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Your search keyword '"Gurumurthy, Channabasavaiah B."' showing total 12 results
Start Over Author "Gurumurthy, Channabasavaiah B." Publisher biomed central
12 results on '"Gurumurthy, Channabasavaiah B."'

3. CD4+ effector T cells accelerate Alzheimer’s disease in mice

Author

Machhi, Jatin, Yeapuri, Pravin, Lu, Yaman, Foster, Emma, Chikhale, Rupesh, Herskovitz, Jonathan, Namminga, Krista L., Olson, Katherine E., Abdelmoaty, Mai Mohamed, Gao, Ju, Quadros, Rolen M., Kiyota, Tomomi, Jingjing, Liang, Kevadiya, Bhavesh D., Wang, Xinglong, Liu, Yutong, Poluektova, Larisa Y., Gurumurthy, Channabasavaiah B., Mosley, R. Lee, and Gendelman, Howard E.

Published
2021
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4. Response to correspondence on “Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation”

Author

Gurumurthy, Channabasavaiah B., O’Brien, Aidan R., Quadros, Rolen M., Adams, Jr, John, Alcaide, Pilar, Ayabe, Shinya, Ballard, Johnathan, Batra, Surinder K., Beauchamp, Marie-Claude, Becker, Kathleen A., Bernas, Guillaume, Brough, David, Carrillo-Salinas, Francisco, Chan, Wesley, Chen, Hanying, Dawson, Ruby, DeMambro, Victoria, D’Hont, Jinke, Dibb, Katharine, Eudy, James D., Gan, Lin, Gao, Jing, Gonzales, Amy, Guntur, Anyonya, Guo, Huiping, Harms, Donald W., Harrington, Anne, Hentges, Kathryn E., Humphreys, Neil, Imai, Shiho, Ishii, Hideshi, Iwama, Mizuho, Jonasch, Eric, Karolak, Michelle, Keavney, Bernard, Khin, Nay-Chi, Konno, Masamitsu, Kotani, Yuko, Kunihiro, Yayoi, Lakshmanan, Imayavaramban, Larochelle, Catherine, Lawrence, Catherine B., Li, Lin, Lindner, Volkhard, Liu, Xian-De, Lopez-Castejon, Gloria, Loudon, Andrew, Lowe, Jenna, Jerome-Majeweska, Loydie, Matsusaka, Taiji, Miura, Hiromi, Miyasaka, Yoshiki, Morpurgo, Benjamin, Motyl, Katherine, Nabeshima, Yo-ichi, Nakade, Koji, Nakashiba, Toshiaki, Nakashima, Kenichi, Obata, Yuichi, Ogiwara, Sanae, Ouellet, Mariette, Oxburgh, Leif, Piltz, Sandra, Pinz, Ilka, Ponnusamy, Moorthy P., Ray, David, Redder, Ronald J., Rosen, Clifford J., Ross, Nikki, Ruhe, Mark T., Ryzhova, Larisa, Salvador, Ane M., Alam, Sabrina Shameen, Sedlacek, Radislav, Sharma, Karan, Smith, Chad, Staes, Katrien, Starrs, Lora, Sugiyama, Fumihiro, Takahashi, Satoru, Tanaka, Tomohiro, Trafford, Andrew, Uno, Yoshihiro, Vanhoutte, Leen, Vanrockeghem, Frederique, Willis, Brandon J., Wright, Christian S., Yamauchi, Yuko, Yi, Xin, Yoshimi, Kazuto, Zhang, Xuesong, Zhang, Yu, Ohtsuka, Masato, Das, Satyabrata, Garry, Daniel J., Hochepied, Tino, Thomas, Paul, Parker-Thornburg, Jan, Adamson, Antony D., Yoshiki, Atsushi, Schmouth, Jean-Francois, Golovko, Andrei, Thompson, William R., Lloyd, K. C. Kent, Wood, Joshua A., Cowan, Mitra, Mashimo, Tomoji, Mizuno, Seiya, Zhu, Hao, Kasparek, Petr, Liaw, Lucy, Miano, Joseph M., and Burgio, Gaetan

Published
2021
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5. Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

