1. Serial Immunoprecipitation of 3xFLAG/V5-tagged Yeast Proteins to Identify Specific Interactions with Chaperone Proteins
- Author
-
David Pincus and Xu Zheng
- Subjects
Lysis ,Immunoprecipitation ,Strategy and Management ,Protein complexes ,Peptide ,environment and public health ,Article ,Industrial and Manufacturing Engineering ,03 medical and health sciences ,0302 clinical medicine ,Affinity chromatography ,Antigen ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Yeast Proteins ,Chemistry ,Mechanical Engineering ,Metals and Alloys ,food and beverages ,Yeast ,FLAG tag ,Cell biology ,V5 tag ,030217 neurology & neurosurgery - Abstract
This method was generated to isolate high affinity protein complexes from yeast lysate by performing serial affinity purification of doubly tagged 3×FLAG/V5 proteins. First, the bait protein of interest is immunoprecipitated by anti-FLAG-conjugated magnetic beads and gently eluted by 3×FLAG antigen peptide. Next, the bait protein is recaptured from the first eluate by anti-V5-conjugated magnetic beads and eluted with ionic detergent. In this manner, the majority of abundant, nonspecific proteins remain either bound to the first beads or in the first eluate, allowing pure, high affinity complexes to be obtained. This approach can be used to show specific interactions with notoriously ‘sticky’ chaperone proteins.
- Published
- 2017