20 results on '"Fan, Feng"'
Search Results
2. Systemic immune inflammation index and system inflammation response index are potential biomarkers of atrial fibrillation among the patients presenting with ischemic stroke
- Author
-
Lin, Kai-bin, Fan, Feng-hua, Cai, Ming-qi, Yu, Yin, Fu, Chuan-liang, Ding, Lu-yue, Sun, Yu-dong, Sun, Jia-wen, Shi, Yong-wang, Dong, Zhi-feng, Yuan, Min-Jie, Li, Shuai, Wang, Yan-peng, Chen, Kan-kai, Zhu, Ji-ni, Guo, Xin-wei, Zhang, Xue, Zhao, Yu-wu, Li, Jing-bo, and Huang, Dong
- Published
- 2022
- Full Text
- View/download PDF
3. General practitioners’ perceptions of their practice of evidence-based chronic disease prevention interventions: a quantitative study in Shanghai, China
- Author
-
Fan, Feng, Wang, Zhaoxin, Yu, Dehua, Chen, Chen, Shen, Delei, Yu, Zhaohu, Liu, Xin, Zhou, Huining, and Shi, Jianwei
- Published
- 2020
- Full Text
- View/download PDF
4. The correlation between patent foramen ovale and brain ischemia in plateau residents
- Author
-
Xiaoting Xie, Fan Feng, Gang Liu, Fei Liu, Zhao Feng, and Changju Xue
- Subjects
Adult ,Male ,medicine.medical_specialty ,Ischemia ,Foramen Ovale, Patent ,Tibet ,Brain Ischemia ,Correlation ,Lesion ,Brain ischemia ,Internal medicine ,medicine ,Diseases of the circulatory (Cardiovascular) system ,Humans ,Stroke ,Angiology ,Chi-Square Distribution ,business.industry ,Altitude ,Incidence ,medicine.disease ,Magnetic Resonance Imaging ,Patent foramen ovale ,Cardiac surgery ,Echocardiography ,RC666-701 ,Case-Control Studies ,Cardiology ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Research Article - Abstract
Background It has been suggested that patent foramen ovale (PFO) contributes to the majority of cryptogenic stroke cases in young people, however, the direct link is still undetermined. Here we analyzed the correlation between PFO and brain ischemia lesions in a cohort of cases that were long-term residents in the plateau to provide solid evidence to support the causal relation between PFO and brain ischemia lesion or cryptogenic stroke. Methods Long-term residents with young age from Qinghai Plateau were recruited and separated by PFO positivity. Brain MRI was used to image 100 PFO positive cases and 100 healthy controls. The diameter of PFO was measured by echocardiography. The location, number and anterior/posterior circulation of ischemia lesions were also evaluated. The correlation between PFO (including positivity and diameter) and brain ischemia lesion (including positivity and other characteristics) was analyzed by chi-square test. Further, the chi-square test for the trend test was used to analyze the linear correlation between these groups. Results We found a strong correlation between the positivity of PFO and brain ischemia lesion, with 71% of PFO cases showing the presence of brain ischemia lesions, and only 19% for healthy controls (p Conclusion We found a convincing correlation between the positivity of PFO and brain ischemia lesion in residents of the plateau. Our findings provide another solid evidence of the direct causal relation between PFO and brain ischemia lesion.
- Published
- 2021
5. Robot-assisted orthopedic surgery in the treatment of adult degenerative scoliosis: a preliminary clinical report
- Author
-
Fan Feng, Yingchao Han, Quan Li, Lifeng Lao, Xiaosheng Yu, Xiuyuan Chen, Shurong Wang, Zhi Chen, Hongxing Shen, Zhipeng Tu, and Hao Chen
- Subjects
Adult ,Male ,medicine.medical_specialty ,lcsh:Diseases of the musculoskeletal system ,Robot ,Operative Time ,Blood Loss, Surgical ,Scoliosis ,03 medical and health sciences ,0302 clinical medicine ,lcsh:Orthopedic surgery ,Robotic Surgical Procedures ,Pedicle Screws ,Medicine ,Humans ,Orthopedics and Sports Medicine ,Orthopedic Procedures ,030212 general & internal medicine ,Adverse effect ,Pedicle screw ,Aged ,Retrospective Studies ,business.industry ,Degenerative scoliosis ,Retrospective cohort study ,Length of Stay ,Middle Aged ,medicine.disease ,Surgery ,lcsh:RD701-811 ,Treatment Outcome ,Internal fixators ,Radiological weapon ,Orthopedic surgery ,Female ,lcsh:RC925-935 ,business ,Complication ,Tomography, X-Ray Computed ,030217 neurology & neurosurgery ,Research Article - Abstract
Study design A single-institution, retrospective cohort study. Objective To compare the accuracy and short-term clinical outcomes of pedicle screw placement between robot-assisted (RA) and freehand (FH) technique in the treatment of adult degenerative scoliosis (ADS). Methods From February 2018 to October 2019, 97 adult patients with degenerative scoliosis admitted to our department were retrospectively reviewed. Thirty-one patients received robot-assisted pedicle screw placement (RA group), and 66 patients underwent freehand pedicle screw placement (FH group). Patient demographics and short-term clinical outcomes were recorded and compared between two groups. Gertzbein-Robbins grading system was adopted to evaluate the accuracy of pedicle screw placement by means of postoperative CT scan. Short-term clinical outcomes consist of operative time, intraoperative blood loss, length of hospital stay (LOS), radiological parameters, Scoliosis Research Society-22 (SRS-22) scores before the operation, 6 months after operation, adverse events, and revisions. Results The accuracy of screw placement was higher than that of the FH group (clinically acceptable 98.7% vs. 92.2%; P< 0.001). Intraoperative blood loss of the RA group was less than those in the FH group (499 vs. 573 ml; P < 0.001). Operative time (283.1 vs. 291.9 min; P = 0.31) and length of stay (12.8 vs. 13.7 days; P = 0.36) were compared between RA and FH groups. In terms of radiological parameters, both of groups were improved postoperatively. The SRS-22 scores at 6 months after operation from both groups were better than those before operation. For surgery-related complication, one case had pressure sores in the RA group while two cases developed dural tears in the FH group. No revision was required in both groups. Conclusion Combined with other surgical correction modalities, robot-assisted pedicle screw fixation is an effective and safe method of treating degenerative scoliosis. Due to its satisfactory surgical outcomes such as higher accuracy and less trauma, it provides a good alternative for clinical practice. Level of evidence 3.
