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Your search keyword '"Adrian-Kalchhauser, Irene"' showing total 5 results
Start Over Author "Adrian-Kalchhauser, Irene" Publisher biomed central
5 results on '"Adrian-Kalchhauser, Irene"'

4. The mitochondrial genome sequences of the round goby and the sand goby reveal patterns of recent evolution in gobiid fish.

Author

Adrian-Kalchhauser, Irene, Svensson, Ola, Kutschera, Verena E., Rosenblad, Magnus Alm, Pippel, Martin, Winkler, Sylke, Schloissnig, Siegfried, Blomberg, Anders, and Burkhardt-Holm, Patricia

Subjects
*FISH genomes, *GOBIIDAE, *NUCLEOTIDE sequencing, *MITOCHONDRIAL DNA, *NEOGOBIUS fluviatilis, *NUCLEOTIDE sequence
Abstract

Background: Vertebrate mitochondrial genomes are optimized for fast replication and low cost of RNA expression. Accordingly, they are devoid of introns, are transcribed as polycistrons and contain very little intergenic sequences. Usually, vertebrate mitochondrial genomes measure between 16.5 and 17 kilobases (kb). Results: During genome sequencing projects for two novel vertebrate models, the invasive round goby and the sand goby, we found that the sand goby genome is exceptionally small (16.4 kb), while the mitochondrial genome of the round goby is much larger than expected for a vertebrate. It is 19 kb in size and is thus one of the largest fish and even vertebrate mitochondrial genomes known to date. The expansion is attributable to a sequence insertion downstream of the putative transcriptional start site. This insertion carries traces of repeats from the control region, but is mostly novel. To get more information about this phenomenon, we gathered all available mitochondrial genomes of Gobiidae and of nine gobioid species, performed phylogenetic analyses, analysed gene arrangements, and compared gobiid mitochondrial genome sizes, ecological information and other species characteristics with respect to the mitochondrial phylogeny. This allowed us amongst others to identify a unique arrangement of tRNAs among Ponto-Caspian gobies. Conclusions: Our results indicate that the round goby mitochondrial genome may contain novel features. Since mitochondrial genome organisation is tightly linked to energy metabolism, these features may be linked to its invasion success. Also, the unique tRNA arrangement among Ponto-Caspian gobies may be helpful in studying the evolution of this highly adaptive and invasive species group. Finally, we find that the phylogeny of gobiids can be further refined by the use of longer stretches of linked DNA sequence. [ABSTRACT FROM AUTHOR]

Published
2017
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5. The round goby genome provides insights into mechanisms that may facilitate biological invasions

Author

Adrian-Kalchhauser, Irene, Blomberg, Anders, Larsson, Tomas, Musilova, Zuzana, Peart, Claire R, Pippel, Martin, Solbakken, Monica Hongroe, Suurväli, Jaanus, Walser, Jean-Claude, Wilson, Joanna Yvonne, Alm Rosenblad, Magnus, Burguera, Demian, Gutnik, Silvia, Michiels, Nico, Töpel, Mats, Pankov, Kirill, Schloissnig, Siegfried, and Winkler, Sylke

Subjects
570 Life sciences, biology, 14. Life underwater, 500 Science
Abstract

BACKGROUND The invasive benthic round goby (Neogobius melanostomus) is the most successful temperate invasive fish and has spread in aquatic ecosystems on both sides of the Atlantic. Invasive species constitute powerful in situ experimental systems to study fast adaptation and directional selection on short ecological timescales and present promising case studies to understand factors involved the impressive ability of some species to colonize novel environments. We seize the unique opportunity presented by the round goby invasion to study genomic substrates potentially involved in colonization success. RESULTS We report a highly contiguous long-read-based genome and analyze gene families that we hypothesize to relate to the ability of these fish to deal with novel environments. The analyses provide novel insights from the large evolutionary scale to the small species-specific scale. We describe expansions in specific cytochrome P450 enzymes, a remarkably diverse innate immune system, an ancient duplication in red light vision accompanied by red skin fluorescence, evolutionary patterns of epigenetic regulators, and the presence of osmoregulatory genes that may have contributed to the round goby's capacity to invade cold and salty waters. A recurring theme across all analyzed gene families is gene expansions. CONCLUSIONS The expanded innate immune system of round goby may potentially contribute to its ability to colonize novel areas. Since other gene families also feature copy number expansions in the round goby, and since other Gobiidae also feature fascinating environmental adaptations and are excellent colonizers, further long-read genome approaches across the goby family may reveal whether gene copy number expansions are more generally related to the ability to conquer new habitats in Gobiidae or in fish.

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