27 results on '"Hao Liang"'
Search Results
2. Deubiquitinase USP10 promotes osteosarcoma autophagy and progression through regulating GSK3β-ULK1 axis
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Feng, Zuxi, Ou, Yanghuan, Deng, Xueqiang, Deng, Minghao, Yan, Xiaohua, Chen, Leifeng, Zhou, Fan, and Hao, Liang
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- 2024
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3. Anterior knee pain as a potential risk factor for falls in older adults: insights from the osteoarthritis initiative data
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Xiong, Ting, Ou, Yanghuan, Chen, Shenliang, Liu, Shuaigang, Yi, Xuan, Deng, Xueqiang, Cheng, Tao, and Hao, Liang
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- 2023
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4. RBX1 regulates PKM alternative splicing to facilitate anaplastic thyroid carcinoma metastasis and aerobic glycolysis by destroying the SMAR1/HDAC6 complex
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Xu, Debin, Yu, Jichun, Yang, Yuting, Du, Yunyan, Lu, Hongcheng, Zhang, Shouhua, Feng, Qian, Yu, Yi, Hao, Liang, Shao, Jun, and Chen, Leifeng
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- 2023
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5. Clinical significance of FBXW7 tumor suppressor gene mutations and expression in human colorectal cancer: a systemic review and meta-analysis
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Shang, Wei, Yan, Chuanwang, Liu, Ran, Chen, Lili, Cheng, Dongdong, Hao, Liang, Yuan, Wenguang, Chen, Jingbo, and Yang, Hui
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- 2021
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6. Developing a reliable and valid competency model for assistant dentists in China
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Hao Liang, Zhong Chen, Yueping Li, Qinyue Dai, Xiaomei Hong, and Yingzhen Lai
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China ,Oral health ,Dentists ,Delphi method ,Developing country ,Structural equation modeling ,Education ,Surveys and Questionnaires ,Humans ,Competence (human resources) ,Reliability (statistics) ,computer.programming_language ,Health services research, assistant dentists ,Medical education ,Characteristics of oral professionals ,LC8-6691 ,Dental Assistant ,Research ,Reproducibility of Results ,General Medicine ,Focus group ,Special aspects of education ,Medicine ,Competency model ,Psychology ,Factor Analysis, Statistical ,computer ,Delphi - Abstract
Background The shortage of dentists is one of the various medical-resource problems found around the world. More and more countries are improving the provision of oral services by training dental therapists and hygienists. In China, they are called assistant dentists, and they supplement dental services, but thus far, no research has been conducted on the competence of this group. Objective The purpose of this study was to establish a competency model for Chinese assistant dentists. This model can provide a more scientific basis for the education, training, and evaluation of these professionals, as well as provide a reference for the capacity of dental therapists in various countries. Methods We established a system of competency characteristics using theoretical analysis and focus group discussions, after which we established an initial competency model by consulting a Delphi panel of 29 experts. Finally, we collected data from 1389 assistant dentists from 14 provinces in China, and the reliability and validity of the model were confirmed by factor analysis of this data. Results After three rounds of Delphi panels, the competency model came to include seven ability levels and 50 indicators. In exploratory-factor analysis, three indicators were eliminated, and the questionnaire could explain 68.41 % of total variance. In confirmatory-factor analysis, the established model and data fit well (goodness-of-fit index [GFI] = 0.914, root mean square error of approximation [RMSEA] = 0.047). The results showed that the entire model has good reliability and validity. Conclusions Our competency model for dental assistants in China includes seven elements. This is consistent with the current health situation in China, and it has distinct Chinese characteristics. Some of our findings, like those reported in other countries with dental therapists, offer ideas for other developing countries.
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- 2021
7. A case of paraplegia due to asymptomatic varicella-zoster virus infection in AIDS patient unexpectedly diagnosed by CSF metagenomic next-generation sequencing
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Zhiman Xie, Guangjing Ruan, Chuanyi Ning, Jingzhen Lai, and Hao Liang
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Adult ,Male ,medicine.medical_specialty ,Pediatrics ,Herpesvirus 3, Human ,viruses ,Myelitis ,Case Report ,Disease ,Infectious and parasitic diseases ,RC109-216 ,medicine.disease_cause ,Asymptomatic ,Herpes Zoster ,Virus ,Medical microbiology ,Acquired immunodeficiency syndrome (AIDS) ,medicine ,Humans ,Paraplegia ,Acquired Immunodeficiency Syndrome ,integumentary system ,business.industry ,Varicella zoster virus ,virus diseases ,HIV ,High-Throughput Nucleotide Sequencing ,medicine.disease ,AIDS ,Infectious Diseases ,mNGS ,Varicella Zoster Virus Infection ,Varicella-zoster virus ,medicine.symptom ,business - Abstract
Background Varicella-zoster virus (VZV) infection may induce central nervous system complications in HIV/AIDS patients. However, it is rare to have paraplegia caused by VZV infection but no herpes zoster clinically. Asymptomatic VZV infection in HIV/AIDS patient increased the difficulty of diagnosis. Case presentation We reported a 41-year-old male AIDS patient with rare asymptomatic VZV infection-induced paraplegia after his anti-retroviral therapy initiation. MRI of the spinal cord showed the morphology of the thoracic spinal cord was irregular and locally inflated. The patient was confirmed as VZV induced thoracic myelomyelitis by using the cerebrospinal fluid for metagenomic next-generation sequencing (mNGS). Conclusions mNGS may contribute to disease diagnosis for asymptomatic VZV infection-induced myelitis.
