28 results on '"Esposito, Giovanni"'
Search Results
2. Incidence of new-onset hypertension before, during, and after the COVID-19 pandemic: a 7-year longitudinal cohort study in a large population
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Trimarco, Valentina, Izzo, Raffaele, Pacella, Daniela, Trama, Ugo, Manzi, Maria Virginia, Lombardi, Angela, Piccinocchi, Roberto, Gallo, Paola, Esposito, Giovanni, Piccinocchi, Gaetano, Lembo, Maria, Morisco, Carmine, Rozza, Francesco, Santulli, Gaetano, and Trimarco, Bruno
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- 2024
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3. Intranasal delivery of PEA-producing Lactobacillus paracasei F19 alleviates SARS-CoV-2 spike protein-induced lung injury in mice
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Del Re, Alessandro, Franzin, Silvia Basili, Lu, Jie, Palenca, Irene, Zilli, Aurora, Pepi, Federico, Troiani, Anna, Seguella, Luisa, Pesce, Marcella, Esposito, Giovanni, Sarnelli, Giovanni, and Esposito, Giuseppe
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- 2024
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4. Anti-VEGF therapy selects for clones resistant to glucose starvation in ovarian cancer xenografts
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Boso, Daniele, Tognon, Martina, Curtarello, Matteo, Minuzzo, Sonia, Piga, Ilaria, Brillo, Valentina, Lazzarini, Elisabetta, Carlet, Jessica, Marra, Ludovica, Trento, Chiara, Rasola, Andrea, Masgras, Ionica, Caporali, Leonardo, Del Ben, Fabio, Brisotto, Giulia, Turetta, Matteo, Pastorelli, Roberta, Brunelli, Laura, Navaglia, Filippo, Esposito, Giovanni, Grassi, Angela, and Indraccolo, Stefano
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- 2023
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5. An integrated modelling methodology for estimating global incidence and prevalence of hereditary spastic paraplegia subtypes SPG4, SPG7, SPG11, and SPG15
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Vander Stichele, Geert, Durr, Alexandra, Yoon, Grace, Schüle, Rebecca, Blackstone, Craig, Esposito, Giovanni, Buffel, Connor, Oliveira, Inês, Freitag, Christian, van Rooijen, Stephane, Hoffmann, Stéphanie, Thielemans, Leen, and Cowling, Belinda S.
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- 2022
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6. Impact of diabetes mellitus on clinical outcomes in patients affected by Covid-19
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Sardu, Celestino, Gargiulo, Giuseppe, Esposito, Giovanni, Paolisso, Giuseppe, and Marfella, Raffaele
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- 2020
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7. Adiponectin and insulin resistance are related to restenosis and overall new PCI in subjects with normal glucose tolerance: the prospective AIRE Study
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Sasso, Ferdinando Carlo, Pafundi, Pia Clara, Marfella, Raffaele, Calabrò, Paolo, Piscione, Federico, Furbatto, Fulvio, Esposito, Giovanni, Galiero, Raffaele, Gragnano, Felice, Rinaldi, Luca, Salvatore, Teresa, D’Amico, Michele, Adinolfi, Luigi Elio, and Sardu, Celestino
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- 2019
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8. Different age-independent effects of nutraceutical combinations on endothelium-mediated coronary flow reserve
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Esposito, Roberta, Sorrentino, Regina, Giugliano, Giuseppe, Avvedimento, Marisa, Paolillo, Roberta, Santoro, Ciro, Scalamogna, Maria, Esposito, Mafalda, Ilardi, Federica, Rozza, Francesco, Esposito, Giovanni, Galderisi, Maurizio, and Trimarco, Valentina
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- 2018
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9. Nutrient removal from high strength nitrate containing industrial wastewater using Chlorella sp. strain ACUF_802.
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Moreno Osorio, Jairo Hernan, Luongo, Vincenzo, Del Mondo, Angelo, Pinto, Gabriele, Pollio, Antonino, Frunzo, Luigi, Lens, Piet Nicolaas Luc, and Esposito, Giovanni
- Abstract
the research aim of this study was to characterize an isolated native strain of Chlorella sp. ACUF_802, well adapted to a high nitrate concentration environment and to investigate its potential to nitrate and phosphate removal from industrial wastewaters with the minimal addition of chemical reagents and energy. The isolated strain was identified and evaluated for its capability to support biomass growth and nutrient removal from synthetic wastewater in batch tests using different concentrations of carbon and nitrogen, different carbon sources and N:P ratios. The strain was isolated via the plating method from the settler of a pilot scale moving bed biofilm reactor performing a nitrification process. The strain was identified using molecular analysis with rDNA primers. Using sodium bicarbonate as carbon source, the batch productivity (71.43 mg L
−1 day−1 ) of the strain Chlorella sp. ACUF_802 was calculated with a logistic model and compared to the values reported in the literature. Assays on the effect of the N:P ratio indicated that the productivity was increased 36% when the N:P ratio was close to 1 (111.96 mg L−1 day−1 ), but for a complete phosphorus removal a 5:1 N:P ratio with nitrate concentrations ≤125 mg∙L−1 is recommended. The isolated microalgae strain Chlorella sp. ACUF_802 showed versatility to grow in the synthetic industrial wastewaters tested and can be considered as an appropriate organism for nitrogen removal from industrial wastewaters in the presence of an organic or inorganic carbon source. [ABSTRACT FROM AUTHOR]- Published
- 2018
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10. Diabetes does not impact the diagnostic performance of contrast-based fractional flow reserve: insights from the CONTRAST study.