- Published
- 2020
6. Increased NHE1 expression is targeted by specific inhibitor cariporide to sensitize resistant breast cancer cells to doxorubicin in vitro and in vivo
- Author
-
Hui Yang, Qi Chen, Yueqin Liu, Xiaolan Zhu, Wenlin Xu, and Fan Feng
- Subjects
0301 basic medicine ,Cancer Research ,Cell Survival ,Apoptosis ,Breast Neoplasms ,lcsh:RC254-282 ,Guanidines ,Metastasis ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Sensitivity ,In vivo ,Cell Line, Tumor ,MDR ,Genetics ,medicine ,Chemotherapy ,Animals ,Humans ,Doxorubicin ,Sulfones ,NHE-1 ,Cell Proliferation ,MCF-7/ADR ,Sodium-Hydrogen Exchanger 1 ,Cariporide ,Chemistry ,Cell growth ,Cell Cycle Checkpoints ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Immunohistochemistry ,Gene Expression Regulation, Neoplastic ,Disease Models, Animal ,030104 developmental biology ,Oncology ,Cell culture ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,Female ,medicine.drug ,Research Article - Abstract
Background The Na+/H+ exchanger (NHE1) plays a crucial role in cancer cell proliferation and metastasis. However, the mechanism underlying chemotherapeutic resistance in cancer cells has not been completely elucidated. The NHE1 inhibitor cariporide has been demonstrated to inhibit human cancer cell lines. The goal of this study was to provide new sights into improved cancer cell chemosensitivity mediated by cariporide with activation of the apoptosis pathway. Methods The NHE1 expression levels were first evaluated using the online database Oncomine and were determined by RT-PCR and western blot in vitro and in vivo. Cell proliferation was assessed In vitro through a CCK-8 assay, and apoptosis was analyzed by flow cytometry. An in vivo analysis was performed in BALB/c nude mice, which were intraperitoneally injected with MCF-7/ADR cells. Results NHE1 levels were significantly higher in breast cancer tissue than adjacent tissue, as well as in resistant cancer cells compared to sensitive cells. Cariporide induced the apoptosis of MCF-7/ADR cells and was associated with the intracellular accumulation of doxorubicin and G0/G1 cell cycle arrest. Moreover, cariporide decreased MDR1 expression and activated cleaved caspase-3 and caspase-9, promoting caspase-independent apoptosis in vitro. In vivo, cariporide significantly improved doxorubicin sensitivity in a xenograft model, enhancing tumor growth attenuation and diminishing tumor volume. Conclusions Our results demonstrate that cariporide significantly facilitates the sensitivity of breast cancer to doxorubicin both in vitro and in vivo. This finding suggests that NHE1 may be a novel adjuvant therapeutic candidate for the treatment of resistant breast cancer. Electronic supplementary material The online version of this article (10.1186/s12885-019-5397-7) contains supplementary material, which is available to authorized users.