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- 2021
8. Insufficient tuberculosis treatment leads to earlier and higher mortality in individuals co-infected with HIV in southern China: a cohort study
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Zhigang Zheng, Zhouhua Xie, Zijun Zhou, Chongxing Zhou, Eric J. Nehl, Hao Liang, and Jianjun Li
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Adult ,Male ,medicine.medical_specialty ,China ,Tuberculosis ,Antitubercular Agents ,010502 geochemistry & geophysics ,01 natural sciences ,03 medical and health sciences ,0302 clinical medicine ,Acquired immunodeficiency syndrome (AIDS) ,Risk Factors ,Internal medicine ,medicine ,Risk of mortality ,Humans ,030212 general & internal medicine ,Adverse effect ,Tuberculosis, Pulmonary ,0105 earth and related environmental sciences ,Cause of death ,Aged ,AIDS-Related Opportunistic Infections ,business.industry ,Coinfection ,HIV ,Mycobacterium tuberculosis ,Middle Aged ,medicine.disease ,Survival Rate ,Regimen ,Infectious Diseases ,Treatment Outcome ,Female ,business ,Cohort study ,Research Article ,Follow-Up Studies - Abstract
BackgroundTuberculosis (TB) and Acquired Immune Deficiency Syndrome (AIDS) are leading causes of death globally. However, little is known about the long-term mortality risk and the timeline of death in those co-infected with human immunodeficiency virus (HIV) andMycobacterium tuberculosis(MTB). This study sought to understand the long-term mortality risk, factors, and the timeline of death in those with HIV-Mycobacterium tuberculosis(MTB) coinfection, particularly in those with insufficient TB treatment.MethodsTB-cause specific deaths were classified using a modified ‘Coding of Cause of Death in HIV’ protocol. A longitudinal cross-registration-system checking approach was used to confirm HIV/MTB co-infection between two observational cohorts. Mortality from the end of TB treatment (6 months) to post-treatment year (PTY) 5 (60 months) was investigated by different TB treatment outcomes. General linear models were used to estimate the mean mortality at each time-point and change between time-points. Cox’s proportional hazard regressions measured the mortality hazard risk (HR) at each time-point. The Mantel-Haenszel stratification was used to identify mortality risk factors. Mortality density was calculated by person year of follow-up.ResultsAt the end point, mortality among patients with HIV/MTB coinfection was 34.7%. From the end of TB treatment to PTY5, mortality and loss of person years among individuals with TB treatment failure, missing, and adverse events (TBFMA) were significantly higher than those who had TB cure (TBC) and TB complete regimen (TBCR). Compared to individuals with TBC and with TBCR, individuals with TBFMA tended to die earlier and their mortality was significantly higher (HRTBFMA-TBC = 3.0, 95% confidence interval: 2.5–3.6, HRTBFMA-TBCR = 2.9, 95% CI: 2.5–3.4,P ConclusionsEarlier and long-term mortality among those with HIV/MTB co-infection is a significant problem when TB treatment fails or is inadequate.
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- 2020
9. Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis
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Dongjun Liu, Yanhua Dong, Ju Zhang, Datong Lu, Hao Liang, Nan Yang, Jianfei Ma, Jie Yang, and Ming Cang
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0301 basic medicine ,White fat cell proliferation ,Endocrinology, Diabetes and Metabolism ,Adipose Tissue, White ,Clinical Biochemistry ,Proliferation ,Brown fat ,Adipocytes, White ,Blotting, Western ,Adipose tissue ,Apoptosis ,White adipose tissue ,Biology ,Dlgap1 ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Endocrinology ,Adipose Tissue, Brown ,Gene expression ,Browning ,Animals ,lcsh:RC620-627 ,Cell Proliferation ,Cell growth ,Research ,Biochemistry (medical) ,Cell biology ,SAP90-PSD95 Associated Proteins ,White (mutation) ,lcsh:Nutritional diseases. Deficiency diseases ,030104 developmental biology ,Adipocytes, Brown ,White fat ,030217 neurology & neurosurgery - Abstract
Background Obesity is a metabolic imbalance characterized by excessive deposition of white fat. The browning of white fat can effectively treat obesity and related diseases. Although Dlgap1 (Discs, Large (Drosophila) Homolog-Associated Protein 1) is suspected to have an effect on this process, no empirical evidence is available. Methods To understand the role of Dlgap1, we cultured white and brown fat cells, then performed overexpression and knockout experiments. Results We found that Dlgap1 overexpression in brown adipocytes inhibits brown-fat-related gene expression, promotes white-fat-related genes, while also increasing brown-adipocyte proliferation and apoptosis. However, the gene overexpression has no effect on brown adipocyte maturation. Knocking out Dlgap1 in white fat cells promotes the expression and inhibition of brown-fat-related and white-fat-related genes, respectively. Additionally, the knockout inhibits white fat cell proliferation and apoptosis, while also promoting their maturation. Conclusions Dlgap1 negatively regulates the browning of white adipocytes by influencing cell proliferation and apoptosis.
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- 2020
10. HIV prevalence among female sex workers in Guigang City, Guangxi, China: an 8-year consecutive cross-sectional study.
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Jingzhen Lai, Chunwei Qin, Nehl, Eric J., Junjun Jiang, Yunxuan Huang, Bingyu Liang, Yuexiang Xu, Jiegang Huang, Zhiliang Xu, Chuanyi Ning, Yanyan Liao, Ning Zang, Wudi Wei, Fengxiang Qin, Jun Yu, Li Ye, Xionglin Qin, Hao Liang, Lai, Jingzhen, and Qin, Chunwei
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Background: Female sex workers (FSW) are a population that are at high risk for HIV infection, and their HIV/AIDS knowledge levels and sexual behaviors are of concern. This study describes changes in HIV prevalence and factors associated among female sex workers in Guigang City, Guangxi, one of the highest HIV prevalence areas in China.Methods: Data were derived from an annual cross-sectional venue-based survey, 2008 to 2015, in the form of sentinel surveillance. The participants were recruited using cluster sampling. FSW aged 16 years and above who completed a questionnaire and HIV testing. Both descriptive and multi-level analyses were used to explore factors associated with changes in HIV prevalence.Results: Seven thousand four hundred ninety-six FSW were recruited in this study. HIV prevalence among FSW in Guigang City fell into two periods, one with an increasing trend (2008-2011) and one with a decline (2012-2015). Differences between these time periods included age, relationship status, HIV knowledge, consistent condom use, lifetime illicit drug use, history of sexually transmitted infection in the past year, HIV testing, receipt of a condom distribution and education program or HIV counseling and testing, and peer education services.Conclusions: Since 2012, a reduction in HIV prevalence among FSW in Guigang City has been observed. The decline of HIV prevalence was associated with coinciding changes in demographic characteristics of FSW, improvement of HIV knowledge and safer sexual behaviors, and a program that promotes condom use, HIV counseling & testing, and peer education. [ABSTRACT FROM AUTHOR]- Published
- 2018
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11. Platelet counts modulate the quantitative relationship between hepatitis B viral DNA and surface antigen concentrations: a cross-sectional study of hematological, histological and viral factors.
