16 results on '"Bin, Feng"'
Search Results
2. Wave-shaped microfluidic chip assisted point-of-care testing for accurate and rapid diagnosis of infections
- Author
-
Yin, Bin-Feng, Wan, Xin-Hua, Yang, Ming-Zhu, Qian, Chang-Cheng, and Sohan, A. S. M. Muhtasim Fuad
- Published
- 2022
- Full Text
- View/download PDF
3. Effect of different cardioprotective methods on extracorporeal circulation in fetal sheep: a randomized controlled trial
- Author
-
Yan, Yi-bo, Shi, Shuo, Wu, Qian-biao, Cai, Jin-sheng, and Lei, Bin-feng
- Published
- 2021
- Full Text
- View/download PDF
4. Comparison of ceramic-on-ceramic bearing vs ceramic-on-highly cross-linked polyethylene-bearing surfaces in total hip arthroplasty for avascular necrosis of femoral head: a prospective cohort study with a mid-term follow-up
- Author
-
Wenwei Qian, Shiliang Cao, Jin Jin, Yi Ren, Bin Feng, Xisheng Weng, and Jin Lin
- Subjects
Adult ,Male ,medicine.medical_specialty ,lcsh:Diseases of the musculoskeletal system ,Osteolysis ,Radiography ,Health-related quality of life ,Arthroplasty, Replacement, Hip ,Avascular necrosis ,Cohort Studies ,03 medical and health sciences ,Femoral head ,Wear rate ,0302 clinical medicine ,lcsh:Orthopedic surgery ,Femur Head Necrosis ,Bearing surfaces ,Bearing surface ,medicine ,Humans ,Orthopedics and Sports Medicine ,030212 general & internal medicine ,Prospective cohort study ,Aged ,030222 orthopedics ,business.industry ,Middle Aged ,medicine.disease ,Surgery ,lcsh:RD701-811 ,medicine.anatomical_structure ,Harris Hip Score ,Orthopedic surgery ,Total hip arthroplasty ,Female ,Hip Prosthesis ,lcsh:RC925-935 ,business ,Research Article - Abstract
Background The ideal bearing surface for patients of avascular necrosis (AVN) undergoing total hip arthroplasty (THA) remains controversial. The purpose of this study is to evaluate the clinical outcomes, health-related quality of life (HRQL), and wear of the bearing surface between ceramic-on-ceramic (CoC) and ceramic-on-highly cross-linked polyethylene (CoXPE) THA for patients of AVN after midterm follow-up. Methods We performed a retrospective case-control analysis of 93 CoC and 77 CoXPE consecutive THAs for patients of AVN. The cases were followed at a minimum 5 years follow-up (average 7 years). Harris hip score (HHS) score and bearing-related complications were assessed. The health-related quality of life (HRQL) was assessed with the Short Form 36 (SF-36). Plain radiographs and computed tomography (CT) were used for radiographic evaluation. Results Both the CoC group and CoXPE group showed statistically significant improvements in HHS scores with no difference between the two bearing surfaces. There was no significant difference as for SF-36 at the latest follow-up between two groups, except for significant higher scores in the dimensions of general health in the CoC group (75.7 vs 64.7, P = 0.032). No radiographic evidence of osteolysis and loosening was present at the final follow-up. The mean wear rate of the CoC was 0.0096 mm/year and the CoXPE was 0.047 mm/year after evaluation with reconstructed CT. Conclusions CoC THAs acts as well as CoXPE THAs for patients with femoral head avascular necrosis after midterm follow-up. CoC bearing can significantly decrease the wearing rate than CoXPE bearing.
