Search

Your search keyword '"Hughes, Jacqueline"' showing total 6 results
Start Over Author "Hughes, Jacqueline" Publisher biomed central
6 results on '"Hughes, Jacqueline"'

2. Health behaviour change among UK adults during the pandemic: findings from the COVID-19 cancer attitudes and behaviours study

Author

Anyanwu, Philip, Moriarty, Yvonne, McCutchan, Grace, Grozeva, Detelina, Goddard, Mark, Whitelock, Victoria, Cannings-John, Rebecca, Quinn-Scoggins, Harriet, Hughes, Jacqueline, Gjini, Ardiana, Hepburn, Julie, Osborne, Kirstie, Robling, Michael, Townson, Julia, Waller, Jo, Whitaker, Katriina L., Brown, Jamie, Brain, Kate, and Moore, Graham

Published
2022
Full Text
View/download PDF

3. Development, implementation and evaluation of an evidence-based paediatric early warning system improvement programme: the PUMA mixed methods study

Author

Allen, Davina, Lloyd, Amy, Edwards, Dawn, Hood, Kerenza, Huang, Chao, Hughes, Jacqueline, Jacob, Nina, Lacy, David, Moriarty, Yvonne, Oliver, Alison, Preston, Jennifer, Sefton, Gerri, Sinha, Ian, Skone, Richard, Strange, Heather, Taiyari, Khadijeh, Thomas-Jones, Emma, Trubey, Rob, Tume, Lyvonne, Powell, Colin, and Roland, Damian

Published
2022
Full Text
View/download PDF

5. A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper

Author

Hughes Jacqueline M, Hide Geoff, and McNuff Robert

Subjects
Ecology, Tubulin, Methodology Article, Animals, Water, Ciliophora, DNA, Protozoan, Polymerase Chain Reaction, Sensitivity and Specificity, QH540-549.5, DNA Primers
Abstract

Background The rapid expansion in the availability of genome and DNA sequence information has opened up new possibilities for the development of methods for detecting free-living protozoa in environmental samples. The protozoan Blepharisma japonicum was used to investigate a rapid and simple detection system based on polymerase chain reaction amplification (PCR) from organisms immobilised on FTA paper. Results Using primers designed from the α-tubulin genes of Blepharisma, specific and sensitive detection to the equivalent of a single Blepharisma cell could be achieved. Similar detection levels were found using water samples, containing Blepharisma, which were dried onto Whatman FTA paper. Conclusion This system has potential as a sensitive convenient detection system for Blepharisma and could be applied to other protozoan organisms.

Published
2003

6. A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper.

Author

Hide, Geoff, Hughes, Jacqueline M., and McNuff, Robert

Subjects
BLEPHARISMA, POLYMERASE chain reaction, GENOMES, NUCLEOTIDE sequence, TUBULINS, PROTOZOA
Abstract

Background: The rapid expansion in the availability of genome and DNA sequence information has opened up new possibilities for the development of methods for detecting free-living protozoa in environmental samples. The protozoan Blepharisma japonicum was used to investigate a rapid and simple detection system based on polymerase chain reaction amplification (PCR) from organisms immobilised on FTA paper. Results: Using primers designed from the a-tubulin genes of Blepharisma, specific and sensitive detection to the equivalent of a single Blepharisma cell could be achieved. Similar detection levels were found using water samples, containing Blepharisma, which were dried onto Whatman FTA paper. Conclusion: This system has potential as a sensitive convenient detection system for Blepharisma and could be applied to other protozoan organisms. [ABSTRACT FROM AUTHOR]

Published
2003

Catalog

Books, media, physical & digital resources
See catalog results