1. Cancer-derived VEGF plays no role in malignant ascites formation in the mouse
- Author
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Fumihiko Kanai, Bayasi Guleng, Hideaki Ijichi, Kazunari Taira, Miki Ohta, Yasushi Fukushima, Jun Imamura, Yasuo Tanaka, Yoshinari Asaoka, Keisuke Tateishi, Takao Kawabe, Keita Morikane, Masao Omata, Amarsanaa Jazag, Makoto Miyagishi, and Tsuneo Ikenoue
- Subjects
Vascular Endothelial Growth Factor A ,Pathology ,medicine.medical_specialty ,Stromal cell ,Molecular Sequence Data ,Cell ,Biology ,Mice ,chemistry.chemical_compound ,Cell Line, Tumor ,medicine ,Animals ,RNA, Small Interfering ,Gene knockdown ,Base Sequence ,Peritoneal fluid ,Gastroenterology ,Ascites ,Cancer ,General Medicine ,medicine.disease ,Mice, Inbred C57BL ,Pancreatic Neoplasms ,Vascular endothelial growth factor ,Vascular endothelial growth factor A ,Basic Research ,medicine.anatomical_structure ,chemistry ,Tumor progression ,Cancer research ,RNA Interference ,Neoplasm Transplantation - Abstract
AIM: Vascular endothelial growth factor (VEGF) is a potent mediator of peritoneal fluid accumulation following tumor progression. This study investigated the role of VEGF secreted by cancerous cells in the formation of malignant ascites. METHODS: VEGF expression was eliminated by knockdown in the pancreas cancer cell-line PancO2 using vector-based short-hairpin type RNA interference (RNAi). Malignant ascites formation in the mouse was analyzed by intraperitoneal injection of PancO2 cells expressing VEGF or with expression knockdown. RESULTS: The VEGF knockdown PancO2 cell was successfully established. Knockdown of VEGF did not affect cancer cell proliferation in vitro or in vivo. The volume of ascites following peritoneal expansion of the tumor in VEGF knockdown cells and control cells did not differ statistically in this in vivo study. Moreover, the VEGF concentration in the ascites did not differ statistically. CONCLUSION: Malignant ascites formation might be mediated by VEGF production in noncancerous tissues, such as stromal compartments. An anti-VEGF strategy against malignant ascites could be applied to various tumors regardless of whether they secrete VEGF.
- Published
- 2005
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