1. Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning
- Author
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Majse Nafisi, Cuiwei Wang, Nikolai Wulff, Hussam Hassan Nour-Eldin, Zeinu Mussa Belew, Morten Egevang Jørgensen, Sophie Konstanze Lambertz, and Deyang Xu
- Subjects
0106 biological sciences ,0301 basic medicine ,Cloning ,Strategy and Management ,Mechanical Engineering ,Metals and Alloys ,Uracil ,Computational biology ,Molecular cloning ,01 natural sciences ,Combinatorial chemistry ,Industrial and Manufacturing Engineering ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Plant science ,chemistry ,010608 biotechnology ,Methods Article ,Vector (molecular biology) ,In vitro recombination ,DNA - Abstract
This protocol describes how to order and directly assemble uracil-containing non-clonal DNAfragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal ofmaking synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol ishighly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained fromany synthesizing company. The protocol drastically reduces time and handling between receiving thesynthesized DNA fragments and transforming with vector and DNA fragment(s).
- Published
- 2017
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