- Published
- 2019
7. Clinicopathological features, surgical strategy and prognosis of duodenal gastrointestinal stromal tumors: a series of 300 patients
- Author
-
Fan Feng, Shushang Liu, Hongwei Zhang, Qiao Wang, Zhen Liu, Man Guo, Gaozan Zheng, Xiao Lian, Guanghui Xu, and Jinqiang Liu
- Subjects
Male ,Cancer Research ,medicine.medical_treatment ,Gastroenterology ,0302 clinical medicine ,Surgical oncology ,Duodenal Neoplasms ,Risk Factors ,Child ,Features ,Aged, 80 and over ,Incidence (epidemiology) ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Pancreaticoduodenectomy ,Prognosis ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Female ,Gastrointestinal stromal tumor ,Research Article ,Adult ,medicine.medical_specialty ,Mitotic index ,Adolescent ,Duodenum ,Gastrointestinal Stromal Tumors ,lcsh:RC254-282 ,Disease-Free Survival ,03 medical and health sciences ,Young Adult ,Stomach Neoplasms ,Internal medicine ,Genetics ,medicine ,Humans ,Risk factor ,neoplasms ,Duodenal Neoplasm ,Aged ,Retrospective Studies ,business.industry ,Retrospective cohort study ,digestive system diseases ,Surgery ,business - Abstract
Background The relatively low incidence of duodenal gastrointestinal stromal tumors (GISTs) and the unique anatomy make the surgical management and outcomes of this kind of tumor still under debate. Thus, this study aimed to explore the optimal surgical strategy and prognosis of duodenal GISTs. Methods A total of 300 cases of duodenal GISTs were obtained from our center (37 cases) and from case reports or series (263 cases) extracted from MEDLINE. Clinicopathological features, type of resections and survivals of duodenal GISTs were analyzed. Results The most common location of duodenal GISTs was descending portion (137/266, 51.5%). The median tumor size was 4 cm (0.1–28). Most patients (66.3%) received limited resection (LR). Pancreaticoduodenectomy (PD) was mainly performed for GISTs with larger tumor size or arose from descending portion (both P 0.05). Duodenal GISTs were significantly different from gastric GISTs with respect to tumor size, mitotic index and NIH risk category (all P
- Published
- 2018
8. Impact of body mass index on surgical outcomes of gastric cancer
- Author
-
Guanghui Xu, Xiao Lian, Zhen Liu, Hongwei Zhang, Fan Feng, Fei Wang, Qiao Wang, Man Guo, Gaozan Zheng, and Xiaohua Guo
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Complications ,Fever ,D2 gastrectomy ,Operative Time ,Blood Loss, Surgical ,lcsh:RC254-282 ,Gastroenterology ,Body Mass Index ,03 medical and health sciences ,Postoperative fever ,BMI ,Young Adult ,0302 clinical medicine ,Postoperative Complications ,Surgical oncology ,Gastrectomy ,Stomach Neoplasms ,Internal medicine ,Tumor stage ,Genetics ,medicine ,Humans ,Aged ,Aged, 80 and over ,Tumor size ,business.industry ,Cancer ,nutritional and metabolic diseases ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,Prognosis ,Survival Analysis ,Oncology ,030220 oncology & carcinogenesis ,Multivariate Analysis ,030211 gastroenterology & hepatology ,Female ,Lymph ,business ,Gastric cancer ,Body mass index ,Research Article - Abstract
Background The association between body mass index (BMI) and clinical outcomes of gastric cancer were still under debate. The aim of the present study was to investigate the impact of BMI on intraoperative conditions, postoperative complications and prognosis of gastric cancer. Methods From October 2008 to March 2015, 1210 gastric cancer patients treated with D2 gastrectomy were enrolled in the present study. Patients were divided into three groups: low BMI group (BMI 0.05). High BMI was associated with increased blood loss and operation time, and deceased number of retrieved lymph nodes (all P 0.05). Conclusions BMI was inversely associated with tumor size, tumor depth, LNM and tumor stage. High BMI was associated with increased blood loss and operation time, and deceased number of retrieved lymph nodes. Low BMI was associated with decreased rate of postoperative fever and decreased survival.
- Published
- 2018
9. Diagnostic and prognostic value of CEA, CA19–9, AFP and CA125 for early gastric cancer
- Author
-
Fan Feng, Xiao Lian, Guanghui Xu, Tian Yangzi, Daiming Fan, Shushang Liu, Hongwei Zhang, Zhen Liu, Man Guo, and Gaozan Zheng
- Subjects
0301 basic medicine ,Oncology ,Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Poor prognosis ,endocrine system diseases ,CA-19-9 Antigen ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,Carcinoembryonic antigen ,Antigen ,Surgical oncology ,Stomach Neoplasms ,Internal medicine ,Diagnosis ,Genetics ,medicine ,Biomarkers, Tumor ,Humans ,Tumor marker ,Risk factor ,Early Detection of Cancer ,Aged ,Early gastric cancer ,Aged, 80 and over ,biology ,business.industry ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Prognosis ,digestive system diseases ,Early Gastric Cancer ,Carcinoembryonic Antigen ,Survival Rate ,030104 developmental biology ,030220 oncology & carcinogenesis ,CA-125 Antigen ,biology.protein ,CA19-9 ,Female ,alpha-Fetoproteins ,business ,Research Article ,Follow-Up Studies - Abstract
Background The diagnostic and prognostic significance of carcinoembryonic antigen (CEA), carbohydrate associated antigen 19–9 (CA19–9), alpha-fetoprotein (AFP) and cancer antigen 125 (CA125) in early gastric cancer have not been investigated yet. Thus, the present study aimed to explore the diagnostic and prognostic significance of the four tumor markers for early gastric cancer. Methods From September 2008 to March 2015, 587 early gastric cancer patients were given radical gastrectomy in our center. The clinicopathological characteristics were recorded. The association between levels of CEA and CA19–9 and clinicopathological characteristics and prognosis of patients were analyzed. Results There were 444 men (75.6%) and 143 women (24.4%). The median age was 57 years (ranged 21–85). The 1-, 3- and 5-year overall survival rate was 99.1%, 96.8% and 93.1%, respectively. The positive rate of CEA, CA19–9, AFP and CA125 was 4.3%, 4.8%, 1.5% and 1.9%, respectively. The positive rate of all markers combined was 10.4%. The associations between the clinicopathological features and levels of CEA and CA19–9 were analyzed. No significant association was found between CEA level and clinicopathological features. However, elevated CA19–9 level was correlated with female gender and presence of lymph node metastasis. Age > 60 years old, presence of lymph node metastasis and elevation of CEA level were independent risk factors for poor prognosis of early gastric cancer. Conclusions The positive rates of CEA, CA19–9, APF and CA125 were relatively low for early gastric cancer. Elevation of CA19–9 level was associated with female gender and presence of lymph node metastasis. Elevation of CEA level was an independent risk factor for the poor prognosis of early gastric cancer.
