5 results on '"Scoles, Glen A."'
Search Results
2. Cattle tick vaccine researchers join forces in CATVAC.
- Author
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Schetters, Theo, Bishop, Richard, Crampton, Michael, Kopáček, Petr, Lew-Tabor, Alicja, Maritz-Olivier, Christine, Miller, Robert, Mosqueda, Juan, Patarroyo, Joaquín, Rodriguez-Valle, Manuel, Scoles, Glen A., and de la Fuente, José
- Subjects
MEDICAL scientists ,CATTLE tick ,BIOLOGICAL control of cattle parasites ,RHIPICEPHALUS ,PREVENTIVE medicine ,CONFERENCES & conventions - Abstract
A meeting sponsored by the Bill & Melinda Gates Foundation was held at the Avanti Hotel, Mohammedia, Morocco, July 14-15, 2015. The meeting resulted in the formation of the Cattle Tick Vaccine Consortium (CATVAC). [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
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3. Multiple mutations in the para-sodium channel gene are associated with pyrethroid resistance in Rhipicephalus microplus from the United States and Mexico.
- Author
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Stone, Nathan E., Olafson, Pia U., Davey, Ronald B., Buckmeier, Greta, Bodine, Deanna, Sidak-Loftis, Lindsay C., Giles, John R., Duhaime, Roberta, Miller, Robert J., Mosqueda, Juan, Scoles, Glen A., Wagner, David M., and Busch, Joseph D.
- Subjects
ACARICIDE resistance ,CATTLE diseases epidemiology ,CATTLE infections ,PYRETHROIDS & the environment ,SODIUM channels - Abstract
Background Acaricide resistant Rhipicephalus microplus populations have become a major problem for many cattle producing areas of the world. Pyrethroid resistance in arthropods is typically associated with mutations in domains I, II, III, and IV of voltage-gated sodium channel genes. In R. microplus, known resistance mutations include a domain II change (C190A) in populations from Australia, Africa, and South America and a domain III mutation (T2134A) that only occurs in Mexico and the U.S. Methods We investigated pyrethroid resistance in cattle fever ticks from Texas and Mexico by estimating resistance levels in field-collected ticks using larval packet discriminating dose (DD) assays and identifying single nucleotide polymorphisms (SNPs) in the para-sodium channel gene that associated with resistance. We then developed qPCR assays for three SNPs and screened a larger set of 1,488 R. microplus ticks, representing 77 field collections and four laboratory strains, for SNP frequency. Results We detected resistance SNPs in 21 of 68 U.S. field collections and six of nine Mexico field collections. We expected to identify the domain III SNP (T2134A) at a high frequency; however, we only found it in three U.S. collections. A much more common SNP in the U.S. (detected in 19 of 21 field collections) was the C190A domain II mutation, which has never before been reported from North America. We also discovered a novel domain II SNP (T170C) in ten U.S. and two Mexico field collections. The T170C transition mutation has previously been associated with extreme levels of resistance (super-knockdown resistance) in insects. We found a significant correlation (r = 0.81) between the proportion of individuals in field collections that carried any two resistance SNPs and the percent survivorship of F1 larvae from these collections in DD assays. This relationship is accurately predicted by a simple linear regression model (R2 = 0.6635). Conclusions These findings demonstrate that multiple mutations in the para-sodium channel gene independently associate with pyrethroid resistance in R. microplus ticks, which is likely a consequence of human-induced selection. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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4. The ovarian transcriptome of the cattle tick, Rhipicephalus (Boophilus) microplus, feeding upon a bovine host infected with Babesia bovis.
- Author
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Heekin, Andrew M., Guerrero, Felix D., Bendele, Kylie G., Saldivar, Leo, Scoles, Glen A., Dowd, Scot E., Gondro, Cedric, Nene, Vishvanath, Djikeng, Appolinaire, and Brayton, Kelly A.