Author

Gurumurthy, Channabasavaiah B., O’Brien, Aidan R., Quadros, Rolen M., Adams, Jr, John, Alcaide, Pilar, Ayabe, Shinya, Ballard, Johnathan, Batra, Surinder K., Beauchamp, Marie-Claude, Becker, Kathleen A., Bernas, Guillaume, Brough, David, Carrillo-Salinas, Francisco, Chan, Wesley, Chen, Hanying, Dawson, Ruby, DeMambro, Victoria, D’Hont, Jinke, Dibb, Katharine M., Eudy, James D., Gan, Lin, Gao, Jing, Gonzales, Amy, Guntur, Anyonya R., Guo, Huiping, Harms, Donald W., Harrington, Anne, Hentges, Kathryn E., Humphreys, Neil, Imai, Shiho, Ishii, Hideshi, Iwama, Mizuho, Jonasch, Eric, Karolak, Michelle, Keavney, Bernard, Khin, Nay-Chi, Konno, Masamitsu, Kotani, Yuko, Kunihiro, Yayoi, Lakshmanan, Imayavaramban, Larochelle, Catherine, Lawrence, Catherine B., Li, Lin, Lindner, Volkhard, Liu, Xian-De, Lopez-Castejon, Gloria, Loudon, Andrew, Lowe, Jenna, Jerome-Majewska, Loydie A., Matsusaka, Taiji, Miura, Hiromi, Miyasaka, Yoshiki, Morpurgo, Benjamin, Motyl, Katherine, Nabeshima, Yo-ichi, Nakade, Koji, Nakashiba, Toshiaki, Nakashima, Kenichi, Obata, Yuichi, Ogiwara, Sanae, Ouellet, Mariette, Oxburgh, Leif, Piltz, Sandra, Pinz, Ilka, Ponnusamy, Moorthy P., Ray, David, Redder, Ronald J., Rosen, Clifford J., Ross, Nikki, Ruhe, Mark T., Ryzhova, Larisa, Salvador, Ane M., Alam, Sabrina Shameen, Sedlacek, Radislav, Sharma, Karan, Smith, Chad, Staes, Katrien, Starrs, Lora, Sugiyama, Fumihiro, Takahashi, Satoru, Tanaka, Tomohiro, Trafford, Andrew W., Uno, Yoshihiro, Vanhoutte, Leen, Vanrockeghem, Frederique, Willis, Brandon J., Wright, Christian S., Yamauchi, Yuko, Yi, Xin, Yoshimi, Kazuto, Zhang, Xuesong, Zhang, Yu, Ohtsuka, Masato, Das, Satyabrata, Garry, Daniel J., Hochepied, Tino, Thomas, Paul, Parker-Thornburg, Jan, Adamson, Antony D., Yoshiki, Atsushi, Schmouth, Jean-Francois, Golovko, Andrei, Thompson, William R., Lloyd, K. C. Kent, Wood, Joshua A., Cowan, Mitra, Mashimo, Tomoji, Mizuno, Seiya, Zhu, Hao, Kasparek, Petr, Liaw, Lucy, Miano, Joseph M., and Burgio, Gaetan

Published
2019
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8. Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins

Author

Quadros, Rolen M., Miura, Hiromi, Harms, Donald W., Akatsuka, Hisako, Sato, Takehito, Aida, Tomomi, Redder, Ronald, Richardson, Guy P., Inagaki, Yutaka, Sakai, Daisuke, Buckley, Shannon M., Seshacharyulu, Parthasarathy, Batra, Surinder K., Behlke, Mark A., Zeiner, Sarah A., Jacobi, Ashley M., Izu, Yayoi, Thoreson, Wallace B., Urness, Lisa D., Mansour, Suzanne L., Ohtsuka, Masato, and Gurumurthy, Channabasavaiah B.

Published
2017
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9. Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new.

Author

Sato, Masahiro, Ohtsuka, Masato, Watanabe, Satoshi, and Gurumurthy, Channabasavaiah B.

Subjects
GENOME editing, NUCLEIC acids, CRISPRS, EMBRYONIC stem cells, MICROINJECTIONS
Abstract

In the recent years, sequence-specific nucleases such as ZFNs, TALENs, and CRISPR/Cas9 have revolutionzed the fields of animal genome editing and transgenesis. However, these new techniques require microinjection to deliver nucleic acids into embryos to generate gene-modified animals. Microinjection is a delicate procedure that requires sophisticated equipment and highly trained and experienced technicians. Though over a dozen alternate approaches for nucleic acid delivery into embryos were attempted during the pre-CRISPR era, none of them became routinely used as microinjection. The addition of CRISPR/Cas9 to the genome editing toolbox has propelled the search for novel delivery approaches that can obviate the need for microinjection. Indeed, some groups have recently developed electroporation-based methods that have the potential to radically change animal transgenesis. This review provides an overview of the old and new delivery methods, and discusses various strategies that were attempted during the last three decades. In addition, several of the methods are re-evaluated with respect to their suitability to deliver genome editing components, particularly CRISPR/Cas9, to embryos. [ABSTRACT FROM AUTHOR]

Published
2016
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10. One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).