- Published
- 2019
5. Clinical outcomes of ceramic femoral prosthesis in total knee arthroplasty: a systematic review
- Author
-
Bin Feng, Xisheng Weng, Shuai Xiang, Zeng Li, and Yan Zhao
- Subjects
musculoskeletal diseases ,medicine.medical_specialty ,Ceramics ,lcsh:Diseases of the musculoskeletal system ,Total knee arthroplasty ,Aseptic loosening ,Dentistry ,Outcomes ,Prosthesis Design ,03 medical and health sciences ,0302 clinical medicine ,lcsh:Orthopedic surgery ,Medicine ,Humans ,Orthopedics and Sports Medicine ,Revision rate ,Ceramic ,Femur ,Arthroplasty, Replacement, Knee ,Survival rate ,030203 arthritis & rheumatology ,030222 orthopedics ,Clinical Trials as Topic ,business.industry ,Prosthesis Failure ,Femoral prosthesis ,lcsh:RD701-811 ,Treatment Outcome ,visual_art ,Orthopedic surgery ,visual_art.visual_art_medium ,Surgery ,Implant ,Systematic Review ,lcsh:RC925-935 ,business - Abstract
Purpose Ceramic bearings have been widely used in total hip arthroplasty (THA), which resulted in satisfactory clinical outcomes due to the excellent tribological characteristics of the implants. However, ceramic components are not commonly used in total knee arthroplasty (TKA) because of brittleness. This study aimed to gather and analyze information regarding the clinical outcomes (including survival without revision, causes of revision, functional outcome, and incidence of loosening) and reach a definitive conclusion about the use of ceramic femoral components in total knee arthroplasty. Methods MEDLINE, EMBASE, Cochrane, and ClinicalTrials.gov databases were searched for studies that reported the clinical and/or radiological outcomes with or without survival data of ceramic TKA implants and that included more than 10 patients with a minimum of 1 year follow-up. Results From an initial sample of 147, there were 14 studies that met the inclusion criteria. Overall, there was a notable enhancement of joint function after the procedure, with a satisfactory mid- and long-term survival of the ceramic components, which is comparable to that of the conventional alloy components reported previously. In addition, the revision rate was reported to be between 0% and 14.37% according to the included studies. However, revision due to aseptic loosening, wear, and component fracture appeared to be rare, demonstrating the safety of in vivo use of ceramic bearings in the TKA procedure. Conclusions Ceramic TKA implants show similar post-operative clinical results and survival rate compared to their conventional metallic counterparts. Our results confirmed the safety of in vivo use of ceramic bearings in TKA, with rare implant breakage and aseptic loosening. Considering the excellent characteristics of the tribology of ceramics, the clinical use of ceramic prostheses in TKA could be promising.
- Published
- 2019
6. Mammary cell proliferation and catabolism of adipose tissues in nutrition-restricted lactating sows were associated with extracellular high glutamate levels
- Author
-
Yan Lin, Xiaoling Zhang, Jian Li, Heju Zhong, Peng Wang, De Wu, Qiaofeng Wu, Zhengfeng Fang, Yumo Song, Bin Feng, Shengyu Xu, and Lianqiang Che
- Subjects
0301 basic medicine ,Microdialysis ,medicine.medical_specialty ,Anabolism ,Lipolysis ,Adipose tissue ,Biology ,Biochemistry ,03 medical and health sciences ,Internal medicine ,Lactation ,medicine ,Extracellular ,Mammary cell proliferation ,lcsh:SF1-1100 ,lcsh:Veterinary medicine ,Catabolism ,Research ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Insulin sensitivity ,040201 dairy & animal science ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Postprandial ,lcsh:SF600-1100 ,Animal Science and Zoology ,lcsh:Animal culture ,Glutamate ,Food Science ,Biotechnology - Abstract
Background Persistent lactation, as the result of mammary cellular anabolism and secreting function, is dependent on substantial mobilization or catabolism of body reserves under nutritional deficiency. However, little is known about the biochemical mechanisms for nutrition-restricted lactating animals to simultaneously maintain the anabolism of mammary cells while catabolism of body reserves. In present study, lactating sows with restricted feed allowance (RFA) (n = 6), 24% feed restriction compared with the control (CON) group (n = 6), were used as the nutrition-restricted model. Microdialysis and mammary venous cannulas methods were used to monitor postprandial dynamic changes of metabolites in adipose and mammary tissues. Results At lactation d 28, the RFA group showed higher (P 0.05) between groups. Accordingly, piglet performance and milk yield did not differ (P > 0.05) between groups. It would appear that activation of Notch signaling and adequate supply of glutamate might assist mammogenesis. Conclusions Mammary cell proliferation and catabolism of adipose tissues in nutrition-restricted lactating sows were associated with extracellular high glutamate levels. Electronic supplementary material The online version of this article (10.1186/s40104-018-0293-6) contains supplementary material, which is available to authorized users.
