1. Extracting multiple surfaces from 3D microscopy images in complex biological tissues with the Zellige software tool.
- Author
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Trébeau C, de Monvel JB, Altay G, Tinevez JY, and Etournay R
- Subjects
- Image Processing, Computer-Assisted methods, Imaging, Three-Dimensional methods, Software, Algorithms, Microscopy methods
- Abstract
Background: Efficient tools allowing the extraction of 2D surfaces from 3D-microscopy data are essential for studies aiming to decipher the complex cellular choreography through which epithelium morphogenesis takes place during development. Most existing methods allow for the extraction of a single and smooth manifold of sufficiently high signal intensity and contrast, and usually fail when the surface of interest has a rough topography or when its localization is hampered by other surrounding structures of higher contrast. Multiple surface segmentation entails laborious manual annotations of the various surfaces separately., Results: As automating this task is critical in studies involving tissue-tissue or tissue-matrix interaction, we developed the Zellige software, which allows the extraction of a non-prescribed number of surfaces of varying inclination, contrast, and texture from a 3D image. The tool requires the adjustment of a small set of control parameters, for which we provide an intuitive interface implemented as a Fiji plugin., Conclusions: As a proof of principle of the versatility of Zellige, we demonstrate its performance and robustness on synthetic images and on four different types of biological samples, covering a wide range of biological contexts., (© 2022. The Author(s).)
- Published
- 2022
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