1. Preparing Single-cell DNA Library Using Nextera for Detection of CNV.
- Author
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Xi L, Leong P, and Mihajlovic A
- Abstract
Single-cell DNA sequencing is a powerful tool to evaluate the state of heterogeneity of heterogeneous tissues like cancer in a quantitative manner that bulk sequencing can never achieve. DOP-PCR (Degenerate Oligonucleotide-Primed Polymerase Chain Reaction), MDA (Multiple Displacement Amplification), MALBAC (Multiple Annealing and Looping-Based Amplification Cycles), LIANTI (Linear Amplification via Transposon Insertion) and TnBC (Transposon Barcoded) have been the primary choices to prepare single-cell libraries. TnBC library prep method is a simple and versatile methodology, to detect copy number variations or to obtain the absolute copy numbers of genes per cell., Competing Interests: Competing interestsDigenomix is commercializing the technology., (Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.)
- Published
- 2019
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