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Chao-Wei Hsu, Kung-Hao Liang, Chih-Lang Lin, Tong-Hong Wang, Chau-Ting Yeh, Hsu, Chao-Wei, Liang, Kung-Hao, Lin, Chih-Lang, Wang, Tong-Hong, and Yeh, Chau-Ting
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PLATELET count , *HEPATITIS B virus , *HEMATOLOGY , *LIVER biopsy , *MULTIVARIATE analysis , *DNA analysis , *BIOPSY , *DNA , *HEPATITIS viruses , *CIRRHOSIS of the liver , *RESEARCH funding , *SERODIAGNOSIS , *VIRAL antigens , *VIRAL load , *CROSS-sectional method , *DISEASE progression , *CHRONIC hepatitis B - Abstract
Background: The concentrations of hepatitis B virus (HBV) DNA and surface antigen (HBsAg) are two critical virological variables to be monitored in chronic hepatitis B. HBsAg is derived from the HBV genome. Thus, higher HBV-DNA concentrations should implicate higher HBsAg levels. Nevertheless, the two variables do not manifest a simple linear relationship due to elusive host factor involvements. The aim of this study was to address the discrepancy of HBV DNA and HBsAg levels by a quantitative modeling of HBsAg concentrations.Methods: Pretreatment hematological, histological and virus serological records of 327 chronic hepatitis B patients were reviewed. Two independent patient cohorts were used for validation.Results: Univariate/multivariate analysis showed that ISHAK fibrosis stages, HBV-DNA levels and hepatitis e-antigen status were independently associated with HBsAg concentrations. In agreement with the natural history of chronic hepatitis B, HBsAg concentrations were negatively correlated with ISHAK fibrosis stages (adjusted P = 0.002). Subgroup analysis showed that significant HBsAg-DNA correlation existed in high-viral-titer patients with HBV-DNA > 6 log10 IU/mL (P < 0.001), but not in low-viral-titer patients with HBV-DNA ≤ 6 log10 IU/mL (P = 0.076). A backward stepwise linear regression analysis in the low-viral-titer subgroup revealed a significant correlation between HBsAg levels and a linear combination of HBV-DNA levels and platelet counts. A biphasic model was thus established to accommodate patients with high and low HBV-DNA titers:[Formula: see text] The estimated HBsAg concentrations correlated well with the measured HBsAg levels not only in the model construction cohort (N =327, P < 0.001), but also in two validation cohorts comprising respectively the patients who had received pretreatment liver biopsy assessments (N = 45, P = 0.001), and the treatment-naïve patients who had not received liver biopsy (N = 80, P < 0.001).Conclusion: HBsAg concentrations can be quantitatively estimated by viral DNA concentrations and human platelet counts. [ABSTRACT FROM AUTHOR]- Published
- 2017
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12. Long term follow up and retrospective study on 533 gastric cancer cases
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Chun-Wei Peng, Shu-Guang Yan, Hao-Liang Zhao, Lin-Wei Wang, Jian-Ding Li, Wei-Juan Zeng, Yan Li, Guifang Yang, and Wen-Qin Hu
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Adult ,Male ,medicine.medical_specialty ,Palliative care ,Adenocarcinoma ,GC-specific overall survival ,Gastrectomy ,Risk Factors ,Stomach Neoplasms ,medicine ,Humans ,Stage (cooking) ,Survival analysis ,Aged ,Retrospective Studies ,Aged, 80 and over ,Univariate analysis ,business.industry ,Proportional hazards model ,Palliative Care ,Cancer ,Retrospective cohort study ,General Medicine ,Middle Aged ,medicine.disease ,Prognosis ,Survival Analysis ,Surgery ,Treatment Outcome ,Multivariate analysis ,Female ,business ,Gastric cancer ,Clinical pathological factors ,Research Article ,Follow-Up Studies - Abstract
Background Gastric cancer (GC) is the third leading cause of cancer death in China and the outcome of GC patients is poor. The aim of the research is to study the prognostic factors of gastric cancer patients who had curative intent or palliative resection, completed clinical database and follow-up. Methods This retrospective study analyzed 533 GC patients from three tertiary referral teaching hospitals from January 2004 to December 2010 who had curative intent or palliative resection, complete clinical database and follow-up information. The GC-specific overall survival (OS) status was determined by the Kaplan-Meier method, and univariate analysis was conducted to identify possible factors for survival. Multivariate analysis using the Cox proportional hazard model and a forward regression procedure was conducted to define independent prognostic factors. Results By the last follow-up, the median follow-up time of 533 GC patients was 38.6 mo (range 6.9-100.9 mo), and the median GC-specific OS was 25.3 mo (95% CI: 23.1-27.4 mo). The estimated 1-, 2-, 3- and 5-year GC-specific OS rates were 78.4%, 61.4%, 53.3% and 48.4%, respectively. Univariate analysis identified the following prognostic factors: hospital, age, gender, cancer site, surgery type, resection type, other organ resection, HIPEC, LN status, tumor invasion, distant metastases, TNM stage, postoperative SAE, systemic chemotherapy and IP chemotherapy. In multivariate analysis, seven factors were identified as independent prognostic factors for long term survival, including resection type, HIPEC, LN status, tumor invasion, distant metastases, postoperative SAE and systemic chemotherapy. Conclusions Resection type, HIPEC, postoperative SAE and systemic chemotherapy are four independent prognostic factors that could be intervened for GC patients for improving survival.
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- 2014
13. Dolutegravir(DTG, S/GSK1349572) combined with other ARTs is superior to RAL- or EFV-based regimens for treatment of HIV-1 infection: a meta-analysis of randomized controlled trials.