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Gargiulo, Giuseppe, Stabile, Eugenio, Ferrone, Marco, Barbato, Emanuele, Zimmermann, Frederik M., Adjedj, Julien, Hennigan, Barry, Matsumura, Mitsuaki, Johnson, Nils P., Fearon, William F., Jeremias, Allen, Trimarco, Bruno, and Esposito, Giovanni
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DIABETES ,ADENOSINES ,CORONARY artery abnormalities ,RECEIVER operating characteristic curves ,SENSITIVITY & specificity (Statistics) ,CONTRAST analysis (Mathematical statistics) - Abstract
Background: Adenosine-free coronary pressure wire metrics have been proposed to test the functional significance of coronary artery lesions, but it is unexplored whether their diagnostic performance might be altered in patients with diabetes. Methods: We performed a post-hoc analysis of the CONTRAST study, which prospectively enrolled an international cohort of patients undergoing routine fractional flow reserve (FFR) assessment for standard indications. Paired, repeated measurements of all physiology metrics (Pd/Pa, iFR, contrast-based FFR, and FFR) were made. A central core laboratory analyzed blinded pressure tracings in a standardized fashion. Results: Of 763 subjects enrolled at 12 international centers, 219 (29%) had diabetes. The two groups were well-balanced for age, clinical presentation (stable or unstable), coronary vessel studied, volume and type of intracoronary contrast, and volume of intracoronary adenosine. A binary threshold of cFFR ≤ 0.83 produced an accuracy superior to both Pd/Pa and iFR when compared with FFR ≤ 0.80 in the absence of significant interaction with diabetes status; indeed, accuracy in subgroups of patients with or without diabetes was similar for cFFR (86.7 vs 85.4% respectively; p = 0.76), iFR (84.2 vs 80.0%, p = 0.29) and Pd/Pa (81.3 vs 78.9%, p = 0.55). There was no significant heterogeneity between patients with or without diabetes in terms of sensitivity and specificity of all metrics. The area under the receiver operating characteristic (ROC) curve was largest for cFFR compared with Pd/Pa and iFR which were equivalent (cFFR 0.961 and 0.928; Pd/Pa 0.916 and 0.870; iFR 0.911 and 0.861 in diabetic and non-diabetic patients respectively). Conclusions: cFFR provides superior diagnostic performance compared with Pd/Pa or iFR for predicting FFR irrespective of diabetes (clinicaltrials.gov identifier NCT02184117). [ABSTRACT FROM AUTHOR]
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- 2017
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11. Identification of the main determinants of abdominal aorta size: a screening by Pocket Size Imaging Device.
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Esposito, Roberta, Ilardi, Federica, Lomoriello, Vincenzo Schiano, Sorrentino, Regina, Sellitto, Vincenzo, Giugliano, Giuseppe, Esposito, Giovanni, Trimarco, Bruno, Galderisi, Maurizio, and Schiano Lomoriello, Vincenzo
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ABDOMINAL aorta ,DIAGNOSIS ,CORONARY disease ,ULTRASONIC imaging ,MEDICAL screening equipment ,CARDIOVASCULAR diseases risk factors ,ECHOCARDIOGRAPHY - Abstract
Background: Ultrasound exam as a screening test for abdominal aorta (AA) can visualize the aorta in 99% of patients and has a sensitivity and specificity approaching 100% in screening settings for aortic aneurysm. Pocket Size Imaging Device (PSID) has a potential value as a screening tool, because of its possible use in several clinical settings. Our aim was to assess the impact of demographics and cardiovascular (CV) risk factors on AA size by using PSID in an outpatient screening.Methods: Consecutive patients, referring for a CV assessment in a 6 months period, were screened. AA was visualized by subcostal view in longitudinal and transverse plans in order to determine the greatest anterior-posterior diameter. After excluding 5 patients with AA aneurysm, 508 outpatients were enrolled. All patients underwent a sequential assessment including clinical history with collection of CV risk factors, physical examination, PSID exam and standard Doppler echoc exam using a 2.5 transducer with harmonic capability, both by expert ultrasound operators, during the same morning. Standard echocardiography operators were blinded on PSID exam and viceversa.Results: Diagnostic accuracy of AA size by PSID was tested successfully with standard echo machine in a subgroup (n = 102) (rho = 0.966, p < 0.0001). AA diameter was larger in men than in women and in ≥50 -years old subjects than in those <50 -years old (both p < 0.0001). AA was larger in patients with coronary artery disease (CAD) (p < 0.0001). By a multivariate model, male sex (p < 0.0001), age and body mass index (both p < 0.0001), CAD (p < 0.01) and heart rate (p = 0.018) were independent predictors of AA size (cumulative R 2 = 0.184, p < 0.0001).Conclusion: PSID is a reliable tool for the screening of determinants of AA size. AA diameter is greater in men and strongly influenced by aging and overweight. CAD may be also associated to increased AA diameter. [ABSTRACT FROM AUTHOR]- Published
- 2017
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12. Genetic deletion in uncoupling protein 3 augments 18F-fluorodeoxyglucose cardiac uptake in the ischemic heart.
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Gargiulo, Sara, Petretta, Maria Piera, Greco, Adelaide, Panico, Mariarosaria, Larobina, Michele, Gramanzini, Matteo, Schiattarella, Gabriele G., Esposito, Giovanni, Petretta, Mario, Brunetti, Arturo, and Cuocolo, Alberto
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Background: We investigated the effects of uncoupling protein 3 (UCP3) genetic deletion on
18 F-fluorodeoxyglucose (FDG) cardiac uptake by positron emission tomography (PET)/computed tomography (CT) dedicated animal system after permanent coronary artery ligation. Methods: Cardiac18 F-FDG PET/CT was performed in UCP3 knockout (UCP3−/− ) and wild-type (WT) mice one week after induction of myocardial infarction or sham procedure. Results: In sham-operated mice no difference in left ventricular (LV) volume was detectable between WT and UCP3−/− . After myocardial infarction, LV volume was higher in both WT and UCP3−/− compared to sham animals, with a significant interaction (p < 0.05) between genotype and myocardial infarction. In sham-operated animals no difference in FDG standardized uptake value (SUV) was detectable between WT (1.8 ± 0.6) and UCP3−/− (1.8 ± 0.6). After myocardial infarction SUV was significantly higher in remote areas than in infarcted territories in both UCP3−/− and WT mice (both p < 0.01). Moreover, in remote areas, SUV was significantly higher (p < 0.001) in UCP3−/− as compared to WT, while in the infarcted territory SUV was comparable (p = 0.29). A significant relationship (r = 0.68, p < 0.001) between LV volume and SUV was found. Conclusions: In a mice model of permanent coronary occlusion, UCP3 deficiency results in a metabolic shift that favored glycolytic metabolism and increased FDG uptake in remote areas. [ABSTRACT FROM AUTHOR]- Published
- 2014
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13. Transradial approach for the endovascular treatment of type I endoleak after aortic aneurysm repair: a case report.