Author

Masato Ohtsuka, Hiromi Miura, Keiji Mochida, Michiko Hirose, Ayumi Hasegawa, Atsuo Ogura, Ryuta Mizutani, Minoru Kimura, Ayako Isotani, Masahito Ikawa, Masahiro Sato, and Gurumurthy, Channabasavaiah B.

Subjects
TRANSGENIC animals, GENE expression, TRANSGENE expression, GENETIC research
Abstract

Background: The pronuclear injection (PI) is the simplest and widely used method to generate transgenic (Tg) mice. Unfortunately, PI-based Tg mice show uncertain transgene expression due to random transgene insertion in the genome, usually with multiple copies. Thus, typically at least three or more Tg lines are produced by injecting over 200 zygotes and the best line/s among them are selected through laborious screening steps. Recently, we developed technologies using Cre-loxP system that allow targeted insertion of single-copy transgene into a predetermined locus through PI. We termed the method as PI-based Targeted Transgenesis (PITT). A similar method using PhiC31-attP/B system was reported subsequently. Results: Here, we developed an improved-PITT (i-PITT) method by combining Cre-loxP, PhiC31-attP/B and FLP-FRT systems directly under C57BL/6N inbred strain, unlike the mixed strain used in previous reports. The targeted Tg efficiency in the i-PITT typically ranged from 10 to 30%, with 47 and 62% in two of the sessions, which is by-far the best Tg rate reported. Furthermore, the system could generate multiple Tg mice simultaneously. We demonstrate that injection of up to three different Tg cassettes in a single injection session into as less as 181 zygotes resulted in production of all three separate Tg DNA containing targeted Tg mice. Conclusions: The i-PITT system offers several advantages compared to previous methods: multiplexing capability (i-PITT is the only targeted-transgenic method that is proven to generate multiple different transgenic lines simultaneously), very high efficiency of targeted-transgenesis (up to 62%), significantly reduces animal numbers in mouse-transgenesis and the system is developed under C57BL/6N strain, the most commonly used pure genetic background. Further, the i-PITT system is freely accessible to scientific community. [ABSTRACT FROM AUTHOR]

Published
2015
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12. One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).

Author

Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, and Gurumurthy CB

Subjects
Animals, Embryonic Stem Cells, Female, Injections methods, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Gene Targeting, Gene Transfer Techniques
Abstract

Background: The pronuclear injection (PI) is the simplest and widely used method to generate transgenic (Tg) mice. Unfortunately, PI-based Tg mice show uncertain transgene expression due to random transgene insertion in the genome, usually with multiple copies. Thus, typically at least three or more Tg lines are produced by injecting over 200 zygotes and the best line/s among them are selected through laborious screening steps. Recently, we developed technologies using Cre-loxP system that allow targeted insertion of single-copy transgene into a predetermined locus through PI. We termed the method as PI-based Targeted Transgenesis (PITT). A similar method using PhiC31-attP/B system was reported subsequently., Results: Here, we developed an improved-PITT (i-PITT) method by combining Cre-loxP, PhiC31-attP/B and FLP-FRT systems directly under C57BL/6N inbred strain, unlike the mixed strain used in previous reports. The targeted Tg efficiency in the i-PITT typically ranged from 10 to 30%, with 47 and 62% in two of the sessions, which is by-far the best Tg rate reported. Furthermore, the system could generate multiple Tg mice simultaneously. We demonstrate that injection of up to three different Tg cassettes in a single injection session into as less as 181 zygotes resulted in production of all three separate Tg DNA containing targeted Tg mice., Conclusions: The i-PITT system offers several advantages compared to previous methods: multiplexing capability (i-PITT is the only targeted-transgenic method that is proven to generate multiple different transgenic lines simultaneously), very high efficiency of targeted-transgenesis (up to 62%), significantly reduces animal numbers in mouse-transgenesis and the system is developed under C57BL/6N strain, the most commonly used pure genetic background. Further, the i-PITT system is freely accessible to scientific community.

Published
2015
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