1. Polyethylene glycol rinse solution: An effective way to prevent ischemia-reperfusion injury
- Author
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Mohamed Bejaoui, Joan Roselló-Catafau, Hassen Ben Abdennebi, Mohamed Amine Zaouali, María Calvo, Antoni Rimola, Gianfranco Pasut, Emma Folch-Puy, René Adam, and Eirini Pantazi
- Subjects
Male ,Adenosine ,Time Factors ,Mitochondria, Liver ,Adenosine monophosphate activated protein kinase ,Pharmacology ,medicine.disease_cause ,Polyethylene Glycols ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Liver Function Tests ,Insulin ,Heat shock protein 70 ,Rinse solution ,Cytoskeleton ,Liver injury ,medicine.diagnostic_test ,Heme oxygenase 1 ,Cold Ischemia ,Gastroenterology ,Organ Preservation ,General Medicine ,Glutathione ,Polyethylene glycol 35 ,Metalloproteinases ,Liver ,Biochemistry ,Reperfusion Injury ,Original Article ,Signal Transduction ,Allopurinol ,Organ Preservation Solutions ,Ischemia-reperfusion injury ,Nitric oxide ,Raffinose ,Autophagy ,medicine ,Animals ,Hepatectomy ,cardiovascular diseases ,Liver transplantation ,technology, industry, and agriculture ,AMPK ,medicine.disease ,PEG ,Heme oxygenase ,Disease Models, Animal ,Oxidative Stress ,chemistry ,Cytoprotection ,Liver washout ,Liver function ,Liver function tests ,Reperfusion injury ,Biomarkers ,Oxidative stress - Abstract
© 2014 Baishideng Publishing Group Inc. AIM: To test whether a new rinse solution containing polyethylene glycol 35 (PEG-35) could prevent ischemia-reperfusion injury (IRI) in liver grafts. METHODS: Sprague-Dawley rat livers were stored in University of Wisconsin preservation solution and then washed with different rinse solutions (Ringer's lactate solution and a new rinse solution enriched with PEG-35 at either 1 or 5 g/L) before ex vivo perfusion with Krebs-Heinseleit buffer solution. We assessed the following: liver injury (transaminase levels), mitochondrial damage (glutamate dehydrogenase activity), liver function (bile output and vascular resistance), oxidative stress (malondialdehyde), nitric oxide, liver autophagy (Beclin-1 and LCB3) and cytoskeleton integrity (filament and globular actin fraction); as well as levels of metalloproteinases (MMP2 and MMP9), adenosine monophosphate- Activated protein kinase (AMPK), heat shock protein 70 (HSP70) and heme oxygenase 1 (HO-1). RESULTS: When we used the PEG-35 rinse solution, reduced hepatic injury and improved liver function were noted after reperfusion. The PEG-35 rinse solution prevented oxidative stress, mitochondrial damage, and liver autophagy. Further, it increased the expression of cytoprotective heat shock proteins such as HO-1 and HSP70, activated AMPK, and contributed to the restoration of cytoskeleton integrity after IRI. CONCLUSION: Using the rinse solution containing PEG-35 was effective for decreasing liver graft vulnerability to IRI., Supported by The Ministerio de Sanidad y Consumo No. PIO81988 (Madrid, Spain); Eirini Pantazi wishes to thank the Agència de Gestió d’Ajuts Universitaris i de Recerca No.2012FI_B00382; Mohamed Bejaoui thanks CSIC No. I-COOP05 for their fellowships
- Published
- 2014