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Schiattarella, Gabriele Giacomo, Magliulo, Fabio, Laurino, Flora Ilaria, Bottino, Roberta, Bruno, Antonio Giulio, De Paulis, Michele, Sorropago, Antonio, Perrino, Cinzia, Amato, Bruno, Leosco, Dario, Trimarco, Bruno, and Esposito, Giovanni
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CORONARY disease ,DISEASES in men ,AORTIC aneurysm treatment ,THERAPEUTIC embolization ,SURGICAL excision - Abstract
Background: Endovascular repair of aortic aneurysms (EVAR) is obtained through the positioning of an aortic stent-graft, which excludes the aneurysmatic dilation. Type I endoleak is the most common complication, and it is caused by an incompetent proximal or distal attachment site, causing the separation between the stent-graft and the native arterial wall, and in turn creating direct communication between the aneurysm sac and the systemic arterial circulation. Endoleak occurrence is associated with high intrasac pressures, and requires a quick repair to prevent abdominal aortic aneurysm rupture. Case presentation: We report the first case of a 80-year-old man undergoing percutaneous closure of a peri-graft endoleak (type I) by transcatheter embolization through radial arterial access. Conclusion: The transradial approach has been shown to be a safe and effective alternative to the traditional transfemoral approach. A decrease in vascular complications and improved patient comfort are the primary benefits of this technique in patients with previous EVAR. [ABSTRACT FROM AUTHOR]
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- 2013
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14. Impact of microRNAs on regulatory networks and pathways in human colorectal carcinogenesis and development of metastasis.
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Pizzin, Silvia, Bisognin, Andrea, Mandruzzato, Susanna, Biasiolo, Marta, Facciolli, Arianna, Perill, Lisa, Rossi, Elisabetta, Esposito, Giovanni, Rugge, Massimo, Pilati, Pierluigi, Mocellin, Simone, Nitti, Donato, Bortoluzzi, Stefania, and Zanovello, Paola
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MICRORNA ,GENE expression ,GENE regulatory networks ,COLON cancer ,LIVER metastasis ,CANCER invasiveness - Abstract
Background: Qualitative alterations or abnormal expression of microRNAs (miRNAs) in colon cancer have mainly been demonstrated in primary tumors. Poorly overlapping sets of oncomiRs, tumor suppressor miRNAs and metastamiRs have been linked with distinct stages in the progression of colorectal cancer. To identify changes in both miRNA and gene expression levels among normal colon mucosa, primary tumor and liver metastasis samples, and to classify miRNAs into functional networks, in this work miRNA and gene expression profiles in 158 samples from 46 patients were analysed. Results: Most changes in miRNA and gene expression levels had already manifested in the primary tumors while these levels were almost stably maintained in the subsequent primary tumor-to-metastasis transition. In addition, comparing normal tissue, tumor and metastasis, we did not observe general impairment or any rise in miRNA biogenesis. While only few mRNAs were found to be differentially expressed between primary colorectal carcinoma and liver metastases, miRNA expression profiles can classify primary tumors and metastases well, including differential expression of miR-10b, miR-210 and miR-708. Of 82 miRNAs that were modulated during tumor progression, 22 were involved in EMT. qRT-PCR confirmed the down-regulation of miR-150 and miR-10b in both primary tumor and metastasis compared to normal mucosa and of miR-146a in metastases compared to primary tumor. The upregulation of miR-201 in metastasis compared both with normal and primary tumour was also confirmed. A preliminary survival analysis considering differentially expressed miRNAs suggested a possible link between miR-10b expression in metastasis and patient survival. By integrating miRNA and target gene expression data, we identified a combination of interconnected miRNAs, which are organized into sub-networks, including several regulatory relationships with differentially expressed genes. Key regulatory interactions were validated experimentally. Specific mixed circuits involving miRNAs and transcription factors were identified and deserve further investigation. The suppressor activity of miR-182 on ENTPD5 gene was identified for the first time and confirmed in an independent set of samples. Conclusions: Using a large dataset of CRC miRNA and gene expression profiles, we describe the interplay of miRNA groups in regulating gene expression, which in turn affects modulated pathways that are important for tumor development. [ABSTRACT FROM AUTHOR]
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- 2013
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15. Abdominal aortic aneurysm in patients affected by intermittent claudication: prevalence and clinical predictors.
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Giugliano, Giuseppe, Laurenzano, Eugenio, Rengo, Carlo, De Rosa, Giovanna, Brevetti, Linda, Sannino, Anna, Perrino, Cinzia, Chiariotti, Lorenzo, Schiattarella, Gabriele Giacomo, Serino, Federica, Ferrone, Marco, Scudiero, Fernando, Carbone, Andreina, Sorropago, Antonio, Amato, Bruno, Trimarco, Bruno, and Esposito, Giovanni
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AORTIC aneurysms ,DISEASES in older people ,ARTERIAL diseases ,COHORT analysis ,INTERMITTENT claudication ,DISEASE prevalence ,NEUTROPHILS ,PATIENTS - Abstract
Background: Abdominal aortic aneurysm (AAA) is a frequent cause of death among elderly. Patients affected by lower extremity peripheral arterial disease (LE-PAD) seem to be particularly at high risk for AAA. We aimed this study at assessing the prevalence and the clinical predictors of the presence of AAA in a homogeneous cohort of LE-PAD patients affected by intermittent claudication. Methods: We performed an abdominal ultrasound in 213 consecutive patients with documented LE-PAD (ankle/ brachial index ⩽0.90) attending our outpatient clinic for intermittent claudication. For each patient we registered cardiovascular risk factors and comorbidities, and measured neutrophil count. Results: The ultrasound was inconclusive in 3 patients (1.4%), thus 210 patients (169 males, 41 females, mean age 65.9 ± 9.8 yr) entered the study. Overall, AAA was present in 19 patients (9.0%), with a not significant higher prevalence in men than in women (10.1% vs 4.9%, p = 0.300). Patients with AAA were older (71.2 ± 7.0 vs 65.4 ± 9.9 years, p = 0.015), were more likely to have hypertension (94.7% vs 71.2%, p = 0.027), and greater neutrophil count (5.5 [4.5 - 6.2] vs 4.1 [3.2 - 5.5] x10
3 /μL, p = 0.010). Importantly, the c-statistic for neutrophil count (0.73, 95% CI 0.60 - 0.86, p =0.010) was higher than that for age (0.67, CI 0.56-0.78, p = 0.017). The prevalence of AAA in claudicant patients with a neutrophil count ⩾ 5.1 x103 /μL (cut-off identified at ROC analysis) was as high as 29.0%. Conclusions: Prevalence of AAA in claudicant patients is much higher than that reported in the general population. Ultrasound screening should be considered in these patients, especially in those with an elevated neutrophil count. [ABSTRACT FROM AUTHOR]- Published
- 2012
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16. Endovascular treatment of lower extremity arteries is associated with an improved outcome in diabetic patients affected by intermittent claudication.