- Published
- 2018
7. Risk assessment and management of preoperative venous thromboembolism following femoral neck fracture
- Author
-
Zenan Xia, Baozhong Zhang, Jin Lin, Jin Jin, Ke Xiao, Wei Zhu, Bin Feng, Xisheng Weng, Na Gao, Wenwei Qian, and Guixing Qiu
- Subjects
Male ,medicine.medical_specialty ,lcsh:Diseases of the musculoskeletal system ,Deep vein ,030204 cardiovascular system & hematology ,Femoral Neck Fractures ,Risk Assessment ,Fibrin Fibrinogen Degradation Products ,03 medical and health sciences ,0302 clinical medicine ,lcsh:Orthopedic surgery ,Preoperative Care ,Medicine ,Humans ,Orthopedics and Sports Medicine ,cardiovascular diseases ,Femoral neck ,Aged ,Retrospective Studies ,Aged, 80 and over ,030222 orthopedics ,business.industry ,Disease Management ,Fibrinogen ,Perioperative ,Odds ratio ,Venous Thromboembolism ,medicine.disease ,Thrombosis ,Surgery ,Pulmonary embolism ,lcsh:RD701-811 ,Venous thrombosis ,medicine.anatomical_structure ,Case-Control Studies ,Female ,lcsh:RC925-935 ,business ,Research Article - Abstract
Background Limited studies are available to investigate the prevalence of preoperative venous thromboembolism (VTE) in elderly patients with femoral neck fractures. Our primary aim was to determine the incidences of VTE and its risk or protective factors in such patient population. The secondary objective was to evaluate the need of therapeutic anticoagulation for isolated calf muscular venous thrombosis (ICMVT) prior to femoral neck fracture surgery. Methods This is a retrospective case-control study, including 301 femoral neck fracture patients who were admitted to our institution between January 2014 and March 2017. Bilateral Doppler ultrasonography was performed in each of the patients as a preoperative VTE screening. The event rate of VTE was calculated, and significant risk or protective factors were determined by using a multivariate logistic regression model. Patients with ICMVT were divided into anticoagulation and no anticoagulation groups to assess the efficacy and safety of preoperative therapeutic anticoagulation. Intraoperative blood loss, drainage volume, blood transfusion, perioperative hemoglobin change, and rate of thrombosis extension were compared between the two groups. Results The overall preoperative incidence of VTE in patients with femoral neck fracture was 18.9% (57/301), in which deep vein thrombosis (DVT) was 18.9% and pulmonary embolism (PE) was 1%. Among the DVT cases, 77.2% (44/57) were ICMVTs. Multiple fractures (odds ratio [OR] = 9.418; 95% confidence interval [CI] = 2.537 to 34.96), coexisting movement disorder (OR = 3.862; 95% CI = 1.658 to 8.993), bed rest for more than 7 days (OR = 2.082; 95% CI = 1.011 to 4.284) as well as elevated levels of D-dimer (OR = 1.019; 95% CI = 1.002 to 1.037) and fibrinogen (OR = 1.345; 95% CI = 1.008 to 1.796) led to an increase in the risk of VTE, while the recent use of antiplatelet drug (OR = 0.424; 95% CI = 0.181 to 0.995) and prophylactic anticoagulation (OR = 0.503; 95% CI = 0.263 to 0.959) decreased the risk of VTE. For the 39 patients with ICMVT undergoing femoral neck fracture surgery, there were no significant differences in the rate of thrombosis extension between anticoagulation and no anticoagulation groups, but significantly decreased postoperative hemoglobin was observed in the anticoagulation group. Conclusion Our findings showed a high prevalence of preoperative VTE in elderly patients with femoral neck fracture, with risk factors identified. We found that the most detected VTE were ICMVTs. Our study suggested that a direct surgery without preoperative use of therapeutic anticoagulation for ICMVT would not reduce the risk of thrombus extension, and the therapeutic use of anticoagulation may worsen postoperative anemia.