- Published
- 2017
10. Clinicopathological features and prognosis of gastric cancer in young patients
- Author
-
Xiao Lian, Guanghui Xu, Tian Yangzi, Hongwei Zhang, Fan Feng, Daiming Fan, Lei Cai, Shushang Liu, Man Guo, and Zhen Liu
- Subjects
Oncology ,Curative resection ,Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Kaplan-Meier Estimate ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Age ,Surgical oncology ,Risk Factors ,Stomach Neoplasms ,Signet ring cell carcinoma ,Internal medicine ,Genetics ,medicine ,Humans ,Young adult ,Aged ,Proportional Hazards Models ,Retrospective Studies ,Aged, 80 and over ,business.industry ,Proportional hazards model ,digestive, oral, and skin physiology ,Cancer ,Retrospective cohort study ,Gastric cancer, Young, Clinicopathological features, Prognosis ,Middle Aged ,medicine.disease ,Adenocarcinoma, Mucinous ,030220 oncology & carcinogenesis ,Multivariate Analysis ,Clinicopathological features ,030211 gastroenterology & hepatology ,Female ,business ,Carcinoma, Signet Ring Cell ,Research Article - Abstract
Background The clinicopathological features and prognosis of gastric cancer in young patients are both limited and controversial. Therefore, the aim of this study was to define the clinicopathological features and prognosis of gastric cancer in young patients after curative resection. Methods From May 2008 to December 2014, 198 young patients (age ≤ 40 years) and 1096 middle-aged patients (55 ≤ age ≤ 64 years) were enrolled in this study. The clinicopathological features and prognosis of gastric cancer in these patients were analyzed. Results Compared with middle-aged patients, the proportion of females, lower third tumors, tumor size less than 5 cm, poorly differentiated tumors and T1 tumors were significantly higher in young patients (all P 0.05). The five-year overall survival rates were comparable between young patients and middle-aged patients (62.8 vs 54.7 %, P = 0.307). The tumor location, T status, N status and CA125 were independent predictors of prognosis in young patients. The overall survival of patients with tumors located in the upper or middle third was significantly lower than for those located in the lower third (60.8 vs 50.6 % vs 68.4 %, P = 0.016). The overall survival of CA125-positive patients was significantly lower than CA125-negative patients (49.0 vs 64.4 %, P = 0.001). Conclusion The clinicopathological features were significantly different between young and middle-aged patients. The prognosis of gastric cancer in young patients was equivalent to that of middle-aged patients. Tumor location, T status, N status and CA125 were independent risk factors for prognosis in young patients. Electronic supplementary material The online version of this article (doi:10.1186/s12885-016-2489-5) contains supplementary material, which is available to authorized users.
- Published
- 2016
11. Meta-analysis comparing laparoscopic versus open resection for gastric gastrointestinal stromal tumors larger than 5 cm.
- Author
-
Xiao Lian, Fan Feng, Man Guo, Lei Cai, Zhen Liu, Shushang Liu, Shuao Xiao, Gaozan Zheng, Guanghui Xu, Hongwei Zhang, Lian, Xiao, Feng, Fan, Guo, Man, Cai, Lei, Liu, Zhen, Liu, Shushang, Xiao, Shuao, Zheng, Gaozan, Xu, Guanghui, and Zhang, Hongwei
- Subjects
- *
GASTROINTESTINAL stromal tumors , *LAPAROSCOPIC surgery , *META-analysis , *STANDARD deviations ,TUMOR surgery - Abstract
Background: Data on the safety and feasibility of laparoscopic versus open resection for gastric gastrointestinal stromal tumors (GISTs) larger than 5 cm are limited. Therefore, the aim of this meta-analysis was to compared laparoscopic and open resection for gastric GISTs larger than 5 cm.Methods: We perform a literature search on PubMed, the Cochrane Library, and Embase. Review Manage version 5.1 (RevMan 5.1) was used for data analysis. The GRADE profiler software (version 3.6) was used to estimate the level of evidence.Results: A total of 6 observational studies and one unpublished retrospective cohort study met the inclusion criteria for the meta-analysis: 203 patients in LAP and 214 patients in OPEN group. The pooled result revealed that laparoscopic resection was associated with a same operative time (WMD = -0.87 min; 95% CI: -47.50 to 47.75; P = 0.97), intraoperative blood loss (WMD = -34.38 ml; 95% CI: -79.60 to 10.84; P = 0.14), overall complications (RR = 0.65; 95% CI: 0.38 to 1.12; P = 0.12), better 5-year disease-free survival (HR = 0.40; 95% CI: 0.17 to 0.91; P = 0.03) and overall survival (HR = 0.09; 95% CI: 0.02 to 0.40; P = 0.002) compared with open resection.Conclusion: Laparoscopic resection is a technically and oncologically safe and feasible approach for large-sized gastric GISTs (≥ 5 cm) compared to open resection. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
12. A novel chemotherapeutic sensitivity-testing system based on collagen gel droplet embedded 3D-culture methods for hepatocellular carcinoma.