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Giugliano, Giuseppe, Perrino, Cinzia, Schiano, Vittorio, Brevetti, Linda, Sannino, Anna, Schiattarella, Gabriele Giacomo, Gargiulo, Giuseppe, Serino, Federica, Ferrone, Marco, Scudiero, Fernando, Carbone, Andreina, Bruno, Antonio, Amato, Bruno, Trimarco, Bruno, and Esposito, Giovanni
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LEG diseases ,ARTERIAL diseases ,REVASCULARIZATION (Surgery) ,HEALTH outcome assessment ,TRANSLUMINAL angioplasty ,INTERMITTENT claudication treatment ,MYOCARDIAL infarction risk factors ,DISEASE risk factors - Abstract
Background: Lower extremity peripheral arterial disease (LE-PAD) is a highly prevalent condition among diabetic patients, associated with reduced walking capacity and a high incidence of cardiovascular events. Endovascular revascularization of lower extremities arteries improves walking performance and quality of life of diabetic patients affected by intermittent claudication, but few studies evaluated the impact of revascularization on cardiovascular outcome in this high-risk population. Accordingly, in the present study we evaluated if leg-ischemia resolution by effective lower limbs percutaneous revascularization can also impact cardiovascular outcome in a homogeneous group of diabetic patients affected by intermittent claudication. Methods: 236 diabetic patients affected by LE-PAD at stage II of Fontaine's classification, with ankle/brachial index ⩽0.90 and one or more hemodynamically significant stenosis in at least one artery of the ileo-femoro-popliteal axis were enrolled in the study. According to the Trans-Atlantic Inter Society Consensus II recommendations, 123 (52.1%) underwent percutaneous transluminal angioplasty (PTA group), while 113 (47.9%) underwent conservative medical therapy only (MT group). The incidence of major cardiovascular events (cardiovascular death, myocardial infarction, ischemic stroke, coronary or carotid revascularization) was prospectively analyzed with Kaplan-Meier curves and the risk of developing a cardiovascular event calculated by Cox analyses. Results: No baseline difference in cardiovascular risk factors were observed between the PTA and MT groups, except for a lower prevalence of males in PTA group (74.8% vs. 85.8%, p=0.034). Furthermore, patients in the PTA group showed a worse walking capacity as expressed by maximum walking distance (108.7 ± 300.9 vs 378.4 ± 552.3 meters, p<0.001). During a median follow-up of 20 months (12.0-29.0), the incidence of cardiovascular events was markedly lower in patients in the PTA group with respect to patients in the MT group (7.3% vs. 22.1%, p=0.001), and patients of the MT group had at Cox analysis a 3.9 increased risk with respect to PTA group, after adjustment for potential confounding factors (95% CI 1.1-15.3, p=0.049). Conclusions: The present study shows that lower limbs revascularization of diabetic patients affected by intermittent claudication, in addition to improve walking performance, is associated with a reduction in the incidence of future major cardiovascular events. [ABSTRACT FROM AUTHOR]
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- 2012
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17. Ankle/brachial index to everyone.
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Giugliano, Giuseppe, Sannino, Anna, Brevetti, Linda, Perrino, Cinzia, Schiattarella, Gabriele Giacomo, Franzone, Anna, Serino, Federica, Ferrone, Marco, Scudiero, Fernando, Carbone, Andreina, De Paulis, Michele, Izzo, Raffaele, Amato, Bruno, Trimarco, Bruno, and Esposito, Giovanni
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CARDIOVASCULAR diseases risk factors ,ATHEROSCLEROSIS ,LEG diseases ,ARTERIAL diseases ,ISCHEMIA ,BRACHIAL artery ,PATIENTS ,PHYSIOLOGY ,DISEASE risk factors - Abstract
Background: In the last years significant attention has been paid in identifying markers of subclinical atherosclerosis or of increased cardiovascular risk. Method: An abnormal ankle/brachial index (ABI) identifies patients affected by lower extremity peripheral arterial disease, and even more important, represents a powerful predictor of the development of future ischemic cardiovascular events. Conclusions: In our opinion, ABI is a cardiovascular risk prediction tool with very desirable properties that might become a routine measurement in clinical practice. [ABSTRACT FROM AUTHOR]
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- 2012
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18. The role of atherectomy in the treatment of lower extremity peripheral artery disease.
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Franzone, Anna, Ferrone, Marco, Carotenuto, Giuseppe, Carbone, Andreina, Scudiero, Laura, Serino, Federica, Scudiero, Fernando, Izzo, Raffaele, Piccolo, Raffaele, Saviano, Savio, Amato, Bruno, Perrino, Cinzia, Trimarco, Bruno, and Esposito, Giovanni
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REVASCULARIZATION (Surgery) ,TRANSLUMINAL angioplasty ,ENDARTERECTOMY ,CORONARY restenosis ,VASCULAR surgery ,LEG diseases ,DISEASE risk factors - Abstract
Background: The incidence of lower extremity peripheral artery disease (LE-PAD) continues to increase and associated morbidity remains high. Despite the significant development of percutaneous revascularization strategies, over the past decade, LE-PAD still represents a unique challenge for interventional cardiologists and vascular surgeons. Method: Typical features of atherosclerosis that affects peripheral vascular bed (diffuse nature, poor distal runoff, critical limb ischemia, chronic total occlusion) contribute to the disappointing results of traditional percutaneous transluminal angioplasty (PTA). New technologies have been developed in attempt to improve the safety and effectiveness of percutaneous revascularization. Among these, atherectomy, debulking and removing atherosclerotic plaque, offers the potential advantage of eliminating stretch on arterial walls and reducing rates of restenosis. Conclusions: This review summarizes the features and the current applications of new debulking devices. [ABSTRACT FROM AUTHOR]
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- 2012
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19. The G Protein regulators EGL-10 and EAT-16, the Giα GOA-1 and the Gqα EGL-30 modulate the response of the C. elegans ASH polymodal nociceptive sensory neurons to repellents.