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Junjun Jiang, Xi Xu, Wenqin Guo, Jinming Su, Jiegang Huang, Bingyu Liang, Hui Chen, Ning Zang, Yanyan Liao, Li Ye, and Hao Liang
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COMBINATION drug therapy ,CONFIDENCE intervals ,HIV infections ,MEDICAL information storage & retrieval systems ,MEDLINE ,META-analysis ,ONLINE information services ,PATIENT safety ,SYSTEMATIC reviews ,RELATIVE medical risk ,TREATMENT effectiveness ,DATA analysis software ,HIV integrase inhibitors ,DESCRIPTIVE statistics ,PHARMACODYNAMICS - Abstract
Background: The first-generation integrase inhibitors (INIs) raltegravir (RAL) and elvitegravir (EVG) have shown efficacy against HIV infection, but they have the limitations of once-more daily dosing and extensive cross-resistance. Dolutegravir (DTG, S/GSK1349572), a second-generation drug that overcomes such shortcomings, is under spotlight. The purpose of this study is to review the evidence for DTG use in clinical settings, including its efficacy and safety. Methods: PubMed, EMbase, Ovid, Web of Science, Science Direct, and related websites were screened from establishment until July 2013, and scientific meeting proceedings were manually searched. Two reviewers independently screened 118 citations repeatedly to identify randomized controlled trials comparing the efficacy and safety of DTG-based regimen with those of RAL- or elvitegravir-based regimens. Using the selected studies with comparable outcome measures and indications, we performed a meta-analysis based on modified intention-to-treat (mITT), on-treatment (OT), and as-treated (AT) virological outcome data. Independent data extraction and quality assessment were conducted. Results: Four unique studies were included with the use of DTG in antiretroviral therapy-naive patients. In therapy-naive patients, DTG combined with abacavir/lamivudine (ABC/3TC) or tenofovir/emtricitabine (TDF/FTC) resulted in a signiicantly better virological outcome with a mITT relative risk (RR)of 1.07 (95 % conidence interval (95 % CI 1.03-1.12). Evidence further supported use of DTG had a better virological suppression in the 50 mg once daily group (mITT RR 1.07; 95 % CI 1.03-1.12) as well as in the sub-analysis in dolutegravir/efavirenz(DTG/EFV) and dolutegravir/ raltegravir (DTG/RAL) groups (RR 1.09, 95 % CI 1.03-1.15; RR 1.06, 95 % CI 0.98-1.15, respectively). In the matter of safety of DTG-based regimen, the risk of any event was RR 0.98 (95 % CI 0.94-1.01), the risk of serious adverse events (AEs) was RR 0.84 (95 % CI 0.62-1.15), and the risk of drug-related serious AEs was RR 0.33 (95 % CI 0.13-0.79). Conclusion: In general, DTG 50 mg given once daily combined with an active background drug is a better choice in terms of both efficacy and safety. [ABSTRACT FROM AUTHOR]
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- 2016
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14. Relationship between fasting glucose levels and in-hospital mortality in Chinese patients with acute myocardial infarction and diabetes mellitus: a retrospective cohort study.
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Hao Liang, Yi Chen Guo, Li Ming Chen, Min Li, Wei Zhong Han, Xu Zhang, Shi Liang Jiang, Liang, Hao, Guo, Yi Chen, Chen, Li Ming, Li, Min, Han, Wei Zhong, Zhang, Xu, and Jiang, Shi Liang
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GLUCOSE in the body ,MYOCARDIAL infarction-related mortality ,MYOCARDIAL infarction ,DIABETES ,MORTALITY ,HEART function tests ,PATIENTS ,DIAGNOSIS of diabetes ,TREATMENT of diabetes ,MYOCARDIAL infarction diagnosis ,MYOCARDIAL infarction treatment ,BLOOD sugar ,CHI-squared test ,FASTING ,HOSPITAL care ,MULTIVARIATE analysis ,RISK assessment ,TIME ,LOGISTIC regression analysis ,DISEASE incidence ,DISEASE prevalence ,RETROSPECTIVE studies ,HOSPITAL mortality ,ODDS ratio - Abstract
Background: Previous studies have demonstrated that elevated admission and fasting glucose (FG) is associated with worse outcomes in patients with acute myocardial infarction (AMI). However, the quantitative relationship between FG levels and in-hospital mortality in patients with AMI remains unknown. The aim of the study is to assess the prevalence of elevated FG levels in hospitalized Chinese patients with AMI and diabetes mellitus and to determine the quantitative relationship between FG levels and the in-hospital mortality as well as the optimal level of FG in patients with AMI and diabetes mellitus.Methods: A retrospective study was carried out in 1856 consecutive patients admitted for AMI and diabetes mellitus from 2002 to 2013. Clinical variables of baseline characteristics, in-hospital management and in-hospital adverse outcomes were recorded and compared among patients with different FG levels.Results: Among all patients recruited, 993 patients (53.5 %) were found to have FG ≥100 mg/dL who exhibited a higher in-hospital mortality than those with FG < 100 mg/dL (P < 0.001). Although there was a high correlation between FG levels and in-hospital mortality in all patients (r = 0.830, P < 0.001), the relationship showed a J-curve configuration with an elevated mortality when FG was less than 80 mg/dL. Using multivariate logistic regression models, we identified that age, FG levels and Killip class of cardiac function were independent predictors of in-hospital mortality in AMI patients with diabetes mellitus.Conclusions: More than half of patients with AMI and diabetes mellitus have FG ≥100 mg/dL and the relationship between in-hospital mortality and FG level was a J-curve configuration. Both FG ≥ 100 mg/dL and FG <80 mg/dL were identified to be independent predictors of in-hospital mortality and thus the optimal FG level in AMI patients with diabetes mellitus appears to be 80-100 mg/dL. [ABSTRACT FROM AUTHOR]- Published
- 2016
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15. A gene expression restriction network mediated by sense and antisense Alu sequences located on protein-coding messenger RNAs
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Chau-Ting Yeh and Kung-Hao Liang
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Genetics ,Double-stranded Alu ,RNA ,Alu element ,Retrotransposon ,Biology ,Alu-carrying protein-coding RNA ,DNA, Antisense ,Gene expression profiling ,HEK293 Cells ,Alu Elements ,Sense (molecular biology) ,Gene expression restriction ,Humans ,Nucleic Acid Conformation ,Human genome ,Gene Regulatory Networks ,RNA, Messenger ,DNA microarray ,Antisense Alu ,Gene ,Biotechnology ,Research Article - Abstract
Background Alus are primate-specific retrotransposons which account for 10.6% of the human genome. A large number of protein-coding mRNAs are encoded with sense or antisense Alus in the un-translated regions. Results We postulated that mRNAs carrying Alus in the two opposite directions can generate double stranded RNAs, capable of regulating the levels of other Alu-carrying mRNAs post-transcriptionally. A gene expression profiling assay showed that the levels of antisense and sense Alus-carrying mRNAs were suppressed in a reversible manner by over-expression of exogenous sense and antisense Alus derived from mRNAs (Family-wise error rate P= 0.0483 and P < 0.0001 respectively). Screening through human mRNAs on the NCBI-RefSeq database, it was found that sense and antisense Alu-carrying transcripts were enriched in distinct cellular functions. Antisense Alu-carrying genes were particularly enriched in neurological and developmental processes, while sense Alu-carrying genes were enriched in immunological functions. Conclusions Taken together, we proposed a novel Alu-mediated regulation network capable of stabilizing Alu-carrying mRNA levels in different cell types and restricting the activated expression levels of protein-coding, Alu-carrying mRNAs.