- Author
-
Jun Hou, Zhixian Hong, Fan Feng, Yantao Chai, Yunkai Zhang, Qiyu Jiang, Yan Hu, Shunquan Wu, Yingsong Wu, Xunian Gao, Qiong Chen, Yong Wan, Jingfeng Bi, Zheng Zhang, Hou, Jun, Hong, Zhixian, Feng, Fan, Chai, Yantao, Zhang, Yunkai, and Jiang, Qiyu
- Subjects
LIVER cancer patients ,CANCER chemotherapy ,MULTIDRUG resistance ,ANTINEOPLASTIC agents ,OXALIPLATIN - Abstract
Background: Patients suffering from advanced stage hepatocellular carcinoma (HCC) often exhibit a poor prognosis or dismal clinical outcomes due to ineffective chemotherapy or a multi-drug resistance (MDR) process. Thus, it is urgent to develop a new chemotherapeutic sensitivity testing system for HCC treatment. The presence study investigated the potential application of a novel chemotherapeutic sensitivity-testing system based on a collagen gel droplet embedded 3D-culture system (CD-DST).Methods: Primary cells were separating from surgical resection specimens and then tested by CD-DST. To identify whether HCC cell lines or cells separating from clinical specimens contain MDR features, the cells were treated with an IC 50 (half maximal inhibitory concentration) or IC max (maximal inhibitory concentration) concentration of antitumor agents, e.g., 5-furuolouracil (5-FU), paclitaxel (PAC), cisplatin (CDDP), epirubicin (EPI), or oxaliplatin (L-OHP), and the inhibitory rates (IRs) were calculated.Results: HepG2 cells were sensitive to 5-FU, PAC, CDDP, EPI, or L-OHP; the IC 50 value is 0.83 ± 0.45 μg/ml, 0.03 ± 0.02 μg/ml, 1.15 ± 0.75 μg/ml, 0.09 ± 0.03 μg/ml, or 1.76 ± 0.44 μg/ml, respectively. Only eight (8/26), nine (9/26), or five (5/26) patients were sensitive to the IC max concentration of CDDP, EPI, or L-OHP; whereas only three (3/26), four (4/26), or two (2/26) patients were sensitive to the IC 50 concentration of CDDP, EPI, or L-OHP. No patients were sensitive to 5-FU or PAC.Conclusions: The in vitro drug sensitivity exanimation revealed the MDR features of HCC and examined the sensitivity of HCC cells from clinical specimens to anti-tumor agents. CD-DST may be a useful method to predict the potential clinical benefits of anticancer agents for HCC patients. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
13. Radiographic characteristics in congenital scoliosis associated with split cord malformation: a retrospective study of 266 surgical cases.
- Author
-
Fan Feng, Haining Tan, Xingye Li, Chong Chen, Zheng Li, Jianguo Zhang, Jianxiong Shen, Feng, Fan, Tan, Haining, Li, Xingye, Chen, Chong, Li, Zheng, Zhang, Jianguo, and Shen, Jianxiong
- Subjects
- *
SCOLIOSIS , *SPINAL cord abnormalities , *SYRINGOMYELIA , *RIB abnormalities , *PATIENTS - Abstract
Background: Vertebrae, ribs, and spinal cord are anatomically adjacent structures, and their close relationships are clinically important for planning better corrective surgical approach. The objective is to identify the radiographic characteristics in surgical patients with congenital scoliosis (CS) and coexisting split cord malformation (SCM).Methods: A total of 266 patients with CS and SCM underwent surgical treatment at our hospital between May 2000 and December 2015 was retrospectively identified. The demographic distribution and radiographic data were collected to investigate the characteristics of spine curve, vertebral, rib, and intraspinal anomalies. According to Pang's classification, all patients were divided into two groups: type I group is defined as two hemicords, each within a separate dural tube separated by a bony or cartilaginous medial spur, while type II group is defined as two hemicords within a single dural tube separated by a nonrigid fibrous septum.Results: There were 104 patients (39.1%) in Type I group and 162 patients (60.9%) in Type II group. SCM was most commonly found in the lower thoracic and lumbar regions. The mean length of the septum in Type I SCM was significantly shorter than Type II SCM (2.7 vs. 5.2 segments). Patients in Type I group had a higher proportion of kyphotic deformity (22.1%). The vertebral deformities were simple in only 16.5% and multiple in 83.5% of 266 cases. Patients in Type I group presented higher prevalence of multiple (90.4%) and extensive (5.1 segments) malformation of vertebrae. In addition, hypertrophic lamina and bulbous spinous processes were more frequent in Type I group (29.7%), even developing into the "volcano-shape" deformities. Rib anomalies occurred in 62.8% of all patients and 46.1% of them were complex anomalies. The overall prevalence of other intraspinal anomalies was 42.9%. The most common coexisting intraspinal anomalies was syringomyelia (30.5%).Conclusion: The current study, with the largest cohort to date, demonstrated that patients with CS and coexisting SCM presented high prevalence of multiple vertebral deformities, rib and intraspinal anomalies. The length of the split segment in Type I SCM was shorter than that in Type II SCM. Compared with Type II SCM, patients with Type I SCM presented with higher incidence of kyphotic deformity, more extensive and complicated vertebral anomalies, and more complex rib anomalies. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
14. Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China.