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Esposito, Giovanni, Amoroso, Maria R., Bergamasco, Carmela, Di Schiavi, Elia, and Bazzicalupo, Paolo
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G proteins , *SENSORY receptors , *MEMBRANE proteins , *NOCICEPTORS , *CONDITIONED response - Abstract
Background: Polymodal, nociceptive sensory neurons are key cellular elements of the way animals sense aversive and painful stimuli. In Caenorhabditis elegans, the polymodal nociceptive ASH sensory neurons detect aversive stimuli and release glutamate to generate avoidance responses. They are thus useful models for the nociceptive neurons of mammals. While several molecules affecting signal generation and transduction in ASH have been identified, less is known about transmission of the signal from ASH to downstream neurons and about the molecules involved in its modulation. Results: We discovered that the regulator of G protein signalling (RGS) protein, EGL-10, is required for appropriate avoidance responses to noxious stimuli sensed by ASH. As it does for other behaviours in which it is also involved, egl-10 interacts genetically with the Go/iα protein GOA-1, the Gqa protein EGL-30 and the RGS EAT-16. Genetic, behavioural and Ca2+ imaging analyses of ASH neurons in live animals demonstrate that, within ASH, EGL-10 and GOA-1 act downstream of stimulus-evoked signal transduction and of the main transduction channel OSM-9. EGL-30 instead appears to act upstream by regulating Ca2+ transients in response to aversive stimuli. Analysis of the delay in the avoidance response, of the frequency of spontaneous inversions and of the genetic interaction with the diacylglycerol kinase gene, dgk-1, indicate that EGL-10 and GOA-1 do not affect signal transduction and neuronal depolarization in response to aversive stimuli but act in ASH to modulate downstream transmission of the signal. Conclusions: The ASH polymodal nociceptive sensory neurons can be modulated not only in their capacity to detect stimuli but also in the efficiency with which they respond to them. The Gα and RGS molecules studied in this work are conserved in evolution and, for each of them, mammalian orthologs can be identified. The discovery of their role in the modulation of signal transduction and signal transmission of nociceptors may help us to understand how pain is generated and how its control can go astray (such as chronic pain) and may suggest new pain control therapies. [ABSTRACT FROM AUTHOR]
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- 2010
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20. Use of statins in lower extremity artery disease: a review.
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Gargiulo, Giuseppe, Giugliano, Giuseppe, Brevetti, Linda, Sannino, Anna, Schiattarella, Gabriele Giacomo, Serino, Federica, Carbone, Andreina, Scudiero, Fernando, Ferrone, Marco, Corrado, Roberto, Izzo, Raffaele, Chiariotti, Lorenzo, Perrino, Cinzia, Amato, Bruno, Trimarco, Bruno, and Esposito, Giovanni
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ARTERIAL diseases ,ETIOLOGY of atherosclerosis ,TREATMENT of diseases in older people ,AGE factors in cardiovascular disease ,STATINS (Cardiovascular agents) ,LOW density lipoproteins ,QUALITY of life - Abstract
Background: Lower extremity artery disease (LE-PAD) is one of the most common manifestations of atherosclerosis, particularly in elderly patients, and it is related to a high cardiovascular risk. Description: It is well established that statin therapy is characterized by crucial benefits on cardiovascular system by limiting atherosclerotic progression and reducing cardiovascular events and mortality. A growing body of evidence support efficacy of statins in LE-PAD due to the ability of both reducing cardiovascular risk and improving walking distance and, hence, quality of life. Consequently, statin therapy should be considered in all LE-PAD patients and new LDL-cholesterol targets should be reached. Conclusions: Our opinion is that statin therapy remains still underutilized or with inadequate dosage, so therapy of LE-PAD patients should be improved to obtain all the demonstrated benefits of statins. [ABSTRACT FROM AUTHOR]
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- 2012
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21. Genetic deletion in uncoupling protein 3 augments 18F-fluorodeoxyglucose cardiac uptake in the ischemic heart.
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Gargiulo, Sara, Petretta, Maria Piera, Greco, Adelaide, Panico, Mariarosaria, Larobina, Michele, Gramanzini, Matteo, Schiattarella, Gabriele G, Esposito, Giovanni, Petretta, Mario, Brunetti, Arturo, and Cuocolo, Alberto
- Abstract
Background: We investigated the effects of uncoupling protein 3 (UCP3) genetic deletion on 18F-fluorodeoxyglucose (FDG) cardiac uptake by positron emission tomography (PET)/computed tomography (CT) dedicated animal system after permanent coronary artery ligation.Methods: Cardiac 18F-FDG PET/CT was performed in UCP3 knockout (UCP3-/-) and wild-type (WT) mice one week after induction of myocardial infarction or sham procedure.Results: In sham-operated mice no difference in left ventricular (LV) volume was detectable between WT and UCP3-/-. After myocardial infarction, LV volume was higher in both WT and UCP3-/- compared to sham animals, with a significant interaction (p < 0.05) between genotype and myocardial infarction. In sham-operated animals no difference in FDG standardized uptake value (SUV) was detectable between WT (1.8 ± 0.6) and UCP3-/- (1.8 ± 0.6). After myocardial infarction SUV was significantly higher in remote areas than in infarcted territories in both UCP3-/- and WT mice (both p < 0.01). Moreover, in remote areas, SUV was significantly higher (p < 0.001) in UCP3-/- as compared to WT, while in the infarcted territory SUV was comparable (p = 0.29). A significant relationship (r = 0.68, p < 0.001) between LV volume and SUV was found.Conclusions: In a mice model of permanent coronary occlusion, UCP3 deficiency results in a metabolic shift that favored glycolytic metabolism and increased FDG uptake in remote areas. [ABSTRACT FROM AUTHOR]- Published