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- 2013
16. Development of a non-invasive fibrosis test for chronic hepatitis B patients and comparison with other unpatented scores
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Shiu-Feng Huang, Kung-Hao Liang, Kuo-Chien Tsao, Chao-Wei Hsu, and Chau-Ting Yeh
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Adult ,Liver Cirrhosis ,Male ,medicine.medical_specialty ,Cirrhosis ,General Biochemistry, Genetics and Molecular Biology ,Receiver operation characteristic curve ,Cohort Studies ,Hepatitis B, Chronic ,Liver Function Tests ,Fibrosis ,Internal medicine ,medicine ,Humans ,Medicine(all) ,Prothrombin time ,medicine.diagnostic_test ,Biochemistry, Genetics and Molecular Biology(all) ,business.industry ,Score ,Retrospective cohort study ,General Medicine ,Hepatitis B ,Middle Aged ,medicine.disease ,Surgery ,Cohort ,Female ,business ,Liver function tests ,Cohort study ,Research Article - Abstract
Background Despite the availability of patented non-invasive methods, evaluation of the degrees of liver fibrosis remains difficult when conducting a retrospective study. Such inadequacy is largely caused by requirement of biochemical parameters rarely performed in routine clinical tests. We developed a novel fibrosis HB-F score using commonly performed tests for HBV infected patients. Methods 424 patients with chronic HBV infection were included. Using clinical and virological data, HB-F score was developed from a training cohort (n = 213) and validated in a separate cohort (n = 211). The performance was compared with five other unpatented scores using ROC curves. Results Univariate and multivariate analysis revealed that age, AST/ALT ratio, platelet count and prothrombin time prolongation were significantly associated with the ISHAK fibrosis score, and were used to calculate the HB-F score. When HB-F was used to assess prominent fibrosis and cirrhosis, the AUC was 0.81 and 0.80 respectively in the training cohort, and 0.80 and 0.76 respectively in the validation cohort. HB-F had the highest AUC compared with other scores. Furthermore, in assessing paired liver biopsies with increase or decrease of ISHAK scores, HB-F showed significant change in the same direction. Conclusions A new non-invasive score was developed, which could be used to assess severity of liver fibrosis in retrospective longitudinal studies in HBV patients.
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- 2013
17. The distribution of jhp0940, jhp0945, jhp0947, jhp0949 and jhp0951 genes of Helicobacter pylori in China.
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Yanan Gong, Xianhui Peng, Lihua He, Hao Liang, Yuanhai You, Jianzhong Zhang, Gong, Yanan, Peng, Xianhui, He, Lihua, Liang, Hao, You, Yuanhai, and Zhang, Jianzhong
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HELICOBACTER pylori ,BACTERIAL genes ,PHYSIOLOGICAL adaptation ,GASTRITIS ,PEPTIC ulcer ,POLYMERASE chain reaction ,PATIENTS - Abstract
Background: The plasticity region of Helicobacter pylori (H. pylori) is a large chromosomal segment containing strain-specific genes. The prevalence of the plasticity region genes of the H. pylori strains in China remains unknown. The aim of this study was to examine the status of these genes and to assess the relationship between the genes and the diseases caused by H. pylori infection.Methods: A total of 141 strains were isolated from patients with chronic active gastritis (CAG), peptic ulcer disease (PUD) and gastric carcinoma (GC). The prevalence of jhp0940, jhp0945, jhp0947, jhp0949 and jhp0951 was determined using PCR, and the results were analyzed using the chi-squared test.Results: The prevalence rates of jhp0940, jhp0945, jhp0947, jhp0949 and jhp0951 in the H. pylori strains were 42.55, 51.06, 20.57, 56.03 and 63.12 %, respectively. The prevalence rates of jhp0940 were similar in the isolates from the CAG, PUD and GC patients, and there was no association between the jhp0940 status and any of the diseases. In contrast, the prevalence rates of jhp0945, jhp0947, jhp0949 and jhp0951 were significantly higher in the PUD and GC isolates than in the CAG isolates (p < 0.01). A univariate analysis showed that jhp0945, jhp0947, jhp0949 and jhp0951 increased the risk of PUD, while only jhp0951 was significantly associated with PUD in the multivariate analysis (p = 0.0149). The jhp0945-positive isolates were significantly associated with an increased risk for GC (p = 0.0097).Conclusion: The plasticity region genes are widely distributed in Chinese patients, and a high prevalence of these genes occurs in more serious diseases. Therefore, jhp0951 status is an independent factor associated with the development of PUD, and jhp0945 may predict the future development of GC in patients with CAG and is considered to be the best candidate disease marker for H. pylori-related diseases. [ABSTRACT FROM AUTHOR]- Published
- 2015
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18. Protective role of fingolimod (FTY720) in rats subjected to subarachnoid hemorrhage.
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Xu, Hao-Liang, Pelligrino, Dale A., Paisansathan, Chanannait, and Testai, Fernando D.
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SUBARACHNOID hemorrhage , *ENDOVASCULAR surgery , *FINGOLIMOD , *RHODAMINES , *LEUCOCYTES - Abstract
Background: Subarachnoid hemorrhage (SAH) is a neurological emergency with limited pharmacological treatment options. Inflammation is increasingly recognized as a key pathogenic contributor to brain injury in this condition. In the present study, we examined the neuroprotective effects of the immunomodulatory agent, fingolimod, in rats subjected to SAH. Methods: We utilized an endovascular rat perforation model of SAH. Animals were divided into four groups: (1) sham-vehicle; (2) sham-fingolimod; (3) SAH-vehicle; and (4) SAH-fingolimod. Rats received either vehicle solution or fingolimod (0.5 mg/kg) intraperitoneally 3 hours after sham surgery or SAH. A closed cranial window and intravital microscope system was used at 48 hours to assess neuroinflammation, which was represented by rhodamine-6Glabeled leukocyte trafficking in pial venules, and pial arteriolar dilating responses to a variety of vasodilators, including hypercapnia, and topically-applied acetylcholine, adenosine, and S-nitroso-N-acetyl penicillamine. In addition, motor-sensory function was evaluated. Results: Compared to sham-vehicle rats, SAH-vehicle animals displayed a four-times greater increase in pial venular intraluminal leukocyte adhesion. Treatment with fingolimod largely reduced the intravascular leukocyte adhesion. Vehicle-treated SAH animals displayed a significant decrease in pial arteriolar responses to all the vasodilators tested and vascular reactivity was preserved, to a significant degree, in the presence of fingolimod. In addition, neurological scores obtained at 48 hours post-SAH indicated significant neurological deficits in the vehicle-treated group (versus sham-vehicle surgical control). Those deficiencies were partially reduced by fingolimod (P < 0.0001 compared to the vehicle-treated SAH group). Conclusions: Treatment of rats with fingolimod was associated with a marked limitation in the intravascular adhesion of leukocytes to pial venules, preserved pial arteriolar dilating function, and improved neurological outcome in rats subjected to SAH. [ABSTRACT FROM AUTHOR]
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- 2015
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19. Protective role of fingolimod (FTY720) in rats subjected to subarachnoid hemorrhage.