- Author
-
Lei Deng, Wei Li, Zhijun Zhong, Chao Gong, Xuehan Liu, Xiangming Huang, Li Xiao, Ruoxuan Zhao, Wuyou Wang, Fan Feng, Yue Zhang, Yanchun Hu, Hualin Fu, Min He, Kongju Wu, and Guangneng Peng
- Subjects
ZOONOSES ,GENOTYPES ,ENTEROCYTES ,MICROSATELLITE repeats ,HORSE diseases - Abstract
Background: Enterocytozoon bieneusi is one of the most prevalent causative species of diarrhea and enteric diseases in various hosts. E. bieneusi has been identified in humans, mammals, birds, rodents and reptiles in China, but few studies have reported E. bieneusi in horses. Therefore, the present study was conducted to assess the prevalence, molecular characteristics and zoonotic potential of E. bieneusi among horses in southwestern China. Findings: Three hundred and thirty-three fecal specimens were collected from horses on five farms in the Sichuan and Yunnan provinces of southwestern China. The prevalence of E. bieneusi was 22.5% (75/333), as determined by nested polymerase chain reaction and sequencing analysis of the internal transcribed spacer region of the ribosomal RNA gene of E. bieneusi. Altogether, 10 genotypes were identified among the 75 E. bieneusi-positive samples: four of these genotypes were known (horse1, horse2, SC02 and D) and six were novel (SCH1-4 and YNH1-2). Multilocus sequence typing using three microsatellites (MS1, MS3 and MS7) and one minisatellite (MS4) revealed three, two, three and three genotypes at these four loci, respectively. In phylogenetic analysis, all the genotypes of E. bieneusi obtained in this study were clustered into three distinct groups: D, SC02 and SCH1-3 were clustered into group 1 (zoonotic potential); SCH4 was clustered into group 2 (cattle-hosted); whereas horse2, YNH1 and YNH2 were clustered into group 6 (unclear zoonotic potential). Conclusions: This is the first report of E. bieneusi among horses in southwestern China. This is also the first multilocus genotyping analysis using microsatellite and minisatellite markers of E. bieneusi in horses. The presence of genotype D, which was previously identified in humans, and genotypes SC02 and SCH1-3, which belong to potential zoonotic group 1, these results indicate that horses are a potential source of human E. bieneusi infections in China. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
15. Clinicopathological features and prognosis of gastric cancer in young patients.
- Author
-
Shushang Liu, Fan Feng, Guanghui Xu, Zhen Liu, Yangzi Tian, Man Guo, Xiao Lian, Lei Cai, Daiming Fan, Hongwei Zhang, Liu, Shushang, Feng, Fan, Xu, Guanghui, Liu, Zhen, Tian, Yangzi, Guo, Man, Lian, Xiao, Cai, Lei, Fan, Daiming, and Zhang, Hongwei
- Subjects
- *
STOMACH cancer , *STOMACH cancer patients , *SURGICAL excision , *CARCINOEMBRYONIC antigen , *ALPHA fetoproteins , *CA 19-9 test , *PROGNOSIS , *ADENOCARCINOMA , *MULTIVARIATE analysis , *STOMACH tumors , *TUMORS , *PROPORTIONAL hazards models , *RETROSPECTIVE studies , *KAPLAN-Meier estimator - Abstract
Background: The clinicopathological features and prognosis of gastric cancer in young patients are both limited and controversial. Therefore, the aim of this study was to define the clinicopathological features and prognosis of gastric cancer in young patients after curative resection.Methods: From May 2008 to December 2014, 198 young patients (age ≤ 40 years) and 1096 middle-aged patients (55 ≤ age ≤ 64 years) were enrolled in this study. The clinicopathological features and prognosis of gastric cancer in these patients were analyzed.Results: Compared with middle-aged patients, the proportion of females, lower third tumors, tumor size less than 5 cm, poorly differentiated tumors and T1 tumors were significantly higher in young patients (all P < 0.05). The proportions of comorbidity, upper third tumors, well and moderately differentiated tumors, T4 tumors, and positive carcinoembryonic antigen (CEA), alpha fetoprotein (AFP) and carbohydrate antigen (CA) 19-9 were significantly lower in young patients (all P < 0.05). The distributions of N status and CA125 were comparable between young and middle-aged patients (all P > 0.05). The five-year overall survival rates were comparable between young patients and middle-aged patients (62.8 vs 54.7 %, P = 0.307). The tumor location, T status, N status and CA125 were independent predictors of prognosis in young patients. The overall survival of patients with tumors located in the upper or middle third was significantly lower than for those located in the lower third (60.8 vs 50.6 % vs 68.4 %, P = 0.016). The overall survival of CA125-positive patients was significantly lower than CA125-negative patients (49.0 vs 64.4 %, P = 0.001).Conclusion: The clinicopathological features were significantly different between young and middle-aged patients. The prognosis of gastric cancer in young patients was equivalent to that of middle-aged patients. Tumor location, T status, N status and CA125 were independent risk factors for prognosis in young patients. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
16. Estrogen receptor a enhances the transcriptional activity of ETS-1 and promotes the proliferation, migration and invasion of neuroblastoma cell in a ligand dependent manner.