- 2014
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22. Different age-independent effects of nutraceutical combinations on endothelium-mediated coronary flow reserve
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Roberta Esposito, Giuseppe Giugliano, Ciro Santoro, Roberta Paolillo, Giovanni Esposito, Valentina Trimarco, Mafalda Esposito, Maurizio Galderisi, Maria Scalamogna, Marisa Avvedimento, Francesco Rozza, Regina Sorrentino, Federica Ilardi, Esposito, Roberta, Sorrentino, Regina, Giugliano, Giuseppe, Avvedimento, Marisa, Paolillo, Roberta, Santoro, Ciro, Scalamogna, Maria, Esposito, Mafalda, Ilardi, Federica, Rozza, Francesco, Esposito, Giovanni, Galderisi, Maurizio, and Trimarco, Valentina
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0301 basic medicine ,Nut ,lcsh:Immunologic diseases. Allergy ,Aging ,medicine.medical_specialty ,Coronary flow reserve ,Immunology ,030204 cardiovascular system & hematology ,lcsh:Geriatrics ,Gastroenterology ,Coronary artery disease ,03 medical and health sciences ,Cholesterol-lowering ,0302 clinical medicine ,Internal medicine ,Cold pressure test ,medicine ,Red yeast rice ,Policosanol ,medicine.diagnostic_test ,business.industry ,Research ,digestive, oral, and skin physiology ,food and beverages ,Nutraceutical therapy ,Endothelial function ,medicine.disease ,lcsh:RC952-954.6 ,030104 developmental biology ,Blood pressure ,Lipid profile ,business ,lcsh:RC581-607 ,Blood sampling ,medicine.drug - Abstract
Some components of Nutraceuticals (NUT) such as red yeast rice and Morus alba have demonstrated positive effects on the endothelial function in hypercholesterolemic subjects. Our aim was to compare the effects of two different NUT combinations on cold pressure test (CPT) derived coronary flow reserve (CFR) assessed by transthoracic echo-Doppler. In a randomized, single-blind study, 28 consecutive patients with a variety of cardiovascular risk factors received NUT A (LopiGLIK®: berberine, red yeast rice powder, and leaf extract of Morus alba) or B (Armolipid Plus®: policosanol, red yeast rice, berberine, astaxantine, folic acidandcoenzyme Q10). An echo-Doppler exam with evaluation of CFR was performed at baseline, 2 h (acute test) and 30 days after daily NUT assumption. Blood sampling for metabolic profile and platelet aggregometry was performed at baseline and after 30 days of daily NUT assumption. CFR was not significantly modified at the acute test. After 30 days, CFR improved with NUT A (p
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- 2018
23. Diabetes does not impact the diagnostic performance of contrast-based fractional flow reserve: insights from the CONTRAST study
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Frederik M. Zimmermann, Bruno Trimarco, Eugenio Stabile, William F. Fearon, Giuseppe Gargiulo, Marco Ferrone, Giovanni Esposito, Emanuele Barbato, Julien Adjedj, Mitsuaki Matsumura, Nils P. Johnson, Allen Jeremias, Barry Hennigan, Gargiulo, Giuseppe, Stabile, Eugenio, Ferrone, Marco, Barbato, Emanuele, Zimmermann, Frederik M, Adjedj, Julien, Hennigan, Barry, Matsumura, Mitsuaki, Johnson, Nils P, Fearon, William F, Jeremias, Allen, Trimarco, Bruno, and Esposito, Giovanni
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Male ,Contrast medium ,Instantaneous wave-free ratio ,medicine.medical_specialty ,Internationality ,Adenosine ,Endocrinology, Diabetes and Metabolism ,Contrast Media ,Hyperemia ,Fractional flow reserve ,030204 cardiovascular system & hematology ,Diabete ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Internal medicine ,medicine ,Diabetes Mellitus ,Humans ,030212 general & internal medicine ,Prospective Studies ,Prospective cohort study ,Angiology ,Aged ,Original Investigation ,Receiver operating characteristic ,business.industry ,Diabetes ,Middle Aged ,medicine.disease ,Fractional Flow Reserve, Myocardial ,Coronary vessel ,Cardiology ,Female ,business ,Cardiology and Cardiovascular Medicine ,Coronary lesion ,Resting metrics - Abstract
Background Adenosine-free coronary pressure wire metrics have been proposed to test the functional significance of coronary artery lesions, but it is unexplored whether their diagnostic performance might be altered in patients with diabetes. Methods We performed a post-hoc analysis of the CONTRAST study, which prospectively enrolled an international cohort of patients undergoing routine fractional flow reserve (FFR) assessment for standard indications. Paired, repeated measurements of all physiology metrics (Pd/Pa, iFR, contrast-based FFR, and FFR) were made. A central core laboratory analyzed blinded pressure tracings in a standardized fashion. Results Of 763 subjects enrolled at 12 international centers, 219 (29%) had diabetes. The two groups were well-balanced for age, clinical presentation (stable or unstable), coronary vessel studied, volume and type of intracoronary contrast, and volume of intracoronary adenosine. A binary threshold of cFFR ≤ 0.83 produced an accuracy superior to both Pd/Pa and iFR when compared with FFR ≤ 0.80 in the absence of significant interaction with diabetes status; indeed, accuracy in subgroups of patients with or without diabetes was similar for cFFR (86.7 vs 85.4% respectively; p = 0.76), iFR (84.2 vs 80.0%, p = 0.29) and Pd/Pa (81.3 vs 78.9%, p = 0.55). There was no significant heterogeneity between patients with or without diabetes in terms of sensitivity and specificity of all metrics. The area under the receiver operating characteristic (ROC) curve was largest for cFFR compared with Pd/Pa and iFR which were equivalent (cFFR 0.961 and 0.928; Pd/Pa 0.916 and 0.870; iFR 0.911 and 0.861 in diabetic and non-diabetic patients respectively). Conclusions cFFR provides superior diagnostic performance compared with Pd/Pa or iFR for predicting FFR irrespective of diabetes (clinicaltrials.gov identifier NCT02184117).