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Hao-Liang Xu, Pelligrino, Dale A., Paisansathan, Chanannait, and Testai, Fernando D.
- Subjects
- *
FINGOLIMOD , *LABORATORY rats , *SUBARACHNOID hemorrhage , *NEUROPROTECTIVE agents , *RHODAMINES , *LEUCOCYTES - Abstract
Background Subarachnoid hemorrhage (SAH) is a neurological emergency with limited pharmacological treatment options. Inflammation is increasingly recognized as a key pathogenic contributor to brain injury in this condition. In the present study, we examined the neuroprotective effects of the immunomodulatory agent, fingolimod, in rats subjected to SAH. Methods We utilized an endovascular rat perforation model of SAH. Animals were divided into four groups: (1) sham-vehicle; (2) sham-fingolimod; (3) SAH-vehicle; and (4) SAH-fingolimod. Rats received either vehicle solution or fingolimod (0.5 mg/kg) intraperitoneally 3 hours after sham surgery or SAH. A closed cranial window and intravital microscope system was used at 48 hours to assess neuroinflammation, which was represented by rhodamine-6G-labeled leukocyte trafficking in pial venules, and pial arteriolar dilating responses to a variety of vasodilators, including hypercapnia, and topically-applied acetylcholine, adenosine, and S-nitroso-N-acetyl penicillamine. In addition, motor-sensory function was evaluated. Results Compared to sham-vehicle rats, SAH-vehicle animals displayed a four-times greater increase in pial venular intraluminal leukocyte adhesion. Treatment with fingolimod largely reduced the intravascular leukocyte adhesion. Vehicle-treated SAH animals displayed a significant decrease in pial arteriolar responses to all the vasodilators tested and vascular reactivity was preserved, to a significant degree, in the presence of fingolimod. In addition, neurological scores obtained at 48 hours post-SAH indicated significant neurological deficits in the vehicle-treated group (versus sham-vehicle surgical control). Those deficiencies were partially reduced by fingolimod (P < 0.0001 compared to the vehicle-treated SAH group). Conclusions Treatment of rats with fingolimod was associated with a marked limitation in the intravascular adhesion of leukocytes to pial venules, preserved pial arteriolar dilating function, and improved neurological outcome in rats subjected to SAH. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
20. Long term follow up and retrospective study on 533 gastric cancer cases.
- Author
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Wei-Juan Zeng, Wen-Qin Hu, Lin-Wei Wang, Shu-Guang Yan, Jian-Ding Li, Hao-Liang Zhao, Chun-Wei Peng, Gui-Fang Yang, and Yan Li
- Subjects
STOMACH cancer ,CANCER-related mortality ,KAPLAN-Meier estimator ,PROGNOSIS ,ONCOLOGIC surgery ,CANCER invasiveness - Abstract
Background Gastric cancer (GC) is the third leading cause of cancer death in China and the outcome of GC patients is poor. The aim of the research is to study the prognostic factors of gastric cancer patients who had curative intent or palliative resection, completed clinical database and follow-up. Methods This retrospective study analyzed 533 GC patients from three tertiary referral teaching hospitals from January 2004 to December 2010 who had curative intent or palliative resection, complete clinical database and follow-up information. The GC-specific overall survival (OS) status was determined by the Kaplan-Meier method, and univariate analysis was conducted to identify possible factors for survival. Multivariate analysis using the Cox proportional hazard model and a forward regression procedure was conducted to define independent prognostic factors. Results By the last follow-up, the median follow-up time of 533 GC patients was 38.6 mo (range 6.9- 100.9 mo), and the median GC-specific OS was 25.3 mo (95% CI: 23.1-27.4 mo). The estimated 1-, 2-, 3- and 5-year GC-specific OS rates were 78.4%, 61.4%, 53.3% and 48.4%, respectively. Univariate analysis identified the following prognostic factors: hospital, age, gender, cancer site, surgery type, resection type, other organ resection, HIPEC, LN status, tumor invasion, distant metastases, TNM stage, postoperative SAE, systemic chemotherapy and IP chemotherapy. In multivariate analysis, seven factors were identified as independent prognostic factors for long term survival, including resection type, HIPEC, LN status, tumor invasion, distant metastases, postoperative SAE and systemic chemotherapy. Conclusions Resection type, HIPEC, postoperative SAE and systemic chemotherapy are four independent prognostic factors that could be intervened for GC patients for improving survival. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
21. A gene expression restriction network mediated by sense and antisense Alu sequences located on protein-coding messenger RNAs.
- Author
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Kung-Hao Liang and Chau-Ting Yeh
- Subjects
- *
GENE expression , *RETROTRANSPOSONS , *MESSENGER RNA , *ANTISENSE genetics , *GENETIC transcription , *HUMAN genome , *GENE expression profiling - Abstract
Background: Alus are primate-specific retrotransposons which account for 10.6% of the human genome. A large number of protein-coding mRNAs are encoded with sense or antisense Alus in the un-translated regions. Results: We postulated that mRNAs carrying Alus in the two opposite directions can generate double stranded RNAs, capable of regulating the levels of other Alu-carrying mRNAs post-transcriptionally. A gene expression profiling assay showed that the levels of antisense and sense Alus-carrying mRNAs were suppressed in a reversible manner by over-expression of exogenous sense and antisense Alus derived from mRNAs (Family-wise error rate P= 0.0483 and P < 0.0001 respectively). Screening through human mRNAs on the NCBI-RefSeq database, it was found that sense and antisense Alu-carrying transcripts were enriched in distinct cellular functions. Antisense Alu-carrying genes were particularly enriched in neurological and developmental processes, while sense Alu-carrying genes were enriched in immunological functions. Conclusions: Taken together, we proposed a novel Alu-mediated regulation network capable of stabilizing Alu-carrying mRNA levels in different cell types and restricting the activated expression levels of protein-coding, Alu-carrying mRNAs. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
22. Clinical application of human β-defensin and CD14 gene polymorphism in evaluating the status of chronic inflammation.