- Subjects
CATTLE tick ,RHIPICEPHALUS ,BABESIOSIS ,APICOMPLEXA ,ANTISENSE DNA ,CATTLE - Abstract
Background: Cattle babesiosis is a tick-borne disease of cattle with the most severe form of the disease caused by the apicomplexan, Babesia bovis. Babesiosis is transmitted to cattle through the bite of infected cattle ticks of the genus Rhipicephalus. The most prevalent species is Rhipicephalus (Boophilus) microplus, which is distributed throughout the tropical and subtropical countries of the world. The transmission of B. bovis is transovarian and a previous study of the R. microplus ovarian proteome identified several R. microplus proteins that were differentially expressed in response to infection. Through various approaches, we studied the reaction of the R. microplus ovarian transcriptome in response to infection by B. bovis. Methods: A group of ticks were allowed to feed on a B. bovis-infected splenectomized calf while a second group fed on an uninfected splenectomized control calf. RNA was purified from dissected adult female ovaries of both infected and uninfected ticks and a subtracted B. bovis-infected cDNA library was synthesized, subtracting with the uninfected ovarian RNA. Four thousand ESTs were sequenced from the ovary subtracted library and annotated. Results: The subtracted library dataset assembled into 727 unique contigs and 2,161 singletons for a total of 2,888 unigenes, Microarray experiments designed to detect B. bovis-induced gene expression changes indicated at least 15 transcripts were expressed at a higher level in ovaries from ticks feeding upon the B. bovis-infected calf as compared with ovaries from ticks feeding on an uninfected calf. We did not detect any transcripts from these microarray experiments that were expressed at a lower level in the infected ovaries compared with the uninfected ovaries. Using the technique called serial analysis of gene expression, 41 ovarian transcripts from infected ticks were differentially expressed when compared with transcripts of controls. Conclusion: Collectively, our experimental approaches provide the first comprehensive profile of the R. microplus ovarian transcriptome responding to infection by B. bovis. This dataset should prove useful in molecular studies of host-pathogen interactions between this tick and its apicomplexan parasite [ABSTRACT FROM AUTHOR]
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- 2013
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5. Silencing expression of the Rhipicephalus microplus vitellogenin receptor gene blocks Babesia bovis transmission and interferes with oocyte maturation.
- Author
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Hussein, Hala E., Taus, Naomi S., Suarez, Carlos E., Scoles, Glen A., Ueti, Massaro W., and Johnson, Wendell C.
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VITELLOGENINS ,RHIPICEPHALUS ,GENE silencing ,GENE expression ,BABESIA ,OVARIES ,DEVELOPMENTAL biology ,RNA interference - Abstract
Background: Rhipicephalus microplus is an efficient biological vector of Babesia bovis, a causative agent of bovine babesiosis. Babesia bovis is passed transovarially to the next generation of ticks, which then transmit the parasite to naïve animals. Due to the importance of the R. microplus ovary for tick reproduction and transmission of B. bovis, we investigated the hypothesis that silencing vitellogenin receptor gene expression in the ovary during tick feeding on B. bovis-infected cattle would affect parasite transmission to the next generation of ticks. Results: Silencing expression of the vitellogenin receptor in the ovary by RNA interference, resulted in reduced tick fertility. We observed reduced egg production (i.e. reduced weight of eggs), a lower rate of embryonic development, and a reduction in hatching. Analysis of individual larvae by PCR confirmed that RNAi mediated downregulation of the R. microplus vitellogenin receptor and also interfered with transovarial transmission of B. bovis. None of the larvae (0/58) from the RmVgR dsRNA-injected group were PCR-positive, whereas 12% (7/58) and 17% (10/58) of larvae from the non-injected and buffer-injected control groups, respectively, were infected with B. bovis. Conclusions: The combined effects of reduced fecundity and reduced infection in surviving larvae resulting from silencing indicate that vitellogenin receptor is essential for tick reproduction and may play a vital role in B. bovis transmission. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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