,Synovial fluid ,Humans ,030304 developmental biology ,030203 arthritis & rheumatology ,Autoimmune disease ,business.industry ,lcsh:R ,Early rheumatoid arthritis ,medicine.disease ,Poster Presentation ,Th17 Cells ,business - Abstract
© 2010 Cascão et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited., Introduction: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by sustained synovitis. Recently, several studies have proposed neutrophils and Th17 cells as key players in the onset and perpetuation of this disease. The main goal of this work was to determine whether cytokines driving neutrophil and Th17 activation are dysregulated in very early rheumatoid arthritis patients with less than 6 weeks of disease duration and before treatment (VERA). Methods: Cytokines related to neutrophil and Th17 activation were quantified in the serum of VERA and established RA patients and compared with other very early arthritis (VEA) and healthy controls. Synovial fluid (SF) from RA and osteoarthritis (OA) patients was also analyzed. Results: VERA patients had increased serum levels of cytokines promoting Th17 polarization (IL-1b and IL-6), as well as IL-8 and Th17-derived cytokines (IL-17A and IL-22) known to induce neutrophil-mediated inflammation. In established RA this pattern is more evident within the SF. Early treatment with methotrexate or corticosteroids led to clinical improvement but without an impact on the cytokine pattern. Conclusions: VERA patients already display increased levels of cytokines related with Th17 polarization and neutrophil recruitment and activation, a dysregulation also found in SF of established RA. 0 Thus, our data suggest that a cytokine-milieu favoring Th17 and neutrophil activity is an early event in RA pathogenesis., This work was supported by a grant from Sociedade Portuguesa de Reumatologia/Schering-Plough 2005. RAM and RC were funded by Fundação para a Ciência e a Tecnologia (FCT) SFRH/BD/30247/2006 and SFRH/BD/40513/2007, respectively. MMS-C was funded by Marie Curie Intra-European Fellowship PERG-2008-239422 and a EULAR Young Investigator Award.
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- 2010