1. Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression
- Author
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Xuewen Xu, Michel Georges, Tracy Hadfield, Noelle E. Cockett, Huijun Cheng, Carole Charlier, and Haruko Takeda
- Subjects
Reporter assay ,Locus (genetics) ,Trans-effect ,Biology ,Transfection ,Genomic Imprinting ,microRNA ,Chlorocebus aethiops ,Genetics ,Animals ,Muscle development ,Polar overdominance ,Muscle, Skeletal ,Regulation of gene expression ,Reporter gene ,Sheep ,DLK1 ,MicroRNA ,MicroRNAs ,Gene Expression Regulation ,COS Cells ,Intercellular Signaling Peptides and Proteins ,DNA microarray ,Genomic imprinting ,Callipyge ,Post-transcriptional gene regulation ,Biotechnology ,Research Article - Abstract
Background Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect. Results We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo. Conclusions This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect. Electronic supplementary material The online version of this article (doi:10.1186/1471-2164-15-944) contains supplementary material, which is available to authorized users.
- Published
- 2014