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- 2017
24. Transradial approach for the endovascular treatment of type I endoleak after aortic aneurysm repair: a case report
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Roberta Bottino, Giovanni Esposito, Fabio Magliulo, Antonio Bruno, Cinzia Perrino, Flora Ilaria Laurino, Bruno Trimarco, Dario Leosco, Antonio Sorropago, Gabriele G. Schiattarella, Michele De Paulis, Bruno Amato, Gabriele, Schiattarella, Fabio, Magliulo, Flora, Laurino, Roberta, Bottino, Antonio, Bruno, Michele De, Pauli, Antonio, Sorropago, Perrino, Cinzia, Amato, Bruno, Leosco, Dario, Trimarco, Bruno, Esposito, Giovanni, Schiattarella, Gabriele Giacomo, Magliulo, Fabio, Laurino, Flora Ilaria, Bottino, Roberta, Bruno, Antonio Giulio, De Paulis, Michele, and Sorropago, Antonio
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Male ,medicine.medical_specialty ,Percutaneous ,Endoleak ,Aneurysm ,medicine.artery ,Medicine ,Humans ,cardiovascular diseases ,Aortic Abdominal Aneurysm Radial Artery Aortic Abdominal Aneurysm Percutaneous Closure Radial Access ,Radial artery ,Endovascular treatment ,Patient comfort ,Aged, 80 and over ,Aortic aneurysm repair ,business.industry ,Endovascular Procedures ,General Medicine ,medicine.disease ,Abdominal aortic aneurysm ,Surgery ,surgical procedures, operative ,Cardiovascular and Metabolic Diseases ,Radial Artery ,cardiovascular system ,Radiology ,business ,Complication ,Research Article ,Aortic Aneurysm, Abdominal - Abstract
BACKGROUND: Endovascular repair of aortic aneurysms (EVAR) is obtained through the positioning of an aortic stent-graft, which excludes the aneurysmatic dilation. Type I endoleak is the most common complication, and it is caused by an incompetent proximal or distal attachment site, causing the separation between the stent-graft and the native arterial wall, and in turn creating direct communication between the aneurysm sac and the systemic arterial circulation. Endoleak occurrence is associated with high intrasac pressures, and requires a quick repair to prevent abdominal aortic aneurysm rupture. CASE PRESENTATION: We report the first case of a 80-year-old man undergoing percutaneous closure of a peri-graft endoleak (type I) by transcatheter embolization through radial arterial access. CONCLUSION: The transradial approach has been shown to be a safe and effective alternative to the traditional transfemoral approach. A decrease in vascular complications and improved patient comfort are the primary benefits of this technique in patients with previous EVAR.
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- 2013
25. Endovascular treatment of lower extremity arteries is associated with an improved outcome in diabetic patients affected by intermittent claudication
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Vittorio Schiano, Gabriele G. Schiattarella, Giovanni Esposito, Fernando Scudiero, Federica Serino, Bruno Trimarco, Cinzia Perrino, Bruno Amato, Linda Brevetti, Anna Sannino, Andreina Carbone, Antonio Bruno, Marco Ferrone, Giuseppe Giugliano, Giuseppe Gargiulo, Giugliano, Giuseppe, Perrino, Cinzia, Schiano, V., Brevetti, L., Sannino, A., Schiattarella, G. G., Gargiulo, G., Serino, F., Ferrone, M., Scudiero, F., Carbone, A., Bruno, A., Amato, Bruno, Trimarco, Bruno, Esposito, Giovanni, Giugliano G., Perrino C., Schiano V., Brevetti L., Sannino A., Schiattarella G.G., Gargiulo G., Serino F., Ferrone M., Scudiero F., Carbone A., Bruno A., Amato B., Trimarco B., and Esposito G.
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Carotid Artery Diseases ,Male ,medicine.medical_treatment ,Myocardial Infarction ,Coronary Artery Disease ,Kaplan-Meier Estimate ,Walking ,Coronary artery disease ,Prospective Studies ,Stroke ,education.field_of_study ,General Medicine ,Middle Aged ,Treatment Outcome ,Lower Extremity ,Cardiology ,Female ,medicine.symptom ,Human ,Research Article ,medicine.medical_specialty ,Diabetic Angiopathie ,Population ,lcsh:Surgery ,Diabetic angiopathy ,Revascularization ,Follow-Up Studie ,Peripheral Arterial Disease ,Internal medicine ,Angioplasty ,medicine ,Humans ,education ,Aged ,Proportional Hazards Models ,Carotid Artery Disease ,business.industry ,lcsh:RD1-811 ,Critical limb ischemia ,Intermittent Claudication ,medicine.disease ,Intermittent claudication ,Surgery ,Prospective Studie ,Death, Sudden, Cardiac ,Cardiovascular and Metabolic Diseases ,Proportional Hazards Model ,business ,Diabetic Angiopathies ,Follow-Up Studies - Abstract
Background Lower extremity peripheral arterial disease (LE-PAD) is a highly prevalent condition among diabetic patients, associated with reduced walking capacity and a high incidence of cardiovascular events. Endovascular revascularization of lower extremities arteries improves walking performance and quality of life of diabetic patients affected by intermittent claudication, but few studies evaluated the impact of revascularization on cardiovascular outcome in this high-risk population. Accordingly, in the present study we evaluated if leg-ischemia resolution by effective lower limbs percutaneous revascularization can also impact cardiovascular outcome in a homogeneous group of diabetic patients affected by intermittent claudication. Methods 236 diabetic patients affected by LE-PAD at stage II of Fontaine’s classification, with ankle/brachial index ≤0.90 and one or more hemodynamically significant stenosis in at least one artery of the ileo-femoro-popliteal axis were enrolled in the study. According to the Trans-Atlantic Inter Society Consensus II recommendations, 123 (52.1%) underwent percutaneous transluminal angioplasty (PTA group), while 113 (47.9%) underwent conservative medical therapy only (MT group). The incidence of major cardiovascular events (cardiovascular death, myocardial infarction, ischemic stroke, coronary or carotid revascularization) was prospectively analyzed with Kaplan-Meier curves and the risk of developing a cardiovascular event calculated by Cox analyses. Results No baseline difference in cardiovascular risk factors were observed between the PTA and MT groups, except for a lower prevalence of males in PTA group (74.8% vs. 85.8%, p=0.034). Furthermore, patients in the PTA group showed a worse walking capacity as expressed by maximum walking distance (108.7 ± 300.9 vs 378.4 ± 552.3 meters, p Conclusions The present study shows that lower limbs revascularization of diabetic patients affected by intermittent claudication, in addition to improve walking performance, is associated with a reduction in the incidence of future major cardiovascular events.
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- 2012
26. Impact of microRNAs on regulatory networks and pathways in human colorectal carcinogenesis and development of metastasis.