- Author
-
Peng Cao, Fan Feng, Guofu Dong, Chunyong Yu, Sizhe Feng, Erlin Song, Guobing Shi, Yong Liang, and Guobiao Liang
- Subjects
- *
ESTROGEN receptors , *GENETIC transcription , *CANCER cell proliferation , *CANCER cell migration , *CANCER invasiveness , *NEUROBLASTOMA , *LIGANDS (Biochemistry) - Abstract
Background: It is well known that estrogen receptor a (ERa) participates in the pathogenic progress of breast cancer, hepatocellular carcinoma and head and neck squamous cell carcinoma. In neuroblastoma cells and related cancer clinical specimens, moreover, the ectopic expression of ERa has been identified. However, the detailed function of ERa in the proliferation of neuroblastoma cell is yet unclear. Methods: The transcriptional activity of ETS-1 (E26 transformation specific sequence 1) was measured by luciferase analysis. Western blot assays and Real-time RT-PCR were used to examine the expression of ERa, ETS-1 and its targeted genes. The protein-protein interaction between ERa and ETS-1 was determined by co-IP and GST-Pull down assays. The accumulation of ETS-1 in nuclear was detected by western blot assays, and the recruitment of ETS-1 to its targeted gene's promoter was tested by ChIP assays. Moreover, SH-SY5Y cells' proliferation, anchor-independent growth, migration and invasion were quantified using the MTT, soft agar or Trans-well assay, respectively. Results: The transcriptional activity of ETS-1 was significantly increased following estrogen treatment, and this effect was related to ligand-mediated activation of ERa. The interaction between the ERa and ETS-1 was identified, and enhancement of ERa activation would up-regulate the ETS-1 transcription factor activity via modulating its cytoplasm/nucleus translocation and the recruitment of ETS-1 to its target gene's promoter. Furthermore, treatment of estrogen increased proliferation, migration and invasion of neuroblastoma cells, whereas the antagonist of ERa reduced those effects. Conclusions: In this study, we provided evidences that activation of ERa promoted neuroblastoma cells proliferation and up-regulated the transcriptional activity of ETS-1. By investigating the role of ERa in the ETS-1 activity regulation, we demonstrated that ERa may be a novel ETS-1 co-activator and thus a potential therapeutic target in human neuroblastoma treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
17. Surgical correction of hyperlordosis in facioscapulohumeral muscular dystrophy: A case report.
- Author
-
Haining Tan, Fan Feng, Youxi Lin, Chong Chen, Zheng Li, Jianxiong Shen, Tan, Haining, Feng, Fan, Lin, Youxi, Chen, Chong, Li, Zheng, and Shen, Jianxiong
- Subjects
LORDOSIS ,FACIOSCAPULOHUMERAL muscular dystrophy ,BRACING (Structural engineering) ,SCOLIOSIS ,SURGERY ,PATIENTS ,THERAPEUTICS ,HEALTH surveys ,MUSCULAR dystrophy ,QUALITY of life ,QUESTIONNAIRES ,SPINAL fusion ,TREATMENT effectiveness - Abstract
Background: Hyperlordosis is common in facioscapulohumeral muscular dystrophy (FSHD), which cannot be controlled by bracing. While the surgical treatment is neither reported nor recommended in previous studies, we report the first corrective surgery for hyperlordosis in one wheelchair-dependent FSHD patient.Case Presentation: A 15-year-old, wheelchair-dependent girl complaining of hyperlordosis and lower extremity weakness was diagnosed as FSHD. Preoperative examination showed hyperlordosis of 116° with scoliosis of 44°. Posterior correction and bone graft fusion was performed. Postoperatively, the hyperlordosis was corrected to 72° and better sitting posture was gotten. 12 months' follow-up showed favorable correction with improvement in SF-36 and ODI score.Conclusions: The correction for hyperlordosis in FSHD is controversial. We report the first successful case of operative treatment by corrective spine surgery in these rare and demanding patient collective. Corrective surgery for spinal deformity, such as hyperlordosis and scoliosis, could do some help in posture and improve the quality of life especially in wheelchair-dependent patients. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
18. Surgical resection should be taken into consideration for the treatment of small gastric gastrointestinal stromal tumors.
- Author
-
Jianjun Yang, Fan Feng, Mengbin Li, Li Sun, Liu Hong, Lei Cai, Wenbin Wang, Guanghui Xu, and Hongwei Zhang
- Subjects
- *
GASTROINTESTINAL stromal tumors , *GASTROINTESTINAL tumors , *INTERSTITIAL cells , *RETROSPECTIVE studies - Abstract
Background The National Comprehensive Cancer Network (NCCN) recommends conservative follow-up for gastric gastrointestinal stromal tumors (GISTs) less than 2 cm. The aim of the present study was to investigate the clinical and pathological features of small gastric GISTs, reevaluate the risk potential, and discuss the treatment strategy of small gastric GISTs. Methods In this retrospective study, 63 cases of small gastric GISTs (less than 2 cm) were resected surgically from May 2010 to March 2013 in our department. Clinicopathological factors were collected and the malignant potential of small gastric GISTs was analyzed. Results The mitotic index of 14 out of 63 cases (22.22%) exceeded 5. The malignant potential of small gastric GISTs was related to tumor location (P = 0.0218). The mitotic index of 4 out of 8 GISTs (50%) located in gastric cardia exceeded 5, 8 out 28 GISTs (28.57%) located in the gastric fundus exceeded 5, and only 2 out of 27 GISTs (7.41%) located in the gastric body exceeded 5. We also discovered a good consistency between mitotic index and Ki-67 expression of small gastric GISTs. Conclusions Gastric GISTs less than 2 cm also have malignant potential. Thus, we recommended surgical resection of all small gastric GISTs once diagnosed. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
19. The transcription factor RBP-J-mediated signaling is essential for dendritic cells to evoke efficient anti-tumor immune responses in mice.
