1. Effects of glutamine supplementation on gut barrier, glutathione content and acute phase response in malnourished rats during inflammatory shock
- Author
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L. Belmonte, Maryvonne Daveau, Antony Leplingard, Philippe Ducrotté, Florence Le Pessot, Jean-François Lemeland, Moïse Coëffier, B. Hecketsweiler, Olga Miralles-Barrachina, Martine Hiron, and Pierre Déchelotte
- Subjects
Male ,medicine.medical_specialty ,Turpentine ,Glutamine ,Orosomucoid ,Jejunum ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Intestinal mucosa ,Internal medicine ,medicine ,Mesenteric lymph nodes ,Animals ,RNA, Messenger ,Intestinal Mucosa ,Acute-Phase Reaction ,Glycoproteins ,biology ,Malnutrition ,Gastroenterology ,Acute-phase protein ,General Medicine ,Glutathione ,Blood Proteins ,Inflammatory Bowel Diseases ,Blood proteins ,Rats ,Disease Models, Animal ,medicine.anatomical_structure ,Endocrinology ,Biochemistry ,chemistry ,Liver ,Bacterial Translocation ,Dietary Supplements ,biology.protein ,Irritants ,Rapid Communication ,Acute-Phase Proteins - Abstract
AIM: To evaluate the effect of glutamine on intestinal mucosa integrity, glutathione stores and acute phase response in protein-depleted rats during an inflammatory shock. METHODS: Plasma acute phase proteins (APP), jejunal APP mRNA levels, liver and jejunal glutathione concentrations were measured before and one, three and seven days after turpentine injection in 4 groups of control, protein-restricted, protein-restricted rats supplemented with glutamine or protein powder. Bacterial translocation in mesenteric lymph nodes and intestinal morphology were also assessed. RESULTS: Protein deprivation and turpentine injection significantly reduced jejunal villus height, and crypt depths. Mucosal glutathione concentration significantly decreased in protein-restricted rats. Before turpentine oil, glutamine supplementation restored villus heights and glutathione concentration (3.24 ± 1.05 vs 1.72 ± 0.46 μmol/g tissue, P < 0.05) in the jejunum, whereas in the liver glutathione remained low. Glutamine markedly increased jejunal α1-acid glycoprotein mRNA level after turpentine oil but did not affect its plasma concentration. Bacterial translocation in protein-restricted rats was not prevented by glutamine or protein powder supplementation. CONCLUSION: Glutamine restored gut glutathione stores and villus heights in malnourished rats but had no preventive effect on bacterial translocation in our model.
- Published
- 2007