- Published
- 2018
8. Maternal nutrition modulates fetal development by inducing placental efficiency changes in gilts.
- Author
-
Long Che, ZhenGuo Yang, MengMeng Xu, ShengYu Xu, LianQiang Che, Yan Lin, ZhengFeng Fang, Bin Feng, Jian Li, DaiWen Chen, and De Wu
- Subjects
FETAL growth retardation ,ENERGY metabolism ,FETAL development ,MATERNAL nutrition ,PROTEIN expression ,DIAGNOSIS - Abstract
Background: Intra-uterine growth restriction (IUGR) and fetal overgrowth increase risks to postnatal health. Maternal nutrition is the major intrauterine environmental factor that alters fetal weight. However, the mechanisms underlying the effects of maternal nutrition on fetal development are not entirely clear. We developed a pig model, and using isobaric tags for relative and absolute quantification (iTRAQ), we investigated alterations in the placental proteome of gilts on a normal-energy-intake (Con) and high-energy-intake (HE) diet. Results: In the Con group, heavy and light fetuses were found at the tubal and cervical ends of the uterus respectively at 90 d of gestation. Moreover, the heavy fetuses had a higher glucose concentration than the light fetuses. However, a higher uniformity was noted in the HE group. Placental promoters between these two positions indicated that 78 and 50 differentially expressed proteins were detected in the Con and HE groups respectively. In the Con group, these proteins were involved in lipid metabolism (HADHA, AACS, CAD), nutrient transport (GLUT, SLC27A1), and energy metabolism (NDUFV1, NDUFV2, ATP5C1). However, in the HE group they mainly participated in transcriptional and translational regulation, and intracellular vesicular transport. Conclusions: Our findings revealed that maternal nutrition may alter birth weight mainly through the modulation of placental lipid and energy metabolism, which also provides a possible mechanism to explain the higher uniformity of fetal weight in gilts fed a HE diet. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
9. Transfer of β-hydroxy-β-methylbutyrate from sows to their offspring and its impact on muscle fiber type transformation and performance in pigs.
- Author
-
Haifeng Wan, Jiatao Zhu, Caimei Wu, Pan Zhou, Yong Shen, Yan Lin, Shengyu Xu, Lianqiang Che, Bin Feng, Jian Li, Zhengfeng Fang, and De Wu
- Subjects
BUTYRATES ,ANIMAL weaning ,SWINE growth ,BODY weight ,SLAUGHTERING - Abstract
Background: Previous studies suggested that supplementation of lactating sows with ß-hydroxy-ß-methylbutyrate (HMB) could improve the performance of weaning pigs, but there were little information in the muscle fiber type transformation of the offspring and the subsequent performance in pigs from weaning through finishing in response to maternal HMB consumption. The purpose of this study was to determine the effect of supplementing lactating sows with HMB on skeletal muscle fiber type transformation and growth of the offspring during d 28 and 180 after birth. A total of 20 sows according to their body weight were divided into the control (CON, n = 10) or HMB groups (HMB, n = 10). Sows in the HMB group were supplemented with ß-hydroxy-ß-methylbutyrate calcium (HMB-Ca) 2 g/kg feed during d 1 to 27 of lactation. After weaning, 48 mixed sex piglets were blocked by sow treatment and fed standard diets for post-weaning, growing, finishing periods. Growth performance was recorded during d 28 to 180 after birth. Pigs were slaughtered on d 28 (n = 6/treatment) and 180 (n = 6/treatment) postnatal, and the longissimus dorsi (LD) was collected, respectively. Results: The HMB-fed sows during lactation showed increased HMB concentration (P < 0.05) in milk and LD of weaning piglets (P < 0.05). In addition, offsprings in HMB group had a higher finishing BW and lean percentage than did pigs in CON group (P < 0.05), meanwhile, compared with pigs from sows fed the CON diet, pigs from sows fed HMB diet showed higher type II muscle fiber cross-sectional area (CSA), elevated myosin heavy chain (MyHC) IIb and Sox6 mRNA, and fast-MyHC protein levels in LD (P < 0.05). Conclusions: HMB supplemented to sow diets throughout lactation increases the levels of HMB in maternal milk and skeletal muscle of pigs during d 28 after birth and promotes subsequent performance of pigs between d 28 and 180 of age by enhancing glycolytic muscle fiber transformation. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
10. Mammary inflammatory gene expression was associated with reproductive stage and regulated by docosahexenoic acid: in vitro and in vivo studies.