- Author
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Loo, Wings T. Y., Lan-jun Bai, Chang-bin Fan, Yuan Yue, Yi-ding Dou, Min Wang, Hao Liang, Cheung, Mary N. B., Chow, Louis W. C., Jin-le Li, Ye Tian, and Liu Qing
- Subjects
PERIODONTITIS ,DIABETES ,SMOKING ,LIPOPOLYSACCHARIDES ,SINGLE nucleotide polymorphisms ,INFLAMMATION - Abstract
Background: Periodontitis is a common disease that affects the periodontal tissue supporting the teeth. This disease is attributed to multiple risk factors, including diabetes, cigarette smoking, alcohol, pathogenic microorganisms, genetics and others. Human beta-defensin-1 (hBD-1) is a cationic antimicrobial peptide with cysteine-rich ß-sheets and broad-spectrum antimicrobial activity. CD14 is a protein involved in the detection of bacterial lipopolysaccharide (LPS) and has also been associated with periodontitis. This study investigates the single nucleotide polymorphic (SNP) region, -1654(V38I), of the human beta-defensin-1 (hBD-1) gene as well as the -159 region of the CD14 gene in subjects with chronic periodontitis. Methods: Blood samples from periodontally healthy subjects and periodontitis patients were obtained. DNA was extracted from the blood and was used to perform restriction digest at the polymorphic G1654A site of DEFB1 with the enzyme HincII. The polymorphic site 159TT of CD14 was digested with the enzyme AvaII. Enzyme-linked immunosorbent assay (ELISA) was performed on soluble samples to determine the protein expressions. Results: The control and patient groups expressed 35% and 38% 1654 A/A genotype of DEFB1, respectively. The A allele frequency of the control group was 40%, while the patient blood group was 54%. The mean hBD-1 protein levels of the control and patient samples were 102.83 pg/mL and 252.09 pg/mL, respectively. The genotype distribution of CD14 in healthy subjects was 16% for C/C, 26% for T/T and 58% for C/T. The genotype frequencies of CD14 in periodontitis patients were 10% for C/C, 43% for T/T and 47% for C/T. The CD14 protein expression determined by ELISA showed a mean protein level of the control samples at 76.28ng/mL and the patient blood samples at 179.27ng/mL with a p value of 0.001. Our study demonstrated that patients suffering from chronic periodontitis present more commonly with the 1654A/A genotype on the DEFB1 gene and the 159T/T genotype on the CD14 gene. Conclusions: This study purely investigated the association between periodontitis and one polymorphic site on both DEFB1 and CD14 gene, with the purpose of expanding knowledge for the future development in diagnostic markers or therapeutic interventions to combat this disease. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
23. Gene polymorphism and protein of human proand anti-inflammatory cytokines in Chinese healthy subjects and chronic periodontitis patients.
- Author
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Wings TY Loo, Chang-bin Fan, Lan-jun Bai, Yuan Yue, Yi-ding Dou, Min Wang, Hao Liang, Cheung, Mary N. B., Chow, Louis W. C., Jin-le Li, Ye Tian, and Liu Qing
- Subjects
PERIODONTAL disease ,CYTOKINES ,ANTI-inflammatory agents ,PERIODONTITIS ,ENZYME-linked immunosorbent assay - Abstract
Background: Periodontal disease is thought to arise from the interaction of various factors, including the susceptibility of the host, the presence of pathogenic organisms, and the absence of beneficial species. The genetic factors may play a significant role in the risk of periodontal diseases. Cytokines initiate, mediate and control immune and inflammatory responses. The aim of this study is to compare genotypes and soluble protein of pro and anti-inflammatory cytokines (IL-1α, IL-1β, IL-6, IFN-,γ IL-10, TNF-α and IL-4) in subjects with or free of chronic periodontitis. Methods: A total of 1,290 Chinese subjects were recruited to this clinical trial: 850 periodontally healthy controls and 440 periodontal patients. All subjects were free of systemic diseases. Oral examinations were performed, and the following parameters were recorded for each subject: supragingival/subgingival calculus, gingival recession, bleeding on probing (BOP), probing depth (PD), clinical attachment loss (CAL), gingival recession and tooth mobility. The peripheral blood samples were collected for genetic and enzyme linked immunosorbent assay (ELISA) analysis. Restriction enzymes were used for digestion of amplified fragments of IL-1α, IL-1β, IL-6, IFN-,γ IL-10, TNF-α and IL-4. Results: The protein expressions of patient and control samples for IL-1α, IL-1β, IL-6, TNF-α, IFN-γ, IL-10, and IL-4 measured by ELISA confirmed a statistically significant difference (p < 0.001). The digestion of fragments of various genes showed that the pro-inflammatory cytokines IL-1a and TNF-α, and the anti-inflammatory cytokines IL-4 and IL-10 demonstrated a correlation with chronic inflammation in patients (X²: p < 0.001). The remaining genes investigated in patients and healthy subjects (IL-1β, IL-6, IFN- γand IL-10) did not show any significant difference. Conclusions: The cytokine gene polymorphisms may be used as a marker for periodontitis susceptibility, clinical behaviour and severity. This detection offers early diagnosis and induction of prophylaxis to other family members against disease progression. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
24. Comparing serum levels of cardiac biomarkers in cancer patients receiving chemotherapy and subjects with chronic periodontitis.
- Author
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Loo, Wings T. Y., Yuan Yue, Chang-bin Fan, Lan-jun Bai, Yi-ding Dou, Min Wang, Hao Liang, Cheung, Mary N. B., Chow, Louis W. C., Jin-le Li, Ye Tian, and Liu Qing
- Subjects
PERIODONTITIS ,INFLAMMATION ,ANTINEOPLASTIC agents ,ANTHRACYCLINES ,CORONARY disease ,CANCER - Abstract
Background: Chronic periodontitis (CP) is a chronic inflammation associated with elevations of several inflammatory and cardiac markers. Studies implicated CP as one of the etiologies in coronary heart disease (CHD). Cardiotoxicity is a major complication of anticancer drugs, including anthracyclines and 5-fluorouracil (5FU). The most severe cardiac complications are heart failure, arrhythmia and coronary heart disease (CHD). In this study, we compared the level of inflammatory factors and cardiac markers between chronic periodontitis patients and cancer patients receiving chemotherapy. Methods: 108 blood samples of periodontally healthy subjects were obtained on random from Hong Kong Red Cross, and these represented the controlled population. Forty-four patients diagnosed with chronic periodontitis were recruited from the West China Hospital of Stomatology, Sichuan University. They have received scaling and root planning with mean pocket depths of 6.05 mm. Thirty breast cancer patients diagnosed with invasive ductal carcinoma from UNIMED Medical Institute, Hong Kong gave consent to participate in this study. They received 4 cycles of 500mg/m2 5-fluorouracil, 75 mg/m2 epirubicin and 500mg/m2 cyclophosphamide at a 3-week interval between each cycle. Peripheral venous blood from each group was taken for measurement of blood cells, inflammatory marker (P-selectin, high sensitvity C-reactive protein) and cardiac markers (troponin T; troponin I; N-terminal pro brain natriuretic peptide (Nt-proBNP) and Lactate dehydrogenase (LDH). Results: The lymphocyte count was higher (p < 0.05) in periodontitis patients than the other two groups, and more neutrophils (p < 0.05) were seen in cancer patients receiving chemotherapy. The two test groups demonstrated higher levels (p < 0.01) of inflammatory and cardiac markers than the control group. Conclusions: The elevated cardiac markers found in periodontitis patients suggested that they may carry potential risks in developing cardiac lesions. Troponin T, troponin I, pro-BNP, LDH and high sensitvity C-reactive protein may be used as markers to monitor cardiac lesions in chronic inflammatory patients. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
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25. Estimation of cancer incidence and mortalityattributable to alcohol drinking in china.