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Pizzini S, Bisognin A, Mandruzzato S, Biasiolo M, Facciolli A, Perilli L, Rossi E, Esposito G, Rugge M, Pilati P, Mocellin S, Nitti D, Bortoluzzi S, and Zanovello P
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- Adenocarcinoma metabolism, Adenocarcinoma mortality, Adenocarcinoma secondary, Aged, Carcinogenesis, Colorectal Neoplasms metabolism, Colorectal Neoplasms mortality, Colorectal Neoplasms pathology, Disease Progression, Female, Gene Expression Regulation, Neoplastic, Humans, Kaplan-Meier Estimate, Liver Neoplasms metabolism, Liver Neoplasms mortality, Liver Neoplasms secondary, Male, MicroRNAs metabolism, Middle Aged, Oncogene Proteins genetics, Oncogene Proteins metabolism, Pyrophosphatases genetics, Pyrophosphatases metabolism, RNA Interference, Transcriptome, Adenocarcinoma genetics, Colorectal Neoplasms genetics, Gene Regulatory Networks, Liver Neoplasms genetics, MicroRNAs genetics
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Background: Qualitative alterations or abnormal expression of microRNAs (miRNAs) in colon cancer have mainly been demonstrated in primary tumors. Poorly overlapping sets of oncomiRs, tumor suppressor miRNAs and metastamiRs have been linked with distinct stages in the progression of colorectal cancer. To identify changes in both miRNA and gene expression levels among normal colon mucosa, primary tumor and liver metastasis samples, and to classify miRNAs into functional networks, in this work miRNA and gene expression profiles in 158 samples from 46 patients were analysed., Results: Most changes in miRNA and gene expression levels had already manifested in the primary tumors while these levels were almost stably maintained in the subsequent primary tumor-to-metastasis transition. In addition, comparing normal tissue, tumor and metastasis, we did not observe general impairment or any rise in miRNA biogenesis. While only few mRNAs were found to be differentially expressed between primary colorectal carcinoma and liver metastases, miRNA expression profiles can classify primary tumors and metastases well, including differential expression of miR-10b, miR-210 and miR-708. Of 82 miRNAs that were modulated during tumor progression, 22 were involved in EMT. qRT-PCR confirmed the down-regulation of miR-150 and miR-10b in both primary tumor and metastasis compared to normal mucosa and of miR-146a in metastases compared to primary tumor. The upregulation of miR-201 in metastasis compared both with normal and primary tumour was also confirmed. A preliminary survival analysis considering differentially expressed miRNAs suggested a possible link between miR-10b expression in metastasis and patient survival. By integrating miRNA and target gene expression data, we identified a combination of interconnected miRNAs, which are organized into sub-networks, including several regulatory relationships with differentially expressed genes. Key regulatory interactions were validated experimentally. Specific mixed circuits involving miRNAs and transcription factors were identified and deserve further investigation. The suppressor activity of miR-182 on ENTPD5 gene was identified for the first time and confirmed in an independent set of samples., Conclusions: Using a large dataset of CRC miRNA and gene expression profiles, we describe the interplay of miRNA groups in regulating gene expression, which in turn affects modulated pathways that are important for tumor development.
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- 2013
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27. Percutaneous treatment of patients with heart diseases: selection, guidance and follow-up. A review.
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Contaldi C, Losi MA, Rapacciuolo A, Prastaro M, Lombardi R, Parisi V, Parrella LS, Di Nardo C, Giamundo A, Puglia R, Esposito G, Piscione F, and Betocchi S
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- Humans, Prognosis, Treatment Outcome, Cardiovascular Diseases diagnostic imaging, Cardiovascular Diseases surgery, Echocardiography methods, Surgery, Computer-Assisted methods, Ultrasonography, Interventional methods
- Abstract
Aortic stenosis and mitral regurgitation, patent foramen ovale, interatrial septal defect, atrial fibrillation and perivalvular leak, are now amenable to percutaneous treatment. These percutaneous procedures require the use of Transthoracic (TTE), Transesophageal (TEE) and/or Intracardiac echocardiography (ICE). This paper provides an overview of the different percutaneous interventions, trying to provide a systematic and comprehensive approach for selection, guidance and follow-up of patients undergoing these procedures, illustrating the key role of 2D echocardiography.
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- 2012
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28. The G protein regulators EGL-10 and EAT-16, the Giα GOA-1 and the G(q)α EGL-30 modulate the response of the C. elegans ASH polymodal nociceptive sensory neurons to repellents.
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Esposito G, Amoroso MR, Bergamasco C, Di Schiavi E, and Bazzicalupo P
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- Animals, Animals, Genetically Modified, Caenorhabditis elegans physiology, Calcium metabolism, Copper, DNA Primers genetics, Models, Biological, Nociceptors metabolism, Quinine, Synaptic Transmission physiology, Caenorhabditis elegans metabolism, Caenorhabditis elegans Proteins metabolism, GTP-Binding Protein Regulators metabolism, GTP-Binding Protein alpha Subunits, Gi-Go metabolism, GTP-Binding Protein alpha Subunits, Gq-G11 metabolism, Nociceptors drug effects, RGS Proteins metabolism, Signal Transduction physiology
- Abstract
Background: Polymodal, nociceptive sensory neurons are key cellular elements of the way animals sense aversive and painful stimuli. In Caenorhabditis elegans, the polymodal nociceptive ASH sensory neurons detect aversive stimuli and release glutamate to generate avoidance responses. They are thus useful models for the nociceptive neurons of mammals. While several molecules affecting signal generation and transduction in ASH have been identified, less is known about transmission of the signal from ASH to downstream neurons and about the molecules involved in its modulation., Results: We discovered that the regulator of G protein signalling (RGS) protein, EGL-10, is required for appropriate avoidance responses to noxious stimuli sensed by ASH. As it does for other behaviours in which it is also involved, egl-10 interacts genetically with the G(o)/(i)α protein GOA-1, the G(q)α protein EGL-30 and the RGS EAT-16. Genetic, behavioural and Ca²(+) imaging analyses of ASH neurons in live animals demonstrate that, within ASH, EGL-10 and GOA-1 act downstream of stimulus-evoked signal transduction and of the main transduction channel OSM-9. EGL-30 instead appears to act upstream by regulating Ca²(+) transients in response to aversive stimuli. Analysis of the delay in the avoidance response, of the frequency of spontaneous inversions and of the genetic interaction with the diacylglycerol kinase gene, dgk-1, indicate that EGL-10 and GOA-1 do not affect signal transduction and neuronal depolarization in response to aversive stimuli but act in ASH to modulate downstream transmission of the signal., Conclusions: The ASH polymodal nociceptive sensory neurons can be modulated not only in their capacity to detect stimuli but also in the efficiency with which they respond to them. The Gα and RGS molecules studied in this work are conserved in evolution and, for each of them, mammalian orthologs can be identified. The discovery of their role in the modulation of signal transduction and signal transmission of nociceptors may help us to understand how pain is generated and how its control can go astray (such as chronic pain) and may suggest new pain control therapies.
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- 2010
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