- Author
-
Sen Lin, Yalin Zhang, Yanrong Long, Haifeng Wan, Lianqiang Che, Yan Lin, Shengyu Xu, Bin Feng, Jian Li, De Wu, and Zhengfeng Fang
- Subjects
GENE expression ,MASTITIS ,EPITHELIAL cells ,DOCOSAHEXAENOIC acid ,LIPOPOLYSACCHARIDES - Abstract
Background: Periparturient mastitis is the most prevalent disease affecting lactating animals. However, it has long been relied on antibiotics to deal with mastitis, leading to a potential threat to food safety. This study was aimed to investigate the expression of pro-inflammatory cytokines in mammary glands of sows around parturition when mastitis and oxidative stress usually occur, and evaluate the anti-inflammatory effect of docosahexenoic acid (DHA) in porcine mammary epithelial cells (PMEC) challenged by lipopolysaccharide (LPS). Methods: Mammary tissues and blood samples were collected from seven pregnant sows at different reproductive stages. Primarily cultured PMEC at passage 4 were assigned to four treatments: basal medium (control), basal medium with LPS (10 μg/mL) (LPS treatment), basal medium with LPS (10 μg/mL) and DHA (100 or 200 μM) (LPS + DHA treatments), and cell samples were harvested after 24 h incubation. The measurements included oxidative stress markers in blood samples and gene expression in mammary tissues and PMEC samples. Results: Serum α-tocopherol concentration was lower at parturition than at day 90 of gestation and day 28 post parturition, while serum malondialdehyde concentration was higher at day 28 post parturition than at day 90 of gestation. Higher interleukin (IL)-1β mRNA abundance while lower LPS binding protein mRNA abundance in mammary tissues were observed at day 90 of gestation compared with that at parturition and at day 28 and 35 post parturition. Mammary tumor necrosis factor (TNF)-α mRNA abundance were lower at parturition than at day 90 of gestation and day 28 and 35 post parturition, whereas mammary IL-8 mRNA abundance were lower at parturition than at day 35 post parturition. In the PMEC experiment, compared with the control, increased mRNA abundances of Toll-like receptor (TLR)-4 downstream target, myeloid differentiation factor 88 (MyD88), IL-6 and IL-8 were observed in LPS treatment, whereas DHA appeared to decrease mRNA abundances of MyD88, IL-6 and IL-8 induced by LPS. Conclusions: The down-regulated expression of pro-inflammatory cytokines in mammary tissues and aggravated systemic oxidative stress at parturition suggest that sows are in a vulnerable status during periparturient period. DHA appears to attenuate inflammatory responses in LPS-challenged PMEC through modulation of TLR4 signalling pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
11. Maternal high fat intake affects the development and transcriptional profile of fetal intestine in late gestation using pig model.
- Author
-
Lianqiang Che, Peilin Liu, Zhengguo Yang, Long Che, Liang Hu, Linlin Qin, Ru Wang, Zhengfeng Fang, Yan Lin, Shengyu Xu, Bin Feng, Jian Li, and De Wu
- Subjects
HIGH-fat diet ,INTESTINAL development ,MATERNAL nutrition ,FAT ,LACTASE ,IMMUNE response ,COLON cancer - Abstract
Background: The objective of this study was to investigate the effects of maternal high fat intake on intestinal development and transcriptional profile. Methods: Eight gilts with similar age and body weight were randomly allocated into 2 groups receiving the control and high fat diets (HF diet) from d 30 to 90 of gestation, with 4 gilts each group and one gilt each pen. At d 90 of gestation, two fetuses each gilt were removed by cesarean section. Intestinal samples were collected for analysis of morphology, enzyme activities and transcriptional profile. Results: The results showed that feeding HF diet markedly increased the fetal weight and lactase activity, also tended to increase intestinal morphology. Porcine Oligo Microarray analysis indicated that feeding HF diet inhibited 64 % of genes (39 genes down-regulated while 22 genes up-regulated),which were related to immune response, cancer and metabolism, also markedly modified 33 signal pathways such as antigen processing and presentation, intestinal immune network for IgA production, Jak-STAT and TGF-ß signaling transductions, pathways in colorectal cancer and glycerolipid metabolism. Conclusion: Collectively, it could be concluded that maternal high fat intake was able to increase fetal weight and lactase activity, however, it altered the intestinal immune response, signal transduction and metabolism. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