- Author
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Hao Liang, Jianbing Wang, Huijuan Xiao, Ding Wang, Wenqiang Wei, Youlin Qiao, and Boffetta, Paolo
- Subjects
- *
CANCER research , *ALCOHOLISM , *PEOPLE with alcoholism , *PUBLIC health - Abstract
Background: Cancer constitutes a serious burden of disease worldwide and has become the second leading cause of death in China. Alcohol consumption is causally associated with the increased risk of certain cancers. Due to the current lack of data and the imperative need to guide policymakers on issues of cancer prevention and control, we aim to estimate the role of alcohol on the cancer burden in China in 2005. Methods: We calculated the proportion of cancers attributable to alcohol use to estimate the burden of alcoholrelated cancer. The population attributable fraction was calculated based on the assumption of no alcohol drinking. Data on alcohol drinking prevalence were from two large-scale national surveys of representative samples of the Chinese population. Data on relative risk were obtained from meta-analyses and large-scale studies. Results: We found that a total of 78,881 cancer deaths were attributable to alcohol drinking in China in 2005, representing 4.40% of all cancers (6.69% in men, 0.42% in women). The corresponding figure for cancer incidence was 93,596 cases (3.63% of all cancer cases). Liver cancer was the main alcohol-related cancer, contributing more than 60% of alcohol-related cancers. Conclusions: Particular attention needs to be paid to the harm of alcohol as well as its potential benefits when making public health recommendations on alcohol drinking. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
26. Long term follow up and retrospective study on 533 gastric cancer cases.
- Author
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Zeng, Wei-Juan, Hu, Wen-Qin, Wang, Lin-Wei, Yan, Shu-Guang, Li, Jian-Ding, Zhao, Hao-Liang, Peng, Chun-Wei, Yang, Gui-Fang, and Li, Yan
- Abstract
Background: Gastric cancer (GC) is the third leading cause of cancer death in China and the outcome of GC patients is poor. The aim of the research is to study the prognostic factors of gastric cancer patients who had curative intent or palliative resection, completed clinical database and follow-up.Methods: This retrospective study analyzed 533 GC patients from three tertiary referral teaching hospitals from January 2004 to December 2010 who had curative intent or palliative resection, complete clinical database and follow-up information. The GC-specific overall survival (OS) status was determined by the Kaplan-Meier method, and univariate analysis was conducted to identify possible factors for survival. Multivariate analysis using the Cox proportional hazard model and a forward regression procedure was conducted to define independent prognostic factors.Results: By the last follow-up, the median follow-up time of 533 GC patients was 38.6 mo (range 6.9-100.9 mo), and the median GC-specific OS was 25.3 mo (95% CI: 23.1-27.4 mo). The estimated 1-, 2-, 3- and 5-year GC-specific OS rates were 78.4%, 61.4%, 53.3% and 48.4%, respectively. Univariate analysis identified the following prognostic factors: hospital, age, gender, cancer site, surgery type, resection type, other organ resection, HIPEC, LN status, tumor invasion, distant metastases, TNM stage, postoperative SAE, systemic chemotherapy and IP chemotherapy. In multivariate analysis, seven factors were identified as independent prognostic factors for long term survival, including resection type, HIPEC, LN status, tumor invasion, distant metastases, postoperative SAE and systemic chemotherapy.Conclusions: Resection type, HIPEC, postoperative SAE and systemic chemotherapy are four independent prognostic factors that could be intervened for GC patients for improving survival. [ABSTRACT FROM AUTHOR]- Published
- 2014
- Full Text
- View/download PDF
27. Prognostic and clinical significance of claudin-4 in gastric cancer: a meta-analysis.
- Author
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Liu JX, Wei ZY, Chen JS, Lu HC, Hao L, and Li WJ
- Subjects
- Disease Progression, Humans, Lymphatic Metastasis, Prognosis, Stomach Neoplasms diagnosis, Stomach Neoplasms mortality, Survival Rate, Biomarkers metabolism, Claudin-4 metabolism, Stomach Neoplasms metabolism
- Abstract
Background: The current reports on the association of claudin-4 expression with gastric cancer outcome were inconsistent. Thus, we conducted a meta-analysis to assess the association of claudin-4 expression with the prognosis and clinical parameters more precisely., Methods: Systematic searches on PubMed, Embase, and Cochrane Library prior to December 2014 were performed. The pooled hazard ratio (HR) with its 95 % confidence interval (95 %CI) was used to assess the prognostic value of claudin-4 expression with gastric cancer patients, and the pooled odds ratio (OR) with its 95 %CI was used to assess the association with clinical parameters., Results: Nine studies with a total of 1265 gastric cancer patients were included. Overall, the pooled results showed that over-expression of claudin-4 was associated with a poor survival in gastric cancer patients (HR: 2.01, 95 % CI: 1.62-2.50). Over-expression of claudin-4 was also associated with advanced stage (OR: 1.96, 95 % CI: 1.08-3.56) and lymphoid node metastasis (OR: 1.72, 95 % CI: 1.05-2.81) of gastric cancer patients. No significant publication bias was found among the studies (P > 0.05)., Conclusions: This meta-analysis shows that over-expression of claudin-4 is associated with progress of gastric cancer and poor prognosis of gastric cancer patients.
- Published
- 2015
- Full Text
- View/download PDF
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