- Author
-
Fan Feng, Yao-Chun Wang, Xing-Bin Hu, Xiao-Wei Liu, Gang Ji, Yun-Ru Chen, Lin Wang, Fei He, Guo-Rui Dou, Liang Liang, Hong-Wei Zhang, and Hua Han
- Subjects
- *
DRUG resistance in cancer cells , *DENDRITIC cells , *LABORATORY mice , *IMMUNE response , *ANTIGENS , *LYMPH nodes - Abstract
Background: Dendritic cells (DCs) are professional antigen presenting cells that initiate specific immune responses against tumor cells. Transcription factor RBP-J-mediated Notch signaling regulates DC genesis, but whether this pathway regulates DC function in anti-tumor immunity remains unclear. In the present work we attempted to identify the role of Notch signaling in DC-mediated anti-tumor immune response. Results: When DCs were co-inoculated together with tumor cells, while the control DCs repressed tumor growth, the RBP-J deficient DCs had lost tumor repression activity. This was most likely due to that DCs with the conditionally ablated RBP-J were unable to evoke anti-tumor immune responses in the solid tumors. Indeed, tumors containing the RBP-J deficient DCs had fewer infiltrating T-cells, B-cells and NK-cells. Similarly, the draining lymph nodes of the tumors with RBP-J-/- DCs were smaller in size, and contained fewer cells of the T, B and NK lineages, as compared with the controls. At the molecular level, the RBP-J deficient DCs expressed lower MHC II, CD80, CD86, and CCR7, resulting in inefficient DC migration and T-cell activation in vitro and in vivo. T-cells stimulated by the RBP-J deficient DCs did not possess efficient cytotoxicity against tumor cells, in contrast to the control DCs. Conclusion: The RBP-J-mediated Notch signaling is essential for DC-dependent anti-tumor immune responses. The deficiency of RBP-J impairs the DC-based anti-tumor immunity through affecting series of processes including maturation, migration, antigen presentation and T-cell activation. The Notch signaling pathway might be a target for the establishment of the DC-based anti-tumor immunotherapies. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
20. Resolvin D1 reverses chronic pancreatitis-induced mechanical allodynia, phosphorylation of NMDA receptors, and cytokines expression in the thoracic spinal dorsal horn.
- Author
-
Quan-Xin F, Fan F, Xiang-Ying F, Shu-Jun L, Shi-Qi W, Zhao-Xu L, Xu-Jie Z, Qing-Chuan Z, and Wei W
- Subjects
- Abdominal Pain drug therapy, Abdominal Pain etiology, Animals, Anti-Inflammatory Agents pharmacology, Anxiety drug therapy, Docosahexaenoic Acids pharmacology, Exploratory Behavior drug effects, Hyperalgesia etiology, Hyperalgesia metabolism, Injections, Spinal, Interleukin-1beta metabolism, Interleukin-6 metabolism, Locomotion drug effects, Male, Pancreatitis, Chronic chemically induced, Pancreatitis, Chronic pathology, Phosphorylation, Posterior Horn Cells metabolism, Rats, Rats, Sprague-Dawley, Trinitrobenzenesulfonic Acid, Tumor Necrosis Factor-alpha metabolism, Anti-Inflammatory Agents therapeutic use, Docosahexaenoic Acids therapeutic use, Hyperalgesia drug therapy, Pancreatitis, Chronic complications, Receptors, N-Methyl-D-Aspartate metabolism
- Abstract
Background: We previously reported that immune activation in the spinal dorsal horn contributes to pain induced by chronic pancreatitis (CP). Targeting immune response in the CNS may provide effective treatments for CP-induced pain. Recent findings demonstrate that resolvin D1 (RvD1) can potently dampen inflammatory pain. We hypothesized that intrathecal injection of RvD1 may inhibit pain of CP., Methods: Rat CP model was built through intrapancreatic infusion of trinitrobenzene sulfonic acid (TNBS). All the rats were divided into three groups: TNBS, sham, and naïve controls and were further divided for intrathecal RvD1 administration. Pain behavior of rats was tested with von Frey filaments. Anxiety-like behavior and free locomotor and exploration of rats were evaluated by open field test and elevated plus maze. Pancreatic histology was evaluated with hematoxylin and eosin staining. Phosphorylation of NMDA receptor and expression of inflammatory cytokines were examined with Western blot, real-time RT-PCR and ELISA., Results: Behavioral study indicated that compared to the vehicle control, RvD1 (100 ng/kg) significantly decreased TNBS-induced mechanical allodynia at 2 h after administration (response frequencies: 49.2 ± 3.7% vs 71.3 ± 6.1%), and this effect was dose-dependent. Neither CP nor RvD1 treatment could affect anxiety-like behavior. CP or RvD1 treatment could not affect free locomotor and exploration of rats. Western blot analysis showed that compared with that of naïve group, phosphorylated NR1 (pNR1) and pNR2B in TNBS rats were significantly increased in the spinal cord (pNR1: 3.87±0.31 folds of naïve control, pNR2B: 4.17 ± 0.24 folds of naïve control). Compared to vehicle control, 10 ng/kg of RvD1 could significantly block expressions of pNR1 (2.21 ± 0.26 folds of naïve) and pNR2B (3.31 ± 0.34 folds of naïve). Real-time RT-PCR and ELISA data showed that RvD1 (10 ng/kg) but not vehicle could significantly block expressions of TNF-alpha, IL-1beta and IL-6. In addition, RvD1 did not influence pain behavior, NMDA receptor phosphorylation or cytokines production in sham-operated rats., Conclusions: These data highly suggest that RvD1 could be a novel and effective treatment for CP-induced chronic pain.
- Published
- 2012
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.