12. Cross-linked versus conventional polyethylene for total knee arthroplasty: a meta-analysis.
- Author
-
Bin-feng Yu, Guo-jing Yang, Wei-liang Wang, Lei Zhang, and Xi-peng Lin
- Subjects
- *
INFORMATION storage & retrieval systems , *MEDICAL databases , *MEDICAL information storage & retrieval systems , *MEDLINE , *META-analysis , *ONLINE information services , *POLYETHYLENE , *QUALITY assurance , *RESEARCH funding , *TOTAL knee replacement - Abstract
Background: Highly cross-linked polyethylene (HXLPE) has been reported as an effective material for decreasing polyethylene wear and osteolysis in total knee arthroplasty (TKA). Because no single study to date has been large enough to definitively determine the benefit of HXLPE in TKA, we conducted a meta-analysis to pool the results from randomized controlled trials (RCTs) and non-RCTs to make such a determination. Methods: Potential candidate articles were identified by searching the Cochrane Library, Medline (1966-2015.10), PubMed (1966-2015.10), Embase (1980-2015.10), ScienceDirect (1985-2015.10), and other databases. "Gray studies" were identified from the included articles' reference lists. Pooled data were analyzed using RevMan 5.1. Results: Three RCTs and three non-RCTs were included in the meta-analysis. There were no significant differences between the groups in the total number of reoperations (P = 0.11), reoperations for prosthesis loosening (P = 0.08), radiolucent line (P = 0.20), osteolysis (P = 0.38), prosthesis loosening (P=0.10), and mechanical failures related to the tibial polyethylene (P= 1.00). Similarly, no significant differences between the two groups were found in postoperative total knee score (P = 0.18) or functional score (P = 0.23). Conclusions: The meta-analysis showed that compared with conventional polyethylene, HXLPE did not improve the clinical and radiographic outcomes in mid-term follow-up after TKA. Additional high-quality multicenter prospective RCTs with good design, large study populations and long-term follow-up will be necessary to further clarify the effect of HXLPE in TKA. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
13. Chinese medicines in the treatment of experimental diabetic nephropathy.
- Author
-
Jing Yi Liu, Xiao Xin Chen, Chi Wai Tang, Sydney, Cho Wing Sze, Stephen, Yi Bin Feng, Kai Fai Lee, and Yan Bo Zhang, Kalin
- Subjects
DIABETES complications ,PREVENTION of disease progression ,DIABETIC nephropathies ,HERBAL medicine ,MEDICAL care costs ,CHINESE medicine ,SYSTEMATIC reviews - Abstract
Diabetic nephropathy (DN) is a severe micro vascular complication accompanying diabetes mellitus that affects millions of people worldwide. End stage renal disease occurs in nearly half of all DN patients, resulting in large medical costs and lost productivity. The course of DN progression is complicated, and effective and safe therapeutic strategies are desired. While the complex nature of DN renders medicines with a single therapeutic target less efficacious, Chinese medicine, with its holistic view targeting the whole system of the patient, has exhibited efficacy for DN management. This review aims to describe the experimental evidence for Chinese medicines in DN management, with an emphasis on the underlying mechanisms, and to discuss the combined use of herbs and drugs in DN treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
14. Analgesic effects of NB001 on mouse models of arthralgia.
- Author
-
Zhen Tian, Dong-sheng Wang, Xin-shang Wang, Jiao Tian, Jing Han, Yan-yan Guo, Bin Feng, Nan Zhang, Ming-gao Zhao, and Shui-bing Liu
- Subjects
ANALGESICS ,LABORATORY mice ,ADENYLATE cyclase ,JOINT injuries ,ARTHRITIS diagnosis ,ANTI-inflammatory agents - Abstract
Our previous studies have demonstrated the critical roles of calcium-stimulated adenylyl cyclase 1 (AC1) in the central nervous system in chronic pain. In the present study, we examined the analgesic effects of NB001, a selective inhibitor of AC1, on animal models of ankle joint arthritis and knee joint arthritis induced by complete Freund's adjuvant injection. NB001 treatment had no effect on joint edema, stiffness, and joint destruction. Furthermore, the treatment failed to attenuate the disease progression of arthritis. However, NB001 treatment (3 mg/kg) significantly weakened joint pain-related behavior in the mouse models of ankle joint arthritis and knee joint arthritis. Results indicated that NB001 exhibited an analgesic effect on the animal models of arthritis but was not caused by anti-inflammatory activities. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
