1. Melatonin-loaded lipid-core nanocapsules protect against lipid peroxidation caused by paraquat through increased SOD expression in Caenorhabditis elegans
- Author
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Mariele Feiffer Charão, Vera Lucia Eifler-Lima, Silvia Stanisçuaski Guterres, Natália Brucker, Karina Paese, Adriana Raffin Pohlmann, Solange Cristina Garcia, and Gabriela Göethel
- Subjects
Paraquat ,Antioxidant ,Thiobarbituric acid ,medicine.medical_treatment ,Drug Compounding ,Pharmacology ,Nanocapsules ,Antioxidants ,Superoxide dismutase ,Lipid peroxidation ,Melatonin ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,lcsh:RA1190-1270 ,medicine ,TBARS ,Animals ,Pharmacology (medical) ,Particle Size ,Caenorhabditis elegans ,030304 developmental biology ,lcsh:Toxicology. Poisons ,0303 health sciences ,Drug Carriers ,biology ,Superoxide Dismutase ,Research ,lcsh:RM1-950 ,Lipids ,lcsh:Therapeutics. Pharmacology ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,C. elegans ,Herbicide ,Lipid Peroxidation ,medicine.drug - Abstract
Background Melatonin has been described in the literature as a potent antioxidant. However, melatonin presents variable, low bioavailability and a short half-life. The use of polymeric nanoparticulated systems has been proposed for controlled release. Thus, the purpose of this study was to investigate the action of melatonin-loaded lipid-core nanocapsules (Mel-LNC) in the antioxidant system of Caenorhabditis elegans, and the possible protective effect of this formulation against lipid peroxidation caused by paraquat (PQ). Methods The suspensions were prepared by interfacial deposition of the polymer and were physiochemically characterized. C. elegans N2 wild type and transgenic worm CF1553, muls84 [sod-3p::gfp; rol6(su1006)] were obtained from the Caenorhabditis Genetics Center (CGC). The worms were divided into 5 groups: Control, PQ 0.5 mM, PQ 0.5 mM + Mel-LNC 10 μg/mL, PQ + unloaded lipid-core nanocapsules (LNC), and PQ + free melatonin (Mel) 10 μg/mL. The lipid peroxidation was assessed through thiobarbituric acid (TBARS) levels and the fluorescence levels of the transgenic worms expressing GFP were measured. Results The LNC and Mel-LNC presented a bluish-white liquid, with pH values of 5.56 and 5.69, respectively. The zeta potential was − 6.4 ± 0.6 and − 5.2 ± 0.2, respectively. The mean particle diameter was 205 ± 4 nm and 203 ± 3 nm, respectively. The total melatonin content was 0.967 mg/ml. The TBARS levels were significantly higher in the PQ group when compared to the control group (p p C. elegans from lipid peroxidation caused by PQ and this was not observed when free melatonin was used. Moreover, Mel-LNC increased the fluorescence intensity of the transgenic strain that encodes the antioxidant enzyme SOD-3, demonstrating a possible mechanism of protection from PQ-induced damage. Conclusion These findings demonstrated that melatonin, when associated with nanocapsules, had improved antioxidant properties and the protective activity against PQ-induced lipid peroxidation could be associated with the activation of antioxidant enzymes by Mel-LNC in C. elegans.
- Published
- 2019