15. Cross-linked versus conventional polyethylene for total knee arthroplasty: a meta-analysis.
- Author
-
Yu BF, Yang GJ, Wang WL, Zhang L, and Lin XP
- Subjects
- Arthroplasty, Replacement, Knee adverse effects, Arthroplasty, Replacement, Knee methods, Cross-Linking Reagents, Humans, Osteolysis etiology, Prosthesis Design, Prosthesis Failure, Reoperation statistics & numerical data, Arthroplasty, Replacement, Knee instrumentation, Knee Prosthesis, Polyethylene chemistry
- Abstract
Background: Highly cross-linked polyethylene (HXLPE) has been reported as an effective material for decreasing polyethylene wear and osteolysis in total knee arthroplasty (TKA). Because no single study to date has been large enough to definitively determine the benefit of HXLPE in TKA, we conducted a meta-analysis to pool the results from randomized controlled trials (RCTs) and non-RCTs to make such a determination., Methods: Potential candidate articles were identified by searching the Cochrane Library, Medline (1966-2015.10), PubMed (1966-2015.10), Embase (1980-2015.10), ScienceDirect (1985-2015.10), and other databases. "Gray studies" were identified from the included articles' reference lists. Pooled data were analyzed using RevMan 5.1., Results: Three RCTs and three non-RCTs were included in the meta-analysis. There were no significant differences between the groups in the total number of reoperations (P = 0.11), reoperations for prosthesis loosening (P = 0.08), radiolucent line (P = 0.20), osteolysis (P = 0.38), prosthesis loosening (P = 0.10), and mechanical failures related to the tibial polyethylene (P = 1.00). Similarly, no significant differences between the two groups were found in postoperative total knee score (P = 0.18) or functional score (P = 0.23)., Conclusions: The meta-analysis showed that compared with conventional polyethylene, HXLPE did not improve the clinical and radiographic outcomes in mid-term follow-up after TKA. Additional high-quality multicenter prospective RCTs with good design, large study populations and long-term follow-up will be necessary to further clarify the effect of HXLPE in TKA.
- Published
- 2016
- Full Text
- View/download PDF
16. Cloning, chromosome mapping and expression pattern of porcine PLIN and M6PRBP1 genes.
- Author
-
Tao X, Jihong Y, Li G, Bin F, Yi Z, Xiaodong C, Peichao Z, and Yang Z
- Subjects
- Animals, Carrier Proteins, Cloning, Molecular, Cyclic AMP-Dependent Protein Kinases metabolism, DNA, Complementary genetics, DNA-Binding Proteins chemistry, Molecular Sequence Data, Perilipin-1, Phosphoproteins chemistry, Phosphorylation, Phylogeny, Protein Structure, Tertiary, RNA, Messenger genetics, RNA, Messenger metabolism, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Chromosome Mapping, DNA-Binding Proteins genetics, Gene Expression Profiling, Gene Expression Regulation, Phosphoproteins genetics, Sus scrofa genetics
- Abstract
The PAT proteins, named after the three PLIN/ADRP/TIP47 (PAT) proteins, PLIN for perilipin, ADRP for adipose differentiation-related protein and TIP47 for tail-interacting protein of 47 kDa, now officially named M6PRBP1 for mannose-6-phosphate receptor binding protein 1, is a set of intracellular lipid droplet binding proteins. They are localized in the outer membrane monolayer enveloping lipid droplets and are involved in the metabolism of intracellular lipid. This work describes the cloning and sequencing of porcine PLIN and M6PRBP1 cDNAs, the chromosome mapping of these two genes, as well as the expression pattern of porcine PAT genes. Sequence analysis shows that the porcine PLIN cDNA contains an open reading frame of 1551 bp encoding 516 amino acids and that the porcine M6PRBP1 cDNA contains a coding region of 1320 bp encoding 439 amino acids. Comparison of PLIN and M6PRBP1 amino-acid sequences among various species reveals that porcine and bovine proteins are the most conserved. Porcine PLIN and M6PRBP1 genes have been mapped to pig chromosomes 7 and 2, respectively, by radiation hybrid analysis using the IMpRH panel. Expression analyses in pig showed a high expression of PLIN mRNA in adipose tissue, M6PRBP1 mRNA in small intestine, kidney and spleen and ADRP mRNA in adipose tissue, lung and spleen.
- Published
- 2008
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.